JP3624916B2 - Oocyst killers - Google Patents

Description

【００２６】
【発明の効果】
本発明によれば、コクシジウムオーシストに対して強い殺滅効果を発揮し、環境に対する影響が小さい、オーシスト殺滅剤が提供される。[0001]
[Industrial application fields]
The present invention relates to a coccidium oocyst killing agent parasitic on animals such as livestock, poultry and pets. In particular, it relates to a method for preventing chicken coccidiosis infection.
[0002]
[Prior art]
Among the diseases that are problematic in the field of livestock, coccidiosis is difficult to control because oocysts that are the source of infection have strong resistance to disinfectants. Coccidial protozoa are protozoa belonging to the genus Eimeria. In the field of livestock, the damage of chicken coccidiosis is significant. Coccidium infects animals in the state of oocysts, becomes parasites within the animal body, and then grows parasitic on intestinal epithelial cells.
[0003]
Thereafter, the worm body becomes oocyst and is released out of the animal body together with feces. The oocysts immediately after being released to the outside of the animal body have no infectivity because they do not form spores, but after several days under suitable conditions, they form spores and have infectivity.
[0004]
Since coccidiosis is transmitted by oocysts, the transmission of coccidiosis cannot be prevented unless oocysts are killed. As an antibacterial disinfectant effective against oocysts, ortho-agents mainly composed of o-dichlorobenzene are known. However, in the case of ortho-agents, there is a concern about adverse effects on the environment around the spraying place.
[0005]
Therefore, development of a drug having a strong killing effect against oocysts and low environmental toxicity is desired.
[0006]
[Problems to be solved by the present invention]
The object of the present invention is to provide an oocyst killing agent that has a strong killing effect on coccidiosis oocysts and has a small environmental impact.
[0007]
[Means for Solving the Problems]
The present inventors have conducted research on oocystic agents. As a result, it was found that a drug composed of hydrogen peroxide and a basic compound has an excellent killing effect against coccidial oocysts.
[0008]
In the present invention, a hydrogen peroxide aqueous solution or a compound that generates hydrogen peroxide when dissolved in water is used as the hydrogen peroxide. Among the compounds that generate hydrogen peroxide when dissolved in water, when using a composite of hydrogen peroxide and an inorganic salt that shows basicity when dissolved in water, the inorganic salt that constitutes the composite will be described later. It corresponds to a composition comprising hydrogen peroxide and a basic compound.
[0009]
Therefore, when using the said composite, it is not necessary to add another basic compound. Examples of such a composite of hydrogen peroxide and an inorganic salt include sodium carbonate hydrogen peroxide adduct and sodium perborate.
[0010]
As the basic compound used in the present invention, an inorganic substance or an organic substance can be used, and among them, an inorganic water-soluble salt is preferable. Examples of inorganic water-soluble salts include alkali metal hydroxides, silicates and carbonates, alkaline earth metal hydroxides, and ammonium ion carbonates and bicarbonates.
[0011]
Of these, sodium hydroxide, sodium metasilicate, sodium carbonate, ammonium carbonate, double salt of ammonium hydrogen carbonate and ammonium carbamate and the like are particularly preferable.
[0012]
Although the agent of the present invention can be used in various dosage forms, it is preferably used in an aqueous solution. When used in an aqueous solution, the pH is preferably 7.5 or higher, preferably 8.0 to 12.
[0013]
Specifically, hydrogen peroxide or a compound that generates hydrogen peroxide by making it into an aqueous solution has a hydrogen peroxide concentration of 0.001 to 35% (% by weight in the aqueous solution; the same shall apply hereinafter), preferably 0.01 The above pH condition is obtained by using an amount of 10% to 10% and using an amount of the basic compound in an amount of 0.001 to 50%, preferably 0.01 to 10%.
[0014]
By using a composite of hydrogen peroxide and an inorganic salt that shows basicity when made into an aqueous solution, an aqueous solution that meets the above pH conditions can also be obtained. In that case, a basic compound is not necessarily added.
[0015]
It is desirable to mix a compound that generates hydrogen peroxide with hydrogen peroxide or an aqueous solution and a basic compound immediately before use.
Hydrogen peroxide, which is the main component of the present invention, has low toxicity, and since it decomposes into oxygen and water after spraying, it has the feature that it does not remain in the environment and has little adverse effects on the environment.
[0016]
【Example】
The test of the killing effect on chicken coccidia protozoa oocysts was performed as follows.
Example 1
A non-spore-forming coccidium oocyst from Eimeria tenella Ie test strain was used. The oocysts were infected with spore-forming oocysts in chickens, and 7 days after infection, those excreted in the stool were collected by the sucrose suspension method and used in the following tests in common.
[0017]
0.5 ml of the suspended liquid in which the collected oocysts were suspended was added to 6 ml of an aqueous solution containing 4.5% by weight of hydrogen peroxide and 2.5% by weight of sodium metasilicate, and sensitized at 25 ° C. for 24 hours. After sensitization, the test solution was washed and removed by centrifugal washing with distilled water three times (2000 rpm, 5 minutes). After washing, oocysts were cultured for 7 days in a 2% aqueous potassium dichromate solution. After culturing, 1000 oocysts were microscopically examined to determine the number of spores formed.
[0018]
The spore formation rate was converted with the control spore formation rate as 100%, using a sensitized product that was similarly sensitized using water instead of the test solution.
[0019]
Example 2
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 4.5% by weight of hydrogen peroxide and 2.5% by weight of sodium carbonate was sprayed as a test solution.
[0020]
Example 3
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 4.5% by weight of hydrogen peroxide and 2.5% by weight of ammonium carbonate was sprayed as a test solution.
[0021]
Example 4
Measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 1.0% by weight of sodium carbonate-hydrogen peroxide adduct was sprayed as a test solution.
[0022]
Comparative Example 1
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 4.5% by weight of hydrogen peroxide and 1.0% by weight of acetic acid was sprayed as a test solution.
[0023]
Comparative Example 2
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 4.5% by weight of hydrogen peroxide and 1.0% by weight of sulfuric acid was sprayed as a test solution.
[0024]
The test result of the bactericidal effect with respect to the oocysts of Examples 1 to 4 and Comparative Examples 1 to 2 is shown in Table 1. In Examples 1-4, since oocyst sporulation is disabled, it is understood that the aqueous solution of the present invention exhibits a significant killing action on oocysts.
[0025]
[Table 1]

[0026]
【The invention's effect】
ADVANTAGE OF THE INVENTION According to this invention, the oocyst killing agent which exhibits the strong killing effect with respect to a coccidium oocyst and has little influence with respect to an environment is provided.

Claims (2)

An oocyst killing agent containing hydrogen peroxide and a water-soluble basic salt of alkali metal or alkaline earth metal as essential components. An oocyst killing agent comprising an aqueous solution containing hydrogen peroxide and a water-soluble basic salt of an alkali metal / alkaline earth metal, having a pH of 7.5 or more.