CN112515177A - Deer bone marrow small molecule peptide freeze-dried powder composition and preparation method thereof - Google Patents
Deer bone marrow small molecule peptide freeze-dried powder composition and preparation method thereof Download PDFInfo
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- CN112515177A CN112515177A CN202011551186.1A CN202011551186A CN112515177A CN 112515177 A CN112515177 A CN 112515177A CN 202011551186 A CN202011551186 A CN 202011551186A CN 112515177 A CN112515177 A CN 112515177A
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- 241000282994 Cervidae Species 0.000 title claims abstract description 81
- 210000001185 bone marrow Anatomy 0.000 title claims abstract description 71
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 47
- 239000000843 powder Substances 0.000 title claims abstract description 37
- 150000003384 small molecules Chemical class 0.000 title claims abstract description 29
- 239000000203 mixture Substances 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims description 10
- 102000008186 Collagen Human genes 0.000 claims abstract description 7
- 108010035532 Collagen Proteins 0.000 claims abstract description 7
- 240000001008 Dimocarpus longan Species 0.000 claims abstract description 7
- 235000000235 Euphoria longan Nutrition 0.000 claims abstract description 7
- 235000017784 Mespilus germanica Nutrition 0.000 claims abstract description 7
- 244000182216 Mimusops elengi Species 0.000 claims abstract description 7
- 235000000560 Mimusops elengi Nutrition 0.000 claims abstract description 7
- 235000007837 Vangueria infausta Nutrition 0.000 claims abstract description 7
- 239000002775 capsule Substances 0.000 claims abstract description 7
- 229920001436 collagen Polymers 0.000 claims abstract description 7
- 235000020710 ginseng extract Nutrition 0.000 claims abstract description 7
- 239000004365 Protease Substances 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 238000007710 freezing Methods 0.000 claims description 12
- 230000008014 freezing Effects 0.000 claims description 12
- 238000003860 storage Methods 0.000 claims description 12
- 108090000145 Bacillolysin Proteins 0.000 claims description 6
- 108091005658 Basic proteases Proteins 0.000 claims description 6
- 108010004032 Bromelains Proteins 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 108091005507 Neutral proteases Proteins 0.000 claims description 6
- 102000035092 Neutral proteases Human genes 0.000 claims description 6
- 108090000526 Papain Proteins 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 235000019835 bromelain Nutrition 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 230000006837 decompression Effects 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 230000000249 desinfective effect Effects 0.000 claims description 6
- 229940088598 enzyme Drugs 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 235000019834 papain Nutrition 0.000 claims description 6
- 229940055729 papain Drugs 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 241000382455 Angelica sinensis Species 0.000 claims description 5
- 235000001287 Guettarda speciosa Nutrition 0.000 abstract description 2
- 244000061520 Angelica archangelica Species 0.000 abstract 1
- 210000000988 bone and bone Anatomy 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000007812 deficiency Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000032683 aging Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 241000125175 Angelica Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008468 bone growth Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 229940088592 immunologic factor Drugs 0.000 description 1
- 239000000367 immunologic factor Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The deer bone marrow small molecule peptide freeze-dried powder composition is characterized by being combined according to parts by weight, the total weight is 100 parts, and the components are as follows: 40-50 parts of deer bone marrow small molecular peptide freeze-dried powder, 20-25 parts of deer skin collagen peptide freeze-dried powder, 5-10 parts of ginseng extract, 5-15 parts of angelica extract, 5-15 parts of longan extract and 5-15 parts of medlar extract, and the composition is prepared into capsules or tablets.
Description
Technical Field
The invention discloses a deer bone marrow small molecule peptide freeze-dried powder composition and a preparation method thereof, belonging to the field of food processing.
Background
The deer bone can be used as a medicine for the whole body, is rare medicinal material, and has the effects of tonifying deficiency, strengthening tendons and bones. Amino acids, various protein polypeptides, various mineral substances and vitamins contained in the deer bones are various nutrient substances required by human bones. The deer bone marrow contains the nutrient components of the deer bone, active hematopoietic factors and immune factors, and is beneficial to enriching blood and tonifying deficiency. In order to better exert the effects of supplementing calcium, tonifying deficiency, tonifying kidney and strengthening bones, the deer bone marrow protein is hydrolyzed into small molecular peptide, the small molecular peptide has simple structure and small molecular weight, can be quickly absorbed through small intestinal mucosa without being digested again, does not consume energy, is efficient and complete in absorption, conversion and utilization, can provide nutrient substances required by the growth and development of a human body, has special biological functions, can prevent and treat thrombus, hyperlipidemia and hypertension, delays aging, resists fatigue and improves the immunity of the organism. And the hydrolysis of deer bone marrow protein does not affect the biological activity of other small molecular substances. Most of deer bone marrow on the market is directly applied without hydrolysis treatment at present, the treatment mode is not favorable for full play and utilization of the nutritional value, and the prior art does not have a health food of deer bone marrow micromolecule peptide.
Disclosure of Invention
In order to solve the defects, the invention provides a deer bone marrow small molecule peptide freeze-dried powder composition and a preparation method thereof.
The technical scheme adopted by the invention for solving the technical problem is as follows:
the deer bone marrow small molecule peptide freeze-dried powder composition is characterized by being combined according to parts by weight, the total weight is 100 parts, and the components are as follows: 40-50 parts of deer bone marrow small molecular peptide freeze-dried powder, 20-25 parts of deer skin collagen peptide freeze-dried powder, 5-10 parts of ginseng extract, 5-15 parts of angelica sinensis extract, 5-15 parts of longan extract and 5-15 parts of medlar extract, and the composition is prepared into capsules or tablets.
A preparation method of a deer bone marrow small molecule peptide freeze-dried powder composition comprises the following steps: step one, disinfecting and cleaning fresh deer bone marrow, and then quickly freezing to-18 to-40 ℃ for storage; step two, unfreezing and weighing the frozen deer bone marrow, adding deionized water which is 4-6 times of the weight of the unfrozen deer bone marrow, and homogenizing under the high pressure of 20-40 Mpa; step three, adding at least one of neutral protease, alkaline protease, papain and bromelain into the homogenized deer bone marrow solution for ultrasonic enzymolysis, wherein the enzymolysis conditions are as follows: adding enzyme at 4000U-6000U/g based on deer bone marrow, ultrasonic power of 500W, enzymolysis temperature of 50-60 deg.C, enzymolysis time of 4-6h, enzymolysis pH of 7-8, and enzyme-inactivating treatment at 100 deg.C for 10-20min to obtain deer bone marrow small molecule peptide solution; step four, refrigerating and storing the solution obtained in the step three for 24 hours, separating and removing upper fat, and filtering the solution through PP cotton; step five, adding a proper amount of active carbon and diatomite into the solution obtained in the step four, uniformly stirring, filtering by a 0.45-micrometer filter membrane to remove impurities, degerming and decoloring to obtain filtrate; step six, concentrating the filtrate obtained in the step five under reduced pressure to 25-40% of the original volume to obtain a concentrated solution; and seventhly, pre-freezing the concentrated solution obtained in the sixth step at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of 1-10pa and-40 to-60 ℃ to obtain the deer bone marrow small molecule peptide freeze-dried powder, and carrying out low-temperature sealed storage at the temperature of-20 ℃.
The invention has the following beneficial effects: has effects in improving immunity, delaying aging of human cells, resisting oxidation, protecting cardiovascular and cerebrovascular system, resisting inflammation and ulcer, promoting bone growth, and preventing anemia, and is a high-value nutrient.
Detailed Description
The deer bone marrow small molecule peptide freeze-dried powder composition is characterized by being combined according to parts by weight, the total weight is 100 parts, and the components are as follows: 40-50 parts of deer bone marrow small molecular peptide freeze-dried powder, 20-25 parts of deer skin collagen peptide freeze-dried powder, 5-10 parts of ginseng extract, 5-15 parts of angelica sinensis extract, 5-15 parts of longan extract and 5-15 parts of medlar extract, and the composition is prepared into capsules or tablets.
A preparation method of a deer bone marrow small molecule peptide freeze-dried powder composition comprises the following steps: step one, disinfecting and cleaning fresh deer bone marrow, and then quickly freezing to-18 to-40 ℃ for storage; step two, unfreezing and weighing the frozen deer bone marrow, adding deionized water which is 4-6 times of the weight of the unfrozen deer bone marrow, and homogenizing under the high pressure of 20-40 Mpa; step three, adding at least one of neutral protease, alkaline protease, papain and bromelain into the homogenized deer bone marrow solution for ultrasonic enzymolysis, wherein the enzymolysis conditions are as follows: adding enzyme at 4000U-6000U/g based on deer bone marrow, ultrasonic power of 500W, enzymolysis temperature of 50-60 deg.C, enzymolysis time of 4-6h, enzymolysis pH of 7-8, and enzyme-inactivating treatment at 100 deg.C for 10-20min to obtain deer bone marrow small molecule peptide solution; step four, refrigerating and storing the solution obtained in the step three for 24 hours, separating and removing upper fat, and filtering the solution through PP cotton; step five, adding a proper amount of active carbon and diatomite into the solution obtained in the step four, uniformly stirring, filtering by a 0.45-micrometer filter membrane to remove impurities, degerming and decoloring to obtain filtrate; step six, concentrating the filtrate obtained in the step five under reduced pressure to 25-40% of the original volume to obtain a concentrated solution; and seventhly, pre-freezing the concentrated solution obtained in the sixth step at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of 1-10pa and-40 to-60 ℃ to obtain the deer bone marrow small molecule peptide freeze-dried powder, and carrying out low-temperature sealed storage at the temperature of-20 ℃.
Example 1
The deer bone marrow small molecule peptide freeze-dried powder composition is characterized by being combined according to parts by weight, the total weight is 100 parts, and the components are as follows: 40 parts of deer bone marrow small molecular peptide freeze-dried powder, 20 parts of deer skin collagen peptide freeze-dried powder, 5 parts of ginseng extract, 10 parts of angelica sinensis extract, 10 parts of longan extract and 15 parts of medlar extract, and the composition is prepared into capsules or tablets.
A preparation method of a deer bone marrow small molecule peptide freeze-dried powder composition comprises the following steps: step one, disinfecting and cleaning fresh deer bone marrow, and then quickly freezing to-18 to-40 ℃ for storage; step two, unfreezing and weighing the frozen deer bone marrow, adding deionized water which is 4-6 times of the weight of the unfrozen deer bone marrow, and homogenizing under the high pressure of 20-40 Mpa; step three, adding at least one of neutral protease, alkaline protease, papain and bromelain into the homogenized deer bone marrow solution for ultrasonic enzymolysis, wherein the enzymolysis conditions are as follows: adding enzyme at 4000U-6000U/g based on deer bone marrow, ultrasonic power of 500W, enzymolysis temperature of 50-60 deg.C, enzymolysis time of 4-6h, enzymolysis pH of 7-8, and enzyme-inactivating treatment at 100 deg.C for 10-20min to obtain deer bone marrow small molecule peptide solution; step four, refrigerating and storing the solution obtained in the step three for 24 hours, separating and removing upper fat, and filtering the solution through PP cotton; step five, adding a proper amount of active carbon and diatomite into the solution obtained in the step four, uniformly stirring, filtering by a 0.45-micrometer filter membrane to remove impurities, degerming and decoloring to obtain filtrate; step six, concentrating the filtrate obtained in the step five under reduced pressure to 25-40% of the original volume to obtain a concentrated solution; and seventhly, pre-freezing the concentrated solution obtained in the sixth step at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of 1-10pa and-40 to-60 ℃ to obtain the deer bone marrow small molecule peptide freeze-dried powder, and carrying out low-temperature sealed storage at the temperature of-20 ℃.
Example 2
The deer bone marrow small molecule peptide freeze-dried powder composition is characterized by being combined according to parts by weight, the total weight is 100 parts, and the components are as follows: 45 parts of deer bone marrow small molecular peptide freeze-dried powder, 25 parts of deer skin collagen peptide freeze-dried powder, 5 parts of ginseng extract, 5 parts of angelica sinensis extract, 10 parts of longan extract and 15 parts of medlar extract, and the composition is prepared into capsules or tablets.
A preparation method of a deer bone marrow small molecule peptide freeze-dried powder composition comprises the following steps: step one, disinfecting and cleaning fresh deer bone marrow, and then quickly freezing to-18 to-40 ℃ for storage; step two, unfreezing and weighing the frozen deer bone marrow, adding deionized water which is 4-6 times of the weight of the unfrozen deer bone marrow, and homogenizing under the high pressure of 20-40 Mpa; step three, adding at least one of neutral protease, alkaline protease, papain and bromelain into the homogenized deer bone marrow solution for ultrasonic enzymolysis, wherein the enzymolysis conditions are as follows: adding enzyme at 4000U-6000U/g based on deer bone marrow, ultrasonic power of 500W, enzymolysis temperature of 50-60 deg.C, enzymolysis time of 4-6h, enzymolysis pH of 7-8, and enzyme-inactivating treatment at 100 deg.C for 10-20min to obtain deer bone marrow small molecule peptide solution; step four, refrigerating and storing the solution obtained in the step three for 24 hours, separating and removing upper fat, and filtering the solution through PP cotton; step five, adding a proper amount of active carbon and diatomite into the solution obtained in the step four, uniformly stirring, filtering by a 0.45-micrometer filter membrane to remove impurities, degerming and decoloring to obtain filtrate; step six, concentrating the filtrate obtained in the step five under reduced pressure to 25-40% of the original volume to obtain a concentrated solution; and seventhly, pre-freezing the concentrated solution obtained in the sixth step at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of 1-10pa and-40 to-60 ℃ to obtain the deer bone marrow small molecule peptide freeze-dried powder, and carrying out low-temperature sealed storage at the temperature of-20 ℃.
Example 3
The deer bone marrow small molecule peptide freeze-dried powder composition is characterized by being combined according to parts by weight, the total weight is 100 parts, and the components are as follows: 50 parts of deer bone marrow small molecular peptide freeze-dried powder, 25 parts of deer skin collagen peptide freeze-dried powder, 10 parts of ginseng extract, 5 parts of angelica extract, 5 parts of longan extract and 5 parts of medlar extract, and the composition is prepared into capsules or tablets.
A preparation method of a deer bone marrow small molecule peptide freeze-dried powder composition comprises the following steps: step one, disinfecting and cleaning fresh deer bone marrow, and then quickly freezing to-18 to-40 ℃ for storage; step two, unfreezing and weighing the frozen deer bone marrow, adding deionized water which is 4-6 times of the weight of the unfrozen deer bone marrow, and homogenizing under the high pressure of 20-40 Mpa; step three, adding at least one of neutral protease, alkaline protease, papain and bromelain into the homogenized deer bone marrow solution for ultrasonic enzymolysis, wherein the enzymolysis conditions are as follows: adding enzyme at 4000U-6000U/g based on deer bone marrow, ultrasonic power of 500W, enzymolysis temperature of 50-60 deg.C, enzymolysis time of 4-6h, enzymolysis pH of 7-8, and enzyme-inactivating treatment at 100 deg.C for 10-20min to obtain deer bone marrow small molecule peptide solution; step four, refrigerating and storing the solution obtained in the step three for 24 hours, separating and removing upper fat, and filtering the solution through PP cotton; step five, adding a proper amount of active carbon and diatomite into the solution obtained in the step four, uniformly stirring, filtering by a 0.45-micrometer filter membrane to remove impurities, degerming and decoloring to obtain filtrate; step six, concentrating the filtrate obtained in the step five under reduced pressure to 25-40% of the original volume to obtain a concentrated solution; and seventhly, pre-freezing the concentrated solution obtained in the sixth step at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of 1-10pa and-40 to-60 ℃ to obtain the deer bone marrow small molecule peptide freeze-dried powder, and carrying out low-temperature sealed storage at the temperature of-20 ℃.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (2)
1. The deer bone marrow small molecule peptide freeze-dried powder composition is characterized by being combined according to parts by weight, the total weight is 100 parts, and the components are as follows: 40-50 parts of deer bone marrow small molecular peptide freeze-dried powder, 20-25 parts of deer skin collagen peptide freeze-dried powder, 5-10 parts of ginseng extract, 5-15 parts of angelica sinensis extract, 5-15 parts of longan extract and 5-15 parts of medlar extract, and the composition is prepared into capsules or tablets.
2. A preparation method of a deer bone marrow small molecule peptide freeze-dried powder composition comprises the following steps:
step one, disinfecting and cleaning fresh deer bone marrow, and then quickly freezing to-18 to-40 ℃ for storage;
step two, unfreezing and weighing the frozen deer bone marrow, adding deionized water which is 4-6 times of the weight of the unfrozen deer bone marrow, and homogenizing under the high pressure of 20-40 Mpa;
step three, adding at least one of neutral protease, alkaline protease, papain and bromelain into the homogenized deer bone marrow solution for ultrasonic enzymolysis, wherein the enzymolysis conditions are as follows: adding enzyme at 4000U-6000U/g based on deer bone marrow, ultrasonic power of 500W, enzymolysis temperature of 50-60 deg.C, enzymolysis time of 4-6h, enzymolysis pH of 7-8, and enzyme-inactivating treatment at 100 deg.C for 10-20min to obtain deer bone marrow small molecule peptide solution;
step four, refrigerating and storing the solution obtained in the step three for 24 hours, separating and removing upper fat, and filtering the solution through PP cotton;
step five, adding a proper amount of active carbon and diatomite into the solution obtained in the step four, uniformly stirring, filtering by a 0.45-micrometer filter membrane to remove impurities, degerming and decoloring to obtain filtrate;
step six, concentrating the filtrate obtained in the step five under reduced pressure to 25-40% of the original volume to obtain a concentrated solution;
and seventhly, pre-freezing the concentrated solution obtained in the sixth step at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of-40 to-60 ℃ under the condition of 1-10pa to obtain the deer bone marrow small molecule peptide freeze-dried powder, and carrying out low-temperature sealed storage at the temperature of-20 ℃.
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