CN102839206A - Preparation method for promoting self generation of human bone marrow hematopoietic stem cells - Google Patents
Preparation method for promoting self generation of human bone marrow hematopoietic stem cells Download PDFInfo
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- CN102839206A CN102839206A CN2012103405640A CN201210340564A CN102839206A CN 102839206 A CN102839206 A CN 102839206A CN 2012103405640 A CN2012103405640 A CN 2012103405640A CN 201210340564 A CN201210340564 A CN 201210340564A CN 102839206 A CN102839206 A CN 102839206A
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Abstract
The invention provides a preparation method for promoting generation of human bone marrow hematopoietic stem cells, and relates to a manufacture method of stem cells preparation. The method successively comprises the following steps of: a, collecting bovine placenta and fetal bovine marrow; b, cleaning, and sterilizing; c, separating protein, fat and active materials through a freezing centrifugal machine in an ultrafiltration method, so that the sediment is obtained; d, adding pepsase; e, adding water, and extracting to obtain fetal bovine marrow active polypeptide; f, getting the fetal bovine marrow active polypeptide, banana seedcase element and echinacea polysaccharide to be uniformly mixed to obtain marrow polysaccharide; g, getting the marrow polysaccharide, adding the water, and adding strontium, zinc, selenium, iodine, metasilicic acid, calcium, magnesium, potassium, sodium, iron, copper, lithium and nickel, and adjusting a pH value to be alkalescent, and then compounding to obtain a biological activity oral liquid preparation suitable for a human body. The preparation method has the beneficial effect that the human medullary hematopoiesis can be promoted to produce the hematopoietic stem cells; and an unique effect of treating various diseases can be achieved; and the preparation method is safe, efficient and has low cost, and prevention, health care and treatment effects can be achieved.
Description
Technical field
The present invention relates to life science and biological technical field, further is a kind of method of manufacture of stem cell medicine, especially people produce marrow hemopoietic stem cells from body liquid preparation method.
Background technology
Life science is one of the swiftest and the most violent subject of twentieth century development, has become the most noticeable field in the natural science.The discovery of HSCT technology and be applied as human Fighting Disease and bring new hope.
On May 14th, 2012, the Department of Science and Technology prints and distributes " great scientific research plan " the 12 " ad hoc planning of stem-cell research country " for (2012) No. 627 with state Ke Faji.
On November 3rd, 2009, Premier Wen Jiabao sets up to make a speech in 60 Annual General Meetings in the Chinese Academy of Sciences and points out: stem-cell research has promoted the development of regenerative medicine, and it is another medical revolution after pharmacological agent, operative treatment.We will strive obtaining the leading position in the more areas of stem-cell research, will pay much attention to simultaneously
The Chinese government pays much attention to stem-cell research.The Eleventh Five-Year Plan period; Fundamental research, gordian technique and the resource platform construction of stem cell supported in 973 plans, 863 Program and growth and the great scientific research plan of reproductive study country energetically, obtained a collection of marked achievement in stem-cell research and Transformation Application field: the totipotency that has proved mouse inductive pluripotent stem cells (iPS cell) in the world first; Disclose the initial molecular mechanism of reprogramming of somatic cells first; Successfully set up the inductive pluripotent stem cells of rat and pig first; Find the effective way of the transformation efficiency of raising inductive pluripotent stem cells; Identified the molecular marker of stem cell dryness etc.; A collection of therapeutic stem cell product and tissue engineering product have been researched and developed; Screen and studied a collection of micromolecular compound that can promote the stem cell self, improve iPS cell induction and raising stem cell directional differentiation efficiency; Having set up the human embryo stem cell (ES) that is fit to clinical application more is; Found the transcription factor of new regulation and control ES cell self-renewal.China significantly promotes in the international influence in stem-cell research field.
The father of stem cell---biomass cells scholar, the Wu ancestral of Chinese Academy of Sciences pool academician answer reporters' questions when setting up first stem cell bank in Henan Province: stem cell is one type of multipotential cell with the of self-replication capacity; Under certain condition, it can be divided into multiple pluripotent cell.Stem cells technology can be treated various diseases, and it will cause a medical science revolution.The disease that the conventional medicament treatment can't resolve, the stem cells technology treatment is a kind of good method.
At present, stem cell therapy adopts has four kinds: 1, autologous stem cells generates (marrow hemopoietic stem cells of promptly growing) on one's body; 2, adult's Bone Marrow Stem Cells Transplantation; 3, adult's tip hemocytoblast is transplanted; 4, cord blood stem cell is transplanted.
Existing stem cell transplantation technical disadvantages: two kinds of adult stems are transplanted, all can be because of mating the improper graft-rejection that occurs; Cord blood stem cell the possibility of rejection then occurs and wants much little.Adult's Bone Marrow Stem Cells Transplantation, adult's tip hemocytoblast are transplanted, cord blood stem cell is transplanted three kinds of stem cell transplantation costs up to hundreds of thousands of, and general audient holds economically and can't stand.
Summary of the invention
The purpose of this invention is to provide a kind of people of promotion produces marrow hemopoietic stem cells from body formulation method; It can promote the people to produce the generation (marrow hemopoietic stem cells of promptly growing) of marrow hemopoietic stem cells on one's body from body; Can rejection not take place, cost is low; Result of use is good, and the audient is easy to accept, safety, efficient.
The present invention adopts Bovine Placenta and tire Medulla Bovis seu Bubali to be made into tire Medulla Bovis seu Bubali active polypeptide; Any of several broadleaf plants skin is plain through adding, the echinacea purpurea polysaccharide is combined into the marrow polysaccharide, adds strontium, zinc, selenium, iodine, metasilicic acid, calcium, magnesium, potassium, sodium, iron, copper, lithium, bromine etc. again and processes biologically active agents, during use; Directly act on people's marrow; Make the people generate Red bone marrow from body, Red bone marrow hematopoiesis directly generates marrow hemopoietic stem cells.
The objective of the invention is to realize through following scheme:
A kind of formulation method that promotes the people from body generation marrow hemopoietic stem cells comprises and carries out following steps successively:
A is the ratio of 1-2:1 by weight, gathers Bovine Placenta and tire Medulla Bovis seu Bubali, and low temperature liquid nitrogen (the liquid nitrogen minimum temperature is subzero 209.86 degrees centigrade under the standard pressure) is preserved;
B carries out sterilization by the ozone ordinary method after pure water cleans;
C adopts refrigerated centrifuge, and (ultrafiltration process is a kind of membrane filtration with ultrafiltration process; It with porous membrane as separating medium; Rely on film pressure at both sides difference to come the material of different molecular weight in the separation solution as impellent, desalination is dirty contracts to the method for effects such as level, purification thereby play.It has the conversion that do not have phase, need not heating, consuming little energy, operational condition be gentle, needn't add chemical reagent, do not damage advantage such as temperature-sensitive medicine) carry out that albumen sepn, fat are separated, active substance separates; Wherein, the rotating speed of whizzer is more than 9000 rev/mins; 0.5-1 stop after hour; Obtain settling like this;
D adds 30 milligrams of pepsic ratios in every gram settling and adds stomach en-(content is more than 99%), under 35 ℃ of-40 ℃ of conditions, stops 100-120 minute; Adjustment pH value to 1.7;
E adds entry by weight the ratio of 6:1, extracting 4-5 hour; Obtain tire Medulla Bovis seu Bubali active polypeptide.(molecular mass is below 10000) (extraction yield of tire Medulla Bovis seu Bubali active polypeptide can reach more than 9.93%);
F gets above-mentioned tire Medulla Bovis seu Bubali active polypeptide, and any of several broadleaf plants skin plain (content is 200mg/10ml), echinacea purpurea polysaccharide (every content is 200mg/10ml) by weight the ratio of 1:1:1, mix and are combined into the marrow polysaccharide;
G gets marrow polysaccharide 15g; Add 1 liter in water; Add strontium 0.9-1.4mg, zinc 0.5-0.51mg, selenium 0.006-0.008mg, iodine 0.03-0.05mg, metasilicic acid 25-27mg, calcium 63-72mg, magnesium 17-20mg, potassium 2.36-3.70mg, sodium 54-57mg, iron 0.20-0.32mg, copper 0.01-0.02mg, lithium 0.5-0.86mg, bromine 0.1-0.2mg; Transfer pH value 7.2-7.8 weakly alkaline; The system converted in proportion in two hours mixes, and subzero 10 ℃ of-20 ℃ of mixing can generate the available biologically active oral liquid of human body--and promote the people to produce the preparation (oral liquid) of marrow hemopoietic stem cells from body.
Said tire Medulla Bovis seu Bubali active polypeptide, marrow polysaccharide and promotion people may be separately formed the production marketing use from the preparation (oral liquid) that body produces marrow hemopoietic stem cells.
Os Bovis seu Bubali ploypeptide can promote osteoblastic proliferation.Tire Medulla Bovis seu Bubali active polypeptide can rise medullary cell and repair and targeting.The tire Medulla Bovis seu Bubali is an embryonic stem cell, and embryonic stem cell is minimus stem cell, and marrow is the maximum hemopoietic stem cell of human body, and marrow is again the maximum hemocytopoietic organ of human body, and marrow is the brooder of all immunocytes.Tire Medulla Bovis seu Bubali active polypeptide contains growth factor and positive growth factor.Damage to gene DNA plays repair.Can make the nervus centralis reparative regeneration of damage, can make the organs and tissues of pathology rebuild function, can make that the marrow hemopoiesis function improves, epidermis is more living.Can impel the sexual gland secretion.The endocrine regulation balance can be replenished the anti-ageing anti-old factors such as thymosin.Improve hemopoietic stem cell quantity, promote hemocyte to upgrade and regeneration.Suppress cytopathy; Activating cells is active; Effectively eliminate the radical extremely harmful to human body; Repair the human body DC, improve cell metabolism; Promote, keep the cell eubolism.
Assistant with any of several broadleaf plants skin element increase that marrow, echinacea purpurea polysaccharide open that skeleton artery nutrient foramen, the strong bone of strontium green blood, zinc improve that immunizing power, selenium life special effect element are promoted longevity, to cause physiological function is normal, metasilicic acid constitutes the vessel wall staple, calcium is formed the skeleton tooth, the magnesium activating enzyme forms, potassium plays the nerve conduction effect, sodium is heartbeat propulsion source, iron be that oxyphorase important composition composition, copper are that micro elements needed by human, lithium increase neutrophil cell to the cns regulatory function and phagolysis enhancing, bromine are regulated neural function etc. and passed through rational proportion to the conversion of iodine start-up energy; Process the oral liquid of needed by human body; Be convenient to absorption of human body, directly act on marrow and make human body self produce marrow hemopoietic stem cells.
The invention has the beneficial effects as follows: modern scientific research finds, the stem cell nine big systemic diseases of can treating, repair, regenerate.Product of the present invention directly acts on marrow in human body; Can impel human bone marrow's hematopoiesis to produce hemopoietic stem cell; To treating multiple disease unique effect is arranged; Reached the many sick effects of medicine treatment, broken the tradition of how sick multiple medicines, and made the disease reverse of thinking incurable disease on the traditional medicine become possibility.Started regenerative medicine frontier science and technology summit.Safely, efficiently, at a low price, efficient 98.9%.Can be used for prevention, health care and treatment.
Below further prove the effect of product of the present invention through clinical testing data:
1, case is selected:
Select 181 routine people such as femoral head disease, tetter, hepatopathy, encephalopathic.Wherein, male 99 people, women 82 people, 9 examples below 20 years old wherein, 20～30 years old 18 example, 31～40 years old 29 example, 41～50 years old 39 example, 51～60 years old 41 example, 45 examples more than 60 years old.Course of disease elder 63 years, weak point person January, 3～90 days treatment times.
2, treat-ment:
During use, oral 3-6 time of every day, each 10-100 milliliter.Oral according to the course of treatment.3 months 1 courses of treatment.
3, contraindication:
In hematencephalon January, heart and brain bridging proping, did organ transplantation, implanted a pacemaker, wouldn't take.
4, efficacy assessment standard:
Cure: transference cure, function recover normal fully or basically, and the viability of resuming work is observed efficacy consolidation person half a year.
Produce effects: former symptom is most of to be eliminated, and function is most of to be recovered, and part is resumed work and viability.
Effectively: former symptom is partly improved, and funtion part is recovered.
Invalid: former symptom no change or do not have considerable change.
5, result of treatment:
1. treat necrosis of femoral head 56 examples, cure 35 examples, produce effects 11 examples, effective 9 examples, invalid 1 example, total effective rate 98.2%; 2. treat vitiligo, psoriasis 44 examples, cure 27 examples, produce effects 11 examples, effective 6 examples, total effective rate 100%; 3. treat liver cirrhosis 50 examples, cure 29 examples, produce effects 14 examples, effective 6 examples, invalid 1 example, total effective rate 98%; 4. treat brain paralysis cerebral infarction 13 examples, cure 5 examples, produce effects 6 examples, effective 1 example, invalid 1 routine total effective rate 87%; 5. treat other disease 18 examples, cure 11 examples, produce effects 4 examples, effective 3 examples, total effective rate 100%; Total treatment 181 examples, total effective rate 98.9%.
Explain: cure rapidly or produce effects flu, diarrhoea etc. are arranged.Indivedual invalid examples are because treatment is untimely, or treatment time is short, or sickly plant too much, or taking dose is too for a short time causes, and stem cell cleaning radical needs the time, and theoretically, product of the present invention is should 100% effective.
6, part model case:
Case 1: Wuhan Chen, woman, 63 years old; In May, 2011 ovarian cancer ascites postoperative; Four tumor markerses exceed standard, June chemotherapy once, it is longer again as young girl's breast to obey product rear udder attachment of the present invention July; The people seems, and spirit is young, inspection each item index is normal, and each side in order.
Case 2: Zhang: the man, 56 years old, people from Zhengzhou City, Henan Province, downright bad 8 years post crutches of bilateral femoral head are close to paralysis, and obey product of the present invention in June, 2011, after three months courses of treatment, throws away crutches and return on foot and do not rock, and can top bar step by step.Same normal people at present walks.Young ten years old.
Case 3: Liu so-and-so, the woman, 56 years old, people from Gongyi City, Henan Province, whole body distribution vitiligo 6 years, myocardial ischemia is often fallen in a swoon on foot, trick decortication, thyromegaly.With oral product of the present invention 3 months, Tiroidina was normal basically, obeys 5 months, and vitiligo can't see basically on the both hands, during do not fall in a swoon again on foot, trick is no longer peeled, and obeys 7 months, whole body and belly vitiligo recover normal basically.
Case 4: close so-and-so, man, 56 years old, people from Zhengzhou City, Henan Province, psoriasis, crown alopecia, prostatitis, presbyopia.Obey one week of product of the present invention, upstairs leg joint is not painful, and asthenopia has been got well; The clothes first quarter moon, the prostatitis symptom disappears; Behind one wheat harvesting period, the blackening of right back brain white hair; Take half a year, psoriasis has been got well on the leg, obeyed 1 year, and hair generating, wrinkle disappears on the face, and is glowing with health.
Case 5: Lee so-and-so, the woman, 38 years old, the flat-top recluse of Henan Province suffered from systemic lupus erythematous two-and-a-half years, weak.Zhengzhou, Shanghai, Beijing Duo Jia large hospital treatment cost more than 30 ten thousand are controlled heavier and heavier, leave hospital and walk not Cheng Lu; The whole body arthritis because of increasing to more than 160 jin with the hormone body weight by 123 jin in hospital, can not moved on the bed of lying; Can adept machine, need 3 people to serve sometimes, go home to window and be afraid of flu; 4 plus siges of urine protein, the immunizing power extreme difference.Alleviate with oral product one all arthritis of the present invention; It is not painful to obey 2 months joints, and urine protein is turned out cloudy; Obeyed 6 months, the inspection of each item index is normal, and body weight is reduced to 138 jin.
Case 6: Yang, woman, 50 years old, people from Shiyan City, Hubei Province, liver cirrhosis.Spend several ten thousand yuan every year in hospital, and the state of an illness progressively deteriorates to and can not have a meal, and subsists by eating fruit gruel, lean and haggard, worn to a shadow, obeys three first quarter moons of product of the present invention, and normal basically to examination in hospital liver index, face has been had a good complexion, and can have a meal, and whole body is strong.
Case 7: the field so-and-so, the woman, 40 years old, people from Zhengzhou City, Henan Province, blood transfusion causes third liver when giving birth to daughter, hides general weakness 17 years.Multi-treatment is invalid, obey product of the present invention after whole body strong, tinea unguium has been got well, inspection each item index is normal.
Case 8: Liu so-and-so, the woman, 21 years old, people from Zhengzhou City, Henan Province suffered from tuberculous meningitis in October, 2011, the back in hernia cerebri, hydrocephalus, blindness.The whole body jaundice is sprayed Rifampin and is destroyed liver, and amenorrhoea always, is had a meal fewly at poor appetite, and the people becomes thin, and the right eye brow ridge is innately outstanding, and invalid in hospital, twelfth month 23 left hospital.Clothes on May 5th, 2012 product of the present invention.May 22 meeting: can have a meal, passwind many, superly sleep, also sleep daytime, former energetically eyes can be seen also upper and lower the 6th buildings, tower rank than eating, oneself is out of question on foot; Right eye brow ridge protuberance dwindles, and the mental status is clearly better.Evening May 30 is in the period; August 2 weighed, and body weight increases by 5 kilograms before obeying this product.
Case 9: Zhan, the man, 68 years old, people from Zhengzhou City, Henan Province, the big area cerebral embolism, brain, brain stem, thalamus thrombus, the multiple cerebral infarction of the right hemisphere of cerebellum, 1963 trachitis, metopantritis.49 years trachitis has been got well after obeying product of the present invention, and metopantritis has been got well, and is radiant with vigour, 80% newborn Hei Fa, and it is in high spirits as the youngster to walk.
Case 10: Peng, the man, 27 years old, people from Wuhan City, Hubei Province, brain paralysis, right leg is disabled, stutter.On September 6th, 2011 is with oral product of the present invention.Not lame can normally walking of right leg on March 24th, 2012, mouth is fulled up normal.
Case 11: Liu, man, 76 years old, people from Gongyi City, Henan Province, stroke hemiplegia 6 years.Obey product one and a half months of the present invention, do not slobber, leg can be lifted, and urine does not drip.
Case 12: Zhang, man, 41 years old, the Jiaozuo City people of Henan Province, carbon monoxide poisoning plant person in 19 years old.The treatment back is sociable, and trick is not taken out and can be sat, and the clothes hemopoietic stem cell can be sat in two months, and hand can be grabbed chair by stiff deliquescing.
Case 13: Zhang, man, 48 years old, people from Gongyi City, Henan Province, parkinson 6 years.Obeying 3 months hands of product of the present invention can write, and can hold vermicelli under the arm with chopsticks, continues to take.
Case 14: revive so-and-so, the man, 36 years old, people from Zhengzhou City, Henan Province, Zhengzhou City hospital is lived in April, 2010 unexpected headache, vomiting, is diagnosed as hypertension, hernia cerebri, and the first quarter moon of being in hospital spends nearly 200,000 yuan.Even if the doctor says that leaving hospital also is the plant person, is in peril of one's life in the first quarter moon.Leave hospital back in November, 2010 with oral product of the present invention, opened eyes in 3 days, a wheat harvesting period pin has consciousness; Obeyed 5 months, and can walk by sustaining wall, can stand one hour, sociable.
Case 15: Cai so-and-so, woman, 38 years old, people from Xinmi City, Henan Province, white blood disease.Obeyed product of the present invention 4 months, it is normal to look into red corpuscle, oxyphorase, white corpuscle, thrombocyte four indices.Continue to take firm.
Case 16: the hole so-and-so, the man, 52 years old, people from Jiyuan City, Henan Province, white blood disease, lymphatic cancer with oral product of the present invention month, was obeyed 40 days, neck, chest, upper body many places tumour disappear.
Case 17: go into business so-and-so, woman, 65 years old, the flat-top recluse of Henan Province, spinal canal stenosis 8 years; Both legs amyotrophy, shank have only bone not have muscle, and 90 degree are bent over, and paralysis can not be walked; Can't take care of oneself, obey two months longue meat of product of the present invention, waist straightened up in four months, recovery from illness in five months.Can walk on their own, can look after children and cook.
Case 18: Huang: the man, 10 years old, people from Zhengzhou City, Henan Province, daily inspection in July 31 in 2006 is progressive muscular dystrophy.In many ways it is invalid to seek medical advice.In July, 2010, nerve was dredged behind two first quarter moons with oral product of the present invention.Look into the creatine creatase in March, 2011 and drop to 2777 from 14654.Body weight rises to 78 jin from 32 jin in half a year.Understand and oneself get up, sit, stand and walk, can play computer, spirit is good, and intelligence is normal.
Case 19: high so-and-so, the man, 42 years old, people from Urumqi City, Xinjiang, mellitus, fasting plasma glucose 29mmol/l, alopecia, prostatomegaly, uroschesis is put catheter 2 wheat harvesting periods, can not walk, and can only move the step.The two famous hospitals in Beijing, two experts that enjoy State Council's subsidy point out to do fistulization and change operation on urethra, have nothing for it.After took product of the present invention 35 days, sexual function is arranged, urine meaning is arranged, pull out catheter, blood sugar descends, and makes a call in one day 3 times by originally spraying 45 units of Regular Insulin, stops to beat Regular Insulin by reducing to, blood sugar drops to the 6-7 point, newly blackly goes out, on foot normally.
Case 20: Guo so-and-so, man, 75 years old, people from Zhengzhou City, Henan Province, sacroiliitis, cervical spondylosis, hemorrhoid, full white hair, constipation.One course of treatment of on January 8th, 2011 clothes product of the present invention.The presbyopia has got well; Prostatitis has been got well; It is normal that sexual function improving recovers; Wrinkle disappears; May 10 face by original pitch-dark become ruddy flexible glossy; Gonitis had been got well in more than 20 year; A change Sheng Heifa covered with silver hair! Hemorrhoid have been got well, and cervical vertebra has been got well, and eyes are dry and astringent good, and spleen, diarrhoea constipation have been got well.Senile plaque disappears.
Embodiment
In order to make those skilled in the art person understand the present invention program better, and make above-mentioned purpose of the present invention, feature and advantage can be more obviously understandable, below in conjunction with embodiment the present invention done further detailed explanation.
Embodiment 1:
Formulation method from body generation marrow hemopoietic stem cells comprises and carries out following steps successively:
A is the ratio of 1:1 by weight, gathers Bovine Placenta and tire Medulla Bovis seu Bubali, and liquid nitrogen cryogenics is preserved;
B carries out sterilization by the ozone ordinary method after pure water cleans;
C adopts refrigerated centrifuge to carry out albumen sepn, fat separation, active substance separation with ultrafiltration process, and wherein, the rotating speed of whizzer is 9100 rev/mins; 0.5 stop after hour; Obtain settling like this,
D adds 30 milligrams of pepsic ratios in every gram settling and adds stomach en-(content is more than 99%), under 35 ℃ of conditions, stops 100 minutes; Adjustment pH value to 1.7;
E adds entry by weight the ratio of 6:1, extracting 4 hours; Obtain tire Medulla Bovis seu Bubali active polypeptide;
F gets above-mentioned tire Medulla Bovis seu Bubali active polypeptide, and any of several broadleaf plants skin plain (content is 200mg/10ml), echinacea purpurea polysaccharide (every content is 200mg/10ml) by weight the ratio of 1:1:1, mix and are combined into the marrow polysaccharide;
G gets marrow polysaccharide 15g; Add 1 liter in water; Add strontium 0.9mg, zinc 0.5mg, selenium 0.006mg, iodine 0.03mg, metasilicic acid 25mg, calcium 63mg, magnesium 17mg, potassium 2.36mg, sodium 54mg, iron 0.2mg, copper 0.01mg, lithium 0.5mg, bromine 0.1mg; Transfer pH value 7.2 weakly alkalines, the system converted in proportion in two hours mixes the available biologically active oral liquid body preparation of subzero 10 ℃ of synthesized humans.
Embodiment 2:
Formulation method from body generation marrow hemopoietic stem cells comprises and carries out following steps successively:
A is the ratio of 2:1 by weight, gathers Bovine Placenta and tire Medulla Bovis seu Bubali, and liquid nitrogen cryogenics is preserved;
B carries out sterilization by the ozone ordinary method after pure water cleans;
C adopts refrigerated centrifuge to carry out albumen sepn, fat separation, active substance separation with ultrafiltration process, and wherein, the rotating speed of whizzer is 9000 rev/mins; Stop after 1 hour; Obtain settling like this,
D adds 30 milligrams of pepsic ratios in every gram settling and adds stomach en-(content is more than 99%), under 40 ℃ of conditions, stops 120 minutes; Adjustment pH value to 1.7;
E adds entry by weight the ratio of 6:1, extracting 5 hours; Obtain tire Medulla Bovis seu Bubali active polypeptide;
F gets above-mentioned tire Medulla Bovis seu Bubali active polypeptide, and any of several broadleaf plants skin plain (content is 200mg/10ml), echinacea purpurea polysaccharide (every content is 200mg/10ml) by weight the ratio of 1:1:1, mix and are combined into the marrow polysaccharide;
G gets marrow polysaccharide 15g; Add 1 liter in water; Add strontium 1.4mg, zinc 0.51mg, selenium 0.008mg, iodine 0.05mg, metasilicic acid 27mg, calcium 72mg, magnesium 20mg, potassium 3.70mg, sodium 57mg, iron 0.32mg, copper 0.02mg, lithium 0.86mg, bromine 0.2mg; Transfer pH value 7.8 weakly alkalines, the system converted in proportion in two hours mixes 20 ℃ of available biologically active oral liquid body preparations of synthesized human.
Embodiment 3:
Formulation method from body generation marrow hemopoietic stem cells comprises and carries out following steps successively:
A is the ratio of 1.5:1 by weight, gathers Bovine Placenta and tire Medulla Bovis seu Bubali, and liquid nitrogen cryogenics is preserved;
B carries out sterilization by the ozone ordinary method after pure water cleans;
C adopts refrigerated centrifuge to carry out albumen sepn, fat separation, active substance separation with ultrafiltration process, and wherein, the rotating speed of whizzer is more than 9000 rev/mins; 0.8 stop after hour; Obtain settling like this;
D adds 30 milligrams of pepsic ratios in every gram settling and adds stomach en-(content is more than 99%), under 38 ℃ of conditions, stops 110 minutes; Adjustment pH value to 1.7;
E adds entry by weight the ratio of 6:1, extracting 4-5 hour; Obtain tire Medulla Bovis seu Bubali active polypeptide;
F gets above-mentioned tire Medulla Bovis seu Bubali active polypeptide, and any of several broadleaf plants skin plain (content is 200mg/10ml), echinacea purpurea polysaccharide (every content is 200mg/10ml) by weight the ratio of 1:1:1, mix and are combined into the marrow polysaccharide;
G gets marrow polysaccharide 15g; Add 1 liter in water; Add strontium 1mg, zinc 0.5mg, selenium 0.007mg, iodine 0.04mg, metasilicic acid 26mg, calcium 68mg, magnesium 18mg, potassium 3mg, sodium 55mg, iron 0.26mg, copper 0.01mg, lithium 0.7mg, bromine 0.15mg; Transfer pH value 7.2-7.8 weakly alkaline, the system converted in proportion in two hours mixes subzero 10 ℃-20 ℃ available biologically active oral liquid body preparations of synthesized human.
The above; Be merely embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technician who is familiar with the present technique field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (6)
1. the making method of a tire Medulla Bovis seu Bubali active polypeptide is characterized in that comprising and carries out following steps successively:
A is the ratio of 1-2:1 by weight, gathers Bovine Placenta and tire Medulla Bovis seu Bubali, and liquid nitrogen cryogenics is preserved;
B carries out sterilization by the ozone ordinary method after pure water cleans;
C adopts refrigerated centrifuge to carry out albumen sepn, fat separation, active substance separation with ultrafiltration process, and wherein, the rotating speed of whizzer is more than 9000 rev/mins; 0.5-1 stop after hour; Obtain settling like this;
D adds 30 milligrams of pepsic ratios in every gram settling and adds stomach en-, under 35 ℃ of-40 ℃ of conditions, stops 100-120 minute; Adjustment pH value to 1.7;
E adds entry by weight the ratio of 6:1, extracting 4-5 hour; Obtain tire Medulla Bovis seu Bubali active polypeptide.
2. the making method of tire Medulla Bovis seu Bubali active polypeptide according to claim 1 is characterized in that: said pepsin content is more than 99%.
3. the making method of a marrow polysaccharide is characterized in that comprising and carries out following steps successively:
A is the ratio of 1-2:1 by weight, gathers Bovine Placenta and tire Medulla Bovis seu Bubali, and liquid nitrogen cryogenics is preserved;
B carries out sterilization by the ozone ordinary method after pure water cleans;
C adopts refrigerated centrifuge to carry out albumen sepn, fat separation, active substance separation with ultrafiltration process, and wherein, the rotating speed of whizzer is more than 9000 rev/mins; 0.5-1 stop after hour; Obtain settling like this;
D adds 30 milligrams of pepsic ratios in every gram settling and adds stomach en-, under 35 ℃ of-40 ℃ of conditions, stops 100-120 minute; Adjustment pH value to 1.7;
E adds entry by weight the ratio of 6:1, extracting 4-5 hour; Obtain tire Medulla Bovis seu Bubali active polypeptide;
F gets above-mentioned tire Medulla Bovis seu Bubali active polypeptide, and any of several broadleaf plants skin is plain, the echinacea purpurea polysaccharide, by weight the ratio of 1:1:1, mixes and is combined into the marrow polysaccharide.
4. the making method of marrow polysaccharide according to claim 3 is characterized in that: said pepsin content is more than 99%, and the plain content of said any of several broadleaf plants skin is 200mg/10ml, and every content of echinacea purpurea polysaccharide is 200mg/10ml.
5. one kind promotes the people to produce the formulation method of marrow hemopoietic stem cells from body, it is characterized in that comprising and carries out following steps successively:
A is the ratio of 1-2:1 by weight, gathers Bovine Placenta and tire Medulla Bovis seu Bubali, and liquid nitrogen cryogenics is preserved;
B carries out sterilization by the ozone ordinary method after pure water cleans;
C adopts refrigerated centrifuge to carry out albumen sepn, fat separation, active substance separation with ultrafiltration process, and wherein, the rotating speed of whizzer is more than 9000 rev/mins; 0.5-1 stop after hour; Obtain settling like this;
D adds 30 milligrams of pepsic ratios in every gram settling and adds stomach en-, under 35 ℃ of-40 ℃ of conditions, stops 100-120 minute; Adjustment pH value to 1.7;
E adds entry by weight the ratio of 6:1, extracting 4-5 hour; Obtain tire Medulla Bovis seu Bubali active polypeptide;
F gets above-mentioned tire Medulla Bovis seu Bubali active polypeptide, and any of several broadleaf plants skin is plain, the echinacea purpurea polysaccharide, by weight the ratio of 1:1:1, mixes and is combined into the marrow polysaccharide;
G gets marrow polysaccharide 15g; Add 1 liter in water; Add strontium 0.9-1.4mg, zinc 0.5-0.51mg, selenium 0.006-0.008mg, iodine 0.03-0.05mg, metasilicic acid 25-27mg, calcium 63-72mg, magnesium 17-20mg, potassium 2.36-3.70mg, sodium 54-57mg, iron 0.20-0.32mg, copper 0.01-0.02mg, lithium 0.5-0.86mg, bromine 0.1-0.2mg; Transfer pH value 7.2-7.8 weakly alkaline; The system converted in proportion in two hours mixes, and subzero 10 ℃ of-20 ℃ of mixing can generate the available biologically active oral liquid of human body--and promote the people to produce the oral liquid of marrow hemopoietic stem cells from body.
6. promotion people according to claim 5 is from the formulation method of body generation marrow hemopoietic stem cells, and it is characterized in that: said pepsin content is more than 99%, and the plain content of said any of several broadleaf plants skin is 200mg/10ml, and every content of echinacea purpurea polysaccharide is 200mg/10ml.
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