CN1537951A - Method of making deer dry cell protein bioactive poptide using microoprganism fermentation technology - Google Patents
Method of making deer dry cell protein bioactive poptide using microoprganism fermentation technology Download PDFInfo
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- CN1537951A CN1537951A CNA2003101076300A CN200310107630A CN1537951A CN 1537951 A CN1537951 A CN 1537951A CN A2003101076300 A CNA2003101076300 A CN A2003101076300A CN 200310107630 A CN200310107630 A CN 200310107630A CN 1537951 A CN1537951 A CN 1537951A
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Abstract
A process for preparing the active peptide of deer stem cell protein, which can be used to prepare medicine and health-care food, includes such steps as taking the bone marrow stem cells and thymus gland from artificially raise deer, special antistaling treating and deep microbial fermenting. Said active peptide can be used to regulate immunity, delay sanility, relieve fatigue and lower blood pressure.
Description
Affiliated field: the present invention relates to a kind of method that adopts microbial fermentation technology to make deer stem cell protein active peptide.
Background technology: also not having bone marrow stem cell, thymus gland with artificial breeding deer (red deer and spotted deer) both at home and abroad through update search at present is raw material, produce the method for stem cell active polypeptide, also find record for the bone marrow stem cell of artificial breeding deer (red deer and spotted deer), other utilization of thymus gland.Bone marrow stem cell, thymus gland are a kind of raw material and resources of uniqueness, and its product has active function, can be applicable to plurality kinds of health care products such as pharmacy, protective foods, pulvis, concentrated solution, oral liquid, can also make tablet or capsule with Chinese Medicine and Clavicular.
Summary of the invention: the purpose of this invention is to provide a kind of active function that has, and have better nutritivity to be worth, the employing microbial fermentation technology that absorbs is made the method for deer stem cell protein active peptide easily.The objective of the invention is to realize in the following manner: the present invention is a kind of method that adopts microbial fermentation technology to make deer stem cell protein active peptide, A: strain improvement lactobacillus A306 number, 36 ℃ of leavening temperatures, fermented 72 hours, raw material: red deer, spotted deer that plant oneself cultivates, with its bone marrow stem cell and Zadaxin is raw material, produces deer stem cell protein polypeptide; B: deer stem cell Preservation Treatment: handle with 5% Peracetic Acid and 70% alcohol respectively, it is fresh-keeping to be stored in-10 ℃ of refrigeration chambers with thermal protective aid again; C: raw material pulverizing: be crushed to the 80-100 order with cutter formula pulverizer, use the colloidal mill porphyrize again, sterilising treatment is 121 ℃ of high temperature moments; D: fermentation: adopt the stainless cylinder of steel submerged fermentation of system control, 36 ℃ of fermentation temperature, are handled through instant sterilizer after fermentation is finished at 72 hours time again; E: filter: adopt diatomite cardboard filter machine to carry out filter just, again through supercentrifuge 9000-10000 rev/min processing, and then through membrane filtration; F: vacuum concentration: use the stainless-steel vacuum thickening equipment, vacuum is 300 mercury column, and 60 ℃ of temperature guarantee that active substance is not damaged; G: cryogenic temperature freezing drying: adopt-40 ℃ of cryogenic temperature freezing drying machines, the cycle is 20 hours, makes deer stem cell protein peptide freezing dry powder; H: after dry powder was made, bioactive peptide content was 70%, in the operation of sterile packed chamber, with aluminium foil bag 5Kg capacity filling and sealing.The present invention finishes on the analysis basis of deer stem cell, the stability test of undergo microbial fermentation test, polypeptide, produces the product sample of stabilization process, stem cell degree of hydrolysis 80%, stem cell protein active peptide yield 70%.Comprise through experimentation on animals: toxicity, function, biological experiment etc. show: the product that the present invention produces has function such as regulate immune, anti-ageing, antifatigue, bring high blood pressure down, and is with a wide range of applications.Technical characterstic of the present invention is: artificial breeding red deer and spotted deer, using its bone marrow stem cell and thymus gland is raw material, fresh-keeping through special processing, adopts free bacterial strain to carry out the microorganism submerged fermentation, produces the active polypeptide of stem cell.Its product has active function, can be applicable to pharmacy protective foods, pulvis, concentrated solution, oral liquid and Chinese Medicine and Clavicular and makes tablet or capsule.Adopt compound prescription, strengthen function, produce originality healthcare products and the medicine domestic, that do not have as yet that show unique characteristics abroad.The present invention has novelty through patent retrieval, does not appear in the newspapers both at home and abroad, belongs to the project of plugging a gap.The present invention adopts the microbial technique fermentation to produce the bioactive peptide of deer stem cell, and this material has high nutrition, high reactivity and functional performance, belongs to domestic original creation technology.Finished product is produced and is adopted advanced cryogenic temperature freezing drying equipment, effectively protects activity and functional mass in the raw material, good product quality.Deer stem cell protein active peptide can combine with northern medicine and make compound formulation, can reach enhancement function, fully difference and the peptide made from other raw material.
Embodiment:
The present invention is a kind of method that adopts microbial fermentation technology to make deer stem cell protein active peptide, A: strain improvement lactobacillus A306 number, 36 ℃ of leavening temperatures, fermented 72 hours, raw material: red deer, spotted deer that plant oneself cultivates, with its bone marrow stem cell and Zadaxin is raw material, produces deer stem cell protein polypeptide; B: deer stem cell Preservation Treatment: handle with 5% Peracetic Acid and 70% alcohol respectively, it is fresh-keeping to be stored in-10 ℃ of refrigeration chambers with thermal protective aid again; C: raw material pulverizing: be crushed to the 80-100 order with cutter formula pulverizer, use the colloidal mill porphyrize again, sterilising treatment is 121 ℃ of high temperature moments; D: fermentation: adopt the stainless cylinder of steel submerged fermentation of system control, 36 ℃ of fermentation temperature, are handled through instant sterilizer after fermentation is finished at 72 hours time again; E: filter: adopt diatomite cardboard filter machine to carry out filter just, again through supercentrifuge 9000-10000 rev/min processing, and then through membrane filtration; F: vacuum concentration: use the stainless-steel vacuum thickening equipment, vacuum is 300 mercury column, and 60 ℃ of temperature guarantee that active substance is not damaged; G: cryogenic temperature freezing drying: adopt-40 ℃ of cryogenic temperature freezing drying machines, the cycle is 20 hours, makes deer stem cell protein peptide freezing dry powder; H: after dry powder was made, bioactive peptide content was 70%, in the operation of sterile packed chamber, with aluminium foil bag 5Kg capacity filling and sealing.
Applicating example of the present invention:
Deer spirit health electuary: A formula for a product (200 bags of every 1.5g, 2 bags/people/day)
Deer bone marrow stem cell protein peptide powder 30g
Radix Ginseng extract (quite genseng 100g) 20g
Radix Et Caulis Acanthopanacis Senticosi cream 0.5g
Zinc Gluconate 5g
Sodium Selenite 5.5mg
Rock sugar 75g
Amylodextrin adds to total amount 300g
This prescription is by road bone marrow stem cell protein peptide, and rare Chinese medicine and nutritive substances such as genseng, Radix Et Caulis Acanthopanacis Senticosi, Zinc Gluconate, Sodium Selenite are through scientific approach and the refining protective foods that forms of advanced technologies.Through functional experiment proof this product antifatigue and the nourishing function that delays senility are arranged.
Claims (1)
1, a kind of preparation method who adopts microbial fermentation technology to make the method for deer stem cell protein active peptide, it is characterized in that: A: strain improvement lactobacillus A306 number, 36 ℃ of leavening temperatures, fermented 72 hours, raw material: red deer, spotted deer that plant oneself cultivates, with its bone marrow stem cell and Zadaxin is raw material, produces deer stem cell protein polypeptide; B: deer stem cell Preservation Treatment: handle with 5% Peracetic Acid and 70% alcohol respectively, it is fresh-keeping to be stored in-10 ℃ of refrigeration chambers with thermal protective aid again; C: raw material pulverizing: be crushed to the 80-100 order with cutter formula pulverizer, use the colloidal mill porphyrize again, sterilising treatment is 121 ℃ of high temperature moments; D: fermentation: adopt the stainless cylinder of steel submerged fermentation of system control, 36 ℃ of fermentation temperature, are handled through instant sterilizer after fermentation is finished at 72 hours time again; E: filter: adopt diatomite cardboard filter machine to carry out filter just, again through supercentrifuge 900-10000 rev/min processing, and then through membrane filtration; F: vacuum concentration: use the stainless-steel vacuum thickening equipment, vacuum is 300 mercury column, and 60 ℃ of temperature guarantee that active substance is not damaged; G: cryogenic temperature freezing drying: adopt-40 ℃ of cryogenic temperature freezing drying machines, the cycle is 20 hours, makes deer stem cell protein peptide freezing dry powder; H: after dry powder was made, bioactive peptide content was 70%, in the operation of sterile packed chamber, with aluminium foil bag 5Kg capacity filling and sealing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101076300A CN1537951A (en) | 2003-10-23 | 2003-10-23 | Method of making deer dry cell protein bioactive poptide using microoprganism fermentation technology |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101076300A CN1537951A (en) | 2003-10-23 | 2003-10-23 | Method of making deer dry cell protein bioactive poptide using microoprganism fermentation technology |
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| CN1537951A true CN1537951A (en) | 2004-10-20 |
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| CNA2003101076300A Pending CN1537951A (en) | 2003-10-23 | 2003-10-23 | Method of making deer dry cell protein bioactive poptide using microoprganism fermentation technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104894194A (en) * | 2015-06-19 | 2015-09-09 | 广西复鑫益生物科技有限公司平南分公司 | Production method of leech stem cell protein active peptide |
| CN112515177A (en) * | 2020-12-24 | 2021-03-19 | 吉林省东鳌鹿业科技开发有限公司 | Deer bone marrow small molecule peptide freeze-dried powder composition and preparation method thereof |
-
2003
- 2003-10-23 CN CNA2003101076300A patent/CN1537951A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104894194A (en) * | 2015-06-19 | 2015-09-09 | 广西复鑫益生物科技有限公司平南分公司 | Production method of leech stem cell protein active peptide |
| CN112515177A (en) * | 2020-12-24 | 2021-03-19 | 吉林省东鳌鹿业科技开发有限公司 | Deer bone marrow small molecule peptide freeze-dried powder composition and preparation method thereof |
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