CN109350709A - A kind of herb mixture and preparation method thereof of the anti-curing oncoma of hypoglycemic - Google Patents
A kind of herb mixture and preparation method thereof of the anti-curing oncoma of hypoglycemic Download PDFInfo
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Abstract
The present invention provides a kind of drugs of the anti-curing oncoma of hypoglycemic, including 3-15 parts of ginseng, 5-20 parts of thallus laminariae, 5-25 parts of fructus lycii, 4-16 parts of mulberry leaf, 10-30 parts of semen coicis, 4-20 parts of cape jasmine, 30-160 parts of agaricus bisporus, 0.00001-0.002 parts of selenium, 0.00005-0.002 parts of zinc, 0.00002-0.001 parts of chromium.Drug of the present invention be on the basis of thallus laminariae semen coicis increase ginseng, fructus lycii, can help healthy tendency, improve viscera function, enhance the immune function and mobility of human body；Increase mulberry leaf cape jasmine, can activating blood, resolving stasis, dispelling its stasis of blood it is stagnant；Increase edible fungus mushroom, selenium, chromium, zinc, achievees the effect that hypoglycemic antitumor curative effect synthesis improves and toxic side effect is not superimposed, therapeutic index improves 30% or more.
Description
Technical field
The invention belongs to field of medicaments, relate generally to a kind of drug and preparation method thereof of anti-curing oncoma of hypoglycemic, tool
Body be it is a kind of with hypoglycemic, reducing blood lipid, anti-tumor function and microbial fermentation technology preparation Chinese medicinal food mixture and its
Preparation method.
Background technique
Tumour and diabetes and concurrent cardiovascular and cerebrovascular disease are the great refractory diseases for threatening human life and health, in people
It experienced very long historical upheaval in the therapeutic process of class.Chinese medicine is important one for the treatment of means, with the depth of research
Enter the development with science and technology, drug is constantly progressive with the discovery of novel targets.And scientist sends out the further investigation of tumour
It is existing, it is all extremely closely related with blood pressure and blood lipoid after all although the reason of tumour occurrence and development is sufficiently complex.Meanwhile
Hyperglycemia and tumour are deeline, and hypoimmunity, easily occur together severe infections and infection symptoms relatively concealment, are sent out repeatedly
It is raw not easy to control, the two and when depositing interaction aggravate the state of an illness, poor prognosis.Due to abnormal gene expression be formed by it is primary and after
Deterioration of the tumour ambient enviroment of hair, such as angiogenesis, function of immune system variation abnormality etc., hyperglycemia hyperlipidemia promote swollen
The pernicious apparent further development of oncocyte, and generate diffusion in vivo and transfer.
Chinese medicine thinks that the origin cause of formation of tumour has " healthy tendency is deficient, the invasion of zang-fu disharmony, exopathogen " etc., and blood pressure and blood lipoid is abnormal, most
End form forms tumour at phlegm, the stasis of blood, poison, by this three cohesion.Traditional Chinese medicine and pharmacy theory thinks that most of tumor patient constitution is weaker.
Thus, this invention address that providing that a kind of enhancing immune function of human body, activating microcirculation and removing stasis medicinal dissolving stasis is stagnant, hypoglycemic antitumor curative effect is comprehensive
It closes and improves and herb mixture and preparation method thereof that toxic side effect is not superimposed.
Summary of the invention
The object of the present invention is to provide a kind of therapeutic effect is good and the Chinese medicine for the anti-curing oncoma of hypoglycemic that toxic side effect is low
Mixture and preparation method thereof.
The present invention realizes that above-mentioned purpose is adopted the technical scheme that, prepares drug by the following raw material.
A kind of herb mixture of the anti-curing oncoma of hypoglycemic, made of the raw material of following weight proportion:
3-15 parts of ginseng, 5-20 parts of thallus laminariae, 5-25 parts of fructus lycii, 4-16 parts of mulberry leaf, 10-30 parts of semen coicis, cape jasmine 4-20
Part, 30-160 parts of agaricus bisporus, 0.00001-0.002 parts of selenium, 0.00005-0.002 parts of zinc, 0.00002-0.001 parts of chromium.
Further preferably, herb mixture of the present invention is made of the raw material of following weight proportion:
3-8 parts of ginseng, 5-12 parts of thallus laminariae, 8-15 parts of fructus lycii, 5-10 parts of mulberry leaf, 15-20 parts of semen coicis, cape jasmine 4-10
Part, 100-160 parts of agaricus bisporus, 0.0001-0.001 parts of selenium, 0.0001-0.001 parts of zinc, 0.0001-0.001 parts of chromium.
Most preferably, herb mixture formula best weight ratio of the present invention is:
3 parts of ginseng, 9 parts of thallus laminariae, 10 parts of fructus lycii, 8 parts of mulberry leaf, 15 parts of semen coicis, 8 parts of cape jasmine, 150 parts of agaricus bisporus, selenium
0.0006 part, 0.0009 part of zinc, 0.0004 part of chromium.
The present invention also provides the preparation methods of the herb mixture described in one kind, comprising the following steps:
(1) chromium powder, agaricus bisporus are prepared into chromium-rich agaricus bisporus；
(2) thallus laminariae, semen coicis, fructus lycii, mulberry leaf, ginseng, cape jasmine and chromium-rich agaricus bisporus mixed, crush progress decocting,
Filtering, medical fluid merging are concentrated to give concentration filtrate；
(3) above-mentioned concentration filtrate is mixed with the dregs of a decoction, stirs evenly and accesses microorganism fungus kind or lactic acid bacteria, at anaerobic fermentation
Reason, obtains mixed fermentation object；
(4) above-mentioned mixed fermentation object is filtered, filtrate concentrates and purifies through low-temperature reduced-pressure, obtains concentrated broth；
(5) selenium powder, zinc powder are added in above-mentioned concentrated broth, after strong stirring 30min, filling sterilizing is to get arriving
Chinese medicine mixture.
Preferably, microorganism fungus kind described in above-mentioned preparation method is distiller's yeast.
Preferably, when accessing distiller's yeast in above-mentioned preparation method, 0.5% mass ratio of ratio is accessed, adjusts pH to 7-9, temperature
It is 16-31 DEG C, anaerobic fermentation is handled 7-10 days.
Preferably, when accessing lactic acid bacteria in above-mentioned preparation method, PH to 7-9 is adjusted, temperature is 25-37 DEG C, fermentation process
2-5 days.
Preferably, in step (2), it is 1.06-1.12 that medical fluid, which is concentrated under reduced pressure into relative density,；In step (4), filtrate is through low
Purifying is concentrated under reduced pressure in temperature, until relative density is 1.15-1.22, obtains concentrated broth.
Preferably, in step (2), 8 times of amount water are added, are decocted 1 hour after impregnating 2 hours；It is repeated 2 times extraction.
The present invention also provides the preparation methods of the herb mixture described in one kind, comprising the following steps:
(2) chromium powder, agaricus bisporus are prepared into chromium-rich agaricus bisporus；
(2) ginseng, thallus laminariae, semen coicis are pressed into above-mentioned formula rate co-grinding, is extracted with alcohol reflux, repeat to extract one
Secondary, filtration alcohol extract is spare, and the dregs of a decoction save respectively；
(3) dregs of a decoction after fructus lycii, cape jasmine, mulberry leaf, chromium-rich agaricus bisporus and alcohol extracting are mixed and carries out decocting, medical filtration；
(4) protease (0.1%), pectase (0.1%) are accessed after alcohol extract, water being proposed medical fluid mixing concentration, by upper
It states formula rate and adds selenium zinc powder suspension respectively, strong stirring 1.5 hours, in 25-28 DEG C of temperature, pH6.5-7, stirring condition
Under carry out enzymatic hydrolysis 3-5 hours, enzyme deactivation；
(5) medical fluid is concentrated, adjusts pH value 7-10, carries out chelatropic reaction under conditions of 20-42 DEG C of temperature, strong stirring；
(6) cooling, Purification by filtration, filling sterilizing, then pharmaceutical dosage form or food formulation are made according to a conventional method, obtain mixture.
Preferably, the preparation method of the chromium-rich double born of the same parents mushrooms of step (1) is selected from one of following:
A. chromium powder is added in agaricus bisporus compost, the agaricus bisporus of chromium is rich in by normal production management, it is rich
Chromium agaricus bisporus；
B. fresh agaricus bisporus is cut into the small bulk that diameter is 0.2-0.5cm, chromium powder is added to identical as fresh agaricus bisporus
In the pure water of weight, uniform suspension is made in strong stirring 10min, and above-mentioned suspension is then added to fresh agaricus bisporus block
In, after mixing evenly 12-28 DEG C of environment temperature, logical oxygen, without miscellaneous bacteria under the conditions of cultivate 3-7 days, chromium-rich agaricus bisporus is made.
The antitumor principle of the present invention is by following mechanism: (1) being combined into hdac inhibitor target by cyclic peptide, short chain fatty acids
To effect, direct killing tumour cell；(2) blood pressure and blood lipoid of tumor patient is reduced；(3) immunity is promoted, tumour cell is inhibited
Existence.
Microelement is the important substance of human life vigor.Although the microelements such as selenium, chromium, zinc are non-in people's in-vivo content
Chang Wei little, but it all has important physiological action." selenium " is human body and the essential one kind of animal, and keeps body lasting
Health is related, is a kind of very strong antioxidant, and toxic peroxide can be reduced to harmless substance, can peroxynitrite decomposition
Hydrogen is woven with protective effect to cell and group, is allowed to the damage from peroxide, to cell membrane and organelle (mitochondria, particle
Body, lysosome) film have special protective effect.
The active constituent of " zinc " as superoxide dismutase is removed free radical to body antioxidant system and is played to pass weight
The effect wanted.Zinc can induce the synthesis of metallothionein, and metallothionein is strongest one kind in radicals scavenging object again.
" chromium " participates in being formed in human body cell the tolerance factor of glucose.It is maximum that this factor can promote insulin to play
Biological effect.It is higher that " pressure " makes one blood pressure and blood lipoid, often related with chromium is lacked in food.Blood pressure and blood lipoid can be reduced by mending " chromium ",
Help to eliminate dizzy, sleepy isobaric force phenomenon, relieve stress.How scientists begin to research from 1940's
Chromium is mended to patient, by testing repeatedly at once, it has been found that after chromium is absorbed by the body, cannot be utilized, but also want in vivo
Being combined to be formed after amino acid chromium-glucose tolerance factor with intracorporal amino acid can just be immediately used by the body.The present invention generates
The chelating agents such as active peptides and oxalic acid oleanolic acid citric acid form polypeptide -- chromium (III) chelate with trivalent chromium chelatropic reaction,
And its selenium-containing compound, zinc compound etc., to promote the transhipment and absorption of microelement.
We achieve the purpose that reducing blood sugar and blood fat and inhibit tumour using blood circulation and channel invigorating supplementing qi and nourishing yin as therapy.In formula
Thallus laminariae taste is salty, cold in nature, and it is all monarch drug in a prescription with ginseng, coordinated plays that function, which is anti-inflammatory diuresis, hypoglycemic, reducing blood lipid, antitumor
Supplementing qi and nourishing yin, nourishing liver and kidney effect；Ginseng nourishing generate fluid is with tonifying spleen lung, and the prosperous then four dirty gas of lung qi are then prosperous, gas general's blood；Separately
Outside, ginsenoside can enhance body to free radical by improving the activity of the enzymes relevant to free radical resisting such as SOD, CAT
Defence capability.Fructus lycii is nourishing liver and kidney, strengthening the essence blood-nourishing, carrotene, vitamin E, selenium and flavonoids isoreactivity object rich in
Matter；Semen coicis, dual-purpose of drug and food is sweet in flavor, cool in nature, returns spleen, stomach, lung channel, has clearing damp and promoting diuresis, invigorating the spleen to arrest diarrhea, apocenosis eliminating impediment, removing toxic substances to dissipate
The effect of knot enhances QI invigorating tonifying yin function with fructus lycii supplementing qi and nourishing yin, the two taste mutual reinforcements between that promote the production of body fluid to quench thirst, and auxiliary monarch drug in a prescription is nourishing liver and kidney,
Similar phase is asked all as ministerial drug.Cape jasmine, taste bitter and cold, purging intense heat relieving restlessness, clearing heat and promoting diuresis, removing pattogenic heat from the blood and toxic material from the body, externally used detumescence analgesic, promoting blood circulation are logical
Arteries and veins；Mulberry leaf, it is sweet-bitter flavor, cold in nature, it clears liver and improve vision, protein can be promoted to synthesize human body, have the benefits of cooling and activating blood, Stasis through,
Cape jasmine and two taste of mulberry leaf and monarch-minister drug are compatible, and promoting blood circulation makes to mend without stagnant without blood trouble, and has nourishes blood and promotes the production of body fluid, defaecation of promoting blood circulation
Effect, it is all adjutant that two taste mutual reinforcement between compatibilities, which assist monarch-minister drug,.Agaricus bisporus, edible fungus is sweet in flavor, cool in nature, enters intestines, stomach, lung three
Through, there is appetizing, qi-regulating, resolving sputum, happy mind, removing toxic substances, promoting eruption, antiemetic, antidiarrheal, help digestion, refreshing, Pinggan Yang and other effects clearly, " herbal guiding principle
Mesh " in record, mushroom " beneficial stomach, resolving sputum, qi-regulating ", it is used in combination with tonic for we, on the one hand can harmonizing the spleen and stomach,
Promote the efficacy of a drug to absorb, improves nourishing efficiency, on the other hand can prevent cape jasmine thallus laminariae is cold and cool to injure one's stomach again, cause deficiency-cold in spleen and stomach, transport
Change unsmooth, agaricus bisporus and trace elements of selenium, chromium, zinc compatibility, organically combine, make integral formula collaboration efficiently, mend and do not hinder stomach, mend and
Not stagnant, invigorating the spleen defaecation, drink-service safety, coordinating the drug actions of a prescription is to make medicine altogether.
In the present invention, by Chinese medicine ginseng thallus laminariae fructus lycii semen coicis cape jasmine mulberry leaf with the edible mushroom of medical value and micro-
Secondary element match, using the unique diet culinary art in China and modern Chinese herbal medicine extracting method be made have a fixation,
Perfume, taste, shape ticbit.It is that the Chinese medicine of Chinese tradition is gained knowledge the product combined with cooking experience.It " residence doctor in
Food " not only using drug as food, but also food is assigned to medicinal, and medicine borrows food power, eats and helps medicine prestige, the two complements each other, and mutually benefits
It is evident；Not only nutritive value with higher, but also the anti-curing oncoma of hypoglycemic lipid-loweringing.
This prescription increase on the basis of thallus laminariae semen coicis ginseng, fructus lycii will help healthy tendency, improve viscera function,
Enhance the immune function and mobility of human body；Increase mulberry leaf cape jasmine, will activating blood, resolving stasis, dispelling its stasis of blood it is stagnant；Increase edible mushroom mushroom
Mushroom, selenium, chromium, zinc reach comprehensive improve of hypoglycemic antitumor curative effect and effect unique that toxic side effect is not superimposed, therapeutic index
Improve 30% or more.
The present invention be according to traditional Chinese medicine and pharmacy to pathogenetic understanding of " tumour " and its concurrent hyperglycemia hyperlipidemia with
And principle of reatment, with reference to modern microelement " selenium ", chromium, zinc, " cyclic peptide ", " fatty acid " and " hdac inhibitor " pharmacological research
Achievement, from the Ministry of Public Health of China authorizes be food be again drug Chinese medicine in filter out supplementing qi and nourishing yin, blood circulation and channel invigorating, anti-swell
Tumor, and the medicinal material of blood microcirculation and hypoglycemic can be improved, by ginseng, fructus lycii, semen coicis, thallus laminariae, mulberry leaf, cape jasmine and tool
Raw material are formed by the edible fungus mushroom of pharmacological action and microelement etc., are sent out with modern biotechnology enzyme process or microorganism
Ferment method extracts: the pure plants hdac inhibitor compound such as peptide containing selenium and cyclic peptide, short chain fatty acids, has efficiently collaboration anti-
The effect of tumour and reducing blood sugar and blood fat and side effect are not superimposed.
This mixture is the pure natural combination of subtype-selective albumen deacetylase of the completely new chemical structure with the peptide containing selenium
(HDAC) inhibitor belongs to the tumour epigenetic regulation novel drugs of new antitumoral mechanism of action, with traditional oncotherapy
Drug is different, it carries out saturation attack using the epigenetic of cancer cell exception as target spot, and is exempted from by induction patient itself
Epidemic disease monitoring and hypoglycemic lipid-lowering effect and play overall coordination prevention and treatment function of tumor, the pure natural no chemical contamination of food-grade.
Specific embodiment
Below with reference to embodiment, the content of the present invention will be explained in more detail.It should be appreciated that implementation of the invention is not limited to
In the following examples, the accommodation in any form and/or change made to the present invention fall within the scope of the present invention.
In the present invention, if not refering in particular to, all parts by weight, parts by volume relative unit be kilogram, rise, all equipment
It is commercially available with raw material etc. or the industry is common.Method in following embodiments is unless otherwise instructed this
The conventional method in field.
Embodiment 1
Article of the present invention is made of the raw material of following weight parts:
Preparation method:
(1) fresh agaricus bisporus is cut into the small bulk that diameter is 0.2-0.5cm, chromium picolinate is added to and fresh double spores
In the pure water of mushroom identical weight, uniform suspension is made in strong stirring 10min, is then added to above-mentioned suspension fresh double
In spore mushroom block, after mixing evenly 22-28 DEG C of environment temperature, logical oxygen, without miscellaneous bacteria under the conditions of cultivate 7 days, chromium-rich agaricus bisporus is made；
(2) it is added after thallus laminariae, semen coicis, fructus lycii, mulberry leaf, ginseng, cape jasmine and above-mentioned chromium-rich agaricus bisporus being mixed, crushed
8 times of amount water decoct 1 hour after impregnating 2 hours, and medical fluid filtration, filtrate is spare, repeat to extract 1 time by the dregs of a decoction under the same conditions,
Filtering, merging filtrate, being concentrated under reduced pressure into relative density is 1.06-1.12, obtains concentration filtrate；
(3) above-mentioned concentration filtrate is mixed with the dregs of a decoction, accesses distiller's yeast according to 0.5% mass ratio after mixing evenly, adjust PH
To 7-9, temperature is 25-28 DEG C, and anaerobic fermentation is handled 10 days, obtains mixed fermentation object；
(4) it by above-mentioned mixed fermentation object, is filtered after being cooled to room temperature, it is dense to carry out low-temperature reduced-pressure to it after filtrate is purified
Contracting obtains concentrated broth until relative density is 1.15-1.22；
(5) sodium selenite, zinc lactate are added in above-mentioned concentrated broth, it is filling after strong stirring 30min, 100
1 hour is sterilized under the conditions of DEG C to get to Chinese medicine mixture.
Comparative example 1
Raw material is weighed by following recipe ratios
(1) ginseng, thallus laminariae, fructus lycii, mulberry leaf, semen coicis, cassia seed, semen ziziphi spinosae are pressed into above-mentioned formula rate mixed powder
It is broken, with 75% ethyl alcohol (5 times of amounts) refluxing extraction 1.2 hours, repeat to extract once, filtration alcohol extract is spare, and the dregs of a decoction save respectively；
(2) dregs of a decoction carry out decocting 2 times (8 times of water), handle within 1.5 hours every time, medical filtration；(3) alcohol extract, water are proposed medical fluid mixing
High yield peptide bacterial strain is accessed afterwards, i.e. access glutathione bacterial strain, for compressed air (for fostering the spirit of nobility), in 25-28 DEG C of temperature, pH6.5-7
Under the conditions of carry out liquid deep layer fermenting culture 48 hours, centrifugal filtration purifying (remove impurity) heats enzyme deactivation, medical fluid is concentrated,
It is cooling；(4) pH value 6-7 is adjusted, adds chromium powder suspension by above-mentioned formula rate, strong stirring 2 hours, then add by above-mentioned formula rate
Selenium powder suspension, strong stirring 2 hours, filling bundles sealed in time, 105 DEG C of flowing steam sterilizations 30 minutes to get.
Comparative example 2
It is weighted according to the following proportion raw material
Preparation method is: (1) by fructus lycii, pueraria lobata, rhizoma polygonati by above-mentioned formula rate co-grinding, with (5 times of 75% ethyl alcohol
Amount) refluxing extraction 2 hours, it repeats to extract once, filtration alcohol extract is spare, and the dregs of a decoction save respectively；(2) by ginseng, the fruit of glossy privet, acid
Dregs of a decoction mixing after jujube kernel, semen coicis, cassia seed and alcohol extracting carries out decocting 2 times (7 times of water), handles within 1.5 hours every time, medical fluid
Filtering；(3) alcohol extract, water are mentioned after medical fluid mixing is concentrated to 1:4 and accesses acid lipase enzyme (0.2%), acid protease
(0.1%), acidic cellulase (0.1%), acid pectase (0.1%), in 32-50 DEG C of temperature, pH4-5, stirring (80r/
Min fermented and cultured 48 hours under the conditions of), heat enzyme deactivation, and centrifugal filtration purifies (16000r/min centrifuge is after twice), takes supernatant
Medical fluid is concentrated liquid, cooling；(4) pH value 4-6 is adjusted, adds chromium picolinate suspension, strong stirring by above-mentioned formula rate
(1300r/min) 1.5 hours, filling bundles seals in time, 105 DEG C of flowing steam sterilizations 30 minutes to get.
In order to confirm the present invention to antitumor and hypoglycemic lipid-lowering effect effect, we have done following tests respectively.
The antitumor pharmacodynamics test of test example 1
(1), medicament sources and preparation: mixture of the present invention, 20 milliliters of oral solutions are extracted by crude drug 1:1.With preceding with distilled water
It is configured to test the suspension of required concentration.
(2), experimental animal: test uses weight 18-22g kunming mice, male and female dual-purpose, but every batch of test gender is identical,
It is provided by Chinese Academy of Medical Sciences's animal center.
(3), test method: well-grown mouse transplantable tumor Lewis lung cancer, Murine Hepatoma22 and euphorbia egg decoctum are taken, is pressed
Pharmacodynamic experimental technique requires traditional vaccination mouse, and grouping in 24 hours is administered after inoculation.Test is divided into 7 groups, every group
10 mouse, if control group, cyclophosphamide positive drug group (conventional method), embodiment 1 1.25,2.5, tri- dosage group of 3.75g/kg,
1 3.75g/kg dosage group of comparative example, 2 2.5g/kg dosage group of comparative example.Cyclophosphamide 60mg/kg intraperitoneal injection is primary, implements
Example 1, comparative example 1,2 mixture gastric infusion of comparative example continuous 9 times.24 hours execution animals after drug withdrawal, stripping tumor weighing, calculate tumor suppression
Rate, and carry out statistical procedures.It is tested three times respectively.
(4), test result:
1, to the clinical trial of Mice Bearing Lewis Lung Cancer:
Tumour inhibiting rate of the table 1 to Mice Bearing Lewis Lung Cancer
2, to the clinical trial of rat liver cancer H22:
Tumour inhibiting rate of the table 2 to rat liver cancer H22
3, to the clinical trial of S180 sarcoma:
Tumour inhibiting rate of the table 3 to S180 sarcoma
It can be seen that 1 mixture 1.25 of the embodiment of the present invention, 2.5, tri- dosage group of 3.75g/kg from table 1, table 2,3 result of table
There is obvious tumor-inhibiting action to S180 sarcoma, obvious tumor-inhibiting action is demonstrated by Mice Bearing Lewis Lung Cancer, Murine Hepatoma22, and be in
Dose relationship.Wherein Mice Bearing Lewis Lung Cancer curative effect is become apparent from, 1 mixture large dosage 4.74g/kg group of the embodiment of the present invention is average
Inhibitory rate 63.8%.In contrast, 2 mixture of comparative example 1 and comparative example in the application, has under 2.5,3.75g/kg dosage
Tumor-inhibiting action, but with the function and effect for being markedly less than embodiment 1 under concentration.
The test of 2 reducing blood sugar and blood fat of test example
To show that the present invention to lipid-loweringing, hypoglycemic effect effect, through 100 system clinical observations, and selects medical history, disease
Feelings, age, gender and the comparable diabetes of former treatment method and hyperlipemic patients 50 are control group.From be hospitalized and endocrine it is special
Section's outpatient service selects case, and by the diabetes diagnostic criterion that WHO1980 is formulated, the maturity-onset diabetes patient made a definite diagnosis controls in diet
On the basis of therapy and drug (oral hypoglycemic agents and insulin) treatment, fasting blood-glucose 7.2mmol/L person is observation object, wherein
Male 48, female 52 years, the age 27-50 years old, course of disease half a year was to 20 years.Test group deactivates lipid-lowering medicine, clothes on the basis of original treatment
1 composition medicine of the embodiment of the present invention.Control group, routinely hypoglycemic, lipid-lowering therapy, wherein hypoglycemic medicine, kind administration dosage with
Test group matches, and lipid-lowering medicine uses import fenofibrate.Fasting blood-glucose and blood lipid, observation period two are inspected periodically using same procedure
A month, 30 days as one therapeutic course.
Instructions of taking: 1 mixture 20ml/ of the embodiment of the present invention is primary, takes daily 3 times, boiled water is taken after mixing it with water before meals.Clinical test results
Such as table 4, table 5.
Table 4 takes insulin before and after 1 mixture of the embodiment of the present invention, Diamicron with being in and change of blood sugar
Table 5 takes lipid-loweringing survival dose and the variation of blood lipid mean concentration before and after 1 mixture of the embodiment of the present invention
From table 4, it can be seen that fasting plasma glucose concentration and hypoglycemic medicine dosage are substantially similar before two groups of tests, and P < 0.05, examination
It tests blood glucose after group takes 1 mixture of the embodiment of the present invention to decline, hypoglycemic survival dose but reduces 1/3-1/2 than originally.
Table 5 statistics indicate that, serum cholesterol and triglycerides mean concentration are without significant difference before two groups of tests；But test group
After 1 mixture of the embodiment of the present invention, any other lipid-lowering medicine is not used, that is, can reach lipid-loweringing purpose.
Test statistics show: therapeutic test is made to 100 diabetes and hyperlipemic patients with 1 mixture of the embodiment of the present invention,
Obtain significant curative effect accounts for 53%.Substantially 42% is effectively accounted for, accounts for 5% in vain, total effective rate is up to 95%.
Although inventor has done more detailed elaboration to technical solution of the present invention and has enumerated, it should be understood that right
For the those skilled in the art of this field one, modifications to the embodiments described above may be made or uses equivalent alternative solution, this
It is it is clear that these made without departing from theon the basis of the spirit of the present invention are modified or changed to those skilled in the art
Into falling within the scope of the claimed invention.
Claims (10)
1. a kind of herb mixture of the anti-curing oncoma of hypoglycemic, made of the raw material of following weight proportion:
It is 3-15 parts of ginseng, 5-20 parts of thallus laminariae, 5-25 parts of fructus lycii, 4-16 parts of mulberry leaf, 10-30 parts of semen coicis, 4-20 parts of cape jasmine, double
30-160 parts of spore mushroom, 0.00001-0.002 parts of selenium, 0.00005-0.002 parts of zinc, 0.00002-0.001 parts of chromium.
2. herb mixture according to claim 1, made of the raw material of following weight proportion: 3-8 parts of ginseng, thallus laminariae 5-12
Part, 8-15 parts of fructus lycii, 5-10 parts of mulberry leaf, 15-20 parts of semen coicis, 4-10 parts of cape jasmine, 100-160 parts of agaricus bisporus, selenium 0.0001-
0.001 part, 0.0001-0.001 parts of zinc, 0.0001-0.001 parts of chromium.
3. any one of -2 herb mixture according to claim 1, made of the raw material of following weight proportion: 3 parts of ginseng, elder brother
9 parts of cloth, 10 parts of fructus lycii, 8 parts of mulberry leaf, 15 parts of semen coicis, 8 parts of cape jasmine, 150 parts of agaricus bisporus, 0.0006 part of selenium, zinc 0.0009
Part, 0.0004 part of chromium.
4. a kind of preparation method of the described in any item herb mixtures of claim 1-3, comprising the following steps:
(1) chromium powder, agaricus bisporus are prepared into chromium-rich agaricus bisporus；
(2) thallus laminariae, semen coicis, fructus lycii, mulberry leaf, ginseng, cape jasmine and chromium-rich agaricus bisporus mixed, crush progress decocting, filtered,
Medical fluid merging is concentrated to give concentration filtrate；
(3) above-mentioned concentration filtrate being mixed with the dregs of a decoction, stirs evenly access microorganism fungus kind or lactic acid bacteria, anaerobic fermentation is handled,
Obtain mixed fermentation object；
(4) above-mentioned mixed fermentation object is filtered, filtrate concentrates and purifies through low-temperature reduced-pressure, obtains concentrated broth；
(5) selenium powder, zinc powder are added in above-mentioned concentrated broth, after strong stirring 30min, filling sterilizing is to get into sheet
Medicament mixture.
5. method according to claim 4, the microorganism fungus kind is distiller's yeast.
6. when accessing distiller's yeast, accessing 0.5% mass ratio of ratio according to any one of claim 4-5 the method, adjusting pH to 7-
9, temperature is 16-31 DEG C, and anaerobic fermentation is handled 7-10 days.
7. when accessing lactic acid bacteria, adjusting PH to 7-9, temperature is 25-37 DEG C, hair according to any one of claim 4-6 the method
Ferment is handled 2-5 days.
8. in step (2), it is 1.06- that medical fluid, which is concentrated under reduced pressure into relative density, according to any one of claim 4-7 the method
1.12；In step (4), filtrate concentrates and purifies through low-temperature reduced-pressure, until relative density is 1.15-1.22, obtains concentrated broth；
In step (2), 8 times of amount water are added, are decocted 1 hour after impregnating 2 hours；It is repeated 2 times extraction.
9. a kind of preparation method of the described in any item herb mixtures of claim 1-3, comprising the following steps:
(1) chromium powder, agaricus bisporus are prepared into chromium-rich agaricus bisporus；
(2) ginseng, thallus laminariae, semen coicis are pressed into above-mentioned formula rate co-grinding, are extracted with alcohol reflux, repeat to extract once,
Filtration alcohol extract is spare, and the dregs of a decoction save respectively；
(3) dregs of a decoction after fructus lycii, cape jasmine, mulberry leaf, chromium-rich agaricus bisporus and alcohol extracting are mixed and carries out decocting, medical filtration；
(4) protease (0.1%), pectase (0.1%) are accessed after alcohol extract, water being proposed medical fluid mixing concentration, is matched by above-mentioned
Square ratio adds selenium zinc powder suspension respectively, strong stirring 1.5 hours, under 25-28 DEG C of temperature, pH6.5-7, stirring condition into
Row enzymatic hydrolysis 3-5 hours, enzyme deactivation；
(5) medical fluid is concentrated, adjusts pH value 7-10, carries out chelatropic reaction under conditions of 20-42 DEG C of temperature, strong stirring；
(6) cooling, Purification by filtration, filling sterilizing, then pharmaceutical dosage form or food formulation are made according to a conventional method, obtain mixture.
10. the preparation method of the chromium-rich double born of the same parents mushrooms of step (1) is in as follows according to any one of claim 4-9 the method
It is a kind of:
A. chromium powder is added in agaricus bisporus compost, the agaricus bisporus of chromium is rich in by normal production management, it is chromium-rich double
Spore mushroom；
B. fresh agaricus bisporus is cut into the small bulk that diameter is 0.2-0.5cm, chromium powder is added to and fresh agaricus bisporus identical weight
Pure water in, uniform suspension is made in strong stirring 10min, then above-mentioned suspension is added in fresh agaricus bisporus block,
After mixing evenly 12-28 DEG C of environment temperature, logical oxygen, without miscellaneous bacteria under the conditions of cultivate 3-7 days, chromium-rich agaricus bisporus is made.
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| CN114209055A (en) * | 2021-12-20 | 2022-03-22 | 星工坊(苏州)生物科技有限公司 | Composition helpful for blocking oil absorption, preparation method and preparation |
| CN115245200A (en) * | 2021-04-02 | 2022-10-28 | 孙建国 | Health food for preventing and treating hypertension, hyperlipidemia and hyperglycemia |
| CN116676241A (en) * | 2023-08-02 | 2023-09-01 | 四川好培养生物工程有限公司 | Chinese medicinal composition extract and bacterial culture medium |
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| CN108853389A (en) * | 2018-08-15 | 2018-11-23 | 杨京润 | A kind of anti-oxidant antitumor herb fermenting agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108853389A (en) * | 2018-08-15 | 2018-11-23 | 杨京润 | A kind of anti-oxidant antitumor herb fermenting agent and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115245200A (en) * | 2021-04-02 | 2022-10-28 | 孙建国 | Health food for preventing and treating hypertension, hyperlipidemia and hyperglycemia |
| CN114209055A (en) * | 2021-12-20 | 2022-03-22 | 星工坊(苏州)生物科技有限公司 | Composition helpful for blocking oil absorption, preparation method and preparation |
| CN116676241A (en) * | 2023-08-02 | 2023-09-01 | 四川好培养生物工程有限公司 | Chinese medicinal composition extract and bacterial culture medium |
| CN116676241B (en) * | 2023-08-02 | 2023-10-27 | 四川好培养生物工程有限公司 | Chinese medicinal composition extract and bacterial culture medium |
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