CN102294022B - SOD (superoxide dismutase) preparation and preparation method thereof - Google Patents
SOD (superoxide dismutase) preparation and preparation method thereof Download PDFInfo
- Publication number
- CN102294022B CN102294022B CN2010102107005A CN201010210700A CN102294022B CN 102294022 B CN102294022 B CN 102294022B CN 2010102107005 A CN2010102107005 A CN 2010102107005A CN 201010210700 A CN201010210700 A CN 201010210700A CN 102294022 B CN102294022 B CN 102294022B
- Authority
- CN
- China
- Prior art keywords
- sod
- liquid
- mother solution
- controlled
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides an SOD (superoxide dismutase) preparation and a preparation, stabilization and maintenance technology thereof. The technique for preparing the novel proteinase activity substance comprises the following steps: by using stauntonvine, actinidia and Rosa Roxburghii Tratt as the main raw material, fermenting, carrying out wall breaking to release SOD, extracting by cold soaking, carrying out rough filtration, carrying out heat treatment on the crude enzyme liquid, carrying out negative-pressure centrifugal separation, concentrating by CO2 supercritical extraction, carrying out molecular modification and protection by using a modification liquid, and stabilizing the enzyme activity. The preparation comprises a liquid form and a freeze-dried powder form.
Description
Technical field
It is a kind of take Caulis et folium stauntoniae, Fructus actinidiae chinensis, Fructus Rosae Normalis as primary raw material that the art of this patent provides, and adopts fermentation, merceration extraction, centrifugalize, breaking cellular wall to discharge SOD, the molecular sieve screening is concentrated, decorating liquid carries out molecular modification protection stabilized enzyme vigor Novel protease active substance and produce, stablize and maintenance technology and the SOD preparation produced with this technology.
Background technology
SOD (Superoxide dismutase) is described as by World Health Organization (WHO) the medicinal enzyme that 21 century has the development and application prospect most.
In decades; unremitting effort through various countries' scientists is studied, tests, is tested; consistent proof; in a single day external source SOD enters in human body; enter blood; enter microcirculation, just can effectively protect the living matters such as lipid in body cell, protein, DNA, cell membrane, make them avoid unnecessary ultra-oxygen anion free radical O in human body
2-Destruction with hydroxy radical OH, the immunologic function of enhancing body cell and various vitals, to the treatment autoimmune disease, viral disease, cardiovascular disease, as various inflammation, hypertension, thrombotic disease, diabetes, rheumatic arthritis, lupus erythematosus and cancer etc., special efficacy is arranged, radiation is had to protective action.So, SOD be known as 21 century have most the exploitation future medicinal enzyme.
Yet the existing SOD technology of producing has multiplely, comprises from plant and fruit, producing SOD, with microbial fermentation processes, produces SOD, and prepares Rh-SOD with the method for gene recombinaton.In plant, that more existence is Cu-Zn-SOD, and Mn-SOD is less.The invention provides a kind of method for preparing composite S OD preparation, this mother solution comprises Cu-Zn-SOD, and Mn-SOD, makes in addition the yield of SOD be greatly improved.
Summary of the invention
The application's goal of the invention, be to provide a kind of composite S OD preparation and preparation method thereof.
A kind of SOD preparation preparation method, its step comprise batching successively, clean drain, broken, add hot fermentation, breaking cellular wall discharges SOD, merceration extraction, coarse filtration, heat treated crude enzyme liquid, negative pressure centrifugalize, CO
2Supercritical extraction, add decorating liquid to carry out molecular modification protection stabilized enzyme vigor and by SOD mother solution sealing anoxybiotic lucifuge packing/or utilize freeze drying technology that SOD mother solution finished product is made to lyophilized powder sealing lucifuge packing.
Wherein concrete steps are:
(1) batching.
Require raw fruit, free from admixture, without going mouldy, without decayed fruit;
Raw material comprises: be no less than 60% Caulis et folium stauntoniae, Fructus Rosae Normalis is no less than 25%
(2) clean and drain: raw material is cleaned and drains.
(3) fragmentation: it is the 30-50 order that the raw material after draining is crushed to fineness degree.
(4) add hot fermentation
Controlling the heating fermentation temperature is 30-35 ℃, and pH value is adjusted to 7, and yeast phase is three to five days.Sweat is with stirring, and stir speed (S.S.) is 100-150 rev/min.
Fermentation can be used different strains, for example, and escherichia coli, yeast, brewer yeast bacterium, bacillus cereus etc.Wherein preferred strain is saccharomyces cerevisiae (Saccharomyces cerevisiae CICC1308).
(5) breaking cellular wall discharges SOD
Adopt the supersonic wave wall breaking technology to a large amount of brewer yeast bacterium breaking cellular walls that generate of sweat, this breaking cellular wall comprises brokenly cell wall and the intracellular mitochondrial wall of microorganism, makes it to discharge inherent SOD.Ultrasonic power is 100-200W; Ultrasonic time is 5-10 minute.Supersound process simultaneously can also be further broken and intracellular mitochondrion wall is further broken by the fresh fruit cell wall, and this will be in cytoplasm, with Intramitochondrial SOD, discharging.Thereby increase SOD and yield, namely increase quantum of output.
(6) merceration extraction.
Take alcoholic strength as the pure sticky rice wine of 30 ° as leaching liquid, temperature is controlled between subzero 13 ℃ of subzero 11-, and pH value is controlled to be 7,72 hours time.Under sub-zero temperature, not yet the fresh fruit cell volumetric expansion of breaking cellular wall is broken, and discharges SOD.
(7) coarse filtration.
Temperature is controlled at below 20 ℃, with 3000-4000 rev/min of centrifugalize, obtains coarse filtration liquid, and enzyme activity is controlled between 800-1000u/ml, and pH value is adjusted to 7-7.2.Remaining coarse filtration waste can be made raw material or the feedstuff of wine afterwards.
If need further to extract, also the coarse filtration waste can be performed step again to the extraction of (six) merceration, then remake raw material or the feedstuff of wine.
(8) heat treated crude enzyme liquid.
Temperature is controlled at 60-70 ℃, and the time is controlled at 5-10 minute, and the heat treated crude enzyme liquid, to remove foreigh protein removing.This be because SOD as metalloenzyme, Heat stability is good, and other foreign protein poor heat resistance, so can pass through thermal change, make it to precipitate.
(9) negative pressure centrifugalize.
At room temperature progressively adding mass percent is that the common salt aqueous solution of 24%-27% enters in the crude enzyme liquid body, pH value is transferred to 7.4-7.8, stir homogenate 90 minutes, at this moment foreign protein gradually flocculation sediment get off, under the environment of negative pressure, with 6000 rev/mins-8000 rev/mins centrifugal 20 minutes, enzyme activity still is controlled between 800-1000u/ml; Centrifugal waste can be made the oral liquid raw material.
(10) CO
2Supercritical extraction and concentrated.
Adopt CO
2Supercritical extraction with alcohol recovery device, pass through CO
2Supercritical extraction can be removed salt solution, and by alcohol recovery device, recovered alcohol, obtain being concentrated to 10% the thick finished product of SOD mother solution of original volume, by concentrated, SOD mother solution enzyme activity is increased between 8000-10000u/ml.
(11) molecular modification.
With the tea polyphenols that pure water is 95% by PEG 8000 (PEG8000) and purity, be made into composite modified liquid, the composite quality ratio is 75: 25; Decorating liquid homogenate adds in the thick finished product of SOD mother solution, and adding mass ratio is 1: 10, stirs at room temperature the 90min typing, obtains SOD mother solution finished product, and enzyme activity is controlled between 7000-9000u/ml.The molecular modification purpose is that tea polyphenols can prevent that SOD is oxidized.
(12) SOD finished product.
The SOD finished product is divided into two kinds of forms, and it 1 is the SOD mother solution that above-mentioned molecular modification is crossed, and with liquid condition, exists; It 2 is by the freeze-dry process technology, and the SOD mother solution that above-mentioned molecular modification is crossed is made to lyophilized powder.As lyophilized powder, be suitable for preserving, easily keep active.As SOD mother solution finished product, put in storage, require packaging material must adopt the lucifuge material, the SOD mother solution can fill in lighttight vial or plastic bottle, SOD lyophilized powder is packaged in the lucifuge packaging bag of sealing, during packing, all need under starvation and the condition in lucifuge, carry out.
The application is take multiple wild SHANGUO as raw material, by fermentation technology, cultivate and produce the superoxide dismutase SOD in plant rennet, make the stable maintenance of its active longer-term, and be effectively applied in various biologics, food, skin care item, animal feed and crops growing agent.
the process route that the application asks for protection, its advanced and originality are that it has abandoned the process route that chemical agent that domestic and international extraction SOD continues to use always separates column chromatography, have initiated composite fermentation, merceration extraction, centrifugalize, CO
2supercritical extraction, decorating liquid carries out the process routes such as molecular modification protection stabilized enzyme vigor, its advance and originality have been to abandon the large chemical agent (hydrochloric acid of toxic and side effects, ether, acetone etc.) make eluting and separating medium, abandoned loaded down with trivial details upper column chromatography concentration method, avoided product to become the toxic and side effects that finished product and workshop is caused by chemical agent, water and low alcoholic degree sticky rice wine are made eluting and extract, with high speed centrifuge, making physical property separates, the gained finished product is natural pure, side effect without chemical agent infects, be convenient to again industrialized great production, whole technical process has improved the interest rate of SOD, improved efficiency, reduced cost.For all extract the process route of SOD without comparability both at home and abroad.
The extracting method that the application asks for protection screens fermentation, extracts, separates, is concentrated to the whole Technologies such as molecular modification from the raw material prescription; raw material is carried out to the multilamellar utilization; to separating concentrated waste and abandoning liquid; all recoverable, none abandons, and is a project environmental type, economizing type and series of products.
The SOD enzyme activity that extracts due to the application's method is far above existing SOD extract, therefore the SOD of this method extraction is to eliminating the damage of negative oxygen anion free radical to body cell, antioxidation, radioprotective, antiviral, anticancer change, improve looks U.S. face, slow down aging etc. have special-effect, the enhancing greatly of effect has also enlarged its effective scope of application, make it to be widely used in food, medicine, cosmetics, have unique practical value.
Simultaneously, the present invention creatively merges two kinds of different SOD extraction processes of microorganism fermentation, cell wall breaking and the direct cell wall breaking of plant fruit by test of many times, not only improved the output of SOD, and enriched the kind of the SOD that extracts, both contain Cu-Zn-SOD, contained again Mn-SOD.And Mn-SOD is human body viscera, as liver and heart, and the antioxidation dismutase that needs most
The accompanying drawing explanation
Fig. 1 is the application's process chart.
The specific embodiment
Referring to Fig. 1, the application's SOD mother solution extraction step is as follows:
Its concrete operations are as follows:
(1) batching
Because Caulis et folium stauntoniae, Fructus actinidiae chinensis and Fructus Rosae Normalis are referred to as the king in fruit, contain very abundant SOD, multivitamin, biological ketone, various trace elements and various active composition.So we select them as raw material.Its objective is the effective active components that assembly is abundanter.Certainly, also can, with fruit and the plant of single kind, also can, with fruit and the plant of more kind, can produce the SOD preparation.
Raw material comprises: Caulis et folium stauntoniae, Fructus actinidiae chinensis, Fructus Rosae Normalis.Its ratio is take Caulis et folium stauntoniae as Main Ingredients and Appearance, and Caulis et folium stauntoniae is no less than 60%, and Fructus Rosae Normalis is no less than 25%.
(2) clean and drain
Raw material is cleaned and drains.
(3) fragmentation
It is the 30-50 order that raw material after draining is crushed to fineness degree.
(4) add hot fermentation
Controlling the heating fermentation temperature is 30-35 ℃, and pH value is adjusted to 7, and yeast phase is three to five days.Sweat is with stirring, and stir speed (S.S.) is 100-150 rev/min.
Can use different strains, for example, escherichia coli, yeast, brewer yeast bacterium, bacillus cereus etc.We select saccharomyces cerevisiae (Saccharomyces cerevisiae CICC1308) at preferred forms.In mentioned microorganism, often there is Mn-SOD.Under the anaerobic condition of fermentation, yeast cells is in order to remain normal metabolism, can form the cytoprotective material that comprises superoxide dismutase SOD.Stimulate to external world and adverse circumstance environment generation stress, improve its adaptive faculty.Environmental threat stimulates and increases, and just produces more SOD.The multiple components such as sugar in sweat utilization fruit oar, produce ethanol simultaneously.
(5) breaking cellular wall discharges SOD
Adopt the supersonic wave wall breaking technology to a large amount of brewer yeast bacterium breaking cellular walls that generate of sweat, this breaking cellular wall comprises brokenly cell wall and the intracellular mitochondrial wall of microorganism, makes it to discharge inherent SOD.Ultrasonic power is 100-200W; Ultrasonic time is 5-10 minute.Supersound process simultaneously can also be further broken and intracellular mitochondrion wall is further broken by the fresh fruit cell wall, and this will be in cytoplasm, with Intramitochondrial SOD, discharging.Thereby increase SOD and yield, namely increase quantum of output.
(6) merceration extraction
Take alcoholic strength as the pure sticky rice wine of 30 ° as leaching liquid, temperature is controlled between subzero 11-13 ℃, and pH value is controlled at 7,72 hours time.Under sub-zero temperature, not yet the fresh fruit cell volumetric expansion of breaking cellular wall is broken, and discharges SOD.
(7) coarse filtration
Temperature is controlled at below 20 ℃, with 3000-4000 rev/min of centrifugalize, the persistent period be 15-20 minute coarse filtration liquid, enzyme activity is controlled between 800-1000u/ml, pH transfers to 7-7.2.Remaining coarse filtration waste can be made raw material or the feedstuff of wine afterwards.
If need further to extract, also the coarse filtration waste can be performed step again to the extraction of (six) merceration, then remake raw material or the feedstuff of wine.
(8) heat treated crude enzyme liquid
Temperature is controlled at 60-70 ℃, and the time is controlled at 5-10 minute, and the heat treated crude enzyme liquid, to remove foreigh protein removing.This be because SOD as metalloenzyme, Heat stability is good, and other foreign protein poor heat resistance, so can pass through thermal change, make it to precipitate.
(9) negative pressure centrifugalize
At room temperature progressively adding mass percent is that the common salt aqueous solution of 24%-27% enters in the crude enzyme liquid body, pH value is transferred to 7.4-7.8, stir homogenate 90 minutes, at this moment foreign protein gradually flocculation sediment get off, under the environment of negative pressure, with 6000 rev/mins-8000 rev/mins centrifugal 20 minutes, enzyme activity still is controlled between 800-1000u/ml; Centrifugal waste can be made the oral liquid raw material.
(10) CO
2Supercritical extraction and concentrated
Adopt CO
2Supercritical extraction with alcohol recovery device, pass through CO
2Supercritical extraction can be removed Sal, and by alcohol recovery device, recovered alcohol, obtain being concentrated to 10% the thick finished product of SOD mother solution of original volume, and due to concentrated, SOD mother solution enzyme activity will be greatly improved, and be controlled between 8000-10000u/ml.
(11) molecular modification
With the tea polyphenols that pure water is 95% by PEG 8000 (PEG8000) and purity, be made into composite modified liquid, the composite quality ratio is 75: 25; Decorating liquid homogenate enters in the thick finished product of SOD mother solution, and adding mass ratio is 1: 10, stirs at room temperature the 90min typing, obtains SOD mother solution finished product, and enzyme activity is controlled between 7000-9000u/ml.Its purpose is that tea polyphenols can prevent that SOD is oxidized.
(12) SOD finished product
The SOD finished product is divided into two kinds of forms, and it 1 is the SOD mother solution that above-mentioned molecular modification is crossed, and with liquid condition, exists; It 2 is by freeze-dry process equipment, and the SOD mother solution that above-mentioned molecular modification is crossed is made to lyophilized powder.As lyophilized powder, be suitable for preserving, easily keep its activity.As SOD mother solution finished product, put in storage, require packaging material must adopt the lucifuge material, the SOD mother solution can fill in lighttight vial or plastic bottle, SOD lyophilized powder is packaged in the lucifuge packaging bag of sealing, during packing, all need under starvation and the condition in lucifuge, carry out.
The present invention relates to produce the SOD preparation with Caulis et folium stauntoniae, Fructus actinidiae chinensis and Fructus Rosae Normalis; the those of ordinary skill of this professional skill field; fully can be according to instruction of the present invention; increase and reduce the kind of fruit and plant; change the proportioning between fruit and plant; produce the SOD preparation, but such solution is all in protection domain of the present invention.
Claims (2)
1. the preparation method of a SOD preparation, its concrete steps are:
(1) batching;
Require raw fruit, free from admixture, without going mouldy, without decayed fruit;
Raw material comprises: be no less than 60% Caulis et folium stauntoniae, Fructus Rosae Normalis is no less than 25%;
(2) clean and drain: raw material is cleaned and drains;
(3) fragmentation: it is the 30-50 order that the raw material after draining is crushed to fineness degree;
(4) add hot fermentation:
Use the fermentation of brewer yeast bacterium, controlling the heating fermentation temperature is 30-35 ℃, and pH value is adjusted to 7, and yeast phase is three to five days; Sweat is with stirring, and stir speed (S.S.) is 100-150 rev/min;
(5) breaking cellular wall discharges SOD:
Adopt the supersonic wave wall breaking technology to a large amount of zymocyte breaking cellular walls that generate of sweat, make described zymocyte discharge inherent SOD, ultrasonic power is 100-200W; Ultrasonic time is 5-10 minute;
(6) merceration extraction:
Take alcoholic strength as the pure sticky rice wine of 30 ° as leaching liquid, temperature is controlled between subzero 13 ℃ of subzero 11-, and pH value is controlled to be 7,72 hours time; Under sub-zero temperature, not yet the fresh fruit cell volumetric expansion of breaking cellular wall is broken, and discharges SOD;
(7) coarse filtration:
Temperature is controlled at below 20 ℃, with 3000-4000 rev/min of centrifugalize, obtains coarse filtration liquid, and enzyme activity is controlled between 800-1000u/ml, and pH value is adjusted to 7-7.2;
(8) heat treated crude enzyme liquid:
Temperature is controlled at 60-70 ℃, and the time is controlled at 5-10 minute, and the heat treated crude enzyme liquid, to remove foreigh protein removing;
(9) negative pressure centrifugalize:
At room temperature progressively adding mass percent concentration is that the common salt aqueous solution of 24%-27% enters in crude enzyme liquid, pH value is adjusted to 7.2-7.4, stir homogenate 90 minutes, make foreign protein gradually flocculation sediment get off, under the environment of negative pressure, with 6000 rev/mins-8000 rev/mins centrifugal 20 minutes, enzyme activity still is controlled between 800-1000u/ml;
(10) CO
2Supercritical extraction and concentrated:
Adopt CO
2Supercritical extraction with alcohol recovery device, pass through CO
2Supercritical extraction can be removed salt solution, and by alcohol recovery device, recovered alcohol, obtain being concentrated to 10% the thick finished product of SOD mother solution of original volume, by concentrated, SOD mother solution enzyme activity is increased between 8000-10000u/ml;
(11) molecular modification:
With the tea polyphenols that pure water is 95% by PEG 8000 (PEG8000) and purity, be made into composite modified liquid, PEG 8000 and 95% tea polyphenols mass ratio are 75: 25; Decorating liquid homogenate adds in the thick finished product of SOD mother solution, and adding mass ratio is 1: 10, stirs at room temperature the 90min typing, obtains SOD mother solution finished product, and enzyme activity is controlled between 7000-9000u/ml;
(12) SOD finished product:
By described SOD mother solution finished product, with liquid condition, exist fill to put in storage in lighttight vial or plastic bottle; Perhaps by the freeze-dry process technology, SOD mother solution finished product is made to lyophilized powder, SOD lyophilized powder is packaged in the lucifuge packaging bag of sealing; When the fill of above-mentioned SOD mother solution or SOD lyophilized powder packing, all need under starvation and the condition in lucifuge, carry out.
2. the lyophilized powder for preparing of the SOD mother solution for preparing of the preparation method of SOD preparation according to claim 1 or SOD mother solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102107005A CN102294022B (en) | 2010-06-28 | 2010-06-28 | SOD (superoxide dismutase) preparation and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102107005A CN102294022B (en) | 2010-06-28 | 2010-06-28 | SOD (superoxide dismutase) preparation and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102294022A CN102294022A (en) | 2011-12-28 |
| CN102294022B true CN102294022B (en) | 2013-11-20 |
Family
ID=45354854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102107005A Active CN102294022B (en) | 2010-06-28 | 2010-06-28 | SOD (superoxide dismutase) preparation and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102294022B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747054B (en) * | 2012-07-27 | 2015-05-13 | 田瑛 | Process for extracting superoxide dismutase (SOD) |
| CN105838685A (en) * | 2016-04-26 | 2016-08-10 | 句容市东方紫酒业有限公司 | Method for extracting SOD (superoxide dismutase) from mulberry wine lees |
| CN109845594A (en) * | 2016-05-30 | 2019-06-07 | 路平 | A kind of implantation methods of multienzyme crops containing SOD and its application |
| CN106265262A (en) * | 2016-08-25 | 2017-01-04 | 周荣 | A kind of preparation method of natural cosmetic water |
| CN106333904A (en) * | 2016-11-07 | 2017-01-18 | 辽宁石油化工大学 | Method for preparing pure natural care components in cosmetics by natural degradation of fruits |
| CN107022531A (en) * | 2017-03-31 | 2017-08-08 | 夏胜芳 | A kind of method for extracting the smart enzymes of SOD after biofermentation from rich SOD plants (triticale, rice bran oil chaff powder) |
| CN107278906B (en) * | 2017-08-09 | 2018-11-23 | 朱晓瑜 | A kind of Moringa implantation methods rich in SOD |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090062342A (en) * | 2007-12-13 | 2009-06-17 | 덕성여자대학교 산학협력단 | Extracts from elsholtzia splendens having antioxidative activity |
-
2010
- 2010-06-28 CN CN2010102107005A patent/CN102294022B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090062342A (en) * | 2007-12-13 | 2009-06-17 | 덕성여자대학교 산학협력단 | Extracts from elsholtzia splendens having antioxidative activity |
Non-Patent Citations (4)
| Title |
|---|
| 刘宏 等.利用几种生物原料提取SOD的比较研究.《贵州农业科学》.2009,第37卷(第3期),92-93. |
| 利用几种生物原料提取SOD的比较研究;刘宏 等;《贵州农业科学》;20091231;第37卷(第3期);92-93 * |
| 陆俊.微生物发酵对大蒜SOD活性的影响及其分离提取工艺研究.《中国优秀硕士学位论文全文数据库(硕士) 农业科技辑》.2006,19-47. * |
| 韩薇妍.超氧化物歧化酶修饰产物结构和性能研究.《北京化工大学学报（自然科学版）》.2010,第37卷(第2期),117-120. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102294022A (en) | 2011-12-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102294022B (en) | SOD (superoxide dismutase) preparation and preparation method thereof | |
| CN104013657B (en) | A kind of American ginseng medicine extracts after saponin(e microbial fermentation extracting method again | |
| CN103271951B (en) | Method for preparing cordyceps preparations with high adenosine contents | |
| CN108324633A (en) | A kind of preparation method and products thereof of fermentative brown rice stoste used for cosmetic | |
| CN107550961B (en) | A kind of method that microbe Rapid Fermentation prepares ginseng/American ginseng extract | |
| CN102337313B (en) | Method for preparing trehalose | |
| CN107708445A (en) | The manufacture method of bamboo extractive from fermentative and immunity effect agent | |
| CN102120779B (en) | Efficient extraction technology of ganodorma lucidum fermention mycelium homogeneous polysaccharides | |
| ES2526873T3 (en) | Procedure for the preparation of a fermented natural product | |
| CN101450089B (en) | Preparation method of mangrove extract liquid | |
| CN102344862B (en) | Production method of burdock wine | |
| CN106108003A (en) | A kind of preparation method rich in the black ginseng of rare ginsenoside Rh2 | |
| CN102863547A (en) | Method for extracting active polysaccharides from pleurotus eryngii and preparing functional beverage of active polysaccharides | |
| CN111269954B (en) | Method for preparing ganoderma lucidum spore powder exine crude polysaccharide by using enzyme method assisted ultrasound | |
| CN102652556B (en) | Preparation method of instant kudzuvine root particles | |
| CN109820778A (en) | A kind of hickory chick biofermentation bacterium solution composite skin care product and preparation method thereof | |
| CN104403020A (en) | Semi-bionic lentinan extraction method | |
| CN105200102B (en) | Method for extracting glutathione from candida utilis fermentation liquor | |
| CN106539075A (en) | A kind of preparation method of efficient absorption sea cucumber nutrient buccal tablet | |
| CN111808905B (en) | Method for extracting walnut oligopeptide from low-temperature squeezed hickory cake and application thereof | |
| CN107307337A (en) | A kind of chlorella tablet and preparation method thereof | |
| CN111991836A (en) | Method for extracting nutrient from medicinal and edible plant | |
| JPS6024169A (en) | Preparation of extract of pollen | |
| KR20200046527A (en) | Functional mulberry leaf extract and manufacturing method thereof | |
| CN105199008A (en) | Decolorizing method for Huping jujube polysaccharide solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |