CN112504796A - Integrated cell treatment device - Google Patents
Integrated cell treatment device Download PDFInfo
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- CN112504796A CN112504796A CN202011400561.2A CN202011400561A CN112504796A CN 112504796 A CN112504796 A CN 112504796A CN 202011400561 A CN202011400561 A CN 202011400561A CN 112504796 A CN112504796 A CN 112504796A
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- 238000011282 treatment Methods 0.000 title description 6
- 230000007246 mechanism Effects 0.000 claims abstract description 56
- 238000004043 dyeing Methods 0.000 claims abstract description 42
- 239000011521 glass Substances 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 230000017525 heat dissipation Effects 0.000 claims description 4
- 238000009434 installation Methods 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 abstract description 51
- 210000001175 cerebrospinal fluid Anatomy 0.000 abstract description 27
- 238000007689 inspection Methods 0.000 abstract description 8
- 230000002380 cytological effect Effects 0.000 abstract description 3
- 210000003128 head Anatomy 0.000 description 18
- 210000004556 brain Anatomy 0.000 description 6
- 210000000278 spinal cord Anatomy 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 239000012192 staining solution Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 210000003169 central nervous system Anatomy 0.000 description 2
- 210000002987 choroid plexus Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 210000001032 spinal nerve Anatomy 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 206010048612 Hydrothorax Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 208000025222 central nervous system infectious disease Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000003748 differential diagnosis Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000000185 intracerebroventricular administration Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 210000004880 lymph fluid Anatomy 0.000 description 1
- 230000001926 lymphatic effect Effects 0.000 description 1
- 210000002418 meninge Anatomy 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 210000002330 subarachnoid space Anatomy 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the technical field of cytological examination sample processing devices, and particularly relates to an integrated cell processing device which comprises a shell, a centrifugal mechanism, a sheet making mechanism and a dyeing mechanism, wherein the centrifugal mechanism, the sheet making mechanism and the dyeing mechanism are arranged in the shell, the shell comprises an upper shell, a lower shell and a top cover, the centrifugal mechanism comprises a driving motor and a centrifugal cylinder, a centrifugal tube is arranged in the centrifugal cylinder, the centrifugal tube is detachably arranged in the centrifugal cylinder, an accommodating cavity is arranged in the upper shell, a centrifugal groove and a motor groove are formed in the bottom of the accommodating cavity, a gear is fixedly connected to an output shaft of the driving motor, a first toothed ring is integrally formed on the outer wall of the centrifugal cylinder, the sheet making mechanism comprises a sheet placing frame and a plurality of glass slides, the dyeing mechanism comprises a dye liquor pipe and an air bag. The cerebrospinal fluid sample inspection device is used for solving the problems that in the prior art, the cerebrospinal fluid sample is only partially effective due to overlong time for inspection, and judgment of an inspection result is influenced.
Description
Technical Field
The invention belongs to the technical field of cytological examination sample processing devices, and particularly relates to an integrated cell processing device.
Background
The cerebrospinal fluid is colorless and transparent liquid and is filled in each ventricle, subarachnoid space and spinal cord central canal. Cerebrospinal fluid is produced by the choroid plexus in the ventricles, and is somewhat viscous, similar in nature to plasma and lymphatic fluid. Cerebrospinal fluid is continuously produced and continuously absorbed and returned to veins, plays the role of lymph fluid in the central nervous system, supplies certain nutrition to brain cells, transports metabolites of brain tissues and regulates the acid-base balance of the central nervous system. And the pressure of the brain and the spinal cord is buffered, and the brain and the spinal cord are protected and supported.
Cerebrospinal fluid (CSF) is produced by the intracerebroventricular choroid plexus and covers the entire brain and spinal cord surface. Because it is closest to the brain, spinal cord and nerves, when the brain, meninges, spinal cord and nerves are diseased, the corresponding pathological changes can be produced earlier in the cerebrospinal fluid. Therefore, cerebrospinal fluid examination has very important and unique application value for diagnosis, differential diagnosis, treatment guidance, curative effect observation, prognosis judgment and the like of nervous system, especially central nervous system infectious diseases.
At present, most of clinicians collect patient cerebrospinal fluid, and brain fluid specimen is collected in the sample test tube and then is submitted for inspection, and the doctor of the clinical laboratory carries out the preliminary treatment to the cerebrospinal fluid specimen, and the preliminary treatment includes collecting, flaking, fixing and dyeing the cell, observes through the microscope at last, reachs the test result. However, the test result has high requirement on the timeliness of the cerebrospinal fluid specimen, and if the inspection time is too long and exceeds 2 hours or the inspection is overflowed, the test result has great influence on the cerebrospinal fluid test result. The pretreatment of the clinical laboratory doctors requires a lot of time, and especially under the conditions of excessive samples to be submitted and compact physician resources, the cerebrospinal fluid samples are only partially effective, which affects the judgment of the test results.
Disclosure of Invention
In view of the above, the present invention provides an integrated cell processing device, which solves the problem in the prior art that the cerebrospinal fluid sample is only partially effective due to too long time for submitting the cerebrospinal fluid sample, thereby affecting the judgment of the test result.
The invention solves the technical problems by the following technical means:
an integrated cell processing device, comprises a shell, a centrifugal mechanism, a sheet making mechanism and a dyeing mechanism, wherein the centrifugal mechanism, the sheet making mechanism and the dyeing mechanism are arranged in the shell, the shell comprises an upper shell, a lower shell and a top cover, one end of the upper shell is fixedly connected with the lower shell, the other end of the upper shell is fixedly connected with the top cover, the centrifugal mechanism comprises a driving motor and a centrifugal cylinder, a centrifugal tube is arranged in the centrifugal cylinder, the centrifugal tube is detachably arranged in the centrifugal cylinder, a containing cavity is arranged in the upper shell, a centrifugal groove and a motor groove are formed in the bottom of the containing cavity, the centrifugal cylinder is inserted in the motor groove, one end of the centrifugal cylinder is arranged in the centrifugal groove through a bearing, the other end of the centrifugal cylinder is rotatably connected with the top cover through a bearing, the driving motor is fixedly arranged in the motor groove, a gear is fixedly, the gear meshes with first ring gear, the film-making mechanism is including putting a rack and multi-disc slide glass, it sets up to put a rack slope, the slide glass is placed on putting a rack, it rotates to set up in the inferior valve body to put a rack, dyeing mechanism includes dye liquor pipe and gasbag, hold the chamber bottom and seted up the dyeing tank, the dyeing pipe passes the top cap and installs in the dyeing tank, the gasbag is located outside the top cap to with dye liquor pipe fixed connection.
Further, the centrifuging tube includes tube head, pipe shaft and tube cap, first connecting groove has been seted up at the top of centrifuge tube, threaded connection is in first connecting groove under the tube cap, the tube cap overcoat is equipped with presses the shell, press shell and tube cap threaded connection, just terminal surface offsets with the centrifuge tube under the shell.
Further, the bottom of the pipe cover is provided with a second connecting groove, the pipe body is in threaded connection with the second connecting groove, a first piston ball is arranged in the tube body and is tightly connected with the inner wall of the tube body, a pressing cavity is arranged on the tube cover, a piston is arranged in the pressing cavity, the piston is hermetically connected with the side wall of the pressing cavity, the top end of the pressing cavity is provided with a through hole, the piston is integrally formed with a connecting column, the connecting column passes through the through hole to be fixedly connected with the pressing shell, an air inlet groove is formed in the piston, the air inlet groove penetrates through the connecting column and the pressing shell, a first one-way valve is arranged at the end of the piston in the air inlet groove, press the chamber lower extreme to install the second check valve, the mounting groove has been seted up at the tube cap top, install the spring in the installation, spring one end and mounting groove bottom fixed connection, the other end with press shell fixed connection.
Further, pipe shaft and tube head threaded connection, the tube head bottom is equipped with the rubber closing cap, redundant groove has been seted up to the centrifuge slot bottom, redundant groove runs through the casing bottom surface, it has first spacing ring to go up casing bottom surface in redundant groove department integrated into one piece, redundant inslot installs the water dropper, integrated into one piece has the second spacing ring on the water dropper, the water dropper upper end is equipped with the puncture head, be equipped with the barb on the puncture head, the water dropper lower extreme is equipped with first drip nozzle, a plurality of notches have been seted up on the puncture head, integrated into one piece has a plurality of first spacing strips on the inside wall of pressing the shell, integrated into one piece has a plurality of second spacing strips on the lateral wall of tube cap upper end.
Further, a plurality of fixing plates which are uniformly distributed are arranged on the sheet placing frame, a hinged seat is arranged at the upper end of the sheet placing frame, the hinge seat is hinged with a hinge joint which is rotationally connected with the hinge seat through a torsional spring, a sliding rod is fixedly connected to the hinged joint, a sliding hole is formed in the fixing plate, the sliding rod is inserted into the sliding hole, the tail end of the sliding rod is fixedly connected with a third spring, one end of the third spring is fixedly connected with the sliding rod, the other end of the third spring is fixedly connected in the sliding hole, the fixed plate is integrally formed with a limit shell at the hinged end, the end of the fixed plate is hinged with a limit block, one end of the glass slide is clamped in the limiting shell, the other end of the glass slide is abutted against the limiting block, the bottom of the fixing plate is provided with a magnetic sheet, the wafer placing frame is provided with an adsorption area, the side wall of the lower shell is provided with an observation window, and a cover body is arranged at the observation window.
Furthermore, the bottom of the sheet placing frame is provided with an annular groove, a second toothed ring is arranged on one side of the annular groove of the sheet placing frame, the side wall of the lower shell is provided with a plurality of driving grooves, a third toothed ring is rotatably arranged in the driving grooves, one end of the third toothed ring is positioned on the outer side of the lower shell, and the other end of the third toothed ring is meshed with the second toothed ring.
Further, a cavity is arranged in the sheet placing frame, a drying mechanism is arranged in the cavity and comprises a heating coil and a support, the heating coil is mounted at the top end of the support, and a heat dissipation groove is formed in the side wall of the cavity.
Further, dyeing mechanism is equipped with two sets ofly, be equipped with the second piston ball in the dye liquor pipe, the second piston ball closely meets with dyeing pipe inner wall, the one end of gasbag is equipped with the third check valve, and the other end is equipped with the fourth check valve, dyeing pipe bottom is equipped with the second mouth of dripping.
Further, still be equipped with in the upper shell and wash the mechanism, wash the mechanism with dyeing mechanism structure is the same, it has seted up in the cavity below and has collected the chamber to put the piece frame, the upper end of collecting the chamber lateral wall has seted up the chute, the chute runs through and puts a frame lateral wall, the bottom of collecting the chamber has seted up the weeping groove, the weeping hole has been seted up to casing bottom down, weeping groove and weeping hole intercommunication, the external chock plug that is equipped with of lower casing, chock plug sealing connection is in the weeping hole.
The invention has the beneficial effects that:
on the one hand, through setting up centrifugation mechanism, film-making mechanism and dyeing mechanism and setting up in an organic whole, clinician's portability this device after getting a cerebrospinal fluid sample, can carry out a series of operations such as centrifugation, film-making dyeing through this device, direct clinical slide censorship of making. The cerebrospinal fluid sample does not need to be checked again, so that the timeliness of the cerebrospinal fluid sample is prevented from being influenced by overlong checking time; on the other hand, through the drying mechanism who sets up, can make quick fixed of cell to avoid the cell to slide from the slide, and by the dyeing wash.
Drawings
FIG. 1 is a schematic view of an integrated cell processing apparatus according to the present invention;
FIG. 2 is a schematic cross-sectional view of an integrated cell processing apparatus according to the present invention;
FIG. 3 is a schematic sectional view showing a structure of a centrifugal tube in the integrated cell processing apparatus according to the present invention;
FIG. 4 is a schematic diagram showing a first structure of the integrated cell processing apparatus according to the present invention;
FIG. 5 is a schematic diagram showing a second split structure of the upper housing of the integrated cell processing device according to the present invention;
FIG. 6 is a schematic cross-sectional view of a slide groove in an integrated cell processing apparatus according to the present invention;
FIG. 7 is a schematic view showing a disassembled structure of a fixing plate and a slide glass in an integrated cell processing device according to the present invention.
Wherein, the upper shell 11, the containing cavity 111, the centrifugal groove 112, the motor groove 113, the redundant groove 114, the first limit ring 115, the dyeing groove 116, the lower shell 12, the observation window 121, the cover 122, the third gear ring 123, the top cover 13, the driving motor 21, the gear 211, the centrifugal cylinder 22, the first gear ring 221, the first connecting groove 222, the tube head 23, the rubber sealing cover 231, the tube body 24, the first piston ball 241, the tube cover 25, the second connecting groove 251, the pressing cavity 252, the mounting groove 253, the second limit strip 254, the pressing shell 26, the piston 261, the connecting column 262, the air inlet groove 263, the first limit strip 264, the spring 27, the dropper 3, the second limit ring 31, the puncture head 32, 321, the barb 322, the first drip nozzle 33, the sheet holder 41, the hinged seat 411, the cavity 412, the heat dissipation groove 413, the collection slide cavity 414, the chute 415, the slide glass 42, the fixing plate 43, the limit shell 431, the limit block 432, the slide hole 433, 434, heating coil 51, bracket 52, dye liquor pipe 61, air bag 62, second piston ball 611, hinged joint 71, sliding rod 711, third spring 712 and flushing mechanism 8.
Detailed Description
The invention will be described in detail below with reference to the following figures and specific examples:
as shown in FIGS. 1 to 7, an integrated cell processing device of the present invention comprises a housing, and a centrifugation mechanism, a slide-making mechanism and a staining mechanism installed in the housing. The casing includes upper housing 11, lower casing 12 and top cap 13, and upper housing 11 one end and lower casing 12 fixed connection, the other end and top cap 13 fixed connection.
The centrifugal mechanism comprises a driving motor 21 and a centrifugal cylinder 22, a centrifugal tube is arranged in the centrifugal cylinder 22, and the centrifugal tube is detachably arranged in the centrifugal cylinder 22. The upper shell 11 is provided with a containing cavity 111, the bottom of the containing cavity 111 is provided with a centrifugal groove 112 and a motor groove 113, the centrifugal cylinder 22 is inserted into the motor groove 113, one end of the centrifugal cylinder 22 is installed in the centrifugal groove 112 through a bearing, so that the centrifugal cylinder 22 is rotatably connected in the centrifugal groove 112, and the other end of the centrifugal cylinder 22 is rotatably connected with the top cover 13 through a bearing, so as to prevent the centrifugal cylinder 22 from jumping. The driving motor 21 is fixedly installed in the motor groove 113, the output shaft of the driving motor 21 is fixedly connected with a gear 211, the outer wall of the centrifugal cylinder 22 is integrally formed with a first gear ring 221, and the gear 211 is meshed with the first gear ring 221. When the centrifugal tube is used, cerebrospinal fluid collected by a clinician is moved into the centrifugal tube, and when the driving motor 21 works, the centrifugal tube 22 can be driven to rotate to centrifuge the cerebrospinal fluid in the centrifugal tube, so that cells in the cerebrospinal fluid are precipitated at the bottom of the centrifugal tube.
The centrifuging tube includes tube head 23, pipe shaft 24 and tube cap 25, first connecting groove 222 has been seted up at the top of centrifuge tube 22, threaded connection is in first connecting groove 222 under the tube cap 25, tube cap 25 overcoat is equipped with presses shell 26, press shell 26 and tube cap 25 threaded connection, and press shell 26 down the terminal surface and offset with centrifuge tube 22, make tube cap 25 and centrifuge tube 22 be connected firmly, when avoiding centrifuge tube 22 to rotate, take place relative rotation between centrifuge tube 22 and the centrifuging tube.
The bottom of the tube cover 25 is provided with a second connecting groove 251, and the tube body 24 is screwed into the second connecting groove 251, so that the centrifuge tube and the centrifuge tube 22 are firmly connected. Be equipped with first piston ball 241 in the pipe shaft 24, first piston ball 241 closely meets with the inner wall of pipe shaft 24, seted up on the tube cap 25 and pressed chamber 252, be equipped with piston 261 in pressing chamber 252, piston 261 and the lateral wall sealing connection who presses chamber 252, the through-hole has been seted up on the top of pressing chamber 252, integrated into one piece has spliced pole 262 on the piston 261, spliced pole 262 passes the through-hole and presses shell 26 fixed connection, the air inlet casing 263 has been seted up on the piston 261, air inlet casing 263 runs through spliced pole 262 and presses shell 26, first check valve is installed in piston 261 end in air inlet casing 263, press the chamber 252 lower extreme to install the second check valve, mounting groove 253 has been seted up at the tube cap 25 top, install spring 27 in the installation, spring 27 one end and mounting groove 253 bottom fixed connection, the other end and press shell 26 fixed connection.
According to integrated into one piece on the inside wall of shell 26 has a plurality of first spacing strips 264, integrated into one piece has a plurality of second spacing strips 254 on the lateral wall of tube cap 25 upper end, in the use, rotatory shell 26 of pressing, make shell 26 and tube cap 25 break away from threaded connection, under the effect of spring 27, make and press shell 26 to go up the bullet, first spacing strip 264 is gone into in the second spacing strip 254, rotate once more and press shell 26, make tube cap 25 rotate through first spacing strip 264 and second spacing strip 254, the centrifuging tube further twists into in the centrifuge tube 22, the centrifuging tube moves down, the puncture head 32 of bottom punctures rubber closing cap 231, enter into the centrifuging tube, communicate dropper 3 with the centrifuging tube through notch 321.
When extruding the cells in the centrifuge tube, the pressing shell 26 is pressed, the piston 261 moves downwards, the first one-way valve is closed, the second one-way valve is opened, the gas in the pressing cavity 252 is pushed into the centrifuge tube, the first piston ball 241 is forced to move downwards by the air pressure, the cells precipitated at the bottom of the centrifuge tube are extruded out of the centrifuge tube, enter the dripper 3 through the notch 321, and then drip from the first dripping nozzle 33 of the dripper 3. After one-time extrusion is completed, the pressing shell 26 drives the piston 261 to move upwards under the action of the spring 27, at this time, the first check valve is opened, the second check valve is closed, and external air enters the pressing cavity 252 so as to perform the next extrusion.
The puncture head 32 is provided with a barb 322, when the cerebrospinal fluid treatment is finished, the centrifuge tube can be taken out from the centrifuge tube 22, and the dripper 3 can be taken out together through the barb 322.
The slide making mechanism comprises a slide holder 41 and a plurality of slides 42, wherein the slides 42 are preferably sticky slides, and are more favorable for fixing cells on the slides 42. The slide holder 41 is obliquely arranged, the slide glass 42 is placed on the slide holder 41, the slide holder 41 is rotatably arranged in the lower shell 12, the slide holder 41 is rotated to enable the slide glass 42 to be positioned right below the first dripping nozzle 33, and when cells in the dripper 3 drip from the first dripping nozzle 33, the cells can just drip on the slide glass 42. And slide down under the force of gravity to lay on the slide 42. The slide rack 41 is further rotated so that the next slide 42 is positioned directly below the first nozzle 33 to pick up the dropped cells again.
It is equipped with a plurality of evenly distributed's fixed plate 43 on the rack 41 to put, the upper end of putting the rack 41 is equipped with articulated seat 411, articulated on the articulated seat have a hinge 71, hinge 71 passes through the torsional spring and is connected with articulated seat 411 rotation, fixedly connected with slide bar 711 on the hinge 71, has seted up slide opening 433 on the fixed plate 43, slide bar 711 inserts and locates in slide opening 433, the terminal fixedly connected with third spring 712 of slide bar 711, third spring 712 one end and slide bar 711 fixed connection, other end fixed connection is in slide opening 433, makes fixed plate 43 and hinge 71 sliding connection. And in a normal state, under the action of the torsion spring, the fixing plate 43 is in a horizontal state, so that cell staining is facilitated. The bottom of the fixing plate 43 is provided with a magnetic sheet 434, the cassette 41 is provided with an adsorption area, and the fixing plate 43 can be obliquely fixed on the cassette 41 by pressing the fixing plate 43. The fixed plate 43 is integrally formed with a limiting shell 431 at the hinged end, the end of the fixed plate 43 is hinged with a limiting block 432, one end of the glass slide 42 is clamped in the limiting shell 431, and the other end of the glass slide 42 abuts against the limiting block 432, so that the glass slide 42 is fixed on the fixed plate 43.
An observation window 121 is formed in the side wall of the lower shell 12, the observation window 121 corresponds to the positions of the centrifugal tube and the dye solution tube 61, a cover body 122 is arranged at the position of the observation window 121, and the observation window 121 can be blocked through the cover body 122. When the cells and the staining solution are dropped, they can be observed through the observation window 121 for operation. The fixing plate 43 can be pulled to drop cells on different positions of the glass slide 42, so that the cells are prevented from being dropped on the same position, cells are prevented from being overlapped, and observation is not facilitated. The viewing window 121 also serves to pick and place the slide 42.
The bottom of the wafer placing frame 41 is provided with an annular groove, one side of the annular groove of the wafer placing frame 41 is provided with a second toothed ring, the side wall of the lower shell 12 is provided with a plurality of driving grooves, a third toothed ring 123 is rotatably installed in each driving groove, one end of the third toothed ring 123 is located on the outer side of the lower shell 12, and the other end of the third toothed ring 123 is meshed with the second toothed ring. When the third ring gear 123 is toggled, the second ring gear can be rotated, which in turn rotates the slide rack 41 for adjusting the position of the slide 42.
A cavity 412 is arranged in the sheet placing frame 41, a drying mechanism is arranged in the cavity 412, the drying mechanism comprises a heating coil 51 and a bracket 52, the heating coil 51 is arranged at the top end of the bracket 52, a heat dissipation groove 413 is formed in the side wall of the cavity 412, and when the heating coil 51 works, the temperature in the lower shell 12 can be raised, so that on one hand, the slide glass 42 on the sheet placing frame 41 can be preheated; on the other hand, after the cells are spread on the slide glass 42, the cells are dried to fix the cells on the slide glass 42. After the cells are dropped on the slide glass 42, the observation window 121 is sealed by the cover 122, so that the heat emitted from the heat generating coil 51 is prevented from leaking. After the cells are dried, the cover 122 is opened to avoid the influence of the temperature in the lower shell 12 on the cell dyeing step.
Dyeing mechanism is equipped with two sets ofly, can set up different dye liquors respectively in two sets of dyeing mechanisms, and dyeing mechanism includes dye liquor pipe 61 and gasbag 62, holds the chamber 111 bottom and has seted up dyeing tank 116, and the dyeing pipe passes top cap 13 and installs in dyeing tank 116, and gasbag 62 is located outside top cap 13 to with dye liquor pipe 61 fixed connection. The dyeing liquid pipe 61 is internally provided with a second piston ball 611, the second piston ball 611 is tightly connected with the inner wall of the dyeing pipe, one end of the air bag 62 is provided with a third one-way valve, the other end is provided with a fourth one-way valve, and the bottom of the dyeing liquid pipe 61 is provided with a second drip nozzle. After the cells are fixed, the slide 42 is rotated under the second drop nozzle. The air bag 62 is pressed, the third one-way valve is closed, the fourth one-way valve is opened, the gas in the air bag 62 enters the staining solution pipe 61, the second piston ball 611 is pushed to move downwards, the staining solution in the staining solution pipe 61 is extruded from the second dripping nozzle and drips on the glass slide 42, and cells on the glass slide 42 are stained. After the air bag 62 is released, the air bag 62 recovers deformation, the third one-way valve is opened, the fourth one-way valve is closed, and outside air enters the air bag 62 so as to squeeze the air bag 62 next time.
The upper shell 11 is also internally provided with a washing mechanism 8, the washing mechanism 8 has the same structure as the dyeing mechanism, when the cell on the glass slide 42 is dyed, the glass slide 42 is rotated to the washing mechanism 8, the glass slide 42 is obliquely adsorbed on the slide holder 41, and the cell on the glass slide 42 is washed by the washing mechanism. Put the piece frame 41 and seted up collection chamber 414 in the cavity below, the upper end of collecting chamber 414 lateral wall has seted up chute 415, and chute 415 runs through and puts piece frame 41 lateral wall, and the weeping groove has been seted up to the bottom of collecting chamber 414, and the weeping hole has been seted up to lower casing bottom, and weeping groove and weeping hole intercommunication are equipped with the chock plug outside the lower casing, and chock plug sealing connection is in the weeping hole. Dye liquor, flushing liquor and the like enter the collecting cavity through the chute and flow out of the device through the liquor leaking groove and the liquor leaking hole.
When the integrated cell processing device is used, the third toothed ring 123 is shifted to rotate the slide holder 41, the cover body 122 is opened, the limiting block 432 is taken out by hand from the observation window 121, the glass slide 42 is clamped on the fixing plate 43, the limiting block 432 is released, the fixing plate 43 retracts into the lower shell 12 under the action of the third spring 712, and then the third toothed ring 123 is shifted to place the glass slides 42 one by one. Then, the cover 122 is closed, the drying mechanism is operated, and the heating coil 51 heats the temperature inside the lower case 12 to a desired temperature to preheat the slide 42.
The cerebrospinal fluid collected by the clinician is then transferred from the tip 23 end into the centrifuge tube, and the tip 23 is screwed onto the tube body 24, sealing the centrifuge tube. The centrifuge tube is fixedly connected with the centrifuge tube 22 by screwing the tube cover 25 into the first connecting groove 222. Then the driving motor 21 works to drive the centrifugal cylinder 22 to rotate, so as to centrifuge the cerebrospinal fluid in the centrifugal tube, and the cells in the cerebrospinal fluid are precipitated at the bottom of the centrifugal tube.
After the centrifugation is accomplished, rotatory shell 26 of pressing, make and press shell 26 and tube cap 25 to break away from threaded connection, under the effect of spring 27, press shell 26 to go up the bullet, first spacing 264 is gone into in the spacing 254 of second, rotate once more and press shell 26, make tube cap 25 rotate through first spacing 264 and the spacing 254 of second, the centrifuging tube is further twisted in centrifuge tube 22, the centrifuging tube moves down, the puncture head 32 of bottom punctures rubber closing cap 231, enter into in the centrifuging tube, communicate dripper 3 and centrifuging tube through notch 321.
The third toothed ring 123 is shifted to enable the slide holder 41 to rotate, and the slide glass 42 is observed from the observation window 121, so that the slide glass is positioned under the first dripping nozzle 33, the pressing shell 26 is squeezed, the piston 261 moves downwards, the first one-way valve is closed, the second one-way valve is opened, the gas in the pressing cavity 252 is pushed into the centrifugal tube, the first piston ball 241 is forced to move downwards by the air pressure, the cells deposited at the bottom of the centrifugal tube are squeezed out of the centrifugal tube and enter the dripping head 3 through the notch 321, then the cells are dripped onto the slide glass 42 below from the first dripping nozzle 33 of the dripping head 3, the limiting block 432 is extracted by hand, the fixing plate 43 is gradually pulled out, the cells are dripped onto different areas on the slide glass 42, the limiting block 432 is released, the fixing plate 43 is retracted into the lower shell 12 under the action of the third spring 712, and the cells are dripped onto each slide glass 42 in sequence.
After the drying mechanism dries and fixes the cells on the glass slide 42, the cover body 122 is completely opened to quickly dissipate heat in the lower shell 12, the third toothed ring 123 is shifted to enable the glass slide 42 to be positioned right below the second dripping nozzle, the air bag 62 is squeezed, the third one-way valve is closed, the fourth one-way valve is opened, gas in the air bag 62 enters the dyeing liquid pipe 61, the second piston ball 611 is pushed to move downwards, dyeing liquid in the dyeing liquid pipe 61 is squeezed out from the second dripping nozzle and drops on the glass slide 42, and the cells on the glass slide 42 are dyed. The cell stain is dropped onto each slide 42 in turn. After standing for a period of time, the slide glass 42 is obliquely adsorbed on the slide holder 41 through the observation window 121 of the washing mechanism 8, the dye solution on the slide glass 42 is continuously washed, and the slide glass 42 is taken out for inspection after the washing is finished.
The integrated cell processing device is not limited to processing cells in cerebrospinal fluid inspection, such as hydrothorax and ascites cytological examination, and can be used for processing cells when cell centrifugation, smear, staining and other steps are required.
Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims. The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.
Claims (9)
1. An integrated cell processing device, characterized in that: including the casing to and install centrifugal mechanism, film-making mechanism and dyeing machine in the casing, the casing includes casing, lower casing and top cap, go up casing one end and casing fixed connection down, the other end and top cap fixed connection, centrifugal mechanism includes driving motor and centrifuge bowl, be equipped with the centrifuging tube in the centrifuge bowl, centrifuging tube detachable installs in the centrifuge bowl, be equipped with the chamber of holding in the casing, centrifugal groove and motor groove have been seted up to the bottom in chamber of holding, the centrifuge bowl is inserted and is located the motor inslot, centrifuge bowl one end is installed in the centrifugal groove through the bearing, and the other end passes through the bearing and is connected with the top cap rotation, driving motor fixed mounting is in the motor inslot, fixedly connected with gear on driving motor's the output shaft, integrated into one piece has first ring gear on the outer wall of centrifuge bowl, gear and first ring gear meshing, the slide making mechanism is including putting a rack and multi-disc slide glass, it sets up to put the slope of rack, the slide glass is placed on putting the rack, it rotates and sets up in the inferior valve internally to put the rack, dyeing mechanism includes dye liquor pipe and gasbag, hold the chamber bottom and seted up the dyeing tank, the dyeing pipe passes the top cap and installs in the dyeing tank, the gasbag is located outside the top cap to with dye liquor pipe fixed connection.
2. An integrated cell processing device according to claim 1, wherein: the centrifuging tube includes tube head, pipe shaft and tube cap, first connecting slot has been seted up at the top of centrifuge tube, threaded connection is in first connecting slot under the tube cap, the tube cap overcoat is equipped with presses the shell, press shell and tube cap threaded connection, just press terminal surface and centrifuge tube offset under the shell.
3. An integrated cell processing device according to claim 2, wherein: the bottom of the pipe cover is provided with a second connecting groove, the pipe body is in threaded connection with the second connecting groove, a first piston ball is arranged in the tube body and is tightly connected with the inner wall of the tube body, a pressing cavity is arranged on the tube cover, a piston is arranged in the pressing cavity, the piston is hermetically connected with the side wall of the pressing cavity, the top end of the pressing cavity is provided with a through hole, the piston is integrally formed with a connecting column, the connecting column passes through the through hole to be fixedly connected with the pressing shell, an air inlet groove is formed in the piston, the air inlet groove penetrates through the connecting column and the pressing shell, a first one-way valve is arranged at the end of the piston in the air inlet groove, press the chamber lower extreme to install the second check valve, the mounting groove has been seted up at the tube cap top, install the spring in the installation, spring one end and mounting groove bottom fixed connection, the other end with press shell fixed connection.
4. An integrated cell processing device according to claim 3, wherein: pipe shaft and tube head threaded connection, the tube head bottom is equipped with the rubber closing cap, redundant groove has been seted up to the centrifuge bowl bottom, redundant groove runs through the casing bottom surface, it has first spacing ring to go up casing bottom surface in redundant groove department integrated into one piece, redundant inslot installs the water dropper, integrated into one piece has the second spacing ring on the water dropper, the water dropper upper end is equipped with the puncture head, be equipped with the barb on the puncture head, the water dropper lower extreme is equipped with first drip nozzle, a plurality of notches have been seted up on the puncture head, integrated into one piece has a plurality of first spacing strips on the inside wall of pressing the shell, integrated into one piece has a plurality of second spacing strips on the lateral wall of tube cap upper end.
5. An integrated cell processing device according to claim 4, wherein: a plurality of fixing plates which are uniformly distributed are arranged on the film placing frame, a hinge seat is arranged at the upper end of the film placing frame, the hinge seat is hinged with a hinge joint which is rotationally connected with the hinge seat through a torsional spring, a sliding rod is fixedly connected to the hinged joint, a sliding hole is formed in the fixing plate, the sliding rod is inserted into the sliding hole, the tail end of the sliding rod is fixedly connected with a third spring, one end of the third spring is fixedly connected with the sliding rod, the other end of the third spring is fixedly connected in the sliding hole, the fixed plate is integrally formed with a limit shell at the hinged end, the end of the fixed plate is hinged with a limit block, one end of the glass slide is clamped in the limiting shell, the other end of the glass slide is abutted against the limiting block, the bottom of the fixing plate is provided with a magnetic sheet, the wafer placing frame is provided with an adsorption area, the side wall of the lower shell is provided with an observation window, and a cover body is arranged at the observation window.
6. An integrated cell processing device according to claim 5, wherein: the bottom of the wafer placing frame is provided with a ring-shaped groove, a second toothed ring is arranged on one side of the ring-shaped groove of the wafer placing frame, the side wall of the lower shell is provided with a plurality of driving grooves, a third toothed ring is rotatably arranged in each driving groove, one end of each third toothed ring is located on the outer side of the lower shell, and the other end of each third toothed ring is meshed with the second toothed ring.
7. An integrated cell processing device according to claim 6, wherein: the improved wafer placing rack is characterized in that a cavity is arranged in the wafer placing rack, a drying mechanism is arranged in the cavity and comprises a heating coil and a support, the heating coil is installed at the top end of the support, and a heat dissipation groove is formed in the side wall of the cavity.
8. An integrated cell processing device according to claim 7, wherein: the dyeing mechanism is provided with two groups, a second piston ball is arranged in the dyeing liquid pipe, the second piston ball is tightly connected with the inner wall of the dyeing pipe, one end of the air bag is provided with a third one-way valve, the other end of the air bag is provided with a fourth one-way valve, and a second drip nozzle is arranged at the bottom of the dyeing liquid pipe.
9. An integrated cell processing device according to claim 8, wherein: the upper shell is internally provided with a washing mechanism, the washing mechanism is the same as the dyeing mechanism in structure, the sheet placing frame is arranged below the cavity and provided with a collecting cavity, the upper end of the side wall of the collecting cavity is provided with a chute, the chute penetrates through the side wall of the sheet placing frame, the bottom of the collecting cavity is provided with a liquid leakage groove, the bottom of the lower shell is provided with a liquid leakage hole, the liquid leakage groove is communicated with the liquid leakage hole, the lower shell is externally provided with a plug head, and the plug head is hermetically connected into the liquid leakage hole.
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Cited By (1)
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| CN117451457A (en) * | 2023-11-03 | 2024-01-26 | 青岛众精普汇医学科技有限公司 | Auxiliary diagnosis and detection device based on urothelial cancer |
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| CN112504796B (en) | 2024-06-25 |
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