CN112229932A - Method and kit for detecting multiple steroid hormones in blood sample - Google Patents
Method and kit for detecting multiple steroid hormones in blood sample Download PDFInfo
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- CN112229932A CN112229932A CN202011146551.0A CN202011146551A CN112229932A CN 112229932 A CN112229932 A CN 112229932A CN 202011146551 A CN202011146551 A CN 202011146551A CN 112229932 A CN112229932 A CN 112229932A
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- 239000003270 steroid hormone Substances 0.000 title claims abstract description 36
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- 238000007789 sealing Methods 0.000 claims abstract description 71
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- 239000007924 injection Substances 0.000 claims abstract description 19
- 238000012360 testing method Methods 0.000 claims abstract description 5
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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Abstract
The invention provides a method and a kit for detecting multiple steroid hormones in a blood sample, which comprises a sealing device, the sealing device comprises a sealing device main body, an injection port, a magnetic sheet, an external thread, an electric heating belt and a sealing film, the injection opening is embedded in the upper end face of the sealing device main body, the magnetic sheets are fixedly connected to the periphery of the side end face of the sealing device main body, the external thread is arranged at the lower part of the side end surface of the sealing device main body, the electric heating belt is fixedly arranged on the lower end surface of the sealing device main body, the invention provides a method for testing steroid hormone in blood and a test kit, wherein the sealing film is adhered to the inner wall of a sealing device main body, the lower end surface of the sealing device is rotationally connected with a collecting device, the kit can be recycled to a certain extent, the detection cost is low, and the detection process is safer and more reliable.
Description
Technical Field
The invention relates to the field of detection, in particular to a method and a kit for detecting multiple steroid hormones in a blood sample.
Background
Steroid hormones are also known as steroid hormones. Has extremely important medical value. Has effects in maintaining life, regulating sexual function, and promoting development of body, regulating immunity, treating skin diseases, and controlling fertility. The discovery and development of steroid hormone drugs is an important stage in the development of the medicinal chemistry discipline.
Meanwhile, steroid hormones, as a typical environmental endocrine disrupter, have strong endocrine disrupting effects and great harm to ecology and environment, are mainly discharged from vertebrates including human beings, and are continuously detected in the environment at present.
Meanwhile, steroid hormones are also important indexes reflecting the physical state of people, and chromatographic analysis is one of the most effective methods for monitoring steroid substances at present, and chromatographic analysis refers to a method for separating and analyzing according to the difference of distribution coefficients of substances between a fixed phase and a mobile phase. It can be classified into liquid chromatography, gas chromatography, supercritical fluid chromatography, etc. according to the molecular aggregation state of the mobile phase. Separation principles can be classified into various categories such as adsorption, partition, steric exclusion, ion exchange, affinity and chiral chromatography. According to the operating principle, the method can be classified into column chromatography, plate chromatography and the like.
However, a detection kit for steroid hormones in blood is lacked in the existing market, and after blood is collected, a corresponding sample is transferred to a corresponding detection instrument, so that the operation process is complicated, the sample is easy to pollute or pollute the environment and an operator, and the actual operation cost is high.
Disclosure of Invention
Technical problem to be solved
In view of the deficiencies of the prior art, the present invention provides a method and a kit for detecting multiple steroid hormones in a blood sample, which solves the problems set forth in the background art.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a detect multiple steroid hormone kit in blood sample, includes sealing device, sealing device includes sealing device main part, filling opening, magnetic force piece, external screw thread, electric heat tape and sealing film, the filling opening inlays the up end of establishing in sealing device main part, magnetic force piece fixed connection is around the side end face of sealing device main part, the side end face lower part in the sealing device main part is seted up to the external screw thread, electric heat tape fixed mounting is at the lower terminal surface of sealing device main part, the inner wall of sealing film adhesion at the sealing device main part, sealing device's lower terminal surface rotates and is connected with collection device, collection device includes go up go-between, internal screw thread, protective film, observation window, rotor plate, go up go-between, axis of rotation, filter tank, stationary blade, connecting thread, stirring ring, via hole and collection device main part, go up the up go-between fixed connection at the up end face of collection device main part, the inner thread inlays the inner wall of establishing at last go-between, the outer terminal surface at the collection device main part is seted up to the observation window, go-between fixed connection is at the lower terminal surface of collection device main part down, the inside fixedly connected with stationary blade of go-between down, the rotor plate rotates the up end of connecting at the stationary blade through the axis of rotation, the inside at the rotor plate is seted up to the via hole, the inside at the go-between down is seted up to the filter-tank, connecting thread inlays and establishes at go-between side end face lower part down, protective film covers the inner wall at collection device main part and last go-between, the lower terminal surface fixedly connected with chromatographic device of collection device, chromatographic device includes chromatogram matrix, fixing bolt, sense terminal and chromatographic column main part, the inside at chromatographic column main part is filled to the chromatogram matrix, and still is equipped with the filter-tank in chromatographic matrix's top, the sense terminal passes through fixing bolt fixed connection at the lower terminal A chromatographic window is arranged, and the side end surface of the chromatographic device is rotatably sleeved with a connecting ring.
Preferably, the upper part of the side end face of the lower connecting ring is provided with a groove matched with the poking ring, a movable groove is further formed in the groove, and the poking ring is fixedly connected with the side end face of the rotating plate through the movable groove.
Preferably, the outer surfaces of the poking ring and the collecting device are provided with limiting grooves matched with the easy-to-tear tapes.
Preferably, the external thread is matched with the internal thread, and the inner wall of the connecting ring is provided with a thread matched with the connecting thread.
Preferably, a hole site matched with the via hole is formed in the rotating plate.
Preferably, the chromatographic matrix is different according to the type of the steroid hormone to be detected, and at least one type of the chromatographic matrix is arranged.
Preferably, the injection port is made of a rubber material, and the injection port has at least one color for distinguishing different types of chromatographic matrixes.
Preferably, the collecting device body is made of paper or degradable foam materials.
Preferably, the observation windows are divided into two groups and symmetrically distributed on two sides of the side end face of the collecting device main body.
Also includes a method for detecting multiple steroid hormones in a blood sample: comprises the following steps:
the method comprises the following steps: selecting different types of detection kits according to the detection requirements of steroid hormones, and distinguishing the different types of detection kits through the color of an injection port;
step two: after the selection of the kit is finished, directly collecting a blood sample by using a blood taking needle, inserting one end of the blood taking needle into the injection opening, and collecting a small amount of blood;
step three: the blood collection state can be observed through the observation window during blood collection, and after the blood collection is finished, the kit can be adsorbed on the metal surface through the magnetic sheet for fixation and waits for chromatographic analysis;
step four: before chromatographic analysis, the easily torn strip on the surface of the collecting device needs to be torn, the toggle ring is toggled to enable the rotating plate to rotate, and when the via hole in the rotating plate is matched with the hole position formed in the fixing plate, internal blood flows into the filter tank under the action of gravity;
step five: the poking ring is pulled back to avoid backflow after the blood flows into the filter tank, the detection end is inserted into a corresponding chromatographic detection instrument to detect the chromatographic result of the blood sample, and a chromatographic window is arranged for observing the detection progress;
step six: the detection kit is recovered after the blood sample detection is finished, the sealing device, the collecting device and the chromatographic device are separated by rotating the sealing device and the connecting ring, the collecting device and the chromatographic device are replaced after the separation, the test can be continued by reassembling, and the collecting device and the chromatographic device are used for recycling.
(III) advantageous effects
The invention provides a method and a kit for detecting multiple steroid hormones in a blood sample, which have the following beneficial effects:
the invention designs a reusable and environment-friendly steroid hormone detection kit by combining a negative pressure vacuum blood collection tube and chromatographic column equipment, the kit can realize the detection of different types of steroid hormones by using a plurality of chromatographic matrixes, so that the detection reagent has wide detection range and more audiences, and the reagents of different types of chromatographic matrixes can be distinguished by adopting injection ports with different colors, meanwhile, the invention avoids the problems that the existing detection equipment adopts a plastic tube or a quartz glass tube more, is difficult to treat after being used and easily causes environmental pollution, a collection device body adopts paper or degradable materials, and a sealing device and the chromatographic device adopt a fixing mode of threaded connection, so that the kit can be detached and cleaned after being used, and a sealing film is adhered inside the sealing device body to avoid the pollution of a sample to the sealing device, the sealing film is heated by the electric heating tape and then is fixed and dissolved with the protective film, the sealing film is driven to be separated and replaced when the collecting device falls off, further the pollution of a blood sample to the collecting device is avoided, only the sealing film is required to be adhered inside the sealing device again, meanwhile, magnetic sheets are arranged on the periphery of the side end surface of the main body of the sealing device and can be used for being inverted and supported and also can be used for being adsorbed on a metal surface for fixing, the trouble that the existing kit needs to be supported by a special fixing support is avoided, and the chromatographic device can still be used for two times after being subjected to special processing and cleaning under the condition that a chromatographic substrate is not damaged after being used, the detection cost of the kit is greatly improved, meanwhile, the easy-tearing tape is arranged on the surface of the collecting device, the shifting ring is limited and fixed through the easy-tearing tape when the collecting device is not used, further the vacuum tightness of the inside of the, and the easy-to-tear tape adopts an embedded design, so that the kit failure caused by manual misoperation is avoided, and the design is more humanized.
Drawings
FIG. 1 is a schematic view of the overall structure of the apparatus of the present invention;
FIG. 2 is a schematic view of the sealing device of the present invention;
FIG. 3 is a schematic sectional view of the kit of the present invention;
FIG. 4 is a schematic cross-sectional view of a chromatographic apparatus of the present invention.
In the figure: 1 sealing device, 2 collecting device, 3 easy-to-tear tape, 4 chromatographic window, 5 chromatographic device, 6 connecting rings, 7 sealing device main body, 8 injection port, 9 magnetic force sheet, 10 external thread, 11 electric heating tape, 12 sealing film, 13 upper connecting ring, 14 internal thread, 15 protective film, 16 observation window, 17 rotating plate, 18 lower connecting ring, 19 rotating shaft, 20 filtering tank, 21 fixing sheet, 22 connecting thread, 23 stirring ring, 24 through hole, 25 collecting device main body, 26 chromatographic matrix, 27 fixing bolt, 28 detection end and 29 chromatographic column main body.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
The following disclosure provides many different embodiments or examples for implementing different features of the invention. To simplify the disclosure of the present invention, the components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit the present invention. Furthermore, the present invention may repeat reference numerals and/or letters in the various examples, such repetition is for the purpose of simplicity and clarity and does not in itself dictate a relationship between the various embodiments and/or configurations discussed. In addition, the present invention provides examples of various specific processes and materials, but one of ordinary skill in the art may recognize applications of other processes and/or uses of other materials.
The embodiment of the invention provides a kit for detecting multiple steroid hormones in a blood sample, which comprises a sealing device 1, wherein the sealing device 1 comprises a sealing device main body 7, an injection port 8, a magnetic sheet 9, an external thread 10, an electric heating belt 11 and a sealing film 12, the injection port 8 is embedded on the upper end face of the sealing device main body 7, the magnetic sheet 9 is fixedly connected to the periphery of the side end face of the sealing device main body 7, the external thread 10 is arranged at the lower part of the side end face of the sealing device main body 7, the electric heating belt 11 is fixedly arranged on the lower end face of the sealing device main body 7, the sealing film 12 is adhered to the inner wall of the sealing device main body 7, the lower end face of the sealing device 1 is rotatably connected with a collecting device 2, and the collecting device 2 comprises an upper connecting ring 13, an internal thread 14, a protective film 15, an observation window 16, a rotating plate 17, a lower connecting, The device comprises a dial ring 23, a via hole 24 and a collecting device body 25, wherein an upper connecting ring 13 is fixedly connected on the upper end face of the collecting device body 25, an internal thread 14 is embedded on the inner wall of the upper connecting ring 13, an observation window 16 is arranged on the outer end face of the collecting device body 25, a lower connecting ring 18 is fixedly connected on the lower end face of the collecting device body 25, a fixing piece 21 is fixedly connected inside the lower connecting ring 18, a rotating plate 17 is rotatably connected on the upper end face of the fixing piece 21 through a rotating shaft 19, a via hole 24 is arranged inside the rotating plate 17, a filter tank 20 is arranged inside the lower connecting ring 18, a connecting thread 22 is embedded on the lower portion of the side end face of the lower connecting ring 18, a protective film 15 covers on the inner walls of the collecting device body 25 and the upper connecting ring 13, a chromatographic device 5 is fixedly connected on the lower end face of the collecting device 2, the chromatographic, the chromatographic matrix 26 is filled in the chromatographic column main body 29, the filter tank 20 is further arranged above the chromatographic matrix 26, the detection end 28 is fixedly connected to the front end face of the chromatographic device 5 at the lower end face of the chromatographic column main body 29 through the fixing bolt 27, the chromatographic window 4 is opened on the front end face, and the connecting ring 6 is rotatably sleeved on the side end face of the chromatographic device 5.
The upper part of the side end surface of the lower connecting ring 18 is provided with a groove matched with the toggle ring 23, a movable groove is also arranged in the groove, the toggle ring 23 is fixedly connected with the side end surface of the rotating plate 17 through the movable groove, the outer surfaces of the toggle ring 23 and the collecting device 2 are provided with a limit groove matched with the easy-to-tear tape 3, the external thread 10 is matched with the internal thread 14, the inner wall of the connecting ring 6 is provided with a thread matched with the connecting thread 22, the rotating plate 17 is internally provided with a hole site matched with the through hole 24, the chromatographic matrix 26 is provided with at least one type according to the different types of the steroid hormones to be detected, the injection opening 8 is made of rubber material, the injection port 8 has at least one color for distinguishing different types of chromatographic substrates 26, the collecting device body 25 is made of paper or degradable foam materials, and the two groups of observation windows 16 are symmetrically distributed on two sides of the side end face of the collecting device body 25.
Also includes a method for detecting multiple steroid hormones in a blood sample: comprises the following steps:
the method comprises the following steps: selecting different types of detection kits according to the detection requirements of the steroid hormones, wherein the different types of detection kits are distinguished through the color of the injection port 8;
step two: after the selection of the kit is finished, directly collecting a blood sample by using a blood taking needle, inserting one end of the blood taking needle into the injection opening 8, and collecting a small amount of blood;
step three: the blood collection state can be observed through the observation window 16 during blood collection, and after the blood collection is finished, the kit can be adsorbed on the metal surface through the magnetic sheet 9 for fixation and waits for chromatographic analysis;
step four: before chromatographic analysis, the easily torn strip 3 on the surface of the collecting device 2 needs to be torn, the poking ring 23 is poked to enable the rotating plate 17 to rotate, and when the through hole 24 in the rotating plate 17 is matched with the hole site formed in the fixing plate 21, the internal blood flows into the filter tank 20 under the action of gravity;
step five: the poking ring 23 is poked back to avoid backflow after the blood flows into the filtering tank 20, the detection end 28 is inserted into a corresponding chromatographic detection instrument to detect the chromatographic result of the blood sample, and a chromatographic window 4 is arranged for observing the detection progress;
step six: the detection kit is recovered after the blood sample detection is finished, the sealing device 1, the collecting device 2 and the chromatographic device 5 are separated by rotating the sealing device 1 and the connecting ring 6, the collecting device 2 and the chromatographic device 5 are replaced after the separation, the test can be continued by reassembling, and the collecting device 2 and the chromatographic device 5 are used for recycling.
In conclusion, the reusable and environment-friendly steroid hormone detection kit is designed by combining the negative pressure vacuum blood collection tube with the chromatographic column device, the kit can realize the detection of different steroid hormones by using a plurality of chromatographic substrates 26, so that the detection reagent has wide detection range and more audiences, the reagents of different chromatographic substrates 26 can be distinguished by adopting injection ports 8 with different colors, meanwhile, the invention avoids the problems that the existing detection device adopts a plastic tube or a quartz glass tube more and is difficult to treat after being used and easy to cause environmental pollution, the collection device main body 25 adopts paper or degradable materials, the sealing device 1 and the chromatographic device 5 adopt a fixing mode of threaded connection, so that the kit can be detached and cleaned after being used, and meanwhile, the sealing film 12 is adhered inside the sealing device main body 7 in order to avoid the pollution of a sample to the sealing device 1, the sealing film 12 is heated by the electric heating belt 11 and then is fixed and dissolved with the protective film 15, the sealing film 12 is driven to be separated from and replaced together when the collecting device 2 falls off, further the pollution of a blood sample to the collecting device 2 is avoided, only the sealing film 12 is required to be adhered to the inside of the sealing device 1 again, meanwhile, magnetic sheets 9 are arranged on the periphery of the side end surface of the sealing device main body 7, the magnetic sheets 9 can be used for inverted support and can also be used for being adsorbed on the metal surface for fixing, the trouble that the existing kit needs to be fixed by means of a special fixing support is avoided, the chromatographic device 5 is used under the condition that a chromatographic substrate 26 is not damaged, secondary use can be carried out after special processing and cleaning, the detection cost of the kit is greatly improved, meanwhile, the easy-tearing belt 3 is arranged on the surface of the collecting device 2, the shifting ring 23 is limited and fixed through the easy-tearing belt 3 when the collecting device 2 is not used, and further the internal vacuum sealing of the And the easy-to-tear tape 3 adopts an embedded design, so that the kit failure caused by manual misoperation is avoided, and the design is more humanized.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A kit for the detection of multiple steroid hormones in a blood sample, comprising a sealing device (1), characterized in that: sealing device (1) includes sealing device main part (7), filling opening (8), magnetic force piece (9), external screw thread (10), electric heat tape (11) and sealed film (12), filling opening (8) are inlayed and are established the up end in sealing device main part (7), magnetic force piece (9) fixed connection is around the side end face of sealing device main part (7), the side end face lower part in sealing device main part (7) is seted up in external screw thread (10), electric heat tape (11) fixed mounting is at the lower terminal surface of sealing device main part (7), sealed film (12) adhesion is at the inner wall of sealing device main part (7), the lower terminal surface of sealing device (1) rotates and is connected with collection device (2), collection device (2) include go up go-between (13), internal thread (14), protection film (15), observation window (16), Rotor plate (17), go up go-between (18), axis of rotation (19), filter-tank (20), stationary blade (21), connecting thread (22), stir ring (23), via hole (24) and collection device main part (25), go up go-between (13) fixed connection at the up end of collection device main part (25), internal thread (14) are inlayed and are established at the inner wall of last go-between (13), observe the outer terminal surface of window (16) seting up in collection device main part (25), go-between (18) fixed connection is at the lower terminal surface of collection device main part (25) down, the inside fixedly connected with stationary blade (21) of lower go-between (18), rotor plate (17) rotate through axis of rotation (19) and connect the up end at stationary blade (21), the inside at rotor plate (17) is seted up in via hole (24), the inside at lower go-between (18) is seted up in filter-tank (20), the connecting screw thread (22) is embedded at the lower part of the side end surface of the lower connecting ring (18), the protective film (15) covers the inner walls of the collecting device main body (25) and the upper connecting ring (13), the lower end face of the collecting device (2) is fixedly connected with a chromatographic device (5), the chromatographic device (5) comprises a chromatographic matrix (26), a fixing bolt (27), a detection end (28) and a chromatographic column main body (29), the chromatography matrix (26) is packed inside a chromatography column body (29), a filter tank (20) is arranged above the chromatographic matrix (26), the detection end (28) is fixedly connected to the lower end surface of the chromatographic column main body (29) through a fixing bolt (27), the front end surface of the chromatographic device (5) is provided with a chromatographic window (4), and the side end surface of the chromatographic device (5) is rotatably sleeved with a connecting ring (6).
2. The kit for detecting multiple steroid hormones in a blood sample as claimed in claim 1, wherein: the upper part of the side end face of the lower connecting ring (18) is provided with a groove matched with the poking ring (23), a movable groove is further formed in the groove, and the poking ring (23) is fixedly connected with the side end face of the rotating plate (17) through the movable groove.
3. The kit for detecting multiple steroid hormones in a blood sample as claimed in claim 2, wherein: the outer surfaces of the poking ring (23) and the collecting device (2) are provided with limiting grooves matched with the easy-to-tear tapes (3).
4. The kit for detecting multiple steroid hormones in a blood sample as claimed in claim 1, wherein: the external thread (10) is matched with the internal thread (14), and the inner wall of the connecting ring (6) is provided with a thread matched with the connecting thread (22).
5. The kit for detecting multiple steroid hormones in a blood sample as claimed in claim 1, wherein: and hole sites matched with the via holes (24) are formed in the rotating plate (17).
6. The kit for detecting multiple steroid hormones in a blood sample as claimed in claim 1, wherein: the chromatographic matrix (26) is different according to the type of the steroid hormone to be detected, and at least one type of the chromatographic matrix (26) is arranged.
7. The kit of claim 6, wherein said kit comprises: the injection port (8) is in particular of a rubber material, and the injection port (8) has at least one colour for distinguishing between different types of chromatographic matrix (26).
8. The kit for detecting multiple steroid hormones in a blood sample as claimed in claim 1, wherein: the collecting device main body (25) is made of paper or degradable foam materials.
9. The kit for detecting multiple steroid hormones in a blood sample as claimed in claim 1, wherein: the observation windows (16) are divided into two groups and are symmetrically distributed on two sides of the side end surface of the collecting device main body (25).
10. A method for detecting multiple steroid hormones in a blood sample comprises the following steps: the method is characterized by comprising the following steps:
the method comprises the following steps: selecting different types of detection kits according to the detection requirements of the steroid hormones, wherein the different types of detection kits are distinguished through the color of the injection port (8);
step two: after the selection of the kit is finished, directly collecting a blood sample by using a blood taking needle, inserting one end of the blood taking needle into the injection opening (8) and collecting a small amount of blood;
step three: the blood collection state can be observed through an observation window (16) during blood collection, and after the blood collection is finished, the kit can be adsorbed on the metal surface through a magnetic sheet (9) to be fixed and wait for chromatographic analysis;
step four: before chromatographic analysis, the easily torn strip (3) on the surface of the collecting device (2) needs to be torn, the poking ring (23) is poked to enable the rotating plate (17) to rotate, and when the through hole (24) in the rotating plate (17) is matched with the hole position formed in the fixing piece (21), the internal blood flows into the filter tank (20) under the action of gravity;
step five: the poking ring (23) is poked back to avoid backflow after the blood flows into the filtering tank (20), the detection end (28) is inserted into a corresponding chromatographic detection instrument to detect the chromatographic result of the blood sample, and a chromatographic window (4) is arranged for observing the detection progress;
step six: the detection kit is recovered after the blood sample detection is finished, the sealing device (1), the collecting device (2) and the chromatographic device (5) are separated by rotating the sealing device (1) and the connecting ring (6), the collecting device (2) and the chromatographic device (5) are replaced after the separation, the test can be continued by reassembling, and the collecting device (2) and the chromatographic device (5) are used for recovering and reusing.
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| CN202011146551.0A CN112229932A (en) | 2020-10-22 | 2020-10-22 | Method and kit for detecting multiple steroid hormones in blood sample |
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| CN202011146551.0A CN112229932A (en) | 2020-10-22 | 2020-10-22 | Method and kit for detecting multiple steroid hormones in blood sample |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114487210A (en) * | 2022-03-11 | 2022-05-13 | 天津国科医工科技发展有限公司 | Liquid chromatography tandem mass spectrometry detection method of steroid hormone based on magnetic solid phase extraction |
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| CN103752040A (en) * | 2014-01-07 | 2014-04-30 | 南京诺云生物科技有限公司 | Modularized chromatographic column and purposes thereof |
| KR101950372B1 (en) * | 2018-10-30 | 2019-02-20 | 한국화학연구원 | Diagnosis method for steroid metabolic profile, evaluating method of internet and smartphone addiction and computer-readable recording media using the same |
| CN213779983U (en) * | 2020-10-22 | 2021-07-23 | 青岛惠安康生物工程有限公司 | Kit for detecting multiple steroid hormones in blood sample |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103752040A (en) * | 2014-01-07 | 2014-04-30 | 南京诺云生物科技有限公司 | Modularized chromatographic column and purposes thereof |
| KR101950372B1 (en) * | 2018-10-30 | 2019-02-20 | 한국화학연구원 | Diagnosis method for steroid metabolic profile, evaluating method of internet and smartphone addiction and computer-readable recording media using the same |
| CN213779983U (en) * | 2020-10-22 | 2021-07-23 | 青岛惠安康生物工程有限公司 | Kit for detecting multiple steroid hormones in blood sample |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114487210A (en) * | 2022-03-11 | 2022-05-13 | 天津国科医工科技发展有限公司 | Liquid chromatography tandem mass spectrometry detection method of steroid hormone based on magnetic solid phase extraction |
| CN114487210B (en) * | 2022-03-11 | 2024-05-10 | 天津国科医疗科技发展有限公司 | Liquid chromatography tandem mass spectrometry detection method of steroid hormone based on magnetic solid phase extraction |
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