CN112437814A - 多核苷酸合成方法、试剂盒和系统 - Google Patents

多核苷酸合成方法、试剂盒和系统 Download PDF

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Publication number
CN112437814A
CN112437814A CN201980048280.4A CN201980048280A CN112437814A CN 112437814 A CN112437814 A CN 112437814A CN 201980048280 A CN201980048280 A CN 201980048280A CN 112437814 A CN112437814 A CN 112437814A
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China
Prior art keywords
nucleotide
strand
polynucleotide
cycle
nucleotides
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CN201980048280.4A
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English (en)
Chinese (zh)
Inventor
约翰·米尔顿
索比亚·纳亚
简·里德尔
大柿亮祐
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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Publication of CN112437814A publication Critical patent/CN112437814A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163—Biocompatibility

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980048280.4A 2018-07-19 2019-07-19 多核苷酸合成方法、试剂盒和系统 Pending CN112437814A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1811813.3A GB201811813D0 (en) 2018-07-19 2018-07-19 Method
GB1811813.3 2018-07-19
PCT/GB2019/052037 WO2020016606A1 (en) 2018-07-19 2019-07-19 Polynucleotide synthesis method, kit and system

Publications (1)

Publication Number Publication Date
CN112437814A true CN112437814A (zh) 2021-03-02

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Family Applications (1)

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CN201980048280.4A Pending CN112437814A (zh) 2018-07-19 2019-07-19 多核苷酸合成方法、试剂盒和系统

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US (2) US20220177938A1 (https=)
EP (1) EP3824101A1 (https=)
JP (1) JP7518812B2 (https=)
CN (1) CN112437814A (https=)
AU (1) AU2019306778A1 (https=)
CA (1) CA3104746A1 (https=)
GB (1) GB201811813D0 (https=)
WO (1) WO2020016606A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
US20230117083A1 (en) * 2020-01-22 2023-04-20 Nuclera Nucleics Ltd. Methods of nucleic acid synthesis
GB202013102D0 (en) 2020-08-21 2020-10-07 Nuclera Nucleics Ltd Methods of nucleic acid synthesis
CN115997030A (zh) 2020-04-30 2023-04-21 德迈逊科技有限公司 用于大分子操作的装置和方法
GB202309649D0 (en) * 2023-06-27 2023-08-09 Oxford Nanopore Tech Plc Method

Citations (5)

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CN101680872A (zh) * 2007-04-13 2010-03-24 塞昆纳姆股份有限公司 序列比较分析方法和系统
US20130085073A1 (en) * 2011-09-29 2013-04-04 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nuclleic acids synthesis and sequencing
CN103502448A (zh) * 2010-11-12 2014-01-08 Gen9股份有限公司 核酸合成的方法和设备
CN104093890A (zh) * 2012-01-26 2014-10-08 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
US20170218416A1 (en) * 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna

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US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
ES2292270B1 (es) * 2004-04-14 2009-02-16 Oryzon Genomics, S.A. Procedimiento para detectar selectivamente acidos nucleicos con estructuras atipicas convertibles en muescas.
US8653832B2 (en) 2010-07-06 2014-02-18 Sharp Kabushiki Kaisha Array element circuit and active matrix device
US8828336B2 (en) 2011-02-02 2014-09-09 Sharp Kabushiki Kaisha Active matrix device
US9492824B2 (en) 2013-01-16 2016-11-15 Sharp Kabushiki Kaisha Efficient dilution method, including washing method for immunoassay
US9169573B2 (en) 2013-01-23 2015-10-27 Sharp Kabushiki Kaisha AM-EWOD device and method of driving with variable voltage AC driving
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
JP6448097B2 (ja) * 2013-10-15 2019-01-09 モレキュラー アセンブリーズ， インコーポレイテッド 核酸を合成するための方法および装置
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
EP3699283A1 (en) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
GB201502152D0 (en) 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
GB201503534D0 (en) 2015-03-03 2015-04-15 Nuclera Nucleics Ltd Novel method
GB201512372D0 (en) 2015-07-15 2015-08-19 Nuclera Nucleics Ltd Novel method
WO2017223517A1 (en) * 2016-06-24 2017-12-28 The Regents Of The University Of California Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates
GB2559117B (en) * 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811811D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101680872A (zh) * 2007-04-13 2010-03-24 塞昆纳姆股份有限公司 序列比较分析方法和系统
CN103502448A (zh) * 2010-11-12 2014-01-08 Gen9股份有限公司 核酸合成的方法和设备
US20130085073A1 (en) * 2011-09-29 2013-04-04 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nuclleic acids synthesis and sequencing
CN104093890A (zh) * 2012-01-26 2014-10-08 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
US20170218416A1 (en) * 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna

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Publication number Publication date
GB201811813D0 (en) 2018-09-05
US20250263767A1 (en) 2025-08-21
CA3104746A1 (en) 2020-01-23
EP3824101A1 (en) 2021-05-26
WO2020016606A1 (en) 2020-01-23
JP7518812B2 (ja) 2024-07-18
JP2021529549A (ja) 2021-11-04
AU2019306778A1 (en) 2021-01-07
US20220177938A1 (en) 2022-06-09

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