JP7518812B2 - ポリヌクレオチドの合成法、キット、およびシステム - Google Patents

ポリヌクレオチドの合成法、キット、およびシステム Download PDF

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JP7518812B2
JP7518812B2 JP2021500956A JP2021500956A JP7518812B2 JP 7518812 B2 JP7518812 B2 JP 7518812B2 JP 2021500956 A JP2021500956 A JP 2021500956A JP 2021500956 A JP2021500956 A JP 2021500956A JP 7518812 B2 JP7518812 B2 JP 7518812B2
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nucleotide
strand
polynucleotide
cycle
synthesis
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JP2021529549A5 (https=
JP2021529549A (ja
Inventor
ミルトン，ジョン
ネイヤール，ソビア
リードル，ヤン
亮祐 大柿
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オックスフォード ナノポール テクノロジーズ ピーエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163—Biocompatibility

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021500956A 2018-07-19 2019-07-19 ポリヌクレオチドの合成法、キット、およびシステム Active JP7518812B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1811813.3A GB201811813D0 (en) 2018-07-19 2018-07-19 Method
GB1811813.3 2018-07-19
PCT/GB2019/052037 WO2020016606A1 (en) 2018-07-19 2019-07-19 Polynucleotide synthesis method, kit and system

Publications (3)

Publication Number Publication Date
JP2021529549A JP2021529549A (ja) 2021-11-04
JP2021529549A5 JP2021529549A5 (https=) 2022-06-30
JP7518812B2 true JP7518812B2 (ja) 2024-07-18

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JP2021500956A Active JP7518812B2 (ja) 2018-07-19 2019-07-19 ポリヌクレオチドの合成法、キット、およびシステム

Country Status (8)

Country Link
US (2) US20220177938A1 (https=)
EP (1) EP3824101A1 (https=)
JP (1) JP7518812B2 (https=)
CN (1) CN112437814A (https=)
AU (1) AU2019306778A1 (https=)
CA (1) CA3104746A1 (https=)
GB (1) GB201811813D0 (https=)
WO (1) WO2020016606A1 (https=)

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GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
US20230117083A1 (en) * 2020-01-22 2023-04-20 Nuclera Nucleics Ltd. Methods of nucleic acid synthesis
GB202013102D0 (en) 2020-08-21 2020-10-07 Nuclera Nucleics Ltd Methods of nucleic acid synthesis
CN115997030A (zh) 2020-04-30 2023-04-21 德迈逊科技有限公司 用于大分子操作的装置和方法
GB202309649D0 (en) * 2023-06-27 2023-08-09 Oxford Nanopore Tech Plc Method

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WO2001088173A2 (en) 2000-05-16 2001-11-22 Hercules Incorporated Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics
JP2007532120A (ja) 2004-04-14 2007-11-15 オリソン、ヘノミクス、ソシエダッド、アノニマ 核酸分子のサブセットを選択的に検出するための方法
US20130085073A1 (en) 2011-09-29 2013-04-04 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nuclleic acids synthesis and sequencing
JP2014504153A (ja) 2010-11-12 2014-02-20 ジェン９・インコーポレイテッド 核酸合成のための方法およびデバイス
JP2016515391A (ja) 2013-10-15 2016-05-30 モレキュラー アセンブリー， エルエルシー 核酸を合成するための方法および装置
WO2017223517A1 (en) 2016-06-24 2017-12-28 The Regents Of The University Of California Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates
JP2020506687A (ja) 2017-01-19 2020-03-05 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチド分子を合成するための方法および試薬
JP2021524246A (ja) 2018-05-23 2021-09-13 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチドの合成法、システム、およびキット
JP2021529548A (ja) 2018-07-19 2021-11-04 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチド合成方法、キット、およびシステム
JP2021529551A (ja) 2018-07-19 2021-11-04 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチドの合成法、キット、およびシステム

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US20090006002A1 (en) * 2007-04-13 2009-01-01 Sequenom, Inc. Comparative sequence analysis processes and systems
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US20170218416A1 (en) * 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
EP3699283A1 (en) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
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GB201512372D0 (en) 2015-07-15 2015-08-19 Nuclera Nucleics Ltd Novel method

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001088173A2 (en) 2000-05-16 2001-11-22 Hercules Incorporated Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics
JP2007532120A (ja) 2004-04-14 2007-11-15 オリソン、ヘノミクス、ソシエダッド、アノニマ 核酸分子のサブセットを選択的に検出するための方法
JP2014504153A (ja) 2010-11-12 2014-02-20 ジェン９・インコーポレイテッド 核酸合成のための方法およびデバイス
US20130085073A1 (en) 2011-09-29 2013-04-04 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nuclleic acids synthesis and sequencing
JP2016515391A (ja) 2013-10-15 2016-05-30 モレキュラー アセンブリー， エルエルシー 核酸を合成するための方法および装置
WO2017223517A1 (en) 2016-06-24 2017-12-28 The Regents Of The University Of California Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates
JP2020506687A (ja) 2017-01-19 2020-03-05 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチド分子を合成するための方法および試薬
JP2021524246A (ja) 2018-05-23 2021-09-13 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチドの合成法、システム、およびキット
JP2021529548A (ja) 2018-07-19 2021-11-04 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチド合成方法、キット、およびシステム
JP2021529551A (ja) 2018-07-19 2021-11-04 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチドの合成法、キット、およびシステム

Also Published As

Publication number Publication date
GB201811813D0 (en) 2018-09-05
CN112437814A (zh) 2021-03-02
US20250263767A1 (en) 2025-08-21
CA3104746A1 (en) 2020-01-23
EP3824101A1 (en) 2021-05-26
WO2020016606A1 (en) 2020-01-23
JP2021529549A (ja) 2021-11-04
AU2019306778A1 (en) 2021-01-07
US20220177938A1 (en) 2022-06-09

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