CN112424373A - 多核苷酸合成方法、试剂盒和系统 - Google Patents

多核苷酸合成方法、试剂盒和系统 Download PDF

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Publication number
CN112424373A
CN112424373A CN201980048279.1A CN201980048279A CN112424373A CN 112424373 A CN112424373 A CN 112424373A CN 201980048279 A CN201980048279 A CN 201980048279A CN 112424373 A CN112424373 A CN 112424373A
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China
Prior art keywords
nucleotide
strand
polynucleotide
cycle
nucleotides
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CN201980048279.1A
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English (en)
Chinese (zh)
Inventor
约翰·米尔顿
索比亚·纳亚
简·里德尔
大柿亮祐
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980048279.1A 2018-07-19 2019-07-19 多核苷酸合成方法、试剂盒和系统 Pending CN112424373A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1811810.9A GB201811810D0 (en) 2018-07-19 2018-07-19 Method
GB1811810.9 2018-07-19
PCT/GB2019/052035 WO2020016604A1 (en) 2018-07-19 2019-07-19 Polynucleotide synthesis method, kit and system

Publications (1)

Publication Number Publication Date
CN112424373A true CN112424373A (zh) 2021-02-26

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Family Applications (1)

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CN201980048279.1A Pending CN112424373A (zh) 2018-07-19 2019-07-19 多核苷酸合成方法、试剂盒和系统

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Country Link
US (1) US20210388432A1 (https=)
EP (1) EP3824100B1 (https=)
JP (1) JP7393412B2 (https=)
CN (1) CN112424373A (https=)
AU (1) AU2019304148A1 (https=)
CA (1) CA3104692A1 (https=)
GB (1) GB201811810D0 (https=)
WO (1) WO2020016604A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116087504A (zh) * 2023-02-24 2023-05-09 上海碧云天生物技术有限公司 免疫磁珠保护液、其制备方法及应用

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
KR102281359B1 (ko) * 2021-01-26 2021-07-22 씨제이제일제당 (주) 신규한 단백질 변이체 및 이를 이용한 l-발린 생산 방법
GB202309649D0 (en) * 2023-06-27 2023-08-09 Oxford Nanopore Tech Plc Method

Citations (5)

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US20030194736A1 (en) * 2002-04-12 2003-10-16 Jurate Bitinaite Methods and compositions for DNA manipulation
CN1860227A (zh) * 2003-10-02 2006-11-08 巴斯福股份公司 用于序列饱和诱变(SeSaM)的方法
US20110269631A1 (en) * 2007-09-14 2011-11-03 Affymetrix, Inc. Amplification and Analysis of Selected Targets on Solid Supports
US20130085073A1 (en) * 2011-09-29 2013-04-04 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nuclleic acids synthesis and sequencing
US20170218416A1 (en) * 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna

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US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US8653832B2 (en) 2010-07-06 2014-02-18 Sharp Kabushiki Kaisha Array element circuit and active matrix device
CN103502448B (zh) * 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
US8828336B2 (en) 2011-02-02 2014-09-09 Sharp Kabushiki Kaisha Active matrix device
US9492824B2 (en) 2013-01-16 2016-11-15 Sharp Kabushiki Kaisha Efficient dilution method, including washing method for immunoassay
US9169573B2 (en) 2013-01-23 2015-10-27 Sharp Kabushiki Kaisha AM-EWOD device and method of driving with variable voltage AC driving
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
EP3699283A1 (en) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
GB201502152D0 (en) 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
GB201503534D0 (en) 2015-03-03 2015-04-15 Nuclera Nucleics Ltd Novel method
GB201512372D0 (en) 2015-07-15 2015-08-19 Nuclera Nucleics Ltd Novel method
GB2559117B (en) * 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811811D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030194736A1 (en) * 2002-04-12 2003-10-16 Jurate Bitinaite Methods and compositions for DNA manipulation
CN1860227A (zh) * 2003-10-02 2006-11-08 巴斯福股份公司 用于序列饱和诱变(SeSaM)的方法
US20110269631A1 (en) * 2007-09-14 2011-11-03 Affymetrix, Inc. Amplification and Analysis of Selected Targets on Solid Supports
US20130085073A1 (en) * 2011-09-29 2013-04-04 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nuclleic acids synthesis and sequencing
US20170218416A1 (en) * 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116087504A (zh) * 2023-02-24 2023-05-09 上海碧云天生物技术有限公司 免疫磁珠保护液、其制备方法及应用
CN116087504B (zh) * 2023-02-24 2023-08-15 上海碧云天生物技术有限公司 免疫磁珠保护液、其制备方法及应用

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Publication number Publication date
AU2019304148A1 (en) 2021-01-14
US20210388432A1 (en) 2021-12-16
CA3104692A1 (en) 2020-01-23
EP3824100A1 (en) 2021-05-26
WO2020016604A1 (en) 2020-01-23
JP7393412B2 (ja) 2023-12-06
GB201811810D0 (en) 2018-09-05
JP2021529551A (ja) 2021-11-04
EP3824100B1 (en) 2026-04-29

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