CA3104692A1 - Polynucleotide synthesis method, kit and system - Google Patents
Polynucleotide synthesis method, kit and system Download PDFInfo
- Publication number
- CA3104692A1 CA3104692A1 CA3104692A CA3104692A CA3104692A1 CA 3104692 A1 CA3104692 A1 CA 3104692A1 CA 3104692 A CA3104692 A CA 3104692A CA 3104692 A CA3104692 A CA 3104692A CA 3104692 A1 CA3104692 A1 CA 3104692A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleotide
- strand
- polynucleotide
- synthesis
- cycle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1811810.9A GB201811810D0 (en) | 2018-07-19 | 2018-07-19 | Method |
| GB1811810.9 | 2018-07-19 | ||
| PCT/GB2019/052035 WO2020016604A1 (en) | 2018-07-19 | 2019-07-19 | Polynucleotide synthesis method, kit and system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3104692A1 true CA3104692A1 (en) | 2020-01-23 |
Family
ID=63364519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3104692A Pending CA3104692A1 (en) | 2018-07-19 | 2019-07-19 | Polynucleotide synthesis method, kit and system |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20210388432A1 (https=) |
| EP (1) | EP3824100B1 (https=) |
| JP (1) | JP7393412B2 (https=) |
| CN (1) | CN112424373A (https=) |
| AU (1) | AU2019304148A1 (https=) |
| CA (1) | CA3104692A1 (https=) |
| GB (1) | GB201811810D0 (https=) |
| WO (1) | WO2020016604A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2559117B (en) | 2017-01-19 | 2019-11-27 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method, kit and system |
| GB2574197B (en) | 2018-05-23 | 2022-01-05 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method and system. |
| GB201811813D0 (en) * | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| GB201811811D0 (en) | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| KR102281359B1 (ko) * | 2021-01-26 | 2021-07-22 | 씨제이제일제당 (주) | 신규한 단백질 변이체 및 이를 이용한 l-발린 생산 방법 |
| CN116087504B (zh) * | 2023-02-24 | 2023-08-15 | 上海碧云天生物技术有限公司 | 免疫磁珠保护液、其制备方法及应用 |
| GB202309649D0 (en) * | 2023-06-27 | 2023-08-09 | Oxford Nanopore Tech Plc | Method |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6479262B1 (en) * | 2000-05-16 | 2002-11-12 | Hercules, Incorporated | Solid phase enzymatic assembly of polynucleotides |
| JP4663988B2 (ja) * | 2002-04-12 | 2011-04-06 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | Ｄｎａ操作のための方法および組成物 |
| DK1670914T3 (da) * | 2003-10-02 | 2008-05-13 | Basf Se | Fremgangsmåde til sekvensmætnings-mutangenese (SeSaM) |
| US8716190B2 (en) * | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
| US8653832B2 (en) | 2010-07-06 | 2014-02-18 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
| CN103502448B (zh) * | 2010-11-12 | 2017-03-29 | Gen9股份有限公司 | 核酸合成的方法和设备 |
| US8828336B2 (en) | 2011-02-02 | 2014-09-09 | Sharp Kabushiki Kaisha | Active matrix device |
| US10378051B2 (en) * | 2011-09-29 | 2019-08-13 | Illumina Cambridge Limited | Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing |
| US9492824B2 (en) | 2013-01-16 | 2016-11-15 | Sharp Kabushiki Kaisha | Efficient dilution method, including washing method for immunoassay |
| US9169573B2 (en) | 2013-01-23 | 2015-10-27 | Sharp Kabushiki Kaisha | AM-EWOD device and method of driving with variable voltage AC driving |
| US9279149B2 (en) | 2013-04-02 | 2016-03-08 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| US9771613B2 (en) | 2013-04-02 | 2017-09-26 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acid |
| US20170218416A1 (en) * | 2014-05-16 | 2017-08-03 | The Regents Of The University Of California | Compositions and methods for single-molecule construction of dna |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
| EP3699283A1 (en) | 2014-10-20 | 2020-08-26 | Molecular Assemblies Inc. | Modified template-independent enzymes for polydeoxynucleotide systhesis |
| GB201502152D0 (en) | 2015-02-10 | 2015-03-25 | Nuclera Nucleics Ltd | Novel use |
| GB201503534D0 (en) | 2015-03-03 | 2015-04-15 | Nuclera Nucleics Ltd | Novel method |
| GB201512372D0 (en) | 2015-07-15 | 2015-08-19 | Nuclera Nucleics Ltd | Novel method |
| GB2559117B (en) * | 2017-01-19 | 2019-11-27 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method, kit and system |
| GB2574197B (en) * | 2018-05-23 | 2022-01-05 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method and system. |
| GB201811811D0 (en) * | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| GB201811813D0 (en) * | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
-
2018
- 2018-07-19 GB GBGB1811810.9A patent/GB201811810D0/en not_active Ceased
-
2019
- 2019-07-19 AU AU2019304148A patent/AU2019304148A1/en not_active Abandoned
- 2019-07-19 CA CA3104692A patent/CA3104692A1/en active Pending
- 2019-07-19 CN CN201980048279.1A patent/CN112424373A/zh active Pending
- 2019-07-19 EP EP19748892.7A patent/EP3824100B1/en active Active
- 2019-07-19 US US17/260,611 patent/US20210388432A1/en active Pending
- 2019-07-19 JP JP2021502451A patent/JP7393412B2/ja active Active
- 2019-07-19 WO PCT/GB2019/052035 patent/WO2020016604A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU2019304148A1 (en) | 2021-01-14 |
| CN112424373A (zh) | 2021-02-26 |
| US20210388432A1 (en) | 2021-12-16 |
| EP3824100A1 (en) | 2021-05-26 |
| WO2020016604A1 (en) | 2020-01-23 |
| JP7393412B2 (ja) | 2023-12-06 |
| GB201811810D0 (en) | 2018-09-05 |
| JP2021529551A (ja) | 2021-11-04 |
| EP3824100B1 (en) | 2026-04-29 |
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| EP3797170B1 (en) | Polynucleotide synthesis method, system and kit | |
| US20240318219A1 (en) | Methods and reagents for synthesising polynucleotide molecules | |
| US20250084468A1 (en) | Polynucleotide synthesis method, kit and system | |
| US20250263767A1 (en) | Polynucleotide synthesis method, kit and system | |
| EP3824100B1 (en) | Polynucleotide synthesis method | |
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