CN112386562A - Plant fermentation method and composition thereof - Google Patents
Plant fermentation method and composition thereof Download PDFInfo
- Publication number
- CN112386562A CN112386562A CN202011214389.1A CN202011214389A CN112386562A CN 112386562 A CN112386562 A CN 112386562A CN 202011214389 A CN202011214389 A CN 202011214389A CN 112386562 A CN112386562 A CN 112386562A
- Authority
- CN
- China
- Prior art keywords
- sunflower
- micrococcus
- fermentation
- plant
- strains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a plant fermentation method and a composition thereof, and the scheme comprises the following steps of S1: activating microsphere strains; s2: screening the microsphere strains in a UVB radiation environment to screen out the viable microsphere strains; s3: inoculating the micrococcus in the stable phase which survives in the S2 and carrying out expanded culture; s4: extracting sunflower to obtain sunflower extract; s5: adding the sunflower extract into the microspherical bacteria culture solution in S3 and performing fermentation culture; s6: the culture solution obtained in the step S5 is subjected to centrifugal filtration to remove impurities to obtain a sunflower-micrococcus fermentation product, and the sunflower-micrococcus fermentation product has high-efficiency antioxidant activity and is more suitable for skin conditioning.
Description
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to a plant fermentation method and a composition thereof.
Background
With the growing concern of consumers on skin beauty and health in recent years and the continuous harsh, stressful and daily rise of environment, consumers increasingly know the importance of body and skin health. Microbial fermentation refers to a process of converting raw materials into products which are easily absorbed and utilized by the human body through a specific metabolic pathway by utilizing the decomposition action of microorganisms under appropriate conditions. The separation of active ingredients is promoted through the growth and metabolic activities of microorganisms, so that a new effect is generated, the medicinal value is enhanced, and the absorption and utilization of the active ingredients by an organism are promoted.
The plant and microorganism co-fermentation is to extract juice or extract plant raw materials and then add probiotics for fermentation, so that a large amount of effective active ingredients can be generated. With the continuous progress of research, a great number of scientific experiments report the application of the co-fermentation of plants and microorganisms in the directions of improving the medicinal effect, food and beverage, health care products, purifying pollution and the like.
However, the commonly used co-fermentation microorganisms with plants mainly include bifidobacteria, lactobacilli, yeasts, partial fungi, endophytic fungi and the like, and have poor skin conditioning effect, so that the increasing demands of consumers cannot be met.
Disclosure of Invention
The present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a method for fermenting a plant and a composition thereof.
In order to realize the purpose of the invention, the invention adopts the following technical scheme: a plant fermentation method comprises the following steps:
s1: activating microsphere strains;
s2: screening the microsphere strains in a UVB radiation environment to screen out the viable microsphere strains;
s3: inoculating the micrococcus in the stable phase which survives in the S2 and carrying out expanded culture;
s4: extracting sunflower to obtain sunflower extract;
s5: adding the sunflower extract into the microspherical bacteria culture solution in S3 and performing fermentation culture;
s6: and (4) centrifuging and filtering the culture solution obtained in the step (S5) to remove impurities to obtain a sunflower-micrococcus fermentation product.
The working principle and the beneficial effects are as follows: the micrococcus is directionally improved and optimized to enable the micrococcus to have the capability of resisting UVB radiation, so that a product fermented by the micrococcus has good antioxidant activity, wherein the sunflower is also a plant which is acknowledged to be capable of resisting ultraviolet growth in nature, and researches prove that a sunflower extracting solution has high-efficiency antioxidant activity, so that the sunflower-micrococcus fermentation product extracted by combining the sunflower and the micrococcus through fermentation has obvious antioxidant activity, comprises a large amount of active ingredients such as polyphenols, polypeptides, polysaccharides and reduced glutathione and the like which can promote skin moisturizing, antioxidant, anti-inflammatory, whitening and the like, and has excellent antioxidant, repairing, anti-inflammatory and whitening effects on human skin; compared with the prior art, micrococcus are aerobically grown gram-positive cocci which are ubiquitous in the natural world, such as air, soil, water, plants, food and the like, and also inhabit and grow on the skin of mammals. Micrococcus are generally considered to be harmless saprophytic bacteria, colonize human skin and respiratory mucosal surfaces, and are detectable on exposed faces, arms and legs, so that the product fermented by the combination of micrococcus and sunflower extract is also less harmful to humans.
Further, in step S1, microspherical strains are inoculated in a slant culture medium and cultured for 36h at 30 ℃ to activate the microspherical strains.
Further, in step S2, the well-grown strains are inoculated in a liquid culture medium, centrifugal shaking culture is carried out for 48 hours at 150r/min in a UVB radiation environment, the temperature is 30 ℃, and microspherical strains capable of growing under the UVB radiation condition are screened.
Further, the micrococcus screened in step S3 to be in the stationary phase is placed in a liquid medium and cultured by a shaker until the growth phase reaches the logarithmic phase, wherein the rotation speed of the shaker is 150r/min and the temperature is 30 ℃.
Further, in step S4, the whole sunflower is pulverized and put in an ultrasonic tank with 60% ethanol, and the pulverized sunflower is put in an ultrasonic tank and extracted for 4 hours at 75 ℃, and is subjected to rotary evaporation at 50 ℃ by a rotary evaporator to obtain the sunflower extract.
A plant fermentation composition comprises a sunflower-micrococcus fermentation product obtained by the above plant fermentation method, and also comprises one or more of a solubilizer, a preservative, an antioxidant, a pH regulator, a penetration enhancer, a liposome, a humectant, a thickener, a chelating agent, a skin feel regulator, a surfactant, an emulsifier, an essence and a pigment.
The composition is used for skin care, has the effects of whitening skin, removing spots, promoting skin wound healing, resisting inflammation and activating a peroxidase-activated receptor.
Further, the composition is in the form of one of cream, lotion, water agent and film agent.
Further, the sunflower-micrococcus fermentation product accounts for 0.001-100% of the total weight of the composition.
Further, the composition is a skin care essence, and the components of the skin care essence at least comprise: water, glyceryl polyether-26, allantoin, sodium polyglutamate, acrylate/C10-30 alkanol acrylate cross-linked polymer, methylparaben, disodium EDTA, dipropylene glycol, polyethylene glycol, butanediol, sodium hyaluronate, xanthan gum, bis-PEG-18 methyl ether dimethylsilane, octyl glycol, phenoxyethanol, sunflower-micrococcus fermentation product, PEG-40 hydrogenated castor oil and essence.
Furthermore, the content of the sunflower-micrococcus fermentation product is 0.5-20%.
Drawings
FIG. 1 is a graph showing the growth of microspheroidal strains according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments that can be derived by one of ordinary skill in the art from the embodiments given herein are intended to be within the scope of the present invention.
It will be understood by those skilled in the art that in the present disclosure, the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for ease of description and simplicity of description, and do not indicate or imply that the referenced devices or components must be in a particular orientation, constructed and operated in a particular orientation, and thus the above terms are not to be construed as limiting the present invention.
The plant fermentation method comprises the following steps:
s1: inoculating a strain purchased from China center for industrial microorganism strain preservation management in a slant culture medium, and culturing at 30 ℃ for 36h to activate microspherical strains; inoculating the well-grown strains in a liquid culture medium, carrying out 150r/min in a UVB radiation environment, carrying out shake culture at 30 ℃ for 48h, screening microspherical strains capable of growing under the UVB radiation condition, calculating a growth curve as shown in figure 1, and facilitating later-stage further experiment culture of micrococcus with better UVB radiation resistance, wherein the preservation number of the microspherical strains is CICC 23810; the micrococcus screened out at this time has extremely strong radiation resistance, which means that the micrococcus has extremely strong oxidation resistance.
S2: screening S1 to obtain micrococcus in stationary phase, placing the micrococcus in liquid culture medium at 150r/min, and performing shaking table culture at 30 ℃ until logarithmic phase;
s3: pulverizing whole sunflower, adding 60% ethanol, ultrasonic extracting at 75 deg.C for 4 hr in ultrasonic tank, and rotary evaporating at 50 deg.C to obtain brown viscous substance to obtain sunflower extract;
s4: adding the sunflower extract obtained in S3 into the microspherical fungus culture solution in S2, fermenting and culturing, and culturing at 150r/min in a shaking table at 30 ℃ for about 48 h;
s5: and (2) centrifuging the fermentation culture solution obtained in the step (S4), filtering by using a plate frame, intercepting thalli on a filtering membrane (0.22 mu m) to obtain fermentation supernatant, adding activated carbon into the fermentation supernatant to adsorb impurities in the fermentation supernatant, removing peculiar smell, centrifuging by using a plurality of layers of absorbent gauze to remove carbon residue, and filtering the purified fermentation broth through the filtering membrane of 0.22 mu m to obtain the sunflower-micrococcus fermentation product.
The materials and equipment used in the above steps are: peptone, beef extract, sodium chloride, agar, pure water, whole sunflower, an ultrasonic instrument, a rotary evaporator, an autoclave, a biochemical incubator, a PH meter and the like.
Wherein the preparation and components of the culture solution are as follows:
slant culture medium, peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, agar 15.0g, and pure water 1000mL, with pH 7.2, 121 deg.C, and sterilizing for 20 min;
liquid culture medium, 10.0g of peptone, 3.0g of beef extract, 5.0g of sodium chloride and 1000mL of pure water, wherein the pH value is 7.2, the temperature is 121 ℃, and the sterilization is carried out for 20 min.
Because the skin conditioning effect of the single micrococcus fermentation product and the sunflower extract applied to the skin care product is poor, the sunflower-micrococcus fermentation product prepared by the method can degrade organic matters which cannot be directly utilized by a human body in the sunflower extract through the fermentation of the micrococcus, so that the organic matters can be better absorbed by the skin of the human body, and the sunflower-micrococcus fermentation product prepared by the method has stronger effect in the skin care product applied to the skin care product.
S6: the content of polypeptide, polyphenol and polysaccharide in the sunflower-micrococcus fermentation product is detected by preparing materials and equipment such as casein, sodium hydroxide, anhydrous copper sulfate, gallic acid, a forskolin phenol solution, sodium carbonate, glucose, concentrated sulfuric acid, phenol and a reduced glutathione detection kit, pure water, an ultraviolet spectrophotometer, a colorimetric tube, a 10mm cuvette, a water bath kettle and the like.
The structures detected are as follows:
the polypeptide is one of peptide or peptide, and is a compound with a molecular structure between amino acid and protein. Amino acids are the smallest constitutional unit of proteins. An amino acid molecule is called an amino acid. Two amino acid molecules are dehydrated, condensed, connected and combined together to form the second peptide, and by analogy, the second peptide contains four amino acids and is called the fourth peptide; contains nine amino acids, named as Jiuzhanyun; peptides of less than 10 amino acids are generally called oligopeptides, peptides with a larger number of amino acids are called polypeptides, and peptides with more than 50 amino acids are called proteins. Because the varieties of the contained amino acids are different, the di-peptide and the tri-peptide also have different varieties, so that various peptides with different names and different functions appear. In short, amino acid constitutes peptide, peptide constitutes protein, but protein can not be absorbed and utilized by human body directly, but single amino acid and peptide chain separated from protein can be absorbed by human body, so that the polypeptide content in the sunflower-micrococcus fermentation product is higher, and the sunflower-micrococcus fermentation product has better absorption effect by human body.
Wherein the polyphenols are compounds found in vegetable foods with potential health promoting effects. It is found in some common plant foods, such as cocoa beans, popcorn, tea, soy beans, red wine, vegetables and fruits. The antioxidant function of polyphenol can prevent the chronic diseases; the plant polyphenol has the common properties of astringing the body, removing the body, resisting ultraviolet radiation, inhibiting the activities of the tyrosine and the peroxidase, and keeping various physiological activities, so that the plant polyphenol has various unique effects in the preparation of anti-aging and whitening cosmetics.
Wherein the chemical name of the reduced glutathione is N- (N-L-gamma-glutamyl-L-cysteinyl) glycine, the molecular formula is C10H17N3O6S, the molecular weight is 307.33, and the reduced glutathione is soluble in water, diluted alcohol, liquid ammonia and methyl formamide, but not soluble in alcohol, ether and acetone. It can be used for treating facial pigmentation, sweat stain and pigmentation caused by various reasons, and also has good antioxidant effect.
Therefore, the sunflower-micrococcus fermentation product prepared by the method has good functions of resisting oxidation, resisting aging, whitening, removing acnes, diminishing inflammation and repairing human skin, and can be better absorbed by a human body, which cannot be realized by a single sunflower extract.
A plant fermentation composition comprises the sunflower-micrococcus fermentation product obtained by the plant fermentation method, and also comprises auxiliary materials acceptable in the field of cosmetics, such as one or more of a solubilizer, a preservative, an antioxidant, a pH regulator, a penetration enhancer, a liposome, a humectant, a thickener, a chelating agent, a skin feel regulator, a surfactant, an emulsifier, an essence and a pigment.
Preferably, the composition may be formulated into, for example, a solution, a suspension, an emulsion, a gel, a cream, a surfactant-containing cleanser, a powder foundation, an emulsion foundation, a spray, etc., but is not limited thereto.
More preferably, it can be made into basic cosmetics such as astringent, lotion, body lotion, nourishing cream, moisturizing cream, hand cream, essence, eye cream, cleansing foam, cleansing lotion, pack, gel, etc., and color cosmetics such as pre-makeup lotion, foundation, etc.
Preferably, the sunflower-micrococcus fermentation product is present in an amount of 0.001% to 100%, preferably 0.01% to 50%, more preferably 0.5% to 20% by weight of the total composition, within the range of the extent that it can be absorbed by the human body, without waste, and at a cost effectively reduced.
When the dosage form of the present invention is a solution or emulsion, a solvent, a solubilizer, or an emulsifier, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol, glycerol fatty acid ester, polyethylene glycol, or fatty acid ester of sorbitan, may be used as the carrier.
When the dosage form of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohols, polyoxyethylene sorbitol esters and polyoxyethylene sorbitol esters, microcrystalline cellulose, meta-aluminate, bentonite, agar or tragacanth may be used as carriers.
When the formulation of the present invention is surfactant-containing, fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazoline derivative, methyl taurine, sarcosinate, fatty acid amide ether sulfate, alkylamide betaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerin fatty acid ester, or the like can be used as a carrier.
The thickener contained in the composition of the present invention may be methylcellulose, carboxymethylcellulose, carboxymethyl hydroxyguanine, hydroxymethylcellulose, hydroxyethylcellulose, carbopol, polyquaternary ammonium salt, cetostearyl alcohol, stearic acid, carrageenan, or the like.
The composition of the present invention may comprise a substance selected from the group consisting of vitamins, polypeptides, polysaccharides, sphingolipids and seaweed extracts.
In addition, oils, fats, moisturizers, emollients, surfactants, organic or inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, other plant extracts, pH adjusters, alcohols, colorants, fragrances, purified water, and the like may be further contained.
However, the ingredients contained in the composition may not be limited thereto. Moreover, the content of the components may be determined within a range not affecting the object and effect of the present invention.
A skin care essence prepared from the composition is prepared by the following steps:
adding phase A in the formula into an emulsifying pot, heating to 85 ℃, and stirring for 20min under the condition of heat preservation; adding the premixed B, C phase into an emulsifying pot, and uniformly stirring; cooling to 55 deg.C, adding phase D, and stirring; cooling to 45 deg.C, adding E, F phase, and stirring; cooling to 37 deg.C, discharging, weighing and packaging.
The raw material of A, B, C, D, E, F phase is as follows:
compared with the existing product without sunflower-micrococcus fermentation product, the skin care essence prepared by the method has better effects of resisting oxidation, whitening and removing acnes.
Application example: in order to show the care effect of the skin care essence, cases of skin inflammation of different ages and different sexes are specially selected, the skin care essence is used for treatment, and one treatment course is 60 days.
It is stated that most patients have other symptoms, such as acne, pox, etc., to varying degrees.
The effects are as follows:
in the process of using the skin care essence, acne, pox and the like of most patients are relieved and healed to different degrees, after a treatment course, the skin condition of more than 92.5 percent of the patients is obviously improved and even recovered to a healthy level compared with that before the patients are used, and the skin is smoother, fair, slows down aging and is more elastic.
The present invention is not described in detail in the prior art, and therefore, the present invention is not described in detail.
It is understood that the terms "a" and "an" should be interpreted as meaning that a number of one element or element is one in one embodiment, while a number of other elements is one in another embodiment, and the terms "a" and "an" should not be interpreted as limiting the number.
Although terms are used more often herein, the possibility of using other terms is not excluded. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed as being without limitation to any additional limitations that may be imposed by the spirit of the present invention.
The present invention is not limited to the above-mentioned preferred embodiments, and any other products in various forms can be obtained by anyone in the light of the present invention, but any changes in the shape or structure thereof, which have the same or similar technical solutions as those of the present application, fall within the protection scope of the present invention.
Claims (10)
1. A plant fermentation process comprising the steps of:
s1: activating microsphere strains;
s2: screening the microsphere strains in a UVB radiation environment to screen out the viable microsphere strains;
s3: inoculating the micrococcus in the stable phase which survives in the S2 and carrying out expanded culture;
s4: extracting sunflower to obtain sunflower extract;
s5: adding the sunflower extract into the microspherical bacteria culture solution in S3 and performing fermentation culture;
s6: and (4) decoloring and deodorizing the culture solution obtained in the step (S5), and filtering and removing impurities to obtain a sunflower-micrococcus fermentation product.
2. The plant fermentation method of claim 1, wherein in step S1, microspherical strain is inoculated in slant culture medium and cultured at 30 ℃ for 36h to activate microspherical strain.
3. The plant fermentation method of claim 1, wherein the well-grown strain is inoculated into a liquid culture medium in step S2, and the strain is subjected to centrifugal shaking culture at 150r/min in UVB radiation environment for 48h at 30 ℃, and microspherical strains capable of growing under UVB radiation conditions are screened.
4. The plant fermentation method of claim 1, wherein the micrococcus which is screened in step S3 and is in stationary phase is placed in a liquid medium and cultured by a shaker until logarithmic growth phase, the rotation speed of the shaker is 150r/min, and the temperature is 30 ℃.
5. The plant fermentation method of claim 1, wherein in step S4, the whole sunflower is pulverized and put in an ultrasonic tank with 60% ethanol, the pulverized sunflower is put in an ultrasonic tank and extracted for 4 hours at 75 ℃, and the sunflower extract is obtained by rotary evaporation at 50 ℃ through a rotary evaporator.
6. A plant fermentation composition comprising a sunflower micrococcus fermentation product obtained by a plant fermentation process according to any one of claims 1-5, and further comprising one or more of a solubilizing agent, a preservative, an antioxidant, a pH adjusting agent, a penetration enhancer, a liposome, a humectant, a thickener, a chelating agent, a skin feel modifier, a surfactant, an emulsifier, a fragrance, and a pigment.
7. The plant fermented composition according to claim 6, wherein the composition is in the form of one of cream, lotion, water agent and film agent.
8. A plant fermented composition according to claim 6, wherein said sunflower-Micrococcus fermentans fermentation product is present in an amount of 0.001-100% by weight of the total composition.
9. The plant fermented composition according to any one of claims 6 to 8, wherein the composition is a skin care essence, and the skin care essence comprises at least the following components: water, glyceryl polyether-26, allantoin, sodium polyglutamate, acrylate/C10-30 alkanol acrylate cross-linked polymer, methylparaben, disodium EDTA, dipropylene glycol, polyethylene glycol, butanediol, sodium hyaluronate, xanthan gum, bis-PEG-18 methyl ether dimethylsilane, octyl glycol, phenoxyethanol, sunflower-micrococcus fermentation product, PEG-40 hydrogenated castor oil and essence.
10. The plant fermentation composition of claim 9, wherein the sunflower-micrococcus fermentation product is present in an amount of 0.5% to 20%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011214389.1A CN112386562B (en) | 2020-11-04 | 2020-11-04 | Application of plant fermentation method in skin care product and composition thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011214389.1A CN112386562B (en) | 2020-11-04 | 2020-11-04 | Application of plant fermentation method in skin care product and composition thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112386562A true CN112386562A (en) | 2021-02-23 |
| CN112386562B CN112386562B (en) | 2022-06-10 |
Family
ID=74598170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011214389.1A Active CN112386562B (en) | 2020-11-04 | 2020-11-04 | Application of plant fermentation method in skin care product and composition thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112386562B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115414313A (en) * | 2022-10-14 | 2022-12-02 | 佛山天韵化妆品科技有限公司 | Anti-aging composition containing micrococcus lysate and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100260695A1 (en) * | 2009-04-09 | 2010-10-14 | Mary Kay Inc. | Combination of plant extracts to improve skin tone |
| CN101914594A (en) * | 2010-08-13 | 2010-12-15 | 武汉嘉发胶原蛋白研究所 | Biological fermentation extracting method for hyaluronic acid |
| JP2018008903A (en) * | 2016-07-14 | 2018-01-18 | 共栄化学工業株式会社 | Weight-reducing composition |
| WO2018073278A1 (en) * | 2016-10-18 | 2018-04-26 | Universite Paris Descartes | Cosmetic composition for preventing and/or treating body odor |
| JP2018193341A (en) * | 2017-05-19 | 2018-12-06 | 共栄化学工業株式会社 | Plant fermentation product |
| CN109172448A (en) * | 2018-10-12 | 2019-01-11 | 广州市雅彩盛生物科技有限公司 | A kind of skin care item and preparation method thereof with anti-blue light action |
| CN111235058A (en) * | 2020-02-20 | 2020-06-05 | 江苏微康生物科技有限公司 | Bifidobacterium breve with effects of repairing ultraviolet injury, relieving inflammation and preventing skin photoaging, and its preparation method and application |
-
2020
- 2020-11-04 CN CN202011214389.1A patent/CN112386562B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100260695A1 (en) * | 2009-04-09 | 2010-10-14 | Mary Kay Inc. | Combination of plant extracts to improve skin tone |
| CN101914594A (en) * | 2010-08-13 | 2010-12-15 | 武汉嘉发胶原蛋白研究所 | Biological fermentation extracting method for hyaluronic acid |
| JP2018008903A (en) * | 2016-07-14 | 2018-01-18 | 共栄化学工業株式会社 | Weight-reducing composition |
| WO2018073278A1 (en) * | 2016-10-18 | 2018-04-26 | Universite Paris Descartes | Cosmetic composition for preventing and/or treating body odor |
| JP2018193341A (en) * | 2017-05-19 | 2018-12-06 | 共栄化学工業株式会社 | Plant fermentation product |
| CN109172448A (en) * | 2018-10-12 | 2019-01-11 | 广州市雅彩盛生物科技有限公司 | A kind of skin care item and preparation method thereof with anti-blue light action |
| CN111235058A (en) * | 2020-02-20 | 2020-06-05 | 江苏微康生物科技有限公司 | Bifidobacterium breve with effects of repairing ultraviolet injury, relieving inflammation and preventing skin photoaging, and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 疏翠: "藤黄微球菌过氧化氢酶发酵条件优化、诱变育种及其基因的克隆、表达与纯化", 《中国优秀硕士学位论文全文数据库（电子期刊）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115414313A (en) * | 2022-10-14 | 2022-12-02 | 佛山天韵化妆品科技有限公司 | Anti-aging composition containing micrococcus lysate and preparation method thereof |
| CN115414313B (en) * | 2022-10-14 | 2023-11-14 | 佛山天韵化妆品科技有限公司 | Anti-aging composition containing micrococcus lysate and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112386562B (en) | 2022-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101464745B1 (en) | Compositions comprising fermentative extract of Orchidaceae for improving functions of skin | |
| CN110734932B (en) | Fermented birch juice and its production method | |
| KR20110096102A (en) | Antimicrobial composition with extracts of natural materials, naturotics and manufacturing method thereof | |
| KR101615909B1 (en) | Fermentation oil cosmetic composition having anti-oxidant effect. | |
| KR20110108686A (en) | Antioxidant containing extracts of natural materials and composition of feed, food or cosmetic | |
| KR101303295B1 (en) | The cosmetic composition for promoting cell growth and recovery of distrupted skin containing the extracts of fermented Mistletoe, fermented Imperata cylindrica and fermented soybean | |
| KR101677362B1 (en) | Cosmetic composition comprising fermented Phellinus linteus extract | |
| KR101229045B1 (en) | Extracting method of nelumbo nucifera flower, leaf, stem, root, seed and cosmetic composition for anti-wrinkle containing extracts thereby | |
| KR101532003B1 (en) | Cosmetics comprising fermented materials for skin antioxidant | |
| CN112386562B (en) | Application of plant fermentation method in skin care product and composition thereof | |
| KR101531996B1 (en) | Cosmetics comprising fermented materials for skin whitening | |
| KR102319701B1 (en) | Cosmetic composition comprising mixture of fermented extract of Cirsium japonicum and Moringa oleifera | |
| KR20170052811A (en) | yeast fermented solutions of green tea extract and manufacturing method thereof and cosmetic composition using the same | |
| JP3435181B2 (en) | External preparation for melanin production suppression | |
| KR101532001B1 (en) | Cosmetics comprising fermented materials for preventing or improving skin wrinkle | |
| KR102560734B1 (en) | Method for preparing Centella asiatica Extracts by three-step fermentation and Cosmetic composition comprising thereof | |
| KR20130102712A (en) | Anti-heat aging effect of fermented limonia acidissima extract and its cosmetic usage to improve skin problems | |
| KR20190049067A (en) | A cosmetic composition comprising extract of coffee silver skin and coffee grounds | |
| KR100534074B1 (en) | Cosmetic composition containing grain extract and fermented grain phellinus linteus extract, and cosmetics containing the same | |
| JP2024500509A (en) | Novel Saccharomyces cerevisiae strains and their uses | |
| KR20130143241A (en) | Composite natural antiseptics from forsythia suspensa vahl, leonurus sibiricus l., crataegi fructus and akebia quinata decaisne, cosmetic compositions containing them | |
| KR20110122448A (en) | Cosmetic composition comprising the extract of filipendula formosa as active ingredient | |
| JP2021004214A (en) | Combinational fermented product | |
| KR20170041399A (en) | Mask pack composition containing velvet-extract | |
| KR100829831B1 (en) | A skin whitening compositions comprising natural plant extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Application and composition of a plant fermentation method in skincare products Effective date of registration: 20230612 Granted publication date: 20220610 Pledgee: Bank of Jiangsu Limited by Share Ltd. Hangzhou branch Pledgor: Zhejiang Moda Biotechnology Co.,Ltd. Registration number: Y2023330001178 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |