CN112322480A

CN112322480A - Continuous automatic cultivation system for aerobic bacteria

Info

Publication number: CN112322480A
Application number: CN202011584853.6A
Authority: CN
Inventors: 罗千松
Original assignee: Songta Intellectual Property Operation Wuhan Co ltd
Current assignee: Songta Intellectual Property Operation Wuhan Co ltd
Priority date: 2020-12-29
Filing date: 2020-12-29
Publication date: 2021-02-05

Abstract

The invention provides a continuous automatic culture system for aerobic bacteria, which comprises a strain culture area; the strain cultivation area is internally provided with a plurality of shaking platforms which are mutually connected into a production line, and the strain cultivation boxes are driven to walk along the production line of the shaking platforms through a plurality of push-pull conveying devices so as to finish strain cultivation. The structure of the strain culture box is as follows: the two sides of the box body are provided with side wall structures which can be opened and closed, a culture blanket is arranged in the box body, a culture medium is arranged on the inner surface of the culture blanket, the culture blanket forms a water storage structure in the box body, and when the side wall of the box body is opened, the two sides of the culture blanket slide down to enable the bacteria liquid to flow out. Through adopting the scheme that the push-pull conveying device drives the plurality of strain culture boxes to move, the continuous automatic production of natto can be realized, and the time for moving the strain culture boxes and the bean fermentation boxes in the strain culture area and the solid fermentation area is the time required by the whole production, so that the continuous automatic production of products is realized.

Description

Continuous automatic cultivation system for aerobic bacteria

Technical Field

The invention relates to the technical field of automatic production, in particular to an automatic aerobic bacterium cultivation system.

Background

The existing production process of aerobic bacteria, such as bacillus natto and natto finished products thereof, mostly adopts a mode of artificial auxiliary partial mechanical conveying equipment production, such as the preparation method described in CN103829166A and CN 106722201A. Bacillus natto belongs to aerobic bacteria, needs a large amount of oxygen to participate in the production process, and the tank cultivation method commonly used for microbial production in the prior art is not suitable for the cultivation of the bacillus natto. And the artificial participation causes that the mixed bacteria in the production process are difficult to eliminate, and the quality of the final finished product is influenced. In the preparation method in the prior art, the cultivation of the strains consumes the largest amount of labor in the production process, and the culture medium is easily mixed into the bacterial liquid by adopting the existing industrial large-scale production method, so that the quality of the final finished product is influenced. Through search, no better solution in the prior art is found.

Disclosure of Invention

The invention aims to provide a continuous automatic culture system for aerobic bacteria, which can realize continuous automatic production of aerobic bacteria, reduce the influence of artificial participation on product quality, be suitable for large-scale industrial production and be convenient for realizing an intelligent production mode without human participation.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: an aerobic bacteria continuous automatic cultivation system comprises a strain cultivation area;

the strain cultivation area is internally provided with a plurality of shaking platforms which are mutually connected into a production line, and the strain cultivation boxes are driven to walk along the production line of the shaking platforms through a plurality of push-pull conveying devices so as to finish strain cultivation.

In a preferred scheme, the structure of the strain culture box is as follows: the two sides of the box body are provided with side wall structures which can be opened and closed, a culture blanket is arranged in the box body, a culture medium is arranged on the inner surface of the culture blanket, the culture blanket forms a water storage structure in the box body, and when the side wall of the box body is opened, the two sides of the culture blanket slide down to enable the bacteria liquid to flow out.

In a preferred scheme, the structure of the strain culture box is as follows: the lateral wall of box body is equipped with the spout, and box body slip lateral wall slidable mounting is in the spout, and box body slip lateral wall is connected with the box body through the extension spring to make box body slip lateral wall be in the block state.

The bottom of the box body is provided with an extended foot part, the height of the extended foot part is greater than or equal to that of the sliding side wall of the box body, the bottom of the sliding side wall of the box body is provided with a deflector rod, and the deflector rod is used for driving the sliding side wall of the box body to slide downwards to be in an opening state;

the culture blanket is arranged in the box body, the culture medium is arranged on the inner surface of the culture blanket, the culture blanket forms a water storage structure in the box body, and when the side wall of the box body is opened, the two sides of the culture blanket slide down to enable the bacteria liquid to flow out.

In the preferred scheme, a bacterial liquid collecting platform is also arranged in the strain cultivation area;

one end of the bacteria liquid collecting platform is provided with a push-pull conveying device for driving the strain culture box to walk;

the head end of the bacteria liquid collecting platform is connected with the tail end of the shaking platform production line;

an opening guide rail is arranged on at least one side of the bacteria liquid collecting platform, the bottom of the opening guide rail is in contact with a deflector rod, one end of the opening guide rail, which is close to the head end of the bacteria liquid collecting platform, is higher, the other end of the opening guide rail is lower, and the opening guide rail is used for pressing down the deflector rod to enable the sliding side wall of the box body to slide downwards to be;

and a bacterium liquid collecting tank is arranged below at least one side of the bacterium liquid collecting platform and is used for collecting bacterium liquid.

In a preferred embodiment, the structure of the culture blanket is: the outer side or the inner side of the fabric is provided with a waterproof film;

the fabric is made of cotton cloth or chemical fiber, and the waterproof film is made of plastic film or agar.

In a preferable scheme, the push-pull conveying device is structurally characterized in that a push plate or a pull plate is arranged at the end of a piston rod of the air cylinder and used for pushing and pulling one position of the strain culture box, and the one position refers to one length or width distance of the strain culture box.

In the preferred scheme, a plurality of shaking platforms form an array structure, the tops of the shaking platforms are flush, and one end of each row of the shaking platforms is provided with a push-pull conveying device for pushing, so that a plurality of strain culture boxes can be pushed to walk along the current row; the other end of each row of the plurality of shaking platforms is provided with a push-pull conveying device for side pulling or side pushing, and the push-pull conveying device is used for enabling the strain culture boxes to move.

In a preferred scheme, the structure of the shaking platform is as follows: the top of the support is provided with a plurality of elastic elements, the top of the elastic elements is fixedly connected with the flat plate, and the support is provided with a cam driving mechanism or a crank rocker mechanism to drive the flat plate to swing.

In the preferred scheme, a temperature control device is arranged in the strain cultivation area;

the strain cultivation area is also connected with an air supply device, an air sterilization and filtration device is arranged at the inlet of the air supply device and used for sending filtered air into the strain cultivation area and the solid fermentation area, and the strain cultivation area and the solid fermentation area are in positive pressure.

In the preferred scheme, a culture medium disinfection room is arranged at an inlet of the strain culture area, and an array type inoculation device is arranged in the culture medium disinfection room and is used for inoculating the strain culture box;

a push-pull conveying device is arranged in the culture medium disinfection room and is used for driving the strain culture box to walk or enter the strain culture area;

a flashboard is arranged between the culture medium disinfection room and the strain cultivation area.

The invention provides a continuous automatic aerobic bacteria cultivation system, which can realize the continuous automatic production of natto by adopting a scheme that a push-pull conveying device drives a plurality of strain cultivation boxes to move, and the time for moving the strain cultivation boxes and the bean fermentation boxes in a strain cultivation area and a solid fermentation area is the time required by the whole production, thereby realizing the continuous automatic production of products. The strain culture box that sets up combines array inoculation device can adapt to the large-scale automatic inoculation and the cultivation of bacterial, but also can be convenient collect the fungus liquid with the mode that flows automatically, be difficult to mix the culture medium in the fungus liquid, also need not artificial participation, improve cultivation efficiency and finished product quality by a wide margin. The whole production process of the invention is completed in a closed positive pressure space, thereby avoiding the interference of mixed bacteria to the maximum extent and obtaining high-purity bacteria liquid. The automatic production system of the invention can be used for producing other aerobic bacteria related products besides being used for producing bacillus natto related products.

Drawings

The invention is further illustrated by the following examples in conjunction with the accompanying drawings:

FIG. 1 is a schematic top view of the structure of the present invention.

Fig. 2 is a schematic top view of the overall structure of the present invention.

Fig. 3 is a schematic structural view of the rocking platform of the present invention.

Fig. 4 is another preferred structure of the rocking platform of the present invention.

FIG. 5 is a side view of the bacteria liquid collection platform of the present invention.

FIG. 6 is a schematic cross-sectional view of a seed culture cassette according to the present invention.

FIG. 7 is a schematic cross-sectional view of the bacterial culture cassette of the present invention when collecting bacterial liquid.

Fig. 8 is a top view of the material transfer cart of the present invention.

In the figure: a strain cultivation area 1, a solid fermentation area 2, a push-pull conveying device 3, a shaking platform 4, a flat plate 41, an elastic element 42, a support 43, a cam driving mechanism 44, a crank rocker mechanism 45, a strain liquid collecting tank 5, a strain liquid box 6, a strain liquid collecting platform 7, a strain culture box 8, a box body 81, a culture blanket 82, a deflector rod 83, a chute 84, a tension spring 85, a box body sliding side wall 86, a strain liquid 87, a culture medium 88, a lifting device 9, a conveying device 10, a temperature control device 11, an air sterilization and filtration device 12, an air supply device 13, a bean sterilization room 14, a bean fermentation box 15, a bean fermentation box conveying device 16, a culture medium conveying device 17, a crushing device 18, an array inoculation device 19, a strain temporary storage kettle 20, a culture medium sterilization room 21, a material transfer trolley 22, a rotating roller 221, a driving chain 222, a moving vehicle body 223, a push plate 224, an after-ripening device 23, a fermentation platform 25, a bacteria liquid inoculation box 26, an opening guide rail 27 and a flashboard 28.

Detailed Description

Example 1:

as shown in FIG. 1, an aerobic bacteria continuous automatic cultivation system comprises a strain cultivation area 1;

a plurality of shaking platforms 4 which are connected with each other to form a production line are arranged in the strain cultivation area 1, and a plurality of push-pull conveying devices 3 drive the strain cultivation boxes 8 to walk along the production line of the shaking platforms 4 so as to finish strain cultivation. With the structure, continuous and automatic aerobic bacteria cultivation is realized through automatic stepping of the strain culture box 8. The time for the strain culture box 8 to travel from the head end to the tail end of the production line of the shaking platform 4 is the culture maturation time of the aerobic bacteria.

The preferable scheme is as shown in figures 5-7, the structure of the strain culture box 8 is as follows: the two sides of the box body 81 are provided with openable side wall structures, a culture blanket 82 is arranged in the box body 81, the inner surface of the culture blanket 82 is provided with a culture medium 88, the culture blanket 82 forms a water storage structure in the box body, and when the side wall of the box body 81 is opened, the two sides of the culture blanket 82 slide down to enable the bacteria liquid to flow out. With the structure, the strain culture box 8 can automatically collect the bacteria liquid after culture.

The preferable scheme is as shown in figures 5-7, the structure of the strain culture box 8 is as follows: the side wall of the box 81 is provided with a sliding slot 84, a box sliding side wall 86 is slidably mounted in the sliding slot 84, and the box sliding side wall 86 is connected with the box 81 through a tension spring 85, so that the box sliding side wall 86 is in a blocking state.

The bottom of the box body 81 is provided with an extended foot part, the height of the extended foot part is greater than or equal to that of the box body sliding side wall 86, the bottom of the box body sliding side wall 86 is provided with a deflector rod 83, and the deflector rod 83 is used for driving the box body sliding side wall 86 to slide downwards to be in an opening state; preferably, the end of the lever (83) is located outside the box sliding side wall 86 in a top plan view so as to be in contact with the opening guide 27.

A culture blanket 82 is arranged in the box body 81, a culture medium 88 is arranged on the inner surface of the culture blanket 82, the culture blanket 82 forms a water storage structure in the box body, and when the side wall of the box body 81 is opened, the two sides of the culture blanket 82 slide down to enable bacteria liquid to flow out. With the structure, the automatic collection of the bacteria liquid by pressing the deflector rod 83 down by the opening guide rail 27 after the culture of the bacteria culture box 8 is realized.

The preferable scheme is as shown in figure 1, a bacterial liquid collecting platform 7 is also arranged in the strain culture area 1;

one end of the bacteria liquid collecting platform 7 is provided with a push-pull conveying device 3 for driving the strain culture box 8 to walk;

the head end of the bacteria liquid collecting platform 7 is connected with the tail end of the shaking platform 4 production line;

an opening guide rail 27 is arranged on at least one side of the bacteria liquid collecting platform 7, the bottom of the opening guide rail 27 is in contact with a shifting rod 83, one end of the opening guide rail 27 close to the head end of the bacteria liquid collecting platform 7 is higher, the other end of the opening guide rail 27 is lower, and the opening guide rail 27 is used for pressing down the shifting rod 83 to enable a box body sliding side wall 86 to slide downwards to be in an opening state;

and a bacterium liquid collecting groove 5 is arranged below at least one side of the bacterium liquid collecting platform 7 and is used for collecting bacterium liquid. The bacteria liquid collecting tank 5 is of a chute structure, and a bacteria liquid box 6 is arranged at the lower end of the bacteria liquid collecting tank 5 and used for collecting bacteria liquid.

As shown in FIG. 6, the structure of culture blanket 82 is: the outer side or the inner side of the fabric is provided with a waterproof film;

the fabric is made of cotton cloth or chemical fiber, and the waterproof film is made of plastic film or agar. With the structure, the automatic flow of the bacteria liquid can be realized, and the bacteria liquid can be recycled after being cleaned and disinfected. Wherein the flexible agar is used as a waterproof membrane and can also be used as a nutrient substance.

In a preferred scheme, as shown in fig. 3 and 4, the push-pull conveying device 3 is structured in such a way that a push plate or a pull plate is arranged at the end of a piston rod of an air cylinder and is used for pushing and pulling one position of the strain culture box 8, wherein the one position refers to one length or width distance of the strain culture box 8.

In a preferred scheme, as shown in fig. 1, a plurality of shaking platforms 4 form an array structure, the tops of the shaking platforms 4 are flush, and one end of each row of the plurality of shaking platforms 4 is provided with a push-pull conveying device 3 for pushing, so as to push a plurality of strain culture boxes 8 to walk along the current row; the other end of the plurality of shaking platforms 4 in each row is provided with a push-pull conveying device 3 for side pulling or side pushing, which is used for changing the row of the strain culture boxes 8. In the above-described structure, the plurality of seed culture boxes 8 are made to continuously travel in an "S" shape along the array structure constituted by the rocking platform 4, and move from the head end to the tail end.

The preferred scheme is as shown in fig. 3 and 4, and the structure of the shaking platform 4 is as follows: the top of the support 43 is provided with a plurality of elastic elements 42, the top of the elastic elements 42 is fixedly connected with the flat plate 41, and the support 43 is provided with a cam driving mechanism 44 or a crank rocker mechanism 45 to drive the flat plate 41 to swing. The cam driving mechanism 44 is configured such that the motor drives the cam to rotate through the speed reducer, and the edge of the cam contacts the plate 41 to drive the plate 41 to shake.

In a preferred embodiment, as shown in FIG. 1, a temperature control device 11 is provided in the seed culture section 1;

the strain cultivation area 1 is further connected with an air supply device 13, an air sterilization and filtration device 12 is arranged at an inlet of the air supply device 13 and used for sending filtered air into the strain cultivation area 1 and the solid fermentation area 2, and the strain cultivation area 1 and the solid fermentation area 2 are in positive pressure. Preferably, a heating device is further arranged in the air sterilization filtering device 12 to assist sterilization, and a resistance wire or a PTC heating plate is usually arranged in the heating device to heat the temperature to 50-120 ℃ so as to heat the whole strain cultivation area 1 to 30-37 ℃. Meanwhile, the sterilization effect can be achieved.

In a preferred scheme, as shown in figure 1, a culture medium disinfection room 21 is arranged at an inlet of a strain culture area 1, and an array type inoculation device 19 is arranged in the culture medium disinfection room 21 and is used for inoculating a strain culture box 8; the bottom of the array type inoculation device 19 is provided with a plurality of nozzles arranged in an array, and pumped strains are sprayed out of the nozzles arranged in the array to be inoculated in the whole plane range of the strain culture box 8.

A push-pull conveying device 3 is arranged in the culture medium disinfection room 21 and is used for driving the strain culture box 8 to walk or enter the strain culture area 1;

a shutter 28 is provided between the medium sterilizing compartment 21 and the seed culture section 1. The shutter 28 is provided to effectively prevent air exchange between the culture sterilization booth 21 and the seed culture section 1, and normally, air flows from the seed culture section 1 to the culture sterilization booth 21.

Example 2:

on the basis of example 1, as shown in fig. 2, an automatic natto production system includes a seed culture section 1 and a solid fermentation section 2;

the structure of the seed culture section 1 was the same as in example 1. The moving time of the strain culture box 8 in the strain culture area 1 is 12-18 h. The temperature of the strain culture section 1 is controlled to 30 to 37 ℃.

As shown in fig. 1, the strain culture area 1 is provided with a strain liquid collection platform 7 for collecting strain liquid in a strain culture box 8; the collected inoculum is contained in the inoculum box 6 for transport to the inoculum box 26.

The solid fermentation area 2 is provided with a bacterial liquid inoculation box 26 for inoculating the beans in the bean fermentation box 15 with the collected bacterial liquid; the bacteria liquid inoculation chamber 26 is provided at a high position, and the bacteria liquid flows down by its own weight, and this structure is adopted to ensure the activity of the bacteria liquid by using as few pumps as possible as transportation means. An array type inoculation device 19 is arranged at the bottom of the bacteria liquid inoculation box 26, and a plurality of nozzles which are arranged in an array mode are arranged at the bottom of the array type inoculation device 19 and used for enabling strains to be evenly distributed on a plane, such as the whole plane of the bean fermentation box 15. The control of the inoculation amount of the bacteria liquid inoculation box 26 adopts weighing control, a weighing sensor is arranged at the bottom of the bacteria liquid inoculation box 26, and the inoculation amount is controlled by controlling the reduction of weight. The bacterium liquid is stopped by a flashboard controlled by a cylinder.

The solid fermentation area 2 is also internally provided with a plurality of fermentation platforms 25, the fermentation platforms 25 are connected with each other to form a production line, and the bean fermentation boxes 15 are driven to walk along the production line of the fermentation platforms 25 by a plurality of push-pull conveying devices 3. The walking mode of the bean fermentation box 15 in the solid fermentation area 2 is the same as that of the strain culture box 8 in the strain culture area 1. The time for the bean fermentation box 15 to finish the whole solid fermentation area 2 is 24-36 h. The temperature of the solid fermentation zone 2 is controlled to be 30-37 ℃.

In a preferred embodiment, as shown in fig. 6, a bacteria liquid box 6 is arranged below the bacteria liquid collecting groove 5 to collect bacteria liquid flowing down from the bacteria liquid collecting groove 5. The bacteria liquid box 6 is conveyed to the lifting device 9 through a conveying device and is used for injecting bacteria liquid into the bacteria liquid inoculation box 26, and an array type inoculation device 19 is arranged in the bacteria liquid inoculation box 26; a gate valve is arranged at the bottom of the bacteria liquid box 6, and when the lifting device 9 is lifted to the top, the gate valve is opened by an external collision block or a mechanical arm, so that the bacteria liquid is injected into the bacteria liquid inoculation box 26. In another optional scheme, a turnover platform is arranged at the top of the lifting device 9, and the bacteria liquid is directly poured into the bacteria liquid inoculation box 26.

Preferably, as shown in fig. 8, the conveying device includes a material transfer trolley 22, and the structure of the material transfer trolley 22 is as follows: the top of the moving vehicle body 223 is provided with a plurality of rollers 221, the top of the moving vehicle body 223 is provided with a driving chain 222, the driving chain 222 in this example is a chain transmission mechanism driven by a motor and a speed reducer, the driving chain 222 is located below the surfaces of the rollers 221, and the driving chain 222 is fixedly connected with a push plate 224 and used for pushing the box body on the top of the moving vehicle body 223 to other stations.

In a preferred embodiment, as shown in FIG. 2, the solid fermentation zone 2 is provided with a temperature control device 11;

the solid fermentation area 2 is also connected with an air supply device 13, an inlet of the air supply device 13 is provided with an air sterilization and filtration device 12 for sending filtered air into the strain cultivation area 1 and the solid fermentation area 2, and the strain cultivation area 1 and the solid fermentation area 2 are in positive pressure.

In a preferred embodiment, the array type inoculation device 19 is provided with a plurality of nozzles arranged in an array at the bottom of the array type inoculation device 19, so that strains are uniformly distributed on a plane. For example, uniformly distributed on the whole plane of the bean fermenting case 15.

In a preferred scheme, as shown in fig. 2, a bean sterilizing room 14 is arranged at the inlet of the solid fermentation area 2, and a push-pull conveying device 3 for conveying a bean fermentation box 15 is arranged in the bean sterilizing room 14; the sterilization mode adopts one or more of the modes of ultraviolet light, low-temperature high-pressure sterilization, microwave sterilization, radiation sterilization, high-temperature sterilization, electrostatic sterilization and the like, and a flashboard 28 is arranged between the bean sterilization room 14 and the solid fermentation area 2;

the solid fermentation zone 2 is also connected to an after-ripening device 23, and the after-ripening device 23 is connected to the crushing device 18. The conveying of the after-ripening device 23 adopts a conveying belt or the same conveying mode as the strain cultivation area 1, the retention time of the after-ripening device 23 is 24-36 h, and the environmental temperature is 4-8 ℃. Therefore, a low-temperature air conditioner is also required in the after-ripening apparatus 23. The smashing device 18 is used for smashing the mean value of the ripe beans and natto and then sending the smashed beans and natto to a sterilizing and packaging room 24 for freeze-drying and packaging.

Example 3:

the method of using the present invention will be described with reference to the most preferred embodiment based on example 2.

As shown in FIG. 2, before use, the seed culture section 1, the solid fermentation section 2, the medium sterilization chamber 21, the bean sterilization chamber 14, the after-ripening device 23, the crushing device 18 and the sterilization packing chamber 24 are sufficiently sterilized. The air supply device 13 is started preliminarily, the internal and external pressure difference between the strain cultivation area 1 and the solid fermentation area 2 is tested, whether the microorganisms contained in overflowed air exceed the standard or not is detected, the temperature control device 11 is started after the requirements are met, and the temperature of the strain cultivation area 1 and the temperature of the solid fermentation area 2 are controlled to be 30-37 ℃.

As shown in figure 2, the sterilized strain culture box 8 is fed into a culture medium disinfection room 21, bacteria are sterilized at the upstream of the array type inoculation device 19, when the strain culture box 8 is fed below the array type inoculation device 19, strains in the strain temporary storage kettle 20 and water are injected into the strain culture box 8 by the array type inoculation device 19, and the strain culture box 8 moves forwards continuously and is pushed to the shaking platform 4 of the strain culture area 1 by a push-pull conveying device 3. The shutter 28 is temporarily opened while the push-pull conveyor 3 is pushing. The push-pull conveying device 3 pushes the continuous strain culture boxes 8 to enter the strain culture area 1 to walk in an S shape. When the strain culture box 8 is moved to the tail end of the production line of the shaking platform 4, the strain culture box needs to spend 12-18 hours. In the time quantum that the culture box 8 is static, shake the platform 4 and start, drive the dull and stereotyped 41 and sway, preferably, adopt the crank rocker mechanism 45 by motor drive to drive culture box 8 and sway, because the area of culture box 8 is great, the velocity of water flow is higher, the speed that adopts 40~50 rotations after the reduction gear of motor speed reduction can satisfy the culture requirement. Because the positive pressure scheme is adopted in the strain culture area 1, the air contact area of the strain culture box 8 is large, the oxygen supply is sufficient, and the culture quality can be effectively improved compared with the prior art. The shaking time of the platform 4 is 5min each time, and the corresponding push-pull conveying device 3 is started once after the platform is stopped, so that one step of the strain culture box 8 is completed.

As shown in FIG. 2, at the end of the production line of the shaking platform 4, a push-pull conveyer 3 arranged at one side pulls the seed culture box 8 onto the bacteria liquid collection platform 7, and a push-pull conveyer 3 arranged at the top end of the bacteria liquid collection platform 7 pushes the seed culture box 8 forward continuously. When the bacteria liquid passes through the opening guide rail 27, as shown in fig. 4, the deflector rod 83 enters the lower part of the opening guide rail 27 and is gradually pressed down by the opening guide rail 27, the box body sliding side wall 86 descends to enable the two sides of the culture blanket 82 to hang down, the bacteria liquid 87 automatically flows into the bacteria liquid collecting tank 5 and flows into the bacteria liquid box 6 along the bacteria liquid collecting tank 5, when the bacteria liquid box 6 is full, the bacteria liquid box is detected by a video feedback or ultrasonic detection or a weighing mechanism of the material transfer trolley 22, the material transfer trolley 22 conveys the bacteria liquid box 6 to the conveying device 10, and the conveying device 10 adopts a conveying belt. Conveyor 10 carries fungus liquid box 6 to hoisting device 9, and hoisting device 9 adopts the lift platform, and hoisting device 9 top is equipped with the upset platform, sends into fungus liquid inoculation case 26 with the fungus liquid in the fungus liquid box 6 in. An array type inoculation device 19 is arranged at the bottom of the bacteria liquid inoculation box 26. The germinated beans are placed in a bean fermentation box 15, the beans are conveyed into a solid fermentation area 2 through a push-pull conveying device 3 after being sterilized in a bean sterilizing room 14, a flashboard 28 is opened during conveying, the bean fermentation box 15 is inoculated with bacterial liquid below a bacterial liquid inoculation box 26, and then the bean fermentation box 15 walks along a fermentation platform 25 in an S shape under the driving of the push-pull conveying device 3. At the tail end of the production line of the fermentation platform 25, the walking time of the bean fermentation box 15 is 24-36 h. The bean fermentation box 15 is conveyed to an after-ripening device 23 through a material transfer trolley 22, travels in the after-ripening device 23 for 24-36 hours, is conveyed into a crushing device 18 for homogenizing and crushing, is conveyed into a sterilizing packaging room 24 for freeze drying and packaging, and is filled with nitrogen in the packaging process to obtain the high-activity natto powder containing high-purity nattokinase and natto probiotics. The production process is completed in one closed space, so that the possibility of being infected by the mixed bacteria is greatly reduced, the content of harmful mixed bacteria such as aspergillus flavus and the like can be greatly reduced, the adopted bacteria liquid with higher purity can greatly improve the product quality and reduce uncomfortable flavor. Especially, unmanned automatic operation can be realized, the labor is greatly reduced, and the production cost is reduced. The whole production process of the invention does not adopt a pumping scheme, and the activity of the strain can be effectively improved.

The above-described embodiments are merely preferred embodiments of the present invention, and should not be construed as limiting the present invention, and features in the embodiments and examples in the present application may be arbitrarily combined with each other without conflict. The protection scope of the present invention is defined by the claims, and includes equivalents of technical features of the claims. I.e., equivalent alterations and modifications within the scope hereof, are also intended to be within the scope of the invention.

Claims

1. The utility model provides an aerobic bacteria continuous automation cultivation system which characterized by: it comprises a strain cultivation area (1);

a plurality of shaking platforms (4) which are connected with each other to form a production line are arranged in the strain cultivation area (1), and a plurality of push-pull conveying devices (3) are used for driving the strain cultivation boxes (8) to walk along the production line of the shaking platforms (4) so as to finish strain cultivation.

2. The continuous automatic aerobic bacteria cultivating system as claimed in claim 1, wherein: the structure of the strain culture box (8) is as follows: both sides of box body (81) are equipped with the lateral wall structure that can open and shut, are equipped with in box body (81) and cultivate blanket (82), and the internal surface of cultivation blanket (82) is equipped with culture medium (88), and cultivation blanket (82) constitute water storage structure in the box body, opens when the lateral wall of box body (81), and the both sides landing of cultivation blanket (82) is used for making the fungus liquid flow out.

3. The continuous automatic aerobic bacteria cultivating system as claimed in claim 1, wherein: the structure of the strain culture box (8) is as follows: the side wall of the box body (81) is provided with a sliding groove (84), the sliding side wall (86) of the box body is slidably arranged in the sliding groove (84), and the sliding side wall (86) of the box body is connected with the box body (81) through a tension spring (85) so that the sliding side wall (86) of the box body is in a blocking state;

the bottom of the box body (81) is provided with an extended foot part, the height of the extended foot part is greater than or equal to that of the box body sliding side wall (86), the bottom of the box body sliding side wall (86) is provided with a deflector rod (83), and the deflector rod (83) is used for driving the box body sliding side wall (86) to slide downwards to be in an opening state;

be equipped with in box body (81) and cultivate blanket (82), cultivate blanket (82) internal surface and be equipped with culture medium (88), cultivate blanket (82) and constitute water storage structure in the box body, open when the lateral wall of box body (81), the both sides landing of cultivation blanket (82) is used for making the fungus liquid flow out.

4. The continuous automatic aerobic bacteria cultivating system as claimed in claim 3, wherein: a bacterial liquid collecting platform (7) is also arranged in the strain cultivation area (1);

one end of the bacteria liquid collecting platform (7) is provided with a push-pull conveying device (3) for driving the strain culture box (8) to walk;

the head end of the bacteria liquid collecting platform (7) is connected with the tail end of the production line of the shaking platform (4);

an opening guide rail (27) is arranged on at least one side of the bacteria liquid collecting platform (7), the bottom of the opening guide rail (27) is in contact with a shifting rod (83), one end of the opening guide rail (27), which is close to the head end of the bacteria liquid collecting platform (7), is higher, the other end of the opening guide rail is lower, and the opening guide rail is used for pressing down the shifting rod (83) to enable a sliding side wall (86) of the box body to slide downwards to be in an opening state;

a bacterium liquid collecting groove (5) is arranged below at least one side of the bacterium liquid collecting platform (7) and is used for collecting bacterium liquid.

5. The continuous automatic aerobic bacteria cultivating system as claimed in claim 2 or 3, wherein: the structure of the culture blanket (82) is as follows: the outer side or the inner side of the fabric is provided with a waterproof film;

6. The continuous automatic aerobic bacteria cultivating system as claimed in claim 1, wherein: the push-pull conveying device (3) is structurally characterized in that a push plate or a pull plate is arranged at the end of a piston rod of the air cylinder and used for pushing and pulling one position of the strain culture box (8), and the one position refers to one length or width distance of the strain culture box (8).

7. The continuous automatic aerobic bacteria cultivating system as claimed in claim 6, wherein: the plurality of shaking platforms (4) form an array structure, the tops of the shaking platforms (4) are parallel and level, and one end of each row of the plurality of shaking platforms (4) is provided with a push-pull conveying device (3) for pushing, and the push-pull conveying device is used for pushing the plurality of strain culture boxes (8) to walk along the current row; the other end of each row of the plurality of shaking platforms (4) is provided with a push-pull conveying device (3) for side pulling or side pushing, and the push-pull conveying device is used for changing the rows of the strain culture boxes (8).

8. The continuous automatic aerobic bacteria cultivating system as claimed in claim 1, wherein: the structure of the shaking platform (4) is as follows: the top of the support (43) is provided with a plurality of elastic elements (42), the tops of the elastic elements (42) and the flat plate (41) are fixedly connected, and the support (43) is provided with a cam driving mechanism (44) or a crank rocker mechanism (45) to drive the flat plate (41) to swing.

9. The continuous automatic aerobic bacteria cultivating system as claimed in claim 1, wherein: the strain cultivation area (1) is provided with a temperature control device (11);

the strain cultivation area (1) is also connected with an air supply device (13), an air sterilization and filtration device (12) is arranged at the inlet of the air supply device (13) and used for sending filtered air into the strain cultivation area (1) and the solid fermentation area (2), and the strain cultivation area (1) and the solid fermentation area (2) are in positive pressure.

10. The continuous automatic aerobic bacteria cultivating system as claimed in claim 1, wherein: a culture medium disinfection room (21) is arranged at the inlet of the strain culture area (1), and an array inoculation device (19) is arranged in the culture medium disinfection room (21) and is used for inoculating the strain culture box (8);

a push-pull conveying device (3) is arranged in the culture medium disinfection room (21) and is used for driving the strain culture box (8) to walk or enter the strain culture area (1);

a flashboard (28) is arranged between the culture medium disinfection room (21) and the strain cultivation area (1).