CN112294936A - Culture medium for preparing stomach-protecting fermentation product and preparation method - Google Patents
Culture medium for preparing stomach-protecting fermentation product and preparation method Download PDFInfo
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Abstract
The invention discloses a culture medium for preparing a stomach-protecting fermentation product and a preparation method thereof, wherein the culture medium comprises the following components in parts by weight: 10-15 parts of sea buckthorn pulp, 10-15 parts of clove extract, 10-15 parts of fine yam powder, 3-5 parts of extracts of bamboo fungus, ganoderma applanatum and phellinus igniarius, 1-2 parts of fine turmeric powder, 3-6 parts of galacto-oligosaccharide and 80-100 parts of water. The preparation method comprises inoculating activated Hericium Erinaceus strain into seed liquid culture medium, and performing fermentation culture for 3-7 days to obtain Hericium Erinaceus liquid seed liquid; then, the hericium erinaceus liquid seed liquid accounting for 2% -5% of the volume of the culture medium is inoculated into the culture medium, the hericium erinaceus liquid seed liquid is cultured for 5-8 days at the temperature of 24-27 ℃, after fermentation is finished, fermentation liquor is subjected to vacuum freeze drying, and a stomach-protecting fermentation product is obtained. The prepared hericium erinaceus fermentation product has an obvious protective effect on gastritis and gastric ulcer induced by ethanol.
Description
Technical Field
The invention relates to a culture medium for preparing a stomach-protecting fermentation product and a preparation method thereof.
Background
The biological product prepared by the biological fermentation technology is the basis for overcoming the best way of utilizing wild resources with limited biomass and realizing the development and utilization of useful metabolites. Compared with the traditional sporocarp cultivation, the secondary metabolite is prepared by liquid fermentation, the production period is short, the metabolite is easy to control, and the large-scale production can be realized.
Chinese patent document CN104000192A discloses a functional food for relieving pressure and regulating intestines and stomach and a two-step fermentation preparation method thereof, the method comprises: taking natural components containing the components for regulating intestines and stomach as a medicinal culture medium, inoculating hericium erinaceus liquid strains to perform bidirectional solid fermentation to prepare medicinal mycoplasm with the function of regulating intestines and stomach; taking medicinal mycoplasm as a raw material, crushing the medicinal mycoplasm, adding yeast powder, sodium glutamate and water to prepare a fermentation medium, inoculating lactobacillus to perform liquid submerged fermentation to produce gamma-aminobutyric acid capable of relieving pressure, and preparing fermentation liquor capable of relieving pressure and regulating intestines and stomach; the fermentation liquor is adopted to prepare functional foods with different dosage forms for relieving pressure and regulating intestines and stomach. The functional food for relieving pressure and regulating intestines and stomach prepared by the method disclosed by the invention has the functions of relieving pressure and regulating intestines and stomach by matching the hericium erinaceus mycoplasm and the gamma-aminobutyric acid, has a wide market prospect, is stable in preparation method and is suitable for large-scale production. The invention mainly aims to produce the gamma-aminobutyric acid capable of relieving pressure through fermentation, so that the hericium erinaceus has the function of regulating intestines and stomach, the gamma-aminobutyric acid has the function of relieving pressure, and the combination of the gamma-aminobutyric acid and the hericium erinaceus has the functions of relieving pressure and regulating intestines and stomach.
Chinese patent document CN102172270A discloses a polysaccharide granule for conditioning and health care of stomach and intestine, which is prepared by the following steps: a. the hericium erinaceus fermentation takes wort as a fermentation medium, the sugar degree of the wort is 5-20 ℃, hericium erinaceus cells are used as strains, the weight ratio of the inoculation amount of the wort to the inoculation amount of the hericium erinaceus cells is 99-90: 1-10, the fermentation temperature is 25-30 ℃, the pH value is adjusted to 4-7, and the fermentation time is 12-48 hours; b. obtaining a hericium erinaceus polysaccharide concentrated solution by membrane filtration and concentration, wherein a ceramic membrane filter is adopted, and the aperture is 0.2 um; c. atomizing the hericium erinaceus polysaccharide concentrated solution in a drying room, and then rapidly vaporizing water in contact with hot air to obtain hericium erinaceus polysaccharide powder; the air inlet temperature is 155-185 ℃, the air exhaust temperature is 80-100 ℃, the air exhaust pressure is 100-150 Pa, and the atomization frequency is 25 HZ; d. and fully and uniformly mixing 5-15 parts by weight of hericium erinaceus polysaccharide powder, 5-15 parts by weight of hawthorn powder, 5-25 parts by weight of inulin, 45-65 parts by weight of fructo-oligosaccharide powder and 5-15 parts by weight of xylo-oligosaccharide powder to obtain a polysaccharide granule finished product. The hericium erinaceus polysaccharide powder is obtained by taking wort as a fermentation medium and taking hericium erinaceus cells as strains and performing fermentation culture. The formula obtained by compounding the hericium erinaceus polysaccharide with the hawthorn powder, the inulin, the fructo-oligosaccharide and the xylo-oligosaccharide has unique gastrointestinal conditioning effect, and is a novel health-care solid drink.
Chinese patent document CN102389540A discloses a spleen and stomach strengthening calcium gluconate oral liquid, which comprises traditional Chinese medicine raw juice, calcium gluconate and a third formula; the raw material medicaments of the traditional Chinese medicine raw juice are as follows: 300-700 g of scorched hawthorn, 100-300 g of Chinese date, 50-120 g of roasted malt, 30-100 g of poria cocos, 30-100 g of clove, 10-60 g of Chinese yam, 10-40 g of dried orange peel, 10-40 g of sea-buckthorn and 10-40 g of honey; adding calcium gluconate into the decoction of the raw material medicine, and adding other ingredients: sucrose, citric acid, sodium chloride, potassium sorbate, sodium cyclamate, vitamins and healthy sugar. The zinc and iron in the invention are easy to be absorbed by human body. Not only can fully supplement trace elements necessary for human body, but also can promote excretion of harmful element lead accumulated in human body, and has good therapeutic effect. Although clove, Chinese yam and sea buckthorn are added into the traditional Chinese medicine, the traditional Chinese medicine is not used as a main component of raw juice of the traditional Chinese medicine and is not used as a culture medium.
Disclosure of Invention
In view of the above, the present invention aims to provide a culture medium for preparing a stomach-protecting fermented product and a preparation method thereof, wherein the prepared stomach-protecting fermented product has a significant protective effect on gastritis and gastric ulcer induced by ethanol through fermentation culture using the culture medium.
The adopted technical scheme is as follows:
the invention relates to a culture medium for preparing a stomach-protecting fermentation product, which comprises the following components in parts by weight: 10-15 parts of sea buckthorn pulp, 10-15 parts of clove extract, 10-15 parts of fine yam powder, 3-5 parts of extracts of bamboo fungus, ganoderma applanatum and phellinus igniarius, 1-2 parts of fine turmeric powder, 3-6 parts of galacto-oligosaccharide and 80-100 parts of water.
Further, the sea buckthorn fruit pulp is prepared by the following method: removing stem from fresh fructus Hippophae, removing rotten fruit, washing with water, draining, and squeezing to obtain juice.
Further, the clove extract is prepared by the following method: pulverizing flos Caryophylli, adding 20-30 times of water, extracting in shaking table at 40-50 deg.C for 10-14 hr, centrifuging to obtain supernatant, concentrating the supernatant under reduced pressure, and vacuum freeze drying to obtain flos Caryophylli extract.
Further, the extracts of bamboo fungus, Ganoderma Applanatum and Microporus gracilis are prepared by the following method: respectively pulverizing bamboo fungus, Ganoderma Applanatum and Botrytis Tenaci, mixing at a weight ratio of 1: 0.5: 1, adding 20-30 times of 20 vol% ethanol solution, extracting in shaking table at 40-50 deg.C for 10-14 hr, filtering to obtain supernatant, concentrating the supernatant under reduced pressure, and vacuum freeze drying to obtain extracts of bamboo fungus, Ganoderma Applanatum and Botrytis Tenaci.
In the technical scheme, the sea-buckthorn pulp has the effects that the sea-buckthorn pulp has a good healing promoting effect on gastric ulcer, the beta-sitosterol-beta-D-glucoside in the sea-buckthorn pulp is an anti-gastric ulcer effective component, gastric mucosa can be effectively protected, gastric acid secretion can be inhibited, the damage degree of the gastric mucosa is reduced, and the sea-buckthorn pulp also has the effects of resisting inflammation, promoting tissue regeneration and promoting ulcer healing.
In the technical scheme, the clove extract has the functions of increasing gastric secretion, resisting gastric ulcer, stopping diarrhea, benefiting gallbladder, resisting inflammation, easing pain, resisting anoxia, resisting blood coagulation and inhibiting bacteria.
In the technical scheme, the fine yam powder has the effects of protecting and nourishing the stomach and can provide a carbon source for fermentation of the hericium erinaceus.
The turmeric fine powder has the function of relieving stomachache.
The bamboo fungus extract is commonly used for treating pharyngolaryngitis, gastritis, gastric ulcer, tonsillitis and the like, and has the function of resisting gastritis and gastric ulcer in the stomach-protecting fermentation product. Ganoderma Applanatum extract and Microporus gracilis extract have functions of treating gastritis and gastric ulcer.
Galactooligosaccharides (GOS) are functional oligosaccharides having natural properties, and are excellent nutritional sources and effective proliferation factors for beneficial bacteria such as bifidobacterium, lactobacillus acidophilus and the like in human intestinal tracts, and can improve the digestion and absorption functions of the human intestinal tracts.
The invention relates to a preparation method of a stomach-protecting fermentation product, which comprises the following steps:
(1) inoculating the activated Hericium erinaceus strain into seed liquid culture medium, and performing fermentation culture for 3-7 days to obtain Hericium erinaceus liquid seed liquid;
(2) inoculating the hericium erinaceus liquid seed liquid accounting for 2-5% of the volume of the culture medium into the culture medium, culturing for 5-8 days at 24-27 ℃, finishing fermentation, and carrying out vacuum freeze drying on the fermentation liquor to obtain the stomach-protecting fermentation product.
Further, in the step (1), the seed liquid culture medium comprises the following components in parts by weight: 20 parts of potato, 2 parts of glucose, 0.05 part of magnesium sulfate and 0.02 part of monopotassium phosphate.
Further, in the step (1), the hericium erinaceus strain is selected from the dichroa febrifuga 99.
In the technical scheme, active substances are organically combined or recombined by utilizing hericium erinaceus liquid fermentation, and the hericium erinaceus has the functions of tonifying spleen and nourishing stomach and treating digestive tract diseases such as gastric ulcer, chronic gastritis and the like.
The stomach-protecting fermentation product is prepared by the preparation method.
In conclusion, the invention has the advantages that,
(1) the nutrient components and the effective components in the fermentation culture solution are better biodegraded, converted and recombined through fermentation, and the stomach protecting activity of the final product is improved.
(2) The prepared hericium erinaceus fermentation product has an obvious protective effect on gastritis and gastric ulcer induced by ethanol.
Drawings
FIG. 1 is a map of a pathological section of mouse stomach tissue.
In the figure, Ethanol represents Ethanol. The fermented product is stomach-protecting fermented product.
Detailed Description
The present invention is described in detail below with reference to specific examples, but the use and purpose of these exemplary embodiments are merely to exemplify the present invention, and do not set forth any limitation on the actual scope of the present invention in any form, and the scope of the present invention is not limited thereto.
Example 1
A culture medium for preparing a stomach-protecting fermentation product is prepared from the following raw materials in parts by weight:
10 portions of sea-buckthorn fruit pulp
10 parts of clove extract
10 parts of fine yam powder
3 parts of extracts of bamboo fungus, ganoderma applanatum and phellinus igniarius
Turmeric fine powder 1 part
Galacto-oligosaccharide 3 parts
80 parts of water.
The preparation method of the stomach-protecting fermentation product comprises the following steps:
s1, inoculating the activated hericium erinaceus strain into a seed liquid culture medium to perform hericium erinaceus fermentation culture for 3-7 days to obtain a hericium erinaceus liquid seed liquid;
s2, inoculating a hericium erinaceus liquid seed solution accounting for 2% -5% of the volume of the culture medium into the culture medium, culturing for 5-8 days at the temperature of 24-27 ℃, finishing fermentation, and carrying out vacuum freeze drying on fermentation liquor to obtain a stomach-protecting fermentation product.
Example 2
A culture medium for preparing a stomach-protecting fermentation product is prepared from the following raw materials in parts by weight:
sea-buckthorn fruit pulp 12 parts
Clove extract 12 parts
10 parts of fine yam powder
4 parts of extracts of bamboo fungus, ganoderma applanatum and phellinus igniarius
2 parts of turmeric fine powder
Galacto-oligosaccharide 3 parts
And 90 parts of water.
The preparation method of the stomach-protecting fermentation product comprises the following steps:
s1 inoculating the activated Hericium erinaceus strain into a seed liquid culture medium, and performing fermentation culture for 3-7 days to obtain Hericium erinaceus liquid seed liquid;
s2 inoculating liquid seed liquid of Hericium erinaceus (2-5 vol.% of the culture medium) into the culture medium, culturing at 24-27 deg.C for 5-8 days, and vacuum freeze drying the fermented liquid to obtain fermented product for protecting stomach.
Example 3
A culture medium for preparing a stomach-protecting fermentation product is prepared from the following raw materials in parts by weight:
sea-buckthorn fruit pulp 15 parts
Clove extract 15 parts
10 parts of fine yam powder
5 parts of extracts of bamboo fungus, ganoderma applanatum and leptinotus
2 parts of turmeric fine powder
Galacto-oligosaccharide 3 parts
100 portions of water
The preparation method of the stomach-protecting fermentation product comprises the following steps:
s1 inoculating the activated Hericium erinaceus strain into a seed liquid culture medium, and performing fermentation culture for 3-7 days to obtain Hericium erinaceus liquid seed liquid;
s2 inoculating liquid seed liquid of Hericium erinaceus (2-5 vol.% of the culture medium) into the culture medium, culturing at 24-27 deg.C for 5-8 days, and vacuum freeze drying the fermented liquid to obtain fermented product for protecting stomach.
Example 4
Animal experiments
50 ICR mice with weight of 18-22 g are randomly divided into a model control group, a magnesium aluminocarbonate sheet (represented by magnesium aluminum carbonate in figure 1) positive control group (0.78g/kg) and a three-dose group (3.5, 1.75 and 0.875g/kg) of the fermentation product of the stomach protection of any one of the examples 1-3, 10 mice in each group are respectively subjected to stomach irrigation according to 10ml/kg of weight to be respectively administered with corresponding medicaments, the stomach irrigation is performed for 1 time per day for 8 days, and the model control group is subjected to stomach irrigation with distilled water with the same volume. Fasting is not forbidden for 24h before the last administration, absolute ethyl alcohol (represented by Ethanol in figure 1) is 0.1 ml/per stomach after 2h after the last administration, the mice are killed by dislocation of cervical vertebrae 1h after the model is made, the whole stomach is cut off after the abdomen is opened, the stomach is cut off along the greater curvature, dirt on the surface of gastric mucosa is slightly washed by brine ice, mucus on the surface of gastric mucosa is completely removed by sucking dry by filter paper, partial gastric tissue is homogenized and centrifuged, and the supernatant is taken for low-temperature freezing storage of the sample. And fixing another part of the gastric formaldehyde, performing HE staining, and performing pathological observation.
Results of the experiment
See table 1 and fig. 1 for experimental results.
Table 1 shows the effect of the gastroprotected fermentation products on the total SOD activity in gastric tissues of gastric ulcer with gastric mucosa injury in mice induced by absolute ethanol (x ± s, n ═ 10).
TABLE 1
Note: p <0.05, p <0.01 (compared to model control group)
As can be seen from Table 1, the gastric protection fermentation product significantly improves the SOD activity of mouse gastric tissue, and the high-dose gastric protection fermentation product significantly reduces the MDA content of mouse gastric tissue, and the result shows that the gastric protection fermentation product has very strong gastric protection activity.
As can be seen from figure 1, ethanol can cause swelling of mouse mucous membrane, infiltration of submucosa white cells, hemorrhagic injury and epithelial cell deletion, and the stomach protection fermentation product can relieve the stomach injury to a great extent.
The above-listed detailed description is only a specific description of a possible embodiment of the present invention, and they are not intended to limit the scope of the present invention, and equivalent embodiments or modifications made without departing from the technical spirit of the present invention should be included in the scope of the present invention.
Claims (8)
1. A culture medium for preparing a stomach-protecting fermentation product is characterized by comprising the following components in parts by weight: 10-15 parts of sea buckthorn pulp, 10-15 parts of clove extract, 10-15 parts of fine yam powder, 3-5 parts of extracts of bamboo fungus, ganoderma applanatum and phellinus igniarius, 1-2 parts of fine turmeric powder, 3-6 parts of galacto-oligosaccharide and 80-100 parts of water.
2. The medium for preparing a stomach-protecting fermentation product according to claim 1, wherein the sea buckthorn berry is prepared by the following method: removing stem from fresh fructus Hippophae, removing rotten fruit, washing with water, draining, and squeezing to obtain juice.
3. The medium for preparing a gastroprotected fermentation product according to claim 1, wherein the clove extract is prepared by the following method: pulverizing flos Caryophylli, adding 20-30 times of water, extracting in shaking table at 40-50 deg.C for 10-14 hr, centrifuging to obtain supernatant, concentrating the supernatant under reduced pressure, and vacuum freeze drying to obtain flos Caryophylli extract.
4. The medium for preparing a stomach-protecting fermented product according to claim 1, wherein the extracts of bamboo fungus, Ganoderma applanatum and Microporus gracilis are prepared by the following method: respectively pulverizing bamboo fungus, Ganoderma Applanatum and Botrytis Tenaci, mixing at a weight ratio of 1: 0.5: 1, adding 20-30 times of 20 vol% ethanol solution, extracting in shaking table at 40-50 deg.C for 10-14 hr, filtering to obtain supernatant, concentrating the supernatant under reduced pressure, and vacuum freeze drying to obtain extracts of bamboo fungus, Ganoderma Applanatum and Botrytis Tenaci.
5. A preparation method of a stomach-protecting fermentation product is characterized by comprising the following steps:
(1) inoculating the activated Hericium erinaceus strain into seed liquid culture medium, and performing fermentation culture for 3-7 days to obtain Hericium erinaceus liquid seed liquid;
(2) the culture medium of any one of claims 1 to 4, wherein the hericium erinaceus liquid seed solution accounting for 2 to 5 percent of the volume of the culture medium is inoculated firstly, the hericium erinaceus liquid seed solution is cultured for 5 to 8 days at the temperature of 24 to 27 ℃, after the fermentation is finished, the fermentation liquid is subjected to vacuum freeze drying to obtain the stomach-protecting fermentation product.
6. The method for preparing a stomach-protecting fermentation product according to claim 5, wherein in the step (1), the seed liquid culture medium comprises the following components in parts by weight: 20 parts of potato, 2 parts of glucose, 0.05 part of magnesium sulfate and 0.02 part of monopotassium phosphate.
7. The method for preparing a stomach-protecting fermented product according to claim 5, wherein in the step (1), the Hericium erinaceus species is selected from Hericium erinaceus 99.
8. A fermented product for protecting stomach, which is produced by the production method according to any one of claims 5 to 7.
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