CN112274635A - Liquid feed additive for solving intestinal problems of animals and preparation method thereof - Google Patents
Liquid feed additive for solving intestinal problems of animals and preparation method thereof Download PDFInfo
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Abstract
The invention provides a liquid feed additive for solving the intestinal problem of animals, which comprises the following components in percentage by mass: 15-25% of rheum officinale, 5-8.3% of astragalus membranaceus, 10-22% of wrinkled gianthyssop herb, 3.5-7.1% of rhizoma atractylodis, 7.2-9.5% of bletilla striata and the balance lysozyme. The preparation method comprises four steps of traditional Chinese medicine extraction, purification operation, mother liquor preparation, packaging operation and the like. On one hand, the invention has simple preparation process and wide raw material source, thereby greatly reducing the cost of raw materials and processing operation; on the other hand, the feed additive has small toxic and side effects, can effectively balance animal intestinal flora, inhibit the reproduction and growth of harmful bacteria, kill the harmful bacteria, simultaneously adjust the balance of the intestinal flora, and promote the digestion and absorption of animals to the feed, thereby effectively solving the problems of diarrhea and poor nutrient absorption of the animals caused by intestinal diseases, and simultaneously, assisting in achieving the purposes of increasing the weight and the yield of the animals, improving the breeding efficiency, and reducing the breeding cost and the risk.
Description
Technical Field
The invention relates to a liquid feed additive for solving the intestinal problem of animals and a preparation method thereof, belonging to the technical field of traditional Chinese medicine biological preparations.
Background
In livestock breeding, because the balance of intestinal flora of animals is broken, and harmful bacteria are bred and propagated, digestive system diseases of animals are easily caused, the absorption, transportation and transformation capacity of nutrient components of feed are seriously influenced, so that the growth and development speed of the livestock is slow, even the livestock is subjected to digestive system diseases such as diarrhea and the like in serious cases, and the life safety of the livestock is seriously threatened, so that the breeding risk and the cost are greatly increased, and aiming at the problem, although various medicines for treating the digestive system diseases and stopping dysentery of the animals exist at present, the medicines generally adopt western medicine antibiotics or single enzyme preparations or traditional Chinese medicine powder, the effects of the products are single, only a small part of intestinal problems can be solved, the effects are not obvious, and in use, the antibiotic products can kill the intestinal harmful bacteria and beneficial bacteria and simultaneously cause the imbalance of the intestinal flora, and a large amount of drug residues are left, so that the method is not suitable for the requirements of green culture and food health development.
Therefore, in order to solve the problem, the development of a novel medicament for regulating the intestinal tract of animals is urgently needed so as to meet the requirement of actual breeding work.
In order to achieve the purpose, the invention provides the following technical scheme:
a liquid feed additive for solving the intestinal problem of animals comprises the following components in percentage by mass: 15-25% of rheum officinale, 5-8.3% of astragalus membranaceus, 10-22% of wrinkled gianthyssop herb, 3.5-7.1% of rhizoma atractylodis, 7.2-9.5% of bletilla striata and the balance lysozyme.
Furthermore, the rhubarb, the astragalus, the agastache, the rhizoma atractylodis and the bletilla are all Chinese medicinal decoction pieces.
A preparation method of a liquid feed additive for solving the intestinal problem of animals comprises the following steps:
s1, extracting Chinese medicine, storing rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata, cooling to-10-0 ℃, keeping the temperature, then keeping the temperature for 5-25 minutes, then ultrasonic crushing the rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata to obtain powder with the particle size of 5-10 μm, then adding the powder of rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata into alcohol water solution with the temperature of 20-45 ℃ and the concentration of 60-75% to soak for 30-120 minutes to obtain mixed liquid, then performing supercritical fluid extraction on the mixed liquid, performing membrane extraction and separation on the extracted residues, finally collecting and mixing the supercritical fluid extract and the separated liquid after membrane extraction, performing reduced pressure concentration on the mixed liquid to make the volume of the concentrated liquid 70-90% of the original volume, thus obtaining the extract liquid of rhubarb, astragalus, wrinkled gianthyssop herb, rhizoma atractylodis and bletilla striata, and collecting and processing the residue after removal;
s2, purifying, namely naturally cooling the extract liquor of the rhubarb, the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata obtained by the preparation in the step S1 to room temperature, adding ethanol into the extract liquor until the alcohol degree of the extract liquor reaches 60%, carrying out alcohol precipitation, taking supernate after the alcohol precipitation is finished, carrying out reduced pressure alcohol recovery on the supernate until the ethanol content in the supernate is not more than 3%, carrying out alcohol precipitation on the supernate, carrying out secondary alcohol precipitation, collecting the supernate obtained after the secondary alcohol precipitation, carrying out reduced pressure alcohol recovery for later use, carrying out solid-liquid separation, centrifugal separation and ionic membrane extraction and separation on the supernate obtained after the secondary alcohol precipitation, filtering by using a 0.22-micron microporous filter, collecting the filtered filtrate for later use, and simultaneously mixing and collecting residues obtained in the operation with the residue obtained in the step S1;
s3, preparing a mother solution, adding lysozyme into deionized water with the volume at least 5 times that of the lysozyme, adjusting the temperature of a lysozyme aqueous solution to 15-40 ℃ after the lysozyme is fully dissolved, and adjusting the pH value to 5.3-6.4, and finally adding the extraction liquid of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla obtained by purification in the step S2 into the lysozyme aqueous solution in sequence and mixing uniformly, wherein the adding speed of the extraction liquid of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla is 0.1-0.5 m/min, and the adding time interval of two adjacent extraction liquid is 3-15 minutes;
and S4, packaging, namely adding the mixed liquid obtained in the step S3 into a dark glass bottle through canning equipment, sealing, and storing in an environment which is protected from light and has the temperature of not more than 20 ℃ for later use.
Further, in the step S1, the volumes of the alcohol aqueous solutions for soaking are respectively 3 to 10 times of the volumes of the powders to be soaked.
Furthermore, the residues in the steps S2 and S3 are collected together and then added into a fermentation device for fermentation operation, and the fermented mixture is used as a feed raw material.
On one hand, the invention has simple preparation process and wide raw material source, thereby greatly reducing the cost of raw materials and processing operation; on the other hand, the feed additive has small toxic and side effects, can effectively balance animal intestinal flora, inhibit the reproduction and growth of harmful bacteria, kill the harmful bacteria, simultaneously adjust the balance of the intestinal flora, and promote the digestion and absorption of animals to the feed, thereby effectively solving the problems of diarrhea and poor nutrient absorption of the animals caused by intestinal diseases, and simultaneously, assisting in achieving the purposes of increasing the weight and the yield of the animals, improving the breeding efficiency, and reducing the breeding cost and the risk.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of the manufacturing process of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A liquid feed additive for solving the intestinal problem of animals comprises the following components in percentage by mass: 15% of rhubarb, 5% of astragalus, 10% of wrinkled gianthyssop herb, 3.5% of rhizoma atractylodis, 7.2% of bletilla and the balance of lysozyme.
Wherein, the rhubarb, the astragalus, the wrinkled gianthyssop, the rhizoma atractylodis and the bletilla are all Chinese herbal pieces.
A preparation method of a liquid feed additive for solving the intestinal problem of animals comprises the following steps:
s1, extracting Chinese medicine, storing rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata respectively, cooling to-10 ℃ and keeping the temperature, then keeping the temperature for 5 minutes, then respectively carrying out ultrasonic crushing on the rhubarb, the astragalus root, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata to prepare powder with the particle size of 5 mu m, then respectively adding the powder of the rhubarb, the astragalus root, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata into alcohol aqueous solution with the temperature of 20 ℃ and the concentration of 60 percent to be soaked for 30 minutes to obtain mixed solution, then carrying out supercritical fluid extraction on the mixed solution firstly, then carrying out membrane extraction separation on the extracted residues, finally collecting and mixing the supercritical fluid extract and the separated solution after the membrane extraction separation, and carrying out reduced pressure concentration on the mixed solution to ensure that the volume of the concentrated solution is 90 percent of the original volume, extracting the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata, and collecting and treating the residues after the extraction;
s2, purifying, namely naturally cooling the extract liquor of the rhubarb, the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata obtained by the preparation in the step S1 to room temperature, adding ethanol into the extract liquor until the alcohol degree of the extract liquor reaches 60%, carrying out alcohol precipitation, taking supernate after the alcohol precipitation is finished, carrying out reduced pressure alcohol recovery on the supernate until the ethanol content in the supernate is 3%, carrying out alcohol precipitation on the supernate, carrying out secondary alcohol precipitation, collecting the supernate obtained after the secondary alcohol precipitation, carrying out reduced pressure alcohol recovery for later use, filtering the supernate obtained after the secondary alcohol precipitation through a 0.22-micron microporous filter after solid-liquid separation, centrifugal separation and ionic membrane extraction and separation, collecting the filtered filtrate for later use, and simultaneously mixing and collecting residues obtained in the operation with residues obtained in the step S1;
s3, preparing a mother solution, adding lysozyme into deionized water with the volume 5 times that of the lysozyme, adjusting the temperature of a lysozyme aqueous solution to 15 ℃ after the lysozyme is fully dissolved, and adjusting the pH value to 5.3, and finally adding the extraction liquids of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla striata obtained by purification in the step S2 into the lysozyme aqueous solution in sequence and mixing uniformly, wherein the adding speed of the extraction liquids of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla striata is 0.1m/min, and the adding time interval of two adjacent extraction liquids;
and S4, packaging, namely adding the mixed liquid obtained in the step S3 into a dark glass bottle through canning equipment, sealing, and storing in an environment which is protected from light and has the temperature of not more than 20 ℃ for later use.
In step S1, the volumes of the alcohol aqueous solutions for soaking are respectively 3 times the volumes of the powders soaked in the alcohol aqueous solutions.
Meanwhile, the residues in the steps S2 and S3 are collected together and then added into a fermentation device for fermentation operation, and the fermented mixture is used as a feed raw material.
Example 2
A liquid feed additive for solving the intestinal problem of animals comprises the following components in percentage by mass: 25% of rhubarb, 8.3% of astragalus, 22% of wrinkled gianthyssop herb, 7.1% of rhizoma atractylodis, 9.5% of bletilla striata and the balance lysozyme.
Wherein, the rhubarb, the astragalus, the wrinkled gianthyssop, the rhizoma atractylodis and the bletilla are all Chinese herbal pieces.
A preparation method of a liquid feed additive for solving the intestinal problem of animals comprises the following steps:
s1, extracting Chinese medicine, storing rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata respectively, cooling to 0 ℃, keeping the temperature, then keeping the temperature for 25 minutes, then respectively carrying out ultrasonic crushing on the rhubarb, the astragalus root, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata to prepare powder with the particle size of 10 mu m, then respectively adding the powder of the rhubarb, the astragalus root, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata into alcohol aqueous solution with the temperature of 45 ℃ and the concentration of 75 percent to be soaked for 120 minutes to obtain mixed solution, then carrying out supercritical fluid extraction on the mixed solution firstly, carrying out membrane extraction separation on the residue after extraction, finally collecting and mixing the supercritical fluid extract and the separated solution after membrane extraction separation, and carrying out reduced pressure concentration on the mixed solution to ensure that the volume of the concentrated solution is 70 percent of the original volume, extracting the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata, and collecting and treating the residues after the extraction;
s2, purifying, namely naturally cooling the extract liquor of the rhubarb, the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata obtained by the preparation in the step S1 to room temperature, adding ethanol into the extract liquor until the alcohol degree of the extract liquor reaches 60%, carrying out alcohol precipitation, taking supernate after the alcohol precipitation is finished, carrying out reduced pressure alcohol recovery on the supernate until the ethanol content in the supernate is 0, carrying out alcohol precipitation on the supernate, carrying out secondary alcohol precipitation, collecting the supernate obtained after the secondary alcohol precipitation, carrying out reduced pressure alcohol recovery for later use, filtering the supernate obtained after the secondary alcohol precipitation through a 0.22-micron microporous filter after solid-liquid separation, centrifugal separation and ionic membrane extraction and separation, collecting the filtered filtrate for later use, and simultaneously mixing and collecting residues obtained in the operation with residues obtained in the step S1;
s3, preparing a mother solution, adding lysozyme into deionized water with the volume 15 times that of the lysozyme, adjusting the temperature of a lysozyme aqueous solution to 40 ℃ after the lysozyme is fully dissolved, and adjusting the pH value to 6.4, and finally adding the extraction liquids of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla striata obtained by purification in the step S2 into the lysozyme aqueous solution in sequence and mixing uniformly, wherein the adding speed of the extraction liquids of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla striata is 0.5m/min, and the adding time interval of two adjacent extraction liquids;
and S4, packaging, namely adding the mixed liquid obtained in the step S3 into a dark glass bottle through canning equipment, sealing, and storing in an environment which is protected from light and has the temperature of not more than 20 ℃ for later use.
In step S1, the volumes of the alcohol aqueous solutions for soaking are 10 times the volumes of the powders to be soaked respectively.
It is noted that the residues from the steps S2 and S3 are collected together, added to a fermentation device for fermentation, and the fermented mixture is used as feed material.
Example 3
A liquid feed additive for solving the intestinal problem of animals comprises the following components in percentage by mass: 18 percent of rhubarb, 6.3 percent of astragalus, 12 percent of wrinkled gianthyssop herb, 4.1 percent of rhizoma atractylodis, 8.5 percent of bletilla and the balance of lysozyme.
In the embodiment, the rhubarb, the astragalus, the wrinkled gianthyssop, the rhizoma atractylodis and the bletilla striata are all Chinese herbal pieces.
A preparation method of a liquid feed additive for solving the intestinal problem of animals comprises the following steps:
s1, extracting Chinese medicine, storing rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata respectively, cooling to-8 ℃ and keeping the temperature, then keeping the temperature for 10 minutes, then respectively carrying out ultrasonic crushing on the rhubarb, the astragalus root, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata to prepare powder with the particle size of 7 mu m, then respectively adding the powder of the rhubarb, the astragalus root, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata into alcohol aqueous solution with the temperature of 25 ℃ and the concentration of 65 percent to soak for 50 minutes to obtain mixed solution, then carrying out supercritical fluid extraction on the mixed solution firstly, then carrying out membrane extraction separation on the extracted residues, finally collecting and mixing the supercritical fluid extract liquid and the separated liquid after the membrane extraction separation, and carrying out reduced pressure concentration on the mixed solution to ensure that the volume of the concentrated solution is 80 percent of the original, extracting the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata, and collecting and treating the residues after the extraction;
s2, purifying, namely naturally cooling the extract liquor of the rhubarb, the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata obtained by the preparation in the step S1 to room temperature, adding ethanol into the extract liquor until the alcohol degree of the extract liquor reaches 60%, carrying out alcohol precipitation, taking supernate after the alcohol precipitation is finished, carrying out reduced pressure alcohol recovery on the supernate until the ethanol content in the supernate is not more than 3%, carrying out alcohol precipitation on the supernate, carrying out secondary alcohol precipitation, collecting the supernate obtained after the secondary alcohol precipitation, carrying out reduced pressure alcohol recovery for later use, carrying out solid-liquid separation, centrifugal separation and ionic membrane extraction and separation on the supernate obtained after the secondary alcohol precipitation, filtering by using a 0.22-micron microporous filter, collecting the filtered filtrate for later use, and simultaneously mixing and collecting residues obtained in the operation with the residue obtained in the step S1;
s3, preparing a mother solution, adding lysozyme into deionized water with the volume at least 5 times that of the lysozyme, adjusting the temperature of a lysozyme aqueous solution to 20 ℃ after the lysozyme is fully dissolved, and adjusting the pH value to 5.8, finally adding the extraction liquid of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla striata obtained by the purification in the step S2 into the lysozyme aqueous solution in sequence, and uniformly mixing, wherein the adding speed of the extraction liquid of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla striata is 0.3m/min, and the adding time interval of two adjacent;
and S4, packaging, namely adding the mixed liquid obtained in the step S3 into a dark glass bottle through canning equipment, sealing, and storing in an environment which is protected from light and has the temperature of not more than 20 ℃ for later use.
Meanwhile, in the step S1, the volumes of the alcohol aqueous solutions for soaking are respectively 5 times of the volumes of the powders soaked in the alcohol aqueous solutions.
Preferably, the residues in the steps S2 and S3 are collected together and then added into a fermentation device for fermentation operation, and the fermented mixture is used as feed raw materials.
Example 4
A liquid feed additive for solving the intestinal problem of animals comprises the following components in percentage by mass: rhubarb 22%, astragalus root 7.3%, wrinkled gianthyssop 11%, atractylodes rhizome 6.5%, bletilla striata 8.2%, and lysozyme in balance.
In the embodiment, the rhubarb, the astragalus, the wrinkled gianthyssop, the rhizoma atractylodis and the bletilla striata are all Chinese herbal pieces.
A preparation method of a liquid feed additive for solving the intestinal problem of animals comprises the following steps:
s1, extracting Chinese medicine, storing rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata respectively, cooling to-5 ℃ and keeping the temperature, then keeping the temperature for 15 minutes, then respectively carrying out ultrasonic crushing on the rhubarb, the astragalus root, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata to prepare powder with the particle size of 8 mu m, then respectively adding the powder of the rhubarb, the astragalus root, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata into alcohol aqueous solution with the temperature of 37 ℃ and the concentration of 70 percent to be soaked for 100 minutes to obtain mixed solution, then carrying out supercritical fluid extraction on the mixed solution firstly, then carrying out membrane extraction separation on the extracted residues, finally collecting and mixing the separated liquid after the membrane extraction separation, carrying out reduced pressure concentration on the mixed solution to ensure that the volume of the concentrated solution is 82 percent of the original volume to obtain the rhubarb, extracting the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata, and collecting and treating the residues after the extraction;
s2, purifying, namely naturally cooling the extract liquor of the rhubarb, the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata obtained by the preparation in the step S1 to room temperature, adding ethanol into the extract liquor until the alcohol degree of the extract liquor reaches 60%, carrying out alcohol precipitation, taking supernate after the alcohol precipitation is finished, carrying out reduced pressure alcohol recovery on the supernate until the ethanol content in the supernate is 1%, carrying out alcohol precipitation on the supernate, carrying out secondary alcohol precipitation, collecting the supernate obtained after the secondary alcohol precipitation, carrying out reduced pressure alcohol recovery for later use, filtering the supernate obtained after the secondary alcohol precipitation through a 0.22-micron microporous filter after solid-liquid separation, centrifugal separation and ionic membrane extraction and separation, collecting the filtered filtrate for later use, and simultaneously mixing and collecting residues obtained in the operation with residues obtained in the step S1;
s3, preparing a mother solution, adding lysozyme into deionized water with the volume 8 times that of the lysozyme, adjusting the temperature of a lysozyme aqueous solution to 32 ℃ after the lysozyme is fully dissolved, and adjusting the pH value to 6, and finally adding the extraction liquids of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata obtained by purification in the step S2 into the lysozyme aqueous solution in sequence and mixing uniformly, wherein the adding speed of the extraction liquids of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the atractylodes rhizome and the bletilla striata is 0.2m/min, and the adding time interval of two adjacent extraction liquids is 8;
and S4, packaging, namely adding the mixed liquid obtained in the step S3 into a dark glass bottle through canning equipment, sealing, and storing in an environment which is protected from light and has the temperature of not more than 20 ℃ for later use.
In this embodiment, in the step S1, the volumes of the alcohol aqueous solutions for soaking are respectively 3 to 10 times of the volumes of the powders to be soaked.
In this embodiment, the residues from the steps S2 and S3 are collected together, and then added to a fermentation device for fermentation, and the fermented mixture is used as a feed material.
In a specific use of the invention, 2ml to 3ml are taken orally at a time. Wherein:
lysozyme is an alkaline enzyme that hydrolyzes mucopolysaccharides in pathogenic bacteria. The cell wall insoluble mucopolysaccharides are broken down into soluble glycopeptides, primarily by disrupting the cell wall, resulting in the escape of the cell wall ruptured contents and lysis of the bacteria. Lysozyme can also bind directly to negatively charged viral proteins, inactivating viruses. The enzyme has antibacterial, antiinflammatory, and antiviral effects.
The traditional Chinese medicine component emodin can affect the folic acid enzyme system, induce interferon, inhibit the propagation of escherichia coli and reduce the drug resistance of bacteria; the astragalus can induce the organism to generate interferon, and plays roles of enhancing immunity and resisting virus; hexosamine in rhizoma Atractylodis can increase blood flow of intestinal mucosa tissue, and promote intestinal mucosa repair; the gallic acid in rhizoma Bletillae and radix et rhizoma Rhei can promote adhesion and aggregation of blood platelet, and can contract blood vessel of injured part to achieve hemostatic effect. The traditional Chinese medicine extract has scientific compatibility, can promote gastrointestinal motility, accelerate the discharge of toxic substances in intestinal tracts, protect gastrointestinal mucosa, inhibit the exudation of mucosal inflammatory substances, and quickly relieve enteritis and diarrhea symptoms.
On one hand, the invention has simple preparation process and wide raw material source, thereby greatly reducing the cost of raw materials and processing operation; on the other hand, the feed additive has small toxic and side effects, can effectively balance animal intestinal flora, inhibit the reproduction and growth of harmful bacteria, kill the harmful bacteria, simultaneously adjust the balance of the intestinal flora, and promote the digestion and absorption of animals to the feed, thereby effectively solving the problems of diarrhea and poor nutrient absorption of the animals caused by intestinal diseases, and simultaneously, assisting in achieving the purposes of increasing the weight and the yield of the animals, improving the breeding efficiency, and reducing the breeding cost and the risk.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (5)
1. A liquid feed additive for solving the intestinal problem of animals is characterized in that: the liquid feed additive for solving the intestinal problems of animals comprises the following components in percentage by mass: 15-25% of rheum officinale, 5-8.3% of astragalus membranaceus, 10-22% of wrinkled gianthyssop herb, 3.5-7.1% of rhizoma atractylodis, 7.2-9.5% of bletilla striata and the balance lysozyme.
2. The liquid feed additive for solving the intestinal problem of animals as claimed in claim 1, wherein: the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla are all Chinese herbal pieces.
3. A preparation method of a liquid feed additive for solving the intestinal problem of animals is characterized by comprising the following steps: the preparation method of the fermentation mixed type feed additive for preventing African swine fever comprises the following steps:
s1, extracting Chinese medicine, storing rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata, cooling to-10-0 ℃, keeping the temperature, then keeping the temperature for 5-25 minutes, then ultrasonic crushing the rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata to obtain powder with the particle size of 5-10 μm, then adding the powder of rhubarb, astragalus root, wrinkled gianthyssop herb, atractylodes rhizome and bletilla striata into alcohol water solution with the temperature of 20-45 ℃ and the concentration of 60-75% to soak for 30-120 minutes to obtain mixed liquid, then performing supercritical fluid extraction on the mixed liquid, performing membrane extraction and separation on the extracted residues, finally collecting and mixing the supercritical fluid extract and the separated liquid after membrane extraction, performing reduced pressure concentration on the mixed liquid to make the volume of the concentrated liquid 70-90% of the original volume, thus obtaining the extract liquid of rhubarb, astragalus, wrinkled gianthyssop herb, rhizoma atractylodis and bletilla striata, and collecting and processing the residue after removal;
s2, purifying, namely naturally cooling the extract liquor of the rhubarb, the astragalus, the agastache rugosus, the rhizoma atractylodis and the bletilla striata obtained by the preparation in the step S1 to room temperature, adding ethanol into the extract liquor until the alcohol degree of the extract liquor reaches 60%, carrying out alcohol precipitation, taking supernate after the alcohol precipitation is finished, carrying out reduced pressure alcohol recovery on the supernate until the ethanol content in the supernate is not more than 3%, carrying out alcohol precipitation on the supernate, carrying out secondary alcohol precipitation, collecting the supernate obtained after the secondary alcohol precipitation, carrying out reduced pressure alcohol recovery for later use, carrying out solid-liquid separation, centrifugal separation and ionic membrane extraction and separation on the supernate obtained after the secondary alcohol precipitation, filtering by using a 0.22-micron microporous filter, collecting the filtered filtrate for later use, and simultaneously mixing and collecting residues obtained in the operation with the residue obtained in the step S1;
s3, preparing a mother solution, adding lysozyme into deionized water with the volume at least 5 times that of the lysozyme, adjusting the temperature of a lysozyme aqueous solution to 15-40 ℃ after the lysozyme is fully dissolved, and adjusting the pH value to 5.3-6.4, and finally adding the extraction liquid of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla obtained by purification in the step S2 into the lysozyme aqueous solution in sequence and mixing uniformly, wherein the adding speed of the extraction liquid of the rhubarb, the astragalus, the wrinkled gianthyssop herb, the rhizoma atractylodis and the bletilla is 0.1-0.5 m/min, and the adding time interval of two adjacent extraction liquid is 3-15 minutes;
and S4, packaging, namely adding the mixed liquid obtained in the step S3 into a dark glass bottle through canning equipment, sealing, and storing in an environment which is protected from light and has the temperature of not more than 20 ℃ for later use.
4. The liquid feed additive for solving the intestinal problem of animals as claimed in claim 1, wherein: in the step S1, the volumes of the alcohol aqueous solutions for soaking are respectively 3 to 10 times of the volumes of the powders soaked in the alcohol aqueous solutions.
5. The liquid feed additive for solving the intestinal problem of animals as claimed in claim 1, wherein: and (3) after the residues in the steps S2 and S3 are collected together, adding the collected residues into fermentation equipment for fermentation operation, and utilizing the fermented mixture as a feed raw material.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112640886A (en) * | 2020-12-18 | 2021-04-13 | 洛阳市洛瑞牧业有限公司 | Cattle frozen semen diluent and preparation method thereof |
| CN115462460A (en) * | 2022-09-19 | 2022-12-13 | 河南省科学院生物研究所有限责任公司 | Preparation method and application of liquid bacterial enzyme synergistic feed additive for pigs |
-
2020
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112640886A (en) * | 2020-12-18 | 2021-04-13 | 洛阳市洛瑞牧业有限公司 | Cattle frozen semen diluent and preparation method thereof |
| CN115462460A (en) * | 2022-09-19 | 2022-12-13 | 河南省科学院生物研究所有限责任公司 | Preparation method and application of liquid bacterial enzyme synergistic feed additive for pigs |
| CN115462460B (en) * | 2022-09-19 | 2023-10-24 | 河南省科学院生物研究所有限责任公司 | Preparation method and application of liquid bacterial enzyme synergistic feed additive for pigs |
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