CN112210834B - 利用单管添加方案的加标签的核酸的文库制备 - Google Patents
利用单管添加方案的加标签的核酸的文库制备 Download PDFInfo
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- CN112210834B CN112210834B CN202010451081.2A CN202010451081A CN112210834B CN 112210834 B CN112210834 B CN 112210834B CN 202010451081 A CN202010451081 A CN 202010451081A CN 112210834 B CN112210834 B CN 112210834B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Genetics & Genomics (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462017786P | 2014-06-26 | 2014-06-26 | |
| US62/017,786 | 2014-06-26 | ||
| US201462027198P | 2014-07-21 | 2014-07-21 | |
| US62/027,198 | 2014-07-21 | ||
| CN201580046283.6A CN106795651B (zh) | 2014-06-26 | 2015-06-25 | 利用单管添加方案的加标签的核酸的文库制备 |
| PCT/US2015/037653 WO2015200609A1 (en) | 2014-06-26 | 2015-06-25 | Library preparation of tagged nucleic acid using single tube add-on protocol |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580046283.6A Division CN106795651B (zh) | 2014-06-26 | 2015-06-25 | 利用单管添加方案的加标签的核酸的文库制备 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112210834A CN112210834A (zh) | 2021-01-12 |
| CN112210834B true CN112210834B (zh) | 2025-02-25 |
Family
ID=53761498
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010451081.2A Active CN112210834B (zh) | 2014-06-26 | 2015-06-25 | 利用单管添加方案的加标签的核酸的文库制备 |
| CN201580046283.6A Active CN106795651B (zh) | 2014-06-26 | 2015-06-25 | 利用单管添加方案的加标签的核酸的文库制备 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580046283.6A Active CN106795651B (zh) | 2014-06-26 | 2015-06-25 | 利用单管添加方案的加标签的核酸的文库制备 |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US10017759B2 (OSRAM) |
| EP (2) | EP3754020B1 (OSRAM) |
| JP (1) | JP6626848B2 (OSRAM) |
| CN (2) | CN112210834B (OSRAM) |
| AU (1) | AU2015279862B2 (OSRAM) |
| CA (1) | CA2953367A1 (OSRAM) |
| DK (1) | DK3161137T3 (OSRAM) |
| ES (2) | ES2811518T3 (OSRAM) |
| WO (1) | WO2015200609A1 (OSRAM) |
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| PL2814959T3 (pl) | 2012-02-17 | 2018-07-31 | Fred Hutchinson Cancer Research Center | Kompozycje i sposoby do dokładnej identyfikacji mutacji |
| WO2014013259A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
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