CN112169849A - Micro-fluidic chip for simultaneously separating and capturing wide-scale magnetic materials - Google Patents
Micro-fluidic chip for simultaneously separating and capturing wide-scale magnetic materials Download PDFInfo
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- CN112169849A CN112169849A CN201911295589.1A CN201911295589A CN112169849A CN 112169849 A CN112169849 A CN 112169849A CN 201911295589 A CN201911295589 A CN 201911295589A CN 112169849 A CN112169849 A CN 112169849A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
Abstract
The invention discloses a micro-fluidic chip for simultaneously separating and capturing wide-scale magnetic materials, wherein a capturing cavity, an injection port and a discharge port are arranged in the micro-fluidic chip, the injection port and the discharge port are respectively communicated with two ends of the capturing cavity, the capturing cavity is concave step-shaped, the height of the capturing cavity is gradually reduced from the injection port to the discharge port, the capturing cavity is composed of a micron-scale capturing area and a nano-scale capturing area, the nano-scale capturing area is provided with a magnetic capturing area, the magnetic capturing area is in an expanded shape, and a magnetic structure is arranged in the magnetic capturing area. The micro-fluidic chip has the advantages of simple structure, convenient manufacture, low cost and easy realization of simultaneous separation and capture of magnetic materials from micron to nanometer.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a micro-fluidic chip for separating and capturing wide-scale magnetic materials simultaneously.
Background
The micro-fluidic chip can integrate basic operation units related to sample preparation, reaction, separation, detection and the like in the fields of biology, chemistry, medicine and the like on a chip with the size of a few square centimeters or even smaller, has the characteristics of miniaturization, automation, integration and the like, and is widely applied to the field of biomedicine. Due to the fact that manipulation means such as optical, electric, sound, magnetism, size, fluid and temperature can be conveniently integrated in the microfluidic chip, the micro-fluidic chip serves research objects. Compared with other modes, the magnetic and size control mode has the characteristics of simplicity in operation, easiness in implementation and the like, the surface of the magnetic material is convenient to modify, the influence of the environment is small, and the magnetic material is widely applied to the field of biomedicine. However, at present, magnetic materials with different dimensions are difficult to be separated simply and efficiently through the manipulation of a magnetic field because the magnetic force of the magnetic materials is not very different.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the micro-fluidic chip for separating and capturing the wide-scale magnetic materials simultaneously, which has the advantages of simple structure, convenience in manufacturing, low cost and easiness in realizing the simultaneous separation and capture of the micro-scale to nano-scale magnetic materials.
The technical scheme adopted for realizing the above purpose of the invention is as follows:
a micro-fluidic chip for simultaneously separating and capturing wide-scale magnetic materials is provided with a capturing cavity, an injection port and a discharge port, wherein the injection port and the discharge port are respectively communicated with two ends of the capturing cavity, the capturing cavity is concave step-shaped, the height of the capturing cavity is gradually reduced from the injection port to the discharge port, the capturing cavity is composed of a micron-scale capturing area and a nanometer-scale capturing area, the nanometer-scale capturing area is provided with a magnetic capturing area, the magnetic capturing area is in an expanded shape, and a magnetic structure is arranged in the magnetic capturing area.
The microfluidic chip is composed of a glass cover plate and a conductive substrate, wherein a fluid channel is arranged on one surface of the glass cover plate, an injection port and a discharge port are respectively arranged on the glass cover plate, the injection port and the discharge port are respectively communicated with two ends of the fluid channel, the fluid channel is in a concave step shape, the depth of the fluid channel is gradually reduced from the injection port to the discharge port, an expansion area is arranged on one side, close to the discharge port, of the fluid channel, the surface of the fluid channel, arranged on the glass cover plate, is in sealed connection with the conductive surface of the conductive substrate, a cavity formed by the fluid channel on the glass cover plate and the conductive substrate in a sealed mode is a capture cavity, a cavity formed by the expansion area on the glass cover plate and the conductive substrate in a sealed mode.
The magnetic structure comprises an array of magnetically permeable material.
The magnetic conductive material is a nickel block, and the magnetic structure is a nickel matrix formed by the nickel block.
The nickel block is square, the height of the magnetic capture area is 2-50 μm, and the heights of the nickel matrix and the nickel block are 1/2 no greater than the height of the magnetic capture area.
The projection of the fluid channel on the glass cover plate is in a square strip shape.
The capture cavity is in a two-stage concave step shape, the part with the larger height of the capture cavity is a micron-scale capture area, and the part with the smaller height of the capture cavity is a nano-scale capture area.
Compared with the prior art, the invention has the beneficial effects and advantages that:
1. the micro-fluidic chip combines size capture and magnetic capture by arranging the recessed step-shaped capture cavity and the magnetic capture area with the magnetic structure, realizes the simultaneous separation and capture of magnetic materials with the same magnetic force but different sizes, is easy to realize the simultaneous separation and capture of magnetic materials from micron to nanometer, and can carry out more sufficient biochemical reaction due to the excellent dynamics of the magnetic materials from nanometer.
2. The liquid sample is injected from the injection port of the microfluidic chip, and the column pressure in the separation process can be effectively avoided through the recessed step-shaped fluid channel.
3. The microfluidic chip performs magnetic separation and enrichment on a liquid sample, is small in external interference factor, and can conveniently pass through the lowest region of the depressed area due to the nanoscale magnetic material, so that a fluid channel is not easily blocked.
4. The micro-fluidic chip has the advantages of simple structure, easy manufacture and processing, low manufacture cost, short capture time and high capture efficiency.
Drawings
Fig. 1 is a schematic structural diagram of a microfluidic chip for separating and capturing wide-scale magnetic materials simultaneously.
Fig. 2 is a partial enlarged view of I in fig. 1.
Fig. 3 is a partially enlarged view of J in fig. 1.
Fig. 4 is a cross-sectional view of fig. 1.
Wherein, 1-glass cover plate, 2-conductive substrate, 3-injection port, 4-discharge port, 5-capture cavity, 6-micron capture zone, 7-nanometer capture zone, 8-magnetic capture zone, 9-nickel matrix, 10-nickel block, and 11-fluid channel.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments.
The structure of the microfluidic chip for separating and capturing wide-scale magnetic materials provided by the invention is shown in fig. 1 and 4, and comprises a glass cover plate 1 and a conductive substrate 2.
The material of the cover glass 1 is common optical glass. The glass cover plate is provided with a fluid channel 11, an injection port 3 and a discharge port 4, and the injection port 3 and the discharge port 4 are respectively communicated with two ends of the fluid channel. The fluid channel is in a secondary concave step shape, and the projection of the fluid channel on the glass cover plate is in a square strip shape. In this embodiment, the total length of the fluid channel is 50mm, and the width thereof is 3 mm. The end with larger depth of the fluid channel is communicated with the injection port 3, the end with smaller depth of the fluid channel is communicated with the discharge port 4, the part with smaller depth of the fluid channel is provided with an expanded area which is in a cubic shape, and the depth of the expanded area is 50 μm.
The conductive substrate 2 is made of ITO conductive glass, a nickel matrix 9 formed by nickel blocks is plated on an indium tin oxide film of the conductive substrate, and rows of the nickel matrix 9 are perpendicular to the length direction of the fluid channel. The nickel block 10 is square, the height of the nickel block 10 is 1/2 of the depth of the expansion zone, and in the embodiment, the side length of the nickel block is 25 μm.
The glass cover plate 1 and the conductive glass 2 are connected in a sealing mode through bonding, the surface of the glass cover plate 1, which is provided with the fluid channel, is attached to the surface of the conductive substrate 2, which is provided with the indium tin oxide film, the fluid channel 11 on the glass cover plate 1 and the conductive substrate 2 are sealed to form a cavity body which is a capturing cavity 5, and the expansion area on the glass cover plate 1 and the cavity body formed by the conductive substrate 2 in a sealing mode are magnetic capturing areas 8. The capture cavity 5 is in a two-stage concave step shape, the capture cavity 5 is composed of a micron-scale capture area 6 and a nanometer-scale capture area 7, the part with the larger height of the capture cavity 5 is the micron-scale capture area 6, the part with the smaller height of the capture cavity 5 is the micron-scale capture area 7, and the nickel matrix 10 is positioned in the magnetic capture area 9. In this embodiment, the micro-scale trapping region has a height of 50 μm, a length of 20mm, and a width of 3mm, the nano-scale trapping region has a height of 2 μm, a length of 30mm, and a width of 3mm, and the magnetic trapping region has a height of 50 μm, a length of 5mm, and a width of 3 mm.
First, the invention discloses a sorting experiment of a microfluidic chip for separating and capturing wide-scale magnetic materials simultaneously
The test method comprises the following steps:
1. two magnets are fixed right below a magnetic capture area of the microfluidic chip and then are positioned in the middle of the lower part of the nickel matrix;
2. mixing 20mL of Affimag SLE magnetic microspheres with the diameter of 10 mu m and 20mL of Affimag SLE magnetic microspheres with the diameter of 380nm to obtain magnetic materials with mixed sizes;
3. adding 160mL of DI water into the magnetic material to obtain 200mL of magnetic material mixed solution;
4. 200mL of the mixed liquid of the magnetic material was injected into the fluid channel from the injection port at a rate of 10. mu.L/min, and observed with a microscope.
The experimental results are as follows:
magnetic microspheres with the diameter of 10 mu m are enriched in the micron-sized capture zone, and magnetic microspheres with the diameter of 380nm are enriched in the magnetic capture zone.
Claims (7)
1. A wide-scale magnetic material simultaneous separation and capture microfluidic chip is characterized in that: the micro-fluidic chip is provided with a capture cavity, an injection port and a discharge port, the injection port and the discharge port are respectively communicated with two ends of the capture cavity, the capture cavity is in a concave step shape, the height of the capture cavity is gradually reduced from the injection port to the discharge port, the capture cavity is composed of a micron-scale capture area and a nano-scale capture area, the nano-scale capture area is provided with a magnetic capture area, the magnetic capture area is in an expanded shape, and a magnetic structure is arranged in the magnetic capture area.
2. The microfluidic chip for simultaneous separation and capture of wide-scale magnetic materials according to claim 1, wherein: the microfluidic chip is composed of a glass cover plate and a conductive substrate, wherein a fluid channel is arranged on one surface of the glass cover plate, an injection port and a discharge port are respectively arranged on the glass cover plate, the injection port and the discharge port are respectively communicated with two ends of the fluid channel, the fluid channel is in a concave step shape, the depth of the fluid channel is gradually reduced from the injection port to the discharge port, an expansion area is arranged on one side, close to the discharge port, of the fluid channel, the surface of the fluid channel, arranged on the glass cover plate, is in sealed connection with the conductive surface of the conductive substrate, a cavity formed by the fluid channel on the glass cover plate and the conductive substrate in a sealed mode is a capture cavity, a cavity formed by the expansion area on the glass cover plate and the conductive substrate in a sealed mode.
3. The microfluidic chip for simultaneous separation and capture of wide-scale magnetic materials according to claim 2, wherein: the magnetic structure comprises an array of magnetically permeable material.
4. The microfluidic chip for simultaneous separation and capture of wide-scale magnetic materials according to claim 3, wherein: the magnetic conductive material is a nickel block, and the magnetic structure is a nickel matrix formed by the nickel block.
5. The microfluidic chip for simultaneous separation and capture of wide-scale magnetic materials according to claim 4, wherein: the nickel block is square, the height of the magnetic capture area is 2-50 μm, and the heights of the nickel matrix and the nickel block are 1/2 no greater than the height of the magnetic capture area.
6. The microfluidic chip for simultaneous separation and capture of wide-scale magnetic materials according to claim 2, wherein: the projection of the fluid channel on the glass cover plate is in a square strip shape.
7. The microfluidic chip for simultaneous separation and capture of wide-scale magnetic materials according to claim 1, wherein: the capture cavity is in a two-stage concave step shape, the part with the larger height of the capture cavity is a micron-scale capture area, and the part with the smaller height of the capture cavity is a nano-scale capture area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911295589.1A CN112169849A (en) | 2019-12-16 | 2019-12-16 | Micro-fluidic chip for simultaneously separating and capturing wide-scale magnetic materials |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911295589.1A CN112169849A (en) | 2019-12-16 | 2019-12-16 | Micro-fluidic chip for simultaneously separating and capturing wide-scale magnetic materials |
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| CN112169849A true CN112169849A (en) | 2021-01-05 |
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| CN201911295589.1A Pending CN112169849A (en) | 2019-12-16 | 2019-12-16 | Micro-fluidic chip for simultaneously separating and capturing wide-scale magnetic materials |
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Citations (6)
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| US20070264649A1 (en) * | 2004-10-15 | 2007-11-15 | Walter Gumbrecht | Method for the Combined Isolation of Magnet-Beads From a Liquid Sample and Subsequent Thermocyclisation for the Polymerase Chain Reaction (Pcr) and Associated Arrangement |
| CN105457690A (en) * | 2015-12-23 | 2016-04-06 | 武汉纺织大学 | Micro-fluidic chip of stepped structure and preparing method thereof |
| US20170328893A1 (en) * | 2014-11-28 | 2017-11-16 | Indian Institute Of Technology Delhi | Magnetic capturing of rare cells |
| CN109550531A (en) * | 2019-01-28 | 2019-04-02 | 武汉纺织大学 | A kind of micro-fluidic chip that magnetism size relies on |
| CN109746064A (en) * | 2019-01-28 | 2019-05-14 | 武汉纺织大学 | A kind of gradient magnetic micro-fluidic chip |
| CN110108623A (en) * | 2019-04-30 | 2019-08-09 | 武汉纺织大学 | A kind of greasy dirt grain testing apparatus and method based on micro-fluidic chip |
-
2019
- 2019-12-16 CN CN201911295589.1A patent/CN112169849A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070264649A1 (en) * | 2004-10-15 | 2007-11-15 | Walter Gumbrecht | Method for the Combined Isolation of Magnet-Beads From a Liquid Sample and Subsequent Thermocyclisation for the Polymerase Chain Reaction (Pcr) and Associated Arrangement |
| US20170328893A1 (en) * | 2014-11-28 | 2017-11-16 | Indian Institute Of Technology Delhi | Magnetic capturing of rare cells |
| CN105457690A (en) * | 2015-12-23 | 2016-04-06 | 武汉纺织大学 | Micro-fluidic chip of stepped structure and preparing method thereof |
| CN109550531A (en) * | 2019-01-28 | 2019-04-02 | 武汉纺织大学 | A kind of micro-fluidic chip that magnetism size relies on |
| CN109746064A (en) * | 2019-01-28 | 2019-05-14 | 武汉纺织大学 | A kind of gradient magnetic micro-fluidic chip |
| CN110108623A (en) * | 2019-04-30 | 2019-08-09 | 武汉纺织大学 | A kind of greasy dirt grain testing apparatus and method based on micro-fluidic chip |
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