CN211896990U - Micron particle arrangement device - Google Patents
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- CN211896990U CN211896990U CN202020044435.7U CN202020044435U CN211896990U CN 211896990 U CN211896990 U CN 211896990U CN 202020044435 U CN202020044435 U CN 202020044435U CN 211896990 U CN211896990 U CN 211896990U
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- 239000002245 particle Substances 0.000 title claims abstract description 78
- 238000004720 dielectrophoresis Methods 0.000 claims abstract description 15
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- 239000011859 microparticle Substances 0.000 claims description 23
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- 239000002184 metal Substances 0.000 claims description 2
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- 239000008187 granular material Substances 0.000 abstract description 7
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- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 8
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 8
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- 238000010586 diagram Methods 0.000 description 4
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- 239000012528 membrane Substances 0.000 description 4
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Abstract
The utility model belongs to micron level chip field specifically discloses a micron granule collating unit, and its permutation method has related to dielectrophoresis power. The micron particle arrangement device specifically comprises a first positive voltage electrode, a second positive voltage electrode, a first negative voltage electrode, a second negative voltage electrode, a first open port, a second open port and a straight row channel. The utility model discloses micron granule collating unit's working process is: the particles to be arranged in disorder pass through the straight-row channels after entering from the first left open port, are arranged under the action of dielectrophoresis force in the straight-row channels, and flow out of the micron particle arrangement device through the second right open port after the particles are arranged, so that the purpose of the scheme is achieved. The utility model has the advantages that: rapidly arranging the particles at a micrometer scale; the device has simple structure and high universality, and can realize the arrangement of particles under the condition of no contact external force damage.
Description
Technical Field
The utility model belongs to micron level chip field specifically discloses a micron particle collating unit, and it has related to and has operated the granule in the method of utilizing dielectrophoresis in interchange and direct current electric field to realize micron particle arrangement purpose. The invention can be used in micron-scale particle alignment processes.
Background
With scientific progress and more serious resource shortage, scientific and technical research enters the micron-level field, while the microfluidic technology is just a 'control hand' of micron-level experimental research, basic operations such as preparation, observation, separation, reaction and the like of experimental samples related in the fields of biology, chemistry and the like are carried out on a microfluidic chip, an operation unit is integrated on a chip with the size of a few square centimeters or less, and a micron-level experimental cavity is formed through a microchannel. The technology has been developed into a research field which crosses multiple disciplines such as machinery, chemistry, biology, medicine, hydromechanics and the like and has wide application prospect.
The existing particle control is mainly in a contact type or a non-contact type, the contact type mainly controls particles to reach an appointed position through a physical contact method, so that the experimental requirement is met, and the obvious defect of physical contact is that the particle surface is damaged; the non-contact type mainly uses pressure to drive liquid, the liquid drives the particles to operate and control, but the method of driving the particles by using the pressure to drive the liquid to drive the particles to operate and control the particles has low position precision and can not perform better operation and control on single particles. Meanwhile, the particles cannot be arranged under the continuous condition, and only a certain amount of particles are fed into the experiment cavity once to be arranged and then are taken out to be arranged next time, so that the experiment steps are complicated and the operation is difficult.
The invention controls the particles by a dielectrophoresis technology, the particles generate induction electric dipole moment in a non-uniform electric field, thereby generating dielectrophoresis force among the particles, and the particles are arranged by the action of the dielectrophoresis force. The invention has the advantage that the structure is designed to be an optimal structure through the repeated test optimization of the structure.
Disclosure of Invention
The invention provides a micron particle arranging device, aiming at solving the problems that the existing micron particle arranging device is complicated in steps, causes damage to particles to be arranged and is difficult to operate in batch.
An object of the utility model is to provide a micron granule collating unit, unordered the granule of waiting to arrange passes through in-line row passageway 7 after 5 the first open ports on the left side get into, receives the effect of dielectrophoresis power in-line row passageway 7 and carries out the granule and arrange, arranges through the 6 micron granule collating units that flow of the second open ports on the right after accomplishing to realize this scheme purpose.
The technical scheme of the utility model is that: a microparticle alignment device comprises a first positive voltage electrode 1, a second positive voltage electrode 2, a first negative voltage electrode 3, a second negative voltage electrode 4, a first open port 5, a second open port 6, and a straight row channel 7.
The straight arrangement channel 7 is communicated with conductive current-carrying liquid, so that the interior of the arrangement device is conductive, the current-carrying liquid simultaneously provides the effect of carrying particles, and the left side of the straight arrangement channel 7 is provided with a first open port 5, and the right side of the straight arrangement channel 7 is provided with a second open port 6. After the alternating current power supply and the direct current power supply are switched on, particles to be arranged enter the straight-row channel 7 from the first opening port 5 through the transportation of carrier fluid, the particles are finally arranged into a row in the straight-row channel 7 under the action of dielectrophoresis force and then flow out through the second opening port 6, and the purpose of particle arrangement is achieved.
The first open port 5 is electrically conductive at the position of the first open port 5 by embedding a film electrode on the wall surface, and the film electrode of the first open port 5 is connected with alternating voltage so that the film electrode of the first open port 5 is provided with alternating voltage. The second opening port 6 is electrically conducted at the position of the second opening port 6 by embedding a film electrode on the wall surface, and the film electrode of the second opening port 6 is connected with alternating voltage to enable the film electrode of the second opening port 6 to be provided with alternating voltage. The membrane electrode on the first open port 5, the carrier fluid in the in-line channel 7 and the membrane electrode on the second open port 6 form an alternating current closed loop.
The first positive voltage electrode 1 is applied to the inner wall of the in-line channel 7 in a manner of embedding a thin film electrode in the wall and is connected with a direct current positive electrode, the first negative voltage electrode 3 is applied to the inner wall of the in-line channel 7 in a manner of embedding a thin film electrode in the wall and is connected with a direct current negative electrode, and a direct current closed loop is formed by the first positive voltage electrode 1, the carrier fluid in the in-line channel 7 and the first negative voltage electrode 3.
The second positive voltage electrode 2 is applied on the inner wall of the in-line channel 7 by embedding a thin film electrode in the wall, and is connected with a direct current positive electrode. The second negative voltage electrode 4 is applied on the inner wall of the in-line channel 7 in a manner of embedding a thin film electrode in the wall, and is connected with a direct current negative electrode, and a direct current closed loop is formed by the second positive voltage electrode 2, the carrier fluid in the in-line channel 7 and the second negative voltage electrode 3.
Further, the first open port 5 has an inlet pressure, which pushes the carrier fluid to move the particles into the in-line channel 7.
Preferably, the material of the thin film electrode is ITO.
Preferably, the thin film electrode is made of metal.
In the micrometer particle arranging device, a vertical rectangular structure with a length of 500 μm and a width of 125 μm is formed in a straight arranging channel 7, thin film electrodes are embedded in the upper wall surface and the lower wall surface of the straight arranging channel 7, and thin film electrodes are embedded in the wall surfaces of a first opening port 5 and a second opening port 6. The thin film electrodes at the first open port 5 and the second open port 6 are connected with an alternating voltage; the first positive voltage electrode 1 and the second positive voltage electrode 2 are connected with the positive electrode of the direct current power supply; the first negative voltage electrode 3 and the second negative voltage electrode 4 are connected with the negative electrode of a direct current power supply. After passing through the straight alignment channel 7, the particles to be aligned are aligned and flow out of the microparticle alignment device through the right second open port 6.
The technical scheme of the utility model is that: a microparticle alignment device comprises a first positive voltage electrode 1, a second positive voltage electrode 2, a first negative voltage electrode 3, a second negative voltage electrode 4, a first open port 5, a second open port 6, and a straight row channel 7; the first positive voltage electrode 1 is connected with an external power supply to enable the first positive voltage electrode to provide 2V voltage, the first negative voltage electrode 3 is grounded and is conducted through the carrier fluid in the straight row channels 7, and the voltage provided by the first positive voltage electrode 1, the carrier fluid in the straight row channels 7 which is equivalent to a lead and the grounded first negative voltage electrode 3 form a direct current closed loop. The first positive voltage electrode 1 is applied on the inner wall of the in-line channel 7 by embedding a thin film electrode in the wall, the specific position is that the horizontal linear distance between the left end point of the first positive voltage electrode 1 and the boundary of the first open port 5 is 100 μm, and the length of the first positive voltage electrode 1 is 50 μm. The first negative voltage electrode 3 is applied on the inner wall of the in-line channel 7 by embedding a thin film electrode in the wall, specifically, the horizontal linear distance between the left end of the first negative voltage electrode 3 and the boundary of the first open port 5 is 100 μm, and the length of the first negative voltage electrode 1 is 50 μm.
The second positive voltage electrode 2 is connected with an external power supply to enable the second positive voltage electrode to provide 2V voltage, the second negative voltage electrode 4 is grounded and is conducted through the carrier fluid in the straight row channels 7, and the voltage provided by the second positive voltage electrode 2, the carrier fluid in the straight row channels 7 which is equivalent to a lead and the grounded second negative voltage electrode 4 form a direct current closed loop. The second positive voltage electrode 2 is applied on the inner wall of the in-line channel 7 by embedding a thin film electrode in the wall, the specific position is that the distance between the right end point of the second positive voltage electrode 2 and the boundary of the second open port 6 is 150 μm in a horizontal straight line, and the length of the second positive voltage electrode 1 is 50 μm. The second negative voltage electrode 4 is applied on the inner wall of the in-line channel 7 by embedding a thin film electrode in the wall, specifically, the position is that the distance between the right end of the second negative voltage electrode 4 and the boundary of the second open port 6 is 150 μm, and the length of the second negative voltage electrode 1 is 50 μm.
The first open port 5 is made conductive by embedding a thin film electrode having a width equal to that of the in-line channel 7 and a thickness of 2 μm at the first open port 5. The membrane electrode of the first open port 5 is connected with an external alternating current power supply, so that the alternating current voltage of 80V is applied to the first open port 5. The second open port 6 is made conductive by embedding a thin film electrode having a width equal to that of the in-line channel 7 and a thickness of 2 μm at the second open port 6. The membrane electrode of the second open port 6 is connected with an external alternating current power supply, so that an alternating current voltage is carried at the second open port 6, and a closed alternating current loop is formed by the first open port 5 and the second open port 6 through a current-carrying fluid of a straight row channel 7 which is equivalent to a wire action.
The structure of the micron particle arrangement device is a three-dimensional rectangular structure with a rectangular structure of 500 microns in length and 125 microns in width, and the geometric structures of the micron particle arrangement device are all made of PDMS materials. The density of the carrier fluid in the three-dimensional rectangular structure is 1000kg/m 3; the relative dielectric constant is 80F/m; the dynamic viscosity was 0.001 Pa.s. When the device works, the carrier fluid is driven by 5Pa pressure from the first opening port 5 to enable particles to enter the in-line channel 7, the dielectrophoresis force is generated in the in-line channel 7 after the micron particle arrangement device is switched on with an alternating current and direct current working power supply, disordered particles are arranged under the action of the dielectrophoresis force, and finally the arranged particles flow out of the micron particle arrangement device through the second opening port 6 to achieve the purpose of particle arrangement.
The benefits of the invention are: compared with other particle control and arrangement devices, the method for realizing the micron particle arrangement is a dielectrophoresis force technology, and the particles can be arranged without contact, external force and damage. The low direct current voltage and the low alternating current voltage can be realized by applying the left alternating current voltage and the right alternating current voltage and the upper direct current voltage and the lower direct current voltage, the damage to micron-sized biological cell particles is less, and the method is suitable for detection, reaction and sample preparation in the fields of biology, medicine and the like. According to the invention, after the particles to be arranged enter through the pressure-driven carrier fluid, the arrangement purpose can be realized only by applying an electric field.
Drawings
FIG. 1 is a schematic two-dimensional view of the whole structure of the microparticle arrangement device.
FIG. 2(A) is a graph showing the time displacement of particles at t1 in the operation of the microparticle arrangement device.
FIG. 2(B) is a graph showing the time displacement of particles at t2 in the operation of the microparticle arrangement device.
FIG. 2(C) is a graph showing the time displacement of particles at t3 in the operation of the microparticle arrangement device.
FIG. 2(D) is a graph showing the time displacement of the particles at t4 in the operation of the microparticle arrangement device.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings, but the embodiments of the present invention are not limited thereto.
In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
A micron particle arrangement device is characterized in that PDMS is used as a material, a SUB mold is manufactured through manufacturing processes such as photoetching and developing, PDMS materials are mixed with curing agents and then coated on the mold, a PDMS negative film is obtained through heating, cooling and shaping and then demolding, then the PDMS materials mixed with the curing agents are coated on a PC sheet, a PDMS flat plate is obtained through heating and solidification and demolding, a thin plate electrode is embedded on the flat plate, and a microstructure obtained through matching the PDMS negative film and the flat plate is the micron particle arrangement device.
Specifically, as shown in fig. 1, a microparticle alignment apparatus. Comprises a first positive voltage electrode 1, a second positive voltage electrode 2, a first negative voltage electrode 3, a second negative voltage electrode 4, a first open port 5, a second open port 6 and a straight row channel 7. The micrometer particle arrangement device is a rectangular structure, a three-dimensional rectangular structure with the length of 500 micrometers and the width of 125 micrometers, and the geometric structures of the micrometer particle arrangement device are made of PDMS materials. The density of the carrier fluid in the three-dimensional rectangular structure is 1000kg/m 3; the relative dielectric constant is 80F/m; the dynamic viscosity was 0.001 Pa.s. When the device works, the carrier fluid is driven by 5Pa pressure to carry particles to be arrayed into the in-line channel 7 from the first open port 5, the micro-particle arraying device generates dielectrophoresis force in the in-line channel 7 after being connected with a working power supply with 80V alternating current voltage and 2V direct current voltage, disordered particles are arrayed under the action of the dielectrophoresis force, and finally the arrayed particles flow out of the micro-particle arraying device through the second open port 6 to realize the purpose of particle arraying.
Specifically, as shown in fig. 2(a), the displacement diagram of the particles to be aligned at time t1 during the operation of the microparticle aligning device is shown. Solid open circles in the figure characterize microparticles. The carrier fluid is driven by pressure to carry the particles to be arranged into the in-line arrangement channel, and the micron particles are arranged under the action of dielectrophoresis force.
Specifically, as shown in fig. 2(B), the displacement diagram of the particles to be aligned at time t2 during the operation of the microparticle aligning device is shown. The open circles are realized in the figure to characterize the microparticles. The pressure drives the carrier fluid to carry the particles to be aligned to flow to the second open port, and the particles in the carrier fluid move along with the carrier fluid. The microparticles are aligned under the action of dielectrophoretic force.
Specifically, as shown in fig. 2(C), the displacement diagram of the particles to be aligned at time t3 during the operation of the microparticle aligning device is shown. The open circles are realized in the figure to characterize the microparticles. The pressure drives the carrier fluid to carry the particles to be aligned to flow to the second open port, and the particles in the carrier fluid move along with the carrier fluid. The microparticles are aligned under the action of dielectrophoretic force.
Specifically, as shown in fig. 2(D), the displacement diagram of the particles to be aligned at time t4 during the operation of the microparticle aligning device is shown. The open circles are realized in the figure to characterize the microparticles. The pressure-driven carrier fluid carries the particles to be aligned and transports the aligned particles out of the microparticle alignment device through the second open port.
The invention is not to be considered as being limited to the details shown, since various modifications and equivalent arrangements may be made without departing from the spirit and scope of the invention.
Claims (4)
1. A microparticle alignment apparatus, comprising: the device specifically comprises a first positive voltage electrode (1), a second positive voltage electrode (2), a first negative voltage electrode (3), a second negative voltage electrode (4), a first open port (5), a second open port (6) and a straight row channel (7);
the conductive carrier fluid is filled in the straight-row channels (7) to enable the interior of the arranging device to be conductive, the left sides of the straight-row channels (7) are provided with first open ports (5), the right sides of the straight-row channels (7) are provided with second open ports (6), particles to be arranged enter the straight-row channels (7) from the first open ports (5) through the transportation of the carrier fluid, and the particles are finally arranged into a row in the straight-row channels (7) under the action of dielectrophoresis force and then flow out through the second open ports (6);
the first open port (5) is electrically conducted at the first open port (5) by embedding a film electrode on the wall surface, alternating voltage is connected at the film electrode of the first open port (5), so that alternating voltage is applied at the film electrode of the first open port (5), the second open port (6) is electrically conducted at the second open port (6) by embedding a film electrode on the wall surface, the alternating voltage is connected at the film electrode of the second open port (6), so that alternating voltage is applied at the film electrode of the second open port (6), and the film electrode on the first open port (5), the current-carrying liquid in the direct row channel (7) and the film electrode on the second open port (6) form an alternating current closed loop;
the first positive voltage electrode (1) is applied to the inner wall of the in-line channel (7) in a manner of embedding a film electrode in the wall and is connected with a direct current positive electrode, the first negative voltage electrode (3) is applied to the inner wall of the in-line channel (7) in a manner of embedding a film electrode in the wall and is connected with a direct current negative electrode, and a direct current closed loop is formed by the first positive voltage electrode (1), the current-carrying liquid in the in-line channel (7) and the first negative voltage electrode (3);
the second positive voltage electrode (2) is applied to the inner wall of the in-line channel (7) in a manner of embedding a film electrode in the wall and is connected with a direct current positive electrode, the second negative voltage electrode (4) is applied to the inner wall of the in-line channel (7) in a manner of embedding a film electrode in the wall and is connected with a direct current negative electrode, and a direct current closed loop is formed by the second positive voltage electrode (2), the carrier fluid in the in-line channel (7) and the second negative voltage electrode (4).
2. The micron particle alignment apparatus of claim 1, wherein: there is an inlet pressure at the first open port (5) pushing the carrier fluid to transport particles in the carrier fluid into the in-line channel (7).
3. The micron particle alignment apparatus of claim 1, wherein: the thin film electrode is made of ITO.
4. The micron particle alignment apparatus of claim 1, wherein: the thin film electrode is made of metal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020044435.7U CN211896990U (en) | 2020-01-09 | 2020-01-09 | Micron particle arrangement device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020044435.7U CN211896990U (en) | 2020-01-09 | 2020-01-09 | Micron particle arrangement device |
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| CN211896990U true CN211896990U (en) | 2020-11-10 |
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| CN202020044435.7U Expired - Fee Related CN211896990U (en) | 2020-01-09 | 2020-01-09 | Micron particle arrangement device |
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- 2020-01-09 CN CN202020044435.7U patent/CN211896990U/en not_active Expired - Fee Related
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Granted publication date: 20201110 |