CN204724184U - A kind of dielectrophoretic particles sorting micro-fluidic chip - Google Patents
A kind of dielectrophoretic particles sorting micro-fluidic chip Download PDFInfo
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- CN204724184U CN204724184U CN201520448390.9U CN201520448390U CN204724184U CN 204724184 U CN204724184 U CN 204724184U CN 201520448390 U CN201520448390 U CN 201520448390U CN 204724184 U CN204724184 U CN 204724184U
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Abstract
The utility model discloses a kind of dielectrophoretic particles sorting micro-fluidic chip, first micro-fluidic chip soft lithographic technique is utilized, obtain the effigurate micro through hole of tool and microchannel, in conjunction with the opening and closing action of plating solution magnetic valve, the topochemistry realizing microchannel interior walls is gold-plated or silver-plated, forms cladding region.Coating electrode in cladding region forms closed non-uniform electric field region together with the plane electrode after energising, the variable electromagnetic force of size is applied by shape control magnetic valve above coating electrode, cause the deformation of coating electrode, the electric-field intensity distribution in non-uniform electric field region is caused to change, and the change causing dielectrophoretic force to distribute.The amplitude of the utility model without the need to regulating external signal source to change external electric field, and only need the size by controlling shape control magnetic valve electromagnetic force, can realize, to the non-field intensity in uniform electric field region and the adjustment of Electric Field Distribution, realizing the sorting to different attribute dielectric particle.
Description
Technical field
The utility model relates to particle sorting technology field, is specially a kind of dielectrophoretic particles sorting micro-fluidic chip.
Background technology
Microfluidic chip technology is integrated into the basic operation unit such as sample preparation, pretreatment, reaction, separation, detection that is biological, chemical analysis processes on the chip of one piece of micro-meter scale, the operation such as transport, mixing, sorting, enrichment, logical operation of fluid, micro-nano particle or drop can be realized, to the development of life science and information science, there is important strategic importance.Under non-uniform electric field, in solution, the dielectric particle (as polymer particle, various kinds of cell, bacterium, virus, DNA, drop etc.) of micro/nano-scale can be subject to the effect of dielectrophoresis (DEP) power and to the strongest or the most weak region clustering of electric-field intensity, be called positive dielectrophoresis (pDEP) and negative dielectrophoresis (nDEP) effect.This technology have applicability wide, be easy to manipulation, the advantage such as rapidly and efficiently, be usually used in fast enriching and the detection of various biological cell and DNA fragmentation on micro-fluidic chip, in fields such as the pretreatment of biological sample, the Rapid&Early diagnosis of disease, there is huge application potential.
For the solution containing multiple micro-nano particle, can, according to particle difference in size, dielectric properties (polarizability) etc., realize being separated and sorting the dielectrophoresis of intended particle.Application number is 201110162637.7 disclose a kind of micro blood cell separation device and using method thereof: by signal generator, sample-adding pump, separating chips and the synergy of cell harvestor after being separated, achieve the separation of micro blood cell.But the adjustment of the core technology dielectrophoresis of the method has been come by additional signal generator, by artificially regulating amplitude and the frequency of ac signal, reach the object that haemocyte is separated, the amplitude range of the signal of telecommunication is limited to the characteristic of signal generator itself, is difficult to flexible modulation.
Utility model content
For the shortcoming of prior art, the technical problem that the utility model quasi-solution is determined is: propose a kind of dielectrophoretic particles sorting micro-fluidic chip, first micro-fluidic chip soft lithographic technique is utilized, obtain the effigurate micro through hole of tool and microchannel, in conjunction with the opening and closing action of plating solution magnetic valve, the topochemistry realizing microchannel interior walls is gold-plated or silver-plated, forms cladding region.Coating electrode in cladding region forms closed non-uniform electric field region together with the plane electrode after energising, the variable electromagnetic force of size is applied by shape control magnetic valve above coating electrode, cause the deformation of coating electrode, the electric-field intensity distribution in non-uniform electric field region is caused to change, and the change causing dielectrophoretic force to distribute.The amplitude of the utility model without the need to regulating external signal source to change external electric field, and only need the size by controlling shape control magnetic valve electromagnetic force, can realize, to the non-field intensity in uniform electric field region and the adjustment of Electric Field Distribution, realizing the sorting to different attribute dielectric particle.
The technical scheme that the utility model solve the technical problem is: provide a kind of dielectrophoretic particles sorting micro-fluidic chip, it is characterized in that described micro-fluidic chip comprises plane electrode layer, microchannel layers and key-course; Described key-course is positioned on microchannel layers, and described microchannel layers is positioned on plane electrode layer;
Described plane electrode layer comprises electrode substrate, plane electrode and Wiring area; Described plane electrode is deposited in electrode substrate; One end, described Wiring area is connected with plane electrode, other end external power supply;
Described microchannel layers comprises microchannel and micro through hole; Described micro through hole comprises plating solution entrance, plating solution outlet, sorting solution injection port and sorting solution outlet; Described microchannel comprises plating solution microchannel and sorting solution microchannel; Described plating solution microchannel is configured with plating solution magnetic valve station, described sorting solution microchannel is configured with sorting solution electromagnetic valve station; Described plating solution microchannel forms cladding region by conventional coating technology coating; Described cladding region has coating electrode, and coating electrode forms closed non-uniform electric field region together with the plane electrode after energising;
Described key-course comprises key-course substrate, plating solution magnetic valve, sorting solution electromagnetic valve and shape control magnetic valve; The effect of described key-course substrate is for the installation of plating solution magnetic valve, sorting solution electromagnetic valve and shape control magnetic valve positions; Described plating solution magnetic valve is installed on plating solution magnetic valve station; Described sorting solution electromagnetic valve is installed on sorting solution electromagnetic valve station; Described shape control magnetic valve is placed on coating electrode, external adjustment power supply.
Compared with prior art, the beneficial effects of the utility model are: the amplitude of the utility model without the need to regulating external signal source to change external electric field, and only need the size by controlling shape control magnetic valve electromagnetic force, can realize the non-field intensity in uniform electric field region and the adjustment of Electric Field Distribution, realize the sorting to different attribute dielectric particle, and coating electrode deformation process is reversible, controlled; Microfluidic chip structure of the present utility model is reasonable, and layout is proper, and manufacturing process is simple, with low cost.
Accompanying drawing explanation
Fig. 1 is that the master of a kind of embodiment of the utility model dielectrophoretic particles sorting micro-fluidic chip looks overall structure schematic diagram; (wherein: 1, plane electrode layer; 2 microchannel layers; 3, key-course)
Fig. 2 is the main TV structure schematic diagram of plane electrode layer of a kind of embodiment of the utility model dielectrophoretic particles sorting micro-fluidic chip; (wherein: 11, electrode substrate; 12, plane electrode; 13, Wiring area)
Fig. 3 is the main TV structure schematic diagram of microchannel layers of a kind of embodiment of the utility model dielectrophoretic particles sorting micro-fluidic chip; (wherein: 211, plating solution entrance; 212, plating solution outlet; 221, the first plating solution magnetic valve station; 222, the second plating solution magnetic valve station; 231, sorting solution injection port; 232, the first sorting solution outlet; 233, the second sorting solution outlet; 234, the 3rd sorting solution outlet; 241, the first sorting solution electromagnetic valve station; 242, the second sorting solution electromagnetic valve station; 243, the 3rd sorting solution electromagnetic valve station the 244, the 4th sorting solution electromagnetic valve station; )
Fig. 4 is the main TV structure schematic diagram of key-course of a kind of embodiment of the utility model dielectrophoretic particles sorting micro-fluidic chip; (wherein: 31, key-course substrate; 34, shape control magnetic valve; 321, the first plating solution magnetic valve; 322, the second plating solution magnetic valve; 331, the first sorting solution electromagnetic valve; 332, the second sorting solution electromagnetic valve; 333, the 3rd sorting solution electromagnetic valve; 334, the 4th sorting solution electromagnetic valve)
Fig. 5 is the cladding region schematic diagram of a kind of embodiment of the utility model dielectrophoretic particles sorting micro-fluidic chip; (wherein: 25, cladding region; 251, coating electrode)
Fig. 6 be the utility model dielectrophoretic particles sorting micro-fluidic chip Fig. 5 shown in dashed region look up structural representation;
Fig. 7 is strong electric field region and the weak electric field region formation basic theory figure of a kind of embodiment of the utility model dielectrophoretic particles sorting micro-fluidic chip; (wherein: a-quadrant-strong electric field region; B region and C region-weak electric field region; F is the electromagnetic force of shape control magnetic valve 34)
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing thereof, the utility model method is further described:
The utility model provides a kind of dielectrophoretic particles sorting micro-fluidic chip (see Fig. 1-6), comprises plane electrode layer 1, microchannel layers 2 and key-course 3; Described key-course 3 is positioned on microchannel layers 2, and described microchannel layers 2 is positioned on plane electrode layer 1;
Described plane electrode layer 1 comprises electrode substrate 11, plane electrode 12 and Wiring area 13; Described plane electrode 12 is deposited in electrode substrate 11; Described electrode substrate 11 material is glass, acrylic, epoxy resin, ABS etc., and size dimension is 5mm ~ 100mm, thickness 1 ~ 3mm; The material of described plane electrode 12 is conductive material, and can be copper, aluminium, gold, silver, ITO, IFO etc., thickness be 100um ~ 1mm, and width is 10 ~ 400um; One end, described Wiring area 13 is connected with plane electrode 12, other end external power supply, for providing AC power for plane electrode 12;
Described microchannel layers 2 comprises microchannel and micro through hole; The material of described microchannel layers 2 is dimethyl silicone polymer (PDMS), and size dimension is 5mm ~ 100mm, and thickness 1 ~ 3mm makes micro through hole and microchannel by soft lithographic technique; Described micro through hole comprises plating solution entrance 211, plating solution outlet 212, sorting solution injection port 231 and sorting solution outlet, is respectively used to the sample introduction of plating solution and sorting solution and goes out sample; Described sorting solution outlet has three, is specially the first sorting solution outlet 232, second sorting solution outlet 233 and the 3rd sorting solution outlet 234; The degree of depth of described microchannel is 10 ~ 500um, and width is 20 ~ 800um, for the flowing of plating solution and sorting solution provides passage, comprises plating solution microchannel and sorting solution microchannel; Described plating solution microchannel is configured with plating solution magnetic valve station, described sorting solution microchannel is configured with sorting solution electromagnetic valve station; The diameter of described plating solution magnetic valve station and solution electromagnetic valve station is 1 ~ 10mm, installs corresponding magnetic valve for coordinating; Described plating solution magnetic valve station has two, specifically the first plating solution magnetic valve station 221 and the second plating solution magnetic valve station 222; Described sorting solution electromagnetic valve station has four, is specially the first sorting solution electromagnetic valve station 241, second sorting solution electromagnetic valve station the 242, the 3rd sorting solution electromagnetic valve station 243 and the 4th sorting solution electromagnetic valve station 244; Described plating solution microchannel forms cladding region 25 (shadow region shown in Figure 5) after conventional coating technology coating; Described cladding region 25 has coating electrode 251 (dashed region shown in Figure 5); Described coating electrode 251 forms closed non-uniform electric field region together with the plane electrode 12 after energising, makes the various dielectric particles flowed through in the solution of coating electrode 251 be subject to the effect of dielectrophoretic force; Vertical range between described coating electrode 251 and plane electrode 12 is 10 ~ 500um.
The material of described key-course 3 is glass, acrylic, epoxy resin, ABS etc., and size dimension is 5mm ~ 100mm, thickness 1 ~ 3mm, concrete size reference plane electrode layer 1 and microchannel layers 2, accomplishes that size is mated; Described key-course 3 comprises key-course substrate 31, plating solution magnetic valve, sorting solution electromagnetic valve and shape control magnetic valve 34; The effect of described key-course substrate 31 is for the installation of plating solution magnetic valve, sorting solution electromagnetic valve and shape control magnetic valve 34 is accurately located; Described plating solution magnetic valve has two, be specially the first plating solution magnetic valve 321 and the second plating solution magnetic valve 322, first plating solution magnetic valve 321 is installed on the first plating solution magnetic valve station 221, and the second plating solution magnetic valve 322 is installed on the second plating solution magnetic valve station 222; Described sorting solution electromagnetic valve has four, be specially the first sorting solution electromagnetic valve 331, second sorting solution electromagnetic valve 332, the 3rd sorting solution electromagnetic valve 333 and the 4th sorting solution electromagnetic valve 334, correspondence is installed on the first sorting solution electromagnetic valve station 241, second sorting solution electromagnetic valve station the 242, the 3rd sorting solution electromagnetic valve station 243 and the 4th sorting solution electromagnetic valve station 244 respectively, for controlling the break-make of sorting solution, carry out the sorting of solution particle; Described shape control magnetic valve 34 is placed on coating electrode 251, external adjustment power supply, electromagnetic force F can be formed, the size of electromagnetic force F is regulated by the voltage of external adjustment power supply, by the change of electromagnetic force, pressure and deformation are produced to coating electrode 251, control with this electric-field intensity distribution that coating electrode 251 forms closed non-uniform electric field region together with the plane electrode 12 after energising.
The generation type of described cladding region 25 is: the first plating solution magnetic valve 321 and the second plating solution magnetic valve 322 are opened, all sorting solution electromagnetic valve are closed, then pass into plating solution at plating solution entrance 211, under atmospheric pressure, form cladding region 25 (see Fig. 5 shadow region).
The operation principle of the utility model dielectrophoretic particles sorting micro-fluidic chip and process are (see Fig. 5,7):
Described coating electrode 251 (shown in Fig. 5 dashed region) forms closed non-uniform electric field region together with the plane electrode 12 after energising, under the control of described shape control magnetic valve 34 electromagnetic force F, coating electrode 251 can produce deformation in various degree, the vertical range of deformation quantity scope between plane electrode 12 and coating electrode 251 of coating electrode 251 1% ~ 99% between, wherein the size of electromagnetic force F is regulated by the voltage of external adjustment power supply.Deformation causes non-uniform electric field intra-zone electric-field intensity to change, form strong electric field region (a-quadrant, corresponding second sorting solution outlet 233) and weak electric field region (B region and C region, corresponding first sorting solution outlet 232 and the 3rd sorting solution outlet 234 respectively), make to flow through the dielectrophoretic force that the various dielectric particles in the solution in coating electrode 251 region are subject to change, it is made to deflect respectively, flow out from different sorting solution outlets, realize sorting function.
When sorting starts, first plating solution magnetic valve 321 and the second plating solution magnetic valve 322 are closed, all sorting solution electromagnetic valve are opened, the solution containing multiple different dielectric attribute particle is passed at sorting solution injection port 231, particle sorting can be completed under the effect of non-uniform electric field, particle after sorting flows out from different sorting solution outlet 232 ~ 234 respectively, realizes sorting.
Embodiment 1
First plating solution magnetic valve 321 and the second plating solution magnetic valve 322 are closed, all sorting solution electromagnetic valve are opened, pass into the solution containing anyway yeast cell at sorting solution injection port 231, yeast cell is subject to the dielectrophoretic force of different attribute (just or negative) respectively anyway.Microchannel width is 200um, degree of depth 50um herein, and the diameter of all plating solution magnetic valve stations and sorting solution electromagnetic valve station is 3mm, and the vertical range between coating electrode and plane electrode is 15um.Dead cell flows out from the first sorting solution outlet 232 and the 3rd sorting solution outlet 234, corresponding weak electric field region; Living cells flows out from the second sorting solution outlet 233, corresponding strong electric field region.
Embodiment 2
First plating solution magnetic valve 321 and the second plating solution magnetic valve 322 are closed, all sorting solution electromagnetic valve are opened, pass into the human blood containing 1:50 dilution at sorting solution injection port 231, red blood cell and leucocyte are subject to the dielectrophoretic force of different attribute (just or negative) respectively.Microchannel width is 120um, degree of depth 60um herein, and the diameter of all plating solution magnetic valve stations and sorting solution electromagnetic valve station is 8mm, and the vertical range between coating electrode and plane electrode is 20um.Red blood cell flows out from the first sorting solution outlet 232 and the 3rd sorting solution outlet 234, corresponding weak electric field region; Leucocyte flows out from the second sorting solution outlet 233, corresponding strong electric field region.
The utility model is not addressed part and is applicable to prior art.
Claims (4)
1. a dielectrophoretic particles sorting micro-fluidic chip, is characterized in that described micro-fluidic chip comprises plane electrode layer, microchannel layers and key-course; Described key-course is positioned on microchannel layers, and described microchannel layers is positioned on plane electrode layer;
Described plane electrode layer comprises electrode substrate, plane electrode and Wiring area; Described plane electrode is deposited in electrode substrate; One end, described Wiring area is connected with plane electrode, other end external power supply;
Described microchannel layers comprises microchannel and micro through hole; Described micro through hole comprises plating solution entrance, plating solution outlet, sorting solution injection port and sorting solution outlet; Described microchannel comprises plating solution microchannel and sorting solution microchannel; Described plating solution microchannel is configured with plating solution magnetic valve station, described sorting solution microchannel is configured with sorting solution electromagnetic valve station; Described plating solution microchannel forms cladding region by conventional coating technology coating; Described cladding region has coating electrode, and coating electrode forms closed non-uniform electric field region together with the plane electrode after energising;
Described key-course comprises key-course substrate, plating solution magnetic valve, sorting solution electromagnetic valve and shape control magnetic valve; The effect of described key-course substrate is for the installation of plating solution magnetic valve, sorting solution electromagnetic valve and shape control magnetic valve positions; Described plating solution magnetic valve is installed on plating solution magnetic valve station; Described sorting solution electromagnetic valve is installed on sorting solution electromagnetic valve station; Described shape control magnetic valve is placed on coating electrode, external adjustment power supply.
2. dielectrophoretic particles sorting micro-fluidic chip according to claim 1, it is characterized in that the degree of depth of described microchannel is 10 ~ 500um, width is 20 ~ 800um.
3. dielectrophoretic particles sorting micro-fluidic chip according to claim 1, is characterized in that the diameter of described plating solution magnetic valve station and sorting solution electromagnetic valve station is 1 ~ 10mm.
4. dielectrophoretic particles sorting micro-fluidic chip according to claim 1, is characterized in that the vertical range between described coating electrode and plane electrode is 10 ~ 500um.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520448390.9U CN204724184U (en) | 2015-06-26 | 2015-06-26 | A kind of dielectrophoretic particles sorting micro-fluidic chip |
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| CN201520448390.9U CN204724184U (en) | 2015-06-26 | 2015-06-26 | A kind of dielectrophoretic particles sorting micro-fluidic chip |
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| CN204724184U true CN204724184U (en) | 2015-10-28 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104923322A (en) * | 2015-06-26 | 2015-09-23 | 河北工业大学 | Dielectrophoresis particle separation micro-fluidic chip |
| CN106378213A (en) * | 2016-05-05 | 2017-02-08 | 海南大学 | Dielectrophoresis-based chip for separation of deformable microparticles |
-
2015
- 2015-06-26 CN CN201520448390.9U patent/CN204724184U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104923322A (en) * | 2015-06-26 | 2015-09-23 | 河北工业大学 | Dielectrophoresis particle separation micro-fluidic chip |
| CN106378213A (en) * | 2016-05-05 | 2017-02-08 | 海南大学 | Dielectrophoresis-based chip for separation of deformable microparticles |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20151028 Effective date of abandoning: 20160608 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |