KR20170016900A - Apparatus for separating particle comprising divisible multiple panels - Google Patents

Abstract

The present invention relates to an apparatus assembly for separating fine particles. A novel structured apparatus assembly for separating fine particles comprises: a first panel having a magnetic microstructure applying magnetophoretic power to fine particles; and a second panel through which a sample having fine particles separated from the first panel passes. The first panel having the magnetic microstructure is reusable.

Description

TECHNICAL FIELD [0001] The present invention relates to a fine particle separating device assembly including a plurality of detachable panels.

The present invention relates to a fine particle separating apparatus assembly including a plurality of detachable panels, and more particularly, to a fine particle separating apparatus comprising a first panel including a magnetic microstructure for applying a magnetogrinding force to a fine particle to be separated, Wherein the first panel including the magnetic microstructure is manufactured in a mutually separated form so that the second panel through which the sample containing the magnetic microstructure is passed can be recycled several times, .

Recently, with the development of microfabrication technology, microelectronic devices for concentrating, assembling and separating fine particles have been actively developed for applications in biological, chemical and new materials fields.

In such microelectronic devices, various electrokinetic principles such as electrophoresis, dielectrophoresis, and electro-osmosis are used to precisely drive or separate fine particles, proteins, cells, bacteria, .

The magnetophoresis separation technique using high gradient magnetic separation (HGMS) has advantages of simple structure, high efficiency, easy to use, and no hydrolysis characteristic compared to dielectrophoresis. It is a field that is constantly being studied.

The conventional magnetophoresis method has a magnetic micro-structure region for amplifying a magnetic field gradient applied by the external magnetic energy source and a magnetic energy source for applying a magnetic field for separating the magnetic sample to be separated, A magnetic field is applied from a magnetic energy source to a sample having magnetic properties, and the sample is separated according to the magnetic density gradient.

Korean Patent No. 10-0791036 discloses a method of disposing a desired particle by disposing a ferromagnetic structure on the side of a microfluidic channel and applying an external magnetic field in a direction perpendicular to the flow of the sample. In the conventional apparatus for separating and capturing fine particles, a bottom panel including a magnetic microstructure and a channel through which a sample including a fluid and fine particles move are integrally formed. Accordingly, the bottom panel including the magnetic microstructure that applies the magnetic force to the fine particles is not contaminated by the sample but can not be used again after one use. As a result, the entire lower panel and the upper panel must be manufactured Therefore, there is a problem that the cost of manufacturing the fine particle device increases.

Disclosure of the Invention In order to solve the problems of the prior art as described above, the present invention has been made in view of the above problems, and it is an object of the present invention to provide a micro- It is another object of the present invention to provide a fine particle separator having a novel structure capable of repeatedly using a first panel including the magnetic microstructure to control the path of fine particles to be separated.

The present invention has been made to solve the above problems

A first panel comprising a magnetic microstructure; And a second panel detachable from the first panel and including a microstructure through which the sample passes; And a microparticle separation device assembly.

FIG. 1 and FIG. 2 show a sectional view and a separation view of the apparatus for separating fine particles according to the present invention.

1, the apparatus for separating fine particles according to the present invention includes a first panel 200 including a magnetic microstructure, a second panel 100 separated from the first panel and including a microstructure through which the sample passes, And a thin film portion 410 formed at a portion where the first panel 100 and the second panel 200 are in contact with each other.

In the fine particle separating device assembly according to the present invention, the magnetic microstructures included in the first panel are not particularly limited as long as they are materials capable of forming a magnetic field.

In the apparatus for separating fine particles according to the present invention, the magnetic microstructures included in the first panel may be arranged in such a manner that the direction of the magnetic field generated in order to separate the fine particles And forming a pattern inclined at a predetermined angle.

In the apparatus for separating fine particles according to the present invention, the magnetic microstructure included in the first panel may include a pattern formed in a direction perpendicular to a direction in which a sample flows into a microchannel formed in the second panel .

As shown in FIGS. 1 and 2, the second panel 100 according to the present invention includes an inlet 800 for injecting a sample containing fine particles into the microchannel, and an injection port 800 for discharging the separated fine particles from the microchannel And an outlet 900 for the outlet.

1 and 2, in the second panel of the apparatus for separating fine particles according to the present invention, a portion where the first panel contacts with the second panel is formed of a thin film portion 410 having a thickness of 50 μm or less .

That is, although the first panel 200 and the second panel 100 are separated from each other in the device for separating fine particles according to the present invention, the magnetophoretic force induced by the magnetic microstructure of the first panel And a thin film portion 410 having a thickness of 50 μm or less is formed between the second panel and the first panel so as to be transmitted to the sample passing through the second panel.

In the apparatus for separating fine particles according to the present invention, the thin film part 410 may be manufactured separately from the second panel as shown in FIG. 1, or may be manufactured integrally with the second panel as shown in FIG. 2 It is possible.

In addition, in the apparatus for separating fine particles according to the present invention, the material for forming the thin film portion is not particularly limited, and plastic materials such as PET, PI, PE, PP, PMMA, glass or the like may be used.

FIG. 3 schematically shows a second panel including a microchannel through which a sample of the apparatus for separating fine particles according to the present invention passes. In FIG. 3, the sample separating portion and the sample capturing portion of FIG. The structures of various embodiments are shown in Figs. 4 and 5, respectively.

The apparatus for separating fine particles according to the present invention comprises a coupling part for coupling the separated first panel and the second panel between the first panel and the second panel.

In the apparatus for separating fine particles according to the present invention, the coupling portion is not particularly limited, and includes a vacuum applying portion and a magnet. In the apparatus for separating fine particles according to the present invention, the fine particle separator may include concave and convex portions formed to be coupled to the first panel and the second panel.

FIG. 6 shows various coupling parts in the apparatus for separating fine particles according to the present invention. 6 (a), 6 (b) and 6 (c), the coupling part may be the vacuum applying part 500 or may be the magnet 600 as shown in FIG. 6 (d). 6 (a), 6 (b) and 6 (c), the vacuum applying unit 500 may be formed on only one of the first panel and the second panel, or may be formed on both sides of the first panel and the second panel .

 6 (e) and 6 (f), the coupling portion may include a receiving portion for receiving the second panel, the first panel being recessed at the central portion thereof, or the first and second panels may correspond to each other And can be selected by those skilled in the art to fix the first panel and the second panel.

The apparatus for separating fine particles according to the present invention may further comprise a plurality of separated first and second panels connected to each other. That is, as shown in FIG. 11, in the apparatus for separating fine particles according to the present invention, the first panel includes a separated first 1-1 panel, a first 1-2 panel, , And 1-n (n is an integer of 1 or more) panels are connected in a horizontal direction. In the apparatus for separating fine particles according to the present invention, the separated second-1-th panel, the second-2-th panel, , And the second-n panels are continuously connected in the horizontal direction. The connection means between the first panel connected horizontally is not particularly limited, and it is possible to connect the injection port for injecting the sample including the fine particles into the microchannel through the connection portion 810.

In the apparatus for separating fine particles according to the present invention, the first-n panel is vertically connected to the second-n panel. In the apparatus for separating fine particles according to the present invention, the first-n panel is in contact with the second-n panel and the thin film part. In the apparatus for separating fine particles according to the present invention, the first panel may be located above the second panel, or the second panel may be located above the first panel.

In the apparatus for separating fine particles according to the present invention, the patterns included in the magnetic microstructures may be formed to be different from each other in a plurality of connected panels, and a portion for cell separation and a portion for cell capture may be formed in different shapes .

As shown in FIG. 4, in the apparatus for separating fine particles according to the present invention, the magnetic microstructures included in the first panel include patterns formed in a direction perpendicular to the direction in which the sample flows.

In the apparatus for separating fine particles according to the present invention, the magnetic microstructures included in the first-n panel are perpendicular to the sample flow direction.

It is preferable that the panel including the pattern formed in the direction perpendicular to the direction in which the sample is introduced is formed last since the cells can no longer flow by the magnetic pattern formed in the direction perpendicular to the direction in which the sample flows .

The apparatus for separating fine particles according to the present invention further comprises a second panel including a magnetic microstructure capable of being reused, the second panel being made of a single piece, And may further include an adjustment unit for aligning the alignment. The form of the adjustment part is not particularly limited.

The apparatus for separating fine particles according to the present invention can be used only once according to a sample, since the first panel including the magnetic microstructure is separated from the second panel including the microstructure through which the sample passes, , The first panel including the magnetic microstructure can be used repeatedly many times, which is economical.

1 and 2 show a microparticle separation device assembly according to the present invention.
3 to 5 show patterns of the magnetic microstructures of the first panel and the second panel of the apparatus for separating fine particles according to an embodiment of the present invention.
FIG. 6 shows various embodiments of the coupling portion of the apparatus for separating fine particles according to the present invention.
FIGS. 7 to 9 show a fine particle separating apparatus manufactured according to an embodiment of the present invention.
FIG. 10 shows a state where cells are separated using a microparticle separation apparatus manufactured according to an embodiment of the present invention.
11 and 12 show a device for separating fine particles produced according to another embodiment of the present invention.
FIG. 13 shows a state in which cells are separated using the apparatus for separating fine particles according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail by way of examples. However, the present invention is not limited by the following examples.

< Manufacturing example  1>

FIG. 7 shows a first panel and a second panel separately manufactured. 8 shows a state in which the first panel and the second panel separated and manufactured are connected to a vacuum pump and vacuum is applied thereto. FIG. 8 shows a state in which a magnet is disposed on the second panel, Fig.

As shown in FIG. 7, the first panel 200 includes a magnetic material microstructure 300 and a vacuum applying unit 510 to which a vacuum can be applied to the periphery of the magnetic material microstructure. The second panel 100 includes a microchannel structure 110 that is separate from the first panel and can be separately captured as the sample passes.

9 shows a state in which the first panel and the second panel separated and manufactured as described above are placed on the ferromagnetic body and the magnet 600 is disposed on the second panel so that the first panel and the second panel are bonded by the thin film portion Respectively.

< Experimental Example  1>

FIG. 10 shows the fluorescence of the cells captured and captured in the capture unit by using the separated fine particle separating apparatus assembly according to the present invention prepared in Production Example 1, and FIG.

< Manufacturing example  2>

As shown in the schematic view in FIG. 11, a fine particle separating apparatus assembly in which a plurality of separated panels were connected was manufactured.

A first 1-1 panel 210 including a magnetic microstructure 310 for sample separation, a magnetic microstructure 320 separated from the first 1-1 panel and formed in a direction perpendicular to the sample inlet direction for sample capture, ) Was included in the second panel.

In addition, a second-1 panel 110 connected to the first 1-1 panel 210 and including a channel for separating the sample, a second-1 panel 110 connected to the first 1-2 panel, And a 2-2 panel 120 including a chamber were respectively prepared.

After the thin film part is manufactured, the first assembly 10 to which the 1-1 panel and the 2-1 panel are in contact with each other with the thin film part therebetween, the second assembly 10-1 to which the 1-2 panel and the 2-2 panel are connected, The sample separated on the 2-1 panel can be captured on the 2-2 panel by connecting the first assembly 10 and the second assembly 10-1 with the connection part 810 And the connected state is shown in FIG.

Cells were separated and captured using the microparticle separation device assembly prepared in the above production example, and fluorescence photographs of the cells captured in the capture part are shown in FIG.

Claims (15)

A first panel comprising a magnetic microstructure; And
A microchannel structure separated from the first panel and through which the sample passes; A second panel including a bonded thin film portion;
Lt; RTI ID = 0.0 &gt;
The method according to claim 1,
Wherein the magnetic microstructures included in the first panel are inclined at a predetermined angle with respect to the flow direction of the cells in the second panel.
The method according to claim 1,
Wherein the magnetic microstructure contained in the first panel includes a pattern formed in a direction perpendicular to a direction in which the sample is introduced.
The method according to claim 1,
The thin film portion is a fine particle separating device assembly
5. The method of claim 4,
Wherein the thin film part is made of PET, PI, PE, PP, PMMA or glass.
The method according to claim 1,
Further comprising an engaging portion for engaging the first panel and the second panel.
The method according to claim 6,
Wherein the engaging portion comprises a vacuum applying portion, or a magnet.
The method according to claim 1,
Wherein the engaging portion includes a concave-convex portion formed in correspondence with the first panel and the second panel,
The method according to claim 1,
The first panel includes n first 1-1 panels, a 1-2 panel, , And a first-n panel is connected
The method according to claim 1,
The second panel is divided into n second-1 panels, second-2 panels, ... , And a second-n panel is connected
11. The method according to claim 9 or 10,
Wherein the first-n panel is in contact with the second-n panel and the thin film portion,
12. The method of claim 11,
Wherein the magnetic microstructures included in the first-n panel are perpendicular to the sample flow direction.
The method according to claim 1,
Wherein the first panel is located on top of the second panel.
The method according to claim 1,
Wherein the first panel is located below the second panel.
The method according to claim 1,
Wherein the first panel and the second panel further comprise an adjuster for aligning the magnetic permeability channel with the magnetic microstructure.

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