CN112111480A - Method for prolonging shelf life of paecilomyces lilacinus by using double-layer embedding technology - Google Patents
Method for prolonging shelf life of paecilomyces lilacinus by using double-layer embedding technology Download PDFInfo
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- 241001465752 Purpureocillium lilacinum Species 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 31
- 239000002245 particle Substances 0.000 claims abstract description 17
- 239000003223 protective agent Substances 0.000 claims abstract description 17
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 15
- 229910021536 Zeolite Inorganic materials 0.000 claims abstract description 11
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000010457 zeolite Substances 0.000 claims abstract description 11
- 239000002861 polymer material Substances 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims description 19
- 238000002156 mixing Methods 0.000 claims description 15
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 11
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- 159000000007 calcium salts Chemical class 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical group [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 6
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims description 6
- 239000002151 riboflavin Substances 0.000 claims description 6
- 229960002477 riboflavin Drugs 0.000 claims description 6
- 235000019192 riboflavin Nutrition 0.000 claims description 6
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 5
- 239000004368 Modified starch Substances 0.000 claims description 5
- 229920000881 Modified starch Polymers 0.000 claims description 5
- 229960005070 ascorbic acid Drugs 0.000 claims description 5
- 235000010323 ascorbic acid Nutrition 0.000 claims description 5
- 239000011668 ascorbic acid Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 235000019426 modified starch Nutrition 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 239000000661 sodium alginate Substances 0.000 claims description 5
- 235000010413 sodium alginate Nutrition 0.000 claims description 5
- 229940005550 sodium alginate Drugs 0.000 claims description 5
- 229920003169 water-soluble polymer Polymers 0.000 claims description 5
- PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 claims description 4
- 235000012209 glucono delta-lactone Nutrition 0.000 claims description 4
- 229960003681 gluconolactone Drugs 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 3
- 229960002143 fluorescein Drugs 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- 229920001661 Chitosan Polymers 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 229920002401 polyacrylamide Polymers 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000004321 preservation Methods 0.000 abstract description 4
- 238000003860 storage Methods 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000010410 layer Substances 0.000 description 22
- 230000000694 effects Effects 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 241000243785 Meloidogyne javanica Species 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 230000009467 reduction Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 239000000648 calcium alginate Substances 0.000 description 2
- 235000010410 calcium alginate Nutrition 0.000 description 2
- 229960002681 calcium alginate Drugs 0.000 description 2
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000004763 spore germination Effects 0.000 description 2
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000002888 effect on disease Effects 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 125000001452 riboflavin group Chemical group 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
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- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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Abstract
In order to solve the problems of short shelf life, low spore survival rate, high storage cost, inconvenience in transportation, large-area popularization and use and the like in the preservation of the paecilomyces lilacinus, the invention discloses a method for prolonging the shelf life of the paecilomyces lilacinus by using a double-layer embedding technology, particles with a double-layer structure are mainly formed by the paecilomyces lilacinus, a water-soluble high polymer material, an ultraviolet protective agent, a cross-linking agent, zeolite and water, and the paecilomyces lilacinus spores are wrapped by the inner-layer structure, so that nutrient substances can be provided for the paecilomyces lilacinus, and the survival rate of the paecil; the outer layer structure increases the mechanical strength, can more effectively protect microorganisms, prolongs the quality guarantee period of the paecilomyces lilacinus, and is convenient to transport and popularize and use in a large area.
Description
Technical Field
The invention belongs to the technical field of biocontrol bacteria preservation, and particularly relates to a method for prolonging the quality guarantee period of paecilomyces lilacinus by using a double-layer embedding technology.
Background
The control of plant diseases has always been the major focus in agricultural production work. In agriculture, crop root knot nematode disease is caused along with the growth of crop planting time in the same place, in recent years, a large amount of crop yield reduction is caused by a plurality of plant diseases such as blight, root rot and the like caused by the plant root knot nematode, the yield reduction is generally 10% -30%, the serious yield reduction is more than 50% -70%, even no harvest is realized, and the annual loss caused by the root knot nematode disease in China is more than 700 million yuan according to incomplete statistics.
The paecilomyces lilacinus is inhabitation bacteria of various plant roots in soil and is an important natural enemy of plant parasitic nematodes, and the paecilomyces lilacinus is known to have a good effect on preventing and treating plant root-knot nematodes and simultaneously has a prevention and treatment effect on diseases such as fusarium oxysporum caused by various kinds of soil. The paecilomyces lilacinus has the characteristics of high efficiency, broad spectrum, long effect, safety, no pollution, no residue and the like, and cannot cause damage to the environment; in addition, paecilomyces lilacinus can also secrete plant hormones in the growth process, and has remarkable promotion and regulation effects on the growth of plants, so that the paecilomyces lilacinus is widely applied to development of efficient, stable and safe biopesticides.
The prevention and control effect of the paecilomyces lilacinus on plant pathogenic bacteria is mainly realized through spore action. Paecilomyces lilacinus exists in the form of spore, spore germinates to form hypha to invade host under proper temperature and proper nutrition condition to realize disease resistance, the hypha of Paecilomyces lilacinus surrounds the whole egg, the end of the hypha becomes thick, the surface layer of the egg shell is cracked due to the activity of exogenous metabolites and fungal chitinase, and then the fungus invades and replaces the shell. Therefore, the spore survival rate of the paecilomyces lilacinus determines the using effect of the product. However, the paecilomyces lilacinus spores are poor in stress resistance and easy to die, and the using effect of the paecilomyces lilacinus microbial inoculum is seriously influenced.
At present, the common preservation methods of paecilomyces lilacinus spores are as follows: 1. freeze-drying preservation is carried out, and the cost is high; 2. calcium alginate is embedded, so that the cost is high and the calcium alginate is not easy to use; 3. the spores are directly dried or absorbed into carriers such as river sand and the like for drying and low-temperature storage, so that the transportation and the use are not easy; 4. The paecilomyces lilacinus spores are directly exposed in soil environment with uncertain factors after being mixed with activated carbon and stored at normal temperature, so that the survival rate of the spores is low easily, and a method for prolonging the quality guarantee period of the paecilomyces lilacinus is urgently needed.
Disclosure of Invention
The invention aims to solve the problems of short shelf life, low spore survival rate, high storage cost, inconvenience in transportation, large-area popularization and use and the like in the storage of paecilomyces lilacinus, and provides a method for prolonging the shelf life of the paecilomyces lilacinus by using a double-layer embedding technology.
The invention relates to a method for prolonging the quality guarantee period of paecilomyces lilacinus by using a double-layer embedding technology, which comprises the following raw materials: paecilomyces lilacinus; water-soluble high molecular material, ultraviolet protective agent, cross-linking agent, zeolite and water; the ultraviolet ray protective agent is one or more of riboflavin, fluorescein and ascorbic acid.
Preferably, the method for prolonging the shelf life of the paecilomyces lilacinus by using the double-layer embedding technology specifically comprises the following steps:
the method comprises the following steps: mixing a water-soluble polymer material and water according to a mass ratio of 1: 1-50, heating and stirring for 15-30 minutes, and cooling to room temperature to obtain a colloidal solution;
step two: the mass ratio of the colloidal solution to the cross-linking agent is 5-15: 1-5, stirring uniformly to obtain an embedding solution;
step three: uniformly stirring and mixing the paecilomyces lilacinus fermentation liquor and the ultraviolet protective agent according to the mass ratio of 1-5: 0.1-1, uniformly dripping the mixture into embedding liquid at the speed of 30-60 drops/minute, stirring while dripping, and reacting for 5-12 hours to obtain embedded particles;
step four: and uniformly mixing zeolite and the embedded particles, standing for 10-20 hours to obtain outer-layer embedded particles, and exposing in a ventilated and cool environment for 4-10 days to dry in the shade.
Preferably, the method for prolonging the shelf life of the paecilomyces lilacinus by using the double-layer embedding technology is characterized in that the bacterial concentration of the paecilomyces lilacinus is 2.0-50.0 hundred million/ml.
Preferably, the method for prolonging the shelf life of the paecilomyces lilacinus by using the double-layer embedding technology is characterized in that the water-soluble high polymer material is any one or more of modified starch, chitosan and sodium alginate agar, and the cross-linking agent is any one of polyacrylamide and calcium salt.
Preferably, the method for prolonging the shelf life of the paecilomyces lilacinus by using the double-layer embedding technology is characterized in that the calcium salt is any one of calcium carbonate, calcium chloride and calcium sulfate, and gluconolactone is added to form a slow-release calcium source.
The invention at least comprises the following beneficial effects: the invention provides a method for prolonging the quality guarantee period of paecilomyces lilacinus by using a double-layer embedding technology, wherein an inner layer structure contains one or more of ultraviolet protective agents riboflavin, fluorescein and ascorbic acid, so that the protective effect on paecilomyces lilacinus spores is enhanced, and the quality guarantee period is prolonged; in addition, the paecilomyces lilacinus spores are wrapped by the inner-layer structure, so that nutrient substances can be provided for the paecilomyces lilacinus spores, and the influence on the survival rate of the paecilomyces lilacinus spores caused by direct exposure of the paecilomyces lilacinus spores in soil is avoided; the outer structure increases mechanical strength, can more effectively protect microorganisms, and is convenient to transport and popularize and use in a large area.
Detailed Description
Example 1
A method for prolonging the shelf life of Paecilomyces lilacinus by using a double-layer embedding technology comprises the following raw materials: paecilomyces lilacinus; water-soluble high molecular material, ultraviolet protective agent, cross-linking agent, zeolite and water; the ultraviolet protective agent is riboflavin, the water-soluble high polymer material is modified starch, and the cross-linking agent is calcium salt and mainly is a mixture of calcium carbonate and gluconolactone.
Preferably, the method for prolonging the shelf life of the paecilomyces lilacinus by using the double-layer embedding technology comprises the following steps:
the method comprises the following steps: mixing modified starch and water according to the mass ratio of 1:10, heating and stirring for 15 minutes, and cooling to room temperature to obtain a colloidal solution;
step two: the mass ratio of the colloidal solution to the calcium salt is 5: 1, uniformly stirring to obtain an embedding solution;
step three: uniformly stirring and mixing 2.0 hundred million/ml of paecilomyces lilacinus fermentation liquor and an ultraviolet ray protective agent riboflavin according to a mass ratio of 1:0.3, uniformly dripping 60 drops/min into embedding liquid while stirring, and reacting for 5 hours to obtain embedded particles;
step four: and uniformly mixing zeolite and the embedded particles, standing for 10 hours to obtain outer-layer embedded particles, and exposing in a ventilated and cool environment for 4 days to dry in the shade.
Example 2
A method for prolonging the shelf life of Paecilomyces lilacinus by using a double-layer embedding technology comprises the following raw materials: paecilomyces lilacinus; water-soluble high molecular material, ultraviolet protective agent, cross-linking agent, zeolite and water; the ultraviolet ray protective agent is ascorbic acid, the water-soluble polymer material is sodium alginate, and the cross-linking agent is calcium salt, mainly a mixture of calcium chloride and gluconolactone.
Preferably, the method for prolonging the shelf life of the paecilomyces lilacinus by using the double-layer embedding technology comprises the following steps:
the method comprises the following steps: mixing sodium alginate and water according to a mass ratio of 1:5, heating and stirring for 20 minutes, and cooling to room temperature to obtain a colloidal solution;
step two: the mass ratio of the colloidal solution to the cross-linking agent is 10: 3, uniformly stirring to obtain an embedding solution;
step three: mixing the paecilomyces lilacinus fermentation liquor with the thallus concentration of 10.0 hundred million/ml and the ultraviolet protective agent uniformly according to the mass ratio of 5: 1, uniformly dripping the mixture into embedding liquid at a speed of 45 drops/minute, stirring while dripping, and reacting for 12 hours to obtain embedded particles;
step four: and uniformly mixing zeolite and the embedded particles, standing for 20 hours to obtain outer-layer embedded particles, and exposing in a ventilated and cool environment for 5 days and drying in the shade.
Example 3
A method for prolonging the shelf life of Paecilomyces lilacinus by using a double-layer embedding technology comprises the following raw materials: paecilomyces lilacinus; water-soluble high molecular material, ultraviolet protective agent, cross-linking agent, zeolite and water; the ultraviolet ray protective agent is a mixture of ascorbic acid and riboflavin, the water-soluble polymer material is a mixture of sodium alginate and modified starch, and the cross-linking agent is calcium salt and mainly calcium sulfate.
Preferably, the method for prolonging the shelf life of the paecilomyces lilacinus by using the double-layer embedding technology comprises the following steps:
the method comprises the following steps: mixing a water-soluble polymer material and water according to a mass ratio of 1: 50, heating and stirring for 30 minutes, and cooling to room temperature to obtain a colloidal solution;
step two: the mass ratio of the colloidal solution to the cross-linking agent is 15: 3, uniformly stirring to obtain an embedding solution;
step three: uniformly stirring and mixing the paecilomyces lilacinus fermentation liquor with the thallus concentration of 50.0 hundred million/ml and the ultraviolet protective agent according to the mass ratio of 3:0.3, uniformly dripping into embedding liquid at the speed of 30 drops/minute, stirring while dripping, and reacting for 10 hours to obtain embedded particles;
step four: and uniformly mixing zeolite and the embedded particles, standing for 15 hours to obtain outer-layer embedded particles, and exposing in a ventilated and cool environment for 10 days to dry in the shade.
Example 4
And (3) storing the paecilomyces lilacinus embedded particles prepared in the step 1-3 at the temperature of 0-30 ℃, and determining the germination rate. The results are given in the table below.
Table 1: step 1 the prepared granules are preserved for 12 months under the condition of 0-30 ℃ to have spore germination rate.
The result shows that the method for prolonging the quality guarantee period of the paecilomyces lilacinus by using the double-layer embedding technology is stored at different temperatures, and the spore germination rate is more than 85%.
While embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable to a variety of fields of endeavor for which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.
Claims (5)
1. A method for prolonging the shelf life of Paecilomyces lilacinus by using a double-layer embedding technology is characterized by comprising the following raw materials: paecilomyces lilacinus; water-soluble high molecular material, ultraviolet protective agent, cross-linking agent, zeolite and water; the ultraviolet ray protective agent is one or more of riboflavin, fluorescein and ascorbic acid.
2. The method for prolonging the shelf life of Paecilomyces lilacinus by using the double-layer embedding technology according to claim 1, comprising the following steps:
the method comprises the following steps: mixing a water-soluble polymer material and water according to a mass ratio of 1: 1-50, heating and stirring for 15-30 minutes, and cooling to room temperature to obtain a colloidal solution;
step two: the mass ratio of the colloidal solution to the cross-linking agent is 5-15: 1-5, stirring uniformly to obtain an embedding solution;
step three: uniformly stirring and mixing the paecilomyces lilacinus fermentation liquor and the ultraviolet protective agent according to the mass ratio of 1-5: 0.1-1, uniformly dripping the mixture into embedding liquid at the speed of 30-60 drops/minute, stirring while dripping, and reacting for 5-12 hours to obtain embedded particles;
step four: and uniformly mixing zeolite and the embedded particles, standing for 10-20 hours to obtain outer-layer embedded particles, and exposing in a ventilated and cool environment for 4-10 days to dry in the shade.
3. The method for prolonging the shelf life of the paecilomyces lilacinus by using the double-layer embedding technology as claimed in claim 2, wherein the concentration of the paecilomyces lilacinus is 2.0-50.0 hundred million/ml.
4. The method for prolonging the shelf life of paecilomyces lilacinus by using the double-layer embedding technology according to claims 1-2, wherein the water-soluble high polymer material is any one or more of modified starch, chitosan and sodium alginate agar, and the cross-linking agent is any one of polyacrylamide and calcium salt.
5. The method for prolonging the shelf life of Paecilomyces lilacinus according to claim 4, wherein the calcium salt is selected from calcium carbonate, calcium chloride, and calcium sulfate, and gluconolactone is added to form a slow-release calcium source.
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101100646A (en) * | 2007-06-25 | 2008-01-09 | 中国科学院微生物研究所 | Paecilomyces lilacinus and application thereof |
CN106376602A (en) * | 2016-08-31 | 2017-02-08 | 江西天人生态股份有限公司 | Paecilomyces lilacinus granule and preparation method thereof |
CN111642520A (en) * | 2020-07-16 | 2020-09-11 | 浙江省柑桔研究所 | Paecilomyces lilacinus wettable powder and application thereof |
-
2020
- 2020-10-14 CN CN202011093827.3A patent/CN112111480A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101100646A (en) * | 2007-06-25 | 2008-01-09 | 中国科学院微生物研究所 | Paecilomyces lilacinus and application thereof |
CN106376602A (en) * | 2016-08-31 | 2017-02-08 | 江西天人生态股份有限公司 | Paecilomyces lilacinus granule and preparation method thereof |
CN111642520A (en) * | 2020-07-16 | 2020-09-11 | 浙江省柑桔研究所 | Paecilomyces lilacinus wettable powder and application thereof |
Non-Patent Citations (2)
Title |
---|
史怀;肖马云;刘波;: "淡紫拟青霉的固定化研究", 武夷科学, no. 00 * |
张寅平: "《建筑环境传质学》", 中国建筑工业出版社, pages: 179 - 180 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113699142A (en) * | 2021-08-30 | 2021-11-26 | 南宁汉和生物科技股份有限公司 | Paecilomyces lilacinus embedding method |
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