CN112107612A - Traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as well as preparation method and application thereof - Google Patents

Abstract

The application discloses a traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young and a preparation method and application thereof, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 125-145 parts of chicken embryo, 1-10 parts of astragalus membranaceus, 1-10 parts of liquorice, 1-10 parts of ginseng and 2-10 parts of honeysuckle. The Chinese medicinal composition has the remarkable effects of tonifying qi and nourishing blood, maintaining beauty and keeping young and improving sleep due to the mutual matching of the components, is a medicinal and edible traditional Chinese medicinal material or a traditional Chinese medicinal material capable of being used for health-care food, has long-term administration experience, and has no toxic or side effect.

Description

Traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as well as preparation method and application thereof

Technical Field

The application relates to a traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young, a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines.

Background

With the increase of the living pressure and the acceleration of the living rhythm of people, many people have the phenomena of deficiency of both qi and blood, insomnia and dreaminess, premature senility of skin and the like due to various unhealthy life modes such as over-fatigue, improper diet, staying up all night and the like. If the people do not tonify qi and nourish blood in time, the people can suffer from fatigue, palpitation, insomnia, sallow complexion, low immunity and the like, and are more prone to diseases, so that the normal life of the people is influenced; the sleeping quality also directly influences the physical state of people, the cognitive learning ability of people is reduced due to poor sleeping quality for a long time, the reaction is slow, the energy is not easy to concentrate, the morbidity of diseases such as obesity, hypertension, hyperlipidemia and diabetes is increased, and the health is seriously damaged.

The existing medicines have the effects of benefiting qi, nourishing blood, improving sleep and delaying skin aging.

Disclosure of Invention

In order to solve the problems, the application provides the traditional Chinese medicine composition for tonifying qi and nourishing blood and maintaining beauty and keeping young, and the preparation method and the application thereof, and the invention solves the problem that the effects of tonifying qi and nourishing blood, improving sleep and delaying skin aging in the existing medicine are not obvious.

The application discloses a traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young, which comprises the following raw materials in parts by weight: 125-145 parts of chicken embryo, 1-10 parts of astragalus membranaceus, 1-10 parts of liquorice, 1-10 parts of ginseng and 2-10 parts of honeysuckle.

Preferably, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 137 parts of chicken embryo, 3 parts of astragalus, 4 parts of liquorice, 2 parts of ginseng and 6 parts of honeysuckle.

The traditional Chinese medicine composition has the advantages that the components are matched with each other, the traditional Chinese medicine composition has remarkable effects of tonifying qi and nourishing blood, maintaining beauty and keeping young and improving sleep, all medicinal materials are medicinal and edible or traditional Chinese medicinal materials capable of being used as health-care food, the traditional Chinese medicine composition has long-term medication experience, and no toxic or side effect is caused.

Optionally, the feed comprises the following raw materials in parts by weight: 125-145 parts of chicken embryo, 1-10 parts of astragalus membranaceus, 1-10 parts of liquorice, 1-10 parts of ginseng, 2-10 parts of honeysuckle, 1-10 parts of fructus amomi, 1-10 parts of cinnamon, 1-10 parts of poria cocos and 2-10 parts of Chinese yam.

Optionally, the traditional Chinese medicine also comprises 2-10 parts of rose and 5-20 parts of Chinese date according to parts by weight.

Preferably, the feed comprises the following raw materials in parts by weight: 137 parts of chicken embryo, 3 parts of astragalus membranaceus, 6 parts of liquorice, 2 parts of ginseng, 6 parts of honeysuckle, 3 parts of fructus amomi, 3 parts of cinnamon, 4 parts of poria cocos, 6 parts of Chinese yam, 6 parts of rose and 10 parts of Chinese date.

Optionally, the feed comprises the following raw materials in parts by weight: 125-145 parts of chicken embryo, 1-10 parts of astragalus membranaceus, 5-20 parts of liquorice, 1-10 parts of ginseng, 2-10 parts of honeysuckle, 1-10 parts of fructus amomi, 1-10 parts of cinnamon, 1-10 parts of poria cocos, 5-20 parts of Chinese yam, 5-20 parts of cistanche, 2-10 parts of raspberry and 2-10 parts of wolfberry fruit.

Preferably, the feed comprises the following raw materials in parts by weight: 137 parts of chicken embryo, 3 parts of astragalus membranaceus, 10 parts of liquorice, 2 parts of ginseng, 6 parts of honeysuckle, 3 parts of fructus amomi, 3 parts of cinnamon, 4 parts of poria cocos, 12 parts of Chinese yam, 12 parts of cistanche, 6 parts of raspberry and 6 parts of wolfberry fruit.

Optionally, the feed comprises the following raw materials in parts by weight: 5-15 parts of astragalus membranaceus, 20-30 parts of cistanche, 2-10 parts of fructus amomi, 30-40 parts of liquorice, 7-17 parts of raspberry, 7-17 parts of wolfberry fruits, 20-30 parts of Chinese yams, 5-15 parts of poria cocos, 140-160 parts of chicken embryo, 2-10 parts of ginseng, 2-10 parts of cinnamon, 7-17 parts of honeysuckle, 25-35 parts of mulberries and 10-20 parts of hawthorn.

Optionally, the donkey-hide gelatin food further comprises 2-8 parts of donkey-hide gelatin according to parts by weight.

Optionally, the composition comprises the following components in parts by weight: 6-14 parts of astragalus membranaceus, 22-28 parts of cistanche, 3-9 parts of fructus amomi, 32-38 parts of liquorice, 9-15 parts of raspberry, 9-15 parts of wolfberry, 22-28 parts of Chinese yam, 7-13 parts of poria cocos, 145-160 parts of chicken embryo, 3-9 parts of ginseng, 3-9 parts of cinnamon, 9-15 parts of honeysuckle, 27-33 parts of mulberry and 12-18 parts of hawthorn.

Optionally, the composition comprises the following components in parts by weight: 6-14 parts of astragalus membranaceus, 22-28 parts of cistanche, 3-9 parts of fructus amomi, 32-38 parts of liquorice, 9-15 parts of raspberry, 9-15 parts of wolfberry, 22-28 parts of Chinese yam, 7-13 parts of poria cocos, 145-160 parts of chicken embryo, 3-9 parts of ginseng, 3-9 parts of cinnamon, 9-15 parts of honeysuckle, 27-33 parts of mulberry, 12-18 parts of hawthorn and 2-8 parts of donkey-hide gelatin.

Optionally, the composition comprises the following components in parts by weight: 12 parts of astragalus membranaceus, 28 parts of cistanche, 8 parts of fructus amomi, 38 parts of liquorice, 15 parts of raspberry, 15 parts of wolfberry fruit, 28 parts of Chinese yam, 12 parts of poria cocos, 155 parts of chicken embryo, 8 parts of ginseng, 8 parts of cinnamon, 15 parts of honeysuckle, 32 parts of mulberry and 18 parts of hawthorn.

Optionally, the composition comprises the following components in parts by weight: 10 parts of astragalus membranaceus, 25 parts of cistanche, 6 parts of fructus amomi, 35 parts of liquorice, 12 parts of raspberry, 12 parts of wolfberry fruit, 25 parts of Chinese yam, 10 parts of poria cocos, 150 parts of chicken embryo, 6 parts of ginseng, 6 parts of cinnamon, 12 parts of honeysuckle, 30 parts of mulberry, 15 parts of hawthorn and 5 parts of donkey-hide gelatin.

The donkey-hide gelatin is added in the traditional Chinese medicine composition, so that the parts of other components can be correspondingly reduced without weakening the efficacy of the traditional Chinese medicine composition, and the efficacies of the traditional Chinese medicine composition for tonifying qi and nourishing blood, maintaining beauty and keeping young and improving sleep can be further strengthened and consolidated; meanwhile, the problems of vigorous liver fire, excessive body supplement and the like caused by excessive other components can be avoided; and the preparation cost of the traditional Chinese medicine composition is reduced under the condition of ensuring the drug effect.

Optionally, the application provides a preparation method of the traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young, which comprises the following steps:

1) crushing ginseng into powder and donkey-hide gelatin into blocks for later use;

2) decocting 13 medicinal materials including astragalus, cistanche, fructus amomi, liquorice, raspberry, wolfberry fruit, Chinese yam, poria cocos, chicken embryo, cinnamon, honeysuckle, mulberry and hawthorn in water for 2-3 times, 1-2 hours each time, filtering for 2-3 times, merging filtrate, and concentrating the filtrate at 85-95 ℃ until the relative density is 1.05-1.10 to obtain final filtrate;

3) adding the ginseng powder and the donkey-hide gelatin blocks prepared in the step 1) into the final filtrate obtained in the step 2), uniformly mixing, continuously concentrating the filtrate at 85-95 ℃ until the relative density is 1.25-1.35, adding 240-260 parts by weight of seasoning accessories, uniformly mixing, and filling.

In the application, the ginseng is crushed into powder, and the donkey-hide gelatin is crushed into blocks so as to accelerate the dissolution speed of the ginseng and the donkey-hide gelatin in the filtrate and shorten the preparation time of the traditional Chinese medicine composition; meanwhile, the effects of the ginseng and the donkey-hide gelatin can be exerted more fully, and the materials are utilized more fully.

Preferably, the number of times of decocting with water in step 2) is 2, each time for 1 hour. Preferably, the number of filtrations in step 2) is 2.

Preferably, the concentration in step 2) is carried out at a temperature with a lower limit selected from 85 ℃, 88 ℃, 91 ℃ or 94 ℃ and an upper limit selected from 85 ℃, 88 ℃, 91 ℃ or 94 ℃.

Preferably, the lower limit of the relative density in step 2) is selected from 1.05, 1.07, 1.09 or 1.10 and the upper limit is selected from 1.05, 1.07, 1.09 or 1.10.

Most preferably, the temperature of concentration in step 2) is 90 ℃ and the relative density of the filtrate is 1.08.

Optionally, the flavoring auxiliary material in step 3) is honey, maltose syrup or glucose syrup. Preferably, the flavoring auxiliary in step 3) is maltose syrup.

Preferably, the lower limit of maltose syrup portion is selected from 246, 252, 258 or 260 and the lower limit is selected from 246, 252, 258 or 260.

Most preferably, the maltose syrup in step 3) is 250 parts. The malt syrup used in the application can be used for harmonizing the bitter taste of the traditional Chinese medicine composition, so that the traditional Chinese medicine composition has a better mouthfeel.

Preferably, the concentration in step 3) is carried out at a temperature with a lower limit selected from 85 ℃, 88 ℃, 91 ℃ or 94 ℃ and an upper limit selected from 85 ℃, 88 ℃, 91 ℃ or 94 ℃.

Preferably, the lower limit of the relative density in step 3) is selected from 1.25, 1.28, 1.31 or 1.34 and the upper limit is selected from 1.25, 1.28, 1.31 or 1.34.

Most preferably, the temperature of concentration in step 3) is 90 ℃ and the relative density of the filtrate is 1.30.

The concentration temperature in the step 2) and the step 3) in the application can enable the ginseng powder and the donkey-hide gelatin blocks to be better dissolved in the filtrate, and can ensure that the nutrient components of each component in the filtrate are not damaged; the relative density in the step 2) can enable the ginseng powder and the donkey-hide gelatin blocks to be better dispersed and dissolved in the filtrate, and if the relative density is too high, the ginseng powder and the donkey-hide gelatin blocks can be unevenly dispersed and dissolved, so that the efficacy of the traditional Chinese medicine composition is influenced; the relative density in the step 3) can make the prepared traditional Chinese medicine composition convenient for subsequent processing and production.

Optionally, the fineness of the ginseng powder is 80-120 meshes, and the fineness of the donkey-hide gelatin blocks is 3-7 meshes.

Optionally, the fineness ratio of the ginseng powder to the donkey-hide gelatin blocks is 18-23.

Preferably, the fineness of the ginseng powder has an upper limit selected from 85 meshes, 95 meshes, 105 meshes or 115 meshes and a lower limit selected from 85 meshes, 95 meshes, 105 meshes or 115 meshes. Most preferably, the fineness of the ginseng powder is 100 meshes, and the fineness of the donkey-hide gelatin block is 5 meshes.

Preferably, the fineness ratio of the ginseng powder to the donkey-hide gelatin block is 20. The fineness ratio of the ginseng powder to the donkey-hide gelatin blocks can enable the ginseng powder and the donkey-hide gelatin blocks to achieve the optimal dispersion and dissolution effects in a close time, so that the processing and preparation time is reduced, and the maximum effective content of the components of the traditional Chinese medicine composition can be ensured.

According to another aspect of the present application, there is provided a use of a traditional Chinese medicine composition for benefiting qi and nourishing blood, and maintaining beauty and keeping young, which prepares the prepared traditional Chinese medicine composition into a soft extract, a tablet, a capsule, an oral solution, a granule, a paste, a powder, an emulsion, a chewable matrix, a dietary supplement, a nutritional supplement, a beverage, a health food and a functional food.

Optionally, the application prepares the prepared traditional Chinese medicine composition into an oral solution;

the preparation method comprises the following steps:

1) mixing the plant materials uniformly, and pulverizing into coarse powder with a pulverizer; pulverizing the animal medicinal material chicken embryo into coarse powder by a pulverizer;

2) percolating and refining plant medicinal materials

2.1) adding 60-80% ethanol into the plant medicinal material coarse powder, stirring, fully infiltrating, moistening for 0.8-1.3 hours, loading into a percolation device, then adding 60-80% ethanol to submerge the medicinal materials, wherein the amount of the ethanol is 3-6 times of that of the medicinal materials, and percolating at a flow rate of one drop per second after soaking for 2-4 days and nights;

2.2) collecting the percolate into a concentrator, recovering ethanol until no alcohol smell exists, and concentrating at the temperature of 75-85 ℃ to obtain a plant medicinal material concentrated solution;

2.3) placing the concentrated solution of the plant medicinal materials into a cold precipitation tank, adding purified water while stirring, uniformly stirring, cooling the liquid to 1-4 ℃, preserving heat and standing for 16-20 hours to obtain refined solution of the plant medicinal materials;

2.4) filtering;

3) percolation and refining of animal medicinal materials

3.1) adding 60-80% ethanol into animal medicinal material coarse powder, stirring, fully infiltrating, moistening for 0.8-1.3 hours, loading into a percolation device, then adding 60-80% ethanol to submerge the medicinal materials, wherein the amount of the ethanol is 3-6 times of that of the medicinal materials, and percolating at a flow rate of one drop per second after soaking for 2-4 days and nights;

3.2) collecting the percolate into a concentrator, recovering ethanol until no alcohol smell exists, and concentrating at 75-85 ℃ to obtain an animal medicinal material concentrated solution;

3.3) placing the concentrated solution of the animal medicinal materials into a cold precipitation tank, adding purified water while stirring, uniformly stirring, adding kaolin, fully and uniformly stirring, cooling the liquid to 1-4 ℃, preserving heat and standing for 16-20 hours to obtain refined solution of the animal medicinal materials;

3.4) filtering and clarifying, adding 1-3% of activated carbon, fully and uniformly stirring, boiling for 8-12 minutes, cooling to 45-50 ℃, and filtering and clarifying again;

4) mixing the animal medicine refined liquid and the plant medicine refined liquid, boiling for 8-12 minutes, cooling to 1-4 ℃, preserving heat, standing for 16-20 hours, filtering and clarifying to obtain a mixed refined liquid;

5) weighing a sweetening agent and potassium sorbate, preparing a 0.1-0.3% cardamom oil wine solution, adding purified water, the sweetening agent and the potassium sorbate into the mixed refined solution, stirring until the mixture is melted, then dripping the cardamom oil wine solution, stirring uniformly, and supplementing the purified water;

6) filtering the feed liquid obtained in the step 5), and refrigerating and storing filtrate;

7) and (6) filling and sterilizing.

Preferably, the application prepares the prepared traditional Chinese medicine composition into an oral solution;

the preparation method comprises the following steps:

1) mixing radix astragali, Cistanchis herba, fructus Amomi, Glycyrrhrizae radix, Rubi fructus, fructus Lycii, rhizoma Dioscoreae, Poria, ovum gallus Domesticus crusta, cortex Cinnamomi, flos Lonicerae, Mori fructus, and fructus crataegi uniformly, and pulverizing into coarse powder with pulverizer; pulverizing the animal medicinal material chicken embryo into coarse powder by a pulverizer;

2) percolating and refining plant medicinal materials

2.1) adding 70% ethanol into the coarse powder of the plant medicinal material, stirring, fully infiltrating, moistening for 1 hour, loading into a percolation device, then adding 70% ethanol to submerge the medicinal material, wherein the amount of the ethanol is 5 times of that of the medicinal material, and percolating at a flow rate of one drop per second after soaking for 3 days and nights;

2.2) collecting the percolate into a concentrator, recovering ethanol until no alcohol smell exists, and concentrating at 80 ℃ to obtain a plant medicinal material concentrated solution;

2.3) placing the concentrated solution of the plant medicinal materials into a cold precipitation tank, adding purified water while stirring, uniformly stirring, cooling the liquid to 3 ℃, preserving heat and standing for 18 hours to obtain refined solution of the plant medicinal materials;

2.4) filtering;

3) percolation and refining of animal medicinal materials

3.1) adding ethanol with the concentration of 70% into the animal medicinal material coarse powder, stirring, fully infiltrating, moistening for 1 hour, filling into a percolation device, then adding ethanol with the concentration of 70% to submerge the medicinal materials, wherein the amount of the ethanol is 5 times of that of the medicinal materials, and percolating at the flow rate of one drop per second after soaking for 3 days and nights;

3.2) collecting the percolate into a concentrator, recovering ethanol until no alcohol smell exists, and concentrating at 80 ℃ to obtain an animal medicinal material concentrated solution;

3.3) placing the concentrated solution of the animal medicinal materials into a cold precipitation tank, adding purified water while stirring, uniformly stirring, adding kaolin, fully and uniformly stirring, cooling the liquid to 3 ℃, preserving heat and standing for 18 hours to obtain refined solution of the animal medicinal materials;

3.4) filtering and clarifying, adding active carbon with the mass fraction of 2 percent of the refined liquid, fully and uniformly stirring, boiling for 10 minutes, cooling to 48 ℃, filtering and clarifying again;

4) mixing the refined liquid of animal medicinal materials and the refined liquid of plant medicinal materials, boiling for 10 min, cooling to 3 deg.C, standing for 18 hr, filtering, and clarifying to obtain mixed refined liquid;

5) weighing sweetener and potassium sorbate, preparing 0.2% cardamom oil wine solution, adding purified water, sweetener and potassium sorbate into the mixed refined solution, stirring to melt, adding the cardamom oil wine solution dropwise, stirring, and supplementing purified water;

6) filtering the feed liquid obtained in the step 5), and refrigerating and storing filtrate;

7) and (6) filling and sterilizing.

Optionally, the medicinal materials are radix astragali, herba cistanches, fructus Amomi, radix Glycyrrhizae, fructus Rubi, fructus Lycii, rhizoma Dioscoreae, Poria, embryo gallus domesticus, cortex Cinnamomi, flos Lonicerae, fructus Mori, fructus crataegi, colla Corii Asini, radix Ginseng, flos Rosae Rugosae, and fructus Jujubae.

The effects of the medicinal materials in the application are as follows:

astragalus root: has effects of invigorating qi, consolidating exterior, promoting urination, expelling toxin, expelling pus, healing sore, and promoting granulation. Modern pharmacological research shows that astragalus has the effects of improving immunity, enhancing oxidation resistance, resisting myocardial ischemia, eliminating free radicals, resisting tumors, resisting aging and the like.

Cistanche deserticola: modern pharmacology shows that the cistanche has a plurality of pharmacological actions of regulating immune activity, resisting aging, improving learning and memory ability, protecting nerves, resisting viruses and tumors, influencing intestinal flora and the like.

Amomum fruit: resolving dampness, promoting appetite, warming spleen, relieving diarrhea, resisting ulcer, resisting inflammation, resisting blood platelet aggregation, and prolonging blood coagulation time.

Raspberry: the health-care tea has the effects of tonifying kidney, controlling nocturnal emission, reducing urination, nourishing liver and improving eyesight, and has obvious activity in the aspects of resisting tumors, resisting aging, removing free radicals and the like as effective components of terpenes, flavones, alkaloids, coumarins and the like.

Wolfberry fruit: nourishing liver and kidney, clearing away heat, improving eyesight, enhancing immunity, resisting aging, tumor, stress and blood sugar, reducing blood lipid, protecting eye, and resisting oxygen free radical injury.

Chinese yam: the Chinese medicinal composition has the effects of invigorating spleen, tonifying lung, reinforcing kidney and replenishing vital essence, and has the biological activities of resisting tumor, regulating immunity, resisting oxidation, resisting diabetes, resisting mutation and the like.

Tuckahoe, poria cocos: has effects in nourishing brain, strengthening body constitution, invigorating spleen, regulating stomach function, increasing myocardial blood flow, promoting urination, protecting liver, relieving inflammation, resisting oxidation, promoting immunity, and delaying aging.

Chicken embryo: the chicken embryo contains rich amino acids, lecithin, unsaturated fatty acid, calcium, zinc, iron, vitamins and other nutrient components, and has the functions of strengthening body constitution, nourishing brain, tranquilizing, resisting senility, tonifying deficiency and nourishing vitality.

Ginseng: has effects of invigorating primordial qi, recovering pulse, relieving depletion, invigorating spleen, benefiting lung, promoting fluid production, nourishing blood, tranquilizing mind, and improving intelligence. The main components are saccharides, saponins, proteins, polypeptides and the like, and the health-care food has the pharmacological effects of improving memory and immunity, improving cardiovascular and cerebrovascular diseases, delaying aging, reducing blood sugar, resisting tumors and the like.

Cinnamon: has effects in tonifying yang, warming kidney, dispelling cold, dredging collaterals, relieving pain, resisting gastric ulcer, resisting inflammation, resisting bacteria, resisting tumor, tranquilizing mind, relieving spasm, and relieving fever, and contains polysaccharides, polyphenols, coumarin and inorganic elements.

Honeysuckle flower: clearing away heat and toxic material, eliminating swelling and dispersing carbuncle, and has the functions of resisting pathogenic microbe, resisting inflammation, resisting virus, clearing away heat, reducing blood fat, etc.

And (3) mulberry fruit: as a food with homology of medicine and food, contains effective components such as anthocyanin, polysaccharide, resveratrol, water-soluble protein and the like, and has pharmacological effects of reducing blood sugar, resisting oxidation, protecting immunity, recovering motor function, protecting kidney, protecting spleen, inhibiting bacteria, resisting inflammation and the like.

Hawthorn fruit: the hawthorn has the effects of strengthening spleen, stimulating appetite, promoting digestion, removing food stagnation, promoting blood circulation, reducing phlegm, expelling tapeworm, treating hernia, promoting growth and the like, and modern researches find that the hawthorn can reduce blood pressure, reduce blood fat, resist atherosclerosis, arrhythmia, myocardial ischemia, inhibit brain cell apoptosis and the like when being applied to a cardiovascular system and a cerebrovascular system.

Licorice root: invigorating spleen and qi, clearing away heat and toxic materials, moistening lung, arresting cough, and harmonizing the above materials.

E, donkey-hide gelatin: is a good tonic, and is especially a holy product for women, which can beautify skin, enrich blood and tonify qi. It has the following main effects: 1. for nourishing yin and tonifying blood, it is combined with E jiao to nourish yin and tonify blood. 2. Nourishing yin and moistening dryness, and can be used for treating cough due to yin deficiency and hyperactivity of heart fire, lung deficiency with heat, and yin and blood deficiency. 3. The product is a good product for nourishing skin and maintaining beauty. 4. Blood nourishing and hemostasis, pulmonary tuberculosis hemoptysis, thrombocytopenic hemorrhage, etc. 5. Regulating menstruation and preventing miscarriage, so it can nourish fetus, prevent miscarriage and regulate and treat pregnancy.

Chinese date: has sweet and warm nature and taste, and has effects of invigorating stomach, invigorating spleen, replenishing blood, nourishing, and strengthening body constitution. Enhancing immunity, protecting liver, lowering blood pressure, and protecting heart. Beautifying, whitening and removing freckles. Delaying senility. To invigorate qi and nourish blood.

And (3) rose: bitter and cold; has little toxicity. Enter lung and large intestine meridians, clear away heat and toxic material, and dispel dampness. Has effects of warming and nourishing blood vessels, warming stomach and nourishing stomach, relieving gastrointestinal nerve, moistening skin, removing black speck, improving skin color, and can be used for adjuvant treatment of female dysmenorrhea, menoxenia, etc. Has effects in promoting wound healing, promoting digestion, eliminating fat, and caring skin.

Benefits that can be produced by the present application include, but are not limited to:

1. the traditional Chinese medicine composition for tonifying qi, nourishing blood, maintaining beauty and keeping young, provided by the application, has the remarkable effects of tonifying qi, nourishing blood, maintaining beauty and keeping young and improving sleep by mutually matching the components, is a traditional Chinese medicine which can be used as both medicine and food or can be used for health food, has long-term medication experience, and has no toxic or side effect.

2. The traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young, provided by the application, has no bitter and astringent taste or peculiar smell of traditional Chinese medicine materials, has good taste and is easily accepted by people taking medicines.

3. The traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young, provided by the application, has the advantages that the concentration temperature in the processing and preparation process can enable the ginseng powder and the donkey-hide gelatin blocks to be better dissolved in the filtrate, and the nutrient components of all components in the filtrate can be guaranteed not to be damaged; the relative density in the preparation step 2) can enable the ginseng powder and the donkey-hide gelatin blocks to be better dispersed and dissolved in the filtrate, and if the relative density is too high, the ginseng powder and the donkey-hide gelatin blocks are not uniformly dispersed and dissolved, so that the efficacy of the traditional Chinese medicine composition is influenced.

4. According to the traditional Chinese medicine composition for tonifying qi, nourishing blood, maintaining beauty and keeping young, the fineness ratio of the ginseng powder to the donkey-hide gelatin blocks can enable the ginseng powder and the donkey-hide gelatin blocks to achieve the optimal dispersing and dissolving effects in a similar time, the processing and preparation time is shortened, and the maximum effective content of the components of the traditional Chinese medicine composition can be guaranteed.

Detailed Description

The present application will be described in detail with reference to examples, but the present application is not limited to these examples.

Unless otherwise specified, the raw materials and reagents in the examples of the present application were all purchased commercially.

Example 1

Preparation of composition No. 1

1) Pulverizing 6 parts of Ginseng radix into powder with fineness of 100 mesh, crushing 5 parts of colla Corii Asini into blocks with fineness of 5 mesh, and the fineness ratio of Ginseng radix powder and colla Corii Asini blocks is 20;

2) decocting 13 kinds of medicinal materials including 10 parts of astragalus membranaceus, 25 parts of cistanche, 6 parts of fructus amomi, 35 parts of liquorice, 12 parts of raspberry, 12 parts of wolfberry fruit, 25 parts of Chinese yam, 10 parts of poria cocos, 150 parts of chicken embryo, 6 parts of cinnamon, 12 parts of honeysuckle, 30 parts of mulberry and 15 parts of hawthorn in water for 2 times, filtering for 2 times each time for 1 hour, combining the filtrates, and concentrating the filtrate at 90 ℃ until the relative density is 1.08 to obtain the final filtrate;

3) adding the ginseng powder and the donkey-hide gelatin blocks prepared in the step 1) into the final filtrate obtained in the step 2), uniformly mixing, continuously concentrating the filtrate at 90 ℃ until the relative density is 1.30, adding 250 parts of malt syrup according to the parts by weight, uniformly mixing, and filling.

According to the parts of the medicinal materials in the composition 1#, the compositions 1# to 3# and the comparative compositions 1# to 5# are respectively prepared, and the difference of the preparation method and the preparation method of the composition 1# is shown in Table 1.

TABLE 1

Compositions 1# to 3# and comparative compositions 1# to 5# were characterized for efficacy as shown in table 2. The characteristics are divided into two parts, one part is sensory index, and the other part is physicochemical index. Sensory indicators include taste; physical and chemical indexes: (1) the effective components in the traditional Chinese medicine comprise alkaloid and other substances, and the content of total alkaloid in the traditional Chinese medicine composition is measured by an ultraviolet spectrophotometry; (2) the viscosity of the Chinese medicinal composition is measured by a rotational viscometer.

TABLE 2

Serial number	Total alkaloid content (%)	Viscosity (cP)	Taste of the product
				1#	28	10080	No bitter and astringent taste, slight sweet
2#	25	9520	No bitter and astringent taste, slight sweet
				3#	23	11300	No bitter and astringent taste, slight sweet
Comparative composition No. 1	8	10004	No bitter and astringent taste, slight sweet
				Comparative composition No. 2	22	4708	No bitter and astringent taste, slight sweet
Comparative composition No. 3	26	9984	Has little fishy smell and is sweet and greasy
				Comparative composition No. 4#	18	9458	No bitter and astringent tasteSlightly sweet
Comparative composition No. 5	11	8840	Has solid granular feeling and is slightly sweet

The comparison of the composition No. 1 to No. 3 shows that the traditional Chinese medicine composition prepared according to the parameter range of the preparation method provided by the application has no obvious difference in the overall components and the characterization parameters, and can achieve the required effect.

As can be seen from the comparison between the composition 1# and the comparative composition 1#, the concentration temperature in the step 2) and the step 3) can affect the content of the effective components of the prepared traditional Chinese medicine composition, and the alkaloid in the traditional Chinese medicine composition can be damaged when the concentration temperature is too high, so that the effective content in the traditional Chinese medicine composition is obviously reduced.

As can be seen from the comparison between the composition No. 1 and the comparative composition No. 2, the setting of the relative density finally affects the viscosity of the prepared traditional Chinese medicine composition, and the traditional Chinese medicine composition is too thin due to too low viscosity and difficult to be processed into different dosage forms subsequently.

As can be seen by comparing the composition No. 1 with the comparative composition No. 3, the addition of the maltose syrup with the same parts can harmonize the taste of the prepared traditional Chinese medicine composition, and the honey can make the taste of the traditional Chinese medicine composition sweet and greasy without completely eliminating fishy smell.

As can be seen from composition No. 1, comparative composition No. 4 and comparative composition No. 5, if the mesh number of the ginseng powder and the donkey-hide gelatin block is larger, the content of the total alkaloids in the traditional Chinese medicine composition is reduced after heating and concentrating; if the mesh number of the ginseng powder and the donkey-hide gelatin block is smaller, the ginseng powder and the donkey-hide gelatin block cannot be completely melted in the heating concentration process, so that the content of total alkaloids in the final traditional Chinese medicine composition is greatly reduced, the taste is not good, obvious granular sensation exists, and in the preparation process, if the fineness ratio of the ginseng powder to the donkey-hide gelatin block is not in a proper range, the dispersibility of the ginseng powder and the donkey-hide gelatin block is poor, and the taste and the production quality of the product are influenced.

Example 2

The preparation method of the other traditional Chinese medicine composition comprises the following steps:

1) mixing the plant medicinal materials uniformly, and crushing the mixture into plant medicinal material coarse powder by a crusher; pulverizing the animal medicinal material chicken embryo into animal medicinal material coarse powder by a pulverizer;

2) percolating and refining plant medicinal materials

2.1) adding 60-80% ethanol into the coarse powder of the plant medicinal material, stirring, fully infiltrating, moistening for 0.8-1.3 hours, filling into a percolation device, then adding 60-80% ethanol to submerge the medicinal material, wherein the amount of the ethanol is 3-6 times of that of the medicinal material, and percolating at a flow rate of one drop per second after soaking for 2-4 days;

2.2) collecting the percolate into a concentrator, recovering ethanol until no alcohol smell exists, and concentrating at the temperature of 75-85 ℃ to obtain a plant medicinal material concentrated solution;

2.3) placing the concentrated solution of the plant medicinal materials into a cold precipitation tank, adding purified water while stirring, uniformly stirring, cooling the liquid to 1-4 ℃, preserving heat and standing for 16-20 hours to obtain refined solution of the plant medicinal materials;

2.4) filtering;

3) percolation and refining of animal medicinal materials

3.1) adding 60-80% ethanol into animal crude powder, stirring, fully infiltrating, moistening for 0.8-1.3 hours, loading into a percolation device, then adding 60-80% ethanol to submerge the crude powder, wherein the amount of the ethanol is 3-6 times of that of the crude powder, and percolating at a flow rate of one drop per second after soaking for 2-4 days;

3.2) collecting the percolate into a concentrator, recovering ethanol until no alcohol smell exists, and concentrating at 75-85 ℃ to obtain an animal medicinal material concentrated solution;

3.3) placing the concentrated solution of the animal medicinal materials into a cold precipitation tank, adding purified water while stirring, uniformly stirring, adding kaolin, fully and uniformly stirring, cooling the liquid to 1-4 ℃, preserving heat and standing for 16-20 hours to obtain refined solution of the animal medicinal materials;

3.4) filtering and clarifying, adding 1-3% of activated carbon, fully and uniformly stirring, boiling for 8-12 minutes, cooling to 45-50 ℃, and filtering and clarifying again;

4) mixing the animal medicine refined liquid and the plant medicine refined liquid, boiling for 8-12 minutes, cooling to 1-4 ℃, preserving heat, standing for 16-20 hours, filtering and clarifying to obtain a mixed refined liquid;

5) weighing a sweetening agent and potassium sorbate, preparing a 0.1-0.3% cardamom oil wine solution, adding purified water, the sweetening agent and the potassium sorbate into the mixed refined solution, stirring until the mixture is melted, then dripping the cardamom oil wine solution, stirring uniformly, and supplementing the purified water;

6) filtering the feed liquid obtained in the step 5), and refrigerating and storing filtrate;

7) and (6) filling and sterilizing.

It is to be understood that the preparation method of example 1 in the present application is generally used for preparing a paste, and the preparation method of example 2 is generally used for preparing an oral liquid.

Example 3

Samples # 1 to # 15 and comparative sample # 1 were prepared by either of the above-described preparation methods of example 1 or example 2, and the differences in formulation from sample # 1 are shown in table 3.

TABLE 3

Example 4 characterization of the mouse experiment

The drugs used in the following experimental sample No. 1 to sample No. 15 and comparative sample No. 1 are all sample No. 1 to sample No. 15 and comparative sample No. 1 prepared in example 3.

Experiment one: qi and blood deficiency mouse experiment

1. Modeling

The method is characterized in that a ' tail-cutting bloodletting and intraperitoneal injection of cyclophosphamide ' of a mouse ' is adopted for molding, NIH male mice are 18-22 g and are fed adaptively for 3 days, 20 mice in each group are randomly grouped according to weight, namely a blank control group, a model control group, a sample 1# group-sample 15# group, a comparison sample 1# group and a positive control group, the drug amount of each group is 10g/kg, and the positive control group adopts commercially available compound donkey-hide gelatin syrup. The mice of each group are administered by intragastric administration, the model control group and the blank control group are administered by intragastric administration of physiological saline with the same amount, the intragastric administration volume is 20ml/kg, 1 time/day, and the administration is continuously carried out for 10 days. On the 4 th, 6 th and 8 th days after the first administration, 0.25ml/10g of tail vein bleeding of the model group and the administration group, on the 5 th, 7 th and 9 th days, 40mg/kg of cyclophosphamide is injected into the abdominal cavity of mice of the model group and the administration groups, and 30mg/kg of normal saline is injected into the abdominal cavity of mice of the blank group, so that the animal model with deficiency of both qi and blood is prepared.

2. Experimental methods

After the administration group is administered for 1 hour for the last time, randomly selecting 10 mice per group, carrying out eyeball blood collection, and measuring Red Blood Cells (RBC), hemoglobin (Hb) and Platelets (PLT) by an automatic whole blood analyzer after blood collection; at the same time, serum is separated and collected according to a conventional method, and the contents of Erythropoietin (EPO), interleukin-2 (IL-2) and interleukin-3 (IL-3) in the serum are measured by an enzyme-linked immunosorbent assay.

After 1h of the last gavage of the dosing group, the remaining mice of each group were subjected to a bar-rotating test. The function coordinated movement of the mouse is observed at 16r/min through a rotating rod type fatigue instrument, and the residence time of the mouse on the rotating rod within 3min is recorded. If the mouse did not drop throughout the procedure, the mouse rod-rotating residence time was 180 s.

The tail of each group of mice is a certain weight (5 percent of the weight) and placed in a smooth swimming cylinder with the inner wall at the water temperature of 25-30 ℃ for swimming, and the control group and the administration group are alternately carried out. The stopwatch records the time from the beginning of swimming after the mouse is put into water to death, namely the swimming time.

The data analysis adopts a statistical method, SPSS 16.0 software is used for processing the obtained data, One-way ANOVA is selected for statistical analysis, and the data is analyzed by

And (4) showing. Two by two comparisons with T test, with P<0.05 is a significant difference, P<0.01 is a very significant difference, which has statistical significance.

3. Results of the experiment

The control group mice have bright hair color, normal appetite and defecation and flexible action; the hair color of the mice in the model group is dull and easy to fall off, anorexia, loose and wet stool, dislike activity, crouch, arch back and somnolence; the mouse hair color, appetite, defecation condition and activity of the sample 1# to 15# group and the comparative sample 1# group are obviously improved compared with the model group, the mice of the sample 1# to 15# group have no obvious difference, the mice of the comparative sample 1# group have certain improvement compared with the model group, but the effect of the mice of the comparative sample 1# group is obvious without the sample 1# to 15# group, and the effects of the mice of the sample 4# group, the sample 5# group and the sample 7# to 15# group without donkey-hide gelatin are poorer than those of the mice of other donkey-hide gelatin added sample groups.

Compared with a blank control group, the WBC, RBC, Hb and PLT contents of the mice in the model control group are all obviously reduced (P is less than 0.01), and the success of modeling is shown; compared with a model group, the sample 1# to 15# and the positive drug group can obviously increase Hb and PLT contents (P is less than 0.01), the comparative sample 1# has an increasing effect, but is not as obvious as other sample groups, and the sample 4# group, the sample 5# group and the sample 7# to 15# group which are not added with donkey-hide gelatin increase Hb and the PLT contents are poorer than other sample groups; the sample No. 1-15 group and the positive control group can obviously increase the WBC content (P <0.01 or P <0.05), the comparative sample No. 1 group has the increasing effect, but is not as obvious as other sample groups, and the sample No. 4, sample No. 5 and sample No. 7-15 groups which are not added with the donkey-hide glue have lower WBC content than other sample groups; the sample 1# to 15# group and the positive group can significantly increase the RBC content (P <0.01 or P <0.05), and the comparative sample 1# group has no significant difference (P > 0.05). The WBC, RBC and Hb levels of the sample 1# group, the sample 2# group, the sample 3# group, the sample 6# group and the positive group were not significantly different compared with the normal group, and were close to the normal group level, and the results are shown in table 4.

TABLE 4 data of WBC, RBC, Hb, PLT for each group of mice with deficiency of both Qi and blood

Note: p <0.05, P < 0.01; compared to the blank group, # P <0.05, # P < 0.01.

Compared with a blank control group, the contents of EPO and IL-3 in the model control group are obviously reduced (P is less than 0.01), and the content of IL-2 is obviously increased (P is less than 0.01); compared with the model group, the samples 1# to 15# can obviously increase the content of EPO (P <0.01 or P <0.05), the samples 1# group, 2# group, 3# group, 4# group, 5# group, 6# group, 8# group, 11# group, 12# group, 13# group, 14# group, 15# group and the positive control group can obviously reduce the content of IL-2 (P <0.01 or P <0.05), the samples 7# group, 9# group, 10# group and the comparative sample 1# group have the tendency of reducing IL-2, but the difference is not significant (P >0.05), the IL-3 content can be increased significantly in the group of sample No. 1, the group of sample No. 2, the group of sample No. 3 and the positive control group (P <0.01 or P <0.05), and the groups of sample No. 4-15 and the group of comparative sample No. 1 have increasing tendency but have no significant difference (P > 0.05). Compared with the blank group, the EPO, IL-2 and IL-3 contents of the sample No. 1 group and the sample No. 2 group have no significant difference (P is more than 0.05), and the results are shown in the table 5.

TABLE 5 data of groups of mice with deficiency of both qi and blood EPO, IL-2, IL-3

Note: p <0.05, P < 0.01; compared to the blank group, # P <0.05, # P < 0.01.

Compared with a blank control group, the time of the model control group mouse on a rotating rod and the swimming time are obviously reduced, which indicates that the model is successfully made (P is less than 0.01); the sample 1# group, sample 2# group, sample 3# group, sample 4# group, sample 5# group, sample 6# group, sample 8# group, sample 11# group, sample 14# group and positive control group significantly increased the mouse rotarod movement and swimming movement time (P <0.01 or P <0.05) compared to the model group. Compared with the blank group, the swimming time of the sample No. 1 group, the sample No. 2 group, the sample No. 3 group and the sample No. 6 group is close to the level of the blank group, no significant difference (P >0.05) exists, and the swimming time of the sample No. 4 group, the sample No. 5 group and the sample No. 7-15 group which are not added with the donkey-hide gelatin are slightly shorter than those of other sample groups on a rotating rod. The results are shown in Table 6.

TABLE 6 data of the time of the rod-rotating exercise and swimming exercise for each group of mice with deficiency of both qi and blood

Group of	Dosage (g/kg)	Time on rotating bar(s)	Swimming time(s)
				Blank control group	0	151.00±16.23**	311.00±42.89**
Model control group	0	92.51±14.74##	214.56±84.00##
				Positive control group	10	119.54±13.82**##	299.54±35.81**
Sample No. 1 group	10	125.11±24.86**##	305.11±37.59**
				Sample No. 2 group	10	120.91±28.99**##	299.91±41.55**
Sample No. 3 group	10	119.56±25.66**##	300.51±48.77**
				Sample No. 4 group	10	105.32±28.22*##	280.56±54.55*#
Sample No. 5 group	10	104.89±21.35*##	280.95±58.33*#
				Sample No. 6 group	10	118.98±28.45**##	301.10±58.21**
Sample 7# group	10	99.18±22.14##	248.25±50.21##
				Sample No. 8 group	10	104.51±17.12*##	279.81±49.54*#
Sample No. 9 group	10	99.62±24.71##	260.48±39.61#
				Sample No. 10 group	10	99.68±20.37##	251.29±42.47#
Sample No. 11 group	10	104.72±18.20*##	280.34±50.21*#
				Sample No. 12 group	10	100.01±22.31##	264.05±42.18*#
Sample No. 13 group	10	100.42±20.12##	273.17±42.15*#
				Sample No. 14 group	10	107.49±25.21*##	285.61±44.01*
Sample No. 15 group	10	101.95±19.55##	274.61±39.64*#
				Comparative sample No. 1 group	10	100.56±28.85##	243.51±59.43##

The results show that the samples 1# to 15# all obviously inhibit the reduction of RBC, WBC, Hb and PLT caused by deficiency of both qi and blood; the rod rotating time and the load swimming time of the mouse can be prolonged to different degrees; obviously increases the EPO content in the serum of the mouse, increases the IL-3 content in the serum of the mouse and reduces the IL-2 content in the serum of the mouse in different degrees. The comparative sample # 1 group using the comparative sample # 1 with the changed number of parts had some effect, but the effect was not significant, compared to the comparative sample # 1 group. The group 4, 5 and 7# to 15# samples, to which no donkey-hide gelatin was added, were slightly less effective than the other groups. The result shows that the traditional Chinese medicine composition prepared by the application can improve general physical signs of mice with deficiency of both qi and blood, and has remarkable effects of resisting deficiency of both qi and blood and resisting fatigue.

Experiment two: mouse experiment for delaying skin aging

1. Modeling

A model of mice aging is induced by combining D-galactose and ultraviolet irradiation. Selecting Kunming mice which are female and have the weight of 18-22 g, and randomly grouping: sample # 1 to sample # 15, control sample # 1, model control, blank control and positive control. The back of the mice had been depilated by about 5cm × 5cm, and the group except the blank group was given 1% D-galactose 1ml/100g subcutaneously to the neck and back daily, and subjected to UVB ultraviolet irradiation for 30 min/day for 42 days to create a subacute aging model, and the blank group was given the same dose of physiological saline. The traditional Chinese medicine composition prepared by intragastric administration of the model group, the sample No. 1 group, the sample No. 15 group and the comparison sample No. 1 group, and the vitamin E solution for intragastric administration of the positive group is 50mg/kg once a day for 42 days continuously.

2. Experimental methods

After each administration group is administered for 1h, blood is taken from eye sockets of mice, centrifuged, and the supernatant is taken to measure the content of superoxide dismutase (SOD) and Malondialdehyde (MDA). Removing back hair, shearing back skin, defatting, homogenizing tissue, and collecting supernatant to detect SOD and MDA content.

The data analysis adopts a statistical method, SPSS 16.0 software is used for processing the obtained data, One-way ANOVA is selected for statistical analysis, and the data is analyzed by

And (4) showing. Two by two comparisons with T test, with P<0.05 is a significant difference, P<0.01 is a very significant difference, which has statistical significance.

3. Principle of experiment

D-galactose is injected subcutaneously, and the galactitol which is a metabolite of the D-galactose cannot be further metabolized by cells and is accumulated in the cells, so that the normal osmotic pressure is influenced, the cells are swollen, and simultaneously, a large amount of free radicals generated by the oxidation of the D-galactose in vivo exceed the scavenging capacity of a body, so that the lipid peroxidation is initiated to cause the degenerative change and the functional change of the animal tissue cells during aging, and researches show that the aging degree of the model is close to the 21-month age level of mice, and the changes are consistent with the natural aging change. Ultraviolet irradiation can affect the growth, metabolism and other functions of fibroblast cells in the dermis, and belongs to an exogenous factor of aging molding.

SOD is the main antioxidant enzyme for eliminating free radicals of the organism, can reduce the generation of oxygen free radicals and lipid peroxide, the oxygen free radicals are increased along with the increase of the age, and meanwhile, if the activity of SOD in the body is reduced, the oxygen free radicals are accumulated, so that the organism is quickly aged; therefore, the activity of serum and skin SOD is closely related to aging, and can indirectly reflect the capability of organism to remove oxygen free radicals. The MDA which is the final product of the decomposition of the lipid peroxide is an extremely active cross-linking agent and can be cross-linked with protein, nucleic acid and the like so as to denature and inactivate the lipid peroxide, and the cross-linked substances are accumulated in cells to form lipofuscin and enable the skin to have color spots and the like, so the MDA content can indirectly reflect the degree of damage of tissue cells by free radicals.

Whether the skin is kept smooth and tender mainly depends on the collagen under the dermis, Hyp is the amino acid with the highest content and the most stable in the collagen, is obtained by hydroxylating proline and accounts for about 13% of the total amount of the collagen amino acid, when the skin is aged, the crosslinking of collagen fibers and other macromolecules is increased, and under the action of free radicals, the macromolecular crosslinking is generated to reduce the activity of the hydroxylamidoyl hydroxylase, so that the hydroxylation of the proline is weakened, the content of hydroxyproline is reduced, and the synthesis of the collagen is further influenced. So that its content reflects the degree of aging of the animal skin.

4. Results of the experiment

The test result shows that the SOD content of the D-galactose aging model is obviously reduced (P is less than 0.01) compared with that of the blank group, and the MDA content is obviously increased (P is less than 0.01), which indicates that the molding is successful. The positive control group and the 16 sample groups can effectively reverse the D-galactose induced change, and compared with the model group, the mouse serum SOD activity of the sample No. 1 to 15 and the mouse serum SOD activity of the comparative sample No. 1 are obviously improved (P <0.01), and the MDA content is obviously reduced, (P <0.01) wherein the sample No. 1 group, the sample No. 2 group, the sample No. 3 group and the sample No. 6 group are close to the normal group level (P >0.05), and the table 7 shows.

TABLE 7 SOD activity and MDA content in serum of each group of mice

The test result shows that the D-galactose effectively replicates a mouse model of skin aging, and the SOD activity, the Hyp content and the MDA content of the skin of the mouse model are all significantly different from those of a blank control group (P < 0.01). Compared with the model group, the positive control group and the samples 1# to 15# can obviously improve the activity of SOD and the skin moisture percentage in the skin tissues of mice (P <0.01 and P <0.05) and obviously reduce the MDA content (P <0.01), and the samples 1# group, 2# group, 3# group, 4# group, 5# group, 6# group, 8# group, 10# group, 11# group, 12# group, 13# group, 14# group and 15# group can obviously improve the Hyp content (P <0.01 and P < 0.05). The sample 7# group, the sample 9# group, and the comparative sample 1# group had some effect, which was not as significant as the other sample groups. The group 4, 5 and 7# to 15# samples, to which no donkey-hide gelatin was added, were slightly less effective than the other groups. Wherein the level of the sample No. 1 group is close to that of the positive control group, the difference is less significant, the SOD activity and the skin water content of the sample No. 1 group are close to that of the blank group (P >0.05), and no significant difference exists, as shown in Table 8.

TABLE 8 SOD activity, MDA and Hyp content in skin tissue of mice in each group

The results show that the SOD activity and the MDA content of the serum and the skin tissues of the mice aged by the D-galactose can be obviously improved and reduced by each administration group, which indicates that the traditional Chinese medicine composition prepared by the application can improve the activity of antioxidant enzyme and reduce the generation of lipid peroxide. The experimental result shows that the Hyp content of the skin tissue of mice in each administration group is increased in different degrees through the intervention of the traditional Chinese medicine composition, so that the synthesis of skin collagen is promoted, and the effects of enhancing the skin elasticity and delaying the skin aging are prompted.

The samples prepared by the method have strong antioxidant bioactivity, and the aging of skin is obviously delayed by the sample 1# group to the sample 15# group. The comparative sample No. 1 group with the modified formulation had some effect but the effect was not significant compared to the comparative sample No. 1 group. The group of sample 4#, sample 5#, sample 7#, sample 8#, sample 9#, sample 10#, sample 11#, sample 12#, sample 13#, sample 14# and sample 15# to which no donkey-hide gelatin was added were slightly less effective than the other sample groups. The traditional Chinese medicine composition prepared by the application has a remarkable effect of delaying skin aging.

Experiment three: mouse experiment for improving sleep

1. Modeling

An ICR mouse is selected to research the sleep improvement effect of the traditional Chinese medicine composition prepared by the application. ICR mice were female, weighing 20 ± 2g, and after 7 days of acclimation, were randomly grouped: the positive control group comprises 10 samples from the 1# group to the 15# group, a comparison sample from the 1# group and the blank control group, the 1# group to the 15# group and the 1# group are orally administrated with gastric lavage respectively, the gastric lavage amount is 20ml/kg, the continuous administration is carried out for 28 days, the positive control group is perfused with the traditional Chinese medicine tea for aiding sleep with the same volume, and the blank control group is perfused with the distilled water with the same volume.

2. Experimental methods

(1) General behavioral observations

The behavior state of the mice is observed every day, the mental state and behavior of the mice in the administration group are not obviously different from those of the control group, and the hair color of the mice in the administration group with different dosages is glossy compared with that of the control group. Before and after the start of the experiment, the mice were weighed to observe the change in body weight. The weight data are subjected to t-test by using SPSS software, the results are shown in Table 9, and the weights of mice in different dose administration groups and control groups have no significant difference (P is more than 0.05), namely, the traditional Chinese medicine composition prepared by the application has no significant influence on the weights of the mice, which shows that the traditional Chinese medicine composition in the dose of the application has no adverse influence on the weights of the mice. TABLE 9 body weight results of mice before and after the experiment

(2) Direct sleep experiment

After each administration group was last administered for 30min, the control group and each administration group were observed for sleep. The sleep takes the disappearance of righting reflex as an index, if the righting reflex is not righted after exceeding 60s, the righting reaction is disappeared, and the mouse enters the sleep. The result shows that the mice of the blank control group and the administration group do not have direct sleep after the mice are administered with the test drug for 30min, which indicates that the traditional Chinese medicine composition prepared by the application has no direct sleep effect.

(3) Experiment for prolonging sleep time of sodium pentobarbital

After each administration group is administered for 15min at the last time, the intraperitoneal injection of 50mg/kg of pentobarbital sodium is performed on mice of each group, the injection amount is 0.1ml/10g, the starting and ending time of the disappearance of the positive reflex is taken as an index, and whether the test medicament can prolong the sleep time induced by the pentobarbital sodium is observed. The result shows that under the hypnotic effect of 50mg/kg sodium pentobarbital, the sleep time of the mice in each administration group is greatly prolonged (P <0.01) compared with that of a blank control group, the sleep time of the mice in a positive control group is remarkably prolonged (P <0.05) compared with that of the blank control group, but the sleep time of the mice in each administration group is shorter; the group of sample No. 1, the group of sample No. 2, the group of sample No. 3, the group of sample No. 6 and the group of sample No. 14 have very significant differences ((P <0.01) compared with the positive control group, and the group of sample No. 4, the group of sample No. 5, the group of sample No. 7, the group of sample No. 8, the group of sample No. 9, the group of sample No. 10, the group of sample No. 11, the group of sample No. 12, the group of sample No. 13, the group of sample No. 15 and the group of comparative sample No. 1 have no significant differences (P >0.05) compared with the positive control group.

TABLE 10 sleep time of mice in each group

Note: "+" indicates experimental groups compared to blank control groups,. + -. P <0.05,. + -. P < 0.01; "#" indicates that # P <0.05 and # P <0.01 in the experimental group compared with the positive group.

(4) Pentobarbital sodium subthreshold dose hypnosis experiment

Before the experiment begins, a subthreshold dose investigation experiment of the sodium pentobarbital is carried out, namely the maximum dose of the sodium pentobarbital which does not disappear in 80-90% of mouse positive reaction. After administration for 15min, 30mg/kg of sodium pentobarbital is intraperitoneally injected into each group of mice, and the injection amount is 0.1ml/10 g. The standard of falling asleep is 60s when the mouse positive reflection disappears. And recording the sleep number of the mice within 30min, and observing whether the sleep incidence rate of animals with the sub-threshold dose of the sodium pentobarbital can be improved by samples with different formulas of each administration group. As a result, as shown in table 11, it was found that the amount of the mice falling asleep was increased in each of the administration groups compared to the blank control group, and the group to which the sample # 1 prepared in the best number of administration parts was administered was the best in the effect of falling asleep.

TABLE 11 sleep volume of mice in each group

Group of	Dosage (g/kg/day)	Number of animals sleeping (one)	Ratio of sleeping animals (%)
				Blank control group	0	1	10
Positive control group	10	2	20
				Sample No. 1 group	10	7	70
Sample No. 2 group	10	5	50
				Sample No. 3 group	10	4	40
Sample No. 4 group	10	3	30
				Sample No. 5 group	10	3	30
Sample No. 6 group	10	4	40
				Sample 7# group	10	1	10
Sample No. 8 group	10	3	30
				Sample No. 9 group	10	2	20
Sample No. 10 group	10	2	20
				Sample No. 11 group	10	3	30
Sample No. 12 group	10	2	20
				Sample No. 13 group	10	3	30
Sample No. 14 group	10	4	40
				Sample No. 15 group	10	3	30
Comparative sample No. 1 group	10	3	30

(5) Influence of traditional Chinese medicine composition and sleep-aiding traditional Chinese medicine tea on sleep latency of sodium pentobarbital

After 15min of the last administration, 240mg/kg of sodium pentobarbital is injected into the abdominal cavity of each group of mice, and the injection amount is 0.1ml/10 g. The sleep latencies of the groups with different dosages and the blank control group are recorded, and the difference of the sleep latencies of different groups is compared, so that the sleep latency of each administration group can be obviously shortened (P is less than 0.01) compared with that of the blank control group, and the sleep latency of the sample No. 1 group is the shortest. The results are shown in Table 12.

TABLE 12 sleep latency in groups of mice

Group of	Dosage (g/kg/d)	Sleep latency (min)
			Blank control group	0	18.58±0.84
Positive control group	10	17.36±0.94*
			Sample No. 1 group	10	13.36±0.92**##
Sample No. 2 group	10	14.04±0.86**##
			Sample No. 3 group	10	14.53±0.88**##
Sample No. 4 group	10	15.35±0.97**##
			Sample No. 5 group	10	15.30±0.95**##
Sample No. 6 group	10	14.61±0.91**##
			Sample 7# group	10	17.25±0.69*
Sample No. 8 group	10	16.24±0.93**#
			Sample No. 9 group	10	17.07±0.79*
Sample No. 10 group	10	16.21±0.84**#
			Sample No. 11 group	10	15.51±0.82**##
Sample No. 12 group	10	16.01±0.88**#
			Sample No. 13 group	10	15.95±0.97**#
Sample No. 14 group	10	15.25±0.94**##
			Sample No. 15 group	10	15.87±0.89**#
Comparative sample No. 1 group	10	16.24±0.88**#

Note: "+" indicates experimental groups compared to blank control groups,. + -. P <0.05,. + -. P < 0.01; "#" indicates that # P <0.05 and # P <0.01 in the experimental group compared with the positive group.

According to experimental results, the traditional Chinese medicine composition prepared by the application can obviously improve the hair color of a tested mouse, so that the tested mouse is smoother and glossy, has no adverse effect on the weight of the tested mouse, and has no direct sleeping effect on the mouse. Can prolong the sleep time induced by the sodium pentobarbital, increase the sub-threshold dose of the sodium pentobarbital to induce the sleep rate of animals, and shorten the sleep latency period of the sodium pentobarbital to induce the animals to sleep. The effect of the comparative sample No. 1 is inferior to that of the samples No. 1 to No. 15, and the effects of the samples No. 4, No. 5, No. 7, No. 8, No. 9, No. 10, No. 11, No. 12, No. 13, No. 14 and No. 15 without donkey-hide gelatin are slightly inferior to those of the other samples, and the effect of the commercial sleep-aiding herbal tea is less significant than that of the samples No. 1 to No. 15.

The embodiments in the present specification are described in a progressive manner, and the same and similar parts among the embodiments are referred to each other, and each embodiment focuses on the differences from the other embodiments. In particular, for the system embodiment, since it is substantially similar to the method embodiment, the description is simple, and for the relevant points, reference may be made to the partial description of the method embodiment.

The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims (10)

1. The traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young is characterized by comprising the following raw materials in parts by weight: 125-145 parts of chicken embryo, 1-10 parts of astragalus membranaceus, 1-10 parts of liquorice, 1-10 parts of ginseng and 2-10 parts of honeysuckle.

2. The traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as claimed in claim 1, is characterized by comprising the following raw materials in parts by weight: 125-145 parts of chicken embryo, 1-10 parts of astragalus membranaceus, 1-10 parts of liquorice, 1-10 parts of ginseng, 2-10 parts of honeysuckle, 1-10 parts of fructus amomi, 1-10 parts of cinnamon, 1-10 parts of poria cocos and 2-10 parts of Chinese yam.

3. The traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as claimed in claim 2, which is characterized by further comprising 2-10 parts of rose and 5-20 parts of Chinese date according to parts by weight.

4. The traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as claimed in claim 1, is characterized by comprising the following raw materials in parts by weight: 125-145 parts of chicken embryo, 1-10 parts of astragalus membranaceus, 5-20 parts of liquorice, 1-10 parts of ginseng, 2-10 parts of honeysuckle, 1-10 parts of fructus amomi, 1-10 parts of cinnamon, 1-10 parts of poria cocos, 5-20 parts of Chinese yam, 5-20 parts of cistanche, 2-10 parts of raspberry and 2-10 parts of wolfberry fruit.

5. The traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as claimed in claim 1, is characterized by comprising the following raw materials in parts by weight: 5-15 parts of astragalus membranaceus, 20-30 parts of cistanche, 2-10 parts of fructus amomi, 30-40 parts of liquorice, 7-17 parts of raspberry, 7-17 parts of wolfberry fruits, 20-30 parts of Chinese yams, 5-15 parts of poria cocos, 140-160 parts of chicken embryo, 2-10 parts of ginseng, 2-10 parts of cinnamon, 7-17 parts of honeysuckle, 25-35 parts of mulberries and 10-20 parts of hawthorn.

6. The traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as claimed in claim 5, characterized by further comprising 2-8 parts of donkey-hide gelatin by weight.

7. The preparation method of the traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as claimed in claim 6, which is characterized by comprising the following steps:

1) crushing ginseng into powder and donkey-hide gelatin into blocks for later use;

2) decocting 13 medicinal materials including astragalus, cistanche, fructus amomi, liquorice, raspberry, wolfberry fruit, Chinese yam, poria cocos, chicken embryo, cinnamon, honeysuckle, mulberry and hawthorn in water for 2-3 times, 1-2 hours each time, filtering for 2-3 times, merging filtrate, and concentrating the filtrate at 85-95 ℃ until the relative density is 1.05-1.10 to obtain final filtrate;

3) adding the ginseng powder and the donkey-hide gelatin blocks prepared in the step 1) into the final filtrate obtained in the step 2), uniformly mixing, continuously concentrating the filtrate at 85-95 ℃ until the relative density is 1.25-1.35, adding 240-260 parts by weight of seasoning accessories, uniformly mixing, and filling.

8. The preparation method of the traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as claimed in claim 7, wherein the fineness of the ginseng powder is 80-120 meshes, and the fineness of the donkey-hide gelatin block is 3-7 meshes.

9. The application of the traditional Chinese medicine composition for benefiting qi, nourishing blood and maintaining beauty is characterized in that the prepared traditional Chinese medicine composition is prepared into soft extract, tablets, capsules, oral solution, granules, paste, powder, emulsion, chewable matrix, dietary supplement, nutritional supplement, beverage, health food and functional food.

10. The application of the traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young according to claim 9 is characterized in that the prepared traditional Chinese medicine composition is prepared into an oral solution;

the preparation method comprises the following steps:

1) mixing the plant medicinal materials uniformly, and crushing the mixture into plant medicinal material coarse powder by a crusher; pulverizing the animal medicinal material chicken embryo into animal medicinal material coarse powder by a pulverizer;

2) percolating and refining plant medicinal materials

2.1) adding 60-80% ethanol into the coarse powder of the plant medicinal material, stirring, fully infiltrating, moistening for 0.8-1.3 hours, filling into a percolation device, then adding 60-80% ethanol to submerge the medicinal material, wherein the amount of the ethanol is 3-6 times of that of the medicinal material, and percolating at a flow rate of one drop per second after soaking for 2-4 days;

2.2) collecting the percolate into a concentrator, recovering ethanol until no alcohol smell exists, and concentrating at the temperature of 75-85 ℃ to obtain a plant medicinal material concentrated solution;

2.3) placing the concentrated solution of the plant medicinal materials into a cold precipitation tank, adding purified water while stirring, uniformly stirring, cooling the liquid to 1-4 ℃, preserving heat and standing for 16-20 hours to obtain refined solution of the plant medicinal materials;

2.4) filtering;

3) percolation and refining of animal medicinal materials

3.1) adding 60-80% ethanol into animal crude powder, stirring, fully infiltrating, moistening for 0.8-1.3 hours, loading into a percolation device, then adding 60-80% ethanol to submerge the crude powder, wherein the amount of the ethanol is 3-6 times of that of the crude powder, and percolating at a flow rate of one drop per second after soaking for 2-4 days;

3.2) collecting the percolate into a concentrator, recovering ethanol until no alcohol smell exists, and concentrating at 75-85 ℃ to obtain an animal medicinal material concentrated solution;

3.3) placing the concentrated solution of the animal medicinal materials into a cold precipitation tank, adding purified water while stirring, uniformly stirring, adding kaolin, fully and uniformly stirring, cooling the liquid to 1-4 ℃, preserving heat and standing for 16-20 hours to obtain refined solution of the animal medicinal materials;

3.4) filtering and clarifying, adding 1-3% of activated carbon, fully and uniformly stirring, boiling for 8-12 minutes, cooling to 45-50 ℃, and filtering and clarifying again;

4) mixing the animal medicine refined liquid and the plant medicine refined liquid, boiling for 8-12 minutes, cooling to 1-4 ℃, preserving heat, standing for 16-20 hours, filtering and clarifying to obtain a mixed refined liquid;

5) weighing a sweetening agent and potassium sorbate, preparing a 0.1-0.3% cardamom oil wine solution, adding purified water, the sweetening agent and the potassium sorbate into the mixed refined solution, stirring until the mixture is melted, then dripping the cardamom oil wine solution, stirring uniformly, and supplementing the purified water;

6) filtering the feed liquid obtained in the step 5), and refrigerating and storing filtrate;

7) and (6) filling and sterilizing.