CN115715793A - Herbal paste containing chicken embryo and rhizoma polygonati and production method thereof - Google Patents
Herbal paste containing chicken embryo and rhizoma polygonati and production method thereof Download PDFInfo
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Abstract
The invention relates to a chicken embryo rhizoma polygonati herbal paste and a production method thereof, wherein the paste comprises the following components: the traditional Chinese medicine composition is prepared from chicken embryo, rhizoma polygonati, wolfberry fruit, mulberry, raspberry, chinese yam, poria cocos, liquorice, donkey-hide gelatin, longan pulp, chinese date, dried ginger, peach kernel and double red rose by the following method: pulverizing colla Corii Asini, and melting to obtain colla Corii Asini solution; dynamically extracting the rest materials with water vapor to obtain extractive solution, concentrating under reduced pressure to obtain concentrated solution, adding activated carbon, heating, filtering, adding molten colla Corii Asini solution into the filtrate, concentrating under normal pressure, packaging, and sterilizing to obtain the final product. The production process is optimized and screened, the process is scientific and stable, and the quality is controllable; rat experiments prove that the product has obvious effects of tonifying qi and blood and protecting female reproductive systems, has no obvious toxic or side effect, is safe to take and is an effective oral product; has the effects of nourishing blood, removing blood stasis, warming channels, relieving pain, warming yang and dispelling cold.
Description
Technical Field
The invention relates to the technical field of medicines, in particular to a chicken embryo rhizoma polygonati herbal paste suitable for solving the problems of female cold uterus, poor postpartum recovery and the like and a preparation method thereof.
Background
At present, the problems of cold womb, poor postpartum recovery and the like of women exist in a large quantity, although some formulas are disclosed to be conditioned by medicines such as medlar, mulberry, raspberry, donkey-hide gelatin, dried longan pulp, medlar, jujube, dried ginger and the like, wherein the medlar, the mulberry and the raspberry can tonify kidney, nourish blood and replenish essence; colla Corii Asini, arillus longan, fructus Lycii, and fructus Jujubae can nourish blood, flos Rosae Rugosae and semen Persicae can regulate qi-flowing, promote blood circulation, and prevent and treat blood stasis, rhizoma Polygonati, rhizoma Dioscoreae, poria, glycyrrhrizae radix, and fructus Jujubae can invigorate spleen and qi, and Zingiberis rhizoma can warm yang and dispel cold; however, the effect of the traditional Chinese medicine is not obvious when the traditional Chinese medicine is used alone, but for the research of compound preparations, the traditional Chinese medicine is few, and a few compound preparations are simple in formula, single in dosage form and low in bioavailability; meanwhile, due to the particularity of postpartum women, the medicine has quite strict requirements, and many traditional Chinese medicines cannot be used for the puerpera due to the side effect on infants, so that the compound effect advantage of the traditional Chinese medicine compound preparation is difficult to realize.
The market urgently needs to research a product which is prepared from raw materials with homology of medicine and food, has small side effect, accords with the traditional Chinese medicine theory and can be taken for a long time.
Disclosure of Invention
In order to overcome the defects, the invention aims to provide herbal paste for chicken fetal jaundice and a production method thereof.
The technical scheme adopted by the invention for solving the technical problem is as follows: a chicken embryo yolk essence herbal paste comprises the following components: 50-70 parts of chicken embryo, 70-80 parts of rhizoma polygonati, 40-60 parts of wolfberry fruit, 90-110 parts of mulberry, 40-60 parts of raspberry, 70-80 parts of Chinese yam, 140-160 parts of poria cocos, 20-40 parts of liquorice, 1-10 parts of donkey-hide gelatin, 70-80 parts of longan aril, 140-160 parts of Chinese date, 40-60 parts of dried ginger, 50-70 parts of peach kernel and 40-60 parts of double-petal red rose.
The preparation method comprises the following steps: pulverizing colla Corii Asini, and melting to obtain colla Corii Asini solution; dynamically extracting the rest materials with water vapor to obtain extractive solution, concentrating under reduced pressure to obtain concentrated solution, adding activated carbon, heating, filtering, adding molten colla Corii Asini solution into the filtrate, concentrating under normal pressure, packaging, and sterilizing to obtain the final product.
The specific operation method comprises the following steps:
step 1, taking a proper amount of donkey-hide gelatin, crushing, taking water with the amount of 3-8 times that of the donkey-hide gelatin, boiling, adding donkey-hide gelatin powder, and stirring to dissolve;
step 2, decocting: putting the rest medicinal materials in a multifunctional extraction tank, adding 6-10 times of drinking water, decocting for 2 hr, filtering, adding 5-8 times of drinking water, decocting for 2 hr, and filtering;
the decocting steam pressure is 0.1-0.3Mpa, and the time for decocting starts to be timed when the liquid medicine is boiling;
step 3, double-effect concentration: mixing the decoctions, concentrating in a double-effect concentrator until the relative density is 1.10-1.20 (measured at 70-90 deg.C);
the control parameters in the concentration process are as follows:
steam pressure: <0.25Mpa;
vacuum degree: the first effect vacuum is controlled to be-0.02 to-0.04 Mpa, and the second effect vacuum is controlled to be-0.04 to-0.06 Mpa;
evaporation temperature: the first effective temperature is controlled at 50-95 ℃;
controlling the secondary effect temperature at 45-85 ℃;
step 4, adding 2-5% of activated medicinal active carbon into the concentrated solution, fully stirring, sucking into a settling tank, boiling for 10-20 minutes, cooling to 45-50 ℃, and filtering to be clear;
step 5, adding the donkey-hide gelatin solution while the donkey-hide gelatin solution is hot, and continuously concentrating the donkey-hide gelatin solution to the required relative density which is between 1.20 and 1.40;
step 6, washing bottles: putting the glass bottles meeting the regulations into a bottle washing machine for washing bottles, observing the water level in the water storage tank at any time, and supplementing at any time;
the bottle washing process control parameters are as follows:
purified water pressure: 0.2-0.3MPa;
compressed air pressure: 0.3-0.4MPa;
circulating water pressure: 0.2-0.3MPa;
water temperature for washing bottles: 50-60 ℃;
drying the cleaned glass bottle, adjusting the equipment parameters to 230 ℃ and drying;
step 7, filling
After the empty bottles are sterilized and dried, the empty bottles are conveyed to a bottle arranging track of a filling machine through a conveying belt, bottle caps are prepared, and automatic filling is carried out;
step 8, sterilization
And (5) sterilizing the filled glass bottles in a sterilization cabinet. And (3) sterilization temperature: 105-120 ℃; and (3) sterilization time: 20-30 minutes;
and 9, checking and packaging.
The product is prepared by concentrating Chinese medicinal materials by vacuum concentration. The reduced pressure can lower the boiling point of water, so that water can be evaporated at a lower temperature, and thus, the evaporation of water can be reduced, and the volatilization of alkaloid can be reduced. The concentration efficiency under reduced pressure is higher than that under normal pressure.
The product uses a plate-and-frame filter to filter the liquid medicine in the production process. The plate-frame filter is applied to pressurized closed filtration, so that the loss of filtrate is less, the filtration quality is good, and the efficiency is high.
The invention has the beneficial effects that: the production process is optimized and screened, the process is scientific and stable, and the quality is controllable; rat experiments prove that the product has obvious effects of tonifying qi and blood and protecting female reproductive systems, has no obvious toxic or side effect, is safe to take and is an effective oral product; has the effects of nourishing blood, removing blood stasis, warming channels, relieving pain, warming yang and dispelling cold; can effectively improve the symptoms of deficiency of both qi and blood, intolerance of cold, little menstrual flow, endless lochia after childbirth, lower abdomen psychroalgia and the like; can be used for treating asthenia, hypoimmunity, deficiency of qi and blood, anemia, bad complexion, ovary function reduction, puerperal uterine involution and so on; has the conditioning effect on lusterless complexion, sallow complexion, scanty menstruation, blood clots, periodic disorder, poor sleep quality, dreaminess and the like caused by deficiency of both spleen and kidney, deficiency of both qi and blood and the like.
Concrete real-time mode
The present invention will now be described in further detail.
Example one
A chicken embryo yolk essence herbal paste comprises the following components: 50Kg of chicken embryo, 70Kg of rhizoma polygonati, 50Kg of fructus lycii, 90Kg of mulberry, 50Kg of raspberry, 75Kg of yam, 150Kg of poria cocos, 30Kg of liquorice, 1Kg of donkey-hide gelatin, 70Kg of arillus longan, 140Kg of jujube, 50Kg of dried ginger, 50Kg of peach kernel and 40Kg of heavy-petal rose flower.
The preparation method comprises the following steps: pulverizing colla Corii Asini, and melting to obtain colla Corii Asini solution; dynamically extracting the rest materials with water vapor to obtain extractive solution, concentrating under reduced pressure to obtain concentrated solution, adding activated carbon, heating, filtering, concentrating under normal pressure, adding melted colla Corii Asini solution, packaging, and sterilizing to obtain the final product.
The specific operation method comprises the following steps:
step 1, taking a proper amount of donkey-hide gelatin, crushing, taking water with the amount of 3 times that of the donkey-hide gelatin, boiling, adding donkey-hide gelatin powder, and stirring to dissolve;
step 2, decocting: putting the rest medicinal materials into a multifunctional extraction tank, adding the medicinal materials into 8 times of drinking water, decocting for 2 hours, filtering, adding the medicinal materials into 6 times of drinking water, decocting for 2 hours, and filtering;
decocting under 0.1Mpa steam pressure; the time of decoction is counted when the liquid medicine is boiling.
Step 3, double-effect concentration: mixing the decoctions, concentrating in a double-effect concentrator until the relative density is 1.1-1.2 (measured at 70-90 deg.C), to obtain concentrated solution with weight: 670kg (adjusted according to the weight of the concentrate and the ratio of the raw materials (1-1.3));
the control parameters in the concentration process are as follows:
steam pressure: <0.25Mpa;
vacuum degree: the first effect vacuum is controlled to be-0.02 to-0.04 Mpa, and the second effect vacuum is controlled to be-0.04 to-0.06 Mpa;
evaporation temperature: the first effective temperature is controlled at 50-95 ℃;
controlling the secondary effect temperature at 45-85 ℃;
step 4, adding 2% of activated medicinal active carbon into the concentrated solution, fully stirring, sucking into a settling tank, boiling for 10 minutes, cooling to 45-50 ℃, and filtering to be clear;
step 5, adding the donkey-hide gelatin solution while the solution is hot, and continuously concentrating the solution to the required density;
step 6, washing bottles: putting the glass bottles meeting the regulations into a bottle washing machine for washing bottles, observing the water level in the water storage tank at any time, and supplementing at any time;
the bottle washing process control parameters are as follows:
purified water pressure: 0.2-0.3MPa;
compressed air pressure: 0.3-0.4MPa;
circulating water pressure: 0.2-0.3MPa;
water temperature for washing bottles: 50 ℃;
drying the cleaned glass bottle, adjusting the equipment parameters to 230 ℃ and drying;
step 7, filling
After the empty bottles are sterilized and dried, the empty bottles are conveyed to a bottle arranging track of a filling machine through a conveying belt, bottle caps are prepared, and automatic filling is carried out;
step 8, sterilization
And (5) sterilizing the filled glass bottles in a sterilization cabinet. Sterilization temperature: 105-120 ℃; and (3) sterilization time: 20 minutes;
and 9, checking and packaging.
Example two
A chicken embryo yolk essence herbal paste comprises the following components: 60Kg of chicken embryo, 75Kg of rhizoma polygonati, 60Kg of medlar, 100Kg of mulberry, 60Kg of raspberry, 70Kg of yam, 140Kg of tuckahoe, 20Kg of liquorice, 3Kg of donkey-hide gelatin, 80Kg of longan aril, 150Kg of jujube, 40Kg of dried ginger, 70Kg of peach kernel and 50Kg of heavy petal rose.
The preparation method comprises the following steps: pulverizing colla Corii Asini, and melting to obtain colla Corii Asini solution; dynamically extracting the rest materials with water vapor to obtain extractive solution, concentrating under reduced pressure to obtain concentrated solution, adding activated carbon, heating, filtering, concentrating under normal pressure, adding melted colla Corii Asini solution, packaging, and sterilizing to obtain the final product.
The specific operation method comprises the following steps:
step 1, taking a proper amount of donkey-hide gelatin, crushing, taking water with the amount of 5 times that of the donkey-hide gelatin, boiling, adding donkey-hide gelatin powder, and stirring to dissolve;
step 2, decocting: placing the rest medicinal materials in a multifunctional extraction tank, adding 6 times of medicinal materials, decocting with water for 2 hr, filtering, adding 5 times of medicinal materials, decocting with drinking water for 2 hr, and filtering;
decocting under 0.2Mpa steam pressure; the time of decoction is counted when the liquid medicine is boiling.
Step 3, double-effect concentration: mixing the decoctions, concentrating in a double-effect concentrator until the relative density is 1.1-1.2 (measured at 70-90 deg.C), to obtain concentrated solution with weight: 810kg (adjusted according to the weight of the concentrate and the ratio of the raw materials (1-1.3));
the control parameters in the concentration process are as follows:
steam pressure: <0.25Mpa;
vacuum degree: the first effect vacuum is controlled to be-0.02 to-0.04 Mpa, and the second effect vacuum is controlled to be-0.04 to-0.06 Mpa;
evaporation temperature: the first effective temperature is controlled at 50-95 ℃;
controlling the secondary effect temperature at 45-85 ℃;
step 4, adding 3% of activated medicinal active carbon into the concentrated solution, fully stirring, sucking into a settling tank, boiling for 20 minutes, cooling to 45-50 ℃, and filtering to be clear;
step 5, adding the donkey-hide gelatin solution while the donkey-hide gelatin solution is hot, and continuously concentrating the donkey-hide gelatin solution to the required density;
step 6, washing bottles: putting the glass bottles meeting the regulations into a bottle washing machine for washing bottles, observing the water level in the water storage tank at any time, and supplementing at any time;
the bottle washing process control parameters are as follows:
purified water pressure: 0.2-0.3MPa;
compressed air pressure: 0.3-0.4MPa;
circulating water pressure: 0.2-0.3MPa;
water temperature for washing bottles: at 55 ℃;
drying the cleaned glass bottle, adjusting the equipment parameters to 230 ℃ and drying;
step 7, filling
Conveying the sterilized and dried empty bottles to a bottle arranging rail of a filling machine through a conveying belt, preparing bottle caps and automatically filling;
step 8, sterilization
And (5) sterilizing the filled glass bottles in a sterilization cabinet. And (3) sterilization temperature: 105-120 ℃; and (3) sterilization time: 30 minutes;
and 9, checking and packaging.
EXAMPLE III
A chicken embryo yolk essence herbal paste comprises the following components: 70Kg of chicken embryo, 80Kg of rhizoma polygonati, 40Kg of medlar, 110Kg of mulberry, 40Kg of raspberry, 80Kg of yam, 160Kg of tuckahoe, 40Kg of liquorice, 10Kg of donkey-hide gelatin, 75Kg of longan aril, 160Kg of jujube, 60Kg of dried ginger, 60Kg of peach kernel, and 60Kg of rose with heavy petals.
The preparation method comprises the following steps: pulverizing colla Corii Asini, and melting to obtain colla Corii Asini solution; dynamically extracting the rest materials with water vapor to obtain extractive solution, concentrating under reduced pressure to obtain concentrated solution, adding activated carbon, heating, filtering, concentrating under normal pressure, adding melted colla Corii Asini solution, packaging, and sterilizing to obtain the final product.
The specific operation method comprises the following steps:
step 1, taking a proper amount of donkey-hide gelatin, crushing, taking water with the amount 6 times that of the donkey-hide gelatin, boiling, adding donkey-hide gelatin powder, and stirring to dissolve;
step 2, decocting: placing the rest materials in a multifunctional extraction tank, adding the materials in 7 times of drinking water, decocting for 2 hr, filtering, adding the materials in 1000L of 8 times of drinking water, decocting for 2 hr, and filtering;
decocting under 0.3Mpa steam pressure; the time of decoction is counted when the liquid medicine is boiling.
Step 3, double-effect concentration: mixing the decoctions, concentrating in a double-effect concentrator until the relative density is 1.1-1.2 (measured at 70-90 deg.C), to obtain concentrated solution with weight: 950kg (adjusted according to the weight of the concentrate and the ratio of the raw materials (1-1.3));
the control parameters in the concentration process are as follows:
steam pressure: <0.25Mpa;
vacuum degree: the first effect vacuum is controlled to be-0.02 to-0.04 Mpa, and the second effect vacuum is controlled to be-0.04 to-0.06 Mpa;
evaporation temperature: the primary effect temperature is controlled at 50-95 ℃;
controlling the secondary effect temperature at 45-85 ℃;
step 4, adding 4% of activated medicinal active carbon into the concentrated solution, fully stirring, sucking into a settling tank, boiling for 15 minutes, cooling to 45-50 ℃, and filtering to be clear;
step 5, adding the donkey-hide gelatin solution while the solution is hot, and continuously concentrating the solution to the required density;
step 6, washing bottles: putting the glass bottles meeting the regulations into a bottle washing machine for washing bottles, observing the water level in the water storage tank at any time, and supplementing at any time;
the bottle washing process control parameters are as follows:
purified water pressure: 0.2-0.3MPa;
compressed air pressure: 0.3-0.4MPa;
circulating water pressure: 0.2-0.3MPa;
the water temperature of the washing bottle is 60 ℃;
drying the cleaned glass bottle, adjusting the equipment parameters to 230 ℃ and drying;
step 7, filling
After the empty bottles are sterilized and dried, the empty bottles are conveyed to a bottle arranging track of a filling machine through a conveying belt, bottle caps are prepared, and automatic filling is carried out;
step 8, sterilization
And (3) sterilizing the filled glass bottles in a sterilization cabinet, wherein the sterilization temperature is as follows: 105-120 ℃; and (3) sterilization time: 25 minutes;
and 9, checking and packaging.
Example four
A chicken embryo yolk essence herbal paste comprises the following components: 60Kg of chicken embryo, 75Kg of rhizoma polygonati, 50Kg of fructus lycii, 100Kg of mulberry, 50Kg of raspberry, 75Kg of yam, 150Kg of poria cocos, 30Kg of liquorice, 5Kg of donkey-hide gelatin, 75Kg of arillus longan, 150Kg of jujube, 50Kg of dried ginger, 60Kg of peach kernel and 50Kg of heavy-petal rose flower.
The preparation method comprises the following steps: pulverizing colla Corii Asini, and melting to obtain colla Corii Asini solution; dynamically extracting the rest materials with water vapor to obtain extractive solution, concentrating under reduced pressure to obtain concentrated solution, adding activated carbon, heating, filtering, concentrating under normal pressure, adding melted colla Corii Asini solution, packaging, and sterilizing to obtain the final product.
The specific operation method comprises the following steps:
step 1, taking a proper amount of donkey-hide gelatin, crushing, taking water with the amount of 8 times of that of the donkey-hide gelatin, boiling, adding the donkey-hide gelatin powder, and stirring to dissolve;
step 2, decocting: putting the rest medicinal materials into a multifunctional extraction tank, adding 10 times of drinking water, decocting for 2 hr, filtering, adding 7 times of drinking water, decocting for 2 hr, and filtering;
decocting under 0.2Mpa steam pressure; the time of decoction is counted when the liquid medicine is boiling.
Step 3, double-effect concentration: mixing the decoctions, concentrating in a double-effect concentrator until the relative density is 1.1-1.2 (measured at 70-90 deg.C), to obtain concentrated solution with weight: 980kg (adjusted according to the weight of the concentrate and the ratio of the raw materials (1-1.3));
the control parameters in the concentration process are as follows:
steam pressure: <0.25Mpa;
vacuum degree: the first effect vacuum is controlled to be-0.02 to-0.04 Mpa, and the second effect vacuum is controlled to be-0.04 to-0.06 Mpa;
evaporation temperature: the first effective temperature is controlled at 50-95 ℃;
controlling the secondary effect temperature at 45-85 ℃;
step 4, adding 5% of activated medicinal active carbon into the concentrated solution, fully stirring, sucking into a settling tank, boiling for 15 minutes, cooling to 45-50 ℃, and filtering to be clear;
step 5, adding the donkey-hide gelatin solution while the solution is hot, and continuously concentrating the solution to the required density;
step 6, washing bottles: putting the glass bottles meeting the regulations into a bottle washing machine for washing bottles, observing the water level in the water storage tank at any time, and supplementing at any time;
the bottle washing process control parameters are as follows:
purified water pressure: 0.2-0.3MPa;
compressed air pressure: 0.3-0.4MPa;
circulating water pressure: 0.2-0.3MPa;
the bottle washing water temperature is 55 ℃;
drying the cleaned glass bottle, adjusting the equipment parameters to 230 ℃ and drying;
step 7, filling
After the empty bottles are sterilized and dried, the empty bottles are conveyed to a bottle arranging track of a filling machine through a conveying belt, bottle caps are prepared, and automatic filling is carried out;
step 8, sterilization
And (3) sterilizing the filled glass bottle in a sterilization cabinet, wherein the sterilization temperature is as follows: 105-120 ℃; and (3) sterilization time: 25 minutes;
and 9, checking and packaging.
The specific experiment is shown below
1. Action on anemia
1.1 action experiment of rhizoma Polygonati herbal paste of chicken embryo on hemorrhagic anemia
50 healthy male KM mice with the weight of 18-22g are selected and pre-fed for 3d, 10 mice are taken out to be used as blank control groups, and the rest 40 mice are molded. Wiping mouse tail with 75% alcohol cotton ball to dilate blood vessel, cutting off 0.2-0.3cm tip of mouse tail, and soaking wound of mouse tail in graduated test tube filled with water at 37 + -1 deg.C until the mouse loses blood 0.5ml to obtain model group. After 24 hours of blood loss, the patients are randomly divided into 4 groups, 10 patients are in each group, and the groups are respectively a model control group, a hen embryo sealwort herb paste 2, 1, 0.5g/kg high, medium and low dosage groups. Each group of mice was gavaged with the corresponding drug solution 20ml/kg, and the blank and model control groups were given pure water of equal volume once a day for 14 days continuously and fasted for 18 hours before the last administration. Mice were observed daily for changes in mental, appetite, fur, eyes, tail and body weight since the start of dosing. After 1 hour of the last administration, blood was collected from the abdominal aorta after anesthesia, and blood indicators such as Red Blood Cells (RBC), hemoglobin (HGB), and Hematocrit (HCT) were measured for each group of mice by a hematology analyzer. The results are shown below:
1.1.1 Effect on morphology in mice model for hemorrhagic anemia
The blank control group mice had normal activity; the fur has luster; the nose and lips are clean, moist and light pink; the waist and the back are straight, and the appetite is normal. The model group mice show listlessness, tiredness, little lying, sleepiness and loose and lusterless fur; pale and lusterless lips; the arch back is contracted in groups, and the weight is obviously reduced; the appetite decreases and the drinking water increases. After the mice in the three dose groups of the polygonatum kingianum herbal paste are administrated, compared with a model control group, the mice are lightened in different degrees, the state is obviously better than that of the model group, and the improvement degree has obvious dose dependence.
1.1.2 Effect on blood cell count in mice model for hemorrhagic anemia
Compared with a blank control group mouse, the model group mouse has the advantages that RBC, HGB and HCT are obviously reduced, and the success of model building of the hemorrhagic anemia model is proved. After 14 days of administration, RBC, HGB and HCT of the mice in the hen embryo sealwort herb paste 2 and 1g/kg dose groups are obviously increased (P is less than 0.05), and RBC, HGB and HCT in the blood of the mice in the 0.5g/kg dose groups are also increased, but have no statistical significance. The fact that the polygonatum sibiricum herbal paste has a remarkable effect on blood loss anemia at a dosage of more than 1g/kg is shown in table 1.1.
TABLE 1.1 Effect of herbal paste of Polygonatum Canaliculatum on blood cell count in model mice with hemorrhagic anemia: (
n＝10)
Note: the # P is less than 0.01 and less than 0.05vs blank control group; * Model control group of P <0.05 vs
1.2 Effect experiment of herb paste of rhizoma Polygonati in embryo gallus Domesticus on Chronic hemolytic anemia
50 healthy C57BL/6 mice with the weight of 18-22g are pre-fed for 3d, and are randomly divided into a blank control group, a model control group and a high, medium and low dose groups of polygonatum cyrtonema herbal paste 2, 1 and 0.5g/kg according to the weight, wherein each group contains 10 mice. Except for the blank control group, each group was fed with 300mg/L phenylhydrazine solution, while each group of mice was gavaged with 20ml/kg of the corresponding drug solution, the blank and model control groups were fed with an equal volume of purified water once a day, continuously fed with 6wk, and fasted for 18 hours before the last administration. Mice were observed daily for signs of appearance from the start of dosing. After 1 hour of the last administration, blood was collected from the abdominal aorta after anesthesia, and blood indicators such as Red Blood Cells (RBC), hemoglobin (HGB), hematocrit (HCT), and red blood cell mean distribution width (RDW) were measured by a blood analyzer for each group of mice. The results are shown below:
1.2.1 Effect on Chronic hemolytic anemia model mouse morphology
The blank control group mice had normal activity; the fur has luster; the nose and lips are clean, moist and light pink; the waist and the back are straight, and the appetite is normal. The model control group showed excitation, dysphoria, and joy of getting a good stand in the first 4 weeks. With the prolonged feeding time, the anemia degree is increased, the ears, paws and other parts of the mice are obviously pale, and the mice have the behaviors of lassitude, reduced activity and tendency to curl and form masses. After the mice in the three dosage groups of the chicken embryo rhizoma polygonati herbal paste are administrated, compared with the model group, the mice are lightened in different degrees, the state is obviously better than that of the model group, and the improvement degree has obvious dose dependence.
1.2.2 Effect on the blood cell count of mice model of Chronic hemolytic anemia
Compared with a blank control group mouse, the RBC, HGB and HCT of the model control group mouse are obviously reduced, and the RDW is obviously increased, which indicates that the model building of the chronic hemolytic anemia model is successful. After 6wk of administration, RBC, HGB and HCT of the mice in the 2 g/kg and 1g/kg dose groups of the polygonatum sibiricum herbal paste are obviously increased, RDW is obviously reduced (P is less than 0.05 or P is less than 0.01), RBC, HGB and HCT in blood of the mice in the 0.5g/kg dose groups are also increased, and RDW is reduced, but the statistical significance is not achieved. The significant effect of the sealwort herb paste of chicken embryo in the dosage of more than 1g/kg on hemolytic anemia is shown in table 1.2.
TABLE 1.2 influence of herbal extract of Polygonatum Canaliculatum on the number of blood cells in hemolytic anemia model mice: (
n＝10)
Note: # P <0.01, and # P <0.05 vs blank control group; * P <0.05 > model control group
1.3 action of herbal paste of rhizoma Polygonati in chicken embryo on treating chemotherapy anemia
50 healthy SD rats weighing 180-220g are pre-fed for 7 days and are randomly divided into a blank control group, a model control group, a polygonatum sibiricum herbal paste 1.4, 0.7 and 0.35g/kg high, medium and low dose groups according to the weight, and each group comprises 10 rats. Except for the blank control group, the tail vein of each group was injected with 40mg/kg of carboplatin injection, while each group of rats was administered with 10ml/kg of the corresponding drug solution by gavage, the blank and model control groups were administered with an equal volume of purified water once a day, continuously administered at 4wk, and fasted for 18 hours before the last administration. Rats were observed daily for signs of appearance from the start of dosing. After 1 hour of the last administration, blood was collected from the abdominal aorta after anesthesia, and blood indicators such as Red Blood Cells (RBC), hemoglobin (HGB), platelets (PLT) and the like of each group of rats were measured by a hematology analyzer. The results are shown below:
1.3.1 Effect on morphology in rats model of chemoanemia
The rats in the blank control group have no obvious abnormality, and after the model control group rats are modeled, the animals in the model control group all show symptoms of reduced activity, fatigue, reduced activity, reduced ingestion and the like along with deepening of damage of modeling medicaments to animal organisms. After the rats in the three dose groups of the chicken embryo rhizoma polygonati herbal paste are administrated, compared with the model group, the rats are lightened to different degrees, the state is obviously better than that of the model control group, and the improvement degree has obvious dose dependence.
1.3.2 Effect on blood cell count in rats model of chemoanemia
Compared with a blank control group mouse, the model group mouse has the advantages that RBC, HGB and PLT are obviously reduced, and the model building success of the anemia model is shown. After 6wk of administration, compared with a model control group, RBC, HGB and PLT of mice in the 1.4 and 0.7g/kg dose groups of polygonatum sibiricum herbal paste are obviously increased (P is less than 0.05 or P is less than 0.01), and the four indexes in the blood of rats in the 0.35g/kg dose group are also increased, but the statistical significance is not provided. The significant effect of the herbal paste of rhizoma polygonati from chicken embryo on the chemotherapy anemia is shown in the table 1.3 when the dosage of the herbal paste is more than 0.7 g/kg.
TABLE 1.3 Effect of herbal paste of Polygonatum Canaliculatum on blood cell count of rats in model of chemoanemia: (
n＝10)
Note: # P <0.01 vs blank control group; * P <0.05 > P <0.01 vs model control group
2. Promoting reproduction
2.1 Effect of herb paste of Polygonatum sibiricum of chicken embryo on rat model with low reproductive function
After the female SD rat is subjected to adaptive feeding for 7d, the female SD rat is subjected to cytological examination of vaginal abscission, and the female SD rat with a normal estrus cycle is selected as a tested rat. Randomly selecting 10 blank control groups, administering 50mg/kg tripterygium glycosides tablet suspension (containing 0.05% sodium carboxymethylcellulose) to the rest rats every day, continuously intragastrically administering for 14d molding, and administering the same amount of physiological saline to the blank control groups. Rats after successful molding were randomly divided into 4 groups: model control group, chicken embryo rhizoma Polygonati herbal paste 1.4, 0.7, 0.35g/kg high, medium and low dosage groups. Each group of rats was gavaged with the corresponding drug solution 10ml/kg, and the blank and model control groups were given pure water of equal volume once a day for 4wk of continuous administration. Collecting blood from abdominal aorta after animal anesthesia after last administration, and measuring serum estradiol (E2), luteinizing Hormone (LH), and Follicle Stimulating Hormone (FSH) levels by radioimmunoassay; laparotomy, taking uterus and bilateral ovaries, weighing organ wet weight, and calculating organ coefficient. Organ coefficient = organ wet weight (mg)/body weight (g) × 100%. The results are as follows:
2.1.1 Effect of herbal paste of Polygonatum Canaliculatum on organ coefficient of rat with reproductive hypofunction model
Compared with the blank control group mouse, the uterus and ovary coefficients of the model control group rat are obviously reduced, which indicates that the model modeling of the reproductive hypofunction model is successful. After 4wk of administration, compared with a model control group, the uterine and ovarian coefficients of the rats in the 1.4 and 0.7g/kg dose groups of the polygonatum kingianum herbal paste are obviously increased (P is less than 0.05), and the uterine and ovarian coefficients of the rats in the 0.35g/kg dose group are also increased, but the statistical significance is not achieved. The herbal paste of rhizoma Polygonati in chicken embryo has obvious effect of improving and protecting uterus and ovary of rat with low reproductive function at dosage of 0.7g/kg or more, and is shown in Table 2.1.
TABLE 2.1 influence of herbal extract of Polygonatum cyrtonema Hua on organ coefficient of rat with reproductive hypofunction model: (
n＝10)
Note: # P <0.05 vs blank control group; * Model control group of P <0.05 vs
2.1.2 Effect of herbal paste of Polygonatum Canaliculatum on sex hormone level of rat model with low reproductive function
Compared with the blank control group rats, the serum E2 level of the model control group rats is obviously reduced, and the LH and FSH levels are obviously increased. After 4wk of administration, the E2 level, LH and FSH levels (P < 0.05) and hormone level of rats in 0.35g/kg dose group are increased and LH and FSH are reduced in the rats in 1.4 and 0.7g/kg dose groups of the polygonatum kingense herbal paste, compared with the model group, but the statistical significance is not achieved. The application shows that the polygonatum cyrtonema herbal paste has an obvious improvement effect on the reproductive function of a rat model with low reproductive function at a dosage of more than 0.7g/kg, and the result is shown in a table 2.2.
TABLE 2.2 Chicken embryo rhizoma Polygonati herbal paste to rat hormone level of reproductive hypofunction modelInfluence of (A)
n＝10)
Note: # P <0.05 # # P <0.01 vs blank control group; * Model control group of P <0.05 vs
2.2 action of herbal paste of rhizoma Polygonati in embryo gallus Domesticus on model mouse with premature ovarian failure
After the female Kunming mouse is adaptively raised for 3d, the vagina abscission cytology is checked, and the qualified mouse with normal estrus cycle is screened out. Randomly selecting 10 mice as blank control groups, injecting 30mg/kg cyclophosphamide into the rest mice by intraperitoneal injection every day for 1 time every day, continuously injecting for 20 days to replicate the premature ovarian failure model, and injecting the same amount of physiological saline into the mice of the blank control groups. Mice successfully molded were randomly divided into 4 groups: model control group, and high, medium and low dosage groups of 2, 1 and 0.5g/kg herbal paste of rhizoma Polygonati of embryo gallus Domesticus. Each group of rats was gavaged with the corresponding drug solution at 20ml/kg, and the blank and model control groups were given an equal volume of purified water once a day for 4wk of continuous administration. Blood is collected from abdominal aorta after anesthesia of the animals after the last administration, and the levels of estradiol (E2) and Follicle Stimulating Hormone (FSH) in serum are determined by adopting an Elisa method; laparotomy, taking uterus and bilateral ovaries, weighing organ wet weight, calculating organ coefficient, wherein the organ coefficient = organ wet weight (mg)/body weight (g) × 100%, and detecting the apoptosis condition of ovarian granule cells by referring to TUNEL apoptosis detection kit instructions. The results were as follows:
2.2.1 Effect of Polygonatum sibiricum extract on organ coefficients of premature ovarian failure model mice
Compared with the blank group of mice, the uterus and ovary coefficients of the model group of mice are obviously reduced, which indicates that the premature ovarian failure model is successfully modeled. After 4wk of administration, compared with the model group, the uterine and ovarian coefficients of the chicken embryo rhizoma polygonati herbal paste 2 and the mouse with the dosage of 1g/kg are obviously increased (P is less than 0.05), and the uterine and ovarian coefficients of the mouse with the dosage of 0.5g/kg are also increased, but the statistical significance is not achieved. The herbal ointment of rhizoma polygonati from chicken fetus has obvious improvement and protection effects on the uterus and ovary of premature ovarian failure model mice at the dosage of more than 1g/kg, and is shown in table 2.3.
TABLE 2.3 Effect of herbal paste of Polygonatum Canadensis Hemsl on organ coefficients of model mouse with premature ovarian failure: (
n＝10)
Note: (iii) blank control group # P <0.05 vs; * Model control group of P <0.05 vs
2.2.2 Effect of herbal extract of rhizoma Polygonati in Chicken embryo on sexual hormone level of model mouse with premature ovarian failure
Compared with the blank control group mice, the serum E2 level of the model control group mice is obviously reduced, and the FSH level is obviously increased. After 4wk of administration, the level of E2 in the polygonatum sibiricum herbal paste 2 and mice in the 1g/kg dose group was significantly increased, the level of FSH was decreased (P < 0.05), and the level of E2 in the mice in the 0.5g/kg dose group was also increased, and FSH was decreased, but there was no statistical significance. The application shows that the polygonatum cyrtonema herbal paste has obvious improvement effect on the ovary function of the premature ovarian failure model mouse at the dosage of more than 1g/kg, and the table is 2.4.
TABLE 2.4 influence of herbal extract of Polygonatum Canaliculatum Hemsl on hormone levels in model mice with premature ovarian failure (II)
n＝10)
Note: # P <0.05 # # P <0.01 vs blank control group; * Model control group of P <0.05 vs
2.2.3 Effect of herbal paste containing rhizoma Polygonati in Chicken embryo on apoptosis of ovary granular cells of model mouse with premature ovarian failure
The blank granular cells have lighter apoptosis degree and fewer apoptosis positive cells. Compared with the blank group, the apoptosis positive cells of the model group mice are obviously increased, and the apoptosis average optical density value is obviously increased (P < 0.05). After being treated by the polygonatum sibiricum herbal paste, compared with a model group, the polygonatum sibiricum herbal paste obviously reduces the number of apoptosis granular cells of mice in a high-dosage and medium-dosage group, and obviously reduces the apoptosis average optical density value (P is less than 0.05). The herbal ointment of the polygonatum cyrtonema Hance can effectively inhibit the apoptosis of ovarian granular cells of a chemotherapy-induced premature ovarian failure model mouse, delay the premature ovarian aging process and improve and recover the ovarian function, and is shown in table 2.5.
TABLE 2.5 Effect of herbal extract of Polygonati officinalis rhizoma in Chicken embryo on apoptosis of ovary granulosa cells in model mouse with premature ovarian failure ((
n＝10)
Note: # P <0.01 vs blank control group; * Model control group of P <0.05 vs
The present invention is not limited to the real-time mode, and any structural changes made under the teaching of the present invention shall fall within the protection scope of the present invention, all the technical solutions that are the same as or similar to the present invention.
The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.
Claims (8)
1. The chicken embryo yolk essence herbal paste is characterized in that: comprises the following components: 50-70 parts of chicken embryo, 70-80 parts of rhizoma polygonati, 40-60 parts of wolfberry fruit, 90-110 parts of mulberry, 40-60 parts of raspberry, 70-80 parts of Chinese yam, 140-160 parts of poria cocos, 20-40 parts of liquorice, 1-10 parts of donkey-hide gelatin, 70-80 parts of longan pulp, 140-160 parts of Chinese date, 40-60 parts of dried ginger, 50-70 parts of peach kernel and 40-60 parts of double-petal red rose.
2. A production method of chicken embryo yolk essence herbal paste is characterized by comprising the following steps: the method comprises the following steps:
step 1, smashing donkey-hide gelatin, and melting to obtain donkey-hide gelatin solution for later use;
step 2, dynamically extracting chicken embryo, rhizoma polygonati, medlar, mulberry, raspberry, chinese yam, tuckahoe, liquorice, longan aril, chinese date, dried ginger, peach kernel and double-petal red rose by using water vapor to obtain an extracting solution, and concentrating under reduced pressure to obtain a concentrated solution;
and 3, adding activated carbon, heating, filtering, adding the dissolved donkey-hide gelatin solution into the filtrate, concentrating under normal pressure, filling, and sterilizing to obtain a finished product.
3. The method for producing herbal extract of chicken fetal jaundice as claimed in claim 2, wherein the method comprises the following steps: the method for melting the melting of the donkey-hide gelatin in the step 1 comprises the following steps: taking a proper amount of donkey-hide gelatin, crushing, taking water with the amount of 3-8 times of that of the donkey-hide gelatin, boiling, adding donkey-hide gelatin powder, and stirring to dissolve to obtain a donkey-hide gelatin solution.
4. The method for producing chicken fetal jaundice herbal paste as claimed in claim 2, wherein the method comprises the following steps: the method for dynamically extracting the water vapor in the step 2 comprises the following steps: putting the rest medicinal materials in a multifunctional extraction tank, adding 6-10 times of drinking water, decocting for 2 hr, filtering, adding 5-8 times of drinking water, decocting for 2 hr, and filtering; the decocting steam pressure is 0.1-0.3Mpa, and the time for decocting starts to be timed when the liquid medicine is boiling.
5. The method for producing chicken fetal jaundice herbal paste as claimed in claim 2, wherein the method comprises the following steps: and 2, carrying out reduced pressure concentration in the step 2 by adopting a double-effect concentration method, merging decoction, putting the decoction in the same double-effect concentrator, and concentrating the decoction until the relative density is 1.10-1.20 and the testing temperature is 70-90 ℃ to obtain concentrated solution.
6. The method for producing herbal extract of chicken fetal jaundice as claimed in claim 5, wherein the method comprises the following steps: the control parameters in the concentration process of the double-effect concentrated water are as follows:
steam pressure: <0.25Mpa;
vacuum degree: the first effect vacuum is controlled to be-0.02 to-0.04 Mpa, and the second effect vacuum is controlled to be-0.04 to-0.06 Mpa;
evaporation temperature: the first effect temperature is controlled to be 50-95 ℃, and the second effect temperature is controlled to be 45-85 ℃.
7. The method for producing chicken fetal jaundice herbal paste as claimed in claim 2, wherein the method comprises the following steps: and 3, adding 2-5% of activated medicinal activated carbon into the concentrated solution, fully stirring, sucking into a settling tank, boiling for 10-20 minutes, cooling to 45-50 ℃, and filtering to be clear.
8. The method for producing herbal extract of chicken fetal jaundice as claimed in claim 2, wherein the method comprises the following steps: and (3) when the temperature of the concentrated solution filtered in the step (3) is 45-50 ℃, adding the donkey-hide gelatin solution, and continuously concentrating to the required relative density, wherein the range of the relative density is 1.20-1.40.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238843A (en) * | 2013-05-24 | 2013-08-14 | 江苏海昇药业有限公司 | Skin moisturizing paste and preparation method thereof |
| CN109620883A (en) * | 2018-12-12 | 2019-04-16 | 怀化正好制药有限公司 | Donkey-hide gelatin lengthens one's life oral solution and preparation method thereof |
| CN110075202A (en) * | 2019-05-22 | 2019-08-02 | 湖南众一医药咨询有限公司 | A kind of qi-blood tonifying Chinese medicine composition and preparation method thereof |
| CN112107612A (en) * | 2020-10-16 | 2020-12-22 | 山东宏济堂制药集团股份有限公司 | Traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as well as preparation method and application thereof |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238843A (en) * | 2013-05-24 | 2013-08-14 | 江苏海昇药业有限公司 | Skin moisturizing paste and preparation method thereof |
| CN109620883A (en) * | 2018-12-12 | 2019-04-16 | 怀化正好制药有限公司 | Donkey-hide gelatin lengthens one's life oral solution and preparation method thereof |
| CN110075202A (en) * | 2019-05-22 | 2019-08-02 | 湖南众一医药咨询有限公司 | A kind of qi-blood tonifying Chinese medicine composition and preparation method thereof |
| CN112107612A (en) * | 2020-10-16 | 2020-12-22 | 山东宏济堂制药集团股份有限公司 | Traditional Chinese medicine composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 美敦宜: "女人的心思，不得不猜", pages 1 - 8, Retrieved from the Internet <URL:https://mp.weixin.qq.com/> * |
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