CN112075430A - Trifloxystrobin-containing bactericidal composition - Google Patents
Trifloxystrobin-containing bactericidal composition Download PDFInfo
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- CN112075430A CN112075430A CN201910518566.6A CN201910518566A CN112075430A CN 112075430 A CN112075430 A CN 112075430A CN 201910518566 A CN201910518566 A CN 201910518566A CN 112075430 A CN112075430 A CN 112075430A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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Abstract
The invention discloses a sterilization composition containing trifloxystrobin and an application thereof, wherein effective components of the sterilization composition contain trifloxystrobin and metrafenone, and the weight ratio of the trifloxystrobin to the metrafenone is 1: 80-80: 1, preferably 1: 50-50: 1. The sum of the weight of the metrafenone and the metrafenone accounts for 1-90 percent of the composition, and preferably 10-60 percent. The present compositions may be formulated into agriculturally acceptable dosage forms. The invention has reasonable components and good bactericidal effect, and the activity and the bactericidal effect of the invention are not the simple superposition of the components, compared with a single agent, the invention has obvious bactericidal effect and good safety to crops. The invention has obvious effect of preventing and treating powdery mildew.
Description
Technical Field
The invention relates to a sterilization composition, in particular to a sterilization composition containing trifloxystrobin, belonging to the technical field of pesticide compounding.
Background
Trifloxystrobin (Trifloxystrobin, CAS number: 141517-21-7, chemical name: (E) -methoxyimino) - { (E) -alpha- [1- (alpha, alpha-trifluoro-tolyl) ethyleneaminooxy ] o-tolyl } acetic acid methyl ester) is a methoxy acrylate bactericide, is a quinone outside inhibitor, and inhibits mitochondrial respiration by preventing electron transfer in the center of cytochrome bc1 Qo; is a semi-systemic and broad-spectrum bactericide and has a protective effect; the concrete characteristics are as follows: realizing redistribution of the medicament on the plant through the movement of surface steam and surface water; is mainly used for preventing and treating diseases of ascomycetes, deuteromycetes, basidiomycetes and oomycetes.
Metrafenone (Ectafenone, CAS number: 220899-03-6, chemical name: 3-bromo-6 '-methoxy-2' -methylphenyl) - (2,3, 4-trimethoxy-6-methylphenyl) methanone) is a benzophenone bactericide and has preventive, therapeutic and anti-spore activities; has inhibitory effect on growth of both germ tube and hypha; can interfere with the establishment and maintenance of polar actin organization.
At present, no literature report on the prevention and treatment of powdery mildew by mixing trifloxystrobin and metrafenone exists, and further experiments show that the mixing of trifloxystrobin and metrafenone with different action mechanisms has an obvious synergistic effect, can obviously improve the prevention and treatment effect on the powdery mildew, reduce the dosage, reduce the prevention and treatment cost and have high safety on crops.
Disclosure of Invention
The invention aims to provide a composition and a bactericidal composition prepared from the composition, which has the advantages of good bactericidal effect, low medication cost and safety to crops.
The invention also aims to provide a preparation formulation of the bactericidal composition containing trifloxystrobin and metrafenone.
Still another object of the present invention is to provide the use of the above composition for controlling powdery mildew of crops.
The technical scheme of the invention is as follows:
the trifloxystrobin-containing bactericidal composition comprises trifloxystrobin and metrafenone;
the bactericidal composition comprises trifloxystrobin and metrafenone in a weight ratio of 1: 80-80: 1;
further, the bactericidal composition has the weight ratio of trifloxystrobin to metrafenone of 1: 50-50: 1;
further, the bactericidal composition has the weight ratio of trifloxystrobin to metrafenone of 1: 20-20: 1;
further, the bactericidal composition has the weight ratio of trifloxystrobin to metrafenone being 1: 10-10: 1;
further, the bactericidal composition has the weight ratio of trifloxystrobin to metrafenone being 1: 3-3: 1;
the bactericidal composition comprises 1-90% of the composition, preferably 10-60% of the composition, by weight of the active ingredients trifloxystrobin and metrafenone.
The bactericidal composition can be prepared into agriculturally acceptable preparations;
further, the composition comprises an active ingredient and an auxiliary ingredient of a pesticide preparation;
further, the auxiliary components of the pesticide preparation comprise a carrier and an auxiliary agent;
further, the carrier is any one, two or three of water, a solvent or a filler, and the water is preferably deionized water;
further, the solvent is selected from one or a mixture of more of N, N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile;
further, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;
furthermore, the auxiliary agent at least comprises a surfactant, and other functional auxiliary agents such as an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent, a defoaming agent and the like can be added according to different use occasions and requirements;
further, the surfactant is selected from one or more of an emulsifier, a dispersant, a wetting agent or a penetrant;
further, the other functional auxiliary agents are selected from one or more of an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent or an antifoaming agent;
further, the emulsifier is selected from one or more of agricultural milk 500# (calcium alkyl benzene sulfonate), OP series phosphate ester (nonylphenol polyoxyethylene ether phosphate ester), 600# phosphate ester (phenylphenol polyoxyethylene ether phosphate ester), styrene polyoxyethylene ether ammonium sulfate salt, magnesium salt of alkyl diphenyl ether disulfonate, triethanolamine salt, agricultural milk 400# (benzyldimethylphenol polyoxyethylene ether), agricultural milk 700# (alkylphenol formaldehyde resin polyoxyethylene ether), Ningru 36# (phenethylphenol formaldehyde resin polyoxyethylene ether), agricultural milk 1600# (phenethylphenol polyoxyethylene polyoxypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), agricultural dehydrated milk 33# (alkylaryl polyoxyethylene polyoxypropylene ether), span series (sorbitan monostearate), Tween series (sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether) A mixture of seed constituents;
further, the dispersing agent is selected from one or a mixture of a plurality of polycarboxylate, lignosulfonate, alkylphenol polyoxyethylene formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, sodium salt of naphthalene sulfonic acid formaldehyde polymer, alkylphenol polyoxyethylene, fatty amine polyoxyethylene, fatty acid polyoxyethylene or glycerol fatty acid ester polyoxyethylene;
further, the wetting agent is selected from one or a mixture of more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrant F, Chinese honeylocust fruit powder, silkworm excrement or soapberry powder;
further, the penetrating agent is selected from a mixture consisting of one or more of penetrating agent JFC (fatty alcohol-polyoxyethylene ether), penetrating agent T (diisooctyl maleate sulfonate), azone or organic silicon;
further, the antifreezing agent is a mixture consisting of one or more of ethylene glycol, propylene glycol, glycerol or urea;
further, the thickening agent is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;
further, the stabilizer is selected from one or more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate;
further, the disintegrating agent is selected from one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;
further, the defoaming agent is selected from one or a mixture of more of silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds or C8-C10 fatty alcohol compounds;
all of the above are commercially available.
The compositions of the present invention may be formulated into pesticidally acceptable dosage forms, including solid, liquid, seed treatment, other formulations, according to methods well known to those skilled in the art;
further, the solid preparation is powder, dispersible tablets, granules, soluble powder, soluble granules, soluble tablets, milk powder, milk granules, water dispersible granules, wettable powder, micro-capsule granules, powder, macro-granules and water dispersible tablets;
further, the liquid preparation is microcapsule suspending agent, dispersible agent, missible oil, emulsion granules, oil emulsion, emulsion powder, aqueous emulsion, microemulsion, dispersible oil suspending agent, oil dispersion powder, suspending agent, suspoemulsion, soluble solution and ultra-low volume liquid;
further, the seed treatment preparation is a suspended seed coating agent, a seed treatment dry powder agent, a seed treatment dispersible powder agent, a seed treatment suspending agent and a seed treatment liquid agent;
further, the other preparations are bait, concentrated bait, gas preparation, aerosol, smoke agent;
furthermore, the preparation formulation is preferably wettable powder, water dispersible granules, suspending agents, microemulsion, missible oil and emulsion in water;
further, the composition is prepared into wettable powder, and the components and the content of the wettable powder are preferably as follows: 0.1 to 80 percent of trifloxystrobin, 0.1 to 80 percent of metrafenone, 2 to 10 percent of dispersant, 2 to 10 percent of wetting agent and the balance of filler;
further, the composition is prepared into water dispersible granules, and the components and the content of the water dispersible granules are preferably as follows: 0.1-80% of trifloxystrobin, 0.1-80% of metrafenone, 3-12% of a dispersant, 1-8% of a wetting agent, 1-10% of a disintegrating agent and the balance of a filler;
further, the composition is prepared into a suspending agent, and the components and the content of the suspending agent are preferably as follows: 0.1-80% of trifloxystrobin, 0.1-80% of metrafenone, 2-10% of a dispersant, 2-10% of a wetting agent, 0.01-2% of a defoaming agent, 0-2% of a thickening agent, 0-8% of an antifreeze agent and the balance of deionized water;
further, the composition is prepared into a microemulsion, and the components and the content of the microemulsion are preferably as follows: 0.1 to 80 percent of trifloxystrobin, 0.1 to 80 percent of metrafenone, 10 to 25 percent of solvent, 5 to 15 percent of emulsifier and the balance of deionized water;
further, the composition is prepared into missible oil, and the missible oil preferably comprises the following components in percentage by weight: 0.1 to 80 percent of trifloxystrobin, 0.1 to 80 percent of metrafenone, 5 to 15 percent of emulsifier and the balance of solvent;
further, the composition is prepared into an aqueous emulsion, and the components and the content of the composition are preferably as follows: 0.1 to 80 percent of trifloxystrobin, 0.1 to 80 percent of metrafenone, 0 to 5 percent of disintegrating agent, 5 to 15 percent of emulsifying agent and the balance of deionized water;
the invention also discloses the application of the bactericidal composition to the control of crop diseases;
further, the disease is powdery mildew;
further, the crops are cucumbers and/or strawberries;
furthermore, the crop diseases are cucumber powdery mildew and/or strawberry powdery mildew.
The invention also discloses a method for controlling crop diseases, which is to apply the bactericidal composition to the growing area and/or leaves and/or stems of crops;
further, the disease is powdery mildew;
further, the crops are cucumbers and/or strawberries;
furthermore, the crop diseases are cucumber powdery mildew and/or strawberry powdery mildew.
Compared with the prior art, the invention has the following beneficial effects: the bactericidal composition disclosed by the invention can generate a very high synergistic effect, can obviously improve the control effect on powdery mildew, reduce the dosage, reduce the control cost and has high safety on crops.
Detailed Description
The present invention will be described in more detail with reference to examples. The practice of the invention is not limited to the following examples, and any variations or modifications in practice of the invention are intended to fall within the scope of the invention. The methods in the following examples are conventional in the art unless otherwise specified.
Preparation of the preparation
Example 1: 10% trifloxystrobin-metrafenone wettable powder (5:5)
The formula is as follows: 5% of trifloxystrobin, 5% of metrafenone, 3% of calcium lignosulfonate, 5% of sodium alkyl naphthalene formaldehyde condensate, 2% of sodium alkyl sulfonate and argil, and the balance is made up;
the preparation method comprises the following steps: the wettable powder preparation of the composition can be prepared by mixing the effective components of trifloxystrobin and metrafenone with a dispersant, a wetting agent and a filler according to the formula, uniformly stirring in a stirring kettle, crushing by a jet mill, and uniformly mixing.
Example 2: 15% trifloxystrobin-metrafenone wettable powder (10:5)
The formula is as follows: 10% of trifloxystrobin, 5% of metrafenone, 4% of calcium alkylphenol polyoxyethylene ether sulfonate, 4% of alkyl naphthalene sulfonate formaldehyde condensate, 2% of alkyl naphthalene sodium sulfonate and talcum powder, and the balance is made up;
the preparation method comprises the following steps: the same as in example 1.
Example 3: 25% trifloxystrobin-metrafenone wettable powder (10:15)
The formula is as follows: 10% of trifloxystrobin, 15% of metrafenone, 5% of sodium alkyl naphthalene formaldehyde condensate, 3% of sodium polycarboxylate, 3% of sodium dodecyl sulfate and kaolin, and the balance of the mixture;
the preparation method comprises the following steps: the same as in example 1.
Example 4: 10% trifloxystrobin-metrafenone water dispersible granule (4:6)
The formula is as follows: 4% of trifloxystrobin, 6% of metrafenone, 3% of polycarboxylate GY-D06, 3% of alkyl naphthalene sulfonate formaldehyde condensate, 3% of sodium dodecyl benzene sulfonate, 5% of sodium naphthalene sulfonate, 2% of sodium carboxymethylcellulose and the balance of attapulgite;
the preparation method comprises the following steps: the active ingredients of trifloxystrobin, metrafenone, a dispersing agent, a wetting agent, a disintegrating agent and a filler are uniformly mixed, crushed by an ultramicro jet mill, kneaded, added into a fluidized bed granulation dryer for granulation, drying and screening, and then subjected to sampling analysis, so that the water dispersible granule preparation of the composition can be prepared.
Example 5: 30% trifloxystrobin-metrafenone water dispersible granule (15:15)
The formula is as follows: 15% of trifloxystrobin, 15% of metrafenone, 2% of sodium alkyl naphthalene sulfonate, 4% of sodium lignin sulfonate, 2% of sodium dibutyl naphthalene sulfonate, 3% of sodium dodecyl sulfate, 1% of urea, 2% of polyvinyl alcohol and argil for balancing;
the preparation method comprises the following steps: the same as in example 4.
Example 6: 15% trifloxystrobin-metrafenone water dispersible granule (10:5)
The formula is as follows: 10% of trifloxystrobin, 5% of metrafenone, 5% of a dispersing agent NNO, 3% of sodium lignosulfonate, 4% of sodium dibutylnaphthalene sulfonate, 3% of sodium dodecyl benzene sulfonate, 1.5% of sodium bicarbonate, 2% of starch and talcum powder, wherein the balance is added;
the preparation method comprises the following steps: the same as in example 4.
Example 7: 20% trifloxystrobin-metrafenone suspending agent (10:10)
The formula is as follows: 10% of trifloxystrobin, 10% of metrafenone, 3% of sodium lignosulfonate, 3% of phenethyl phenol polyoxyethylene ether phosphate, 4% of sodium dodecyl sulfate, 0.5% of carboxymethyl cellulose, 3% of polyethylene glycol, 0.2% of isothiazolinone, 1% of octanol and deionized water, and the balance is made up;
the preparation method comprises the following steps: mixing the auxiliary agents, uniformly mixing the auxiliary agents through high-speed shearing, adding the effective components of trifloxystrobin and metrafenone, and grinding balls in a ball grinding machine for 2-3 hours to enable the particle diameters to be below 5mm, so that the suspending agent preparation of the composition can be prepared.
Example 8: 30% trifloxystrobin-metrafenone suspending agent (10:20)
The formula is as follows: 10% of trifloxystrobin, 20% of metrafenone, 3% of sodium lignin sulfonate, 3% of sodium naphthalene sulfonate, 4% of sodium dodecyl sulfonate, 0.5% of carboxymethyl cellulose, 3% of magnesium aluminum silicate, 0.2% of propylene glycol, 1% of hexanol and deionized water to make up the balance;
the preparation method comprises the following steps: the same as in example 7.
Example 9: 40% trifloxystrobin-metrafenone suspending agent (10:30)
The formula is as follows: 10% of trifloxystrobin, 30% of metrafenone, 3% of phenethyl phenol polyoxyethylene ether phosphate, 4% of sodium dodecyl sulfate, 0.5% of carboxymethyl cellulose, 3% of magnesium aluminum silicate, 1% of octanol and deionized water, wherein the balance is made up;
the preparation method comprises the following steps: the same as in example 7.
Example 10: 20% trifloxystrobin-metrafenone microemulsion (10:10)
The formula is as follows: 10% of trifloxystrobin, 10% of metrafenone, 3% of Nongru 1601, 3% of calcium dodecylbenzene sulfonate, 3% of methanol, 3% of xylene, 1% of epichlorohydrin and deionized water;
the preparation method comprises the following steps: the effective components of trifloxystrobin and metrafenone are completely dissolved by a solvent and a cosolvent, then other auxiliary agents are added and uniformly mixed, finally water is added, and the microemulsion preparation of the composition can be prepared after full stirring.
Example 11: 40% trifloxystrobin-metrafenone microemulsion (10:30)
The formula is as follows: 10% of trifloxystrobin, 30% of metrafenone, 5% of Nongru 600#, 3% of isopropanol, 3% of toluene, 1% of triphenyl phosphite and deionized water for supplementing the balance;
the preparation method comprises the following steps: the same as in example 10.
Example 12: 10% trifloxystrobin-metrafenone microemulsion (10:5)
The formula is as follows: 10% of trifloxystrobin, 5% of metrafenone, 3% of Nongru 700#, 3% of Nongru 2201, 3% of n-butanol, 2% of toluene, 1% of epichlorohydrin and deionized water for the balance;
the preparation method comprises the following steps: the same as in example 10.
Example 13: 20% trifloxystrobin-metrafenone missible oil (10:10)
The formula is as follows: 10% of trifloxystrobin, 10% of metrafenone, 3% of Nongru No. 600, 5% of span-60, 2% of methanol, 2% of epichlorohydrin and xylene for making up the balance;
the preparation method comprises the following steps: adding the active ingredients of trifloxystrobin and metrafenone into a carrier, adding a surfactant and other functional auxiliaries, and uniformly stirring and mixing in a stirring and mixing kettle to prepare the missible oil preparation of the composition.
Example 14: 30% trifloxystrobin-metrafenone missible oil (10:20)
The formula is as follows: 10% of trifloxystrobin, 20% of metrafenone, 5% of an emulsifier T-60, 5% of Nongru 1601#, 2% of cyclohexanone, 2% of acetic anhydride and toluene to make up the balance;
the preparation method comprises the following steps: the same as in example 13.
Example 15: 50% trifloxystrobin-metrafenone missible oil (25:25)
The formula is as follows: 25% of trifloxystrobin, 25% of metrafenone, 5% of an emulsifier T-60, 5% of Nongru 1601#, 2% of acetone, 2% of triphenyl phosphite and biodiesel, and the balance is made up;
the preparation method comprises the following steps: the same as in example 13.
Example 16: 30% trifloxystrobin-metrafenone aqueous emulsion (10:20)
The formula is as follows: 10% of trifloxystrobin, 20% of metrafenone, 5% of Nongru 1601, 2% of toluene, 1% of triphenyl phosphite, 2% of propylene glycol, 5% of bentonite, 0.2% of sodium benzoate and deionized water to make up the balance;
the preparation method comprises the following steps: the active ingredients of trifloxystrobin, metrafenone and an auxiliary agent are added together to be dissolved into a uniform oil phase, the oil phase is added into a water phase while stirring at a high speed in a reaction kettle, water is slowly added until a phase inversion point is reached, a shearing machine is started to carry out high-speed shearing, and the rest water is added and sheared for about half an hour, so that the aqueous emulsion preparation of the composition can be prepared.
Example 17: 20% trifloxystrobin-metrafenone aqueous emulsion (10:10)
The formula is as follows: 10% of trifloxystrobin, 10% of metrafenone, 6% of tween-60 #, 2% of cyclohexanone, 1% of epoxidized soybean oil, 2% of ethylene glycol, 5% of polyvinyl alcohol, 0.2% of methanol and deionized water to make up the balance;
the preparation method comprises the following steps: the same as in example 16.
Example 18: 40% trifloxystrobin-metrafenone aqueous emulsion (30:10)
The formula is as follows: 30% of trifloxystrobin, 10% of metrafenone, 2% of span-60 #, 4% of Nongru 400#, 2% of dimethylbenzene, 2% of sodium chloride, 0.2% of benzoic acid and deionized water for balancing;
the preparation method comprises the following steps: the same as in example 16.
Active case
The type of the combined action of the mixing of trifloxystrobin and metrafenone on the powdery mildew of crops is illustrated by the combined action measurement of the mixing.
Example 19: and (3) measuring the combined action of the mixture of trifloxystrobin and metrafenone on cucumber powdery mildew.
The test basis is as follows: NY/T1156.11-2008 "indoor bioassay of pesticides test criteria fungicides part 11: experimental potting method for preventing and treating powdery mildew of melons
Test targets: cucumber powdery mildew (Erysiphe cichororaceae), and the cucumber is cultivated in a pot to 2-4 true leaves and numbered for later use.
Test agents: the 98 percent trifloxystrobin active compound and the 98 percent metrafenone active compound are provided by research and development centers of Hilier pharmaceutical industry group Limited.
Spore suspension preparation: washing fresh spores on plant leaves full of powdery mildew with purified water containing small amount of Tween-80, filtering with double-layer gauze to obtain spore with concentration of 1 × 105spores/mL of suspension, ready for use.
Preparing a medicament: respectively weighing 0.1053g of trifloxystrobin active compound and metrafenone active compound, respectively dissolving in 2.00g of N-dimethylformamide, respectively diluting with 0.1% tween-80 aqueous solution, diluting to a constant volume of 100mL, stirring uniformly, and preparing into 1000mg/L mother liquor;
blank control mother liquor: weighing 2.00g of N-dimethylformamide, diluting with 0.1% Tween-80 aqueous solution and fixing the volume to 100 mL. The prepared liquid medicine is set with 5 treatment concentration gradients for standby.
Medicament treatment: the liquid medicine is uniformly sprayed on the leaves until the leaves are completely wetted, and the liquid medicine is naturally dried for later use. Each treatment was performed in 3 pots, 4 replicates, and a blank was set.
Inoculation and culture: the spore suspension was spray inoculated. The inoculated test material is naturally air-dried, then moved to a greenhouse and cultured for 7 days at the temperature of 20-24 ℃.
And (3) investigation: when the blank control diseased leaf rate reaches more than 80%, investigating the diseased situation of each treatment in a grading way, investigating at least 30 leaves in each treatment, wherein the grading standard is as follows:
no disease spot at level 0;
grade 1, the lesion area accounts for less than 5% of the whole leaf area;
grade 3, the lesion area accounts for 6 to 10 percent of the whole leaf area;
grade 5, the lesion area accounts for 11 to 20 percent of the whole leaf area;
7 grade, the lesion area accounts for 21 to 40 percent of the whole leaf area;
grade 9, the lesion area accounts for more than 40% of the whole leaf area.
Data statistics and analysis:
calculating disease index, and calculating the relative prevention effect of each concentration according to the disease index.
Disease index [ ∑ (number of diseased leaves at each stage × relative stage)/(total number of investigated leaves × 9) ] × 100
Control effect (%) [ (control disease index-treatment disease index)/control disease index ] × 100
Calculation of EC for each agent Using DPS software50The values and corresponding b values, correlation coefficients.
SR＝EC50(theoretical value)/EC50(observed value)
EC50EC of (theoretical value) ═ a + b)/(a/a50EC of + B/B50)
Wherein: a is trifloxystrobin and B is metrafenone; a is the percentage content of trifloxystrobin and b is the percentage content of metrafenone in the combination.
The combined Synergistic Ratio (SR) of the two medicaments in different proportions is calculated according to the Wadley method, the antagonism is achieved when the SR is less than 0.5, the addition effect is achieved when the SR is more than or equal to 0.5 and less than or equal to 1.5, and the synergism is achieved when the SR is more than 1.5.
Test results and analysis:
trifloxystrobin, metrafenone and the indoor toxicity test results of cucumber powdery mildew mixed and matched with the two in different proportions (see table 1), and the toxicity regression equation, the correlation coefficient r and the EC of each medicament50Value, theoretical EC50The values and the Synergy Ratio (SR) are shown in Table 1.
TABLE 1 Combined effect of trifloxystrobin and metrafenone on cucumber powdery mildew
As can be seen from Table 1, the EC of trifloxystrobin and metrafenone on powdery mildew of cucumber50The values were 1.5234mg/l and 1.6153mg/l, respectively. The trifloxystrobin and metrafenone are mixed, the corresponding synergistic ratios are 1.2889, 1.6012, 2.0686, 1.7708 and 1.5056 when the ratios are 3:1, 2:1, 1:2 and 1:3 respectively, and the synergistic effect on cucumber powdery mildew is shown to a certain degree when the ratios are 2:1, 1:2 and 1: 3. Wherein, the SR value of the ratio of 1:1 is relatively large, and further verification is needed by field experiments on how the field efficacy is.
TABLE 2 original data of combined toxicity test results of trifloxystrobin and metrafenone mixed for powdery mildew of cucumber
Test of field drug effect
Example 20: and (3) field test for preventing and controlling cucumber powdery mildew.
Test site: the test bases are a test base of Shouguanshi of Shandong province and a test base of Mitsui of Hainan province.
Test agents: 20% trifloxystrobin-metrafenone emulsifiable concentrate, 20% trifloxystrobin-metrafenone suspending agent, 20% trifloxystrobin-metrafenone microemulsion, 30% trifloxystrobin-metrafenone suspending agent, 30% trifloxystrobin-metrafenone emulsifiable concentrate, 25% trifloxystrobin suspending agent, 42% metrafenone suspending agent and clear water control.
Test targets: cucumber powdery mildew (Erysiphe cichororaceae).
The application time and times are as follows: the medicine is applied once every 6 months and 22 days in 2018; the first application is carried out 3 months and 26 days in 2019, and the second application is carried out 7 days later.
The test agents and dosages are detailed in tables 3 and 4. A blank control was also set, with 4 replicates per treatment, 20m per cell2The cells are randomly arranged. The spraying agent is uniformly sprayed on the front and back surfaces of the blade by a conventional spraying method.
The investigation and statistical method comprises the following steps: the disease condition base was investigated before application and 7 days after the last application. Four points were randomly selected per cell, and 1-5 total leaves were investigated per point, each leaf being graded as the percentage of lesion area.
The grading method comprises the following steps:
level 0: no disease spots;
level 1: the lesion area accounts for less than 5% of the whole leaf area;
and 3, level: the lesion area accounts for 6 to 10 percent of the whole leaf area;
and 5, stage: the lesion area accounts for 11 to 20 percent of the whole leaf area;
and 7, stage: the lesion area accounts for 21 to 40 percent of the whole leaf area;
and 9, stage: the lesion area accounts for more than 40% of the whole leaf area.
Disease index [ Σ (each stage of diseased leaf number × relative stage number)/(total investigated leaf number × 9) ] × 100
The prevention and treatment effect (%) is [1- (disease index before control drug application x disease index after treatment drug application)/(disease index after control drug application x disease index before treatment drug application ] × 100%
TABLE 3 test results of mixing trifloxystrobin and metrafenone for controlling cucumber powdery mildew (Shouguang)
Note: test site: shandong province Shouguang City, application time at 1 st time: in 2018, day 22, 6 months, 1 strain was investigated at each point.
TABLE 4 results of the experiment on the control of powdery mildew of cucumber by mixing trifloxystrobin and metrafenone (III)
Note: test site: san city, Hainan province, time to first application: the second application was carried out 3/26 in 2019 at 7-day intervals, and 10 plants were investigated each time.
The field efficacy results show that (see table 3 and table 4), the control effect of 20% trifloxystrobin-metrafenone missible oil, 20% trifloxystrobin-metrafenone suspending agent, 20% trifloxystrobin-metrafenone microemulsion, 30% trifloxystrobin-metrafenone suspending agent and 30% trifloxystrobin-metrafenone missible oil on powdery mildew of cucumber is more than 80%, and is obviously higher than that of a single agent.
The safety of the cucumbers is investigated, and after the cucumber is applied, the cucumber is not harmed by each treatment.
TABLE 5 original data of experiment for controlling cucumber powdery mildew by mixing trifloxystrobin and metrafenone (Shouguang)
TABLE 6 original data of experiment for controlling powdery mildew of cucumber by mixing trifloxystrobin and metrafenone (III)
Example 21: test for preventing and treating powdery mildew of strawberry by mixing trifloxystrobin and metrafenone
Test site: shandong Qingdao is the test base of the wind shift store in Mo city.
Test agents: 20% trifloxystrobin-metrafenone emulsifiable concentrate, 20% trifloxystrobin-metrafenone suspending agent, 20% trifloxystrobin-metrafenone microemulsion, 30% trifloxystrobin-metrafenone suspending agent, 30% trifloxystrobin-metrafenone emulsifiable concentrate, 25% trifloxystrobin suspending agent, 42% metrafenone suspending agent and clear water control.
Test targets: strawberry powdery mildew (Sphaerotheca macularis).
The application time and times are as follows: the first application is carried out 7 months and 14 days in 2018.
The test agents and dosages are detailed in table 7. A blank control was also set, with 4 replicates per treatment, 20m per cell2The cells are randomly arranged. The spraying agent is uniformly sprayed on the front and back surfaces of the blade by a conventional spraying method.
The investigation and statistical method comprises the following steps: the disease condition base was investigated before the administration of the drug and 7 days after the administration of the drug. Samples were taken at 5 points on the diagonal of the cell, 1 leaf per point and all leaves per plant. The grading method comprises the following steps:
level 0: no disease spots;
level 1: the lesion area accounts for less than 5% of the whole leaf area;
and 3, level: the lesion area accounts for 6 to 15 percent of the whole leaf area;
and 5, stage: the lesion area accounts for 16 to 25 percent of the whole leaf area;
and 7, stage: the lesion area accounts for 26-50% of the whole leaf area;
and 9, stage: the lesion area accounts for more than 51 percent of the whole leaf area;
disease index [ Σ (each stage of diseased leaf number × relative stage number)/(total investigated leaf number × 9) ] × 100
The prevention and treatment effect (%) is [1- (disease index before control drug application x disease index after treatment drug application)/(disease index after control drug application x disease index before treatment drug application ] × 100%
And (3) test results:
TABLE 7 prevention and treatment of powdery mildew of strawberry by mixing trifloxystrobin and metrafenone (III)
The field pesticide effect shows that (see table 7), 20% of trifloxystrobin-metrafenone missible oil, 20% of trifloxystrobin-metrafenone suspending agent, 20% of trifloxystrobin-metrafenone microemulsion, 30% of trifloxystrobin-metrafenone suspending agent and 30% of trifloxystrobin-metrafenone missible oil have good control effect on strawberry powdery mildew, and the control effect is over 80% and is obviously higher than that of a single agent.
TABLE 8 original data (III) of control effect of trifloxystrobin and metrafenone on powdery mildew of strawberry
Example 22: trifloxystrobin and metrafenone mixed for preventing and treating powdery mildew of pumpkin
Test site: huizhou city Hui city Maan, Guangdong province.
Test agents: 20% trifloxystrobin metrafenone emulsifiable concentrate, 20% trifloxystrobin metrafenone suspending agent, 20% trifloxystrobin metrafenone microemulsion, 30% trifloxystrobin metrafenone suspending agent, 30% trifloxystrobin metrafenone emulsifiable concentrate, 30% trifloxystrobin metrafenone aqueous emulsion, 40% trifloxystrobin metrafenone suspending agent, 40% trifloxystrobin metrafenone aqueous emulsion, 25% trifloxystrobin suspending agent, 40% metrafenone suspending agent and clear water control.
Test targets: powdery mildew of pumpkin.
The test agents and dosages are detailed in table 9. A blank control was also set, with 4 replicates per treatment, 20m per cell2The cells are randomly arranged. The spraying agent is uniformly sprayed on the front and back surfaces of the blade by a conventional spraying method.
The investigation and statistical method comprises the following steps: the disease condition base is investigated before drug application, the drug application is carried out 11 months and 17 days in 2018, the drug application is carried out twice after 7 days, and the disease condition base is investigated 7 days after the 2 nd drug application. Samples were taken at 5 points on the diagonal of the cell, 1 leaf per point and all leaves per plant. The grading method comprises the following steps:
level 0: no disease spots;
level 1: the lesion area accounts for less than 5% of the whole leaf area;
and 3, level: the lesion area accounts for 6 to 15 percent of the whole leaf area;
and 5, stage: the lesion area accounts for 16 to 25 percent of the whole leaf area;
and 7, stage: the lesion area accounts for 26-50% of the whole leaf area;
and 9, stage: the lesion area accounts for more than 51 percent of the whole leaf area;
disease index [ Σ (each stage of diseased leaf number × relative stage number)/(total investigated leaf number × 9) ] × 100
The prevention and treatment effect (%) is [1- (disease index before control drug application x disease index after treatment drug application)/(disease index after control drug application x disease index before treatment drug application ] × 100%
And (3) test results:
table 9: trifloxystrobin and metrafenone mixed test effect for preventing and treating powdery mildew of pumpkin (Huizhou)
The field pesticide effect shows that (see table 9), 20% trifloxystrobin-metrafenone emulsifiable solution, 20% trifloxystrobin-metrafenone suspending agent, 20% trifloxystrobin-metrafenone microemulsion, 30% trifloxystrobin-metrafenone suspending agent, 30% trifloxystrobin-metrafenone emulsifiable solution, 30% trifloxystrobin-metrafenone aqueous emulsion, 40% trifloxystrobin-metrafenone suspending agent and 40% trifloxystrobin-metrafenone aqueous emulsion have good control effect on powdery mildew of cucurbita pepo, the control effect is over 80%, and is obviously higher than that of a single agent.
TABLE 10 original control effect data of trifloxystrobin and metrafenone mixed on powdery mildew of Cucurbita pepo L (Huizhou)
Example 23: trifloxystrobin and metrafenone mixed for preventing and treating powdery mildew of bitter gourd
Test site: hainan triline.
Test agents: 25% of trifloxystrobin suspending agent, 40% of metrafenone suspending agent, 20% of trifloxystrobin metrafenone emulsifiable concentrate, 20% of trifloxystrobin metrafenone microemulsion, 30% of trifloxystrobin metrafenone suspending agent, 30% of trifloxystrobin metrafenone emulsifiable concentrate, 25% of trifloxystrobin suspending agent, 40% of metrafenone suspending agent and clear water control.
Test targets: powdery mildew of balsam pear.
The test agents and dosages are detailed in table 7. A blank control was also set, with 4 replicates per treatment, 20m per cell2The cells are randomly arranged. The spraying agent is uniformly sprayed on the front and back surfaces of the blade by a conventional spraying method.
The investigation and statistical method comprises the following steps: the disease condition base is investigated before drug application, the drug application is carried out 3 months and 7 days in 2019, the drug application is carried out twice after 7 days, and the disease condition base is investigated 7 days after the 2 nd drug application. Sampling is carried out at 5 points on the diagonal of each cell, 25 branches are investigated at each point, and all leaves are investigated by each branch. The grading method comprises the following steps:
level 0: no disease spots;
level 1: the lesion area accounts for less than 5% of the whole leaf area;
and 3, level: the lesion area accounts for 6 to 15 percent of the whole leaf area;
and 5, stage: the lesion area accounts for 16 to 25 percent of the whole leaf area;
and 7, stage: the lesion area accounts for 26-50% of the whole leaf area;
and 9, stage: the lesion area accounts for more than 51 percent of the whole leaf area;
disease index [ Σ (each stage of diseased leaf number × relative stage number)/(total investigated leaf number × 9) ] × 100
The prevention and treatment effect (%) is [1- (disease index before control drug application x disease index after treatment drug application)/(disease index after control drug application x disease index before treatment drug application ] × 100%
And (3) test results:
TABLE 11 test results of the combination of trifloxystrobin and metrafenone for the control of powdery mildew of balsam pear
The field pesticide effect shows that (see table 11), 5% of trifloxystrobin suspending agent, 40% of metrafenone suspending agent, 20% of trifloxystrobin metrafenone emulsifiable concentrate, 20% of trifloxystrobin metrafenone microemulsion, 30% of trifloxystrobin metrafenone suspending agent and 30% of trifloxystrobin metrafenone emulsifiable concentrate have good control effect on powdery mildew of balsam pear, the control effect is over 80%, and the control effect is obviously higher than that of a single agent.
TABLE 12 original data of the test effect of trifloxystrobin and metrafenone mixed for preventing and treating powdery mildew of balsam pear
Claims (10)
1. The bactericidal composition containing trifloxystrobin is characterized by comprising trifloxystrobin and metrafenone.
2. The bactericidal composition of claim 1, wherein the weight ratio of trifloxystrobin to metrafenone is 1: 80-80: 1.
3. The bactericidal composition of claim 1, wherein the weight ratio of trifloxystrobin to metrafenone is 1: 50-50: 1.
4. The bactericidal composition of claim 1, wherein the weight ratio of trifloxystrobin to metrafenone is 1: 20-20: 1.
5. The bactericidal composition of claim 1, wherein the weight ratio of trifloxystrobin to metrafenone is 1: 3-3: 1.
6. The bactericidal composition of claim 1, wherein the sum of the weights of trifloxystrobin and metrafenone accounts for 1-90%, preferably 10-60% of the composition.
7. The bactericidal composition of claim 1, wherein the composition is formulated in any form suitable for agricultural use.
8. The bactericidal composition of claim 7, wherein the composition is formulated into an agriculturally acceptable formulation, including any one of powders, dispersible tablets, granules, soluble powders, soluble granules, soluble tablets, emulsion powders, emulsion granules, water dispersible granules, wettable powders, microcapsule granules, powders, macrogranules, water dispersible tablets, microcapsule suspensions, dispersible solutions, emulsifiable concentrates, emulsion granules, oil emulsions, emulsion powders, aqueous emulsions, microemulsions, oil dispersible powders, suspensions, suspoemulsions, soluble solutions, ultra-low volume liquids, suspended seed coatings, dry seed treatment powders, dispersible seed treatment powders, seed treatment suspensions, seed treatment liquids; the preferable preparation formulation is wettable powder, water dispersible granules, suspending agents, micro-emulsions, missible oil and aqueous emulsion.
9. Use of the fungicidal composition according to any one of claims 1 to 8 for controlling crop diseases; preferably, the disease is powdery mildew; preferably, the crop is cucumber and/or strawberry; preferably, the crop diseases are cucumber powdery mildew and/or strawberry powdery mildew.
10. A method for controlling crop diseases, characterized in that the fungicidal composition according to any one of claims 1 to 8 is applied to the growing area and/or foliage and/or stem of a crop; preferably, the disease is powdery mildew; preferably, the crop is cucumber and/or strawberry; preferably, the crop diseases are cucumber powdery mildew and/or strawberry powdery mildew.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000072677A1 (en) * | 1999-05-26 | 2000-12-07 | Bayer Aktiengesellschaft | Fungicidal compositions |
| CN103651406A (en) * | 2013-12-18 | 2014-03-26 | 李视辉 | Sterilization composition containing famoxadone and trifloxystrobin and application |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000072677A1 (en) * | 1999-05-26 | 2000-12-07 | Bayer Aktiengesellschaft | Fungicidal compositions |
| CN103651406A (en) * | 2013-12-18 | 2014-03-26 | 李视辉 | Sterilization composition containing famoxadone and trifloxystrobin and application |
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