Embodiment
In order to understand better essence of the present invention, be described further by following examples, but the present invention is not limited to these embodiment.In these embodiments, unless otherwise indicated, all percentages all are weight percentage.
FORMULATION EXAMPLE 1 (the 66% phonetic phenol thiophanate-methyl of second effervescent tablet)
The phonetic phenol 60% of second
Thiophanate-methyl 6%
Sodium butylnaphthalenesulfonate (dispersant) 5%
Calcium dodecyl benzene sulfonate (wetting agent) 3%
Polyvinyl alcohol (thickener) 3%
Sodium bicarbonate (gas-producing disintegrant) 10%
Anhydrous citric acid (gas-producing disintegrant) 5%
Microcrystalline cellulose (dry binder) is supplemented to 100%
Above-mentioned formula Central Plains medicine, auxiliary agent are crushed to certain fineness through cracker; Make the 15% phonetic phenol thiophanate-methyl of second effervescent tablet after the tablet press machine compressing tablet.
This embodiment is used for preventing and treating powdery mildew of cucumber, and the 66% phonetic phenol thiophanate-methyl of second effervescent tablet is watered 2500 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 90.4%, 93.1%.
The single dose 25% phonetic phenol suspending agent of second is watered 1000 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 83.2%, 85.6%.
Single dose 70% thiophanate-methyl wetting powder is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 71.6%, 80.9%.
The 66% phonetic phenol thiophanate-methyl of second effervescent tablet is prevented and treated the powdery mildew of cucumber successful and is better than the 25% phonetic phenol suspending agent of second and 70% thiophanate-methyl wetting powder, illustrate that the phonetic phenol of second of the present invention and thiophanate-methyl composition are better than single dose, improve actual control efficiency, reduce dosage, reduce costs, delay the generation of disease drug resistance.
FORMULATION EXAMPLE 2 (the 60% phonetic phenol thiophanate-methyl of second oil-suspending agent)
The phonetic phenol 50% of second
Thiophanate-methyl 10%
Fatty alcohol-polyoxyethylene ether (emulsifier) 4%
Sodium metnylene bis-naphthalene sulfonate (dispersant) 5%
Triphenyl vinyl phenol polyoxyethylene poly-oxygen propylene aether (wetting agent) 3%
Epoxidized soybean oil (defoamer) 1%
Glycerine (antifreezing agent) 0.5%
Methylated vegetable oil (carrier) is supplemented to 100%
Above-mentioned formula is added in mixing kettle in proportion successively, namely make the 60% phonetic phenol thiophanate-methyl of second oil-suspending agent after stirring.
This embodiment is used for the control wheat powdery mildew, and the 60% phonetic phenol thiophanate-methyl of second oil-suspending agent is watered 2500 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 87.3%, 96.4%.
The single dose 25% phonetic phenol suspending agent of second is watered 1000 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 81.7%, 84.5%.
Single dose 70% thiophanate-methyl wetting powder is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 78.2%, 80.1%.
The 60% phonetic phenol thiophanate-methyl of second oil-suspending agent control wheat powdery mildew successful is better than the 25% phonetic phenol suspending agent of second and 70% thiophanate-methyl wetting powder, illustrate that the phonetic phenol of second of the present invention and thiophanate-methyl composition are better than single dose, improve actual control efficiency, reduce dosage, reduce costs, delay the generation of disease drug resistance.
FORMULATION EXAMPLE 3 (the 70% phonetic phenol thiophanate-methyl of second wetting powder)
The phonetic phenol 20% of second
Thiophanate-methyl 50%
Sodium butylnaphthalenesulfonate (wetting agent) 3%
Naphthalenesulfonate formaldehyde condensation compound (dispersant) 10%
White carbon (filler) 5%
Kaolin (filler) is supplemented to 100%.
Above-mentioned formula is entered in blender after coarse crushing in proportion mix, more namely make the 70% phonetic phenol thiophanate-methyl of second wetting powder after air-flow crushing.
This embodiment is used for control apple tree powdery mildew, and the 70% wettable mealiness agent of the phonetic phenol thiophanate-methyl of second is watered 1000 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 94.1%, 95.2%.
The single dose 25% phonetic phenol suspending agent of second is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 80.2%, 86.6%.
Single dose 70% thiophanate-methyl wettable powder disease is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 84.3%, 86.0%.
The 70% phonetic phenol thiophanate-methyl of second wetting powder control apple tree powdery mildew successful is better than the 25% phonetic phenol suspending agent of second and 70% thiophanate-methyl wetting powder, illustrate that the phonetic phenol of second of the present invention and thiophanate-methyl composition are better than single dose, improve actual control efficiency, reduce dosage, reduce costs, delay the generation of disease drug resistance.
FORMULATION EXAMPLE 4 (the 66% phonetic phenol thiophanate-methyl of second water dispersible granules)
The phonetic phenol 6% of second
Thiophanate-methyl 60%
Lauryl sodium sulfate (wetting agent) 6%
Polycarboxylate (dispersant) 8%
Sodium chloride (disintegrant) 2%
Polyethylene glycol (binding agent) 3%
Soluble starch (stabilizing agent) 3%
Bentonite (filler) is supplemented to 100%.
Dry pulverization process, granulation, drying, screening make the 30% phonetic phenol Thiophthanate methyl water dispersible granular agent of second in proportion with above-mentioned formula.
This embodiment is used for the control powdery mildew of strawberry, and the 66% phonetic phenol thiophanate-methyl of second water dispersible granules is watered 1000 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 86.7%, 90.3%.
The single dose 25% phonetic phenol suspending agent of second is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 79.1%, 82.1%.
Single dose 70% thiophanate-methyl wetting powder is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 68.6%, 72.0%.
The 66% phonetic phenol thiophanate-methyl of second water dispersible granules control powdery mildew of strawberry successful is better than the 25% phonetic phenol suspending agent of second and 70% thiophanate-methyl wetting powder, illustrate that the phonetic phenol of second of the present invention and thiophanate-methyl composition are better than single dose, improve actual control efficiency, reduce dosage, reduce costs, delay the generation of disease drug resistance.
FORMULATION EXAMPLE 5 (the 64% phonetic phenol thiophanate-methyl of second wetting powder)
The phonetic phenol 4% of second
Thiophanate-methyl 60%
Sodium butylnaphthalenesulfonate (wetting agent) 3%
Naphthalenesulfonate formaldehyde condensation compound (dispersant) 10%
White carbon (filler) 10%
Kaolin (filler) is supplemented to 100%
Above-mentioned formula is entered in blender after coarse crushing in proportion mix, more namely make the 24% phonetic phenol thiophanate-methyl of second wetting powder after air-flow crushing.
This embodiment is used for the control grape powdery mildew, and the 64% wettable mealiness agent of the phonetic phenol thiophanate-methyl of second is watered 1000 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 96.0%, 97.8%.
The single dose 25% phonetic phenol suspending agent of second is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 88.0%, 88.2%.
Single dose 70% thiophanate-methyl wettable powder disease is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 65.1%, 70.6%.
The 64% phonetic phenol thiophanate-methyl of second wetting powder control grape powdery mildew successful is better than the 25% phonetic phenol suspending agent of second and 70% thiophanate-methyl wetting powder, illustrate that the phonetic phenol of second of the present invention and thiophanate-methyl composition are better than single dose, improve actual control efficiency, reduce dosage, reduce costs, delay the generation of disease drug resistance.
FORMULATION EXAMPLE 6 (the 63% phonetic phenol thiophanate-methyl of second aqueous suspension agent)
The phonetic phenol 3% of second
Thiophanate-methyl 60%
Triphenyl vinyl phenol polyoxyethylene poly-oxygen propylene aether (emulsifier) 12%
Calcium dodecyl benzene sulfonate (wetting agent) 5%
Alkylphenol polyoxyethylene formaldehyde condensation products sulphate (dispersant) 3%
Ethylene glycol (antifreezing agent) 0.2%
Xanthan gum (stabilizing agent) 0.1%
Organosilicon (defoamer) 0.4%
Gum Arabic (thickener) 2%
Deionized water (medium) is supplemented to 100%
Above-mentioned formula is pulverized in proportion in advance, then added in sand mill and grind, allotment making the 63% phonetic phenol thiophanate-methyl of second aqueous suspension agent after high shear mixing.
This embodiment is used for control pear tree powdery mildew, and the 63% phonetic phenol thiophanate-methyl of second aqueous suspension agent is watered 1200 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 90.9%, 93.5%.
The single dose 25% phonetic phenol aqueous suspension agent of second is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 73.5%, 78.4%.
Single dose 70% thiophanate-methyl wetting powder is watered 800 times of sprayings of dilution, and after medicine, the preventive effect of 7 days, 15 days is respectively 84.1%, 86.9%.
The 63% phonetic phenol thiophanate-methyl of second aqueous suspension agent control pear tree powdery mildew successful is better than the 25% phonetic phenol suspending agent of second and 70% thiophanate-methyl wetting powder, illustrate that the phonetic phenol of second of the present invention and thiophanate-methyl composition are better than single dose, improve actual control efficiency, reduce dosage, reduce costs, delay the generation of disease drug resistance.
Biological activity determination embodiment: the phonetic phenol of second and thiophanate-methyl are mixed the apple mildew bacterium are screened
Be mixed to the co-toxicity of germ for further understanding the phonetic phenol of second and thiophanate-methyl, the inventor is mixed to the phonetic phenol of second and thiophanate-methyl and has carried out a large amount of formulation screening tests.
to be mixed etc. for the examination 95.5% former medicine of the phonetic phenol of second and 98% thiophanate methyl raw pesticide and 6 phonetic phenol of second and thiophanate-methyl, (6 phonetic phenol of second and thiophanate-methyl mixed ratio are respectively 8 medicaments: the 10% phonetic phenol of second+60% thiophanate-methyl, the 20% phonetic phenol of second+50% thiophanate-methyl, the 30% phonetic phenol of second+40% thiophanate-methyl, the 40% phonetic phenol of second+30% thiophanate-methyl, the 50% phonetic phenol of second+20% thiophanate-methyl, the 60% phonetic phenol of second+10% thiophanate-methyl), add in the sterilization triangular flask that has added in advance 2ml ethanol, fully vibration makes its dissolving, then add sterile water to 600ml, make each 600ml of 100ug/ml test mother liquor standby.On the basis of preliminary experiment, the mother liquor for preparing is diluted to 5 concentration gradients by concentration is high by low respectively, be followed successively by 100ug/g, 50ug/g, 25ug/g, 12.5ug/g, 6.25ug/g.Adopt field apple tree cauline leaf spray-on process dispenser, every 10 days spray medicines once, connect and spray 2 times.20 blades of a uniform annotinous branch of morbidity, 3 repetitions are selected in each processing.After the 2nd spray medicine 14 days, each at different levels sick number of sheets of processing of investigation respectively.
Sick leaf grade scales at different levels:
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%~15% of whole leaf area;
5 grades: lesion area accounts for 16%~25% of whole leaf area;
7 grades: lesion area accounts for 26%~50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
According to survey data, calculate disease index and control efficiency that each is processed, computing formula is as follows:
When carrying out the medicament joint toxicity measuring, this test adopts the Wadley method to calculate the coefficient of synergism (SR) of mixture, estimates the synergy type of mixture.
The Wadley method: estimate the mixed synergistic effect of mixture according to coefficient of synergism (SR), namely SR<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 are synergy.Coefficient of synergism (SR) by formula (1), (2) calculates:
In formula:
X1 ... mixture EC
50Theoretical value, unit is mg/litre (mg/L);
P
AThe percentage composition of A in mixture, unit are percentage (%);
P
BThe percentage composition of B in mixture, unit are percentage (%);
A ... the EC of A in mixture
50Value, unit is mg/litre (mg/L);
B ... the EC of B in mixture
50Value, unit is mg/litre (mg/L).
In formula:
SR ... the coefficient of synergism of mixture;
X1 ... mixture EC
50Theoretical value, unit is mg/litre (mg/L);
X2 ... mixture EC
50Measured value, unit are mg/litre (mg/L).
Record the former medicine of the phonetic phenol of second and thiophanate methyl raw pesticide and both in 6 the different blended combo close inhibition to the apple mildew bacterium, then take the logarithm value (X) of drug concentration as independent variable, take the probit value (Y) of inhibiting rate as dependent variable, adopt method of least squares to obtain virulence regression equation and the EC of each medicament
50And correlation coefficient r value.Press Wadley method (1945,1967) and obtain each combination synergism value SR (seeing attached list 1) that is mixed.
Result shows, the co-toxicity that 6 different blended combos are closed inhibition to apple mildew all has in various degree increase, the phonetic phenol of second and thiophanate-methyl to be mixed to germ shows as synergistic effect.Therefore, the inventor thinks that it is scientific and reasonable that the phonetic phenol of second and thiophanate-methyl are mixed.
Table 1, the phonetic phenol of second and thiophanate-methyl and the different proportioning toxicity test result to the apple tree powdery mildew
Reagent agent |
Virulence regression equation y=a+bx |
The r value |
EC
50(mg/L)
|
The SR value |
The former medicine of the phonetic phenol of 95.5% second |
3.7550+1.3218x |
0.9995 |
8.45 |
- |
98% thiophanate methyl raw pesticide |
3.9085+1.0712x |
0.9989 |
10.44 |
- |
The phonetic phenol of 10% second+60% thiophanate-methyl |
3.8643+1.2673x |
0.9985 |
7.87 |
1.58 |
The phonetic phenol of 20% second+50% thiophanate-methyl |
3.9520+1.3036x |
0.9839 |
6.37 |
1.53 |
The phonetic phenol of 30% second+40% thiophanate-methyl |
3.8410+1.2353x |
0.9858 |
8.67 |
1.09 |
The phonetic phenol of 40% second+30% thiophanate-methyl |
3.9966+1.1251x |
0.9986 |
7.79 |
1.18 |
The phonetic phenol of 50% second+20% thiophanate-methyl |
3.7406+1.3234x |
0.9957 |
8.95 |
1.50 |
The phonetic phenol of 60% second+10% thiophanate-methyl |
3.9706+1.2175x |
0.9976 |
8.21 |
1.02 |