CN112051250B - Medical fluorescence imaging image light supplement adjusting system and adjusting method - Google Patents
Medical fluorescence imaging image light supplement adjusting system and adjusting method Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/60—Control of cameras or camera modules
- H04N23/67—Focus control based on electronic image sensor signals
- H04N23/675—Focus control based on electronic image sensor signals comprising setting of focusing regions
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/70—Circuitry for compensating brightness variation in the scene
- H04N23/74—Circuitry for compensating brightness variation in the scene by influencing the scene brightness using illuminating means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
Abstract
The embodiment of the invention discloses a system and a method for adjusting supplementary lighting of medical fluorescence imaging images, and belongs to the technical field of medical fluorescence imaging. The medical fluorescence imaging image light supplementing adjusting system comprises a light adjusting module for adjusting the light intensity of image shooting and a shooting adjusting module for focusing and shooting a fluorescence area according to an initial shooting image; the shooting adjusting module comprises a fluorescence query module and a focusing management module; and the fluorescence query module is used for searching and positioning the fluorescence area through the influence of the shot initial fluorescence. According to the invention, the zooming adjustment work of the shooting focal length is realized by positioning the fluorescence area in the initial image and measuring the size information and the center coordinate point information of the fluorescence area, so that after the fluorescence area in the initial image is refocused, the light supplementing accurate shooting work is carried out on the fluorescence area through an automatic light supplementing means.
Description
Technical Field
The embodiment of the invention relates to the technical field of medical fluorescence imaging, in particular to a system and a method for adjusting supplementary lighting of medical fluorescence imaging images.
Background
The intensity of the fluorescent signal emitted after the fluorescent substance is excited is in a linear relation with the quantity of fluorescein in a certain range, which is the theoretical basis of the application of a fluorescent imaging system in biological research, and the performance indexes of a laser scanning system mainly comprise: system resolution, linear range, uniformity, sensitivity.
In the existing fluorescent imaging shooting process, the shooting means is usually a manual focal length adjustment means to acquire the image information of a fluorescent region, and the mode is easy to cause errors in the manual adjustment process, so that the imaging effect is poor, and the fluorescent region definition after the image shooting is easy.
Based on the above, the invention designs a system and a method for adjusting the supplementary lighting of the medical fluorescence imaging image, so as to solve the above problems.
Disclosure of Invention
The embodiment of the invention provides a system and a method for adjusting supplementary lighting of a medical fluorescence imaging image, and aims to solve the technical problems in the background art.
The embodiment of the invention provides a medical fluorescence imaging image light supplement adjusting system. In one feasible scheme, the system comprises a light adjusting module for adjusting the shooting light intensity of an image and a shooting adjusting module for focusing and shooting a fluorescent area according to an initial shooting image; the shooting adjusting module comprises a fluorescence query module and a focusing management module;
the fluorescence query module is used for searching and positioning the fluorescence area through the influence of the shot initial fluorescence;
and the focusing management module is used for acquiring the positioned fluorescence area and carrying out focusing shooting on the fluorescence area.
The embodiment of the invention provides a medical fluorescence imaging image light supplement adjusting system. In a feasible scheme, the light adjusting module comprises a light intensity control module, a light intensity signal storage module and a light supplementing time control module;
the light intensity control module is used for receiving the light intensity adjusting signal sent by the light intensity signal storage module to carry out light intensity supplementary lighting adjustment on the lamplight;
the light intensity signal storage module is used for storing light intensity control signals corresponding to all focusing data and automatically outputting the light intensity control signals to the light intensity control module after receiving the focusing data of the focusing management module;
and the light supplementing time control module is used for adjusting the light intensity control time of the light intensity control module.
The embodiment of the invention provides a medical fluorescence imaging image light supplement adjusting system. In one possible scheme, the fluorescence query module comprises an image data acquisition module, a fluorescence data comparison module and a fluorescence data storage library module;
the image data acquisition module is used for acquiring initial fluorescence imaging image data information;
the fluorescence data storage library module is used for storing fluorescence area contrast image big data information;
and the fluorescence data comparison module is used for comparing the acquired initial image data with the comparison fluorescence image and outputting fluorescence area data on the initial image reaching the specified similarity.
The embodiment of the invention provides a medical fluorescence imaging image light supplement adjusting system. In one possible scheme, the focusing management module comprises a fluorescence area measuring module, a focal length regulating module and an image shooting module;
the fluorescence area measuring module is used for measuring the acquired coordinate point information and size information of the fluorescence area;
the focal length regulating and controlling module is used for regulating the camera focal length according to the measured coordinate point information and the measured size information of the fluorescence area;
and the image shooting module is used for shooting the light supplement of the fluorescent area through the light supplement regulation and control module after the focal length is adjusted.
The embodiment of the invention provides a medical fluorescence imaging image light supplement adjusting system. In one possible approach, the fluorescence area determination module comprises an area size measurement module and an area center coordinate determination module;
the area size measuring module is used for measuring the length and width size data information of the fluorescence image in the initial image;
and the area center coordinate measuring module is used for measuring the center coordinate point information of the fluorescence area in the image coordinates.
The embodiment of the invention provides a medical fluorescence imaging image light supplement adjusting system. In one possible scheme, the focus regulation and control module comprises an inquiry data extraction module, a focusing coefficient storage module and a focusing control module;
the query data extraction module is used for calling the measurement result data information of the fluorescence area measurement module;
the focusing coefficient storage module is used for storing focusing coefficient data corresponding to the measured result data information, and automatically calling the focusing coefficient data information corresponding to the measured result data information to the focusing control module after the query data extraction module extracts the measured result data information;
and the focusing control module is used for automatically regulating and controlling the shooting focal length according to the focusing coefficient data.
The embodiment of the invention also provides a medical fluorescence imaging image light supplement adjusting method. In one possible embodiment, the method comprises the following steps:
s1, carrying out initial shooting on the fluorescence imaging area, collecting initial image data information, comparing the initial image information with a fluorescence data storage library, and searching fluorescence image data on the initial image;
s2, measuring the central coordinate point information and the area size information of the fluorescence image on the initial image, and readjusting the shooting focal length to the clear image quality of the fluorescence area;
and S3, automatically adjusting the focal length adjusting information of the fluorescent region after the image quality of the fluorescent region is clear, searching the light supplement control information adjusted by the focal length, and performing light supplement shooting on the fluorescent region.
Based on the scheme, the method has the advantages that the zoom adjustment work of the shooting focal length is realized by positioning the fluorescence area in the initial image and measuring the size information and the center coordinate point information of the fluorescence area, so that the light supplementing accurate shooting work is carried out on the fluorescence area through an automatic light supplementing means after the fluorescence area in the initial image is refocused.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a system architecture diagram of the conditioning system of the present invention;
FIG. 2 is a system diagram of a fluorescence area measurement module according to the present invention;
FIG. 3 is a system diagram of a focus adjustment module according to the present invention;
FIG. 4 is a block flow diagram of the conditioning method of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," "circumferential," and the like are used in the indicated orientations and positional relationships based on the drawings for convenience in describing and simplifying the description, but do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the invention.
In the present invention, unless otherwise specifically stated or limited, the terms "mounted," "connected," "fixed," and the like are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; the connection can be mechanical connection, electrical connection or communication connection; either directly or indirectly through intervening media, either internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The technical solution of the present invention will be described in detail below with specific examples. The following several specific embodiments may be combined with each other, and details of the same or similar concepts or processes may not be repeated in some embodiments.
Fig. 1 to 3 are diagrams illustrating a light supplement adjusting system for a medical fluoroscopic image according to a first embodiment of the present invention, in which the light supplement adjusting system for the medical fluoroscopic image is provided; the device comprises a light adjusting module for adjusting the light intensity of image shooting and a shooting adjusting module for focusing and shooting a fluorescent area according to an initial shooting image; the shooting adjusting module comprises a fluorescence query module and a focusing management module;
the fluorescence query module is used for searching and positioning the fluorescence area through the influence of the shot initial fluorescence;
and the focusing management module is used for acquiring the positioned fluorescence area and carrying out focusing shooting on the fluorescence area.
Through the above contents, when the medical fluorescence imaging image light supplement adjusting system of the invention is used for carrying out image acquisition work of a fluorescence area on a medical fluorescence imaging area, the image shooting work can be firstly carried out on fluorescence imaging by using the camera unit, so as to obtain initial image data of fluorescence imaging, then the fluorescence query module is used for querying in the fluorescence data storage library, so as to find out a fluorescence area image on the initial image corresponding to the fluorescence area big data, the positioning information of the fluorescence area image is measured by using the focusing management module, wherein the positioning information is preferably information such as a central coordinate point of the fluorescence area image on the initial imaging image and the length and width of the fluorescence area, and the focusing control work is completed by automatically searching and querying the shot focal length coefficient through the information, and after the focal length adjustment is finished, the light intensity of the focused light is adjusted by using the light adjusting and controlling module, so that the clear shooting work of the image in the fluorescent area can be realized.
Optionally, the light adjusting module comprises a light intensity control module, a light intensity signal storage module and a light supplement time control module;
the light intensity control module is used for receiving the light intensity adjusting signal sent by the light intensity signal storage module to carry out light intensity supplementary lighting adjustment on the lamplight;
the light intensity signal storage module is used for storing light intensity control signals corresponding to all focusing data and automatically outputting the light intensity control signals to the light intensity control module after receiving the focusing data of the focusing management module;
and the light supplementing time control module is used for adjusting the light intensity control time of the light intensity control module. It should be noted that, in this embodiment, in the light adjustment process, the light adjustment signal is extracted by performing automatic light supplement control by acquiring the adjustment coefficient of the focusing management module in the shooting adjustment module, and by the light supplement control method, after the focusing is completed, the light intensity is supplemented due to the change of the light intensity, so that the definition control of the fluorescent region imaging can be realized to the greatest extent.
In addition, the fluorescence inquiry module comprises an image data acquisition module, a fluorescence data comparison module and a fluorescence data storage module;
the image data acquisition module is used for acquiring initial fluorescence imaging image data information;
the fluorescence data storage library module is used for storing fluorescence area contrast image big data information;
the fluorescence data comparison module is used for comparing the acquired initial image data with the comparison fluorescence image and outputting fluorescence area data on the initial image reaching the specified similarity; when the image information of the fluorescence area on the imaging image is determined, the fluorescence area on the initial image is found by comparing the initial image with the fluorescence database.
More specifically, the focusing management module comprises a fluorescence area measuring module, a focal length regulating module and an image shooting module;
the fluorescence area measuring module is used for measuring the acquired coordinate point information and size information of the fluorescence area;
the focal length regulating and controlling module is used for regulating the camera focal length according to the measured coordinate point information and the measured size information of the fluorescence area;
the image shooting module is used for shooting a light supplement for the fluorescent area through the light supplement regulation and control module after the focal length regulation is finished, and when the focusing operation is carried out, coordinate point information and size information of the fluorescent area are obtained by utilizing the determined position of the fluorescent area, and then the shooting focal length coefficient is regulated and controlled to be shot according to the information, so that the fluorescent area is amplified or reduced.
Further, the fluorescence area determination module comprises an area size measurement module and an area center coordinate determination module;
the area size measuring module is used for measuring the length and width size data information of the fluorescence image in the initial image;
the area center coordinate measuring module is used for measuring the center coordinate point information of the fluorescence area in the image coordinate, and the focusing work can be completed by calculating the focusing coefficient by using an optical formula through the measured size information and the center coordinate point information of the fluorescence area.
Preferably, the focal length regulating module comprises an inquiry data extraction module, a focusing coefficient storage module and a focusing control module;
the query data extraction module is used for calling the measurement result data information of the fluorescence area measurement module;
the focusing coefficient storage module is used for storing focusing coefficient data corresponding to the measured result data information, and automatically calling the focusing coefficient data information corresponding to the measured result data information to the focusing control module after the query data extraction module extracts the measured result data information;
the focusing control module is used for automatically regulating and controlling the shooting focal length according to the focusing coefficient data; when focusing is carried out, the specified focal length coefficient is found by acquiring the result data measured in the fluorescence area, and shooting regulation and control work is carried out through the focal length coefficient.
Fig. 4 is a method for adjusting a light supplement of a medical fluorescence imaging image in a second embodiment of the present invention, where the second embodiment is based on an improved scheme of the first embodiment, and the method includes the following steps:
s1, carrying out initial shooting on the fluorescence imaging area, collecting initial image data information, comparing the initial image information with a fluorescence data storage library, and searching fluorescence image data on the initial image;
s2, measuring the central coordinate point information and the area size information of the fluorescence image on the initial image, and readjusting the shooting focal length to the clear image quality of the fluorescence area;
and S3, automatically adjusting the focal length adjusting information of the fluorescent region after the image quality of the fluorescent region is clear, searching the light supplement control information adjusted by the focal length, and performing light supplement shooting on the fluorescent region.
When the medical fluorescence imaging image light supplement adjusting method is used for performing light supplement adjusting work on a fluorescence region, the fluorescence region is automatically positioned, the shooting focal length is adjusted to reach the optimal value, and the shooting brightness of the fluorescence region is improved by using a light supplement means on the premise of accurate focal length, so that clear shooting work on the fluorescence region is realized.
In the present invention, unless otherwise explicitly specified or limited, the first feature "on" or "under" the second feature may be directly contacting the first feature and the second feature or indirectly contacting the first feature and the second feature through an intermediate.
Also, a first feature "on," "above," and "over" a second feature may mean that the first feature is directly above or obliquely above the second feature, or that only the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lower level than the second feature.
In the description herein, reference to the description of the term "one embodiment," "some embodiments," "an example," "a specific example" or "some examples," or the like, means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (2)
1. An adjusting method of a medical fluorescence imaging image light supplementing adjusting system is characterized by comprising a light adjusting module used for adjusting the shooting light intensity of an image and a shooting adjusting module used for focusing and shooting a fluorescence area according to an initial shooting image; the shooting adjusting module comprises a fluorescence query module and a focusing management module;
the fluorescence query module is used for searching and positioning the fluorescence area through the influence of the shot initial fluorescence;
the focusing management module is used for acquiring the positioned fluorescence area and carrying out focusing shooting on the fluorescence area;
the light adjusting module comprises a light intensity control module, a light intensity signal storage module and a light supplementing time control module;
the light intensity control module is used for receiving the light intensity adjusting signal sent by the light intensity signal storage module to carry out light intensity supplementary lighting adjustment on the lamplight;
the light intensity signal storage module is used for storing light intensity control signals corresponding to all focusing data and automatically outputting the light intensity control signals to the light intensity control module after receiving the focusing data of the focusing management module;
the light supplementing time control module is used for adjusting the light intensity control time of the light intensity control module;
in the light adjusting process, the light adjusting signal is extracted by automatically supplementing light through acquiring an adjusting coefficient of a focusing management module in a shooting adjusting module, and after focusing is finished, the light intensity is supplemented due to the change of the light intensity, so that the definition adjustment and control of imaging of a fluorescence area are realized to the maximum extent;
the focusing management module comprises a fluorescence area measuring module, a focal length regulating module and an image shooting module;
the fluorescence area measuring module is used for measuring the acquired coordinate point information and size information of the fluorescence area;
the focal length regulating and controlling module is used for regulating the camera focal length according to the measured coordinate point information and the measured size information of the fluorescence area;
the image shooting module is used for shooting a fluorescent area through the light supplement regulation and control module after the focal length is regulated;
the fluorescence area determination module comprises an area size measurement module and an area center coordinate determination module;
the area size measuring module is used for measuring the length and width size data information of the fluorescence image in the initial image;
the area center coordinate measuring module is used for measuring the center coordinate point information of the fluorescence area in the image coordinates;
the focal length regulation and control module comprises an inquiry data extraction module, a focusing coefficient storage module and a focusing control module;
the query data extraction module is used for calling the measurement result data information of the fluorescence area measurement module;
the focusing coefficient storage module is used for storing focusing coefficient data corresponding to the measured result data information, and automatically calling the focusing coefficient data information corresponding to the measured result data information to the focusing control module after the query data extraction module extracts the measured result data information;
the focusing control module is used for automatically regulating and controlling the shooting focal length according to the focusing coefficient data;
when focusing is carried out, coordinate point information and size information of a fluorescence area are obtained by utilizing the determined position of the fluorescence area, and then shooting focal length coefficient is regulated and controlled according to the information, so that the amplification or reduction regulation work of the fluorescence area is realized;
the method comprises the following steps:
s1, carrying out initial shooting on the fluorescence imaging area, collecting initial image data information, comparing the initial image information with a fluorescence data storage library, and searching fluorescence image data on the initial image;
s2, measuring the central coordinate point information and the area size information of the fluorescence image on the initial image, and readjusting the shooting focal length to the clear image quality of the fluorescence area;
s3, automatically adjusting the focal length adjusting information of the fluorescent area after the image quality of the fluorescent area is clear, searching the light supplement control information adjusted by the focal length, and performing light supplement shooting on the fluorescent area;
when the image acquisition work of the fluorescence area is carried out, the image shooting work is carried out on the fluorescence imaging by utilizing the camera shooting unit, so as to obtain initial image data of fluorescence imaging, then utilizes fluorescence inquiry module to inquire in fluorescence data storage bank, then the fluorescence area image on the initial image corresponding to the fluorescence area big data is searched, the positioning information of the fluorescence area image is measured by utilizing the focusing management module, wherein, the positioning information is the central coordinate point of the fluorescence area image on the initial imaging image and the length and width dimensions of the fluorescence area, the information is used for automatically searching and inquiring the shot focal length coefficient to complete the focusing control work, and after the focal length adjustment is completed, the light intensity of the focused light is adjusted by the light adjusting and controlling module, so that the clear shooting work of the image of the fluorescence area can be realized.
2. The adjusting method of the medical fluorescence imaging image light supplement adjusting system according to claim 1, wherein the fluorescence query module comprises an image data acquisition module, a fluorescence data comparison module and a fluorescence data storage module;
the image data acquisition module is used for acquiring initial fluorescence imaging image data information;
the fluorescence data storage library module is used for storing fluorescence area contrast image big data information;
and the fluorescence data comparison module is used for comparing the acquired initial image data with the comparison fluorescence image and outputting fluorescence area data on the initial image reaching the specified similarity.
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