CN108693113B - Imaging method, device and system - Google Patents
Imaging method, device and system Download PDFInfo
- Publication number
- CN108693113B CN108693113B CN201710227938.0A CN201710227938A CN108693113B CN 108693113 B CN108693113 B CN 108693113B CN 201710227938 A CN201710227938 A CN 201710227938A CN 108693113 B CN108693113 B CN 108693113B
- Authority
- CN
- China
- Prior art keywords
- sample
- lens module
- light
- light intensity
- sharpness value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0162—Arrangements or apparatus for facilitating the optical investigation using microprocessors for control of a sequence of operations, e.g. test, powering, switching, processing
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Microscoopes, Condenser (AREA)
- Studio Devices (AREA)
Abstract
The invention discloses an imaging method, which is used for an optical detection system, wherein the optical detection system comprises an imaging device and a carrying platform, the imaging device comprises a lens module and a focusing module, and the method comprises the following focusing steps: emitting light onto a sample placed on a stage by using a focusing module; moving the lens module to a first set position along the optical axis; enabling the lens module to move along the optical axis from the first set position to the sample by a first set step length and judging whether the focusing module receives the light reflected by the sample; when the focusing module receives light reflected by the sample, the lens module moves to the sample along the optical axis by a second set step length smaller than the first set step length, calculates a first light intensity parameter according to the light intensity of the light received by the focusing module, and judges whether the first light intensity parameter is larger than a first set light intensity threshold value or not; and when the first light intensity parameter is greater than the first set light intensity threshold value, saving the current position of the lens module as a saving position. The imaging method is convenient to operate and can reduce the cost.
Description
Technical Field
The present invention relates to the field of optical detection, and in particular, to an imaging method, apparatus, and system.
Background
Sequencing, i.e., sequencing, includes determination of nucleic acid sequences. Sequencing platforms on the market at present comprise a first-generation sequencing platform, a second-generation sequencing platform and a third-generation sequencing platform. From a functional control perspective, the sequencing instrument includes a detection module that is used to translate and/or collect changes in information generated by biochemical reactions in the sequencing to determine the sequence. The detection module generally includes an optical detection module, a current detection module, and an acid-base (pH) detection module. The sequencing platform based on the optical detection principle performs sequence determination by analyzing and collecting detected optical signal changes in sequencing biochemical reactions.
The optical detection system with the automatic focusing module sold in the market at present is provided with a matched focusing control program, can be directly called and controlled, is convenient to use, but the automatic focusing module is not usually sold independently, and a buyer can buy the whole system together with high cost.
Disclosure of Invention
Embodiments of the present invention are directed to solving at least one of the technical problems occurring in the related art or at least providing an alternative practical solution. For this reason, the embodiments of the present invention need to provide an imaging method, an optical detection system, and a control device.
The embodiment of the invention provides an imaging method, which is used for an optical detection system, the optical detection system comprises an imaging device and a carrying platform, the imaging device comprises a lens module and a focusing module, the lens module comprises an optical axis, the carrying platform is used for bearing a sample, and the method comprises the following focusing steps: emitting light onto a sample placed on the stage by using the focusing module; moving the lens module to a first set position along the optical axis; enabling the lens module to move from the first set position to the sample along the optical axis by a first set step length and judging whether the focusing module receives the light reflected by the sample; when the focusing module receives the light reflected by the sample, the lens module moves to the sample along the optical axis by a second set step length which is smaller than the first set step length, calculates a first light intensity parameter according to the light intensity of the light received by the focusing module, and judges whether the first light intensity parameter is larger than a first set light intensity threshold value; and when the first light intensity parameter is greater than the first set light intensity threshold value, saving the current position of the lens module as a saving position.
According to the imaging method, through comparison of the first light intensity parameter and the first set light intensity threshold value, interference of light signals with very weak contrast with light reflected by the sample on focusing/focusing can be eliminated; the specific position can be rapidly determined based on the detection and judgment of the change of the optical signal, and the plane of clear imaging of the target object, namely a clear plane/a clear plane, can be further rapidly and accurately found. The imaging method is particularly suitable for equipment comprising a precise optical system, wherein a clear plane is not easy to find, such as optical detection equipment with a high-power lens. Thus, the operation is convenient and the cost can be reduced.
An optical detection system according to an embodiment of the present invention includes a control device, an imaging device, and a stage, where the imaging device includes a lens module and a focusing module, the lens module includes an optical axis, the stage is used for bearing a sample, and the control device is configured to: emitting light onto a sample placed on the stage by using the focusing module; moving the lens module to a first set position along the optical axis; enabling the lens module to move from the first set position to the sample along the optical axis by a first set step length and judging whether the focusing module receives the light reflected by the sample; when the focusing module receives the light reflected by the sample, the lens module moves to the sample along the optical axis by a second set step length which is smaller than the first set step length, calculates a first light intensity parameter according to the light intensity of the light received by the focusing module, and judges whether the first light intensity parameter is larger than a first set light intensity threshold value; and when the first light intensity parameter is greater than the first set light intensity threshold value, saving the current position of the lens module as a saving position.
According to the optical detection system, through comparison of the first light intensity parameter and the first set light intensity threshold value, interference of light signals with very weak contrast with light reflected by the sample on focusing/focusing can be eliminated; the specific position can be rapidly determined based on the detection and judgment of the change of the optical signal, and the plane of clear imaging of the target object, namely a clear plane/a clear plane, can be further rapidly and accurately found. The optical detection system is particularly suitable for equipment comprising a precise optical system, which is not easy to find a clear plane, such as optical detection equipment with a high-power lens. Thus, the operation is convenient and the cost can be reduced.
The control device for controlling imaging in the embodiment of the invention is used for an optical detection system, the optical detection system comprises an imaging device and a focusing module, and the control device comprises: a storage device for storing data, the data comprising a computer executable program; a processor configured to execute the computer-executable program, wherein executing the computer-executable program comprises performing the method of the above-described embodiments.
A computer-readable storage medium of an embodiment of the present invention stores a program for execution by a computer, and executing the program includes performing the above-described method. The computer-readable storage medium may include: read-only memory, random access memory, magnetic or optical disk, and the like.
Additional aspects and advantages of embodiments of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of embodiments of the invention.
Drawings
The above and/or additional aspects and advantages of embodiments of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
fig. 1 is a schematic flow diagram of an imaging method according to an embodiment of the present invention.
Fig. 2 is a schematic diagram illustrating a positional relationship between a lens module and a sample according to an embodiment of the present invention.
Fig. 3 is a partial structural schematic diagram of an optical detection system according to an embodiment of the present invention.
Fig. 4 is another schematic flow diagram of an imaging method according to an embodiment of the invention.
Fig. 5 is a further schematic flow diagram of an imaging method according to an embodiment of the invention.
FIG. 6 is a block schematic diagram of an optical detection system according to an embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, "connected" is to be understood in a broad sense, e.g., fixedly, detachably or integrally connected; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The following disclosure provides many different embodiments or examples for implementing different features of the invention. To simplify the disclosure of the present invention, the components and settings of a specific example are described below. Furthermore, the present invention may repeat reference numerals and/or reference letters in the various examples, which have been repeated for purposes of simplicity and clarity and do not in themselves dictate a relationship between the various embodiments and/or configurations discussed.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the invention.
In the description of the present invention, the term "constant", for example relating to distance, object distance and/or relative position, may be expressed as a change in value, value range or quantity, may be absolutely constant or may be relatively constant, and the term relatively constant is maintained within a certain deviation range or a preset acceptable range. "invariant" with respect to distance, object distance, and/or relative position is relatively invariant, unless otherwise specified.
The "sequencing" referred to in the embodiments of the present invention is similar to nucleic acid sequencing, including DNA sequencing and/or RNA sequencing, including long fragment sequencing and/or short fragment sequencing. The so-called "sequencing reaction" is the same as the sequencing reaction.
Referring to fig. 1-3, an embodiment of the invention provides an imaging method for an optical inspection system, where the optical inspection system includes an imaging device 102 and a stage, the imaging device 102 includes a lens module 104 and a focusing module 106, the lens module 104 includes an optical axis OP, and the stage is used for carrying a sample 300. The imaging method includes the following focusing steps: s11, using the focusing module 106 to emit light onto the sample 300 on the stage; s12, moving the lens module 104 to a first setting position along the optical axis OP; s13, moving the lens module 104 from the first setting position to the sample 300 along the optical axis 300 by a first setting step length and determining whether the focusing module 106 receives the light reflected by the sample 300; when the focusing module 106 receives the light reflected by the sample 300, S14, the lens module 104 moves along the optical axis OP toward the sample by a second set step smaller than the first set step, calculates a first light intensity parameter according to the light intensity of the light received by the focusing module 106, and determines whether the first light intensity parameter is greater than a first set light intensity threshold; when the first light intensity parameter is greater than the first set light intensity threshold, S15, the current position of the lens module 104 is saved as the saving position.
According to the imaging method, through comparison of the first light intensity parameter and the first set light intensity threshold value, interference of light signals with very weak contrast with light reflected by the sample 300 on focusing/focusing can be eliminated; the specific position can be rapidly determined based on the detection and judgment of the change of the optical signal, and the plane of clear imaging of the target object, namely a clear plane/a clear plane, can be further rapidly and accurately found. The imaging method is particularly suitable for equipment comprising a precise optical system, wherein a clear plane is not easy to find, such as optical detection equipment with a high-power lens. Thus, the operation is convenient and the cost can be reduced.
Specifically, referring to fig. 2, in the embodiment of the invention, the sample 300 includes a carrying device 200 and a sample 302 to be tested located on the carrying device, the sample 302 to be tested is a biomolecule, such as a nucleic acid, etc., and the lens module 104 is located above the carrying device 200. The carrier 200 has a front panel 202 and a back panel (lower panel), each having two surfaces, and a sample 302 to be tested is attached to the upper surface of the lower panel, i.e., the sample 302 to be tested is located below the lower surface 204 of the front panel 202.
In the embodiment of the invention, since the imaging device 102 is used to collect the image of the sample 302 to be measured, and the sample 302 to be measured is located below the lower surface 204 of the front panel 202 of the carrying device 200, when the focusing process starts, the lens module 104 moves to find the medium interface 204 where the sample 302 to be measured is located, so as to improve the success rate of subsequently collecting clear images by the imaging device 102. In the embodiment of the present invention, the sample 302 to be tested is a solution, the front panel 202 of the carrier 200 is glass, and the medium interface 204 between the carrier 200 and the sample 302 to be tested is the lower surface 204 of the front panel 202 of the carrier 200, i.e. the interface between the glass and the liquid. Therefore, in the embodiment of the invention, by comparing the first light intensity parameter with the first set light intensity threshold, the interference of the light signal with very weak contrast with the light reflected by the medium interface 204 on focusing can be eliminated.
In one example, the front panel 202 of the sample 302 to be tested has a thickness of 0.175 mm.
In some embodiments, the carrier 200 can be a slide, and the sample 302 to be tested is placed on the slide, or the sample 302 to be tested is clamped between two slides. In some embodiments, the carrier 200 may be a reaction device, such as a chip with a sandwich structure having a carrier panel on and under, and the sample 302 to be tested is disposed on the chip.
In some embodiments, referring to fig. 3, the imaging device 102 includes a microscope 107 and a camera 108, the lens module 104 includes an objective lens 110 of the microscope and a lens module 112 of the camera 108, the focusing module 106 can be fixed with the lens module 112 of the camera 108 by a dichroic beam splitter 114(dichroic beam splitter), and the dichroic beam splitter 114 is located between the lens module 112 of the camera 108 and the objective lens 110. The dichroic beam splitter 114 includes a dual C-shaped beam splitter (dual C-mount splitter). The dichroic beam splitter 114 reflects the light emitted from the focusing module 106 to the objective lens 110 and allows visible light to pass through and enter the camera 108 through the lens module 112 of the camera 108, as shown in fig. 3.
In the embodiment of the invention, the movement of the lens module 104 may refer to the movement of the objective lens 110, and the position of the lens module 104 may refer to the position of the objective lens 110. In other embodiments, other lenses of the lens module 104 may be selectively moved to achieve focus. In addition, the microscope 107 further includes a tube lens 111(tube lens) between the objective lens 110 and the camera 108.
In some embodiments, the stage can move the sample 200 in a plane (e.g., XY plane) perpendicular to the optical axis OP (e.g., Z axis) of the lens module 104, and/or can move the sample 300 along the optical axis OP (e.g., Z axis) of the lens module 104.
In some embodiments, the plane in which the stage drives the sample 300 to move is not perpendicular to the optical axis OP, i.e. the included angle between the moving plane of the sample and the XY plane is not 0, and the imaging method is still suitable.
In addition, the imaging device 102 can also drive the objective lens 110 to move along the optical axis OP of the lens module 104 for focusing. In some examples, the imaging device 102 drives the objective lens 110 to move using an actuator such as a stepper motor or a voice coil motor.
In some embodiments, when establishing the coordinate system, as shown in fig. 2, the positions of the objective lens 110, the stage, and the sample 300 may be set on the negative axis of the Z-axis, and the first set position may be a coordinate position on the negative axis of the Z-axis. It is understood that, in other embodiments, the relationship between the coordinate system and the camera and the objective lens 110 may be adjusted according to actual situations, and is not limited in particular.
In one example, the imaging device 102 comprises a total internal reflection fluorescence microscope, the objective lens 110 is at 60 times magnification, and the first set step size S1 is 0.01 mm. Thus, the first setting step S1 is more suitable, since S1 is too large to cross the acceptable focusing range, and S1 is too small to increase the time overhead. In one example, the second set step size S2 is 0.005 mm. It is understood that, in other examples, the first setting step size and the second setting step size may also adopt other values, and are not limited specifically herein.
When the focusing module 106 does not receive the light reflected by the sample 300, the lens module 104 is moved to the sample 300 along the optical axis OP by a first set step.
When the first light intensity parameter is less than or equal to the first set light intensity threshold, the lens module 104 is moved along the optical axis OP by a second set step length.
In certain embodiments, the optical detection system may be applied to, or comprise, a sequencing system.
In some embodiments, when the lens module 104 moves, it is determined whether the current position of the lens module 104 exceeds a second predetermined position; when the current position of the lens module 104 exceeds the second setting position, the lens module 104 is stopped to move or a focusing step is performed. Thus, the first setting position and the second setting position can limit the moving range of the lens module 104, so that the lens module 104 can stop moving when focusing is failed, thereby avoiding waste of resources or damage of equipment, or refocusing the lens module 104 when focusing is failed, and improving the automation of the imaging method.
In some embodiments, such as in a total internal reflection imaging system, the arrangement is adjusted to minimize the range of motion of the lens module 104 in order to achieve a fast media interface. For example, in the total internal reflection imaging device with a 60-fold objective lens, the moving range of the lens module 104 can be set to 200 μm ± 10 μm or [190 μm, 250 μm ] according to the optical path characteristics and empirical summary.
In some embodiments, depending on the determined range of movement and the setting of either the second set position or the first set position, another set position may be determined. In one example, the second setting position is set to reflect the lowest position of the upper surface 205 of the front panel of the apparatus 200 and then the next depth of field, and the moving range of the lens module 104 is set to 250 μm, so that the first setting position is determined. In the present example, the coordinate position corresponding to the position of the next depth of field size is a position that becomes smaller in the negative Z-axis direction.
Specifically, in the embodiment of the present invention, the movement range is one section on the negative axis of the Z axis. In one example, the first set position is nearlimit, the second set position is farlimit, and the coordinate positions corresponding to nearlimit and farlimit are both located on the negative axis of the Z-axis, where nearlimit is-6000 um, and farlimit is-6350 um. The size of the range of motion defined between nearlimit and farlimit is 350 um. Therefore, when the coordinate position corresponding to the current position of the lens module 104 is smaller than the coordinate position corresponding to the second set position, it is determined that the current position of the lens module 104 exceeds the second set position. In fig. 2, the position of farlimit is the position of the depth of field L next to the lowest position of the upper surface 205 of the front panel 202 of the reaction apparatus 200. The depth of field L is the depth of field of the lens module 104.
It should be noted that, in other embodiments, the coordinate position corresponding to the first setting position and/or the second setting position may be specifically set according to the actual situation, and is not specifically limited herein.
In some embodiments, the focusing module 106 includes a light source 116 and a light sensor 118, the light source 116 is configured to emit light onto the sample 300, and the light sensor 118 is configured to receive light reflected by the sample 300. Thus, the light emitting and receiving of the focusing module 106 can be realized.
Specifically, in the embodiment of the present invention, the light source 116 may be an infrared light source 116, and the light sensor 118 may be a photo diode (photo diode), so that the cost is low and the accuracy of the detection is high. Infrared light emitted by the light source 116 is reflected by the dichroic beamsplitter into the objective lens 110 and projected through the objective lens 110 onto the sample 300. The sample 300 may reflect the infrared light projected through the objective lens 110. In an embodiment of the present invention, when the sample 300 includes the carrier 200 and the sample 302 to be measured, the received light reflected by the sample 300 is the light reflected by the lower surface 204 of the front panel of the carrier 200.
Whether the infrared light reflected by the sample 300 can enter the objective lens 110 and be received by the light sensor 118 depends primarily on the distance between the objective lens 110 and the sample 300. Therefore, when the focusing module 106 receives the infrared light reflected by the sample 300, it can be determined that the distance between the objective lens 110 and the sample 300 is within the suitable range for optical imaging, and the distance can be used for imaging of the imaging device 102. In one example, the distance is 20-40 um.
At this time, the lens module 104 is moved by a second setting step smaller than the first setting step, so that the optical detection system can search the optimal imaging position of the lens module 104 in a smaller range.
In some embodiments, the focusing module 106 includes two light sensors 118, the two light sensors 118 are configured to receive light reflected by the sample 300, and the first light intensity parameter is an average of light intensities of the light received by the two light sensors 118. In this manner, the first light intensity parameter is calculated by the average value of the light intensities of the lights received by the two light sensors 118, so that it is more accurate to exclude the interference of the weak light signal to the focusing/focusing.
Specifically, the first light intensity parameter may be set to SUM, i.e., SUM ═ PD1+ PD2)/2, and PD1 and PD2 respectively indicate the light intensities of the light received by the two light sensors 118. In one example, the first set light intensity threshold nSum is 40.
In some embodiments, referring to fig. 4, when the first light intensity parameter is greater than the first set light intensity threshold, the method further comprises the steps of: s16, moving the lens module 104 along the optical axis OP toward the sample 300 by a third setting step smaller than the second setting step, calculating a second light intensity parameter according to the light intensity of the light received by the focusing module 106, and determining whether the second light intensity parameter is smaller than a second setting light intensity threshold;
when the second light intensity parameter is smaller than the second set light intensity threshold, S17, the current position of the lens module 104 is saved to replace the previous saved position.
In this way, by comparing the second light intensity parameter with the second set light intensity threshold, the interference of the strong reflected light signal at the non-medium interface position to focusing/focusing, such as the light signal reflected by the oil surface/air of the objective lens 110, can be eliminated.
When the second light intensity parameter is not less than the second set light intensity threshold, the lens module 104 is moved to the sample 300 along the optical axis OP by a third set step length.
In one example, the third set step S3 is 0.002 mm. It is understood that, in other examples, the third setting step may also take other values, and is not limited in particular.
In addition, the current position of the lens module 104 is saved to replace the previous saved position, so that the saved position is updated, and when the imaging device 102 performs imaging subsequently, the image acquisition is performed on the sample 300 by using the saved position after the lens module 104 is updated as a starting point.
In some embodiments, the focusing module 106 includes two light sensors 118, the two light sensors 118 are configured to receive light reflected by the sample 300, the first light intensity parameter is an average value of light intensities of the light received by the two light sensors 118, the light intensities of the light received by the two light sensors 118 have a first difference, and the second light intensity parameter is a difference between the first difference and the set compensation value. In this manner, the second light intensity parameter is calculated from the light intensities of the light received by the two light sensors 118, so that the light signal excluding the strong reflection is more accurate.
Specifically, the first light intensity parameter may be set to SUM, i.e., SUM ═ PD1+ PD2)/2, and PD1 and PD2 respectively indicate the light intensities of the light received by the two light sensors 118. In one example, the first set light intensity threshold nSum is 40. The difference may be set to err and the offset may be set to err ═ offset (PD1-PD2) -offset. In an ideal situation, the first difference value may be zero. In one example, the second set light intensity threshold value nrerr is 10 and the offset is 30.
In some embodiments, referring to fig. 5, when the second light intensity parameter is smaller than the second set light intensity threshold, the imaging method further includes the following steps:
s18, moving the lens module 104 along the optical axis OP by a fourth setting step smaller than the third setting step, and using the imaging device 102 to collect the image of the sample 300, and determining whether the sharpness value of the image collected by the imaging device 102 reaches the setting threshold;
when the sharpness value of the image reaches the set threshold value, S17, the current position of the lens module 104 is saved to replace the previous saved position. In this manner, the imaging device 102 can clearly image the sample.
In the present embodiment, the sharpness value of the image may be an evaluation value (evaluation value) of image focusing. In one embodiment, determining whether the sharpness value of the image captured by the imaging device 102 reaches a set threshold may be performed by a hill-climbing algorithm of image processing. Whether the sharpness value reaches the maximum value at the peak of the sharpness value is judged by calculating the sharpness value of the image output by the imaging device 102 when the objective lens 110 is at each position, and whether the lens module 104 reaches the position of the clear plane when the imaging device 102 images is further judged. It will be appreciated that in other embodiments, other image processing algorithms may be used to determine whether the sharpness value has reached a maximum at the peak.
When the sharpness value of the image reaches the set threshold, the current position of the lens module 104 is saved to replace the previous saved position, so that the imaging device 102 can output a clear image when the sequence measurement reaction is performed for photographing.
In some embodiments, when the lens module 104 is moved by the fourth set step length, it is determined whether a first sharpness value of a pattern corresponding to a current position of the lens module 104 is greater than a second sharpness value of an image corresponding to a previous position of the lens module 104; when the first sharpness value is greater than the second sharpness value and the sharpness difference between the first sharpness value and the second sharpness value is greater than the set difference, the lens module 104 is made to continue moving towards the sample 300 along the optical axis OP by a fourth set step length; when the first sharpness value is greater than the second sharpness value and the sharpness difference between the first sharpness value and the second sharpness value is smaller than the set difference, the lens module 104 continues to move along the optical axis OP toward the sample 300 by a fifth set step smaller than the fourth set step so that the sharpness value of the image acquired by the imaging device 102 reaches the set threshold; when the second sharpness value is greater than the first sharpness value and the sharpness difference between the second sharpness value and the first sharpness value is greater than the set difference, the lens module 104 is moved away from the sample 300 along the optical axis OP by a fourth set step length; when the second sharpness value is greater than the first sharpness value and the sharpness difference between the second sharpness value and the first sharpness value is smaller than the set difference, the lens module 104 is moved away from the sample 300 along the optical axis OP by a fifth set step length so that the sharpness value of the image acquired by the imaging device 102 reaches the set threshold. Therefore, the position of the lens module 104 corresponding to the peak of the sharpness value can be accurately found, so that the image output by the imaging device is clear.
Specifically, the fourth set step may be taken as a coarse step Z1, the fifth set step may be taken as a fine step Z2, and a coarse adjustment range Z3 may be set. The coarse adjustment range Z3 is set to stop the movement of the lens module 104 when the sharpness value of the image fails to reach the set threshold, thereby saving resources.
Taking the current position of the lens module 104 as the starting point T, the coarse adjustment range Z3 is the adjustment range, i.e. the adjustment range on the Z axis is (T, T + Z3). The lens module 104 is moved in a first direction (e.g., a direction approaching the sample 300 along the optical axis OP) by a step Z1 within a range of (T, T + Z3), and a first sharpness value R1 of an image captured by the imaging device 102 at a current position of the lens module 104 is compared with a second sharpness value R2 of an image captured by the imaging device 102 at a previous position of the lens module 104. R0 represents the set difference.
When R1> R2 and R1-R2> R0, which illustrate the sharpness value of the image being closer to the set threshold and farther from the set threshold, the lens module 104 continues to move in the first direction by the step Z1 to quickly approach the set threshold.
When R1> R2 and R1-R2< R0, which indicate that the sharpness value of the image is close to the set threshold and closer to the set threshold, the lens module 104 is moved in the first direction by a step Z2 and is moved closer to the set threshold by a smaller step.
When R2> R1 and R2-R1> R0, which indicate that the sharpness value of the image has crossed the set threshold and is farther from the set threshold, the lens module 104 is moved in a second direction opposite to the first direction (e.g., a direction away from the sample 300 along the optical axis OP) by a step Z1 to quickly approach the set threshold.
When R2> R1 and R2-R1< R0, which indicates that the sharpness value of the image has crossed the set threshold and is closer to the set threshold, the lens module 104 is moved in a second direction opposite to the first direction by a step Z2, and is closer to the set threshold by a smaller step.
In some embodiments, the fifth setting step size can be adjusted to adapt to the step size approaching the setting threshold value, which is not too large or too small. The set difference can also be adjusted according to the distance from the peak of the sharpness value.
In one example, T is 0, Z1 is 100, Z2 is 40, Z3 is 2100, and the adjustment range is (0,2100). It should be noted that the above values are measurement values used when the lens module 104 is moved during the image capturing process performed by the imaging device 102, and the measurement values are light intensity-related.
In certain embodiments, the imaging method further comprises the following step of focusing: when the lens module 104 is in the storage position, acquiring the relative position of the lens module 104 and the sample 300; when the stage drives the sample 300 to move, the movement of the lens module 104 is controlled to keep the relative position unchanged. In this way, when the imaging device 102 collects images at different positions of the sample 300, the collected images are kept clear, and tracking is realized.
Specifically, the sample 300 is tilted due to physical errors of the stage and/or the sample 300, and thus, when the stage moves the sample 300, the distance between the lens module 104 and different positions on the surface of the sample 300 may vary. Therefore, when the sample 200 moves relative to the optical axis OP of the lens module 104, the imaging position of the imaging device 102 on the sample 300 is always kept at the clear plane position. This process is called focus tracking.
The stage is used to move the sample 300, including the sample 300 along an X1 axis parallel to the X axis, and the sample 300 along a Y1 axis parallel to the Y axis, and the sample 300 along a plane X1Y1 defined by the X1 axis and the Y1 axis, and the sample 300 along a plane XY defined by the X axis and the Y axis, and the sample 300 along a plane oblique to the X axis and the Y axis.
In some embodiments, when the stage drives the sample 300 to move, it is determined whether the current position of the lens module 104 exceeds a third set position; when the current position of the lens module 104 exceeds the third setting position, the stage is used to drive the sample 300 to move along the optical axis OP and a focusing step is performed; when the moving times reach the set times and the current position of the lens module 104 still exceeds the third set position, it is determined that the focus tracking fails. Thus, the limitation of the third setting position and the moving times can make the lens module 104 refocus when the focus tracking fails.
Specifically, in the present example, the third setting position may be nPos, a coordinate position corresponding to the nPos is on a negative axis of the Z-axis, and the coordinate position corresponding to the nPos is greater than a coordinate position corresponding to the second setting position farlimiit. When the coordinate position corresponding to the current position of the lens module 104 is smaller than the coordinate position corresponding to the third setting position, it is determined that the current position of the lens module 104 exceeds the third setting position.
When it is determined that the current position of the lens module 104 exceeds the third predetermined position for the first time, refocusing is performed to adjust the position of the lens module 104 to try to successfully follow up. In the process of focusing, if the number of times of moving the lens module 104 reaches the set number of times, the current position of the lens module 104 still exceeds the third set position, and then the lens module cannot focus, and it is determined that the focusing fails, and the focus is paused and refocused to find the clear plane.
The coordinate position corresponding to the third setting position is an empirical value, and when the value is smaller than the empirical value, the image acquired by the imaging device 102 is blurred and fails to be focused. The setting times are empirical values and can be specifically set according to actual conditions.
In some embodiments, when the current position of the lens module 104 does not exceed the third set position, the relative position is determined to be unchanged. In some embodiments, the relative position includes a relative distance and a relative direction. Further, to simplify the operation, the relative position may refer to a relative distance, and the invariant relative position refers to the invariant object distance of the imaging system of the imaging device 102, so that different positions of the sample 300 can be clearly imaged by the imaging device 102.
Referring to fig. 6, an optical inspection system 100 according to an embodiment of the present invention includes a control device 101, an imaging device 102 and a stage 103, where the imaging device 102 includes a lens module 104 and a focusing module 106, the lens module 104 includes an optical axis OP, the stage 103 is used for carrying a sample 300, and the control device 101 is used for: emitting light onto a sample 300 placed on a stage 103 by means of a focusing module 106; moving the lens module 104 to a first setting position along the optical axis OP; moving the lens module 104 from the first setting position to the sample 300 along the optical axis OP by a first setting step length and determining whether the focusing module 106 receives the light reflected by the sample 300; when the focusing module 106 receives the light reflected by the sample 300, the lens module 104 moves along the optical axis OP toward the sample 300 by a second set step smaller than the first set step, calculates a first light intensity parameter according to the light intensity of the light received by the focusing module 106, and determines whether the first light intensity parameter is greater than a first set light intensity threshold; when the first light intensity parameter is greater than the first set light intensity threshold, the current position of the lens module 104 is saved as the saving position.
It should be noted that the explanation and description of the technical features and advantages of the imaging method in any of the above embodiments and examples are also applicable to the optical detection system 100 of the present embodiment, and are not detailed here to avoid redundancy.
In some embodiments, the control device 101 includes a personal computer, an embedded system, a mobile phone, a tablet computer, a notebook computer, or other device with data processing and control capabilities.
In some embodiments, the focusing module 106 includes a light source 116 and a light sensor 118, the light source 116 is configured to emit light onto the sample 300, and the light sensor 118 is configured to receive light reflected by the sample 300.
Specifically, the control device 101 can control the light source 116 to emit light, and the light sensor 118 to receive light.
In some embodiments, the focusing module 106 includes two light sensors 118, the two light sensors 118 are configured to receive light reflected by the sample 300, and the first light intensity parameter is an average of light intensities of the light received by the two light sensors 118.
In some embodiments, when the first light intensity parameter is greater than the first set light intensity threshold, the control device 101 is configured to: the lens module 104 moves to the sample 300 along the optical axis OP by a third set step length smaller than the second set step length, calculates a second light intensity parameter according to the light intensity of the light received by the focusing module 106, and determines whether the second light intensity parameter is smaller than a second set light intensity threshold;
when the second light intensity parameter is smaller than the second set light intensity threshold, the current position of the lens module 104 is saved to replace the previous saved position.
In some embodiments, the focusing module 106 includes two light sensors 118, the two light sensors 118 are configured to receive light reflected by the sample 300, the first light intensity parameter is an average value of light intensities of the light received by the two light sensors 118, the light intensities of the light received by the two light sensors 118 have a first difference, and the second light intensity parameter is a difference between the first difference and the set compensation value.
In some embodiments, when the second light intensity parameter is less than the second set light intensity threshold, the control device 101 is configured to:
enabling the lens module 104 to move along the optical axis OP by a fourth set step length smaller than the third set step length, acquiring an image of the sample by using the imaging device 102, and judging whether the sharpness value of the image acquired by the imaging device 102 reaches a set threshold value;
when the sharpness value of the image reaches the set threshold, the current position of the lens module 104 is saved to replace the previous saved position.
In some embodiments, when the lens module 104 is moved by the fourth set step, the control device 101 is configured to determine whether a first sharpness value of a pattern corresponding to a current position of the lens module 104 is greater than a second sharpness value of an image corresponding to a previous position of the lens module 104; when the first sharpness value is greater than the second sharpness value and the sharpness difference between the first sharpness value and the second sharpness value is greater than the set difference, the lens module 104 continues to move along the optical axis OP toward the sample 300 by a fourth set step length; when the first sharpness value is greater than the second sharpness value and the sharpness difference between the first sharpness value and the second sharpness value is smaller than the set difference, the lens module 104 continues to move along the optical axis OP toward the sample 300 by a fifth set step smaller than the fourth set step so that the sharpness value of the image acquired by the imaging device 102 reaches the set threshold; when the second sharpness value is greater than the first sharpness value and the sharpness difference between the second sharpness value and the first sharpness value is greater than the set difference, the lens module 104 is moved away from the sample 300 along the optical axis OP by a fourth set step length; when the second sharpness value is greater than the first sharpness value and the sharpness difference between the second sharpness value and the first sharpness value is smaller than the set difference, the lens module 104 is moved away from the sample 300 along the optical axis OP by a fifth set step length so that the sharpness value of the image acquired by the imaging device 102 reaches the set threshold.
In some embodiments, when the lens module 104 moves, the control device 101 is configured to determine whether the current position of the lens module 104 exceeds a second predetermined position; when the current position of the lens module 104 exceeds the second setting position, the lens module 104 stops moving or focusing is performed.
Specifically, when the control device 101 performs focusing, the focusing step in the method of the above embodiment may be performed.
In some embodiments, the control device 101 is configured to: determining the relative position of the lens module 104 and the sample 300 when the lens module 104 is at the storage position; when the stage 103 moves the sample 300, the lens module 104 is controlled to keep the relative position unchanged.
In some embodiments, when the stage 103 is used to drive the sample 300 to move, the control device 101 is used to determine whether the current position of the lens module 104 exceeds a third set position; when the current position of the lens module 104 exceeds the third setting position, the sample 300 is driven to move by the stage 103 and focus; when the moving frequency of the sample 300 reaches the set frequency and the current position of the lens module 104 still exceeds the third set position, it is determined that the focus tracking fails.
Referring to fig. 6, a control device 101 for controlling imaging according to an embodiment of the present invention is used in an optical detection system 100, where the optical detection system 100 includes an imaging device 102 and a stage 103, and the control device 101 includes: a storage 120 for storing data, the data comprising computer executable programs; a processor 122 for executing a computer-executable program, the executing of the computer-executable program comprising performing the method of any of the above embodiments.
A computer-readable storage medium of an embodiment of the present invention stores a program for execution by a computer, and executing the program includes performing the method of any of the above embodiments. The computer-readable storage medium may include: read-only memory, random access memory, magnetic or optical disk, and the like.
In the description herein, references to the description of the terms "one embodiment," "certain embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The logic and/or steps represented in the flowcharts or otherwise described herein, such as an ordered listing of executable instructions that can be considered to implement logical functions, can be embodied in any computer-readable storage medium for use by or in connection with an instruction execution system, apparatus, or device, such as a computer-based system, processor-containing system, or other system that can fetch the instructions from the instruction execution system, apparatus, or device and execute the instructions. For the purposes of this description, a "computer-readable storage medium" can be any means that can contain, store, communicate, propagate, or transport the program for use by or in connection with the instruction execution system, apparatus, or device. More specific examples (a non-exhaustive list) of the computer readable storage medium would include the following: an electrical connection (electronic device) having one or more wires, a portable computer diskette (magnetic device), a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disc read-only memory (CDROM). Additionally, the computer-readable storage medium could even be paper or another suitable medium upon which the program is printed, as the program can be electronically captured, via for instance optical scanning of the paper or other medium, then compiled, interpreted or otherwise processed in a suitable manner if necessary, and then stored in a computer memory.
In addition, each functional unit in the embodiments of the present invention may be integrated into one processing module, or each unit may exist alone physically, or two or more units are integrated into one module. The integrated module can be realized in a hardware mode, and can also be realized in a software functional module mode. The integrated module, if implemented in the form of a software functional module and sold or used as a stand-alone product, may also be stored in a computer readable storage medium.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and not to be construed as limiting the present invention, and those skilled in the art can make changes, modifications, substitutions and alterations to the above embodiments within the scope of the present invention.
Claims (19)
1. An imaging method is characterized in that the method is used for an optical detection system, the optical detection system comprises an imaging device and a carrying platform, the imaging device comprises a lens module and a focusing module, the lens module comprises an optical axis, the carrying platform is used for bearing a sample, the sample comprises a sample to be detected, the sample to be detected is a biomolecule, and the method comprises the following focusing steps:
emitting light onto the sample placed on the stage with the focusing module;
moving the lens module to a first set position along the optical axis;
enabling the lens module to move from the first set position to the sample along the optical axis by a first set step length and judging whether the focusing module receives the light reflected by the sample;
when the focusing module receives the light reflected by the sample, the lens module moves to the sample along the optical axis by a second set step length which is smaller than the first set step length, calculates a first light intensity parameter according to the light intensity of the light received by the focusing module, and judges whether the first light intensity parameter is larger than a first set light intensity threshold value or not so as to eliminate the interference of a weak reflected light signal which is not the sample to focusing;
when the first light intensity parameter is larger than the first set light intensity threshold value, saving the current position of the lens module as a saving position;
when the first light intensity parameter is greater than the first set light intensity threshold, the method further comprises the steps of:
enabling the lens module to move to the sample along the optical axis by a third set step length smaller than the second set step length, calculating a second light intensity parameter according to the light intensity of the light received by the focusing module, and judging whether the second light intensity parameter is smaller than a second set light intensity threshold value or not so as to eliminate the interference of a strong reflected light signal which is not the sample on focusing;
and when the second light intensity parameter is smaller than the second set light intensity threshold value, saving the current position of the lens module to replace the saved position.
2. The method of claim 1, wherein the focusing module comprises a light source for emitting the light onto the sample and a light sensor for receiving the light reflected by the sample.
3. The method of claim 1, wherein the focus module comprises two light sensors for receiving the light reflected by the sample, and the first light intensity parameter is an average of light intensities of the light received by the two light sensors.
4. The method of claim 1, wherein the focusing module comprises two light sensors for receiving the light reflected by the sample, the first intensity parameter is an average of intensities of the light received by the two light sensors, the intensities of the light received by the two light sensors have a first difference, and the second intensity parameter is a difference between the first difference and a set compensation value.
5. The method of claim 1, wherein when the second light intensity parameter is less than the second set light intensity threshold, the method further comprises the steps of:
enabling the lens module to move along the optical axis by a fourth set step length smaller than the third set step length, acquiring an image of the sample by using the imaging device, and judging whether the sharpness value of the image acquired by the imaging device reaches a set threshold value;
and when the sharpness value of the image reaches the set threshold value, saving the current position of the lens module to replace the saved position.
6. The method as claimed in claim 5, wherein when the lens module is moved by the fourth set step size, determining whether a first sharpness value of the image corresponding to a current position of the lens module is greater than a second sharpness value of the image corresponding to a previous position of the lens module;
when the first sharpness value is greater than the second sharpness value and the sharpness difference between the first sharpness value and the second sharpness value is greater than a set difference, continuing to move the lens module along the optical axis toward the sample in the fourth set step;
when the first sharpness value is larger than the second sharpness value and the sharpness difference between the first sharpness value and the second sharpness value is smaller than the set difference, the lens module is made to continue to move along the optical axis to the sample in a fifth set step smaller than the fourth set step so that the sharpness value of the image acquired by the imaging device reaches the set threshold;
moving the lens module away from the sample along the optical axis in the fourth set step when the second sharpness value is greater than the first sharpness value and a sharpness difference between the second sharpness value and the first sharpness value is greater than the set difference;
when the second sharpness value is larger than the first sharpness value and the sharpness difference between the second sharpness value and the first sharpness value is smaller than the set difference, moving the lens module away from the sample along the optical axis in the fifth set step size to enable the sharpness value of the image acquired by the imaging device to reach the set threshold value.
7. The method according to any one of claims 1-6, wherein when the lens module moves, determining whether the current position of the lens module exceeds a second set position;
and stopping moving the lens module or performing the focusing step when the current position of the lens module exceeds the second set position.
8. The method according to any one of claims 1 to 6, further comprising the following step of chasing:
when the lens module is at the storage position, determining the relative position of the lens module and the sample;
and when the sample is driven to move by the stage, controlling the movement of the lens module to keep the relative position unchanged.
9. The method of claim 8, wherein when the stage moves the sample, determining whether the current position of the lens module exceeds a third predetermined position;
when the current position of the lens module exceeds the third set position, the sample is driven to move by the carrier and the focusing step is carried out;
and when the moving times of the sample reach the set times and the current position of the lens module still exceeds the third set position, judging that the focus tracking fails.
10. The utility model provides an optical detection system, its characterized in that includes controlling means, image device and microscope carrier, image device includes the camera lens module and focuses the module, the camera lens module includes the optical axis, the microscope carrier is used for bearing the sample, the sample includes the sample that awaits measuring, the sample that awaits measuring is biomolecule, controlling means is used for:
emitting light onto a sample placed on the stage by using the focusing module;
moving the lens module to a first set position along the optical axis;
enabling the lens module to move from the first set position to the sample along the optical axis by a first set step length and judging whether the focusing module receives the light reflected by the sample;
when the focusing module receives the light reflected by the sample, the lens module moves to the sample along the optical axis by a second set step length which is smaller than the first set step length, calculates a first light intensity parameter according to the light intensity of the light received by the focusing module, and judges whether the first light intensity parameter is larger than a first set light intensity threshold value or not so as to eliminate the interference of a weak reflected light signal which is not the sample to focusing;
when the first light intensity parameter is larger than the first set light intensity threshold value, saving the current position of the lens module as a saving position;
when the first light intensity parameter is greater than the first set light intensity threshold, the control device is configured to:
enabling the lens module to move to the sample along the optical axis by a third set step length smaller than the second set step length, calculating a second light intensity parameter according to the light intensity of the light received by the focusing module, and judging whether the second light intensity parameter is smaller than a second set light intensity threshold value or not so as to eliminate the interference of a strong reflected light signal which is not the sample on focusing;
and when the second light intensity parameter is smaller than the second set light intensity threshold value, saving the current position of the lens module to replace the saved position.
11. The system of claim 10, wherein the focusing module comprises a light source for emitting the light onto the sample and a light sensor for receiving the light reflected by the sample.
12. The system of claim 10, wherein the focusing module comprises two light sensors for receiving the light reflected by the sample, and the first light intensity parameter is an average of light intensities of the light received by the two light sensors.
13. The system of claim 10, wherein the focusing module comprises two light sensors for receiving the light reflected by the sample, the first light intensity parameter is an average of light intensities of the light received by the two light sensors, the light intensities of the light received by the two light sensors have a first difference, and the second light intensity parameter is a difference between the first difference and a set compensation value.
14. The system of claim 10, wherein when the second light intensity parameter is less than the second set light intensity threshold, the control means is configured to:
enabling the lens module to move along the optical axis by a fourth set step length smaller than the third set step length, acquiring an image of the sample by using the imaging device, and judging whether the sharpness value of the image acquired by the imaging device reaches a set threshold value;
and when the sharpness value of the image reaches the set threshold value, saving the current position of the lens module to replace the saved position.
15. The system as claimed in claim 14, wherein when the lens module is moved by the fourth set step size, the control device is configured to determine whether a first sharpness value of the image corresponding to a current position of the lens module is greater than a second sharpness value of the image corresponding to a previous position of the lens module;
when the first sharpness value is greater than the second sharpness value and the sharpness difference between the first sharpness value and the second sharpness value is greater than a set difference, continuing to move the lens module along the optical axis toward the sample in the fourth set step;
when the first sharpness value is larger than the second sharpness value and the sharpness difference between the first sharpness value and the second sharpness value is smaller than the set difference, the lens module is made to continue to move along the optical axis to the sample in a fifth set step smaller than the fourth set step so that the sharpness value of the image acquired by the imaging device reaches the set threshold;
moving the lens module away from the sample along the optical axis in the fourth set step when the second sharpness value is greater than the first sharpness value and a sharpness difference between the second sharpness value and the first sharpness value is greater than the set difference;
when the second sharpness value is larger than the first sharpness value and the sharpness difference between the second sharpness value and the first sharpness value is smaller than the set difference, moving the lens module away from the sample along the optical axis in the fifth set step size to enable the sharpness value of the image acquired by the imaging device to reach the set threshold value.
16. The system according to any one of claims 11-15, wherein the control device is configured to determine whether the current position of the lens module exceeds a second predetermined position while the lens module is moving;
and when the current position of the lens module exceeds the second set position, stopping moving the lens module or focusing.
17. The system of any one of claims 11-15, wherein the control device is configured to:
when the lens module is at the storage position, determining the relative position of the lens module and the sample;
and when the sample is driven to move by the stage, controlling the movement of the lens module to keep the relative position unchanged.
18. The system of claim 17, wherein when the stage is used to move the sample, the control device is configured to determine whether a current position of the lens module exceeds a third predetermined position;
when the current position of the lens module exceeds the third set position, the sample is driven to move by the carrier and is focused;
and when the moving times of the sample reach the set times and the current position of the lens module still exceeds the third set position, judging that the focus tracking fails.
19. A control device for controlling imaging, for use in an optical inspection system comprising an imaging device and a focusing module, the control device comprising:
a storage device for storing data, the data comprising a computer executable program;
a processor for executing the computer-executable program, execution of the computer-executable program comprising performing the method of any of claims 1-9.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710227938.0A CN108693113B (en) | 2017-04-10 | 2017-04-10 | Imaging method, device and system |
| PCT/CN2018/078817 WO2018188441A1 (en) | 2017-04-10 | 2018-03-13 | Imaging method, device and system |
| PCT/CN2018/078799 WO2018188440A1 (en) | 2017-04-10 | 2018-03-13 | Imaging method, device and system |
| PCT/CN2018/078836 WO2018188442A1 (en) | 2017-04-10 | 2018-03-13 | Imaging method, device and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710227938.0A CN108693113B (en) | 2017-04-10 | 2017-04-10 | Imaging method, device and system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108693113A CN108693113A (en) | 2018-10-23 |
| CN108693113B true CN108693113B (en) | 2021-08-27 |
Family
ID=63843327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710227938.0A Active CN108693113B (en) | 2017-04-10 | 2017-04-10 | Imaging method, device and system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108693113B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101438147A (en) * | 2006-05-31 | 2009-05-20 | 奥林巴斯株式会社 | Biological specimen imaging method and biological specimen imaging apparatus |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101931967B1 (en) * | 2011-09-19 | 2018-12-27 | 삼성전자 주식회사 | Auto focusing apparatus for optical microscope |
| JP6506908B2 (en) * | 2014-02-24 | 2019-04-24 | オリンパス株式会社 | Focusing method, measuring method, focusing device, and measuring device |
-
2017
- 2017-04-10 CN CN201710227938.0A patent/CN108693113B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101438147A (en) * | 2006-05-31 | 2009-05-20 | 奥林巴斯株式会社 | Biological specimen imaging method and biological specimen imaging apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108693113A (en) | 2018-10-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108693625B (en) | Imaging method, device and system | |
| US11086118B2 (en) | Self-calibrating and directional focusing systems and methods for infinity corrected microscopes | |
| US10754135B2 (en) | Light sheet microscope and control method for light sheet microscope | |
| RU2710004C1 (en) | Device and methods for predictive tracking of focus | |
| JP2017021025A (en) | Adjustment method of adaptable operation frequency of variable focal length lens in magnification adjustable optical system | |
| CN108693624B (en) | Imaging method, device and system | |
| EP3387370B1 (en) | Focusing system for a telecentric optical measuring machine | |
| US20150358533A1 (en) | Control method for imaging apparatus and imaging system | |
| CN102122055A (en) | Laser-type automatic focusing device and focusing method thereof | |
| WO2018188440A1 (en) | Imaging method, device and system | |
| US20220279130A1 (en) | Imaging method, device and system | |
| US10721368B2 (en) | Slide rack determination system | |
| CN112322713B (en) | Imaging method, device and system and storage medium | |
| CN116540393B (en) | Automatic focusing system and method, semiconductor defect detection system and method | |
| JP4773198B2 (en) | Specimen imaging apparatus and specimen analyzer including the same | |
| CN108693113B (en) | Imaging method, device and system | |
| KR102010818B1 (en) | Apparatus for capturing images of blood cell | |
| US10288861B2 (en) | Light sheet microscope and control method of light sheet microscope | |
| CN114071133B (en) | Semiconductor chip detection image imaging method | |
| CN112291469A (en) | Imaging method, device and system | |
| CN112333378A (en) | Imaging method, device and system | |
| JP4974059B2 (en) | Auto focus control system | |
| WO2022245689A2 (en) | Automated nanoscopy system having integrated artifact minimization modules, including embedded nanometer position tracking based on phasor analysis | |
| CN117891042A (en) | Focusing method, optical imaging system, sequencing system and medium | |
| GB2580412A (en) | Sensor for precise distance measuring in automated imaging |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 518000 Shenye Jinyuan Building, No. 116 Qingshuihe Road, Qingshuihe Street, Luohu District, Shenzhen City, Guangdong Province, 2 5th and 6th floors Applicant after: Shenzhen Zhenmai Biotechnology Co., Ltd. Address before: 518000 National Supercomputing Shenzhen Center Research Building, No. 9 Tuxue Road, Xili University Town, Nanshan District, Shenzhen City, Guangdong Province, 10th Floor Applicant before: SHENZHEN HANHAI GENE BIOTECHNOLOGY CO., LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |