CN114152610A - Slide cell scanning method based on visual target marker - Google Patents
Slide cell scanning method based on visual target marker Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 70
- 230000000007 visual effect Effects 0.000 title claims abstract description 26
- 239000003550 marker Substances 0.000 title description 7
- 210000004027 cell Anatomy 0.000 claims abstract description 77
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 43
- 239000003086 colorant Substances 0.000 claims abstract description 6
- 238000012216 screening Methods 0.000 claims description 3
- 230000005859 cell recognition Effects 0.000 claims description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 4
- 230000007762 localization of cell Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
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- 238000011112 process operation Methods 0.000 description 1
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Abstract
The invention relates to the technical field of image processing, and discloses a slide cell scanning method based on visual target marks, which is characterized in that leucocyte recognition is carried out on a 20-time objective lens based on cell colors, and after the recognition is finished, the slide steps of a high-low-time lens are converted; performing region division on a picture acquired by a 20-time objective lens, and performing reset scanning and cell calibration on white blood cells by using a high-power lens; if the set number of cells are not obtained, the conventional process is switched to obtain the cells until the set number of cells are obtained, meanwhile, in order to ensure the position before shooting is determined, the starting point is debugged in advance, the problem that the conventional slide cell scanning process cannot accurately find the positions of the white blood cells, so that the whole process is overlong is solved, and the identification efficiency is ensured.
Description
Technical Field
The invention relates to the technical field of image processing, in particular to a slide cell scanning method based on a visual target mark.
Background
Conventional slide cell scanning procedure: taking pictures > moving the lens one field of view > taking pictures again, after setting the slide cell scanning path until the number of white blood cells scanned is sufficient, or the scanning process is overtime. The cell images scanned by the method are random, and the fact that each visual field has white blood cells cannot be guaranteed.
Disclosure of Invention
The invention aims to provide a slide cell scanning method based on a visual target marker, and the identification efficiency is ensured.
In order to achieve the above object, the present invention provides a method for scanning cells of a slide based on a visual target marker, comprising the following steps:
carrying out leucocyte identification on a 20-time objective lens based on cell colors, and converting the number of slide steps of a high-low time lens after the identification is finished;
performing region division on a picture acquired by a 20-time objective lens, and performing reset scanning and cell calibration on white blood cells by using a high-power lens;
and if the set number of cells are not obtained, transferring to a conventional process to obtain the cells until the set number of cells are obtained.
Wherein, carry out leucocyte discernment at 20 times objective lens based on cell colour to after discernment is accomplished, convert the slide step number of high low power mirror, include:
according to the colors of the cells and the background of the shot picture and the size of the cells, setting a threshold value to screen the cells, and recording the coordinates of the white blood cells under the objective lens with the magnification of 20 times; when the screening number reaches the scanning threshold value, ending the 20-time shooting process;
the number of slide steps of the low power mirror and the high power mirror is switched based on the set starting point.
Wherein, based on the set starting point, the step number of the slide of the low power mirror and the high power mirror is converted, which comprises the following steps:
the set position of the first block under the 20 times objective lens corresponds to the starting point under the high-power lens;
and converting the coordinates of each picture in the pictures shot by the 20-time objective lens into the motor step number according to the corresponding relation between the pixels and the visual field.
Wherein, carry out regional division with the picture that will acquire through 20 times objective lens to utilize high power mirror to carry out reset scanning and cell calibration to leucocyte, include:
dividing each block shot by a 20-time objective lens into 25 areas, and taking the area No. 1 as a block reset point;
when shooting is carried out by using a 100-time objective lens, reading the position of a white blood cell each time, starting from a block reset point, resetting to the block reset point after each shooting is finished, and moving to the next block to search for the white blood cell;
and performing cell recognition on the obtained white blood cells, calculating cell coordinates, and automatically compensating the coordinates to perform cell calibration when a 100-time objective lens is used for shooting the next block.
Wherein, before the leukocyte identification using the 20-fold objective lens, the method further comprises:
and debugging the starting point of the scanning instrument, including manual debugging and automatic debugging.
Wherein, carry out manual debugging to scanning instrument, include:
finding a starting point based on a left boundary under an objective lens of 100 times, moving to an objective lens of 20 times to find the same area, and moving to the upper left corner of a film reading area in parallel after finding the starting point based on the boundary according to the principle that the steps of motors of 100 times and 20 times are the same;
after finding the corresponding starting point, recording the movement coordinates of the 100 times starting point and the 20 times starting point x and y respectively, and taking the movement coordinates as the starting point parameters of the instrument.
According to the method for scanning the cells of the glass slide based on the visual target marker, the leucocyte is identified on the basis of the cell color by the objective lens with the power of 20 times, and the step number of the glass slide of the high-low power lens is converted after the identification is finished; performing region division on a picture acquired by a 20-time objective lens, and performing reset scanning and cell calibration on white blood cells by using a high-power lens; if the set number of cells are not obtained, the conventional process is switched to obtain the cells until the set number of cells are obtained, meanwhile, in order to ensure the position before shooting is determined, the starting point is debugged in advance, the problem that the conventional slide cell scanning process cannot accurately find the positions of the white blood cells, so that the whole process is overlong is solved, and the identification efficiency is ensured.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic step diagram of a slide cell scanning method based on a visual target mark provided by the invention.
FIG. 2 is a flow chart of the cell localization provided by the present invention.
Fig. 3 is a flow chart of the starting point debugging provided by the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 3, the present invention provides a method for scanning cells of a slide based on a visual target marker, comprising the following steps:
s101, carrying out leucocyte recognition based on cell colors at a 20-time objective lens, and converting the slide steps of the high-low time lens after the recognition is finished.
Specifically, a threshold is set for screening according to the color and size of the cells and background of the photographed image, and coordinates under a 20-fold objective lens of the white blood cells are recorded. And when the quantity to be screened is enough, ending the 20-time shooting process, and switching to the 100-time process for recognition shooting.
Because the position of the white blood cells needs to be read in the high power lens positioning process. Before the 100-time objective lens shooting process is started, coordinate conversion needs to be performed on recorded leukocyte coordinates under 20-time objective lenses, the leukocyte coordinates are converted into motor operation coordinates under the 100-time objective lens process, and then the identification process of the high-power objective lens is started, wherein the step number conversion method comprises the following steps:
the method comprises the steps of firstly, ensuring that a position 1 area of a first block under the 20-time process corresponds to an initial point under the 100-time process, and ensuring that each position under the 20-time process corresponds to each position under the 100-time process one by one based on the fact that the 20-time motor step number is the same as the 100-time motor step number after the initial point is set.
Converting the white blood cell coordinate into the motor step number: on the premise of ensuring the correspondence of the block positions, the coordinates of each picture in 20 times of shot pictures are converted into the number of motor steps according to the corresponding relation between the pixels and the visual field, and the positions of the white blood cells can be correctly found after the number of motor steps is read in the 100 times of shooting process.
And S102, carrying out region division on the picture acquired by the 20-time objective lens, and carrying out reset scanning and cell calibration on the white blood cells by using a high-power lens.
Specifically, before describing the slide step number conversion method using the high power mirror and the low power mirror, it is necessary to describe a cell localization procedure under the high power mirror and a conventional procedure in order to understand the necessity of the step number conversion method:
the conventional process comprises the following steps: shooting is directly carried out, cell positioning is not carried out, and pictures are shot randomly by continuously moving a lens;
firstly, the existence of white blood cells in every visual field cannot be guaranteed;
secondly, the white blood cells in each visual field cannot be ensured to be in the center, so that the condition of marginal cells can occur, and the identification efficiency is influenced.
The method for scanning the cells of the slide based on the visual target marker comprises the following steps: each block shot by 20 times is divided into 25 areas, the area No. 1 is a block reset point, in the shooting process of the 100-time objective lens, the position of the white blood cell is read every time, then starting from the reset point, the cell shot every time is ensured to be in the center of the visual field, the block reset point is reset after the shooting is completed every time, and the accuracy of the next slide cell scanning is ensured.
After shooting a 20-time small area, resetting to the reset point of the No. 1 area of each block, and moving to the next block to search cells.
The cell can not be accurately moved to the center of the lens due to motor errors when the picture is shot under the high-power mirror, so that the cell identification is carried out on the picture under the high-power mirror, the cell coordinate is calculated, the coordinate is automatically compensated when the next cell is shot, and the accuracy of slide cell scanning at each time is ensured.
And S103, if the set number of cells are not obtained, transferring to a conventional process to obtain the cells until the set number of cells are obtained.
Specifically, due to the uncertainty of the types of the white blood cells and the interference of smearing cells and platelets, the positioning process cannot be guaranteed to accurately identify 100 white blood cells, and the adaptability of the conventional process is wide, so that after the operation of the slide cell scanning method is finished, the number of the cells does not reach the standard, the conventional process is switched to compensate, and the adaptability of the method is improved.
Because the positions of the slides under 20 times of objective field and 100 times of objective field need to be in one-to-one correspondence in the slide step number conversion method of the high-power mirror and the low-power mirror, the starting point of the instrument needs to be set before cell scanning, and the one-to-one correspondence between the high-power mirror field and the low-power mirror field is ensured. Therefore, the starting point motor step number needs to be debugged before each instrument leaves the factory. The setting flow is as follows:
when the initial step number does not exist, the instrument cannot be automatically debugged, the instrument needs to be manually and finely debugged, and the initial step number of the starting point is obtained, and can be used as the initial step number of the instrument and also can be used as the starting point parameter of automatic debugging of the instrument later.
Usually, a starting point is found under a 100-time objective lens based on a left boundary, the objective lens is moved to a 20-time objective lens to find the same area, and the starting point based on the boundary is found and then the objective lens is moved to the upper left corner of a film reading area in parallel according to the principle that the 100-time and 20-time motor steps are the same.
And after the movement coordinates of the X and the Y of the 100 times of starting point and the 20 times of starting point are respectively recorded to be used as starting point parameters of a subsequent instrument, and the manual debugging process of the instrument is ended.
After the initial parameters are obtained, the subsequent instruments can enter an automatic debugging process by using the parameters of the first instrument:
inputting a starting point parameter: the starting point parameters obtained previously together are input into the flow.
And (3) according to the parameters, under an objective lens of 100 times, splicing into 1 large block flow according to 25 small areas, carrying out moving shooting and searching for white blood cells. And records the movement position.
After finding the white blood cells, the white blood cells are transferred to a 20-time objective lens, and coordinates are reversely converted according to a coordinate conversion principle. Leukocyte localization was obtained and moved to leukocyte location.
Since the motor of each instrument has errors and it cannot be guaranteed that the initial point can be adapted to other instruments, 9 blocks around the block position based on the leukocyte recognition area are shot under a 20-time objective lens. Since the 20-time visual field is wider than the 100-time visual field, the process can be normally carried out as long as the motor error is ensured to be within the range of 20-time 9 block visual fields.
After 9 blocks are shot, corresponding white blood cells are manually searched, corresponding block positions are selected, and compensation is carried out according to the difference between the block positions and the distance centers of the white blood cells.
And (4) after the compensation is finished, testing a flow verification result, wherein the testing flow is a running positioning flow and only identifies the condition of one leukocyte, and if the 100-time vision center in the 3 testing results has the leukocyte, the debugging is determined to be finished, and the flow is finished.
The details of the automatic flow are complicated, but manual adjustment is not needed, and the instrument adjustment can be completed by only clicking and debugging, acquiring parameters, inputting parameters and testing results and performing a few simple operations.
The manual debugging process is simple, but the initial point needs to be found manually, and the adjustment is time-consuming and labor-consuming. Therefore, an automatic debugging process is written according to the precision of the instrument. After the initial point parameters are obtained, all the instruments after being debugged by using the automatic debugging function can be used on the premise of ensuring that the mechanical precision of different instruments is within 20 times of the field of view of the 9 blocks of the objective lens, so that the initial point setting process operation becomes simple.
Advantageous effects
Slide scanning procedure with cell localization procedure added:
1. the cell scanning process and the positioning process are increased by 20 times, and the cell identification process of a high-power mirror and a low-power mirror is increased. So that each time the camera can accurately find the white blood cells.
2. The number of 100 times of slide scanning is greatly reduced, the average recognized 100 white blood cells is reduced from 1000 pictures of the conventional process to 100 pictures on average, and the slide cell scanning time is greatly reduced.
3. Because the cells are in the central position when pictures are taken every time, the cells are guaranteed to be in the leucocyte layer when the pictures are focused every time, and the definition of the images and the accuracy of cell identification during continuous shooting are guaranteed.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. A method for scanning slide cells based on visual target marks is characterized by comprising the following steps:
carrying out leucocyte identification on a 20-time objective lens based on cell colors, and converting the number of slide steps of a high-low time lens after the identification is finished;
performing region division on a picture acquired by a 20-time objective lens, and performing reset scanning and cell calibration on white blood cells by using a high-power lens;
and if the set number of cells are not obtained, transferring to a conventional process to obtain the cells until the set number of cells are obtained.
2. The method for scanning the cells of the slide based on the visual target mark according to claim 1, wherein the leucocyte recognition is carried out on the basis of the cell color at a 20-fold objective lens, and the slide steps of the high-and-low-fold lens are converted after the recognition is finished, and the method comprises the following steps:
according to the colors of the cells and the background of the shot picture and the size of the cells, setting a threshold value to screen the cells, and recording the coordinates of the white blood cells under the objective lens with the magnification of 20 times; when the screening number reaches the scanning threshold value, ending the 20-time shooting process;
the number of slide steps of the low power mirror and the high power mirror is switched based on the set starting point.
3. The method for slide cytology scanning based on visual target markers of claim 2 wherein the converting the slide steps for the low power mirror and the high power mirror based on a set starting point comprises:
the set position of the first block under the 20 times objective lens corresponds to the starting point under the high-power lens;
and converting the coordinates of each picture in the pictures shot by the 20-time objective lens into the motor step number according to the corresponding relation between the pixels and the visual field.
4. The method for scanning cells of a slide based on visual target marks according to claim 1, wherein the image obtained by a 20-fold objective lens is divided into areas, and the white blood cells are subjected to reset scanning and cell calibration by a high-fold lens, and the method comprises the following steps:
dividing each block shot by a 20-time objective lens into 25 areas, and taking the area No. 1 as a block reset point;
when shooting is carried out by using a 100-time objective lens, reading the position of a white blood cell each time, starting from a block reset point, resetting to the block reset point after each shooting is finished, and moving to the next block to search for the white blood cell;
and performing cell recognition on the obtained white blood cells, calculating cell coordinates, and automatically compensating the coordinates to perform cell calibration when a 100-time objective lens is used for shooting the next block.
5. The slide cytology scanning method based on visual target markers of claim 1, wherein prior to performing leukocyte identification using a 20-fold objective lens, the method further comprises:
and debugging the starting point of the scanning instrument, including manual debugging and automatic debugging.
6. The slide cytology scanning method based on visual target marks of claim 5, wherein manual commissioning of the scanning instrument comprises:
finding a starting point based on a left boundary under an objective lens of 100 times, moving to an objective lens of 20 times to find the same area, and moving to the upper left corner of a film reading area in parallel after finding the starting point based on the boundary according to the principle that the steps of motors of 100 times and 20 times are the same;
after finding the corresponding starting point, recording the movement coordinates of the 100 times starting point and the 20 times starting point x and y respectively, and taking the movement coordinates as the starting point parameters of the instrument.
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