CN115712194A - Portable full-automatic high-speed micro scanner - Google Patents

Abstract

The invention provides a portable full-automatic high-speed microscopic scanner, which comprises a stepping objective table, a microscope barrel and a stroboscopic head, wherein the stepping objective table is provided with a scanning head; the microscope tube is connected with the industrial camera and fixedly connected with a z-axis seat of the stepping objective table; the stepping object stage is used for carrying a glass slide; the microscope lens cone is coaxial or one side of the microscope lens cone is also provided with a stroboscopic head, the stroboscopic head is used for emitting flashes according to a set frequency according to the continuous walking stroke of the stepping object stage, and the industrial camera collects images along with the flashing frequency of the stroboscopic head. The invention adopts an image acquisition scheme based on rectangular field continuous scanning and a scheme of improving the image acquisition brightness by matching with a stroboscopic head, greatly improves the image acquisition efficiency on the premise of ensuring the image acquisition precision, and reduces the image acquisition to 60 to 120 seconds from more than 20 minutes before. The scheme of the guide seat with the self-guiding and self-driving structure is adopted, the structure of the objective table is greatly simplified, and the new walking speed is convenient to increase.

Description

Portable full-automatic high-speed micro scanner

Technical Field

The invention relates to the field of microscopic image acquisition, in particular to a portable full-automatic high-speed microscopic scanner.

Background

The cell and tissue pathology image identification technology is considered as an authoritative and definite diagnosis means, but the detection process of the cell and tissue pathology image identification technology is long and comprises the processes of sampling, flaking, sample microscopic image acquisition, image splicing, image identification and the like. In the fully artificial age, only one single-digit diagnosis can be completed by one pathologist a day. This is far from satisfying the user's needs. The existing diagnostic procedure has a bottleneck that the equipment for microscopic image scanning is expensive and time-consuming. In foreign technology, dr, weinstein, in cooperation with D Metrix research and development team in the united states, developed a set of pathological diagnosis product rapid digital slice micro-scanner DX-40. The scheme of integrating 80 microscopes for scanning simultaneously can realize the processing speed of 40 sheets per hour. But the structure is very complicated, resulting in high price. The applicant previously developed a series of miniature microscopic image acquisition devices, such as CN110794569A cell miniature microscopic image acquisition device and image recognition method. The mobile phone is used as the image acquisition equipment, so that the cost of the equipment is greatly reduced. However, various parameters of the mobile phone camera related to the equipment are different, so that images shot by each mobile phone have different visual field ranges, the splicing and recognition difficulty is higher, and the difference between the image precision of the mobile phone and the image precision of the industrial camera still exists. Moreover, the device still needs about 2 hours to complete the collection and operation of a single slide image, the efficiency is low, and the working efficiency of the existing device limits the development of the technology along with the popularization of cell and tissue pathological image recognition technology and more users. US patent US8755579A describes a fully automatic rapid microscope slide scanner that employs a scheme of continuous line scanning real-time stitching, which greatly increases the speed of acquisition. But the requirement on the precision of the equipment is extremely high, otherwise, the error of the spliced microscopic image is large, and the auxiliary diagnosis value is not available. Also, there are fewer specialized lenses on the market for linear scanners, which results in an exponential increase in the imaging quality and cost of the device.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a portable full-automatic high-speed microscopic scanner, which can greatly improve the speed of image acquisition, does not greatly increase the cost of equipment, and can ensure the quality of scanned images. In preferred embodiments, there is also a need for a more compact construction of the micro-scanners, so that more micro-scanners can be clustered to further improve scanning efficiency.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a portable full-automatic high-speed microscopic scanner comprises a stepping objective table, a microscope barrel and a stroboscopic head;

the microscope tube is connected with the industrial camera and fixedly connected with a z-axis seat of the stepping objective table;

the stepping object stage is used for carrying a glass slide;

the microscope lens cone is coaxial or one side of the microscope lens cone is also provided with a stroboscopic head, the stroboscopic head is used for emitting flashes according to a set frequency according to the continuous walking stroke of the stepping object stage, and the industrial camera collects images along with the flashing frequency of the stroboscopic head.

In a preferred embodiment, the stepping stage has a structure: the X-axis stepping motor is fixedly connected with the X-axis motor base, an X-axis arm is arranged on one side corresponding to the X-axis base, a nut is fixedly arranged on the X-axis arm, the X-axis stepping motor is fixedly connected with an X-axis screw rod, and the X-axis screw rod is in threaded connection with the nut;

a y-direction groove is formed in the top of the x-direction seat, the y-direction seat is arranged in the y-direction groove and slides along the y direction, a y-axis motor seat extending to one side of the x direction is arranged on the x-direction seat, the y-axis motor seat is fixedly connected with a y-axis stepping motor, an extending y-arm is arranged on one side of the y-direction seat corresponding to the y-axis seat, a nut is fixedly arranged on the y-arm, the y-axis stepping motor is fixedly connected with a y-axis screw rod, and the y-axis screw rod is in threaded connection with the nut;

the top of the y-direction seat is provided with a groove body facing outwards, and the slide frame is arranged in the groove body.

In the preferred scheme, an x-axis stepping motor and a y-axis stepping motor are connected with a controller, and the controller adopts open-loop control;

the controller is connected with an industrial personal computer, the industrial personal computer sends a stepping signal to the controller, and the industrial personal computer also sends a stroboscopic signal to the stroboscopic head according to the stepping signal and the corresponding visual field position;

the industrial personal computer starts the industrial camera to collect images according to the stroboscopic signal and transmits the images to the memory.

In the preferred scheme, an x-axis stroke sensor is arranged between an x-axis motor base and an x-axis arm and used for verifying the relative displacement between stroboflash and an x-direction base, and if an error is detected, the error value is sent and stored to be used as an x-direction correction value in the subsequent image splicing process;

and a y-axis stroke sensor is arranged between the y-axis motor base and the y-arm and used for verifying the relative displacement between the stroboflash of the x-axis base and the stroboflash of the y-axis base, and if an error is detected, the error value is sent and stored to be used as a y-axis correction value in the subsequent image splicing process.

In the preferred scheme, a display device is further arranged and connected with an industrial personal computer, and after the industrial personal computer receives the acquired images, the acquired images are spliced according to the scanning path and the corresponding coordinates of the visual field;

and introducing an x-direction correction value and a y-direction correction value for correction in the splicing process.

In a preferred scheme, the system is further provided with an optical compensation sensor, wherein the optical compensation sensor is used for detecting the intensity value of the stroboscopic light every time the industrial camera acquires an image, averaging the light intensity values corresponding to the fields of view, then calculating the difference value between the intensity value of the stroboscopic light of each field of view and the average value, and when the difference value exceeds a preset range, performing compensation adjustment on the brightness of the current field of view.

In a preferred scheme, the stroboscopic head is structured in such a way that an LED light source is electrically connected with an output end of a switch element, a control end of the switch element is electrically connected with a controller, and an input end of the switch element is electrically connected with a power supply;

the maximum brightness of the stroboscopic head is 50000lx to 100000 lx, the stepping speed of the stepping object stage is 17 to 20ms/visual field, the numerical aperture of the microscope tube is 0.5 to 0.75, and the exposure time of the industrial camera is 10 to 25 microseconds.

In the preferred scheme, the device is also provided with a panoramic lens, the visual field of the panoramic lens covers the whole slide frame or slide, and the panoramic lens is used for collecting a coded image and a panoramic image of an organization sample so as to obtain the information of the current slide and guide the subsequent continuous walking stroboscopic image collection operation.

In an optimal scheme, a lens barrel seat is arranged on a z-axis seat, the lens barrel seat is in sliding connection with the z-axis seat along the z direction, a microscope lens barrel and an industrial camera are fixedly connected with the lens barrel seat, a z-axis stepping motor is further arranged on the z-axis seat, a screw hole is formed in the lens barrel seat, the z-axis stepping motor is fixedly connected with a z-axis screw rod, the z-axis screw rod is in threaded connection with the screw hole, and the z-axis stepping motor is used for finely adjusting the focus of the lens barrel seat.

Compared with the prior art, the portable full-automatic high-speed microscopic scanner provided by the invention has the following beneficial effects by adopting the technical scheme:

1. the invention adopts an image acquisition scheme based on continuous scanning of a rectangular view field and a scheme of improving the image acquisition brightness by matching with a stroboscopic head, greatly improves the image acquisition efficiency on the premise of ensuring the image acquisition precision, and reduces the image acquisition to 60 to 120 seconds (related to the number of view fields) from more than 20 minutes before.

2. The scheme of the guide seat with the self-guiding and self-driving structure is adopted, the structure of the objective table is greatly simplified, and the new walking speed is convenient to increase.

3. By using a continuous walking + stroboscopic scheme, a smaller numerical aperture, i.e., na value, can be used in the microscope tube. Therefore, the acquired image has a deeper visual field, the diffraction limit image precision is improved, and the edge deformation and the chromatic dispersion are smaller.

4. And a stroboscopic mode is adopted, and the working time is short, so that the heat dissipation pressure of the illumination light source is reduced, and the LED light source with higher power is convenient to adopt.

5. The scheme of open loop control matched with error value correction is adopted, and the acquisition speed is further improved on the basis of ensuring the splicing precision. The acquisition speed of the scheme of the invention reaches the international leading level within 60 to 120 seconds. As shown in fig. 7, 1392 fields of view are scanned, the scanning time only takes 71 seconds, and the scanning is completed, and the stitched image and the identification image are also presented synchronously, which greatly improves the diagnosis efficiency.

6. The light compensation sensor that sets up can realize the luminance between each field of vision unified through luminance compensation in the later stage to the technological problem that stroboscopic working method newly produced has been overcome.

7. The invention adopts mature elements in the prior art to carry out optimized design and accurate control, greatly reduces the equipment cost, is more beneficial to the popularization of a micro scanner in basic medical institutions, benefits more users, intercepts malignant tumors such as cervical cancer and the like at the initial stage, and greatly improves the cure rate of the tumors.

Drawings

The invention is further illustrated by the following examples in conjunction with the accompanying drawings:

fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a perspective view of the subject table support of the present invention.

Fig. 3 is a schematic view of the field of view and path of the present invention.

Fig. 4 is a schematic structural view of a strobe head of the present invention.

FIG. 5 is a schematic view of a slide structure of the present invention.

FIG. 6 is a diagram of the synchronization control pulse of the present invention.

FIG. 7 is a diagram illustrating the scanning result of the apparatus of the present invention.

FIG. 8 is a diagram illustrating another scanning result of the apparatus of the present invention.

In the figure: the device comprises a display device 1, an industrial personal computer 2, a controller 3, a memory 4, a z-axis seat 5, a z-axis stepping motor 6, a z-axis screw 7, a z-axis sliding chute 8, a lens barrel seat 9, a microscope tube 10, an optical compensation sensor 11, a panoramic lens 12, a slide frame 13, a y-axis seat 14, an x-axis seat 15, an x-arm 16, a y-axis motor seat 17, a y-axis stepping motor 18, a y-axis screw 19, a y-arm 20, a y-axis stroke sensor 21, an x-axis motor seat 22, an x-axis stroke sensor 23, an x-axis stepping motor 24, an x-axis screw 25, a base 26, a switch element 27, a switch control signal 28, a power supply 29, a coded image 30, a slide 31, a tissue sample 32, an industrial camera 33, a stroboscopic head 34, an LED light source 35, a visual field 100 and a scanning path 200.

Detailed Description

As shown in fig. 1 and 2, a portable full-automatic high-speed microscopic scanner comprises a stepping object stage, a microscopic lens barrel 10 and a stroboscopic head 15;

the microscope column 10 is connected to an industrial camera 33, and the optical elements in the microscope column 10 are prior art. The microscope tube 10 is fixedly connected with a z-axis seat 5 of the stepping objective table;

the stepping object stage is used for carrying a slide 31; the stepping stage is provided with a recess in the top for carrying the slide racks 13, typically two slides 31 per slide rack 13.

The microscope tube 10 is provided with a strobe head 15 coaxially or on one side, the strobe head 15 is used for emitting strobe light according to a set frequency according to the continuous travel stroke of the stepping object stage, and the industrial camera 33 collects images along with the strobe frequency of the strobe head 15. Preferably, as shown in fig. 4, the strobe head 15 has a structure that the LED light source 35 is electrically connected to the output terminal of the switching element 27, the control terminal of the switching element 27 is electrically connected to the controller, and the input terminal of the switching element 27 is electrically connected to the power supply 29; the switching element 27 in this example is a PMOS transistor.

The maximum brightness of the stroboscopic head 15 is 50000lx to 100000 lx, the stepping speed of the stepping objective table is 17 to 20ms/visual field, the numerical aperture of the microscope column 10 is 0.5 to 0.75, and the exposure time of the industrial camera 33 is 10 to 25 microseconds.

In a preferred embodiment, as shown in fig. 1 and 2, the stepping stage has the following structure: the base 26 is fixedly connected with the z-axis seat 5, the top of the base 26 is provided with an x-direction groove, the x-direction seat 15 is arranged in the x-direction groove and slides along the x direction, the base 26 is provided with an x-axis motor seat 22 extending towards one side of the y direction, the x-axis stepping motor 24 is fixedly connected with the x-axis motor seat 22, one side of the x-direction seat 15 corresponding to the extending x arm 16 is provided with a nut, the x arm 16 is fixedly provided with a nut, the x-axis stepping motor 24 is fixedly connected with an x-axis screw 25, and the x-axis screw 25 is in threaded connection with the nut;

a y-direction groove is formed in the top of the x-direction seat 15, the y-direction seat 14 is arranged in the y-direction groove and slides along the y direction, a y-axis motor seat 17 extending towards one side of the x direction is arranged on the x-direction seat 15, the y-axis motor seat 17 is fixedly connected with a y-axis stepping motor 18, an extending y arm 20 is arranged on one side, corresponding to the y-direction seat 14, a nut is fixedly arranged on the y arm 20, the y-axis stepping motor 18 is fixedly connected with a y-axis screw rod 19, and the y-axis screw rod 19 is in threaded connection with the nut;

a groove opened to the outside is provided on the top of the y-direction holder 14, and the slide rack 13 is disposed in the groove.

Preferably, the base 26, the x-direction seat 15 and the y-direction seat 14 are made of aluminum alloy, and are easily machined into complex shapes. Secondly, the self weight is greatly reduced, and the friction force is reduced. Thirdly, the self-lubricating effect after work hardening is achieved. The fixed structure of the guide structure and the driving device is made into a part of the stepping objective table, so that the dimensional precision is greatly improved, the structure is very compact, and the size of the micro scanner is greatly reduced.

The preferred scheme is as in fig. 1, the x-axis stepping motor 24 and the y-axis stepping motor 18 are connected with the controller 3, and the controller 3 adopts open-loop control; with this structure, high work efficiency is obtained. As shown in fig. 3, 7, the apparatus of the present invention has a field of view 100 of 16:9, 1392 fields of view, namely 48 × 29, of array scanning, takes 71 seconds, and after the scanning is finished, the panorama is spliced, so that the efficiency is greatly improved compared with the prior art. As shown in fig. 8, an artificial intelligence image recognition program is integrated in the industrial personal computer 2, and the range of the tissue sample 32 is recognized through the panorama, so that in the subsequent scanning process, the walking path is optimized, unnecessary content-free views are removed in advance, and the scanning speed is further increased.

The controller 3 is connected with the industrial personal computer 2, the industrial personal computer 2 sends a stepping signal to the controller 3, and the industrial personal computer 2 also sends a stroboscopic signal to the stroboscopic head 34 according to the stepping signal and the position corresponding to the visual field 100; the industrial personal computer 2 adopts the same system as the computer to improve the compatibility of control software, splicing software, identification software and other environment software. The industrial personal computer 2 starts the industrial camera 33 to collect images according to the stroboscopic signal and transmits the images to the memory 4. The controller 3 preferably employs a controller of the STM32F series.

In a preferred scheme, as shown in fig. 1, an x-axis stroke sensor 23 is arranged between an x-axis motor base 22 and an x-axis arm 16, and is used for verifying relative displacement between stroboflash between a base 26 and an x-axis base 15, and if an error is detected, the error value is sent and stored to be used as an x-axis correction value in subsequent image splicing;

and a y-axis stroke sensor 21 is arranged between the y-axis motor base 17 and the y-axis arm 20 and used for checking the relative displacement between stroboflash of the x-axis base 15 and the y-axis base 14, and if an error is detected, the error value is sent and stored to be used as a y-axis correction value in the subsequent image splicing process. With this configuration, the image accuracy is ensured by the post-correction while the open loop control is highly efficient. I.e. an optimization of efficiency and accuracy is achieved. The x-axis stroke sensor 23 and the y-axis stroke sensor 21 in this example are preferably laser displacement sensors. Preferably, the x-axis stroke sensor 23 and the y-axis stroke sensor 21 use light sources of different wavelengths to avoid interference with each other.

In a preferred embodiment, as shown in fig. 1, a display device 1 is further provided, and the display device 1 is integrated on a housing of the micro scanner, so that real-time interaction can be realized. As shown in fig. 7 and 8, the display device 1 preferably employs a touch panel. The display device 1 is connected with the industrial personal computer 2, and after the industrial personal computer 2 receives the acquired images, the acquired images are spliced according to the scanning path 200 and the corresponding coordinates of the field of view 100; i.e., the scanned image, is first subjected to target surface cropping. During the calibration process, the coordinates of each field of view are calibrated, namely, only the first field of view and the second field of view in the first row are spliced manually or based on an image recognition algorithm to obtain the relative coordinates between the first field of view and the second field of view, and subsequent images are spliced in a coordinate positioning mode according to the relative coordinates without edge image recognition during the splicing process. During line changing, the relative coordinates of the inter-line view images are obtained through one-time splicing based on manual work or an image recognition algorithm, subsequent line changing is spliced in a coordinate positioning mode, and edge image recognition is not needed in the splicing process.

And introducing an x-direction correction value and a y-direction correction value for correction in the splicing process. Because errors may be generated on the stepping stage due to missing steps, gaps and the like, the accuracy can be ensured by adopting a subsequent correction mode. During calibration, a single pixel under the resolution ratio during splicing corresponds to a real-world size value, and the x-direction correction value and the y-direction correction value can be converted into the number of pixels for compensation and correction, so that the splicing precision of the visual field images is improved.

Preferably, as shown in fig. 1, a light compensation sensor 11 is further provided, the light compensation sensor 11 is configured to detect an intensity value of a strobe light emitted from the strobe head 34 each time the industrial camera 33 acquires an image, and further preferably, a detection time of the light compensation sensor 11 corresponds to an exposure time of the industrial camera 33. The light intensity values for each field of view are averaged and the difference between the intensity value of the strobe light for each field of view and the average is then determined. And when the difference value exceeds a preset range, performing compensation adjustment on the brightness of the current visual field. According to the scheme, the brightness values among the various visual fields are kept average.

A preferred embodiment is shown in fig. 1, in which a panoramic lens 12 is further provided, and the field of view of the panoramic lens 12 covers the entire slide holder 13 or slide 31. As shown in fig. 5, the panoramic lens 12 is used for acquiring a panoramic image of the code image 30 and the tissue sample 32 to acquire information of the current slide, such as the ID code of the current slide 31, and for guiding the subsequent continuous-walking strobe image acquisition operation, such as to assist the stage in performing pre-positioning according to the tissue sample 32.

In use, as shown in fig. 6, the principle of synchronous pulse control is that, in the figure, x is an x-axis step signal, each pulse signal corresponds to one micro-step of the x-axis step motor 24, y is a y-axis step signal, corresponding to one micro-step of the y-axis step motor 18, and the number and width of pulses in fig. 6 are only schematic. The y-axis step signal is used to wrap the scan path 200. L is a flash signal of the strobe head 34, i.e. after receiving a plurality of x-axis stepping signals, the industrial personal computer 2 sends a switch control signal 28 to turn on the switch element 27, and the LED light source 35 of the strobe head 34 emits light. C is an exposure control pulse of the industrial camera 33, and the pulse width of the switching control signal 28 generally corresponds to a time longer than the exposure time corresponding to the exposure control pulse. Lc is a pulse signal of the light compensation sensor 11 starting to collect a signal, and the pulse signal width of Lc is greater than or equal to the exposure control pulse signal width, and the pulse signal width refers to the pulse signal width of high level.

The preferable scheme is as shown in fig. 1, a microscope barrel seat 9 is arranged on a z-axis seat 5, the microscope barrel seat 9 is in sliding connection with the z-axis seat 5 along the z direction, a microscope tube 10 and an industrial camera 33 are fixedly connected with the microscope barrel seat 9, a z-axis stepping motor 6 is further arranged on the z-axis seat 5, a screw hole is formed in the microscope barrel seat 9, the z-axis stepping motor 6 is fixedly connected with a z-axis screw 7, the z-axis screw 7 is in threaded connection with the screw hole, and the z-axis stepping motor 6 is used for finely adjusting the focus of the microscope barrel seat 9. The scheme can focus images among different layers by finely adjusting the focus of the lens barrel seat 9 for pathological tissue images with multilayer structures.

The above-described embodiments are merely preferred technical solutions of the present invention, and should not be construed as limiting the present invention, and the embodiments and features in the embodiments in the present application may be arbitrarily combined with each other without conflict. The scope of the present invention is defined by the claims, and is intended to include equivalents of the features of the claims. I.e., equivalent alterations and modifications within the scope hereof, are also intended to be within the scope of this invention.

Claims (10)

1. A portable full-automatic high-speed microscopic scanner is characterized in that: comprises a stepping object stage, a microscope tube (10) and a stroboscopic head (15);

the microscope tube (10) is connected with an industrial camera (33), and the microscope tube (10) is fixedly connected with a z-axis seat (5) of the stepping objective table;

the stepping object stage is used for carrying a slide (31);

the microscope lens cone (10) is coaxial or one side of the microscope lens cone is also provided with a stroboscopic head (15), the stroboscopic head (15) is used for emitting flashes according to a set frequency according to the continuous walking stroke of the stepping object stage, and the industrial camera (33) collects images along with the flashing frequency of the stroboscopic head (15).

2. The portable full-automatic high-speed microscopic scanner according to claim 1, characterized in that: the structure of the stepping objective table is as follows: the X-axis stepping motor is characterized in that a base (26) is fixedly connected with a z-axis seat (5), an x-direction groove is formed in the top of the base (26), an x-direction seat (15) is arranged in the x-direction groove and slides along the x direction, an x-axis motor seat (22) extending towards one side of the y direction is arranged on the base (26), an x-axis stepping motor (24) is fixedly connected with the x-axis motor seat (22), an extending x-arm (16) is arranged on one side, corresponding to the x-direction seat (15), of the x-axis seat, a nut is fixedly arranged on the x-arm (16), the x-axis stepping motor (24) is fixedly connected with an x-axis screw rod (25), and the x-axis screw rod (25) is in threaded connection with the nut;

a y-direction groove is formed in the top of the x-direction seat (15), the y-direction seat (14) is arranged in the y-direction groove and slides along the y direction, a y-axis motor seat (17) extending towards one side of the x direction is arranged on the x-direction seat (15), the y-axis motor seat (17) is fixedly connected with a y-axis stepping motor (18), an extending y arm (20) is arranged on one side, corresponding to the y-direction seat (14), of the y-direction seat (20), a nut is fixedly arranged on the y arm (20), the y-axis stepping motor (18) is fixedly connected with a y-axis screw rod (19), and the y-axis screw rod (19) is in threaded connection with the nut;

the top of the y-direction seat (14) is provided with a groove body facing to the outside, and the slide rack (13) is arranged in the groove body.

3. The portable full-automatic high-speed microscopic scanner as set forth in claim 2, characterized in that: the x-axis stepping motor (24) and the y-axis stepping motor (18) are connected with the controller (3), and the controller (3) adopts open-loop control;

the controller (3) is connected with the industrial personal computer (2), the industrial personal computer (2) sends a stepping signal to the controller (3), and the industrial personal computer (2) also sends a stroboscopic signal to the stroboscopic head (34) according to the stepping signal and the position corresponding to the visual field (100);

the industrial personal computer (2) starts an industrial camera (33) to collect images according to the stroboscopic signal and transmits the images to the memory (4).

4. A portable full-automatic high-speed microscopic scanner according to claim 3, characterized in that: an x-axis stroke sensor (23) is arranged between the x-axis motor base (22) and the x-axis arm (16) and used for verifying the relative displacement between stroboflash between the base (26) and the x-axis base (15), and if an error is detected, the error value is sent and stored to be used as an x-axis correction value in the subsequent image splicing process;

and a y-axis stroke sensor (21) is arranged between the y-axis motor base (17) and the y-axis arm (20) and is used for verifying the relative displacement between stroboflash between the x-direction base (15) and the y-direction base (14), and if an error is detected, the error value is sent and stored to be used as a y-direction correction value in the subsequent image splicing process.

5. The portable full-automatic high-speed microscopic scanner as set forth in claim 4, characterized in that: the system is also provided with a display device (1), the display device (1) is connected with an industrial personal computer (2), and the industrial personal computer (2) splices the acquired images according to the corresponding coordinates of the field of view (100) according to the scanning path (200) after receiving the acquired images;

and introducing an x-direction correction value and a y-direction correction value for correction in the splicing process.

6. The portable full-automatic high-speed microscopic scanner according to claim 5, characterized in that: the industrial camera (33) is used for acquiring an image, the light compensation sensor (11) is used for detecting the intensity value of the stroboscopic light each time the industrial camera (33) acquires the image, averaging the light intensity values corresponding to all the fields of view, then calculating the difference value between the intensity value of the stroboscopic light and the average value of all the fields of view, and when the difference value exceeds a preset range, performing compensation adjustment on the brightness of the current field of view.

7. The portable full-automatic high-speed microscopic scanner according to claim 1, characterized in that: the stroboscopic head (15) is structurally characterized in that an LED light source (35) is electrically connected with the output end of a switch element (27), the control end of the switch element (27) is electrically connected with a controller, and the input end of the switch element (27) is electrically connected with a power supply (29).

8. The portable full-automatic high-speed microscopic scanner according to claim 1, characterized in that: the maximum brightness of the stroboscopic head (15) is 50000lx to 100000 lx, the stepping speed of the stepping object stage is 17 to 20 ms/visual field, the numerical aperture of the microscope tube (10) is 0.5 to 0.75, and the exposure time of the industrial camera (33) is 10 to 25 microseconds.

9. The portable full-automatic high-speed microscopic scanner according to claim 8, characterized in that: the continuous walking stroboscopic image acquisition system is further provided with a panoramic lens (12), the visual field of the panoramic lens (12) covers the whole slide frame (13) or the slide (31), and the panoramic lens (12) is used for acquiring panoramic images of a coded image (30) and a tissue sample (32) so as to acquire information of the current slide and guide subsequent continuous walking stroboscopic image acquisition operations.

10. The portable full-automatic high-speed microscopic scanner according to claim 1, characterized in that: be equipped with lens barrel seat (9) on z axle bed (5), lens barrel seat (9) and z axle bed (5) are along z to sliding connection, microscope tube (10) and industrial camera (33) and lens barrel seat (9) fixed connection, still be equipped with z axle step motor (6) on z axle bed (5), be equipped with the screw on lens barrel seat (9), z axle step motor (6) and z axle screw (7) fixed connection, z axle screw (7) and screw threaded connection, z axle step motor (6) are used for finely tuning the focus of lens barrel seat (9).

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