CN210775221U - Portable fluorescent cell analysis system - Google Patents
Portable fluorescent cell analysis system Download PDFInfo
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- CN210775221U CN210775221U CN201921641581.1U CN201921641581U CN210775221U CN 210775221 U CN210775221 U CN 210775221U CN 201921641581 U CN201921641581 U CN 201921641581U CN 210775221 U CN210775221 U CN 210775221U
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Abstract
The embodiment of the utility model discloses a portable fluorescent cell analysis system, which comprises a microscopic imaging module, an objective table and a light source; the microscopic imaging module comprises an image acquisition unit and a microscopic amplification unit, and the effective working distance L1 between the image acquisition unit and the objective table is equal to or more than 30mm and equal to or less than L1 and equal to or less than 100mm along the direction vertical to the objective table; the microscopic magnification unit at least comprises a magnification objective lens group, the magnification objective lens group comprises at least one objective lens, and the focal length f of the objective lens is more than or equal to 2mm and less than or equal to 10 mm; the light sources include a bright field light source and at least one fluorescent light source. Adopt above-mentioned technical scheme to guarantee to realize the microscopic formation of image of fluorescence to through rationally setting up effective working distance between image acquisition unit and the objective table and rationally setting up the focus of objective, guarantee the small and exquisite compactness of microscopic formation of image module, increase portable fluorescence cell analysis system's integrated level and portability.
Description
Technical Field
The embodiment of the utility model provides a relate to fluorescence imaging technology field, especially relate to a portable fluorescence cell analysis system.
Background
The microscopic imaging technology is applied more and more at present, is widely applied to the fields of teaching, scientific research, cell analysis and the like, helps people to realize the cognition in the microscopic field, and plays more and more important roles.
Fluorescence microscopy is an important application of microscopy, wherein fluorescence microscopy is to irradiate a sample with an excitation light source with a specific wavelength, the cell sample labeled by fluorescence is excited to generate fluorescence with different wavelengths, and then the morphology and the structure of the cell sample are observed under a fluorescence microscope, so that the absorption, transportation, distribution and positioning of chemical substances and the like of substances in cells are researched.
The traditional biological fluorescence detection needs a professional fluorescence microscope for observation, and although the functions are complete, the equipment is expensive; in addition, in the conventional fluorescence microscope, for example, the conventional CCD or CMOS fluorescence microscope, the chip size of the early CCD or CMOS is relatively small due to the limitation of the technology process and the production cost, but with the development of the technology and the pursuit of the user for the image effect of large pixel and high resolution, the chip size of the existing CCD or CMOS becomes larger and larger, and the cost of the scientific research grade CCD or CMOS camera remains high.
SUMMERY OF THE UTILITY MODEL
In view of this, the embodiment of the present invention provides a portable fluorescent cell analysis system, which solves the technical problems of high cost, large size and poor portability of the portable fluorescent cell analysis system in the prior art.
The embodiment of the utility model provides a portable fluorescent cell analysis system, which comprises a microscopic imaging module, an objective table and a light source;
the microscopic imaging module comprises an image acquisition unit and a microscopic amplification unit; along the direction vertical to the object stage, the effective working distance L1 between the image acquisition unit and the object stage meets the condition that L1 is more than or equal to 30mm and less than or equal to 100 mm; the microscopic amplification unit at least comprises an amplification objective lens group, the amplification objective lens group comprises at least one objective lens, and the focal length f of the objective lens is more than or equal to 2mm and less than or equal to 10 mm;
the light sources include a bright field light source and at least one fluorescent light source.
Optionally, the microscopic imaging module is detachably arranged.
Optionally, the portable fluorescent cell analysis system further comprises a lifting assembly, and the microscopic imaging module is detachably arranged on the lifting assembly.
Optionally, when the portable fluorescent cell analysis system is in a working state, the microscopic imaging module is disposed on a side of the object stage away from the light source;
an opening is formed on the objective table; the microscopic imaging module comprises a first part and a second part, wherein the first part is close to one side of the object stage, the second part is far away from one side of the object stage, and the vertical projection of the first part on the plane of the object stage is positioned in the opening; when the portable fluorescent cell analysis system is in a non-working state, the first part is nested in the opening.
Optionally, the distance between the image acquisition unit and the microscopic magnification unit is adjustable along a direction perpendicular to the stage.
Optionally, the light source is detachably disposed.
Optionally, the portable fluorescent cell analysis system further comprises a lifting assembly, and the light source is detachably arranged on the lifting assembly.
Optionally, when the portable fluorescent cell analysis system is in a working state, the light source is disposed on a side of the stage away from the microscopic imaging module;
an opening is formed on the objective table; the vertical projection of the light source on the plane of the object stage is positioned in the opening; when the portable fluorescent cell analysis system is in a non-working state, the light source is nested in the opening.
Optionally, the bright field light source is located on one side of the object stage away from the micro-imaging module and used for emitting a bright field light beam;
the at least one fluorescent light source comprises a first fluorescent light source used for emitting a first fluorescent light beam, and the emitting direction of the first fluorescent light beam is vertical to the emitting direction of the bright field light beam.
Optionally, the portable fluorescent cell analysis system further comprises a dichroic mirror, an excitation filter, a first emission filter and a first light-gathering module;
the dichroic mirror is positioned between the magnifying objective lens group and the image acquisition unit, is positioned on the propagation paths of the bright field light beams and the first fluorescent light beams, and is used for transmitting the bright field light beams and reflecting the first fluorescent light beams to an observation sample;
the excitation optical filter is positioned between the first fluorescent light source and the dichroic mirror and is used for carrying out narrow-band filtering processing on the first fluorescent light beam;
the first emission optical filter is positioned between the dichroic mirror and the image acquisition unit and is used for filtering fluorescence excited by the observation sample;
the first light-gathering module is positioned between the first fluorescent light source and the excitation filter, and comprises at least one light-gathering lens for gathering the first fluorescent light beam.
Optionally, the bright field light source is located on one side of the object stage away from the micro-imaging module and used for emitting a bright field light beam;
the at least one fluorescent light source comprises at least two second fluorescent light sources, the at least two second fluorescent light sources are located on one side, away from the microscopic imaging module, of the object stage and used for emitting second fluorescent light beams, and an included angle α between the emitting direction of the second fluorescent light beams and the emitting direction of the bright field light beams is 0< α <90 degrees.
Optionally, the portable fluorescent cell analysis system further includes a second emission filter and at least two second light-focusing modules;
the second emission filter is positioned between the objective table and the microscopic imaging module and is used for filtering fluorescence excited by an observation sample;
the second fluorescence module is positioned between the second fluorescence light source and the object stage and corresponds to the second fluorescence light source one by one; each second light condensing module comprises at least one condensing lens for condensing the second fluorescent light beam.
Optionally, the magnifying objective lens group includes a plurality of objective lenses having different magnifications.
Optionally, the microscopic magnification unit further comprises a magnifying eyepiece group, and the magnifying eyepiece group is located on one side of the magnifying objective group away from the objective table;
the magnifying eyepiece group comprises a plurality of eyepieces with different magnifying powers.
Optionally, the magnifying objective group includes a plurality of objectives with different magnifications;
the microscopic amplification unit also comprises an amplification eyepiece group, and the amplification eyepiece group comprises a plurality of eyepieces with different amplification factors;
one said eyepiece and one said objective lens form a combined lens.
Optionally, the portable fluorescent cell analysis system further comprises a control module, wherein the control module is connected with the microscopic amplification unit and is used for adjusting the amplification factor of the microscopic amplification unit according to the size of the observed sample or based on the amplification requirement of a user.
Optionally, the size of the image capturing unit is less than or equal to 1/3 inches.
Optionally, the image capturing unit further comprises at least one magnifying lens.
Optionally, the image acquisition unit is a movable camera or a mobile terminal with a camera.
The embodiment of the utility model provides a portable fluorescence cell analysis system satisfies through setting up effective working distance L1 between image acquisition unit and the objective table that L1 is less than or equal to 30mm and is less than or equal to 100mm, sets up the micro-magnification unit and includes at least one objective, and the focus f of objective satisfies that 2mm is less than or equal to f and is less than or equal to 10mm, under the prerequisite of guaranteeing fluorescence microscopic imaging, the micro-imaging module is small and exquisite compact, increases portable fluorescence cell analysis's integrated level and portability; and the embodiment of the utility model provides a portable fluorescence cell analysis system need not professional fluorescence microscope alright realize fluorescence detection, has further reduced portable fluorescence cell analysis system's acquisition cost.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments made with reference to the following drawings:
FIG. 1 is a schematic diagram of a portable fluorescence cell analysis system according to an embodiment of the present invention;
fig. 2 is a schematic top view of an objective lens assembly according to an embodiment of the present invention;
fig. 3 is a schematic view of fluorescence microscopy imaging provided by an embodiment of the present invention;
FIG. 4 is a front view of a portable fluorescence cell assay provided by an embodiment of the present invention;
FIG. 5 is a front view of another portable fluorescent cell assay provided by embodiments of the present invention;
fig. 6 is a schematic structural diagram of a micro-imaging module according to an embodiment of the present invention;
fig. 7 is a schematic top view of an objective table according to an embodiment of the present invention;
FIG. 8 is a schematic diagram of the overall structure of another portable fluorescent cell analysis system provided by the embodiment of the present invention;
FIG. 9 is a front view of another portable fluorescent cell analysis system in accordance with an embodiment of the present invention;
FIG. 10 is a front view of another portable fluorescent cell analysis system provided by an embodiment of the present invention;
FIG. 11 is a front view of another portable fluorescent cell analysis system provided by an embodiment of the present invention;
FIG. 12 is a front view of another portable fluorescent cell analysis system provided by an embodiment of the present invention;
fig. 13 is a schematic top view of an enlarged eyepiece set according to an embodiment of the present invention;
fig. 14 is a schematic structural diagram of a combined lens provided in an embodiment of the present invention;
fig. 15 is a schematic structural diagram of an image capturing unit according to an embodiment of the present invention;
fig. 16 is a schematic flow chart of a microscopic imaging method of a portable fluorescent cell analysis system according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail through the following embodiments with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some embodiments of the present invention, not all embodiments, and all other embodiments obtained by those skilled in the art without creative efforts based on the embodiments of the present invention all fall into the protection scope of the present invention.
Fig. 1 is a schematic overall structure diagram of a portable fluorescent cell analysis system provided by an embodiment of the present invention, fig. 2 is a schematic top view structure diagram of an enlarged objective group provided by an embodiment of the present invention, fig. 3 is a schematic fluorescent microscopic imaging diagram provided by an embodiment of the present invention, fig. 4 is a front view of a portable fluorescent cell analysis system provided by an embodiment of the present invention, as shown in fig. 1, fig. 2, fig. 3 and fig. 4, the portable fluorescent cell analysis system provided by an embodiment of the present invention includes a microscopic imaging module 10, a stage 20 and a light source 30; the microscopic imaging module 10 comprises an image acquisition unit 11 and a microscopic amplification unit 12; along the direction vertical to the object stage 20, the effective working distance L1 between the image acquisition unit 11 and the object stage 20 satisfies that L1 is more than or equal to 30mm and less than or equal to 100 mm; the microscopic magnification unit 12 at least comprises a magnifying objective lens group 121, the magnifying objective lens group 121 comprises at least one objective lens 1211, and the focal length f of the objective lens 1211 is more than or equal to 2mm and less than or equal to 10 mm; the light source 30 comprises a bright field light source 31 and at least one fluorescent light source 32.
Further, be different from CCD or CMOS structure among the prior art, the embodiment of the utility model provides an among the portable fluorescence cell analytic system, image acquisition unit 11 can be similar the miniature image acquisition module in the cell-phone camera, uses miniature image acquisition module to microscopic field, cooperates with micro-magnification unit 12, can reduce the volume of micro-imaging module group greatly to reduce whole portable fluorescence cell analytic system's volume, and then reduce portable fluorescence cell analytic system's cost.
Furthermore, in the technical solution of the embodiment of the present invention, along the direction perpendicular to the stage 20 (i.e. the X direction shown in the figure), the effective working distance L1 between the image acquisition unit 11 and the stage 20 satisfies that 30mm is not less than L1 is not less than 100mm, the micro-magnification unit 121 includes at least one objective 1211, and the focal length f of the objective 1211 satisfies that 2mm is not less than f is not less than 10mm, and the effective working distance of the image acquisition unit 11 and the focal length range of the objective 1211 can perform clear micro-imaging on the observation sample with the sample size L2 satisfying that 1 μm is not less than L2 is not less than 120 μm in the large size range, as shown in fig. 3. The embodiment of the utility model provides a scheme is favorable to reducing the size of micro-amplification unit 12, guarantees the small and exquisite compactness of micro-imaging module 10 overall structure, increases portable fluorescence cell analysis system's integrated level and portability.
Further, the embodiment of the utility model provides a portable fluorescence cell analysis system includes bright field light source 31 and at least one fluorescence light source 32 simultaneously through setting up light source 30, guarantees the embodiment of the utility model provides a portable fluorescence cell analysis system can realize normal microscopic imaging and fluorescence microscopic imaging. Aiming at the fluorescence microscopic imaging, a professional fluorescence microscope is not needed, and the cost of the portable fluorescence cell analysis system is reduced.
Optionally, with continued reference to fig. 1, the portable fluorescence cell analysis provided by the embodiment of the present invention further includes an object stage 20, where the object stage 20 is used for carrying an observation sample. Optionally, the bright field light source 31 may include at least one of an LED light source, an OLED light source, or a laser light source, and the fluorescent light source 32 may include at least one of an LED light source, an OLED light source, or a laser light source, and embodiments of the present invention are not limited to the specific form of the bright field light source 31 and the fluorescent light source 32.
It should be noted that the position of the fluorescent light source 32 provided in the embodiment of the present invention is not limited, but different fluorescent light paths will be described in detail in the following embodiments, and fig. 1 illustrates only the position of the fluorescent light source 32 corresponding to one possible fluorescent light path.
To sum up, the embodiment of the present invention provides a portable fluorescent cell analysis system, which is similar to a micro image acquisition module in a mobile phone camera by setting an image acquisition unit, applies the micro image acquisition module to the microscopic field, and cooperates with a micro amplification unit to distinguish a CCD or CMOS microscope in the prior art, so as to reduce the volume of a micro imaging module, thereby reducing the volume of the whole portable fluorescent cell analysis system, and further reducing the cost of the portable fluorescent cell analysis system; (ii) a Furthermore, an effective working distance L1 between the image acquisition unit and the objective table is set to be equal to or more than 30mm and equal to or less than L1 and equal to or less than 100mm, at least one objective lens of the microscopic magnification unit is set, and the focal length f of the objective lens is set to be equal to or more than 2mm and equal to or less than 10 mm; clear fluorescence microscopic imaging can be carried out on the observation sample in a large size range between 1 mu m and 120 mu m within the effective working distance and the focal distance range, so that the integral structure of the microscopic imaging module is small and compact, and the integration level and the portability of portable fluorescence cell analysis are improved; and, the embodiment of the utility model provides a portable fluorescence cell analysis system can realize normal microscopic imaging and fluorescence microscopic imaging simultaneously, need not to adopt dedicated fluorescence microscope to the micro-length line of fluorescence, reduces portable fluorescence cell analysis system's acquisition cost.
Optionally, the size of image capture unit 12 is less than or equal to 1/3 inches.
For example, the chip size of the current CCD or CMOS is becoming larger, so that the cost of the scientific CCD or CMOS camera is high. The embodiment of the utility model provides a portable fluorescence cell analysis system is different from traditional CCD or CMOS camera's difference lies in, has used the camera of less image acquisition unit 12 size, has realized the microscopic imaging in cell analysis field, if the image acquisition unit 12 of 1/3 inches sensitization size that uses lower cost has realized better formation of image effect, has accorded with terminal customer's user demand, also very big reduction terminal customer's purchase and use cost.
On the basis of the above embodiment, the microscopic imaging module 10 is detachably arranged.
Exemplarily, be different from the fixed integrated structure that sets up of micro-imaging module in current CCD or CMOS structure, the embodiment of the utility model provides an among the portable fluorescence cell analytic system, the setting can be dismantled to micro-imaging module 10, it is specific, micro-imaging module 10 can dismantle and set up on support column (not shown in the figure), convenience of customers selects different micro-imaging module 10 according to different observation samples, for example when carrying out the micro-imaging of high magnification and the micro-imaging of lower magnification to observing the sample, can directly change different micro-imaging module based on the performance of dismantling of micro-imaging module, each micro-imaging module only need finely tune alright realize clear formation of image with realizing, guarantee to realize clear formation of image under different magnification demands simply fast.
FIG. 4 is a front view of a portable fluorescence cell analysis system according to an embodiment of the present invention,
fig. 5 is a front view of another portable fluorescent cell analysis system provided by the embodiment of the present invention, and it is shown in fig. 1, fig. 4 and fig. 5, the embodiment of the present invention provides a portable fluorescent cell analysis system further including a lifting assembly 40, the micro-imaging module 10 can be detachably disposed on the lifting assembly 40, the lifting assembly 40 can drive the micro-imaging module 10 to move, so as to realize different operating states of the portable fluorescent cell analysis system.
Specifically, as shown in fig. 4, when the portable fluorescent cell analysis system is in an operating state, the micro-imaging module 10 is disposed on a side L of the stage 20 away from the bright field light source 31.
Illustratively, the portable fluorescent cell analysis system is in an operational state, which is understood to mean that the portable fluorescent cell analysis system is in a normal fluorescence microscopic imaging state. When the portable fluorescent cell analysis system is in a normal operating state, the image acquisition unit 11 normally receives the fluorescent signal and converts the fluorescent signal into an electrical signal. At this time, the lifting assembly 40 drives the microscopic imaging module 10 to lift, and the distance L1 between the image acquisition unit 11 and the object stage 20 is set along the direction perpendicular to the object stage 20 (the X direction in the figure), which is equal to or greater than 30mm and equal to or less than L1 and equal to or less than 100mm, so that the size of the microscopic imaging module 10 is reduced on the premise of satisfying the normal photoelectric conversion of the image acquisition unit 11, the size of the portable fluorescent cell analysis system is further reduced, and the development trend of the miniaturization and portability of the portable fluorescent cell analysis system is met.
Further, fig. 6 is a schematic structural diagram of a microscopic imaging module according to an embodiment of the present invention, and fig. 7 is a schematic structural diagram of a top view of an objective table according to an embodiment of the present invention, as shown in fig. 5, 6 and 7, an opening 21 is formed on the objective table 20; the microscopic imaging module 10 comprises a first part 101 close to one side of the object stage 20 and a second part 102 far from one side of the object stage 20, wherein the vertical projection of the first part 101 on the plane of the object stage 20 is positioned in the opening 21; when the portable fluorescent cell assay is in the inoperative state, the first portion 101 is nested within the opening 21.
Illustratively, as shown in fig. 5 and 6, the microscopic imaging module 10 includes a first portion 101 near a side of the stage 20 and a second portion 102 far from the side of the stage 20, wherein the first portion 101 may be the microscopic amplifying unit 12, and the second portion 102 is the image collecting unit 11; or the first part 101 may be a part of the microscopic magnification unit 12, and the second part 102 includes the image acquisition unit 11 and the part of the microscopic magnification unit 12; or the first part 101 comprises the microscopic magnification unit 12 and the partial image acquisition unit 11 and the second part comprises the partial image acquisition unit 11. The embodiment of the present invention does not limit the composition of the first portion 101 and the second portion 102, and fig. 5 and 6 only use the first portion 101 as the microscopic amplification unit 12, and the second portion 102 as the image acquisition unit 11 for illustration. Further, as shown in fig. 5 and 7, an opening 21 is formed on the stage 20, and the vertical projection of the first portion 101 on the plane of the stage 20 is located in the opening 21, so that when the micro-imaging device is in the non-operating state, the lifting assembly 40 can drive the micro-imaging module 10 to descend toward the side close to the stage 20, so that the first portion 101 of the micro-imaging module 10 is nested in the opening 21, thereby ensuring that the portable fluorescent cell analysis system has a small volume in the non-operating state, and is convenient to store and move; and, set up portable fluorescence cell analysis system when inoperative state, the first part 101 nestification of microscopic imaging module 10 can also protect microscopic imaging module 10 in opening 21, avoids external hard thing to collide with the damage microscopic imaging module 10.
Alternatively, the distance between the image acquisition unit 11 and the microscopic magnification unit 12 may be adjustable in a direction perpendicular to the stage 20.
Exemplarily, except that it sets up microscopic imaging module 10 and can dismantle and set up on lifting unit 40, still realize different magnification through whole change microscopic imaging module 10, guarantee to realize clear formation of image simply fast under different magnification demands, can also set up that the distance between image acquisition unit 11 and the microscopic amplification unit 12 is adjustable, through adjusting the distance between image acquisition unit 11 and the microscopic amplification unit 12, realize the magnification change on the basis that does not change optical resolution, can be applied to under same optical resolution, but need through the observation or the detection scene that image amplification is enlarged. And, be different from traditional microscopical fixed multiple switching, the embodiment of the utility model provides a through setting up the distance adjustable between image acquisition unit 11 and the microscopic magnification unit 12, through adjusting the distance between image acquisition unit 11 and the microscopic magnification unit 12 gradually, can realize the switching of continuous magnification.
In the above embodiment, the portable fluorescent cell analysis system is taken as an example of a cell analysis system with the portable fluorescent cell analysis system in the right position, that is, the portable fluorescent cell analysis system sequentially includes the micro-imaging module 10, the stage 20 and the light source 30 from top to bottom. It can be understood that the portable fluorescent cell analysis system provided by the embodiment of the present invention can also be an inverted cell analysis system, that is, the portable fluorescent cell analysis system sequentially includes the light source 30, the object stage 20 and the micro-imaging module 10 in the order from top to bottom, and the inverted portable fluorescent cell analysis system will be described in detail below.
Alternatively, the light source 30 may be detachably disposed.
For example, the light source 30 is detachably arranged, so that the light source can be replaced simply and quickly according to different light source requirements, for example, when a large-area observation sample is subjected to microscopic imaging, a large-area light source or a large-view-angle light source can be replaced, and the large-area observation sample is ensured to be in a bright field area; when the microscopic imaging is carried out on the observation sample with small area, the light source with small area can be replaced, which is beneficial to energy conservation and environmental protection.
On the basis of the above-mentioned embodiment, fig. 8 is the utility model provides another kind of portable fluorescence cell analysis system's overall structure schematic diagram, as shown in fig. 8, the utility model provides a portable cell analysis system can also include lifting unit 40, and light source 30 can be dismantled and set up on lifting unit 40, and lifting unit 40 can drive the motion of light source 30, can form the bright field region of different areas according to the micro-imaging demand, is favorable to improving the definition of micro-imaging.
Further, fig. 9 is another kind of portable fluorescence cell analysis system's that the embodiment is altogether elevation view, as shown in fig. 9, when portable fluorescence cell analysis system is in operating condition, light source 30 sets up in one side that microscopic imaging module 10 was kept away from to objective table 20, drives light source 30 through lifting unit 40 and moves, guarantees can form the bright field region of different areas according to the microscopic imaging demand, is favorable to improving the definition of microscopic imaging.
Further, fig. 10 is a front view of another portable fluorescence cell analysis system according to an embodiment of the present invention, as shown in fig. 7 and 10, an opening 21 is formed on the stage 20; the vertical projection of the light source 30 on the plane of the stage 20 is located within the opening 21; when the portable fluorescent cell analysis system is in a non-working state, the light source 30 is nested in the opening 21, so that when the portable fluorescent cell analysis system is in the non-working state, the lifting assembly 40 can drive the light source 30 to descend to one side close to the objective table 20, so that the light source 30 is nested in the opening 21, and the portable fluorescent cell analysis system is ensured to be small in size and convenient to store and move when in the non-working state; and, set up portable fluorescence cell analysis system when inoperative state, light source 30 nestification can also protect light source 30 in opening 21, avoids external hard object to collide with damage light source 30.
Alternatively, when the portable fluorescent cell analysis system is an inverted cell analysis system, the distance between the image acquisition unit 11 and the microscopic amplification unit 12 in the direction perpendicular to the stage 20 can also be adjusted.
Illustratively, the distance between the image acquisition unit 11 and the microscopic magnification unit 12 is adjustable, and by adjusting the distance between the image acquisition unit 11 and the microscopic magnification unit 12, the magnification change is realized without changing the optical resolution, and the method can be applied to observation or detection scenes which have the same optical resolution but need to be enlarged through images. And, be different from traditional microscopical fixed multiple switching, the embodiment of the utility model provides a through setting up the distance adjustable between image acquisition unit 11 and the microscopic magnification unit 12, through adjusting the distance between image acquisition unit 11 and the microscopic magnification unit 12 gradually, can realize the switching of continuous magnification.
Further, when the portable cell analysis system is an inverted cell analysis system, the micro imaging module 10 may be a detachable structure. Specifically, micro-imaging module 10 can dismantle and set up on support column (not shown in the figure), convenience of customers observes the sample according to the difference and selects different micro-imaging module 10, for example when carrying out the micro-formation of image of high magnification and the micro-formation of image of lower magnification to observing the sample, can be based on the performance of dismantling of micro-imaging module and directly change different micro-imaging module, each micro-imaging module only need finely tune alright realize clear formation of image with realizing, guarantee to realize clear formation of image simply fast under the different magnification demands.
On the basis of the above-described embodiment, the following describes in detail the case of different fluorescence light paths.
First, the bright field light beam is perpendicular to the emission direction of the fluorescent light beam.
Optionally, as shown in fig. 4, fig. 5, fig. 9 and fig. 10, in the portable fluorescence cell analysis system provided in the embodiment of the present invention, the bright field light source 31 is located on one side of the stage 20 away from the microscopic imaging module 10, and the bright field light source 31 is used for emitting a bright field light beam; the at least one fluorescent light source 32 includes a first fluorescent light source 321, the first fluorescent light source 312 is configured to emit a first fluorescent light beam, and an emitting direction of the first fluorescent light beam is perpendicular to an emitting direction of the bright field light beam; fig. 4 and 5 illustrate a portable fluorescent cell analysis system disposed in a normal position, and fig. 9 and 10 illustrate a portable fluorescent cell analysis system disposed in an inverted position.
Illustratively, the bright field light source 31 is positioned on one side of the object stage 20 away from the microscopic imaging module 10, and the bright field light source 31 illuminates an observation sample wafer to form bright field imaging on the observation sample in the object stage 20; the first fluorescent light source 321 is located on one side of the objective 1211 perpendicular to the central axis, so that the emitting direction of the first fluorescent light beam and the emitting direction of the bright field light beam are perpendicular to each other and do not affect each other.
Further, as shown with continued reference to fig. 4, 5, 9 and 10, in order to realize fluorescence microscopic imaging, the portable fluorescent cell analysis system provided by the embodiment of the present invention may further include a dichroic mirror 51, where the dichroic mirror 51 is located between the magnifying objective lens group 12 and the image collecting unit 11, and is located on the propagation path of the bright field light beam and the first fluorescent light beam, and is configured to transmit the bright field light beam and reflect the first fluorescent light beam to the observation sample.
Illustratively, the dichroic mirror 51 may be understood as a half mirror, and the dichroic mirror 51 is located on both propagation paths of the bright field light beam and the first fluorescent light beam, and is configured to transmit the bright field light beam and reflect the first fluorescent light beam to the observation sample. As shown in fig. 4, 5, 9 and 10, dichroic mirror 51 may be located between magnifying objective lens group 12 and image collecting unit 11, but the embodiment of the present invention does not limit the specific position of dichroic mirror 51, for example, dichroic mirror 51 may be located between magnifying objective lens group 12 and image collecting unit 11 without adding other optical elements; when other optical elements are added, the position of dichroic mirror 51 may be changed according to the position of the optical elements.
Further, with continued reference to fig. 4, 5, 9 and 10, in order to improve the fluorescence microscopic imaging effect, the portable fluorescent cell analysis system provided by the embodiment of the present invention may further include an excitation filter 52 and a first emission filter 53; excitation filter 52 is located between first fluorescent light source 32 and dichroic mirror 51, and is configured to perform narrow-band filtering processing on the first fluorescent light beam; the first emission filter 53 is located between the dichroic mirror 51 and the image collection unit 11, and is configured to perform a filtering process on fluorescence excited by the observation sample.
Exemplarily, as shown in fig. 4, fig. 5, fig. 9 and fig. 10, the embodiment of the present invention provides a portable fluorescent cell analysis system, which can further include an excitation filter 52, wherein the excitation filter 52 is located on the exit path of the first fluorescent light beam, and is used for performing narrow-band filtering processing on the first fluorescent light beam, so as to improve the fluorescent laser efficiency, and simultaneously reduce the interference of the noise fluorescent signal to the fluorescent microscopic imaging, thereby improving the fluorescent microscopic imaging effect. Excitation filter 52 may be located between first fluorescent light source 32 and dichroic mirror 51 without adding other optical elements; when other optical elements are added, the position of excitation filter 52 may be changed according to the position of the optical elements, and the embodiment of the present invention does not limit the position of excitation filter 52, and fig. 4, 5, 9, and 10 only illustrate that excitation filter 52 is located between first fluorescent light source 32 and dichroic mirror 51. The first emission filter 53 is located on a light path of fluorescence excited by the observation sample, and is used for filtering the fluorescence excited by the observation sample, reducing interference of a noise fluorescence signal on fluorescence microscopic imaging, and improving fluorescence microscopic imaging effect. Without adding other optical elements, first emission filter 53 may be located between dichroic mirror 51 and image acquisition unit 11; when other optical elements are added, the position of the first emission filter 53 may be changed according to the position of the optical element, and the embodiment of the present invention does not limit the position of the first emission filter 53, and fig. 4, 5, 9, and 10 only illustrate that the first emission filter 53 is located between the dichroic mirror 51 and the image capturing unit 11.
Further, with continued reference to fig. 4, 5, 9 and 10, in order to improve the converging effect of the first fluorescent light beam 321, the portable fluorescent cell analysis system provided by the embodiments of the present invention may further include a first light-focusing module 54; the first light-gathering module 54 is located between the first fluorescent light source 321 and the excitation filter 52, and includes at least one light-gathering lens for gathering the first fluorescent light beam.
Exemplarily, as shown in fig. 4 and fig. 5, fig. 9 and fig. 10, the portable fluorescence cell analysis system provided by the embodiment of the present invention can further include a first light focusing module 54, and the first light focusing module 54 is located on the exit path of the first fluorescence beam, and is used for focusing the first fluorescence beam, increasing the convergence effect of the first fluorescence beam, and improving the fluorescence microscopic imaging effect. On the premise of not adding other optical elements, the first light-gathering module 54 may be located between the first fluorescent light source 32 and the excitation filter 52; when other optical elements are added, the position of the first light-gathering module 54 can be changed according to the position of the optical elements, the embodiment of the present invention does not limit the position of the first light-gathering module 54, and fig. 4, 5, 9 and 10 only illustrate the case where the first light-gathering module 54 is located between the first fluorescent light source 32 and the excitation filter 52. Optionally, the first light condensing module 54 may include at least one light condensing lens, and fig. 4, fig. 5, fig. 9, and fig. 10 only illustrate that the first light condensing module 54 includes one light condensing lens as an example, embodiments of the present invention do not limit the number of light condensing lenses included in the first light condensing module 54, and one or more light condensing lenses may be disposed according to the convergence requirement.
Next, an example in which an angle between the bright field light beam and the emission direction of the fluorescent light beam is an acute angle will be described.
Fig. 11 is a front view of another portable fluorescent cell analysis system provided by an embodiment of the present invention, fig. 12 is a front view of another portable fluorescent cell analysis system provided by an embodiment of the present invention as shown in fig. 11 and fig. 12, in a portable fluorescent cell analysis system provided by an embodiment of the present invention, a bright field light source 31 is located on one side of the stage 20 away from the microscopic imaging module 10 for emitting a bright field light beam, at least one fluorescent light source 32 includes at least two second fluorescent light sources 322, at least two second fluorescent light sources 322 are both located on one side of the stage 20 away from the microscopic imaging module 10 for emitting a second fluorescent light beam, and an included angle α between an emission direction of the second fluorescent light beam and an emission direction of the bright field light beam satisfies 0< α <90 °.
Further, as shown in fig. 11 and 12, an included angle α between the exit direction of the second fluorescent light beam and the exit direction of the bright field light beam satisfies 0< α <90 °, and the second fluorescent light beam can be directly incident on the observation sample on the stage 20, so as to ensure that the bright field light beam and the second fluorescent light beam do not interfere with each other, on the other hand, a dichroic mirror is not required to be disposed in the technical solution of the embodiment of the present invention, and the volume and the cost of the portable fluorescent cell analysis system can be further reduced.
Optionally, with continued reference to fig. 11 and 12, the portable fluorescence cell analysis system provided by the embodiments of the present invention may further include a second emission filter 55 and at least two second fluorescence modules 56; the second emission filter 55 is located between the object stage 20 and the microscopic imaging module 10, and is used for filtering fluorescence excited by the observation sample; the second light-gathering module 56 is located between the second fluorescent light source 322 and the stage 20, and corresponds to the second fluorescent light source 322 one by one; each second condensing module 56 includes at least one condensing lens for condensing the second fluorescent light beam.
The second emission filter 55 is located on the light path of the fluorescence excited by the observation sample, and is used for filtering the fluorescence excited by the observation sample, reducing the interference of the noise fluorescence signal on the fluorescence microscopic imaging, and improving the fluorescence microscopic imaging effect. The second emission filter 55 may be located between the stage 20 and the micro imaging module 10 without adding other optical elements; when other optical elements are added, the position of the second emission filter 55 can be changed according to the position of the optical elements, the embodiment of the present invention does not limit the position of the second emission filter 55, and fig. 11 and 12 only illustrate the case where the second emission filter 55 is located between the stage 20 and the micro imaging module 10.
In order to improve the converging effect of the second fluorescent light beam 322, the portable fluorescent cell analysis system provided by the embodiment of the present invention may further include a second light-focusing module 56; the second light condensing module 56 is located on the exit path of the second fluorescent light beam, and is configured to condense the second fluorescent light beam, increase the condensing effect of the second fluorescent light beam, and improve the fluorescent microscopic imaging effect. The second light-gathering module 56 can be located between the second fluorescent light source 322 and the stage 20 without adding other optical elements; when other optical elements are added, the position of the second light focusing module 56 can be changed according to the position of the optical elements, the embodiment of the present invention does not limit the position of the second light focusing module 56, and fig. 11 and 12 only illustrate the second light focusing module 56 between the second fluorescent light source 322 and the stage 20. Optionally, the second condensing module 56 may include at least one condensing lens, and fig. 11 and fig. 12 only illustrate that the second condensing module 56 includes one condensing lens, and the embodiment of the present invention does not limit the number of condensing lenses included in the second condensing module 56, and one or more condensing lenses may be disposed according to the condensing requirement.
Further, when the fluorescent microscope comprises at least two second fluorescent light sources 322, two adjacent second fluorescent light sources 322 are arranged around the bright field light source 31 at a fixed interval, so that the second fluorescent light emitted by each second fluorescent light source 322 can be uniformly incident on the observation sample, and the fluorescent microscopic imaging effect is improved.
In summary, the above embodiments illustrate the position relationship between the bright field light source 31 and the fluorescent light source 32 in two different embodiments, and the embodiments of the present invention do not limit the position relationship between the bright field light source 31 and the fluorescent light source 32, but only ensure that the fluorescent microscopic imaging can be realized.
On the basis of the above embodiments, the micro-magnification unit 12 provided in the embodiments of the present invention can have a plurality of different magnifications, and how to implement the plurality of different magnifications will be described in detail below.
With continued reference to fig. 2, the objective lens assembly 121 according to an embodiment of the present invention may include a plurality of objective lenses 1211 with different magnifications.
Exemplarily, as shown in fig. 2, the objective lens assembly 121 for magnifying according to an embodiment of the present invention may include an objective lens turntable 1212 and a plurality of objective lenses 1211 disposed on the objective lens turntable 1212 and having different magnifications, by rotating the objective lens turntable 1212, exchanging the objective lenses 1211 having different magnifications, and selecting the objective lenses 1211 having different magnifications under different magnification requirements, so as to ensure that the microscopic imaging module 10 can be suitable for different magnification requirements, thereby improving the universality of the portable fluorescent cell analysis.
Optionally, fig. 13 is a schematic top view of a magnifying glass group according to an embodiment of the present invention, as shown in fig. 13, the micro-magnifying unit 12 according to an embodiment of the present invention may further include a magnifying glass group 122, the magnifying glass group 122 is located on a side of the magnifying glass group 121 away from the stage 20; the magnifying eyepiece group 122 may include a plurality of eyepieces 1221 different in magnification.
Illustratively, by additionally arranging the magnifying eyepiece group 122 in the microscopic amplification unit 12, and arranging the magnifying eyepiece group 122 at a side of the magnifying objective group 121 far away from the objective table 20, the magnification of the microscopic amplification unit 12 can be further adjusted by the magnifying eyepiece group 122, so as to improve the microscopic imaging effect of the portable fluorescent cell analysis system. Further, as shown in fig. 13, the magnifying glasses group 122 may include an eyepiece dial 1222, and a plurality of eyepieces 1221 with different magnifications are disposed on the eyepiece dial 1222, and the eyepieces 1221 with different magnifications are exchanged by rotating the eyepiece dial 1222. The magnifying eyepiece group 122 may include a plurality of eyepieces 1221 with different magnifications, and for different magnifying requirements, the eyepieces 1221 with different magnifications may be used by adjustment, so as to increase flexibility of magnification adjustment.
It should be noted that fig. 13 only illustrates the magnifying eyepiece set 122 in a feasible manner, and not a limitation on the magnifying eyepiece set 122, and it is understood that other manners of forming the magnifying eyepiece set 122 are also within the scope of the embodiment of the present invention.
Optionally, in the portable fluorescent cell analysis provided in the embodiment of the present invention, the magnifying objective lens group may include a plurality of objective lenses with different magnifying powers; the microscopic magnification unit can also comprise a magnifying eyepiece set, and the magnifying eyepiece set can comprise a plurality of eyepieces with different magnifications; one eyepiece and one objective lens may form a combined lens. Specifically, fig. 14 is a schematic structural diagram of a combined lens provided in the embodiment of the present invention, as shown in fig. 14, an eyepiece 1221 and an objective 1211 form the combined lens 123, and the combined lens 123 is used to perform combined amplification on an observation sample, so as to improve the amplification effect of the observation sample.
To sum up, in the micro-magnification unit provided in the embodiments of the present invention, the magnifying objective lens group may include a plurality of objective lenses with different magnifications; and/or the microscopic magnification unit can also comprise a magnifying eyepiece set, and the magnifying eyepiece set comprises a plurality of eyepieces with different magnifications. Further, eyepiece and objective can also constitute combination camera lens, through the different objective of magnification, and/or, through the different eyepiece of magnification, and/or, through the different combination camera lens of magnification, satisfy the demand of different magnifications, guarantee the embodiment of the utility model provides a portable fluorescence cell analysis possesses good universality.
On the basis of the above-mentioned embodiment, the embodiment of the utility model provides a portable fluorescence cell analysis can also include control module group (not shown in the figure), and the control module group is connected with the micro-magnification unit for adjust according to observing sample size or based on user's enlarged demand the magnification of micro-magnification unit.
Illustratively, according to the size of an observed sample, a user can manually adjust the control module to realize the objective lenses with different magnifications, the ocular lenses with different magnifications and/or the combined lenses with different magnifications to realize the microscopic magnification imaging of the sample to be detected; or the control module receives the amplification requirement of the user, and automatically controls and adjusts the amplification requirement of the user so as to realize the microscopic amplification imaging of the sample to be detected by the objective lens with different amplification factors, the ocular lens with different amplification factors and/or the combined lens with different amplification factors. The embodiment of the utility model provides a accord with the control module group and realize that the magnification switches and not prescribe a limit.
Optionally, fig. 15 is a schematic structural diagram of an image capturing unit according to an embodiment of the present invention, as shown in fig. 15, the image capturing unit 15 may further include at least one magnifying lens 112 in addition to the photoelectric conversion element 111, and fig. 15 only illustrates that the image capturing unit 11 includes one magnifying lens 112.
Illustratively, the magnifying effect of the portable fluorescent cell analysis is further enhanced by adding at least one magnifying lens 112 in the image acquisition unit 11.
Further, the image acquisition unit 11 may be a movable camera, for example, a camera in a mobile terminal; or the image acquisition unit 11 may be a mobile terminal with a camera, such as a mobile phone with a camera. The scheme that the movable camera or the mobile terminal with the camera is combined with at least one objective lens is used for realizing microscopic imaging, so that the portable fluorescent cell analysis structure is simple and the practicability is high.
Based on same utility model design, the embodiment of the utility model provides a still provide a portable fluorescence cell analytic system's microscopic imaging method, this microscopic imaging method is applied to the embodiment of the utility model provides a portable fluorescence cell analytic system. Specifically, fig. 16 is a schematic flow chart of a microscopic imaging method of a portable fluorescent cell analysis system according to an embodiment of the present invention, as shown in fig. 16, the microscopic imaging method according to an embodiment of the present invention includes:
and S110, continuously adjusting the distance between the image acquisition unit and the microscopic amplification unit to a preset distance along the direction vertical to the objective table.
Illustratively, the distance between the image acquisition unit and the microscopic magnification unit can be continuously adjusted. In the microscopic imaging process, the distance between the image acquisition unit and the microscopic amplification unit can be continuously adjusted according to the size of an observed sample or the requirement of a user so as to obtain a clear amplified image of the observed sample or meet the amplification requirement of the user, and at the moment, the distance between the image acquisition unit and the microscopic amplification unit can be understood as a preset distance.
And S120, carrying out microscopic imaging on the observation sample by adopting the portable fluorescent cell analysis system under the preset distance.
Exemplarily, under the above-mentioned predetermined distance, adopt the portable fluorescence cell analysis system that the embodiment of the utility model provides to observe the sample and carry out the micro-imaging, guarantee to obtain clear micro-magnification image.
The embodiment of the utility model provides a microscopic imaging method through the distance between continuous adjustment image acquisition unit and the microscopic magnification unit, can realize the switching of continuous magnification, guarantees to obtain the most clear enlarged image of observation sample or satisfies user's enlarged demand, and microscopic imaging method is simple accurate, and the practicality is high.
It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. Those skilled in the art will appreciate that the present invention is not limited to the specific embodiments described herein, but that the features of the various embodiments of the invention may be partially or fully coupled to each other or combined and may cooperate with each other and be technically driven in various ways. Numerous obvious variations, rearrangements, combinations, and substitutions will now occur to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.
Claims (19)
1. A portable fluorescent cell analysis system is characterized by comprising a microscopic imaging module, an objective table and a light source;
the microscopic imaging module comprises an image acquisition unit and a microscopic amplification unit; along the direction vertical to the object stage, the effective working distance L1 between the image acquisition unit and the object stage meets the condition that L1 is more than or equal to 30mm and less than or equal to 100 mm; the microscopic amplification unit at least comprises an amplification objective lens group, the amplification objective lens group comprises at least one objective lens, and the focal length f of the objective lens is more than or equal to 2mm and less than or equal to 10 mm;
the light sources include a bright field light source and at least one fluorescent light source.
2. The portable fluorescent cell analysis system of claim 1, wherein the microscopic imaging module is removably disposed.
3. The portable fluorescent cell analysis system of claim 2, further comprising a lifting assembly, wherein the microscopic imaging module is detachably disposed on the lifting assembly.
4. The portable fluorescent cell analysis system of claim 3, wherein the microscopic imaging module is disposed on a side of the stage away from the light source when the portable fluorescent cell analysis system is in an operating state;
an opening is formed on the objective table; the microscopic imaging module comprises a first part and a second part, wherein the first part is close to one side of the object stage, the second part is far away from one side of the object stage, and the vertical projection of the first part on the plane of the object stage is positioned in the opening; when the portable fluorescent cell analysis system is in a non-working state, the first part is nested in the opening.
5. The portable fluorescent cell analysis system of claim 1, wherein a distance between the image acquisition unit and the microscopic magnification unit is adjustable in a direction perpendicular to the stage.
6. The portable fluorescent cell analysis system of claim 1, wherein the light source is removably disposed.
7. The portable fluorescent cell analysis system of claim 6, further comprising a lift assembly, the light source being removably disposed on the lift assembly.
8. The portable fluorescent cell analysis system of claim 7, wherein the light source is disposed on a side of the stage away from the micro-imaging module when the portable fluorescent cell analysis system is in an operating state;
an opening is formed on the objective table; the vertical projection of the light source on the plane of the object stage is positioned in the opening; when the portable fluorescent cell analysis system is in a non-working state, the light source is nested in the opening.
9. The portable fluorescent cell analysis system of any one of claims 1 to 8, wherein the bright field light source is located on a side of the stage remote from the microscopic imaging module for emitting a bright field light beam;
the at least one fluorescent light source comprises a first fluorescent light source used for emitting a first fluorescent light beam, and the emitting direction of the first fluorescent light beam is vertical to the emitting direction of the bright field light beam.
10. The portable fluorescent cell analysis system of claim 9, further comprising a dichroic mirror, an excitation filter, a first emission filter, and a first light focusing module;
the dichroic mirror is positioned between the magnifying objective lens group and the image acquisition unit, is positioned on the propagation paths of the bright field light beams and the first fluorescent light beams, and is used for transmitting the bright field light beams and reflecting the first fluorescent light beams to an observation sample;
the excitation optical filter is positioned between the first fluorescent light source and the dichroic mirror and is used for carrying out narrow-band filtering processing on the first fluorescent light beam;
the first emission optical filter is positioned between the dichroic mirror and the image acquisition unit and is used for filtering fluorescence excited by the observation sample;
the first light-gathering module is positioned between the first fluorescent light source and the excitation filter, and comprises at least one light-gathering lens for gathering the first fluorescent light beam.
11. The portable fluorescent cell analysis system of any one of claims 1 to 8, wherein the bright field light source is located on a side of the stage remote from the microscopic imaging module for emitting a bright field light beam;
the at least one fluorescent light source comprises at least two second fluorescent light sources, the at least two second fluorescent light sources are located on one side, away from the microscopic imaging module, of the object stage and used for emitting second fluorescent light beams, and an included angle α between the emitting direction of the second fluorescent light beams and the emitting direction of the bright field light beams is 0< α <90 degrees.
12. The portable fluorescent cell analysis system of claim 11, further comprising a second emission filter and at least two second light focusing modules;
the second emission filter is positioned between the objective table and the microscopic imaging module and is used for filtering fluorescence excited by an observation sample;
the second fluorescence module is positioned between the second fluorescence light source and the object stage and corresponds to the second fluorescence light source one by one; each second light condensing module comprises at least one condensing lens for condensing the second fluorescent light beam.
13. The portable fluorescent cell analysis system of claim 1, wherein the magnifying objective lens set includes a plurality of objective lenses of different magnifications.
14. The portable fluorescent cell analysis system of claim 1, wherein the microscopic magnification unit further comprises a magnifying eyepiece group located on a side of the magnifying eyepiece group remote from the stage;
the magnifying eyepiece group comprises a plurality of eyepieces with different magnifying powers.
15. The portable fluorescent cell analysis system of claim 1, wherein the magnifying objective lens group comprises a plurality of objective lenses of different magnifications;
the microscopic amplification unit also comprises an amplification eyepiece group, and the amplification eyepiece group comprises a plurality of eyepieces with different amplification factors;
one said eyepiece and one said objective lens form a combined lens.
16. The portable fluorescent cell analysis system of any one of claims 13-15, further comprising a control module connected to the micro-magnification unit for adjusting the magnification of the micro-magnification unit according to an observed sample size or based on a user magnification requirement.
17. The portable fluorescent cell analysis system of claim 1, wherein the image acquisition unit has a size less than or equal to 1/3 inches.
18. The portable fluorescent cell analysis system of claim 1, wherein the image acquisition unit further comprises at least one magnifying lens.
19. The portable fluorescent cell analysis system of claim 1, wherein the image acquisition unit is a mobile camera or a mobile terminal with a camera.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112051244A (en) * | 2019-09-29 | 2020-12-08 | 上海睿钰生物科技有限公司 | Portable fluorescent cell analysis system and microscopic imaging method thereof |
| CN112051250A (en) * | 2020-09-09 | 2020-12-08 | 南京诺源医疗器械有限公司 | Medical fluorescence imaging image light supplement adjusting system and adjusting method |
| CN112304851A (en) * | 2020-10-28 | 2021-02-02 | 上海睿钰生物科技有限公司 | Evaluation method of in vitro natural killer cell immunocompetence and application thereof |
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2019
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112051244A (en) * | 2019-09-29 | 2020-12-08 | 上海睿钰生物科技有限公司 | Portable fluorescent cell analysis system and microscopic imaging method thereof |
| CN112051250A (en) * | 2020-09-09 | 2020-12-08 | 南京诺源医疗器械有限公司 | Medical fluorescence imaging image light supplement adjusting system and adjusting method |
| CN112051250B (en) * | 2020-09-09 | 2021-11-23 | 南京诺源医疗器械有限公司 | Medical fluorescence imaging image light supplement adjusting system and adjusting method |
| CN112304851A (en) * | 2020-10-28 | 2021-02-02 | 上海睿钰生物科技有限公司 | Evaluation method of in vitro natural killer cell immunocompetence and application thereof |
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