CN210401827U - Portable cell analysis system - Google Patents
Portable cell analysis system Download PDFInfo
- Publication number
- CN210401827U CN210401827U CN201921641479.1U CN201921641479U CN210401827U CN 210401827 U CN210401827 U CN 210401827U CN 201921641479 U CN201921641479 U CN 201921641479U CN 210401827 U CN210401827 U CN 210401827U
- Authority
- CN
- China
- Prior art keywords
- analysis system
- cell analysis
- microscopic
- portable
- portable cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Microscoopes, Condenser (AREA)
Abstract
The utility model discloses a portable cell analysis system, which comprises a microscopic imaging module, an objective table and a light source; the microscopic imaging module comprises an image acquisition unit and a microscopic amplification unit, and the effective working distance L1 between the image acquisition unit and the objective table is equal to or more than 30mm and equal to or less than L1 and equal to or less than 100mm along the direction vertical to the objective table; the microscopic magnification unit at least comprises a magnification objective lens group, the magnification objective lens group comprises at least one objective lens, and the focal length f of the objective lens is more than or equal to 2mm and less than or equal to 10 mm; the portable cell analysis system is used for carrying out microscopic imaging on an observation sample with the size of L2, wherein L2 is less than or equal to 1 mu m and less than or equal to 120 mu m. By reasonably setting the effective working distance between the image acquisition unit and the objective table and the focal length of the objective lens, the microscopic imaging module is small and compact on the premise of ensuring that the cell analysis system can carry out microscopic imaging on an observation sample in a large-size range between 1 mu m and 120 mu m, the integration level and the portability of the cell analysis system are increased, and the cost of the cell analysis system is reduced.
Description
Technical Field
The embodiment of the utility model provides a relate to microscopic imaging technology field, especially relate to a portable cell analysis system.
Background
Automatic cytometry instruments utilizing microscopic imaging technology are increasingly applied to the fields of teaching, scientific research, cell analysis and the like at present, and help relevant personnel in universities, scientific research institutions and enterprises to meet the requirements of rapid cell counting, cell viability analysis, cell quality management and the like.
The traditional microscopic imaging light path is divided into a finite distance imaging light path and an infinite distance epi-light path; for the limited far optical path, a limited far objective lens is used, and the length of a matched optical cylinder needs to reach 160 mm; for an infinite drop light path, an infinite objective lens is adopted, and because a parallel light path is formed between the objective lens and the tube lens, other optical elements such as a dichroic mirror, an optical filter and the like are added, so that the light path of the whole system is greatly increased, and the size and the volume of the whole system are greatly increased. Moreover, the conventional microscopic imaging technology needs to use image acquisition devices such as CCD and CMOS to present an optical imaging picture at the PC end, and as is known, the chip size of the early CCD or CMOS is relatively small due to the limitations of the technology process and the production cost, but with the development of the technology and the pursuit of users for the image effect of large pixels and high resolution, the chip size of the existing CCD or CMOS becomes larger and larger, and the cost of the scientific research grade CCD or CMOS camera remains high.
SUMMERY OF THE UTILITY MODEL
In view of this, the embodiment of the present invention provides a portable cell analysis system, which solves the technical problems of the prior art, such as large volume, poor portability and high cost.
The embodiment of the utility model provides a portable cell analysis system, which comprises a microscopic imaging module, an objective table and a light source;
the microscopic imaging module comprises an image acquisition unit and a microscopic amplification unit; along the direction vertical to the object stage, the effective working distance L1 between the image acquisition unit and the object stage meets the condition that L1 is more than or equal to 30mm and less than or equal to 100 mm; the microscopic amplification unit at least comprises an amplification objective lens group, the amplification objective lens group comprises at least one objective lens, and the focal length f of the objective lens is more than or equal to 2mm and less than or equal to 10 mm;
the portable cell analysis system is used for carrying out microscopic imaging on an observation sample with the size of L2, wherein L2 satisfies the condition that L2 is less than or equal to 1 mu m and less than or equal to 120 mu m.
Optionally, the microscopic imaging module is detachably arranged.
Optionally, the portable cell analysis system further comprises a lifting assembly, and the microscopic imaging module is detachably arranged on the lifting assembly.
Optionally, when the portable cell analysis system is in a working state, the microscopic imaging module is disposed on a side of the stage away from the light source;
an opening is formed on the objective table; the microscopic imaging module comprises a first part and a second part, wherein the first part is close to one side of the object stage, the second part is far away from one side of the object stage, and the vertical projection of the first part on the plane of the object stage is positioned in the opening; the first portion nests within the opening when the portable cellular analysis system is in an inoperative state.
Optionally, the distance between the image acquisition unit and the microscopic magnification unit is adjustable along a direction perpendicular to the stage.
Optionally, the light source is detachably disposed.
Optionally, the portable cell analysis system further comprises a lifting assembly, and the light source is detachably disposed on the lifting assembly.
Optionally, when the portable cell analysis system is in a working state, the light source is disposed on a side of the stage away from the microscopic imaging module;
an opening is formed on the objective table; the vertical projection of the light source on the plane of the object stage is positioned in the opening; when the portable cell analysis system is in a non-working state, the light source is nested in the opening.
Optionally, the magnifying objective lens group includes a plurality of objective lenses having different magnifications.
Optionally, the microscopic magnification unit further comprises a magnifying eyepiece group, and the magnifying eyepiece group is located on one side of the magnifying objective group away from the objective table;
the magnifying eyepiece group comprises a plurality of eyepieces with different magnifying powers.
Optionally, the magnifying objective group includes a plurality of objectives with different magnifications;
the microscopic amplification unit also comprises an amplification eyepiece group, and the amplification eyepiece group comprises a plurality of eyepieces with different amplification factors;
one said eyepiece and one said objective lens form a combined lens.
Optionally, the portable cell analysis system further comprises a control module, wherein the control module is connected to the micro-magnification unit and is used for adjusting the magnification of the micro-magnification unit according to the size of the observed sample or based on the magnification requirement of the user.
Optionally, the size of the image capturing unit is less than or equal to 1/3 inches.
Optionally, the image capturing unit further comprises at least one magnifying lens.
Optionally, the image acquisition unit is a movable camera or a mobile terminal with a camera.
The embodiment of the utility model provides a portable cell analysis system, satisfy through effective working distance L1 that sets up between image acquisition unit and the objective table that 30mm is less than or equal to L1 and is less than or equal to 100mm, it includes at least one objective to set up the micro-magnification unit, and the focus f of objective satisfies that 2mm is less than or equal to f and is less than or equal to 10mm, under the prerequisite of guaranteeing that portable cell analysis system can carry out micro-imaging to the observation sample of jumbo size within range between 1 mu m-120 mu m, the micro-imaging module is small and exquisite compact, in the magnification scope of confirming, be less than traditional imaging optical path far away in the aspect of imaging system whole optical path, the structure is simplified, increase portable cell analysis system's integrated level and; further, adopt the embodiment of the utility model provides a portable cell analysis system can realize clear microscopic imaging, is different from using CCD or CMOS microscope, can save the cost greatly, has more actual popularization meaning.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments made with reference to the following drawings:
FIG. 1 is a schematic diagram of the overall structure of a portable cell analysis system according to an embodiment of the present invention;
fig. 2 is a schematic top view of an objective lens assembly according to an embodiment of the present invention;
fig. 3 is a schematic view of a microscopic imaging provided by an embodiment of the present invention;
FIG. 4 is a front view of a portable cell analysis system according to an embodiment of the present invention;
FIG. 5 is a front view of another portable cell analysis system provided by an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a detachable micro-imaging module according to an embodiment of the present invention;
fig. 7 is a schematic top view of an objective table according to an embodiment of the present invention;
fig. 8 is a schematic view of another microscopic imaging provided by an embodiment of the present invention;
fig. 9 is a schematic view of another microscopic imaging provided by an embodiment of the present invention;
FIG. 10 is a schematic diagram of the overall structure of another portable cell analysis system provided in the embodiments of the present invention;
FIG. 11 is a front view of another portable cell analysis system in accordance with an embodiment of the present invention;
FIG. 12 is a front view of another portable cell analysis system provided by an embodiment of the present invention;
FIG. 13 is a schematic diagram of a portable cellular analysis system based on a microscopic magnification unit including an objective magnifying lens group;
fig. 14 is a schematic top view of an enlarged eyepiece set according to an embodiment of the present invention;
fig. 15 is a schematic structural diagram of a combined lens provided in an embodiment of the present invention;
FIG. 16 is a schematic view of a portable cellular analysis system based on a micro-magnification unit including a combination lens;
FIG. 17 is a schematic illustration of microscopic imaging based on the portable cellular analysis system provided in FIG. 16;
fig. 18 is a schematic structural diagram of an image capturing unit according to an embodiment of the present invention;
FIG. 19 is a schematic diagram of a portable cellular analysis system including magnifying lenses based on an image capture unit;
FIG. 20 is a schematic illustration of microscopic imaging based on the portable cellular analysis system provided in FIG. 19;
fig. 21 is a schematic flow chart of a microscopic imaging method of a portable cell analysis system according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail through the following embodiments with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some embodiments of the present invention, not all embodiments, and all other embodiments obtained by those skilled in the art without creative efforts based on the embodiments of the present invention all fall into the protection scope of the present invention.
Fig. 1 is a schematic overall structure diagram of a portable cell analysis system provided by an embodiment of the present invention, fig. 2 is a schematic top view structure diagram of an enlarged objective group provided by an embodiment of the present invention, fig. 3 is a schematic microscopic imaging diagram provided by an embodiment of the present invention, fig. 4 is a front view of a portable cell analysis system provided by an embodiment of the present invention, as shown in fig. 1, fig. 2, fig. 3 and fig. 4, the portable cell analysis system provided by an embodiment of the present invention includes a microscopic imaging module 10, a stage 20 and a light source 30; the microscopic imaging module 10 comprises an image acquisition unit 11 and a microscopic amplification unit 12; along the direction vertical to the object stage 20, the effective working distance L1 between the image acquisition unit 11 and the object stage 20 satisfies that L1 is more than or equal to 30mm and less than or equal to 100 mm; the microscopic magnification unit 12 at least comprises a magnifying objective lens group 121, the magnifying objective lens group 121 comprises at least one objective lens 1211, and the focal length f of the objective lens 1211 is greater than or equal to 2mm and less than or equal to f and less than or equal to 10 mm; the portable cell analysis system is used for carrying out microscopic imaging on an observation sample with the size of L2, wherein L2 satisfies the condition that L2 is less than or equal to 1 μm and less than or equal to 120 μm.
Be different from CCD or CMOS structure among the prior art, the embodiment of the utility model provides an among the portable cell analytic system, image acquisition unit 11 can be similar the miniature image acquisition module in the cell-phone camera, uses miniature image acquisition module in the microscopic field, with the cooperation of micro-magnification unit 12, can reduce the volume of micro-imaging module greatly to reduce whole portable cell analytic system's volume, and then reduce portable cell analytic system's cost.
Furthermore, in the embodiment of the present invention, along the direction perpendicular to the stage 20 (i.e. the X direction shown in the figure), the effective working distance L1 between the image capturing unit 11 and the stage 20 satisfies that 30mm is not less than L1 is not less than 100mm, the magnifying objective lens assembly 121 includes at least one objective lens 1211, and the focal length f of the objective lens 1211 satisfies that 2mm is not less than f is not less than 10mm, so that the observation sample in the large size range between 1 μm and 120 μm can be clearly micro-imaged in the effective working distance of the image capturing unit 11 and the focal length range of the objective lens 1211, as shown in fig. 3. The scheme of the embodiment of the utility model is favorable for reducing the size of the microscopic amplification unit 12 and ensuring the compact and small overall structure of the microscopic imaging module 10; meanwhile, the effective working distance between the image acquisition unit 11 and the object stage 20 is reduced, the whole volume of the portable cell analysis system is reduced, and the integration level and the portability of the portable cell analysis system are improved.
Optionally, with continued reference to fig. 1, the portable cell analysis system provided by the embodiment of the present invention further includes a stage 20 and a light source 30, where the stage 20 is used for carrying an observation sample. The light source 30 is a bright field light source for illuminating the observation sample, so that the micro-imaging module 10 can perform micro-imaging on the observation sample conveniently. Optionally, the light source 30 may include at least one of an LED light source, an OLED light source, or a laser light source, and the embodiment of the present invention is not limited to the specific form of the light source 30.
To sum up, the embodiment of the present invention provides a portable cell analysis system, which is similar to a micro image acquisition module in a mobile phone camera by setting an image acquisition unit, applies the micro image acquisition module to the microscopic field, and cooperates with a micro amplification unit to distinguish the micro image acquisition module from a CCD or CMOS microscope in the prior art, so as to reduce the volume of a micro imaging module, thereby reducing the volume of the whole portable cell analysis system and further reducing the cost of the portable cell analysis system; furthermore, the effective working distance L1 between the image acquisition unit and the objective table is set to be equal to or more than 30mm and equal to or less than L1 and equal to or less than 100mm, the microscopic magnification unit comprises at least one objective lens, and the focal length f of the objective lens is set to be equal to or more than 2mm and equal to or less than 10 mm; clear microscopic imaging can be carried out on the observation sample in a large size range between 1 mu m and 120 mu m within the effective working distance and the focal distance range, the integral structure of the microscopic imaging module is small and compact, and the integration degree and the portability of the portable cell analysis system are improved.
Optionally, the size of image capture unit 12 is less than or equal to 1/3 inches.
For example, the chip size of the current CCD or CMOS is becoming larger, so that the cost of the scientific CCD or CMOS camera is high. The embodiment of the utility model provides a difference that portable cell analysis system is different from traditional CCD or CMOS camera lies in, has used the camera of less image acquisition unit 12 size, has realized the microscopic imaging in cell analysis field, if the image acquisition unit 12 of 1/3 inches sensitization size that uses lower cost has realized better formation of image effect, has accorded with terminal customer's user demand, also very big reduction terminal customer's purchase and use cost.
On the basis of the above embodiment, the microscopic imaging module 10 is detachably arranged.
Exemplarily, be different from the fixed integrated structure that sets up of micro-imaging module in current CCD or CMOS structure, the embodiment of the utility model provides an among the portable cell analytic system, the setting can be dismantled to micro-imaging module 10, and is concrete, and micro-imaging module 10 can be dismantled and set up on support column (not shown in the figure), convenience of customers selects different micro-imaging module 10 according to different observation samples, for example when carrying out the micro-imaging of high magnification and the micro-imaging of lower magnification to the observation sample, can directly change different micro-imaging module based on the detachability of micro-imaging module, each micro-imaging module only need finely tune alright realize clear formation of image in order to realize, guarantee to realize clear formation of image under different magnification demands simply fast.
Fig. 4 is a front view of a portable cell analysis system provided by an embodiment of the present invention, fig. 5 is a front view of another portable cell analysis system provided by an embodiment of the present invention, which is shown in fig. 1, fig. 4 and fig. 5, the embodiment of the present invention can further include a lifting assembly 40, the micro-imaging module 10 can be detachably disposed on the lifting assembly 40, and the lifting assembly 40 can drive the micro-imaging module 10 to move, so as to realize different operating states of the portable cell analysis system.
Specifically, as shown in fig. 4, when the portable cell analysis system is in an operating state, the micro-imaging module 10 is disposed on a side of the stage 20 away from the light source 30.
Illustratively, the portable cellular analysis system being in an operational state is understood to be the portable cellular analysis system being in a normal microscopic imaging state. When the portable cell analysis system is in a normal operation state, the image pickup unit 11 receives the optical signal normally and converts the optical signal into an electrical signal. At this time, the lifting assembly 40 drives the microscopic imaging module 10 to lift, and the setting is along the direction (as shown in the figure, the X direction) perpendicular to the object stage 20, the distance L1 between the image acquisition unit 11 and the object stage 20 is the effective working distance of the image acquisition unit 11, and L1 satisfies that L1 is greater than or equal to 30mm and less than or equal to 100mm, on the premise of satisfying the normal photoelectric conversion of the image acquisition unit 11, the size of the microscopic imaging module 10 is reduced, the size of the portable cell analysis system is further reduced, and the development trend of the miniaturization and the portability of the portable cell analysis system is met.
Further, fig. 6 is a schematic structural diagram of a microscopic imaging module according to an embodiment of the present invention, and fig. 7 is a schematic structural diagram of a top view of an objective table according to an embodiment of the present invention, as shown in fig. 5, 6 and 7, an opening 21 is formed on the objective table 20; the microscopic imaging module 10 comprises a first part 101 close to one side of the object stage 20 and a second part 102 far from one side of the object stage 20, wherein the vertical projection of the first part 101 on the plane of the object stage 20 is positioned in the opening 21; when the portable cell analysis system is in a non-operational state, the first portion 101 is nested within the opening 21.
Illustratively, as shown in fig. 5 and 6, the microscopic imaging module 10 includes a first portion 101 near a side of the stage 20 and a second portion 102 far from the side of the stage 20, wherein the first portion 101 may be the microscopic amplifying unit 12, and the second portion 102 is the image collecting unit 11; or the first part 101 may be a part of the microscopic magnification unit 12, and the second part 102 includes the image acquisition unit 11 and the part of the microscopic magnification unit 12; or the first part 101 comprises the microscopic magnification unit 12 and the partial image acquisition unit 11 and the second part comprises the partial image acquisition unit 11. The embodiment of the present invention does not limit the composition of the first portion 101 and the second portion 102, and fig. 5 and 6 only use the first portion 101 as the microscopic amplification unit 12, and the second portion 102 as the image acquisition unit 11 for illustration. Further, as shown in fig. 5 and 7, an opening 21 is formed on the stage 20, and the vertical projection of the first portion 101 on the plane of the stage 20 is located in the opening 21, so that when the portable cell analysis system is in the non-operating state, the lifting assembly 40 can drive the micro-imaging module 10 to descend toward the side close to the stage 20, so that the first portion 101 of the micro-imaging module 10 is nested in the opening 21, thereby ensuring that the portable cell analysis system has a small volume in the non-operating state, and is convenient for storage and transportation; and, set up portable cell analysis system when inoperative state, the first part 101 nestification of micro-imaging module 10 can also protect micro-imaging module 10 in opening 21, avoids external hard thing to collide with the micro-imaging module 10 of damage.
Alternatively, the distance between the image acquisition unit 11 and the microscopic magnification unit 12 may be adjustable in a direction perpendicular to the stage 20.
Exemplarily, except that it sets up microscopic imaging module 10 and can dismantle and set up on lifting unit 40, still realize different magnification through whole change microscopic imaging module 10, guarantee to realize clear formation of image simply fast under different magnification demands, can also set up that the distance between image acquisition unit 11 and the microscopic amplification unit 12 is adjustable, through adjusting the distance between image acquisition unit 11 and the microscopic amplification unit 12, realize the magnification change on the basis that does not change optical resolution, can be applied to under same optical resolution, but need through the observation or the detection scene that image amplification is enlarged. And, be different from traditional microscopical fixed multiple switching, the embodiment of the utility model provides a through setting up the distance adjustable between image acquisition unit 11 and the microscopic magnification unit 12, through adjusting the distance between image acquisition unit 11 and the microscopic magnification unit 12 gradually, can realize the switching of continuous magnification.
Specifically, fig. 8 is another microscopic imaging schematic diagram provided by the embodiment of the present invention, fig. 9 is another microscopic imaging schematic diagram provided by the embodiment of the present invention, fig. 8 is a microscopic imaging schematic diagram of 10 times magnification when the focal length of the objective 1211 is 8mm, and the distance between the image acquisition unit 11 and the microscopic amplification unit 12 is 56mm, fig. 9 is a microscopic imaging schematic diagram of 2.5 times magnification when the focal length of the objective 1211 is 8mm, and the distance between the image acquisition unit 11 and the microscopic amplification unit 12 is 30 mm; as shown in fig. 8 and 9, by adjusting the distance between the image capturing unit 11 and the microscopic magnification unit 12, magnification change can be realized without changing optical resolution, and microscopic imaging with different magnifications can be realized.
In the above embodiment, the portable cell analysis system is taken as an example of a cell analysis system with the portable cell analysis system in front, that is, the portable cell analysis system includes the micro-imaging module 10, the stage 20 and the light source 30 in order from top to bottom. It is understood that the portable cell analysis system provided by the embodiment of the present invention may also be an inverted cell analysis system, that is, the portable cell analysis system sequentially includes the light source 30, the object stage 20 and the micro-imaging module 10 in the order from top to bottom, and the inverted portable cell analysis system will be described in detail below.
Alternatively, the light source 30 may be detachably disposed.
For example, the light source 30 is detachably arranged, so that the light source can be replaced simply and quickly according to different light source requirements, for example, when a large-area observation sample is subjected to microscopic imaging, a large-area light source or a large-view-angle light source can be replaced, and the large-area observation sample is ensured to be in a bright field area; when the microscopic imaging is carried out on the observation sample with small area, the light source with small area can be replaced, which is beneficial to energy conservation and environmental protection.
On the basis of the above-mentioned embodiment, fig. 10 is the utility model provides another kind of portable cell analysis system's overall structure schematic diagram, as shown in fig. 10, the utility model provides a portable cell analysis system can also include lifting unit 40, and light source 30 can be dismantled and set up on lifting unit 40, and lifting unit 40 can drive light source 30 motion, can form the bright field region of different areas according to the micro-imaging demand, is favorable to improving the definition of micro-imaging.
Further, fig. 11 is another kind of portable cell analysis system's that the embodiment is common the elevation view of the utility model, as shown in fig. 11, when portable cell analysis system is in operating condition, light source 30 sets up in one side that microscopic imaging module 10 was kept away from to objective table 20, drives light source 30 through lifting unit 40 and moves, guarantees can form the bright field region of different areas according to the microscopic imaging demand, is favorable to improving the definition of microscopic imaging.
Further, fig. 12 is a front view of another portable cell analysis system according to an embodiment of the present invention, as shown in fig. 7 and 12, an opening 21 is formed on the stage 20; the vertical projection of the light source 30 on the plane of the stage 20 is located within the opening 21; when the portable cell analysis system is in a non-working state, the light source 30 is nested in the opening 21, so that when the portable cell analysis system is in the non-working state, the lifting assembly 40 can drive the light source 30 to descend to one side close to the objective table 20, so that the light source 30 is nested in the opening 21, and the portable cell analysis system is ensured to be small in size and convenient to store and move when in the non-working state; and, set up portable cell analysis system when inoperative state, light source 30 nests and to protect light source 30 in opening 21, avoids external hard thing to collide with damage light source 30.
Alternatively, when the portable cell analysis system is an inverted cell analysis system, the distance between the image pickup unit 11 and the microscopic magnification unit 12 in the direction perpendicular to the stage 20 is also adjustable.
Illustratively, the distance between the image acquisition unit 11 and the microscopic magnification unit 12 is adjustable, and by adjusting the distance between the image acquisition unit 11 and the microscopic magnification unit 12, the magnification change is realized without changing the optical resolution, and the method can be applied to observation or detection scenes which have the same optical resolution but need to be enlarged through images. And, be different from traditional microscopical fixed multiple switching, the embodiment of the utility model provides a through setting up the distance adjustable between image acquisition unit 11 and the microscopic magnification unit 12, through adjusting the distance between image acquisition unit 11 and the microscopic magnification unit 12 gradually, can realize the switching of continuous magnification. The schematic diagram of the microscopic imaging obtained by adjusting the distance between the image acquisition unit 11 and the microscopic amplification unit 12 can also refer to fig. 8 and 9, and is not described here again.
Further, when the portable cell analysis system is an inverted cell analysis system, the micro imaging module 10 may be a detachable structure. Specifically, micro-imaging module 10 can dismantle and set up on support column (not shown in the figure), convenience of customers observes the sample according to the difference and selects different micro-imaging module 10, for example when carrying out the micro-formation of image of high magnification and the micro-formation of image of lower magnification to observing the sample, can be based on the performance of dismantling of micro-imaging module and directly change different micro-imaging module, each micro-imaging module only need finely tune alright realize clear formation of image with realizing, guarantee to realize clear formation of image simply fast under the different magnification demands.
On the basis of the above embodiments, the micro-magnification unit 12 provided in the embodiments of the present invention can have a plurality of different magnifications, and how to implement the plurality of different magnifications will be described in detail below.
Alternatively, as shown in fig. 2, the objective lens assembly 121 provided by the embodiment of the present invention may include a plurality of objective lenses 1211 with different magnifications.
Exemplarily, as shown in fig. 2, the objective lens assembly 121 provided by the embodiment of the present invention may include an objective lens turntable 1212 and a plurality of objective lenses 1211 disposed on the objective lens turntable 1212 and having different magnifications, by rotating the objective lens turntable 1212, exchanging the objective lenses 1211 having different magnifications, and selecting the objective lenses 1211 having different magnifications under different magnification requirements, so as to ensure that the microscopic imaging module 10 can be suitable for different magnification requirements, thereby improving the universality of the portable cell analysis system. Specifically, fig. 13 is a schematic structural diagram of a portable cellular analysis system based on a microscopic magnification unit including a magnifying objective lens group, fig. 3 is a schematic structural diagram of microscopic imaging based on 10-fold magnification of the portable cellular analysis system provided in fig. 13, and as shown in fig. 3 and 13, the microscopic imaging module 10 selects an image acquisition unit 11 with an 1/3-inch target surface and an objective lens 1211 for combined microscopic imaging, which has the same principle as that of conventional microscopic imaging, and realizes different optical magnifications by changing the size of a visual field. Specifically, fig. 3 is a schematic view of microscopic imaging with 10 times magnification when the object plane is unchanged, and the distance L1 between the image acquisition unit 11 and the observation sample (not shown in the figure) is 64mm, as shown in fig. 3, the microscopic imaging module 10 only includes the image acquisition unit 11 and an objective 1211, and when the distance L1 between the image acquisition unit 11 and the observation sample (not shown in the figure) is set to be 64mm, the observation sample can be clearly imaged, which further illustrates that the portable cell analysis system provided by the embodiment of the present invention can realize the miniaturization and portability characteristics of the portable cell analysis system on the premise of ensuring the clear imaging of the observation sample.
Optionally, fig. 14 is a schematic top view structure diagram of a magnifying eyepiece set according to an embodiment of the present invention, as shown in fig. 14, the micro-magnification unit 12 according to an embodiment of the present invention may further include a magnifying eyepiece set 122, and the magnifying eyepiece set 122 is located on a side of the magnifying eyepiece set 121 away from the stage 20; the magnifying eyepiece group 122 may include a plurality of eyepieces 1221 different in magnification.
Illustratively, by additionally arranging the magnifying eyepiece group 122 in the microscopic amplification unit 12, and arranging the magnifying eyepiece group 122 at a side of the magnifying objective group 121 far away from the objective table 20, the magnification of the microscopic amplification unit 12 can be further adjusted by the magnifying eyepiece group 122, so as to improve the microscopic imaging effect of the portable cell analysis system. Further, as shown in fig. 14, the magnifying glasses group 122 may include an eyepiece dial 1222, a plurality of eyepieces 1221 with different magnifications are disposed on the eyepiece dial 1222, and the eyepieces 1221 with different magnifications are exchanged by rotating the eyepiece dial 1222. The magnifying eyepiece group 122 may include a plurality of eyepieces 1221 with different magnifications, and for different magnifying requirements, the eyepieces 1221 with different magnifications may be used by adjustment, so as to increase flexibility of magnification adjustment.
It should be noted that fig. 14 only illustrates the magnifying eyepiece set 122 in a feasible manner, and the magnifying eyepiece set 122 is not limited thereto, and it is understood that other manners of forming the magnifying eyepiece set 122 are also within the scope of the embodiment of the present invention.
Optionally, in the portable cell analysis system provided in the embodiment of the present invention, the magnifying objective lens group may include a plurality of objective lenses with different magnifying powers; the microscopic magnification unit can also comprise a magnifying eyepiece set, and the magnifying eyepiece set can comprise a plurality of eyepieces with different magnifications; one eyepiece and one objective lens may form a combined lens. Specifically, fig. 15 is a schematic structural diagram of a combined lens provided in the embodiment of the present invention, as shown in fig. 15, an eyepiece 1221 and an objective 1211 form the combined lens 123, and the combined lens 123 is used to perform combined amplification on an observation sample, so as to improve the amplification effect of the observation sample.
Specifically, fig. 16 is a schematic structural diagram of a portable cellular analysis system based on a microscope unit including a combined lens, fig. 17 is a schematic structural diagram of a microscope image based on the portable cellular analysis system provided in fig. 16, and as shown in fig. 16 and 17, the microscope imaging module 10 selects the image acquisition unit 11 with the 1/3-inch target surface and the combined lens 123 for combined microscope image. Specifically, fig. 17 is unchanged at the object plane, and the distance L1 between image acquisition unit 11 and the observation sample (not shown in the figure) is the microimaging schematic diagram of 5 times magnification when 44mm, as shown in fig. 17, at microimaging module 10 only including image acquisition unit 11 and combined lens 123, set up distance L1 between image acquisition unit 11 and the observation sample (not shown in the figure) simultaneously and when 44mm, can carry out clear imaging to the observation sample, further explain the utility model provides a portable cell analysis system can realize portable cell analysis system miniaturization and portability characteristics under the prerequisite of guaranteeing to observe the clear formation of image of sample.
To sum up, in the micro-magnification unit provided in the embodiments of the present invention, the magnifying objective lens group may include a plurality of objective lenses with different magnifications; and/or the microscopic magnification unit can also comprise a magnifying eyepiece set, and the magnifying eyepiece set comprises a plurality of eyepieces with different magnifications. Further, eyepiece and objective can also constitute combination camera lens, through the different objective of magnification, and/or, through the different eyepiece of magnification, and/or, through the different combination camera lens of magnification, satisfy the demand of different magnifications, guarantee the embodiment of the utility model provides a portable cell analysis system possesses good universality.
On the basis of the above-mentioned embodiment, the embodiment of the utility model provides a portable cell analysis system can also include control module (not shown in the figure), and the control module is connected with the micro-magnification unit for adjust according to observing sample size or based on user's magnifying requirement the magnification of micro-magnification unit.
Illustratively, according to the size of an observed sample, a user can manually adjust the control module to realize the objective lenses with different magnifications, the ocular lenses with different magnifications and/or the combined lenses with different magnifications to realize the microscopic magnification imaging of the sample to be detected; or the control module receives the amplification requirement of the user, and automatically controls and adjusts the amplification requirement of the user so as to realize the microscopic amplification imaging of the sample to be detected by the objective lens with different amplification factors, the ocular lens with different amplification factors and/or the combined lens with different amplification factors. The embodiment of the utility model provides a accord with the control module group and realize that the magnification switches and not prescribe a limit.
Optionally, fig. 18 is a schematic structural diagram of an image capturing unit according to an embodiment of the present invention, as shown in fig. 18, the image capturing unit 11 may further include at least one magnifying lens 112 in addition to the photoelectric conversion element 111, and fig. 18 only illustrates that the image capturing unit 11 includes one magnifying lens 112.
Illustratively, the magnifying effect of the portable cell analysis system is further enhanced by adding at least one magnifying lens 112 to the image capturing unit 11.
Further, the image acquisition unit 11 may be a movable camera, for example, a camera in a mobile terminal; or the image acquisition unit 11 may be a mobile terminal with a camera, such as a mobile phone with a camera. The scheme that the movable camera or the mobile terminal with the camera is combined with at least one objective lens is used for realizing microscopic imaging, so that the portable cell analysis system is simple in structure and high in practicability.
Specifically, fig. 19 is a schematic structural diagram of a portable cell analysis system based on an image acquisition unit including a magnifying lens, fig. 20 is a schematic microscopic imaging diagram based on the portable cell analysis system provided in fig. 19, and as shown in fig. 19 and 20, a microscopic imaging module 10 selects an image acquisition unit 11 with an 1/3-inch target surface and an objective 1211 for combined microscopic imaging, which is the same as the principle of conventional microscopic imaging, and different optical magnifications are realized by changing the size of a field of view. Specifically, fig. 20 is a schematic view of microscopic imaging with 2.5 times magnification when the distance L1 between the photoelectric conversion element 111 and the observation sample (not shown in the figure) in the image acquisition unit 11 is 38mm, as shown in fig. 20, the microscopic imaging module 10 only includes the image acquisition unit 11 and one objective 1211, and meanwhile, under the condition that the image acquisition unit 111 includes the photoelectric conversion element 111 and at least one magnifying lens 112, and when the distance L1 between the photoelectric conversion element 111 and the observation sample (not shown in the figure) is set to be 38mm, clear imaging can be performed on the observation sample, which further illustrates that the portable cell analysis system provided by the embodiment of the present invention can realize miniaturization and portability of the portable cell analysis system on the premise of ensuring clear imaging of the observation sample.
Based on same utility model design, the embodiment of the utility model provides a still provide a portable cell analysis system's microscopic imaging method, this microscopic imaging method is applied to the embodiment of the utility model provides a portable cell analysis system. Specifically, fig. 21 is a schematic flow chart of a microscopic imaging method of a portable cell analysis system according to an embodiment of the present invention, as shown in fig. 21, the microscopic imaging method according to an embodiment of the present invention includes:
and S110, continuously adjusting the distance between the image acquisition unit and the microscopic amplification unit to a preset distance along the direction vertical to the objective table.
Illustratively, the distance between the image acquisition unit and the microscopic magnification unit can be continuously adjusted. In the microscopic imaging process, the distance between the image acquisition unit and the microscopic amplification unit can be continuously adjusted according to the size of an observed sample or the requirement of a user so as to obtain a clear amplified image of the observed sample or meet the amplification requirement of the user, and at the moment, the distance between the image acquisition unit and the microscopic amplification unit can be understood as a preset distance.
And S120, carrying out microscopic imaging on the observation sample by adopting the portable cell analysis system under the preset distance.
Exemplarily, under the above-mentioned predetermined distance, adopt the portable cell analysis system that the embodiment of the utility model provides to observe the sample and carry out the micro-imaging, guarantee to obtain clear micro-magnification image.
The embodiment of the utility model provides a microscopic imaging method through the distance between continuous adjustment image acquisition unit and the microscopic magnification unit, can realize the switching of continuous magnification, guarantees to obtain the most clear enlarged image of observation sample or satisfies user's enlarged demand, and microscopic imaging method is simple accurate, and the practicality is high.
It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. Those skilled in the art will appreciate that the present invention is not limited to the specific embodiments described herein, but that the features of the various embodiments of the invention may be partially or fully coupled to each other or combined and may cooperate with each other and be technically driven in various ways. Numerous obvious variations, rearrangements, combinations, and substitutions will now occur to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.
Claims (15)
1. A portable cell analysis system is characterized by comprising a microscopic imaging module, an objective table and a light source;
the microscopic imaging module comprises an image acquisition unit and a microscopic amplification unit; along the direction vertical to the object stage, the effective working distance L1 between the image acquisition unit and the object stage meets the condition that L1 is more than or equal to 30mm and less than or equal to 100 mm; the microscopic amplification unit at least comprises an amplification objective lens group, the amplification objective lens group comprises at least one objective lens, and the focal length f of the objective lens is more than or equal to 2mm and less than or equal to 10 mm;
the portable cell analysis system is used for carrying out microscopic imaging on an observation sample with the size of L2, wherein L2 satisfies the condition that L2 is less than or equal to 1 mu m and less than or equal to 120 mu m.
2. The portable cellular analysis system of claim 1, wherein the microscopic imaging module is removably disposed.
3. The portable cell analysis system of claim 2, further comprising a lifting assembly, wherein the micro-imaging module is detachably disposed on the lifting assembly.
4. The portable cell analysis system of claim 3, wherein the microscopic imaging module is disposed on a side of the stage away from the light source when the portable cell analysis system is in an operating state;
an opening is formed on the objective table; the microscopic imaging module comprises a first part and a second part, wherein the first part is close to one side of the object stage, the second part is far away from one side of the object stage, and the vertical projection of the first part on the plane of the object stage is positioned in the opening; the first portion nests within the opening when the portable cellular analysis system is in an inoperative state.
5. The portable cell analysis system according to claim 1, wherein a distance between the image acquisition unit and the microscopic magnification unit is adjustable in a direction perpendicular to the stage.
6. The portable cell analysis system of claim 1, wherein the light source is removably disposed.
7. The portable cell analysis system of claim 6, further comprising a lift assembly, wherein the light source is removably disposed on the lift assembly.
8. The portable cell analysis system of claim 7, wherein the light source is disposed on a side of the stage away from the micro-imaging module when the portable cell analysis system is in an operating state;
an opening is formed on the objective table; the vertical projection of the light source on the plane of the object stage is positioned in the opening; when the portable cell analysis system is in a non-working state, the light source is nested in the opening.
9. The portable cellular analysis system of claim 1, wherein the magnifying objective lens set includes a plurality of objective lenses of different magnifications.
10. The portable cellular analysis system according to claim 1, wherein the microscopic magnification unit further comprises a magnifying eyepiece group located on a side of the magnifying eyepiece group remote from the stage;
the magnifying eyepiece group comprises a plurality of eyepieces with different magnifying powers.
11. The portable cellular analysis system according to claim 1, wherein the magnifying objective lens group includes a plurality of objective lenses having different magnifications;
the microscopic amplification unit also comprises an amplification eyepiece group, and the amplification eyepiece group comprises a plurality of eyepieces with different amplification factors;
one said eyepiece and one said objective lens form a combined lens.
12. A portable cellular analysis system according to any of claims 9 to 11, further comprising a control module connected to the micro-magnification unit for adjusting the magnification of the micro-magnification unit according to the observed sample size or based on user magnification requirements.
13. The portable cellular analysis system of claim 1, wherein the image capture unit has a size less than or equal to 1/3 inches.
14. The portable cellular analysis system according to claim 1, wherein the image acquisition unit further comprises at least one magnifying lens.
15. The portable cell analysis system according to claim 1, wherein the image acquisition unit is a movable camera or a mobile terminal with a camera.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921641479.1U CN210401827U (en) | 2019-09-29 | 2019-09-29 | Portable cell analysis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921641479.1U CN210401827U (en) | 2019-09-29 | 2019-09-29 | Portable cell analysis system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210401827U true CN210401827U (en) | 2020-04-24 |
Family
ID=70343116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921641479.1U Active CN210401827U (en) | 2019-09-29 | 2019-09-29 | Portable cell analysis system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210401827U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112051665A (en) * | 2019-09-29 | 2020-12-08 | 上海睿钰生物科技有限公司 | Portable cell analysis system and microscopic imaging method thereof |
| CN112285082A (en) * | 2020-10-28 | 2021-01-29 | 上海睿钰生物科技有限公司 | Method for evaluating cell killing effect based on cell diameter and application thereof |
| CN112764211A (en) * | 2021-02-04 | 2021-05-07 | 上海睿钰生物科技有限公司 | Microscopic imaging device and microscopic imaging method |
-
2019
- 2019-09-29 CN CN201921641479.1U patent/CN210401827U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112051665A (en) * | 2019-09-29 | 2020-12-08 | 上海睿钰生物科技有限公司 | Portable cell analysis system and microscopic imaging method thereof |
| CN112051665B (en) * | 2019-09-29 | 2024-02-13 | 上海睿钰生物科技有限公司 | Portable cell analysis system and microscopic imaging method thereof |
| CN112285082A (en) * | 2020-10-28 | 2021-01-29 | 上海睿钰生物科技有限公司 | Method for evaluating cell killing effect based on cell diameter and application thereof |
| CN112764211A (en) * | 2021-02-04 | 2021-05-07 | 上海睿钰生物科技有限公司 | Microscopic imaging device and microscopic imaging method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN210401827U (en) | Portable cell analysis system | |
| CN102428401B (en) | Objective lens of endoscope | |
| Roy et al. | Smartphone adapters for digital photomicrography | |
| CN112051244A (en) | Portable fluorescent cell analysis system and microscopic imaging method thereof | |
| WO2021000377A1 (en) | Mobile communication device with microimaging function | |
| CN106707493B (en) | Auxiliary device is shot to microscope | |
| CN109116517B (en) | High-resolution linear scanning machine vision lens with high magnification and large target surface | |
| CN203573023U (en) | Intelligent handset digital microscope | |
| US9019620B2 (en) | Zoom lens, image sensing optical device and digital appliance | |
| CN210775221U (en) | Portable fluorescent cell analysis system | |
| CN104133288A (en) | Continuous zooming and automatic focusing microscopic imaging device and method for living tissues | |
| CN110769144A (en) | Imaging device and mobile terminal | |
| CN112051665A (en) | Portable cell analysis system and microscopic imaging method thereof | |
| KR20070033613A (en) | Portable Digital Microscopes for Mobile Phones | |
| CN112051260A (en) | Portable panoramic cell analysis system and panoramic microscopic imaging method thereof | |
| CN102103262A (en) | Video display ocular lens | |
| CN201955548U (en) | Video display eyepiece | |
| CN210775241U (en) | Portable panoramic cell analysis system | |
| CN204883038U (en) | Sample test is with digital microscope of portable panel computer | |
| CN210376866U (en) | Handheld continuous zoom microscope device | |
| CN210155379U (en) | Device capable of changing position of multiple lenses | |
| CN202093234U (en) | Miniature microscope for digital microscopic image collection device | |
| CN209845031U (en) | Mobile communication equipment with microscopic imaging function | |
| CN103297665A (en) | Image acquisition system | |
| CN203323882U (en) | Hand-held zooming thermal imaging system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |