CN112043822A - Vitis davidii plant extract preparation rich in anthocyanin and preparation method thereof - Google Patents

Vitis davidii plant extract preparation rich in anthocyanin and preparation method thereof Download PDF

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CN112043822A
CN112043822A CN202011023646.3A CN202011023646A CN112043822A CN 112043822 A CN112043822 A CN 112043822A CN 202011023646 A CN202011023646 A CN 202011023646A CN 112043822 A CN112043822 A CN 112043822A
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anthocyanin
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徐昌毅
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Fujian Chunqiu Mingzhu Wine Manor Co ltd
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Abstract

The invention discloses an anthocyanin-rich vitis davidii bunge plant extract preparation and a preparation method thereof, wherein the anthocyanin-rich vitis davidii bunge plant extract preparation comprises the following effective active components in parts by weight: 50-65 parts of spiny grape seed powder, 10-20 parts of anthocyanin, 5-10 parts of lycopene, 5-10 parts of vitamin C and 2-6 parts of tea polyphenol. According to the method, the grease and anthocyanin in the vitis davidii residues are separated from each other, and the grease in the residues is fully utilized, so that the sufficient utilization of the vitis davidii residues is realized, the utilization rate of the vitis davidii is improved, the cost is reduced, the enterprise efficiency is improved, and the economic development is promoted.

Description

Vitis davidii plant extract preparation rich in anthocyanin and preparation method thereof
Technical Field
The invention relates to the technical field of plant extraction, and particularly relates to an anthocyanin-rich vitis davidii plant extract preparation and a preparation method thereof.
Background
Anthocyanins, also known as anthocyanins, are water-soluble natural pigments widely present in plants in nature, and belong to flavonoid compounds. Is easy to be absorbed by human body quickly when being taken orally, and can enter into each tissue and organ of human body quickly after being taken orally. The anthocyanin has good bioavailability in human bodies, strong affinity to connective tissues, long stable half life in an acidic environment and lasting effect. Anthocyanins are widely present in the tissues of all dark red, purple or blue vegetables and fruits, such as purple sweet potato, bilberry, black chokeberry, black currant, blueberry, black (red) rice, elderberry, cranberry, indigo honeysuckle, mulberry, black bean, etc. Anthocyanins are powerful antioxidants, which protect the human body from damage by harmful substances called free radicals, improve the circulatory system and improve the smoothness of the skin, known as "oral cosmetics"; helps prevent various diseases related to free radicals, including cancer, heart disease, and enhances elasticity of artery, vein and capillary vessel; relief of pollinosis and other allergies; as a barrier to protect brain cells from amyloid beta protein formation, glutamate toxicity and free radical attack, thereby preventing Alzheimer's disease; can promote the regeneration of rhodopsin in retinal cells, prevent myopia, and improve vision.
Most of the spine grapes are processed into beverage or fruit wine, but the pomace generated in the processing is mostly used as waste, and the pomace contains a large amount of active substances such as anthocyanin and the like; the spiny grape seeds contain rich anthocyanin, but because the spiny grape seeds have small particles, the extraction process is complex when the anthocyanin is separated, and the waste of resources and energy is inevitable.
Disclosure of Invention
Based on the above circumstances, an object of the present invention is to provide an anthocyanin-rich vitis davidii plant extract preparation and a preparation method thereof, which can effectively solve the above problems.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
an anthocyanin-rich vitis davidii plant extract preparation comprises the following effective active components in parts by weight: 50-65 parts of spiny grape seed powder, 10-20 parts of anthocyanin, 2-10 parts of lycopene, 5-10 parts of vitamin C and 2-6 parts of tea polyphenol.
Preferably, the vitis amurensis plant extract preparation comprises the following effective active components in parts by weight: 65 parts of spine grape seed powder, 20 parts of anthocyanin, 2 parts of lycopene, 10 parts of vitamin C and 3 parts of tea polyphenol.
Preferably, the vitis amurensis plant extract preparation comprises the following effective active components in parts by weight: 50 parts of spine grape seed powder, 15 parts of anthocyanin, 10 parts of lycopene, 5 parts of vitamin C and 5 parts of tea polyphenol.
Preferably, the particle size of the lycopene is 20-35 nm.
The invention also provides a preparation method of the vitis amurensis plant extract preparation rich in anthocyanin, which comprises the following steps:
step one, juicing fresh vitis davidii by using a juicer, and collecting vitis davidii residues for later use;
step two, extracting the vitis amurensis slag, and removing grease in the vitis amurensis slag to obtain an extracting solution rich in anthocyanin; purifying the extracting solution rich in anthocyanin to obtain an extract, and drying the extract to obtain anthocyanin powder;
and step three, uniformly mixing the anthocyanin powder prepared in the step two with the Vitis davidii seed powder, the lycopene, the vitamins and the tea polyphenol according to a ratio to prepare the Vitis davidii plant extract preparation rich in anthocyanin.
Preferably, the second step adopts a microwave drying method or a freeze-drying method to dry the extract.
The preparation process of the anthocyanin-rich extracting solution obtained in the second step comprises the following steps:
putting the vitis davidii residues into an extraction kettle, adding a tetrafluoroethane solution until the vitis davidii residues are submerged, starting extraction, wherein the extraction temperature is 30-40 ℃, the extraction pressure is 20-30MPa, the extraction time is 10-30min, then separating in a separation kettle, the separation temperature is 30-45 ℃, the separation pressure is 10-15MPa, the separation time is 30-60min, obtaining oil in the separation kettle, adding the extract liquid from which the oil is removed into a molecular distiller, setting the vacuum degree in the molecular distiller to be 1.0-2.0Pa, the temperature to be 120-150 ℃ and the rotating speed of a rotary brush membrane to be 1200r/min, until the oil is completely collected by a heavy phase collection tank and a light phase collection tank, and obtaining the anthocyanin-rich extract liquid from which the oil is completely removed.
The spine grape seed powder is prepared by the following processes: and (3) mixing the grease collected by the heavy phase collection tank and the light phase collection tank in the step two with swim bladder collagen and maltodextrin, homogenizing under the high pressure of 30-33MPa for 1-3h, and performing spray drying to obtain the spine grape seed powder.
Preferably, the mass ratio of the grease to the swimming bladder collagen and the maltodextrin is (0.8-1): (1-1.2): (0.5-0.8)
Compared with the prior art, the invention has the following beneficial effects:
the preparation of the invention is added with the swimming bladder collagen, has the efficacies of invigorating qi and blood, nourishing yin, moistening dryness, nourishing the face and strengthening the body, can help the human body to rapidly eliminate fatigue and also has the help function on the recovery of the operation wound. By adding vitamin C and tea polyphenol, the preparation of the invention has richer nutrition. Meanwhile, the oil collected in the second step is mixed with the swim bladder collagen and the maltodextrin to prepare the spiny grape seed powder with the microcapsule structure, so that the reduction and even loss of the activity of the anthocyanin and the collagen are avoided, and the health care function of the preparation is improved.
The vitamin C, the tea polyphenol and the lycopene added in the invention can effectively prolong the shelf life of the preparation, and can also remove free radicals in human bodies and prevent peroxidation in the human bodies after the preparation is taken into the human bodies. The added lycopene has particle size of 20-35nm, and can greatly improve the inclusion degree of raw materials and lycopene, and reduce the possibility of oil adhesion and retention in blood vessel. And partial grease contained in the preparation after being eaten is not easy to oxidize and deposit on the vascular wall, the heart coronary artery and other parts of the human body, so that the problem of oxidation of the grease in the human body after being ingested by the human body is avoided, and the preparation is healthy and safe to eat.
According to the method, the grease and anthocyanin in the vitis davidii residues are separated from each other, and the grease in the residues is fully utilized, so that the sufficient utilization of the vitis davidii residues is realized, the utilization rate of the vitis davidii is improved, the cost is reduced, the enterprise efficiency is improved, and the economic development is promoted.
Detailed Description
In order that those skilled in the art will better understand the technical solutions of the present invention, the following description of the preferred embodiments of the present invention is provided in connection with specific examples, which should not be construed as limiting the present patent.
The test methods or test methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials, unless otherwise indicated, are conventionally obtained commercially or prepared by conventional methods.
Example 1:
an anthocyanin-rich vitis davidii plant extract preparation comprises the following effective active components in parts by weight: 65 parts of spine grape seed powder, 20 parts of anthocyanin, 2 parts of lycopene, 10 parts of vitamin C and 3 parts of tea polyphenol.
Wherein the particle size of lycopene is 20-25 nm.
The embodiment also provides a preparation method of the vitis amurensis plant extract preparation rich in anthocyanin, which comprises the following steps:
step one, juicing fresh vitis davidii by using a juicer, and collecting vitis davidii residues for later use;
step two, extracting the vitis amurensis slag, and removing grease in the vitis amurensis slag to obtain an extracting solution rich in anthocyanin; purifying the extracting solution rich in anthocyanin to obtain an extract, and drying the extract to obtain anthocyanin powder;
and step three, uniformly mixing the anthocyanin powder prepared in the step two with the Vitis davidii seed powder, the lycopene, the vitamins and the tea polyphenol according to a ratio to prepare the Vitis davidii plant extract preparation rich in anthocyanin.
Wherein, the second step adopts a microwave drying method or a freeze-drying method to dry the extract.
The preparation process of the anthocyanin-rich extracting solution obtained in the second step comprises the following steps:
putting the vitis davidii residues into an extraction kettle, adding a tetrafluoroethane solution until the vitis davidii residues are submerged, starting extraction, wherein the extraction temperature is 35 ℃, the extraction pressure is 25MPa, the extraction time is 0.5h, then separating in a separation kettle, the separation temperature is 30 ℃, the separation pressure is 15MPa, the separation time is 0.5h, obtaining oil in the separation kettle, adding the extract liquid without the oil into a molecular distiller, setting the vacuum degree in the molecular distiller to be 1.50Pa, the temperature to be 130 ℃, and the rotating speed of a rotary brush membrane to be 1200r/min until the oil is completely collected by a heavy phase collection tank and a light phase collection tank, and obtaining the anthocyanin-rich extract liquid completely removing the oil.
In this example, the spine grape seed powder was prepared by the following process: and (3) mixing the grease collected by the heavy phase collection tank and the light phase collection tank in the step two with swim bladder collagen and maltodextrin, homogenizing under the high pressure of 30-33MPa for 1-3h, and performing spray drying to obtain the spine grape seed powder.
Preferably, the mass ratio of the grease to the swimming bladder collagen and the maltodextrin is 1: 1: 0.5.
example 2:
an anthocyanin-rich vitis davidii plant extract preparation comprises the following effective active components in parts by weight: 50 parts of spine grape seed powder, 15 parts of anthocyanin, 10 parts of lycopene, 5 parts of vitamin C and 5 parts of tea polyphenol.
Wherein the particle size of lycopene is 25-28 nm.
The embodiment also provides a preparation method of the vitis amurensis plant extract preparation rich in anthocyanin, which comprises the following steps:
step one, juicing fresh vitis davidii by using a juicer, and collecting vitis davidii residues for later use;
step two, extracting the vitis amurensis slag, and removing grease in the vitis amurensis slag to obtain an extracting solution rich in anthocyanin; purifying the extracting solution rich in anthocyanin to obtain an extract, and drying the extract to obtain anthocyanin powder;
and step three, uniformly mixing the anthocyanin powder prepared in the step two with the Vitis davidii seed powder, the lycopene, the vitamins and the tea polyphenol according to a ratio to prepare the Vitis davidii plant extract preparation rich in anthocyanin.
Wherein, the second step adopts a microwave drying method or a freeze-drying method to dry the extract.
The preparation process of the anthocyanin-rich extracting solution obtained in the second step comprises the following steps:
putting the vitis davidii residues into an extraction kettle, adding a tetrafluoroethane solution until the vitis davidii residues are submerged, starting extraction, wherein the extraction temperature is 30 ℃, the extraction pressure is 22MPa, the extraction time is 25min, then separating in a separation kettle, the separation temperature is 45 ℃, the separation pressure is 10MPa, the separation time is 40min, obtaining oil in the separation kettle, adding the extract liquid from which the oil is removed into a molecular distiller, setting the vacuum degree in the molecular distiller to be 2.0Pa, the temperature to be 135 ℃, and the rotating speed of a rotary brush membrane to be 1200r/min until the oil is completely collected by a heavy phase collection tank and a light phase collection tank, so as to obtain the anthocyanin-rich extract liquid from which the oil is completely removed.
The spine grape seed powder of the embodiment is prepared by the following process: and mixing the grease collected by the heavy phase collection tank and the light phase collection tank in the step two with the swim bladder collagen and the maltodextrin, homogenizing for 2 hours under the high pressure of 31MPa, and performing spray drying to obtain the spine grape seed powder.
In the embodiment, the mass ratio of the grease to the air bladder collagen and the maltodextrin is 0.8: 1.2: 0.7.
example 3:
an anthocyanin-rich vitis davidii plant extract preparation comprises the following effective active components in parts by weight: 58 parts of spine grape seed powder, 10 parts of anthocyanin, 5 parts of lycopene, 8 parts of vitamin C and 6 parts of tea polyphenol.
Wherein the particle size of lycopene is 33-35 nm.
The embodiment also provides a preparation method of the vitis amurensis plant extract preparation rich in anthocyanin, which comprises the following steps:
step one, juicing fresh vitis davidii by using a juicer, and collecting vitis davidii residues for later use;
step two, extracting the vitis amurensis slag, and removing grease in the vitis amurensis slag to obtain an extracting solution rich in anthocyanin; purifying the extracting solution rich in anthocyanin to obtain an extract, and drying the extract to obtain anthocyanin powder;
and step three, uniformly mixing the anthocyanin powder prepared in the step two with the Vitis davidii seed powder, the lycopene, the vitamins and the tea polyphenol according to a ratio to prepare the Vitis davidii plant extract preparation rich in anthocyanin.
Wherein, the second step adopts a microwave drying method or a freeze-drying method to dry the extract.
The preparation process of the anthocyanin-rich extracting solution obtained in the second step comprises the following steps:
putting the vitis davidii residues into an extraction kettle, adding a tetrafluoroethane solution until the vitis davidii residues are submerged, starting extraction, wherein the extraction temperature is 38 ℃, the extraction pressure is 30MPa, the extraction time is 10-30min, then separating in a separation kettle, the separation temperature is 35 ℃, the separation pressure is 12MPa, the separation time is 50min, obtaining oil in the separation kettle, adding the extract liquid without the oil into a molecular distiller, setting the vacuum degree in the molecular distiller to be 1.2Pa, the temperature to be 150 ℃, and the rotating speed of a rotary brush membrane to be 1200r/min until the oil is completely collected by a heavy phase collection tank and a light phase collection tank, and obtaining the anthocyanin-rich extract liquid completely removing the oil.
The spine grape seed powder of the embodiment is prepared by the following process: and mixing the grease collected by the heavy phase collection tank and the light phase collection tank in the step two with the swim bladder collagen and the maltodextrin, homogenizing for 3 hours under the high pressure of 30MPa, and performing spray drying to obtain the spine grape seed powder.
In the embodiment, the mass ratio of the grease to the air bladder collagen and the maltodextrin is 0.9: 1.1: 0.6.
example 4:
an anthocyanin-rich vitis davidii plant extract preparation comprises the following effective active components in parts by weight: 50-65 parts of spine grape seed powder, 18 parts of anthocyanin, 7 parts of lycopene, 5 parts of vitamin C and 2 parts of tea polyphenol.
Wherein the particle size of lycopene is 30-35 nm.
The embodiment also provides a preparation method of the vitis amurensis plant extract preparation rich in anthocyanin, which comprises the following steps:
step one, juicing fresh vitis davidii by using a juicer, and collecting vitis davidii residues for later use;
step two, extracting the vitis amurensis slag, and removing grease in the vitis amurensis slag to obtain an extracting solution rich in anthocyanin; purifying the extracting solution rich in anthocyanin to obtain an extract, and drying the extract to obtain anthocyanin powder;
and step three, uniformly mixing the anthocyanin powder prepared in the step two with the Vitis davidii seed powder, the lycopene, the vitamins and the tea polyphenol according to a ratio to prepare the Vitis davidii plant extract preparation rich in anthocyanin.
Wherein, the second step adopts a microwave drying method or a freeze-drying method to dry the extract.
The preparation process of the anthocyanin-rich extracting solution obtained in the second step comprises the following steps:
putting the vitis davidii residues into an extraction kettle, adding a tetrafluoroethane solution until the vitis davidii residues are submerged, starting extraction, wherein the extraction temperature is 40 ℃, the extraction pressure is 26MPa, the extraction time is 10min, then separating in a separation kettle, the separation temperature is 37 ℃, the separation pressure is 13MPa, the separation time is 1.0h, obtaining oil in the separation kettle, adding the extract liquid without the oil into a molecular distiller, setting the vacuum degree in the molecular distiller to be 1Pa, the temperature to be 120 ℃, and the rotating speed of a rotary brush membrane to be 1200r/min until the oil is completely collected by a heavy phase collection tank and a light phase collection tank, so as to obtain the anthocyanin-rich extract liquid without the oil.
The spine grape seed powder of the embodiment is prepared by the following process: and mixing the grease collected by the heavy phase collection tank and the light phase collection tank in the step two with the swim bladder collagen and the maltodextrin, homogenizing for 1h under the high pressure of 33MPa, and performing spray drying to obtain the spine grape seed powder.
In the embodiment, the mass ratio of the grease to the air bladder collagen and the maltodextrin is 1: 1.05: 0.7.
the thorn grape seed powder, the anthocyanin, the lycopene, the vitamin C and the tea polyphenol provided by the invention synergistically play roles in reducing blood fat, softening blood vessels and preventing and treating cardiovascular and cerebrovascular diseases from multiple aspects.
The invention screens the proportion of each component through a large number of experiments, is scientific and reasonable, has high automation degree and high preparation efficiency of the whole preparation process, can well reserve the effective components in the raw materials, does not bring any impurity, has no toxic or side effect, and is suitable for the hyperlipemia population caused by high cholesterol or high triglyceride.
The preparations obtained in examples 1 to 4 were made into edible hard capsules, one capsule corresponding to a preparation amount of 30mg of the raw material, and the comparative example was a placebo of the same quality, and the appearance and taste were substantially identical, and the human body was orally administered twice a day, three capsules each time. The blood lipid levels of the subjects are randomly distributed, the blood lipid is tested once before the subjects eat the blood lipid, the blood lipid is tested again after the subjects eat the blood lipid for 2 months, the eating habits of the subjects are the same, 50 cases are respectively adopted in the experimental groups 1 to 4 corresponding to the examples 1 to 4, and 50 cases are adopted in the control group. Changes in CHOL, TG, and HDL-C in serum before and after the preparation was taken are shown in Table 1.
TABLE 1
Figure BDA0002701475840000061
As can be seen from Table 1, the preparation of the present invention can play a role in assisting in reducing blood lipid.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention, and these modifications and variations should also be considered as within the scope of the invention.

Claims (9)

1. The Vitis davidii willd plant extract preparation rich in anthocyanin is characterized by comprising the following effective active components in parts by weight: 50-65 parts of spiny grape seed powder, 10-20 parts of anthocyanin, 2-10 parts of lycopene, 5-10 parts of vitamin C and 2-6 parts of tea polyphenol.
2. The Vitis davidii Diels plant extract preparation rich in anthocyanidin according to claim 1, comprising the following effective active ingredients in parts by weight: 65 parts of spine grape seed powder, 20 parts of anthocyanin, 2 parts of lycopene, 10 parts of vitamin C and 3 parts of tea polyphenol.
3. The Vitis davidii Diels plant extract preparation rich in anthocyanidin according to claim 1, comprising the following effective active ingredients in parts by weight: 50 parts of spine grape seed powder, 15 parts of anthocyanin, 10 parts of lycopene, 5 parts of vitamin C and 5 parts of tea polyphenol.
4. The anthocyanin-rich Vitis davidii plant extract formulation of claim 1, wherein: the particle size of the lycopene is 20-35 nm.
5. A method of preparing the anthocyanin-rich Vitis davidii plant extract preparation of any one of claims 1 to 4, comprising the steps of:
step one, juicing fresh vitis davidii by using a juicer, and collecting vitis davidii residues for later use;
step two, extracting the vitis amurensis slag, and removing grease in the vitis amurensis slag to obtain an extracting solution rich in anthocyanin; purifying the extracting solution rich in anthocyanin to obtain an extract, and drying the extract to obtain anthocyanin powder;
and step three, uniformly mixing the anthocyanin powder prepared in the step two with the Vitis davidii seed powder, the lycopene, the vitamins and the tea polyphenol according to a ratio to prepare the Vitis davidii plant extract preparation rich in anthocyanin.
6. The method of preparing an anthocyanin-rich Vitis davidii plant extract preparation of claim 5, wherein: and in the second step, drying the extract by adopting a microwave drying method or a freeze-drying method.
7. The method of preparing an anthocyanin-rich Vitis davidii plant extract preparation of claim 5, wherein: the preparation process of the anthocyanin-rich extracting solution obtained in the second step comprises the following steps:
putting the vitis davidii residues into an extraction kettle, adding a tetrafluoroethane solution until the vitis davidii residues are submerged, starting extraction, wherein the extraction temperature is 30-40 ℃, the extraction pressure is 20-30MPa, the extraction time is 10-30min, then separating in a separation kettle, the separation temperature is 30-45 ℃, the separation pressure is 10-15MPa, the separation time is 30-60min, obtaining oil in the separation kettle, adding the extract liquid from which the oil is removed into a molecular distiller, setting the vacuum degree in the molecular distiller to be 1.0-2.0Pa, the temperature to be 120-150 ℃ and the rotating speed of a rotary brush membrane to be 1200r/min, until the oil is completely collected by a heavy phase collection tank and a light phase collection tank, and obtaining the anthocyanin-rich extract liquid from which the oil is completely removed.
8. The method of preparing an anthocyanin-rich Vitis davidii plant extract preparation of claim 7, wherein the Vitis davidii seed powder is prepared by the following process: and (3) mixing the grease collected by the heavy phase collection tank and the light phase collection tank in the step two with swim bladder collagen and maltodextrin, homogenizing under the high pressure of 30-33MPa for 1-3h, and performing spray drying to obtain the spine grape seed powder.
9. The method of preparing an anthocyanin-rich Vitis davidii plant extract formulation of claim 8, wherein: the mass ratio of the grease to the swimming bladder collagen and the maltodextrin is (0.8-1): (1-1.2): (0.5-0.8).
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