CN111642597A

CN111642597A - Medlar and olive tea beverage and preparation method thereof

Info

Publication number: CN111642597A
Application number: CN202010667375.9A
Authority: CN
Inventors: 邸多隆; 段文达; 刘建飞
Original assignee: Lanzhou Institute of Chemical Physics LICP of CAS
Current assignee: Lanzhou Institute of Chemical Physics LICP of CAS
Priority date: 2020-07-13
Filing date: 2020-07-13
Publication date: 2020-09-11

Abstract

The invention provides medlar and olive tea delicacies and a preparation method thereof, and relates to the technical field of foods. The medlar olive tea beverage provided by the invention comprises a medlar polyphenol extract, a hydroxytyrosol extract of olive fruits and an aqueous extract of tea leaves, wherein the medlar polyphenol extract and the hydroxytyrosol extract of olive fruits are both derived from industrial wastes generated in the production of olive oil and wolfberry polysaccharide, so that the waste resources are reused with high value, and the hidden trouble of environmental pollution is solved. The invention also provides a preparation method of the medlar-olive tea treasure, and the medlar-olive tea treasure prepared by the preparation method integrates the good health care effects of 3 extracts, is mellow in taste, fine and smooth in taste and convenient to carry and eat.

Description

Medlar and olive tea beverage and preparation method thereof

Technical Field

The invention belongs to the technical field of food, and particularly relates to medlar olive tea delicacies and a preparation method thereof.

Background

Hydroxytyrosol (HT) is a chemical name of 3, 4-dihydroxy phenylethanol, is a natural polyphenol compound, belongs to iridoid hydroxy aromatic compounds, and is a yellow brown oily liquid. Hydroxytyrosol has good antioxidant activity, can effectively scavenge free radicals and oxides, and is considered to be one of the most natural antioxidants for scavenging free radicals. Modern pharmacological studies have shown that: hydroxytyrosol utensilHas effects in resisting atherosclerosis, preventing cancer, inhibiting oxidative damage, lowering blood sugar, regulating blood lipid, preventing cardiovascular diseases, and relieving inflammation. Meanwhile, the hydroxytyrosol can also prevent and treat various pathological changes caused by smoking, and effectively reduce and inhibit the harm of smoking to human bodies. The European Commission authorizes hydroxytyrosol as a new resource food to be used in general foods (except for children under 3 years old, food of lactating women and pregnant women), and stipulates that the maximum usage amount of hydroxytyrosol in fish oil is 215mg/kg, the maximum usage amount in vegetable oil is 215mg/kg, and the maximum usage amount in margarine is 175 mg/kg. Therefore, the hydroxytyrosol extract has very wide application prospect in the industries of medicine, food and cosmetics. The natural hydroxytyrosol is mainly present in olive leaves and fruits, the content of the natural hydroxytyrosol in the olive leaves is very small and is about 0.2-0.7%, the content of the natural hydroxytyrosol in the olive fruits is relatively high, and the natural hydroxytyrosol is mostly present in a combined biological form except a free form. Due to its high polarity, hydroxytyrosol in the fruit is mainly present in the extracted juice, and most of it is discarded together with the juice. While the content of hydroxytyrosol in olive fruit water is about 1.12 g/L. Statistically, about 0.3 to 0.5m is generated per 1 ton of olive fruit pressed³The olive oil fruit juice. At present, most of the fruit wastewater generated after oil extraction in olive oil production enterprises in China is still treated in a fermentation and composting manner, so that huge resource waste and environmental pollution are caused.

Wolfberry fruit is a dried mature fruit of solanaceae plant wolfberry fruit (Lycium barbarum), is a traditional and famous Chinese medicinal material, is one of the first listed medicinal and edible varieties of the national ministry of health, has more than 2000 years of edible history in China, and is also a nourishing food therapy treasure which is reputed at home and abroad. The medlar is sweet in taste and neutral in nature, and has the effects of nourishing liver and kidney, and replenishing vital essence to improve eyesight. The active substances of fructus Lycii mainly include fructus Lycii polysaccharide, fructus Lycii pigment and fructus Lycii polyphenol. The medlar polyphenol is used as an important secondary metabolite in medlar, has various physiological activities and pharmacological actions such as free radical scavenging, antioxidation, anticancer, antibiosis and the like, has obvious effects on treating and preventing diseases such as tumors, cardiovascular diseases and the like, and has important significance on delaying the aging of human bodies. Due to its good physiological function, it has been widely used as a natural health food additive. With the gradually recognized physiological functions of lycium barbarum flavone, the application of lycium barbarum flavone is increasing. However, at present, food and health food using medlar polyphenol as a main raw material are not reported yet. The applicant researches and develops a preparation method of lycium barbarum polysaccharide on the basis of previous work, and the preparation method is authorized, and abandons an extracting solution with the molecular weight of less than 3kDa while efficiently preparing the lycium barbarum polysaccharide, wherein the abandoned extracting solution is rich in lycium barbarum polyphenol, so that resource waste is caused.

Tea is from China, is called three major beverages in the world together with coffee and cocoa, and mainly contains tea polyphenol. Modern pharmacological studies have shown that: the tea polyphenol can extremely remove harmful free radicals, thereby playing a role in resisting cancers and being beneficial to prevention and adjuvant therapy of various cancers such as intestinal cancer, gastric cancer and the like; and can also prevent and treat cardiovascular diseases, reduce blood lipid and blood sugar, and effectively prevent and treat cerebral apoplexy. Meanwhile, the tea polyphenol can relieve gastrointestinal tension, help digestion, prevent caries, strengthen teeth and eliminate halitosis. Tea drinking has become a living habit of most Chinese people, tea has become an indispensable healthy drink and mental drink in social life, and along with popularization of health consumption concept of people, tea is accepted, loved and pursued by more and more people.

However, with the development of society, consumers have new demands for their favorite products, and they expect that their daily products meet the basic requirements of high efficiency, high quality, safety and stability, and can achieve various health-care effects, and be convenient to store, carry and take.

Disclosure of Invention

In view of the above, the invention aims to provide a medlar-olive tea treasure and a preparation method thereof, which are rich in hydroxytyrosol extract, medlar polyphenol extract and tea polyphenol extract, integrate multiple advantages and health care effects, and provide a new choice for current beverage consumers.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides medlar and olive tea treasure, which comprises the following components in percentage by weight: 40-45% of wolfberry polyphenol extract, 5% of hydroxytyrosol extract of olive fruit and 50-55% of water extract of tea.

Preferably, the medlar olive tea treasure comprises the following components in percentage by weight: 45% of medlar polyphenol extract, 5% of hydroxytyrosol extract of olive fruit and 50% of water extract of tea.

Preferably, the mass content of the medlar polyphenol in the medlar polyphenol extract is more than or equal to 10%.

Preferably, the preparation method of the lycium barbarum polyphenol extract comprises the following steps: (1) concentrating the waste extracting solution in the preparation process of the lycium barbarum polysaccharide to 1/2 of the original volume through the 3kDa lycium barbarum extracting solution to obtain a concentrated solution of the lycium barbarum extracting solution;

(2) pumping the concentrated solution of the medlar extract into a MAR system for adsorption, and removing adsorption residual liquid; after washing with water, removing the washing liquid; eluting with 60% ethanol water solution, collecting eluate, concentrating, and drying to obtain fructus Lycii polyphenol extract.

Preferably, the hydroxytyrosol content in the hydroxytyrosol extract of olive fruits is more than or equal to 15% by mass.

Preferably, the method for preparing hydroxytyrosol extract comprises the following steps: 1) sequentially passing waste fruit water generated by olive oil pressing through a three-leg centrifuge and a tubular centrifuge, and concentrating supernate into extract;

2) mixing n-hexane, ethyl acetate, methanol and water according to a volume ratio of 1 (8-10) to (1-2) to (8-10), and standing for layering to obtain an extraction solvent;

3) mixing the extract with the extraction solvent, stirring for 0.5h, standing for layering, and collecting lower phase extract;

4) concentrating the lower phase extraction liquid and drying to obtain the hydroxytyrosol extract;

there is no chronological order between step 1) and step 2).

Preferably, the volume of the extraction solvent in step 3) is 1/4 of the volume of the waste fruit water in step 1).

Preferably, the aqueous extract of tea leaves comprises an aqueous extract of Pu' er tea.

Preferably, the preparation method of the aqueous extract of tea leaves comprises the following steps: a) mixing tea leaves and water according to a mass ratio of 1: 10-12, carrying out high-speed shearing assisted extraction at 5000rpm for 1.5-2 h, and centrifuging the extracting solution by centrifuges at 3000rpm and 16000rpm in sequence to obtain tea leaf water extracting solution;

b) and concentrating and drying the tea water extract to obtain the water extract of the tea.

The invention provides a preparation method of medlar olive tea delicacies, which comprises the following steps: and mixing the medlar polyphenol extract, the hydroxytyrosol extract of olive fruits and the water extract of tea leaves according to the proportion, putting the mixture into a three-dimensional mixer, fully mixing for 0.5h, packaging and sterilizing to obtain the medlar-olive tea extract.

The invention provides a medlar-olive tea beverage, which comprises medlar polyphenol extract, hydroxytyrosol extract of olive fruit and water extract of tea, wherein the medlar polyphenol extract and the hydroxytyrosol extract of olive fruit are both from industrial wastes generated in the production of olive oil and wolfberry polysaccharide, so that the waste resources are reused in a high-value manner, and the hidden trouble of environmental pollution is solved. The medlar and olive tea delicacies prepared by the preparation method of the invention integrate the good health care efficacy of 3 extracts, and have mellow taste, fine and smooth mouthfeel and convenient carrying and eating.

Drawings

FIG. 1 shows the fingerprint of an extract with the molecular weight of less than 3kDa of Lycium barbarum, the chromatographic conditions are as follows: an elit sepharose ODSC18(250mm × 4.6mm, 5 μm) chromatography column; the mobile phase consists of 0.3 percent glacial acetic acid water (A) and acetonitrile (B); gradient elution conditions: 0-60 min, 95% -75% A; detection wavelength: 310 nm; column temperature: 25 ℃; flow rate: 1.0 mL/min; sample introduction amount: 20 μ L.

Detailed Description

The medlar olive tea delicacy preferably comprises the following components in percentage by weight: 45% of medlar polyphenol extract, 5% of hydroxytyrosol extract of olive fruit and 50% of water extract of tea.

The content of the medlar polyphenol in the medlar polyphenol extract is preferably more than or equal to 10 percent and meets the requirement of a fingerprint spectrum in figure 1; the preparation method of the medlar polyphenol extract preferably comprises the following steps: (1) concentrating the waste extracting solution in the preparation process of the lycium barbarum polysaccharide to 1/2 of the original volume through the 3kDa lycium barbarum extracting solution to obtain a concentrated solution of the lycium barbarum extracting solution;

In the present invention, the extraction method of lycium barbarum polysaccharide is preferably shown in chinese patent CN106478836B (a new method for preparing lycium barbarum polysaccharide). In the step (1) of the present invention, preferably, in the extraction process of lycium barbarum polysaccharides, especially, in the step 5) "of the preparation method shown in CN106478836B," the extraction solution II is concentrated by an ultrafiltration membrane system, and the extraction solution retained by the ultrafiltration membrane "is collected, and then the extraction solution below the ultrafiltration membrane is collected, and the lycium barbarum extraction solution with the molecular weight cutoff of 3kDa is permeated and used as the extraction raw material of lycium barbarum polyphenols, and preferably, the extraction raw material is concentrated to 1/2 of the original volume, so as to obtain the lycium barbarum extraction solution.

During the adsorption in the step (2), the flow speed is preferably set to be 5-10 BV/h (column volume, BV for short), and the adsorption residual liquid is discarded; after adsorption, washing with 10-30 BV of water at a flow rate of 5-10 BV/h, and discarding the washing liquid; and (3) eluting by using 5-8 BV of 60% ethanol water solution in percentage by volume as eluent at the flow rate of 5-10 BV/h, and collecting the eluent. The concentration ratio of the concentration in the step (2) of the invention is preferably 1/5 of the volume of the eluent, and the drying preferably comprises freeze drying or spray drying to obtain the medlar polyphenol. The water content of the medlar polyphenol obtained by drying is preferably 5%.

2MAR columns are preferably connected in parallel in the MAR system, and the MAR column diameter-height ratio is preferably 1: 8-1: 10; the MAR used is preferably a mixture of XDA-8, AB-8 and D101, and the mass ratio of XDA-8, AB-8 and D101 is preferably 2:2: 5.

The mass content of the hydroxytyrosol in the hydroxytyrosol extract of the olive fruits is preferably more than or equal to 15 percent; the method for preparing the hydroxytyrosol extract preferably comprises the following steps: 1) sequentially passing waste fruit water generated by olive oil pressing through a three-leg centrifuge and a tubular centrifuge, and concentrating supernate into extract;

there is no chronological order between step 1) and step 2).

Preferably, after HPLC (high performance liquid chromatography) inspection is carried out on the waste fruit water in the step 1), the content of hydroxytyrosol is more than or equal to 1.0g/L, and the waste fruit water can be used as a raw material for extracting a hydroxytyrosol extract. The centrifugal conditions of the three-foot type centrifuge preferably comprise a rotating speed of 3000rpm and a temperature of 20-40 ℃; the centrifugal conditions of the tubular centrifuge preferably include 16000rpm and the temperature is 20-40 ℃. After the two times of centrifugation, the slurry and other impurities in the fruit water can be removed.

The volume of the extraction solvent in step 3) of the present invention is preferably 1/4 which is equal to the volume of the waste fruit water in step 1). The present invention stirs after the mixing, preferably at a speed of 120 rpm. The invention can be layered after standing after stirring, preferably 1.0h, and the lower phase extraction liquid is collected.

The lower phase extraction liquid is concentrated, and the concentrated state is preferably a fluid extract with the density of 1.05 g/mL. The drying according to the present invention preferably comprises freeze drying or spray drying, and the moisture content of the hydroxytyrosol extract obtained after the drying is preferably 5.0%.

The aqueous extract of the tea leaves preferably comprises an aqueous extract of Pu' er tea; the preparation method of the aqueous extract of tea leaves preferably comprises the following steps: a) mixing tea leaves and water according to a mass ratio of 1: 10-12, carrying out high-speed shearing assisted extraction at 5000rpm for 1.5-2 h, and centrifuging the extracting solution by centrifuges at 3000rpm and 16000rpm in sequence to obtain tea leaf water extracting solution;

Preferably, the concentration in step b) of the present invention is performed by freeze drying or spray drying when the volume of the concentrated solution is 2 times of the volume of the tea leaves, so as to obtain the aqueous extract of the tea leaves. The water content of the aqueous extract of tea leaves according to the invention is preferably 5.0%.

The setting parameters for the three-dimensional mixer preferably include a spindle speed of 25rpm for intensive mixing according to the invention. The package of the present invention preferably has a size of 1.0 g/bag. The sterilization method of the invention preferably comprises Co-60 radiation sterilization, and the sterilization time is 0.5 h.

The eating mode of the medlar olive tea treasure provided by the invention is as follows: one bag (1.0g) of the product is taken, and is added with hot water with the temperature of 60-70 ℃ for brewing, thus the product can be drunk after being brewed.

The following examples are provided to illustrate the preparation method of the present invention, but they should not be construed as limiting the scope of the present invention.

Example 1

1. Preparation of hydroxytyrosol extract:

1) waste fruit water generated by qualified olive oil pressing is sequentially processed by a three-foot centrifuge (3000rpm) and a tubular centrifuge (16000rpm) to remove slurry and other impurities in the fruit water. 500mL of fruit juice is taken and placed in a 1000mL round-bottom flask, and the fruit juice is concentrated to be viscous under reduced pressure;

2) 20mL of n-hexane, 160mL of ethyl acetate, 20mL of methanol and 160mL of water are uniformly mixed, 125mL of the mixture is added into the round-bottom flask obtained in the step 1), the mixture is shaken to dissolve viscous olive polyphenol, and then the dissolved olive polyphenol is transferred to a 500mL separating funnel.

3) Concentrating fruit water to almost fluid extract, adding the mixed solution prepared in step 2), stirring for 0.5h, standing and layering. The lower phase extract was collected.

4) Concentrating the lower phase extract obtained in the step 3), and spray drying to obtain hydroxytyrosol extract.

2. Preparing a medlar polyphenol extract:

1) preparing lycium barbarum polysaccharide by using a method of ZL201611049392(CN106478836B, a preparation method of lycium barbarum polysaccharide), concentrating the extracting solution II obtained in the step 5) through an ultrafiltration membrane system, collecting the extracting solution intercepted by the ultrafiltration membrane, and concentrating the lower membrane solution to 1/2 of the original volume through a 3kDa lycium barbarum extracting solution to obtain a concentrated solution of the lycium barbarum extracting solution.

2) Pumping the concentrated solution of the medlar extract obtained in the step 1) into an MAR system (2 MAR columns are connected in parallel in the MAR system, and the ratio of the diameter to the height of the MAR columns is 1: 8-1: 10; adsorbing with MAR XDA-8: AB-8: D101 at mass ratio of 2:2:5 at flow rate of 5BV/h (column volume, BV for short), and discarding the residual adsorption liquid; after adsorption, washing with 15BV of water at a flow rate of 8BV/h, and discarding the washing liquid; eluting with 6BV 60% (v/v) ethanol at flow rate of 5BV/h, collecting eluate, concentrating the eluate under reduced pressure, and spray drying to obtain fructus Lycii polyphenol; the content of fructus Lycii polyphenol in the prepared fructus Lycii polyphenol extract is not less than 10%, and meets the requirement of fingerprint of figure 1 (fructus Lycii molecular weight is less than 3kDa extract fingerprint).

3. The preparation method of the medlar olive tea treasure comprises the following steps:

1) selecting high-quality Pu' er tea, adding purified water according to a material-liquid ratio of 1:12 (mass ratio), carrying out high-speed shearing assisted extraction at 5000rpm for 1.5h, and centrifuging the extract by centrifuges at 3000rpm and 16000rpm in sequence. Obtaining the tea water extract.

2) Concentrating the tea water extract to 2 times of the volume of the fed amount of tea, and spray drying to obtain the tea extract.

3) Weighing 45 parts of medlar polyphenol extract, 5 parts of hydroxytyrosol extract and 50 parts of tea extract. And (3) fully mixing the components in a three-dimensional mixer for 0.5h, packaging and sterilizing to obtain the medlar-olive tea treasure.

Example 2

1. Preparing a medlar polyphenol extract:

1) the lycium barbarum polysaccharide is prepared by a method of ZL201611049392(CN106478836B, a preparation method of lycium barbarum polysaccharide), the extract II obtained in the step 5 is concentrated by an ultrafiltration membrane system, the extract retained by the ultrafiltration membrane is collected, and the solution obtained after membrane removal is concentrated to 1/2 of the original volume through a 3kDa lycium barbarum extract, so that lycium barbarum extract concentrate is obtained.

2) Pumping the concentrated solution of the medlar extract obtained in the step 1) into an MAR system (2 MAR columns are connected in parallel in the MAR system, and the ratio of the diameter to the height of the MAR columns is 1: 10; adsorbing with MAR XDA-8: AB-8: D101 at mass ratio of 2:2:5 at flow rate of 5BV/h (column volume, BV for short), and discarding the residual adsorption liquid; after adsorption, washing with 20BV of water at a flow rate of 5BV/h, and discarding the washing liquid; eluting with 6BV 60% (v/v) ethanol at flow rate of 5BV/h, collecting eluate, concentrating the eluate under reduced pressure, and spray drying to obtain fructus Lycii polyphenol; the content of fructus Lycii polyphenol in the prepared fructus Lycii polyphenol extract is not less than 10%, and meets the requirement of fingerprint of figure 1 (fructus Lycii molecular weight is less than 3kDa extract fingerprint).

1) selecting high-quality Pu' er tea, adding purified water according to a material-liquid ratio of 1:10 (mass ratio), carrying out high-speed shearing assisted extraction at 5000rpm for 1.5h, and centrifuging the extract by centrifuges at 3000rpm and 16000rpm in sequence. Obtaining the tea water extract.

3) Weighing 40 parts of medlar polyphenol extract, 5 parts of hydroxytyrosol extract obtained in example 1 and 55 parts of tea extract. And (3) fully mixing the components in a three-dimensional mixer for 0.5h, packaging and sterilizing to obtain the medlar-olive tea treasure.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The wolfberry olive tea treasure is characterized by comprising the following components in percentage by weight: 40-45% of wolfberry polyphenol extract, 5% of hydroxytyrosol extract of olive fruit and 50-55% of water extract of tea.

2. The medlar olive tea delicacy according to claim 1, which comprises the following components in percentage by weight: 45% of medlar polyphenol extract, 5% of hydroxytyrosol extract of olive fruit and 50% of water extract of tea.

3. The medlar olive tea delicacy according to claim 1 or 2, wherein the medlar polyphenol content in the medlar polyphenol extract is more than or equal to 10% by mass.

4. The medlar olive tea delicacy of claim 3, wherein the preparation method of the medlar polyphenol extract comprises the following steps: (1) concentrating the waste extracting solution in the preparation process of the lycium barbarum polysaccharide to 1/2 of the original volume through the 3kDa lycium barbarum extracting solution to obtain a concentrated solution of the lycium barbarum extracting solution;

5. The medlar olive tea delicacy according to claim 1 or 2, wherein the hydroxytyrosol content in the hydroxytyrosol extract of the olive fruit is more than or equal to 15% by mass.

6. The wolfberry olive tea delicacy according to claim 5, wherein the preparation method of the hydroxytyrosol extract comprises the following steps: 1) sequentially passing waste fruit water generated by olive oil pressing through a three-leg centrifuge and a tubular centrifuge, and concentrating supernate into extract;

there is no chronological order between step 1) and step 2).

7. The medlar olive tea delicacy of claim 6, wherein the volume of the extraction solvent in the step 3) is 1/4 of the volume of the waste fruit water in the step 1).

8. The medlar olive tea delicacy according to claim 1 or 2, wherein the aqueous extract of tea leaves comprises an aqueous extract of Pu' er tea.

9. The medlar olive tea delicacy of claim 8, wherein the preparation method of the aqueous extract of tea comprises the following steps: a) mixing tea leaves and water according to a mass ratio of 1: 10-12, carrying out high-speed shearing assisted extraction at 5000rpm for 1.5-2 h, and centrifuging the extracting solution by centrifuges at 3000rpm and 16000rpm in sequence to obtain tea leaf water extracting solution;

10. The method for preparing medlar olive tea delicacies according to any one of claims 1 to 9, which is characterized by comprising the following steps: and mixing the medlar polyphenol extract, the hydroxytyrosol extract of olive fruits and the water extract of tea leaves according to the proportion, putting the mixture into a three-dimensional mixer, fully mixing for 0.5h, packaging and sterilizing to obtain the medlar-olive tea extract.