CN112022747B - Itching-relieving and odor-removing plant essence and application thereof - Google Patents
Itching-relieving and odor-removing plant essence and application thereof Download PDFInfo
- Publication number
- CN112022747B CN112022747B CN202010963385.7A CN202010963385A CN112022747B CN 112022747 B CN112022747 B CN 112022747B CN 202010963385 A CN202010963385 A CN 202010963385A CN 112022747 B CN112022747 B CN 112022747B
- Authority
- CN
- China
- Prior art keywords
- extract
- plant essence
- ethanol
- oil
- dissolving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/04—Antipruritics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q15/00—Anti-perspirants or body deodorants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Organic Chemistry (AREA)
- Dermatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Reproductive Health (AREA)
- Endocrinology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Emergency Medicine (AREA)
- Zoology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses an itching-relieving and odor-removing plant essence and application thereof. The plant essence contains cnidium fruit oil, perilla leaf oil, chinese gall extract, radix sophorae flavescentis extract, almond extract, peony root and bark extract, phellodendron extract, menthol and borneol. The plant essence disclosed by the invention has excellent itching-relieving and odor-removing effects, is good in product stability, free of layering and turbid precipitation, highly natural in components, high in safety, simple in preparation method and very good in application in the aspect of producing products capable of relieving itching and removing odor.
Description
Technical Field
The invention belongs to the technical field of daily chemicals. More particularly, relates to an itching-relieving and odor-removing plant essence and application thereof.
Background
Female private parts are important organs for metabolic secretion, are relatively complex due to physiological, fertility, sanitary and clean factors and the like, are influenced by internal and external factors, are susceptible to various germs and cause diseases, not only influence the physical and mental health and the life quality of the female, but also even cause infertility; meanwhile, private skin is sensitive, various inflammations or simple skin itch easily occur, and special nourishing and nursing are needed. With the increase of health consciousness, women have higher requirements for daily care of private parts and prevention and treatment of diseases of private parts.
The common private bath products with cleaning efficacy in the current market can be used for simple cleaning, but cannot solve the problems of peculiar smell, pruritus and the like. For example, in 201610719166.8, the addition of chemical bacteriostatic component is liable to destroy the self-cleaning function of vagina, and may also cause allergy.
Therefore, the development of brand-new products with natural component sources and excellent antibacterial, itching relieving and odor removing effects has important application value.
Disclosure of Invention
The invention aims to provide the plant essence composition with excellent effects of relieving itching and removing odor, the product stability is good, no layering and no precipitation phenomenon exist, the components are highly natural, and the safety is high.
The invention aims to provide a plant essence capable of relieving itching and removing odor.
The invention also aims to provide application of the plant essence for relieving itching and removing odor.
The above purpose of the invention is realized by the following technical scheme:
a plant essence with antipruritic and deodorant effects comprises oleum Cnidii, perilla leaf oil, galla chinensis extract, radix Sophorae Flavescentis extract, semen Armeniacae amarum extract, cortex moutan extract, cortex Phellodendri extract, mentholum, and Borneolum Syntheticum.
The research shows that the different types of the essential oil and the extract and the different combinations of the antipruritic and deodorizing performances have larger difference, and the research shows that the combined effect of the cnidium fruit oil, the perilla leaf oil, the Chinese gall extract, the sophora flavescens extract, the almond extract, the peony root and bark extract, the phellodendron bark extract, the menthol crystal and the borneol is obviously improved.
Preferably, the mass ratio of the cnidium fruit oil to the total amount of the plant essence is 0.5-2g/kg, the perilla leaf oil is 0.1-2g/kg, the Chinese gall extract is 1-5g/kg, the radix sophorae flavescentis extract is 0.01-0.1g/kg, the almond extract is 0.01-0.1g/kg, the peony root bark extract is 0.01-0.1g/kg, the phellodendron bark extract is 0.001-0.01g/kg, the menthol is 3-6g/kg, and the borneol is 0.1-0.5g/kg.
More preferably, the mass ratio of the cnidium fruit oil to the total amount of the plant essence liquid is 0.8-1g/kg, the perilla leaf oil is 0.5-0.8g/kg, the Chinese gall extract is 1.5-2g/kg, the radix sophorae flavescentis extract is 0.03-0.06g/kg, the almond extract is 0.03-0.06g/kg, the peony root and bark extract is 0.03-0.06g/kg, the phellodendron extract is 0.003-0.006g/kg, the menthol is 4-5g/kg, and the borneol is 0.1-0.3g/kg.
Most preferably, the mass ratio of the cnidium fruit oil to the total amount of the plant essence is 0.9g/kg, the perilla leaf oil is 0.6g/kg, the Chinese gall extract is 1.8g/kg, the radix sophorae flavescentis extract is 0.05g/kg, the almond extract is 0.05g/kg, the peony root bark extract is 0.05g/kg, the phellodendron bark extract is 0.005g/kg, the menthol is 4.5g/kg, and the borneol is 0.2g/kg.
The female menstrual period is in a muggy and humid environment, is easy to breed microorganisms, is infected with gynecological inflammation, and produces pruritus and peculiar smell. The plant essence composition selects the traditional Chinese medicines with the functions of clearing heat and drying dampness to sterilize and relieve itching. Wherein, the cnidium fruit oil has the functions of eliminating dampness, dispelling wind, killing parasites and relieving itching; perilla leaf oil has effects of promoting qi circulation and dispelling cold; the gallnut extract has the effects of reducing pathogenic fire and eliminating dampness; the sophora flavescens extract can clear heat, dry dampness and kill parasites; the almond extract has the effects of resisting inflammation, relieving pain and the like; the peony root bark has the functions of calming and relaxing muscles; the phellodendron bark extract has the effects of clearing heat, drying dampness, purging fire and removing toxicity. Modern pharmacological studies show that the phellodendron extract has an inhibiting effect on enterobacteria, staphylococcus aureus, gonococcus and the like, and the gallnut extract has an inhibiting effect on candida albicans.
Further preferably, the solvent is ethanol.
More preferably, the solvent is 60-80% ethanol.
Experiments show that the stability of the product can be maintained by diluting with 70% ethanol, borneol can be separated out when the concentration is too low, the components are not easy to dissolve, and the conditions of turbidity and poor stability can occur when the concentration is too high or the bottom is too high.
Most preferably, therefore, the solvent is 70% ethanol.
Preferably, the preparation method of the plant essence comprises the following steps:
(1) Dissolving Galla chinensis extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(2) Dissolving radix Sophorae Flavescentis extract and cortex Phellodendri extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(3) Dissolving cnidium fruit oil, perilla leaf oil, almond extract and peony root bark extract in 70% ethanol, and mixing;
(4) Dissolving Mentholum and Borneolum Syntheticum in 70% ethanol, and ultrasonic treating to dissolve completely;
(5) And (3) uniformly mixing the solutions obtained in the four steps, and adding 70% ethanol for diluting to a constant volume.
Wherein, preferably, the ultrasonic power is 330W, the frequency is 40Hz, and the time is 10min.
The plant essence liquid product has high quality and excellent itching relieving and smell removing effects. Therefore, the application of the plant essence in preparing the product capable of relieving itching and removing odor also belongs to the protection scope of the invention.
The invention has the following beneficial effects:
according to the invention, a group of plant essences and extract combinations with excellent itching relieving and odor removing effects are obtained through research, namely cnidium fruit oil, perilla leaf oil, a gallnut extract, a sophora flavescens extract, an almond extract, a peony root and bark extract, a phellodendron bark extract, menthol and borneol, and the product is good in stability, free of layering and turbid precipitation phenomenon, highly natural in components and high in safety; is suitable for preparing related products and has good application prospect.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way.
The reagents, methods and apparatus employed in the present invention are conventional in the art, except as otherwise indicated.
Unless otherwise indicated, reagents and materials used in the present invention are commercially available.
The following examples are examples in which the main instruments used for the experiments included: a ZG-1 gas collection pump, a hydrogen sulfide detection tube and an ammonia gas detection tube (all Shanghai Kuncui instrument Co., ltd.); model BSCIIB2-1101 biosafety cabinet (Shanghai Riyan decontamination Equipment, inc.); YXQ-50A vertical pressure steam sterilizer (Shanghai Bosch medical bio instruments, inc.); SPX-250B biochemical incubator (Tester instruments, inc. of Tianjin), etc.
The statistical method of the experimental data in the examples is as follows: the measured data is averaged + -SDStatistical analysis data were performed using SPSS statistical software. The homogeneity of variance was checked by the Leven's test method. If homogeneity of variance (P) is satisfied>0.05 Statistical analysis was performed using One-Way analysis of variance (One-Way ANOVA). ANOVA has statistical significance (P is less than or equal to 0.05), and is compared and analyzed by LSD test (parameter method). Variance (P is less than or equal to 0.05), and Kruskal-Wallis test is carried out. Kruskal-WaThe statistical significance of the llis Test (P.ltoreq.0.05) was determined by means of Dunnett's Test (nonparametric method). P<0.05 was considered statistically significant.
Example 1 plant essence
A plant essence: 9g of cnidium fruit oil, 6g of perilla leaf oil, 18g of Chinese gall extract, 0.5g of radix sophorae flavescentis extract, 0.5g of almond extract, 0.5g of peony root-bark extract, 0.05g of golden cypress extract, 45g of menthol and 2g of borneol.
The preparation method of the plant essence comprises the following steps:
(1) Dissolving Galla chinensis extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(2) Dissolving radix Sophorae Flavescentis extract and cortex Phellodendri extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(3) Dissolving cnidium fruit oil, perilla leaf oil, almond extract and peony root bark extract in 70% ethanol, and mixing;
(4) Dissolving Mentholum and Borneolum Syntheticum in 70% ethanol, and ultrasonic treating to dissolve completely;
(5) And (3) uniformly mixing the solutions obtained in the four steps, and adding 70% ethanol for dilution until the total weight is 10kg.
Wherein the ultrasonic power is 330W, the frequency is 40Hz, and the time is 10min.
Example 2 plant essence
A plant essence: 10g of cnidium fruit oil, 8g of perilla leaf oil, 20g of Chinese gall extract, 0.6g of radix sophorae flavescentis extract, 0.6g of almond extract, 0.6g of peony root-bark extract, 0.06g of golden cypress extract, 50g of menthol and 3g of borneol.
The preparation method of the plant essence comprises the following steps:
(1) Dissolving Galla chinensis extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(2) Dissolving radix Sophorae Flavescentis extract and cortex Phellodendri extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(3) Dissolving oleum Cnidii, perilla leaf oil, semen Armeniacae amarum extract, and cortex moutan extract in 70% ethanol, and mixing;
(4) Dissolving Mentholum and Borneolum Syntheticum in 70% ethanol, and ultrasonic treating to dissolve completely;
(5) And (3) uniformly mixing the solutions obtained in the four steps, and adding 70% ethanol for dilution until the total weight is 10kg.
Wherein the ultrasonic power is 330W, the frequency is 40Hz, and the time is 10min.
Example 3 plant essence
A plant essence: 8g of cnidium fruit oil, 5g of perilla leaf oil, 15g of Chinese gall extract, 0.3g of radix sophorae flavescentis extract, 0.3g of almond extract, 0.3g of peony root-bark extract, 0.03g of golden cypress extract, 40g of menthol and 1g of borneol.
The preparation method of the plant essence comprises the following steps:
(1) Dissolving Galla chinensis extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(2) Dissolving radix Sophorae Flavescentis extract and cortex Phellodendri extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(3) Dissolving oleum Cnidii, perilla leaf oil, semen Armeniacae amarum extract, and cortex moutan extract in 70% ethanol, and mixing;
(4) Dissolving Mentholum and Borneolum Syntheticum in 70% ethanol, and ultrasonic treating to dissolve completely;
(5) And (3) uniformly mixing the solutions obtained in the four steps, and adding 70% ethanol for dilution until the total weight is 10kg.
Wherein the ultrasonic power is 330W, the frequency is 40Hz, and the time is 10min.
Example 4 plant essence
A plant essence: 20g of cnidium fruit oil, 20g of perilla leaf oil, 10g of Chinese gall extract, 1g of radix sophorae flavescentis extract, 0.1g of almond extract, 1g of peony root-bark extract, 0.01g of golden cypress extract, 60g of menthol and 1g of borneol.
The preparation method of the plant essence comprises the following steps:
(1) Dissolving Galla chinensis extract in 60% ethanol, and ultrasonic treating to dissolve completely;
(2) Dissolving radix Sophorae Flavescentis extract and cortex Phellodendri extract in 60% ethanol, and ultrasonic treating to dissolve completely;
(3) Dissolving cnidium fruit oil, perilla leaf oil, almond extract and peony root bark extract in 60% ethanol, and mixing;
(4) Dissolving Mentholum and Borneolum Syntheticum in 60% ethanol, and ultrasonic dissolving;
(5) And (3) uniformly mixing the solutions obtained in the four steps, and adding 60% ethanol for dilution until the total weight is 10kg.
Wherein the ultrasonic power is 330W, the frequency is 40Hz, and the time is 10min.
Example 5 plant essence
A plant essence: 5g of cnidium fruit oil, 1g of perilla leaf oil, 50g of Chinese gall extract, 0.1g of radix sophorae flavescentis extract, 1g of almond extract, 0.1g of peony root-bark extract, 0.1g of cortex phellodendri extract, 30g of menthol and 5g of borneol.
The preparation method of the plant essence comprises the following steps:
(1) Dissolving Galla chinensis extract in 80% ethanol, and performing ultrasonic treatment to dissolve completely;
(2) Dissolving radix Sophorae Flavescentis extract and cortex Phellodendri extract in 80% ethanol, and ultrasonic treating to dissolve completely;
(3) Dissolving cnidium fruit oil, perilla leaf oil, almond extract and peony root bark extract in 80% ethanol, and mixing;
(4) Dissolving Mentholum and Borneolum Syntheticum in 80% ethanol, and ultrasonic treating to dissolve completely;
(5) And (3) uniformly mixing the solutions obtained in the four steps, and adding 80% ethanol for dilution until the total weight is 10kg.
Wherein the ultrasonic power is 330W, the frequency is 40Hz, and the time is 10min.
Example 6 taste-removing experiment of plant essence
The experiment shows that the plant essence liquid has the function of removing the odor through the absorption test of the hydrogen sulfide and the ammonia gas. Tea oil is used as a reference substance, and a positive reference medicine is inflammation diminishing and tissue regeneration promoting ointment (production unit: fujian Yanshan pharmacy Co., ltd.).
1. Test method
The odor removing effect evaluation test is carried out in a self-made gas preparation test bottle, and hydrogen sulfide and ammonia gas are used as odor removing objects. The hydrogen sulfide is generated by reacting iron sulfide powder with hydrochloric acid in a closed conical flask and is stored in a wide-mouth bottle; ammonia gas is directly added with ammonia water and then evaporated in a wide-mouth bottle for storage. A certain volume of a control (tea oil), a positive control (antiphlogistic granulation promoting ointment) and the essence of example 1-5 are dripped into filter paper, and the filter paper is placed in a wide-mouth glass bottle filled with hydrogen sulfide or ammonia gas with determined concentration, the concentration of the hydrogen sulfide or ammonia gas in the wide-mouth glass bottle is detected at different time before and after the odor is removed, and the odor removal rate is calculated.
2. Detecting the index
Detecting the concentration of hydrogen sulfide and ammonia gas: 100mL of gas in the wide-mouth bottle is collected by a ZG-1 gas collection pump in a sampling manner, and the concentrations of hydrogen sulfide and ammonia gas in the wide-mouth glass bottle before and after smell removal are respectively detected by utilizing hydrogen sulfide and ammonia gas detection tubes so as to calculate the removal rate of the hydrogen sulfide and the ammonia gas. Clearance = (pre-treatment measurement-post-treatment measurement)/pre-treatment measurement x 100%.
3. The test results are shown in Table 1
TABLE 1
Group of | Hydrogen sulfide clearance (%) | Ammonia gas removal rate (%) |
Reference (tea oil) | 0 | 0 |
Positive control drug (antiphlogistic granulation promoting ointment) | 5.9 | 7.2 |
Example 1 | 37.9 | 21.2 |
Example 2 | 36.9 | 20.8 |
Example 3 | 37.1 | 19.9 |
Example 4 | 36.7 | 20.2 |
Example 5 | 36.5 | 20.7 |
Example 7 anti-itching test of plant essence
1. Incubation period of pruritus
70 mice weighing about 20g were randomly divided into 7 groups of 10 mice each. 12g/kg of Jingfang antipruritic granule (Jingfang antipruritic granule: lizhu group Sichuan Dai pharmaceutical Co., ltd.) was administered to the positive control group, and the same volume (10 ml/kg) of 0.9% sodium chloride solution was administered to the model control group.
1h after administration, the mouse tail was injected intravenously with dextran 1.25mg/kg. The head of the mouse was scratched by the front paw, the trunk of the mouse was scratched by the right paw, and the itching indications were obtained by biting the whole body with the mouth. The incubation time of scratching and the number of scratching times of the mice within 30min are observed and recorded. The mice of examples 1-5 had significantly longer incubation periods for scratching and significantly reduced numbers of itching (p < 0.01) compared to the model placebo. See table 2.
Table 2 effects of skin itch model in mice of each group (n = 10)
Group of | Number of itching (times) | Incubation time for pruritus (min) |
Blank control group | 23.14±6.51** | 7.85±8.45** |
Jingfang antipruritic granule group | 15.27±5.16** | 8.01±2.91** |
Example 1 | 5.89±1.17** | 10.18±9.16** |
Example 2 | 7.01±6.24** | 19.51±8.21** |
Example 3 | 8.82±8.19** | 21.16±6.14** |
Example 4 | 9.43±6.27** | 23.98±7.22** |
Example 5 | 7.65±9.41** | 21.30±8.81** |
Remarking: p is less than 0.01 compared to blank group.
2. Experiment of histamine phosphate-induced skin itch model of guinea pigs
70 male guinea pigs weighing about 180g were randomly divided into 7 groups of 10 animals each. The positive control group was administered 6.04g/kg of Jingfang antipruritic granule, and the model control group was administered the same volume (10 ml/kg) of 0.9% sodium chloride solution, examples 1-5.
After 24 hours of administration, the shaving part of the right instep is rubbed with abrasive paper (by taking the degree of bleeding), histamine phosphate solution (0.01%, 0.02%, 0.03%. 0.2%) is gradually and gradually dripped on the wound surface from low concentration to high concentration 10 minutes after the application, the itching concentration value is counted until the guinea pig licks the right foot back, and compared with a model control group and a positive control group, the itching concentration value of the guinea pig caused by histamine phosphate can be improved in examples 1 to 5, which shows that the plant essence liquid has better antagonistic effect on the pruritus of the guinea pig caused by histamine phosphate. The results are shown in Table 3.
Table 3 effect of plant essences on values of itch-causing concentration in guinea pigs (n = 10)
Remarking: p was less than 0.01 compared to the blank group.
Example 8 stability testing of plant extracts
1. The physicochemical indexes and the microbial index test results of the essence are shown in table 4, and the detection of all sanitary and sensory physicochemical indexes of the essence prepared by the invention meets the standard requirements.
TABLE 4
2. The products of examples 1 to 5 were subjected to stability tests at constant temperature of 50 ℃ and 4 ℃ to-10 ℃ respectively, and the stability of the products after 1 month, 3 months, 6 months and 12 months was recorded, and the acceptable products were those which did not cause precipitation, odor and discoloration and were free from significant changes in viscosity and pH, and the test results are shown in Table 5.
Table 5 stability test results
Remarking: wherein √ represents a normal; x indicates the occurrence of a change in one or more of odor, color, uniformity, viscosity, pH.
Example 9 irritation test of plant essence
1. Skin irritation test
(1) Experimental animals: rabbits, offered by changsha, tianjiu biotechnology limited. The temperature of the animal room is 24-28 ℃ and the relative humidity is 54-60%.
(2) The detection basis is as follows: disinfection Specification 2002 edition 2.3.3.1.
(3) The detection method comprises the following steps:
3 white rabbits, 2 males and 1 female rabbit, the weight of the rabbits is 2.0-2.5kg, the hair on the two sides of the backs of the rabbits is cut 24 hours before the experiment, the hair-removed areas on the left side and the right side are about 3cm multiplied by 3cm, the left side is a medicine-applying area, and the right side is a control area. Respectively weighing 0.5ml of the test substance, smearing the test substance on the skin of the medicine application area, covering the test substance with a layer of non-irritating plastic film, fixing the test substance with a non-irritating adhesive tape, and applying the test substance for 24 hours. The control group was coated with an equal amount of distilled water.
After application 24, the application area is rinsed with warm water to remove residue. Local reactions of the skin were observed at 1h, 24h, and 48h after removal of the test substance, respectively. The experiment was repeated three times and the mean value was taken. The highest skin irritation index is taken, and the level of the skin irritation intensity of the tested substances to the animals is evaluated according to the disinfection technical specification 2002 edition table 2-12.
(4) The results are shown in Table 6: no erythema and edema on the drug-coated area of the animal after 2 hours of drug administration, and no other abnormal phenomena exist in the animal within the observation period of 48 hours. The highest skin irritation index is 0, and the skin in the control area has no abnormal phenomenon.
TABLE 6 results of irritation test
According to the skin irritation intensity grading standard in the 2002 edition of the disinfection technical Specification, the test result of the acute once complete skin irritation of the washing lotion on the rabbit is as follows: has no irritation, and meets the requirements of disinfection technical Specification 2002 edition 2.3.13.1.
2. Vaginal mucosa irritation test
(1) Experimental animals: rabbits, offered by changsha, tianqin biotechnology limited. The temperature of the animal room is 24-28 ℃ and the relative humidity is 54-60%.
(2) The detection basis is as follows: disinfection Specification 2002 edition 2.3.5.
(3) The detection method comprises the following steps:
experimental animals were randomly divided into a toxicant group (1 group) and a control group (2 groups), 3 animals per group, blunt-ended flexible tubes 8cm in length were connected to 2ml syringes, and were filled with test solution for use, one set for each animal.
Fixing the animal on the back, exposing perineum and vaginal orifice, soaking the catheter with the test solution, inserting into vagina gently for 4-5cm, slowly injecting 2ml of test solution with an injector, and withdrawing the catheter to complete contamination. Control animals were treated with normal saline in the same manner.
24h after infection, the animals are killed by an air embolism method, the whole vagina is taken out after laparotomy, the longitudinal incision is carried out, and whether congestion, edema and other manifestations exist is observed. Then, the vagina is put into 10% formalin solution for fixation for more than 24h, tissues at two ends and 3 parts in the center of the vagina are selected for flaking, histopathology examination is carried out after HE staining, and the stimulation reaction is scored according to the vaginal mucosa stimulation reaction scoring standard in the 2002 edition 2.3.5 of the disinfection technical Specification.
(4) And (3) detection results:
the animals in the control group and the infected group have no abnormal food intake, water drinking, defecation and urination, behavioral activities and the like in the later period of treatment. After 24 toxicant exposure, the vaginal mucosa is basically intact and no obvious congestion, edema and other manifestations are seen. The pathological examination result shows that the vaginal mucosa stimulation index of the infected group is 0.75. According to the skin irritation intensity grading standard in the 2002 edition of the technical Specification for disinfection, the test result of the irritation test of the lotion on the vaginal mucosa of the rabbit is as follows: has no irritation, and meets the requirements of disinfection technical Specification 2002 edition 2.3.13.1.
Comparative example
1. Taking the plant essence of example 1 as an example, the experimental group was compounded with different combinations of essential oil combinations, as shown in Table 7
TABLE 7
2. The odor removing effect and stability data of comparative groups 1 to 9 were tested in the methods of examples 1 to 5.
(1) The results of the odor removing effect are shown in table 8 below, and the odor removing effect of the plant essence of the comparative group is significantly inferior to that of the essence of the present invention.
TABLE 8 results of odor removal
Group of | Hydrogen sulfide clearance (%) | Ammonia gas removal rate (%) |
Example 1 | 37.9 | 21.2 |
Comparative group 1 | 6.3 | 6.9 |
Comparative group 2 | 6.2 | 6.8 |
Comparative group 3 | 6.3 | 7.0 |
Comparative group 4 | 7.0 | 6.7 |
Comparative group 5 | 6.9 | 6.5 |
Comparative group 6 | 6.5 | 6.8 |
Comparative group 7 | 6.7 | 6.1 |
Comparative group 8 | 5.7 | 6.3 |
Comparative group 9 | 6.8 | 6.5 |
(2) The stability data are given in table 9 below:
TABLE 9
Remarking: wherein √ represents a normal; x represents the change in one or more of odor, color, uniformity, viscosity, pH.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (7)
1. A plant essence for relieving itching and removing odor special for women is characterized by comprising cnidium fruit oil, perilla leaf oil, chinese gall extract, radix sophorae flavescentis extract, almond extract, peony root bark extract, golden cypress extract, menthol and borneol; calculated by mass ratio of the total amount of the plant essence, 0.5-2g/kg of cnidium fruit oil, 0.1-2g/kg of perilla leaf oil, 1-5g/kg of Chinese gall extract, 0.01-0.1g/kg of radix sophorae flavescentis extract, 0.01-0.1g/kg of almond extract, 0.01-0.1g/kg of peony root bark extract, 0.001-0.01g/kg of phellodendron extract, 3-6g/kg of menthol and 0.1-0.5g/kg of borneol; the solvent is 60-80% ethanol.
2. The plant essence of claim 1, wherein the plant essence comprises, by mass, 0.8-1g/kg of cnidium officinale oil, 0.5-0.8g/kg of perilla leaf oil, 1.5-2g/kg of Chinese gall extract, 0.03-0.06g/kg of sophora flavescens extract, 0.03-0.06g/kg of almond extract, 0.03-0.06g/kg of peony root-bark extract, 0.003-0.006g/kg of phellodendron bark extract, 4-5g/kg of menthol, and 0.1-0.3g/kg of borneol.
3. The plant essence according to claim 2, wherein the plant essence comprises 0.9g/kg of cnidium officinale oil, 0.6g/kg of perilla leaf oil, 1.8g/kg of a gallnut extract, 0.05g/kg of a sophora flavescens extract, 0.05g/kg of an almond extract, 0.05g/kg of a peony root bark extract, 0.005g/kg of a phellodendron bark extract, 4.5g/kg of menthol and 0.2g/kg of borneol by mass ratio of the total amount of the plant essence.
4. The botanical essence of claim 1, wherein the solvent is 70% ethanol.
5. The plant essence of claim 4, which is prepared by the following steps:
(1) Dissolving Galla chinensis extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(2) Dissolving radix Sophorae Flavescentis extract and cortex Phellodendri extract in 70% ethanol, and ultrasonic treating to dissolve completely;
(3) Dissolving oleum Cnidii, perilla leaf oil, semen Armeniacae amarum extract, and cortex moutan extract in 70% ethanol, and mixing;
(4) Dissolving Mentholum and Borneolum Syntheticum in 70% ethanol, and ultrasonic treating to dissolve completely;
(5) And (3) uniformly mixing the solutions obtained in the four steps, and adding 70% ethanol for diluting to a constant volume.
6. The plant essence according to claim 5, wherein the ultrasonic power is 330W, the frequency is 40Hz, and the time is 10min.
7. Use of the plant essence according to any one of claims 1 to 6 for the preparation of a product for relieving itching and removing odor.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010963385.7A CN112022747B (en) | 2020-09-14 | 2020-09-14 | Itching-relieving and odor-removing plant essence and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010963385.7A CN112022747B (en) | 2020-09-14 | 2020-09-14 | Itching-relieving and odor-removing plant essence and application thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN112022747A CN112022747A (en) | 2020-12-04 |
CN112022747B true CN112022747B (en) | 2023-04-14 |
Family
ID=73589864
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010963385.7A Active CN112022747B (en) | 2020-09-14 | 2020-09-14 | Itching-relieving and odor-removing plant essence and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112022747B (en) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102526301B (en) * | 2011-12-30 | 2014-02-12 | 上海豪源生物制品有限公司 | Compound lightyellow sophora root-selinum japenious seed bacteriostatic lotion and preparation method thereof |
-
2020
- 2020-09-14 CN CN202010963385.7A patent/CN112022747B/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN112022747A (en) | 2020-12-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109568206B (en) | Special antibacterial composition and lotion for women and preparation method thereof | |
CN108452295A (en) | A kind of compact gel and preparation method antibacterial, that adjust microecological balance | |
CN107802825A (en) | A kind of Traditional Chinese medicine gel composition antibacterial for vaginal mucosa | |
CN103340816B (en) | Special private care wet tissue for women | |
CN113081928B (en) | Plant antibacterial gel and preparation method and application thereof | |
RU2308951C2 (en) | Complex method for preventing vaginal dysbacteriosis (variants) | |
CN112121100B (en) | Itching-relieving and odor-removing chip, puerpera pad and preparation method of puerpera pad | |
CN112294886A (en) | Foam type lavender antibacterial lotion and preparation method thereof | |
CN112022747B (en) | Itching-relieving and odor-removing plant essence and application thereof | |
CN111973551A (en) | Mussel mucin antibacterial gel and preparation method thereof | |
CN108404111A (en) | One kind is with kidney bean phytolectin inhibitory anti-virus preparation as main component | |
CN107049881A (en) | A kind of dog cat health fragrance involves its preparation method | |
CN107582943B (en) | Compound traditional Chinese medicine transdermal spray for preventing and treating animal sarcoptidosis and preparation method thereof | |
CN105687021A (en) | Foot powder for preventing tinea pedis | |
CN104721668A (en) | Private nursing solution as well as preparation method and use method thereof | |
CN111904887B (en) | Skin hygiene care composition and application thereof in paper diapers | |
CN112121101B (en) | Itching-relieving and odor-removing plant essence microcapsule and preparation method thereof | |
CN106880698A (en) | A kind of plants essential oil and preparation method for treating child's property vaginitis | |
CN110506764A (en) | A kind of farm disinfecting, deodorizing nanoemulsion and preparation method and application | |
CN107737131A (en) | A kind of skin and mucosa damages antibacterial disinfectant and preparation method | |
CN114129493B (en) | Gastrodia elata washing-free antibacterial gel and preparation method and application thereof | |
CN103735494B (en) | External antipruritic ointment for treating mosquito bite and preparation method of external antipruritic ointment | |
CN112206259B (en) | Traditional Chinese medicine composition for treating vaginitis, traditional Chinese medicine lotion and application | |
CN111329790B (en) | Bacteriostatic low-foam lotion for women and preparation method thereof | |
CN114288366B (en) | Medicated bath composition for preventing and treating skin diseases of bedridden patients and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |