CN110506764A - A kind of farm disinfecting, deodorizing nanoemulsion and preparation method and application - Google Patents
A kind of farm disinfecting, deodorizing nanoemulsion and preparation method and application Download PDFInfo
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- CN110506764A CN110506764A CN201910935617.5A CN201910935617A CN110506764A CN 110506764 A CN110506764 A CN 110506764A CN 201910935617 A CN201910935617 A CN 201910935617A CN 110506764 A CN110506764 A CN 110506764A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
- A61L9/013—Deodorant compositions containing animal or plant extracts, or vegetable material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
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- Plant Pathology (AREA)
- Dentistry (AREA)
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Abstract
The present invention provides a kind of farm disinfecting, deodorizing nanoemulsions, belong to livestock and poultry cultivation field.Its technical solution are as follows: nanoemulsion includes effective component and auxiliary material, effective component includes polyhexamethylene guanide or poly (hexamethylene) class, effective component further includes peptide, subtilisin, garlic oil, borneol, Thymol, eugenol, Cineole, menthol, oil of Cymbopogon winterianus, palchouli oil, auxiliary material includes surfactant, cosurfactant, water for pharmaceutical purposes.The invention has the benefit that the preparation method of disinfecting, deodorizing nanoemulsion of the present invention is low in cost, easy to operate and agents useful for same toxicity is smaller; it is lower to the body harm degree of producers; be conducive to safety in production and environmental protection; simultaneously; disinfecting, deodorizing nanoemulsion has the ability of extremely strong killing bacterium and virus; cultivation place peculiar smell, and broad-spectrum high efficacy can be effectively removed, there is long-term bacteriostasis；It is safe and free of toxic and side effects.
Description
Technical field
The present invention relates to a kind of livestock and poultry cultivation field more particularly to a kind of farm disinfecting, deodorizing nanoemulsions.
Background technique
In recent years, as the shortening of culture-cycle, cultivation density improve, it is high-incidence to result in livestock and poultry.Not only to cultivation
Industry brings biggish economic loss, more seriously threatens food safety and human health.Only effectively prevention, just can be reduced hair
Disease and death.Disinfection is the key link for implementing the important measures puted prevention first and all kinds of livestock and poultries of prevention and control.Many diseases
Disease is precisely due to sterilizing lack of standardization and being not thorough caused.Animal husbandry stench is grown place mainly from the fecaluria of animal and poultry and is rushed
Wash waste water, fresh poultry, livestock waste physico-chemical property and odorant, the air pollutants distributed especially stink often makes
Complained at neighbouring neighbouring residents from villages and towns, cause the puzzlement of resident, livestock-raising field and government bodies, the staff of farm by
Evil is more so.
Livestock and poultry farm disinfection should mainly around feeding, drinking-water, air, poultry house, production district, vehicle apparatus, personnel,
Waste, sewage, animal carcass etc. carry out.Selection disinfectant should follow the original of " efficient, safe and stable, economic, conveniently "
Then.Selected disinfectant should have powerful sterilization and ability of killing the virus；Safety non-toxic and residual, irritation is small, nothing
Special stink and color；The validity period of medicament is long and stablizes, with other disinfectants without incompatibility；It is cheap, user
Just, it is easy to get；Deodorizing effect should rapidly effectively, and effect is lasting.
Summary of the invention
Low in cost, easy to operate and agents useful for same toxicity that the purpose of the present invention is to provide a kind of preparation methods compared with
It is small, it is lower to the body harm degree of producers, be conducive to safety in production and environmental protection, meanwhile, disinfecting, deodorizing nano-emulsion
Agent has the ability of extremely strong killing bacterium and virus, and broad-spectrum high efficacy, can effectively remove cultivation place peculiar smell, has long-term
Bacteriostasis；Farm safe and free of toxic and side effects disinfecting, deodorizing nanoemulsion.
The present invention is realized by following measure:
A kind of farm's disinfecting, deodorizing nanoemulsion, which is characterized in that the nanoemulsion includes effective component and auxiliary material, institute
Stating effective component includes 1-30 parts of polyhexamethylene guanide or 1-30 parts of poly (hexamethylene) class, and the effective component further includes contracting
1-25 parts of propylhomoserin, 1-25 parts of subtilisin, 1-15 parts of garlic oil, 1-15 parts of borneol, 1-15 parts of Thymol, eugenol 1-10
Part, 1-10 parts of Cineole, 1-10 parts of menthol, 1-10 parts of oil of Cymbopogon winterianus, 1-10 parts of palchouli oil, the auxiliary material includes surface-active
5-20 parts of agent, 1-20 parts of cosurfactant, 5-20 parts of water for pharmaceutical purposes.
Specific feature of the invention is also:
The nanoemulsion includes effective component and auxiliary material, and the effective component includes 15 parts of polyhexamethylene guanide, described effective
Ingredient further include 17 parts of peptide, 16 parts of subtilisin, 8 parts of garlic oil, 7 parts of borneol, 7 parts of Thymol, 6 parts of eugenol,
7 parts of Cineole, 8 parts of menthol, 6 parts of oil of Cymbopogon winterianus, 6 parts of palchouli oil, the auxiliary material include 13 parts of surfactant, help surface living
14 parts of agent, 14 parts of water for pharmaceutical purposes of property.
The nanoemulsion includes effective component and auxiliary material, and the effective component includes 10 parts of poly (hexamethylene) class,
The effective component further includes 13 parts of peptide, 12 parts of subtilisin, 5 parts of garlic oil, 6 parts of borneol, 5 parts of Thymol, fourth
4 parts of fragrant phenol, 6 parts of Cineole, 4 parts of menthol, 3 parts of oil of Cymbopogon winterianus, 5 parts of palchouli oil, the auxiliary material include 11 parts of surfactant,
8 parts of cosurfactant, 13 parts of water for pharmaceutical purposes.
The nanoemulsion is manufactured as follows:
S1, appropriate polyhexamethylene guanide or poly (hexamethylene) class is taken to be dissolved in water for pharmaceutical purposes, it is spare；
S2, appropriate peptide, subtilisin, garlic oil, borneol, Thymol, eugenol, Cineole, menthol, lemongrass are taken
Essential oil, palchouli oil, surfactant, cosurfactant, are mixed evenly, spare；
S3, step S1 and the finally obtained solution of step S2 are mixed evenly and are put into high-pressure homogeneous instrument, grain is prepared
Diameter is the nano-emulsion of 10~100 nm.
The surfactant is stearic acid sodium, potassium oleate, lauryl sodium sulfate, calcium stearate, polyglycereol stearic acid
Rouge, polyglycereol olein, sapn (20,40,60,80), polyoxyethylene fatty acid ester (45,49,52), ethoxylated dodecyl alcohol
(30,35) poloxamer, tween (20,40,60,80), Arabic gum, tragacanth, gelatin, apricot glue, one in cholesterol
Kind is a variety of.
The cosurfactant be ethyl alcohol, normal propyl alcohol, isopropanol, n-butanol, isobutanol, n-amyl alcohol, isoamyl alcohol, 1- oneself
One of alcohol, 2- hexanol, 1- octanol, sec-n-octyl alcohol, fusel oil, n nonylphenol are a variety of.
The nanoemulsion is in the application of disinfection and deodorization in livestock and poultry cultivation place.
The invention has the benefit that the preparation method of disinfecting, deodorizing nanoemulsion of the present invention it is low in cost, it is easy to operate,
And agents useful for same toxicity is smaller, lower to the body harm degree of producers, is conducive to safety in production and environmental protection, together
When, disinfecting, deodorizing nanoemulsion has the ability of extremely strong killing bacterium and virus, cultivation place peculiar smell can be effectively removed, and
Broad-spectrum high efficacy has long-term bacteriostasis；It is safe and free of toxic and side effects.
Specific embodiment
In order to clarify the technical characteristics of the invention, being illustrated below by specific embodiment to this programme.
Embodiment 1:
The nanoemulsion includes effective component and auxiliary material, and the effective component includes 5 parts of polyhexamethylene guanide, it is described effectively at
Dividing further includes 4 parts of peptide, 5 parts of subtilisin, 3 parts of garlic oil, 4 parts of borneol, 6 parts of Thymol, 5 parts of eugenol, folium eucalypti
Plain 4 parts, 3 parts of menthol, 3 parts of oil of Cymbopogon winterianus, 3 parts of palchouli oil, the auxiliary material includes 9 parts of surfactant, cosurfactant 8
Part, 7 parts of water for pharmaceutical purposes.
The nanoemulsion includes effective component and auxiliary material, and the effective component includes 5 parts of poly (hexamethylene) class, institute
Stating effective component further includes 4 parts of peptide, 5 parts of subtilisin, 3 parts of garlic oil, 4 parts of borneol, 6 parts of Thymol, eugenol 5
Part, 4 parts of Cineole, 3 parts of menthol, 3 parts of oil of Cymbopogon winterianus, 3 parts of palchouli oil, the auxiliary material include 9 parts of surfactant, help surface
8 parts of activating agent, 7 parts of water for pharmaceutical purposes.
The nanoemulsion is manufactured as follows:
S1, appropriate polyhexamethylene guanide or poly (hexamethylene) class is taken to be dissolved in water for pharmaceutical purposes, it is spare；
S2, appropriate peptide, subtilisin, garlic oil, borneol, Thymol, eugenol, Cineole, menthol, lemongrass are taken
Essential oil, palchouli oil, surfactant, cosurfactant, are mixed evenly, spare；
S3, step S1 and the finally obtained solution of step S2 are mixed evenly and are put into high-pressure homogeneous instrument, grain is prepared
Diameter is the nano-emulsion of 10~100 nm.
The nanoemulsion of this test preparation is during storage 24 months, in appearance character, identification, inspection, the inspection of content items
It looks into index to have no significant change, meets every regulation of preclinical drug standard, quality is basicly stable.
Embodiment 2
The nanoemulsion includes effective component and auxiliary material, and the effective component includes 15 parts of polyhexamethylene guanide, described effective
Ingredient further include 17 parts of peptide, 16 parts of subtilisin, 8 parts of garlic oil, 7 parts of borneol, 7 parts of Thymol, 6 parts of eugenol,
7 parts of Cineole, 8 parts of menthol, 6 parts of oil of Cymbopogon winterianus, 6 parts of palchouli oil, the auxiliary material include 13 parts of surfactant, help surface living
14 parts of agent, 14 parts of water for pharmaceutical purposes of property.
The nanoemulsion includes effective component and auxiliary material, and the effective component includes 10 parts of poly (hexamethylene) class,
The effective component further includes 13 parts of peptide, 12 parts of subtilisin, 5 parts of garlic oil, 6 parts of borneol, 5 parts of Thymol, fourth
4 parts of fragrant phenol, 6 parts of Cineole, 4 parts of menthol, 3 parts of oil of Cymbopogon winterianus, 5 parts of palchouli oil, the auxiliary material include 11 parts of surfactant,
8 parts of cosurfactant, 13 parts of water for pharmaceutical purposes.
The nanoemulsion is manufactured as follows:
S1, appropriate polyhexamethylene guanide or poly (hexamethylene) class is taken to be dissolved in water for pharmaceutical purposes, it is spare；
S2, appropriate peptide, subtilisin, garlic oil, borneol, Thymol, eugenol, Cineole, menthol, lemongrass are taken
Essential oil, palchouli oil, surfactant, cosurfactant, are mixed evenly, spare；
S3, step S1 and the finally obtained solution of step S2 are mixed evenly and are put into high-pressure homogeneous instrument, grain is prepared
Diameter is the nano-emulsion of 10~100 nm.
The nanoemulsion of this test preparation is during storage 24 months, in appearance character, identification, inspection, the inspection of content items
It looks into index to have no significant change, meets every regulation of preclinical drug standard, quality is basicly stable.
The inventor of nanoemulsion of the present invention has carried out a series of experiments and has ground in terms of the preparation method and applications of raw material
Study carefully, it is determined that the preparation method and its advantages of preparation nanoemulsion of the present invention, making it compared with prior art has obviously
Disinfecting, deodorizing advantageous effect.
Test one, nanoemulsion clinical trial of the present invention
1, irritation test
The New Zealand White rabbit of acute eye irritation test healthy adult 4,2.5 ~ 3.0kg of weight, by disinfecting, deodorizing cream
0.1ml instillation rabbit conjunctiva of left eye is intracapsular, and normal saline flushing is used after passive closure 4s, and right eye is to compare drop physiological saline.In
6 after eye drip, 24,48,72h and 4,7d observe and record a local stimulation and react and score.
Acute skin irritation test takes New Zealand White rabbit 4 of healthy adult, and 2.5 ~ 3.0kg of weight cuts off backbone
The hair of two sides, going to gross area two sides is respectively 4cm × 4cm, after confirming no skin injury afterwards for 24 hours, the disinfecting, deodorizing cream of 0.5ml is straight
Application is connect on the rabbit back side skin for going to hair-fields, is then covered with two layers of gauze and one layer of glassine paper, non-stimulated immobilization with adhesive tape,
Other side skin is as blank control.Wash away residue with warm water after fixed 4h, after observation removal tested material 24, the skin of 48h
Skin stimulate the reaction calculates PiI, makes evaluation.
Irritant test the result shows that, the disinfecting, deodorizing cream to the acute eye irritation score index of animal be 3, have slight thorn to eye
Swash property.It is 0 to rabbit skin irritatin intensity value, belongs to nonirritant.
2, corrosion test
Diameter 25.0mm is made in 4 kinds of sheet metals such as stainless steel, carbon steel, copper and aluminium, thick 1.0mm is round, and wearing a diameter is 2.0mm
Aperture, surface area total value are about 9.81cm2, finish 8.It is impregnated, is deoiled with cleaning detergent, cleaned；With No. 120 granularity water
Sand paper grinds off the oxide layer of metal surface, then is washed down with tap water, sets in 50 DEG C of insulating boxs and is precisely weighed after dry 3h.With each
The disinfecting, deodorizing cream is dispensed into beaker by sheet metal 200ml dosage, and every kind 1, metal；Then one formula 3 of every kind of sheet metal is taken
Piece suspension is immersed in 72h in disinfecting, deodorizing cream, after taking out sheet metal removing corrosion product and cleaning, is precisely weighed conduct after dry
Weight after test.Calculate corrosion rate.Through detecting, compound disinfecting cream stoste impregnates 72h to 4 kinds of metals, to stainless steel, copper without corruption
Corrosion, < 0.00100 to carbon steel, this corrosion rate of aluminium base R (mm/a).
3, toxicity test
Acute oral toxicity test selects the Kunming kind level-one mouse 20 of 20 ~ 22g of weight, half male and half female.It is adopted with disinfecting, deodorizing cream
With primary oral gastric infusion, 14d is observed after administration, records mouse various symptoms and dead animal number, calculates LD50Value.
Cumulative toxicity test takes the Kunming kind level-one rat 20 of 180 ~ 200g of weight, half male and half female.According to acute toxicity
Test LD50Poisoning dosage, 1~4d poisoning dosage are 0.1LD to value according to the weight50, later incremental 1.5 multiple doses of every 4h, directly
To 28d.Every 4h weighs 1 time, calculates cumulative coefficient.
Mouse marrow cell micro nuclear test selects healthy level-one mouse 50, is randomly divided into 5 groups, every group of male and female each 5.
Design 1/2LD50, 1/5LD50, 1/20LD503 dosage groups of mg/kg weight, separately set negative control (distilled water) and positive control
[giving cyclophosphamide 40mg/kg (weight)].Oral gastric infusion, for 24 hours using dosing interval twice, in 6h after the last administration, often
Rule take femur bone marrow film-making, fixation, dyeing, observe 1000 polychromatic erythrocytes to every animal under oil mirror, calculate micronucleus
Rate carries out statistical analysis.
Mouse inbred strain selects 25 male and healthy mouse, is randomly divided into 5 groups, every group 5.Design 1/2,1/
5,1/20LD50, 3 dosage groups are waited, separately set negative control (distilled water) and positive control [to cyclophosphamide 40mg/kg (body
Weight)].Oral gastric infusion, continuous 5d, the 35d after first administration put to death animal, take epididymis film-making, fixation, dyeing, in
Defective sperm number in 2000 sperms is observed to every animal under oil mirror, calculates Sperm malformation rate, carries out statistical analysis.
Test result shows that in acute oral toxicity test, no abnormality seen reacts after mouse contamination, does not occur death,
Compound disinfectant LD oral to chmice acute50> 5000mg/kg (weight), true border are non-toxic.Cumulative toxicity test administration
20d, female, male mouse occur without death condition, mouse cumulative coefficient K > 5, are weak cumulative toxicity, and the thermophilic polychromatophilia of mouse bone marrow cells is red thin
Born of the same parents' micronucleus test is feminine gender, no mutagenicity.Sperm malformation test result is feminine gender.
Experiment two, nanoemulsion quality testing of the present invention experiment
Experimental example of the present invention carries out quality evaluation to nano-emulsion made from embodiment 1-2.
Evaluation method:
1, structure type takes nano-emulsion appropriate, and 4 × 103r·min-1It is centrifuged 10min, observes emulsion droplet state.
2, morphologic observation and particle diameter distribution distilled water dilute fatty nano-emulsion, then plus OsO4Solution fixes 15 min, will
Fixed pellicle does TEM measurement.
3, stability includes that strong illumination test, high humility test, hot test, accelerated test, room temperature keep sample investigation.
Evaluation result:
1, structure type is clear and bright nano-emulsion.
2, morphologic observation and particle diameter distribution form are uniform, and particle diameter distribution is 30 ~ 80nm, are evenly distributed.
3, stability is through study on the stability, product characteristics stable uniform.
Test three, nanoemulsion application experiment of the present invention
1, disinfecting, deodorizing merit rating
Further verifying is of the invention in terms of cultivating place disinfecting, deodorizing by the test of farm's disinfecting, deodorizing for experimental example of the present invention
Validity.
(1) sterilization test
According to 2017 editions " disinfection technology standard " predetermined operation methods: nanoemulsion is diluted according to the ratio of 1:200, it is right
Staphylococcus aureus, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Salmonella enteritidis, proteus vulgaris, corynebacterium diphtheriae,
Shigella flexneri, pseudomonas aeruginosa have carried out killing test.
1 disinfecting, deodorizing nanoemulsion bacterial inactivation rate of table
| Sample | Staphylococcus aureus | Escherichia coli | Candida albicans | Pseudomonas aeruginosa | Salmonella enteritidis | Proteus vulgaris | Corynebacterium diphtheriae | Shigella flexneri | Pseudomonas aeruginosa |
| Nanoemulsion | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 99 |
2, deodorizing capability is tested
The present invention is applied to several farms, carries out field test, cultivation place staff generally reacts, farm
Institute's peculiar smell substantially reduces, and effect can continue 3 ~ 5 days.
Claims (7)
1. a kind of farm disinfecting, deodorizing nanoemulsion, which is characterized in that the nanoemulsion includes effective component and auxiliary material,
The effective component includes 1-30 parts of polyhexamethylene guanide or 1-30 parts of poly (hexamethylene) class, and the effective component further includes
1-25 parts of peptide, 1-25 parts of subtilisin, 1-15 parts of garlic oil, 1-15 parts of borneol, 1-15 parts of Thymol, eugenol 1-
10 parts, 1-10 parts of Cineole, 1-10 parts of menthol, 1-10 parts of oil of Cymbopogon winterianus, 1-10 parts of palchouli oil, the auxiliary material include that surface is living
Property 5-20 parts of agent, 1-20 parts of cosurfactant, 5-20 parts of water for pharmaceutical purposes.
2. farm according to claim 1 disinfecting, deodorizing nanoemulsion, which is characterized in that the nanoemulsion includes
Effective component and auxiliary material, the effective component include 15 parts of polyhexamethylene guanide, the effective component further include 17 parts of peptide,
16 parts of subtilisin, 8 parts of garlic oil, 7 parts of borneol, 7 parts of Thymol, 6 parts of eugenol, 7 parts of Cineole, 8 parts of menthol, perfume
6 parts of thatch essential oil, 6 parts of palchouli oil, the auxiliary material include 13 parts of surfactant, 14 parts of cosurfactant, and 14 parts of water for pharmaceutical purposes.
3. farm according to claim 1 disinfecting, deodorizing nanoemulsion, which is characterized in that the nanoemulsion includes
Effective component and auxiliary material, the effective component include 10 parts of poly (hexamethylene) class, and the effective component further includes peptide
13 parts, 12 parts of subtilisin, 5 parts of garlic oil, 6 parts of borneol, 5 parts of Thymol, 4 parts of eugenol, 6 parts of Cineole, menthol 4
Part, 3 parts of oil of Cymbopogon winterianus, 5 parts of palchouli oil, the auxiliary material include 11 parts of surfactant, 8 parts of cosurfactant, water for pharmaceutical purposes
13 parts.
4. farm according to claim 1 disinfecting, deodorizing nanoemulsion, which is characterized in that the nanoemulsion be by
Made of following method:
S1, appropriate polyhexamethylene guanide or poly (hexamethylene) class is taken to be dissolved in water for pharmaceutical purposes, it is spare；
S2, appropriate peptide, subtilisin, garlic oil, borneol, Thymol, eugenol, Cineole, menthol, lemongrass are taken
Essential oil, palchouli oil, surfactant, cosurfactant, are mixed evenly, spare；
S3, step S1 and the finally obtained solution of step S2 are mixed evenly and are put into high-pressure homogeneous instrument, grain is prepared
Diameter is the nano-emulsion of 10~100 nm.
5. farm according to claim 1 disinfecting, deodorizing nanoemulsion, which is characterized in that the surfactant is
Stearic acid sodium, potassium oleate, lauryl sodium sulfate, calcium stearate, polyglycereol stearate, polyglycereol olein, sapn (20,
40,60,80), polyoxyethylene fatty acid ester (45,49,52), ethoxylated dodecyl alcohol (30,35) poloxamer, tween (20,
40,60,80), Arabic gum, tragacanth, gelatin, apricot glue, one of cholesterol or a variety of.
6. farm according to claim 1 disinfecting, deodorizing nanoemulsion, which is characterized in that the cosurfactant
For ethyl alcohol, normal propyl alcohol, isopropanol, n-butanol, isobutanol, n-amyl alcohol, isoamyl alcohol, 1- hexanol, 2- hexanol, 1- octanol, sec-n-octyl alcohol,
One of fusel oil, n nonylphenol are a variety of.
7. farm according to claim 1 disinfecting, deodorizing nanoemulsion, which is characterized in that the nanoemulsion
The application of disinfection and deodorization in livestock and poultry cultivation place.
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| CN201910935617.5A CN110506764A (en) | 2019-09-29 | 2019-09-29 | A kind of farm disinfecting, deodorizing nanoemulsion and preparation method and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110876811A (en) * | 2019-12-03 | 2020-03-13 | 深圳市三林生物科技工程有限公司 | Water-soluble plant extract deodorant and preparation method thereof |
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| CN102870797A (en) * | 2011-07-15 | 2013-01-16 | 上海泽旭生物科技有限公司 | Sterilizing deodorant |
| CN105028452A (en) * | 2015-05-19 | 2015-11-11 | 沈阳光彩生物科技有限公司 | Air disinfecting spray solution and preparation method of same |
| CN108079354A (en) * | 2017-07-28 | 2018-05-29 | 江门市和馨香精技术有限公司 | A kind of air purifying composition, its preparation method and application |
| CN108782628A (en) * | 2018-07-30 | 2018-11-13 | 合肥华盖生物科技有限公司 | A kind of pure and fresh disinfectant of environment-friendly type air and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102870797A (en) * | 2011-07-15 | 2013-01-16 | 上海泽旭生物科技有限公司 | Sterilizing deodorant |
| CN105028452A (en) * | 2015-05-19 | 2015-11-11 | 沈阳光彩生物科技有限公司 | Air disinfecting spray solution and preparation method of same |
| CN108079354A (en) * | 2017-07-28 | 2018-05-29 | 江门市和馨香精技术有限公司 | A kind of air purifying composition, its preparation method and application |
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