CN111996164A - 一种间充质干细胞的无血清抗衰老培养基 - Google Patents

一种间充质干细胞的无血清抗衰老培养基 Download PDF

Info

Publication number
CN111996164A
CN111996164A CN202010945311.0A CN202010945311A CN111996164A CN 111996164 A CN111996164 A CN 111996164A CN 202010945311 A CN202010945311 A CN 202010945311A CN 111996164 A CN111996164 A CN 111996164A
Authority
CN
China
Prior art keywords
mesenchymal stem
stem cells
serum
culture medium
aging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010945311.0A
Other languages
English (en)
Inventor
不公告发明人
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Liaocheng people's hospital
Original Assignee
Liaocheng people's hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Liaocheng people's hospital filed Critical Liaocheng people's hospital
Priority to CN202010945311.0A priority Critical patent/CN111996164A/zh
Publication of CN111996164A publication Critical patent/CN111996164A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

本文发明公开了一种间充质干细胞抗衰老的无血清培养液。本发明的培养基以DMEM/F12为基础培养基，外源添加物为人成纤维细胞生长因子、人表皮生长因子、胰岛素、L‑谷氨酰胺、巯基乙醇、转铁蛋白、人血清白蛋白、纤粘连蛋白、血小板衍生因子、地塞米松、维生素D3、维生素C、甘草次酸、大蒜素。本发明的无血清培养基成分明确，质量可控，可延缓间充质干细胞的老化速率，保持间充质干细胞的活性和功能，可促进间充质干细胞的产业化进程。

Description

一种间充质干细胞的无血清抗衰老培养基
技术领域
本发明涉及干细胞培养领域，特别涉及无血清培养基及其制备方法和间充质干细胞的培养方法。
背景技术
干细胞及其代表的再生医学领域研究近二十年来不断取得重大技术突破，干细胞产业及临床应用引起了高度重视。间充质干细胞由于具有多种优点成为研究与应用最为广泛的干细胞。间充质干细胞一类具有自我复制和多向分化能力的细胞，广泛存在于骨髓、脂肪、脐带、脐带血和胎盘等组织。间充质干细胞具有取材方便，来源广泛，无伦理学限制等优势，还具有自我更新、多向分化能力和免疫调控作用，可作为理想的种子细胞，近年来国内外已开展间充质干细胞的多项临床研究。
传统的人间充质干细胞培养基多含胎牛血清，血清能为干细胞提供生长增殖所需的激素、生长因子、转移蛋白和其它营养物质。但胎牛血清为动物来源物质，成分复杂且含有异种蛋白，存在批次产品差异大、来源不稳定，还易被病毒和支原体感染，阻碍了间充质干细胞临床转化应用。临床应用的干细胞制剂制备所用的培养基成分应有足够的纯度并符合无菌、无致病微生物及内毒素的质量标准，残留的培养基对受者应无不良影响；在满足干细胞正常生长的情况下，不影响干细胞的的“干性”及分化能力。因此，成分确定且质量可控的抗衰老的无血清培养基是临床应用MSC培养的必然选择。
发明内容
本发明的目的是为了克服现有技术的不足，提供了一种间充质干细胞抗衰老的培养液及制备方法，在提高间充质干细胞抗衰老的同时，保持间充质干细胞的活性和功能，提高利用效率，满足临床应用的需求。本发明的目的将通过下面的详细描述进一步解释和说明。
本发明的目的在于提供一种间充质干细胞的无血清抗衰老培养基，包括DMEM/12基础培养基，还包括如下组分：人成纤维细胞生长因子、人表皮生长因子、胰岛素、L- 谷氨酰胺、巯基乙醇、转铁蛋白、人血清白蛋白、纤粘连蛋白、血小板衍生因子、地塞米松、维生素D3、维生素C、甘草次酸、大蒜素。
本发明提供的间充质干细胞的无血清抗衰老培养基，包括DMEM/12基础培养基，还包括如下组分及其浓度。
Figure BDA0002675150030000021
优选地，本发明提供的间充质干细胞的无血清抗衰老培养基，包括DMEM/12基础培养基，还包括如下组分及其浓度。
Figure BDA0002675150030000022
Figure BDA0002675150030000031
优选地，本发明所述的间充质干细胞为脐带间充质干细胞。
本发明的无血清培养基成分简单清晰，无批次之间的差异，重复性好，避免了异种物质，减少了细胞工艺操作过程中杂质的去除过程，且产品稳定，利于间充质干细胞的产业转化。
具体实施方式
下面结合实例对本发明做进一步详细说明。
下述实施例中的实验方法，如无特别说明，均为常规方法。实验所用仪器药品试剂皆可通过商业途径获得。本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明的一部分而不是全部的实施例。基于本发明中的实施例，本领域的研究人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。本发明提供的培养液，发挥传统中医药优势，与现代科学有机结合，创造性添加了两种传统中药成分。
实施例1
本实施例的一种间充质干细胞的无血清抗衰老培养基，以DMEM/12培养基为基础培养基，还包括以下添加成分：人成纤维细胞生长因子为10ng/ml、人表皮生长因子为10ng/ml、胰岛素为10μg/mL、谷氨酰胺为5mM、巯基乙醇为20μM、转铁蛋白为15μg/ml、人血清白蛋白为50mg/mL、纤粘连蛋白为10ng/ml、血小板衍生生长因子为5ng/ml、地塞米松为5μg/L、维生素D3为2mg/mL、维生素C为25μg/ml、甘草次酸为25nM、大蒜素为20ng/ml。
其具体配制方法是在购买的DMEM/12成品培养基中添加上述成分，并使浓度符合上述浓度，再用无菌碳酸氢钠缓冲溶液调节pH至7.2-7.4，由此得到间充质干细胞的无血清抗衰老培养基。
实施例2
本实施例的一种间充质干细胞的无血清抗衰老培养基，以DMEM/12培养基为基础培养基，还包括以下添加成分：人成纤维细胞生长因子为5ng/ml、人表皮生长因子为5 ng/ml、胰岛素为5μg/mL、谷氨酰胺为10mM、巯基乙醇为10μM、转铁蛋白为20μg/ml、人血清白蛋白为40mg/mL、纤粘连蛋白为5ng/ml、血小板衍生生长因子为8ng/ml、地塞米松为4μg/L、维生素D3为4mg/mL、维生素C为20μg/ml、甘草次酸为20nM、大蒜素为25ng/ml。
其具体配制方法是在购买的DMEM/12成品培养基中添加上述成分，并使浓度符合上述浓度，再用无菌碳酸氢钠缓冲溶液调节pH至7.2-7.4，由此得到间充质干细胞的无血清抗衰老培养基。
试验例效果检测
1、表面标志检测
取冻存的P1代的脐带间充质干细胞，进行常规复苏后，采用胎牛血清培养基及实施例1和实施例2无血清培养基，进行培养，连续传代3次。可见细胞均呈梭形、贴壁生长良好。取P4代细胞进行表面分子检测结果，三组间无显著差异，具体结果见下表
Figure BDA0002675150030000041
2、增值速率检测
取P4代细胞，按照1×104个/孔接种在24孔板中，置于37℃、5％CO2的培养箱培养。在第1、3、5天收集细胞进行计数，每次随机收集3个孔。试验结果表明血清组比实施例1和2增殖速率稍高，差异无统计学意义，基本相当。
Figure BDA0002675150030000042

Claims (4)

1.一种间充质干细胞抗衰老的无血清培养基，其特征在于，包括DMEM/F12基础培养基，还包括如下分组成：人成纤维细胞生长因子、人表皮生长因子、胰岛素、L-谷氨酰胺、巯基乙醇、转铁蛋白、人血清白蛋白、纤粘连蛋白、血小板衍生因子、地塞米松、维生素D3、维生素C、甘草次酸、大蒜素。
2.根据权利要求1所述的无血清培养基，其特征在于，以DMEM/F12为基础培养基，各外源添加物成分用量如下：
Figure FDA0002675150020000011
。
3.根据权利要求2所述的无血清培养基，其特征在于，以DMEM/F12为基础培养基，各外源添加物成分用量如下：
Figure FDA0002675150020000012
Figure FDA0002675150020000021
。
4.根据权利要求1所述的培养方法，其特征在于，所述间充质干细胞为脐带间充质干细胞。
CN202010945311.0A 2020-09-10 2020-09-10 一种间充质干细胞的无血清抗衰老培养基 Pending CN111996164A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010945311.0A CN111996164A (zh) 2020-09-10 2020-09-10 一种间充质干细胞的无血清抗衰老培养基

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010945311.0A CN111996164A (zh) 2020-09-10 2020-09-10 一种间充质干细胞的无血清抗衰老培养基

Publications (1)

Publication Number Publication Date
CN111996164A true CN111996164A (zh) 2020-11-27

Family

ID=73468611

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010945311.0A Pending CN111996164A (zh) 2020-09-10 2020-09-10 一种间充质干细胞的无血清抗衰老培养基

Country Status (1)

Country Link
CN (1) CN111996164A (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114107193A (zh) * 2021-12-14 2022-03-01 中国人民解放军联勤保障部队第九二〇医院 一种人脐带间充质干细胞的无分化扩增抗衰老培养基

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1863906A (zh) * 2000-11-22 2006-11-15 杰龙公司 多功能干细胞的耐受性同种异体移植物
US20070122906A1 (en) * 2003-12-29 2007-05-31 Allan Mishra Method of culturing cells
WO2008100083A1 (en) * 2007-02-14 2008-08-21 Prostemics Co., Ltd. A composition of the injectable agents for tissues repaire containing mesenchymal stem cells and optimized culture media
US20090202496A1 (en) * 2005-07-28 2009-08-13 Eden Biotech Ltd. Method for regenerating an immune system
CN109762782A (zh) * 2019-02-27 2019-05-17 嘉文丽（福建）化妆品有限公司 一种促间充质干细胞生长的培养基及其制备方法
CN110317779A (zh) * 2019-06-06 2019-10-11 深圳市艾一生命科技有限公司 无血清的脐带间充质干细胞培养基
CN111518764A (zh) * 2020-06-04 2020-08-11 广州同康生物科技有限公司 一种脂肪干细胞无血清培养基及其制备方法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1863906A (zh) * 2000-11-22 2006-11-15 杰龙公司 多功能干细胞的耐受性同种异体移植物
US20070122906A1 (en) * 2003-12-29 2007-05-31 Allan Mishra Method of culturing cells
US20090202496A1 (en) * 2005-07-28 2009-08-13 Eden Biotech Ltd. Method for regenerating an immune system
WO2008100083A1 (en) * 2007-02-14 2008-08-21 Prostemics Co., Ltd. A composition of the injectable agents for tissues repaire containing mesenchymal stem cells and optimized culture media
CN109762782A (zh) * 2019-02-27 2019-05-17 嘉文丽（福建）化妆品有限公司 一种促间充质干细胞生长的培养基及其制备方法
CN110317779A (zh) * 2019-06-06 2019-10-11 深圳市艾一生命科技有限公司 无血清的脐带间充质干细胞培养基
CN111518764A (zh) * 2020-06-04 2020-08-11 广州同康生物科技有限公司 一种脂肪干细胞无血清培养基及其制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王海等: "骨间充质干细胞的研究现状", 《生物骨科材料与临床研究》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114107193A (zh) * 2021-12-14 2022-03-01 中国人民解放军联勤保障部队第九二〇医院 一种人脐带间充质干细胞的无分化扩增抗衰老培养基

Similar Documents

Publication Publication Date Title
CN110923196B (zh) 无血清培养基及其制备方法和间充质干细胞的培养方法
CN109762782B (zh) 一种促间充质干细胞生长的培养基及其制备方法
CN107267462B (zh) 一种诱导多能干细胞快速产生的无血清培养基
CN113736729B (zh) 组合物及含有其的干细胞无血清培养基及干细胞培养方法
CN104877962A (zh) 一种无血清的脂肪干细胞培养基及其制备方法
CN114480273A (zh) 用于获得间充质干细胞及其外泌体的培养基及其制备方法
CN113564111B (zh) 一种低氧培养脐带来源间充质干细胞的方法
CN114540298A (zh) 一种干细胞无血清培养基及其制备方法
CN110699317A (zh) 一种人脐带间充质干细胞无血清培养基及其制备方法与应用
CN111440764B (zh) 间充质干细胞的无血清培养基及间充质干细胞的临床级规模化培养方法
CN111235101B (zh) 一种人脐带间充质干细胞培养基及人脐带间充质干细胞的培养方法
CN112391340A (zh) 一种间充质干细胞培养基
CN111996164A (zh) 一种间充质干细胞的无血清抗衰老培养基
CN113249314B (zh) 促进间充质干细胞增殖与分化的培养方法及无血清培养基
CN113151165B (zh) 一种人脐带间充质干细胞扩增用培养基及培养方法
EP0248656B1 (en) Composition for cell cultivation and use thereof
CN112210532A (zh) 无血清培养基及其在间充质干细胞传代培养中的应用
CN107261132B (zh) 利用生物反应器生产猪伪狂犬病活疫苗的方法及其制品
CN106834213B (zh) 一种诱导多能干细胞形成毛乳头细胞的诱导培养基及诱导方法
CN114807028B (zh) 一种无血清间充质干细胞培养基及干细胞培养方法
CN117448268B (zh) 牙髓间充质干细胞的无血清培养基及其培养方法
CN111304163A (zh) 脐带间充质干细胞的规模化培养方法
CN111808799B (zh) 一种多功能干细胞培养方法及所用培养基
CN111004777B (zh) 一种间充质干细胞培养的补料及培养方法
CN113234672B (zh) 一种人间充质干细胞培养基及其应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20201127