CN111978365B - Preparation method of mogroside V-containing mogroside - Google Patents
Preparation method of mogroside V-containing mogroside Download PDFInfo
- Publication number
- CN111978365B CN111978365B CN202010944690.1A CN202010944690A CN111978365B CN 111978365 B CN111978365 B CN 111978365B CN 202010944690 A CN202010944690 A CN 202010944690A CN 111978365 B CN111978365 B CN 111978365B
- Authority
- CN
- China
- Prior art keywords
- mogroside
- liquid
- extraction
- membrane
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Abstract
The invention provides a preparation method of mogroside V-containing mogroside, which is used for enriching mogroside and glycoside V only by combining solvent extraction with decoloration and membrane filtration without depending on macroporous resin adsorption columns and other chromatographic techniques, and the content of mogroside V in the product is 50-70%. The quality index of the product can be compared favorably with the products of macroporous resin and chromatographic technique. The preparation method meets the standards of food and medicine safety and the like. The preparation process is energy-saving and environment-friendly, is simple and convenient to operate, has superior product quality, and has a large-scale industrial production prospect.
Description
Technical Field
The invention relates to food and health care products, in particular to a plant extract used as both medicine and food, and especially relates to a preparation method of environment-friendly mogroside V-containing mogroside.
Background
Lo Han Guo (Siraitia grosvenorii), a perennial vine of the Cucurbitaceae family. The traditional medicine uses the momordica grosvenori to have the effects of clearing heat and relieving cough, reducing phlegm, moistening lung, promoting the production of body fluid to quench thirst, lubricating intestines and expelling toxin and the like, and the momordica grosvenori is taken as a common traditional Chinese medicine in 1977 of Chinese pharmacopoeia and is a famous and precious traditional Chinese medicine with homology of medicine and food which is first published by the ministry of health of China. The momordica grosvenori component is separated and identified, and found to contain various fatty acids, amino acids, flavonoid compounds and a large amount of triterpenoid glycoside compounds. Mogrosides are a series of triterpenoid saponin compounds in momordica grosvenori, and are non-sugar substances with strong sweetness, and the sweetness of the mogrosides is about 300 times that of sucrose with the same quantity. Modern pharmacological studies prove that the mogroside also has various biological activities of blood sugar balance, fat metabolism, oxidation resistance, immunity improvement and the like, so the mogroside has great application prospects in the aspects of serving as food additives, functional foods, medicine-food dual-purpose traditional Chinese medicines and the like.
Mogroside, a novel low-calorie sugar-substituting sweetener, has been widely used in the production of foods, food additives and health products. In the 90 s of the 20 th century, the FDA in the united states approved mogroside as a sweetener to be added to food, and china approved mogroside as a sugar substitute for diabetics in 1996, allowing use in health products for flavoring instead of sucrose. In recent years, mogrosides has been approved as a food additive in countries such as the uk, korea, japan, singapore, and the like.
In the traditional Chinese medicine, the momordica grosvenori is used as a guiding drug of the traditional Chinese medicine, and has the effects of moistening lung for arresting cough, cooling blood, and relaxing bowel. In recent years, more physicochemical activities of mogrosides are continuously proved, and the physiological activity of the grosvenor momordica fruit is utilized to develop foods with special effects of resisting diabetes, aging, constipation, lipid reduction, weight loss, caries prevention and the like, so that the application of the grosvenor momordica fruit is changed from traditional medical and edible dual-purpose foods into functional and healthy new foods. The grosvenor momordica fruit and the mogroside thereof can be expected to have wide market prospect in the future.
The preparation technology of mogroside is known from known patent documents and published papers, and almost all of them utilize the adsorption function of macroporous resin, and the general process basically takes the grosvenor momordica fruit as the raw material: water extraction, macroporous resin adsorption and ethanol elution and resin decoloration; dilute ethanol extraction, macroporous resin adsorption and ethanol elution and resin decoloration; methanol extraction, macroporous resin adsorption and silica gel chromatography; squeezing, membrane filtration and macroporous resin adsorption; extracting with aqueous alcohol, concentrating, membrane filtering, macroporous resin-polyamide or silica gel chromatography-acetone recrystallization, etc. In the preparation, the extraction solution is obtained by almost extracting the momordica grosvenori glycoside and glycoside V with water, ethanol with water or methanol as solvent to obtain higher transfer rate, and macroporous resin adsorption and chromatography are needed for further increasing the content of the momordica grosvenori glycoside. Although the effect of enriching mogroside by using macroporous resin is good, the macroporous resin adsorption technology has the defect of extremely large waste water amount. The sewage treatment will prevent the growth and development of the fructus momordicae industry. Therefore, the method for preparing the momordica grosvenori extract containing the momordica grosvenori glycosides by using the non-macroporous resin adsorption technology has important significance.
Disclosure of Invention
The invention aims to overcome the defects, and develops a brand-new simple, environment-friendly and energy-saving preparation method of mogroside V according to the physicochemical properties of the mogroside compound and other compounds contained in the momordica grosvenori.
The invention provides a preparation method of mogroside V-containing mogroside.
The method of the invention comprises the following steps:
(1) raw material treatment: pulverizing fructus Siraitiae Grosvenorii into coarse powder, and further pulverizing with colloid mill or high-pressure homogenizer with pressure up to 100 MPa;
(2) extraction: adding a mixed solvent which is 10-15 times of the weight of the momordica grosvenori powder into the momordica grosvenori powder according to the weight ratio, extracting for 3 times, and obtaining momordica grosvenori extract after 1-2 hours each time;
(3) concentrating the extracting solution: concentrating the extractive solution to organic solvent-free solution by normal temperature or vacuum concentration;
(4) the concentrated solution is diluted by pure water, and the water consumption is 90-110% of the weight of the raw material of the momordica grosvenori. Filtering after dilution, and reserving filtrate for later use;
(5) refining: refining the filtrate by decolorizing with decolorizing resin and membrane filtering;
(6) concentrating the membrane filtrate: concentrating the refined filtrate in vacuum;
(7) drying to obtain mogroside V-containing mogroside.
The plant material used by the method is fresh fruits of momordica grosvenori (Siraitia grosvenor) which is a medicine and food dual-purpose plant issued by Ministry of health of the people's republic of China or momordica grosvenori fruits which are dried at low temperature and treated or stored in other forms containing momordica grosvenori sweet glycosides.
Said step (1) of the process of the present invention
Pulverizing with a pulverizer (model: L22-Y82 Hiuyang high-speed wall breaking conditioner) or (model: DCJ-100 double-roller crusher) to more than 100 meshes, and pulverizing with a colloid mill (model: JM100AL) with cooling in explosion-proof environment and a high-pressure homogenizer (model: SLS-60-70) with cooling in explosion-proof environment, or pulverizing with ball mill.
The solvent in the step (2) of the method is prepared by mixing ethyl acetate-ethanol or ethyl acetate-methanol with water. The weight ratio of the ethyl acetate to the raw materials is 10-13 times; the weight ratio of the ethanol or the methanol to the raw material is 1-2 times; the weight ratio of the water to the raw material is 0-1 time.
The extraction method can be heating reflux, heating stirring reflux, heating external circulation reflux and heating external circulation stirring reflux. Or extracting techniques such as heat percolation and continuous countercurrent extraction. Reflux extraction is carried out for 3 times and each time lasts for 1-2 hours.
Said step (3) of the method of the present invention
Concentration is preferably performed by steam heating and normal pressure concentration in an explosion-proof environment or by double-effect concentration, triple-effect concentration, vacuum concentration, external circulation concentration or other concentration technologies in the explosion-proof environment.
The filtration in the step (4) of the method of the invention is preferably high-speed centrifugal clarification, and can also use filtration technologies such as flat plate vacuum filtration, plate-and-frame filter pressing, membrane fine filtration and the like.
The step (5) of the method of the invention is refining and decoloring: preferably, a combination of weak anion exchange resins and strong cation exchange resins is used. The weak anion exchange resin is of the type: LX-363 or D-90, LX-67 or D301, without excluding the use of strong anion exchange resins. The types of strong cation exchange resins used were: 732. d072 or JK001 also does not exclude the use of weak cation exchange resins.
The membrane filtration refining in the step (6) adopts a pressure-driven membrane filtration technology, a membrane with the aperture of the filter membrane being the preferred molecular weight cut-off of 800-1200 daltons is selected, the membrane material form is the preferred roll type membrane, and can also be a flat membrane, a low-pressure fiber membrane or a ceramic membrane, and the molecular weight cut-off of the selected filter membrane is not limited to 800-1200 daltons.
The prepared product is a light brown yellow solid, wherein the content of mogroside V is 50-70%. In view of the great correlation between the color and the drying manner, the product prepared by the method is not limited to light brown yellow solid, and the product prepared by the method disclosed by the invention can also be light yellow solid or light brown solid.
The method disclosed by the invention is mainly characterized in that the enrichment of mogroside and glycoside V is realized only by combining solvent extraction with decoloration and membrane filtration without depending on macroporous resin adsorption columns and other chromatographic techniques, the content of the glycoside V in the product reaches over 50 percent, and the content of the mogroside V in the product can be comparable to that of the product prepared by adopting macroporous resin and chromatographic techniques. The preparation method meets the standards of food and medicine safety and the like. The preparation process is energy-saving and environment-friendly, has superior product quality, and has a large-scale industrial production prospect.
Detailed Description
The reverse osmosis drinking purified water used in the embodiment of the invention is prepared by a reverse osmosis drinking purified water machine with the equipment model number of KYRO025TH-1, the equipment meets the (Chinese national industry standard) HY/T068-2002 'SRO series reverse osmosis equipment of drinking water preparation system' standard, and the water quality meets the requirements specified in (Chinese national standard) GB17324-2003 'bottle (barrel) drinking purified water sanitary standard'.
Example 1.
0.5kg of fresh momordica grosvenori fruits are taken and firstly crushed into coarse powder by a common household high-speed food crusher (model: L22-Y82 Jiuyang high-speed wall breaking conditioning machine), and then mixed extraction solvent with the weight 12 times of that of the raw materials is added, wherein the first extraction solvent ratio is as follows: 5.25kg of ethyl acetate (10.5 times of the weight of the raw material), 0.75kg of 95% ethanol (1.5 times of the weight of the raw material) and 0kg of pure water, 6kg in total. Transferring the material to a material barrel of a high-pressure homogenizer with the model of SLS-60-70 to start the homogenizer to perform material homogenization operation under the conditions of explosion-proof environment and opening cooling water (15-20 ℃). The operation pressure is gradually increased from 0MPa to 40MPa, the time is consumed for 0.5h, then the circulation is maintained for 1h, the homogenization is fully carried out, and the material temperature is controlled between 40 ℃ and 50 ℃. The homogenized material is put into a 10L glass reactor, stirred and heated to boiling, then refluxed for 1h, stopped, and poured out to extract the material, and centrifuged by a centrifuge to obtain clear liquid. Centrifugation conditions: room temperature, rotation speed 4000 r/min. Digging out the centrifugal precipitate for subsequent extraction, wherein the solvent ratios of the second extraction and the third extraction are as follows: 5.25kg of ethyl acetate, 0.75kg of 95% ethanol and 0.375kg of pure water (0.75 times of the weight of the raw materials). This was done in triplicate. Combining the three centrifugated liquid, vacuum concentrating and recovering organic solvent to obtain concentrate, and adding water and vacuum distilling to remove residual organic solvent. The concentrate from which the organic solvent was removed was dissolved in warm water and diluted to 0.5L, and vacuum-filtered through a plate filter having a membrane pore size of 1 μ, and the filtrate was collected and the solution was decolorized by passing through a treated D-301 resin and a 732 cation exchange resin column in this order, and the resin column was washed with pure water after passing through the liquid. Combining the decolorized solution and the resin flushing solution, and performing pressure-driven membrane filtration by using a roll-up membrane with the molecular weight cutoff of 1200 daltons under the membrane filtration conditions: the pressure is 1-3 MPa, and the temperature is 30-40 ℃. And (3) confirming the end point of membrane filtration: and (3) measuring by using a conductivity meter, stopping membrane filtration when the passing liquid reaches 50-100 mu S/cm, discharging the trapped liquid, washing residual liquid in the membrane by using pure water, collecting and combining, and concentrating the liquid in vacuum until the liquid is dry to obtain the preparation product.
The product is detected according to the [ content determination ] of mogroside V in terms of fructus momordicae in the Chinese pharmacopoeia of 2015 edition, the content of mogroside V is 64.3%.
Example 2.
1.5kg of low-temperature dried fruits of fresh momordica grosvenori fruits are taken, firstly, a common household high-speed food grinder (model: L22-Y82 Jiuyang high-speed wall breaking conditioning machine) is adopted to grind the fruits into coarse powder, mixed extraction solvents with the weight 15 times of the weight of the raw materials are added, and the proportion of the solvents is as follows: 18.3kg of ethyl acetate (12.2 times of the weight of the raw material), 2.8kg of 95% ethanol (1.87 times of the weight of the raw material) and 1.4kg of water (0.93 times of the weight of the raw material) were added to 22.5kg in total. And then transferring the material to a model JM100AL colloid mill material tank to start the colloid mill under the conditions of an explosion-proof environment and cooling water opening (15-20 ℃), and circulating the material in the colloid mill for 0.5 h. The operation pressure is 0.05-0.2 MPa, and the material temperature is controlled at 25-30 ℃. Transfer of the ground material to 0.5M3Stirring and heating the reflux extraction tank to boil, refluxing for 1 hour, discharging the extract, cooling to room temperature, and centrifuging in a sedimentation centrifuge of model PC-450 at 2000r/min to obtain clear liquid. The centrifugation sediment is dug out for subsequent extraction, and the extraction is carried out twice again under the same conditions as the first extraction. Combining the three centrifugations, vacuum concentrating and recovering the organic solvent to obtain the concentrate. The residual organic solvent in the concentrate can be removed by adding water and vacuum distilling. The concentrate from which the organic solvent was removed was dissolved in warm water and discharged, and the concentrate was washed with pure water and diluted to 5 kg. Then vacuum filtering with a flat filter with the membrane aperture of 1 mu, collecting filtrate, sequentially passing the filtrate through the treated LX-363 resin column for decolorization and the D-072 resin column, and washing the resin column with pure water after the feed liquid is completely passed through. Mixing the decolorized solution and resin rinse solution, and treating with a solution having a molecular weight cut-off of 1000The roll-up membrane of erton performs a pressure driven membrane filtration operation, with the membrane filtration conditions: the pressure is 1-3 MPa, and the temperature is 30-40 ℃. And (3) confirming the end point of membrane filtration: and (3) measuring by using a conductivity meter, stopping when nanofiltration passing liquid reaches 50-100 mu S/cm, discharging trapped liquid, washing residual liquid in the membrane by using pure water, and collecting and combining the residual liquid. And (4) carrying out vacuum concentration on the combined materials, and stopping concentrating until the solid content in the material liquid reaches 20-25%. Concentration conditions were as follows: the vacuum degree is 0.095-0.098 MPa, the temperature is 80-85 ℃, and the feed liquid is discharged after concentration. Spray drying the materials under the following conditions: the air inlet temperature is 120 ℃, the air outlet temperature is 90 ℃, and spray-dried powder is collected to be a preparation product.
The product is detected according to the [ content determination ] of mogroside V in terms of fructus momordicae in the Chinese pharmacopoeia of 2015 edition, the content of mogroside V is 51.6%.
Example 3.
3kg of fresh momordica grosvenori fruits which are crushed into coarse powder by an industrial crusher and dried at low temperature are added with mixed extraction solvents with the weight 12.5 times of that of the raw materials, and the proportion of each solvent is as follows: 31.5kg of ethyl acetate (10.5 times of the weight of the raw material), 4.5kg of methanol (1.5 times of the weight of the raw material) and 1.5kg of water (0.5 times of the weight of the raw material), and 37.5kg of the total. Transferring the material to a material tank of a model JM100AL colloid mill under the conditions of an explosion-proof environment and opening cooling water (15-20 ℃), and starting the colloid mill to circulate the material in the colloid mill for 1 h. The operation pressure is 0.05-0.2 MPa, and the operation temperature is room temperature. Fine ground material input 0.5M3Stirring and heating the reflux extraction tank to boil, refluxing for 1 hour, discharging the extract, cooling to room temperature, and centrifuging in a sedimentation centrifuge of model PC-450 at 2000r/min to obtain clear centrifugate. The centrifugation pellet was scooped out for subsequent extraction and repeated three times using the above extraction parameters. Combining the three centrifugated liquid, vacuum concentrating and recovering organic solvent to obtain concentrate, and adding water and vacuum distilling to remove residual organic solvent. Dissolving the concentrate with warm water, discharging, washing the concentrator with water, diluting to 6L, vacuum filtering with plate filter with membrane pore diameter of 1 μ, collecting filtrate, and decolorizing the solution with treated Bayonine decolorizing-1 resin column and JK001 resin sequentially. Combining decolorized feed liquid and resinWashing liquid, and performing pressure-driven membrane filtration operation by using a roll-type membrane with the molecular weight cutoff of 800 daltons, wherein the membrane filtration conditions are as follows: the pressure is 1-3 Mpa, and the temperature is 30-40 ℃. And (3) confirming the end point of membrane filtration: measuring with a conductivity meter, stopping membrane filtration when the filtrate reaches 50-60 μ S/cm, discharging the trapped fluid, washing the residual liquid in the membrane with pure water, collecting and mixing. And (4) carrying out vacuum concentration on the combined materials until the solid content in the material liquid reaches 20-25%. Concentration conditions were as follows: the vacuum degree is 0.095-0.098 MPa, the temperature is 80-85 ℃, and the feed liquid is discharged after concentration. The material was further spray dried under the following conditions: the air inlet temperature is 120 ℃, the air outlet temperature is 90 ℃, and spray-dried powder is collected to be a preparation product.
The product is detected according to the [ content determination ] of mogroside V in terms of fructus momordicae in the Chinese pharmacopoeia of 2015 edition, the content of mogroside V is 53.1%.
Example 4
Taking 4kg of fresh momordica grosvenori fruits which are crushed into coarse powder by an industrial crusher, adding a mixed extraction solvent which is 10 times of the weight of the raw materials, wherein the first solvent proportion is as follows: 35kg of ethyl acetate (8.75 times of the weight of the raw material), 5kg of ethanol (1.25 times of the weight of the raw material) and 0kg of water, and 40kg of the total. Transferring the material to a material tank of a model JM100AL colloid mill under the conditions of an explosion-proof environment and opening cooling water (15-20 ℃), and circulating the material in the colloid mill for 0.5 h. The operation pressure is 0.05-0.2 MPa, and the operation temperature is room temperature. Transferring the ground material to a high-pressure homogenizer material barrel, opening the homogenizer to perform material homogenization. The time for the operation pressure to gradually rise from 0MPa to 40MPa is 1h, then the circulation is maintained for 2h, the homogenization is carried out fully, and the operation temperature is controlled between 40 ℃ and 50 ℃. Transfer of homogenized material to 0.5M3Stirring and heating the reflux extraction tank to boil, refluxing for 1 hour, discharging the extract, cooling to room temperature, and centrifuging in a sedimentation centrifuge of model PC-450 at 2000r/min to obtain clear centrifugate. The centrifugation pellet was scooped out for subsequent extraction. The solvent added in the second reflux extraction and the third reflux extraction is: 35kg of ethyl acetate, 5kg of ethanol and 3kg of water (0.75 times of the weight of the raw materials), 43kg each time in total (11 times of the weight of the raw materials). Combining the three centrifugates, and recovering organic solvent in a vacuum concentration potObtaining a concentrate, and adding water and then distilling in vacuum to remove residual organic solvent in the concentrate. Dissolving the concentrate with warm water, discharging, washing the concentrating pan with water, mixing and diluting to obtain 10L of feed liquid, and centrifuging with GQ type tubular centrifuge to obtain clear liquid. Sequentially passing the centrifugate through treated D-900 resin and D113 resin, combining the passing liquid and resin flushing liquid, and performing pressure-driven membrane filtration operation by using a roll-type filter membrane with the molecular weight cutoff of 800 daltons, wherein the membrane filtration conditions are as follows: the pressure is 2-4 Mpa, and the temperature is 40-60 ℃. And (3) confirming the end point of membrane filtration: and (3) measuring by using a conductivity meter, stopping when nanofiltration passing liquid reaches 50-60 mu S/cm, discharging trapped liquid, washing residual liquid in the membrane by using pure water, and collecting and combining the residual liquid. And (4) carrying out vacuum concentration on the combined materials until the solid content in the material liquid reaches 20-25%. Concentration conditions were as follows: the vacuum degree is 0.095-0.098 MPa, the temperature is 80-85 ℃, and the feed liquid is discharged after concentration. Spray drying the materials under the following conditions: the air inlet temperature is 120 ℃, the air outlet temperature is 90 ℃, and spray-dried powder is collected to be a preparation product.
The product is detected according to the [ content determination ] of mogroside V in terms of fructus momordicae in the Chinese pharmacopoeia of 2015 edition, the content of mogroside V is 58.1%.
Claims (1)
1. A preparation method of mogroside V-containing mogroside is characterized in that,
the method comprises the following steps: 0.5kg of fresh momordica grosvenori fruits are taken and firstly crushed into coarse powder by a common household high-speed food crusher, and then mixed extraction solvent with the weight 12 times that of the raw materials is added, wherein the first extraction solvent is prepared from the following components in parts by weight: 5.25kg of ethyl acetate, 0.75kg of 95% ethanol and 0kg of pure water, 6kg in total; transferring the material to a material barrel of a high-pressure homogenizer at 15-20 ℃ in an explosion-proof environment and by introducing cooling water, and starting the homogenizer to perform material homogenization; the operation pressure is increased gradually from 0MPa to 40MPa, the time is consumed for 0.5h, then the circulation and full homogenization are maintained for 1h, the material temperature is controlled between 40 ℃ and 50 ℃, the homogenized material is put into a 10L glass reactor, stirred and heated to boiling, then the reflux is stopped for 1h, the extracted material is poured out, and a centrifuge is used for centrifugal treatment to obtain clear liquid; centrifugation conditions: room temperature, rotation speed 4000 r/min. Digging out the centrifugal precipitate for subsequent extraction, wherein the solvent ratios of the second extraction and the third extraction are as follows: ethyl acetate 5.25kg, 95% ethanol 0.75kg and pure water 0.375 kg; performing three-time extraction, combining three-time centrifugal clear liquids, performing vacuum concentration to recover an organic solvent to obtain a concentrate, adding water into the concentrate, performing vacuum distillation to remove the residual organic solvent, dissolving the concentrate from which the organic solvent is removed with warm water, diluting to 0.5L, performing vacuum filtration by using a flat filter with a membrane pore size of 1 mu, collecting filtrate, and decoloring the solution by sequentially passing through a treated D-301 resin column and a 732 cation exchange resin column, wherein the resin column is washed by pure water after liquid materials are completely passed through; combining the decolorized solution and the resin flushing solution, and performing pressure-driven membrane filtration by using a roll-up membrane with the molecular weight cutoff of 1200 daltons under the membrane filtration conditions: the pressure is 1-3 MPa, and the temperature is 30-40 ℃; and (3) confirming the end point of membrane filtration: and (3) measuring by using a conductivity meter, stopping membrane filtration when the passing liquid reaches 50-100 mu S/cm, discharging the trapped liquid, washing residual liquid in the membrane by using pure water, collecting and combining, and concentrating the liquid in vacuum until the liquid is dry to obtain the preparation product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010944690.1A CN111978365B (en) | 2020-09-10 | 2020-09-10 | Preparation method of mogroside V-containing mogroside |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010944690.1A CN111978365B (en) | 2020-09-10 | 2020-09-10 | Preparation method of mogroside V-containing mogroside |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111978365A CN111978365A (en) | 2020-11-24 |
| CN111978365B true CN111978365B (en) | 2022-04-12 |
Family
ID=73449983
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010944690.1A Active CN111978365B (en) | 2020-09-10 | 2020-09-10 | Preparation method of mogroside V-containing mogroside |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111978365B (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690573B (en) * | 2009-10-16 | 2012-01-25 | 长沙绿蔓生物科技有限公司 | Production method of fructus monordicae extract with over 60% of mogroside V content |
| CN103467557A (en) * | 2013-09-11 | 2013-12-25 | 南京泽朗农业发展有限公司 | Method for extracting fructus momordicae extract through supercritical fluid |
| CN104558088A (en) * | 2015-01-23 | 2015-04-29 | 江西海富生物工程有限公司 | Method for extracting mogroside V from momordica grosvenori |
| CN105218612B (en) * | 2015-09-30 | 2017-10-03 | 大闽食品（漳州）有限公司 | A kind of method of Momordica grosvenori mogroside V purity in raising mogroside |
| CN107936079B (en) * | 2017-11-28 | 2020-05-01 | 河南中大恒源生物科技股份有限公司 | Method for preparing high-purity mogroside V |
| CN109320576B (en) * | 2018-10-25 | 2020-09-08 | 晨光生物科技集团股份有限公司 | Production method of high-content mogroside V |
-
2020
- 2020-09-10 CN CN202010944690.1A patent/CN111978365B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN111978365A (en) | 2020-11-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2020048049A1 (en) | Method for preparing luo han guo sweetener composition from siraitia grosvenorii and use thereof | |
| CN103145869B (en) | A kind of preparation method of the extract containing momordica grosvenori polysaccharide | |
| CN105767397A (en) | Spleen-invigorating and stomach-harmonizing gingko tea | |
| CN101386636B (en) | Mogroside extraction method from grosvenor momordica | |
| CN101112458B (en) | Process for extracting effective component of sweet tea | |
| CN107362200B (en) | Method for extracting and separating alkaloid and flavone from mulberry leaves | |
| CN107898868B (en) | Method for synchronously separating and preparing lycium erythrophyll, lycium barbarum polysaccharide and lycium barbarum flavone from lycium barbarum | |
| CN108516997A (en) | A method of extracting Rubusoside from sweet tea | |
| CN107629105A (en) | A kind of method of purification of flavor momordica glycoside V | |
| CN101671294B (en) | Method for continuously extracting and separating 1-deoxynojirimycin (DNJ) and flavone from folium mori | |
| CN102408463A (en) | Extraction method for mogroside | |
| CN113854533B (en) | A method for preparing mogroside, mogrolic acid, fructus Siraitiae Grosvenorii concentrated juice and fructus Siraitiae Grosvenorii fructose from fresh fructus Siraitiae Grosvenorii | |
| CN110105458A (en) | The method that polysaccharide and PEARLITOL 25C are extracted in waste liquid is extracted from mogroside | |
| CN102675398B (en) | A kind of method extracting momordica grosvenori glycoside V and farnesol from Grosvenor Momordica | |
| CN103478838A (en) | Honeysuckle kudzuvine root beverage and preparation method thereof | |
| CN109674843A (en) | A kind of method for extraction and purification of dried fructus momordicae comprehensive utilization | |
| CN108409640A (en) | A kind of method of purification of arecaline | |
| CN100447206C (en) | Method of extracting and preparing yellow pigment of pagodatree flower | |
| CN108690099A (en) | A method of the extraction separation procyanidine from black fruit fructus lycii | |
| CN105693680B (en) | A kind of extracting method of indigo fruit anthocyanidin | |
| CN102675399A (en) | Method for extracting mogroside V and mannitol from corsvenor momordica fruit | |
| CN101716005A (en) | Honeysuckle compound beverage and preparation method thereof | |
| CN109369733B (en) | Method for simultaneously extracting multiple flavonoid compounds from tartary buckwheat leaves | |
| CN108516996B (en) | Method for extracting fructus momordicae prebiotics and method for simultaneously extracting mogroside V | |
| CN110521903A (en) | It is a kind of have effects that anti-haze clearing lung-heat Siraitia grosvenorii honey and its production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |