CN105218612B - A kind of method of Momordica grosvenori mogroside V purity in raising mogroside - Google Patents
A kind of method of Momordica grosvenori mogroside V purity in raising mogroside Download PDFInfo
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Abstract
The invention discloses a kind of method for improving Momordica grosvenori mogroside V purity in mogroside, using Momordica grosvenori fresh fruit or Momordica grosvenori dry fruit as raw material, by 1）Extraction, 2）Pre-concentration, 3）Enzymolysis, 4）Once filtering, 5）Resin adsorption, elution, 6）Concentration, secondary filter, 7）Resin column desalination, 8）Resin column decolouring, 9）Activated carbon further decolouring and 10）Sterilizing, drying steps are completed.The present invention is adsorbed by adsorption column to the main working substance in feed liquid, then with the working substance on elution adsorption column, eluent finally is gone into removal of impurities taste through desalination resin, depigmentation is removed through decolorizing resin, finally by charcoal absorption, product is obtained after further removing the removal of impurity, sterilizing-drying.Final products color of the present invention is snow-white, and free from extraneous odour, mouthfeel is fresh and sweet, and rear sweet tea is long, class white granulated sugar, and sugariness is 500 1000 times of granulated sugar, while heat is almost 0, be can be applied to in sugar product and slimming health food.
Description
Technical field
The present invention relates to Momordica grosvenori manufacture field, and in particular to one kind improves Momordica grosvenori mogroside V purity in mogroside
Method.
Background technology
Mogroside is also known as Momordica-Glycosides, extracts in Guangxi special product economic plants -- and Momordica grosvenori, its sugariness is sucrose
300 times, its heat is zero, with clearing heat and moistening lung antibechic, relax bowel the effect of, have to obesity, constipation, diabetes etc. anti-
Control effect.
Monomer glucoside V in Momordica-Glycosides is the main sweet ingredient of Momordica-Glycosides.At present, Momordica-Glycosides is extracted
Method have a lot, but the reason for because of itself raw material, it is undesirable to take off taste decolorizing effect in extraction process, the arhat extracted
Fruit powder pink colour is partially deep, and has offending sentimental taste, i.e. monomer glucoside V purity is not high, is not suitable for industrial sweetener requirement.
The content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art effectively to remove the not mature of mogroside there is provided a kind of
Taste, and remove color and luster, the method for improving Momordica grosvenori mogroside V purity in mogroside.
To achieve the above object, the present invention is adopted the following technical scheme that：
The method of Momordica grosvenori mogroside V purity, comprises the following steps in a kind of raising mogroside：
1) extract：Momordica grosvenori raw material is subjected to break process, then will it is broken after raw material and volumetric concentration be 30-35%'s
Ethanol is by weight 1：9-10 is well mixed, at 70-75 DEG C of temperature, and 45- is extracted with three-level list tank shell type extracting method
60min, filtering, is obtained containing Momordica grosvenori mogroside V（Mogroside V）Extract solution, and extract solution is cooled to 25-30 DEG C；
2) pre-concentration：Extract solution after above-mentioned cooling is concentrated into the 1/5-1/4 of extracting liquid volume, concentrate is obtained；
3) digest：Acid solution regulation concentrate pH to 4.0-4.5 is added in above-mentioned concentrate, Brewers is then added
Compass complex enzymes, the complex enzyme addition is the 0.5-1 ‰ of concentrate weight, then heats to 50-55 DEG C, enzymolysis
30-40min；Turbidity is down to 1-2NTU from more than 4000NTU after enzymolysis, and monomer glucoside V concentration improves 10-15%, reduced simultaneously
Bitter taste.
4) once filter：By the concentrate centrifugation after above-mentioned enzymolysis, using 1-2um ceramic membrane filter, once filtered
Liquid；
5）Resin adsorption, elution：Macroporous absorbent resin is fitted into adsorption column, then by above-mentioned first-time filtrate with 2-3BV/
H flow velocity flows through adsorption column and adsorbed, and mainly adsorbs soaping agents, pigment and other taste compounds in first-time filtrate, often
Root adsorption column adsorbs the 40-50 times of first-time filtrate for adsorbing column volume, and absorption is washed after terminating with the eluent of 3-4 times of column volume of absorption
Saponin constituents, pigment and other taste compounds adsorbed on desorption attached column, the eluent is concentration 70-75% ethanol, is washed
Separation of flow speed is 1-2BV/h, and it is subsequently de- taste to collect the saponin constituents in eluent, the eluent（Desalination）What is decolourized is effective
Thing；
6）Concentration, secondary filter：The eluent of above-mentioned collection is concentrated into the concentrate that hundred profit degree are 25-30%, then will be dense
Contracting liquid obtains secondary filtrate through 1-2um ceramic membrane filters；
7）Resin column desalination：Use D280 macroreticular resins for desalination resin, be loaded into resin column, by above-mentioned secondary filter
Liquid flows through resin column with 1.5-2BV/h flow velocity, and collects efflux, is then that eluent enters with concentration 70-75 % ethanol
Row elutes and collects eluent, and eluent consumption is 2.0-2.2 times of D280 macroreticular resin volumes, merges efflux and elution
Liquid, gained desalinization liquor electrical conductivity is 1-5us/cm；
8）Resin column decolourizes：Use D392 macroreticular resins for decolorizing resin, be loaded into resin column, after above-mentioned merging
Efflux and eluent resin column is flowed through with 1.5-2BV/h flow velocity, progress decolourizes to obtain destainer, and gained destainer is light
It is yellow to colourless；
9）Activated carbon decolorizing：Its weight 5-8% activated carbon is added in above-mentioned destainer, is decolourized at 45-50 DEG C of temperature
50-60min, is then centrifuged for, and abandons precipitation, collects centrifugate, and gained centrifugate is colourless；
10）Sterilizing：By the sterilizing of above-mentioned centrifugate, dry, obtain the V of Mogroside containing high-purity mogroside.
Further, the step 1）In Momordica grosvenori raw material be ripe fresh fructus momordicae or dried fructus momordicae.
The moisture of the dried fructus momordicae is less than 10%.
The preparation method of the dried fructus momordicae is as follows：Fresh Momordica grosvenori is toasted into 12-14 h at 45-50 DEG C of temperature,
Then heat to 65-70 DEG C, toast 22-24h, then be cooled to 50-55 DEG C, be baked to moisture less than 10%, obtain dry arhat
Really.
Further, step 2）With step 6）Described in concentration be concentrated in vacuo.
The step 3）In acid solution for concentration 2-2.5% citric acid solution or 1mol/L hydrochloric acid solution.
The step 4）With step 9）Described in the rotating speed that centrifuges be 6500-7000r/min.
The step 5) in macroporous absorbent resin be HPD750 macroporous absorbent resins, it is Momordica grosvenori raw material that it, which fills post amount,
1/10-1/9。
The step 7）D280 macroreticular resins dress post amount be Momordica grosvenori raw material 1/40-1/38, the step 8）'s
The dress post amount of D392 macroreticular resins is the 1/40-1/38 of Momordica grosvenori raw material.
The step 10）Middle sterilizing refers to the 30-35s that sterilized at 134-138 DEG C of temperature, and the drying is spray drying.
Step 1 of the present invention）By low-concentration ethanol leaching mode, Momordica grosvenori monomer glucoside V leaching is ensure that, and can drop
The dissolution of low other impurities, reduction later separation purifying difficulty.Subsequently typically can be by sieve of product by an adsorbing separation
Chinese fruit monomer glucoside V content is improved to more than 45%, and 20% or so is improved than existing extraction process by water.
Step 2 of the present invention）Pass through cryogenic vacuum pre-concentration, it is to avoid the active ingredient in destruction feed liquid.Meanwhile, pre-concentration is extremely
Finite concentration carries out subsequent adsorbtion purifying again, can be by the time required to traditional adsorption and purification（48h or so）10h or so is foreshortened to, is carried
High workload efficiency and material does not go bad.
Step 3 of the present invention）The Brewers Compass complex enzymes are bought to DSM N. V.（DSM company）,
Brewers Compass complex enzymes mainly contain cellulase, pectase, acid protease and 1,4 beta-glucanase etc., pectase
And protease mainly plays clarification, reduction viscosity and improves local flavor, cellulase and 1,4 beta-glucanase can improve active principle
Dissolution, the present invention can remove pectin and other macromolecular stickums, such as protein in material, and polypeptide by enzymolysis
Deng bitter substance, upper column pressure during post absorption was reduced, and reduce impurity component.The enzymolysis defecation method of the present invention, with tradition
Flocculant defecation method compare, the loss to Momordica grosvenori monomer glucoside V is almost 0, and clarifying effect is efficiently rapid, general 30min
Left and right can complete clarification, and can improve Momordica grosvenori monomer glucoside V yield 10-15% or so.By enzymolysis process, it can solve
The problem of sweet tea glycosides is muddy after occurring in traditional handicraft.
Step 5 of the present invention）Adsorption column after elution, successively using 5%NaOH aqueous cleaning, 5% salt
Recycle and reuse can be achieved in aqueous acid cleaning.Step 7）Desalination（De- taste）Resin is realized using 5% aqueous hydrochloric acid solution cleaning
Recycle and reuse.Step 8）After decolorizing resin flows through absorption through eluent, successively using saturation NaCL solution elution chromoresin,
5%NaOH aqueous cleaning is realized and recycled.
The present invention is using the technical scheme of the above, and during feed liquid flows through adsorption column, adsorption column is to main in feed liquid
Working substance is adsorbed, while adsorbing the taste compound in material, then with the working substance on elution adsorption column, finally will
Eluent is through desalination（De- taste）Resin adsorption taste compound, through decolorizing resin depigmentation is removed, finally by charcoal absorption, enter one
Step goes the removal of impurity.Because taste compound main in Momordica grosvenori extract solution is salt, polypeptide, polyphenols, major impurity is
Pigment constituents, by the inventive method, mogroside V contents are more than 60% in last obtained product, and Total saponin is more than
90%, product color is snow-white, and Determination of Polyphenols is less than 1%, accordingly, it is capable to be effectively improved products taste, increases substance content.
The present invention farthest remains the active ingredient of material, carried while unpleasant taste and pigment is removed
The high outward appearance and mouthfeel of product, product colour is snow-white, and free from extraneous odour, mouthfeel is fresh and sweet, and rear sweet tea is long, and class white granulated sugar, sugariness is sand
500-1000 times of sugar, while heat is almost 0, can be applied to in sugar product and slimming health food.
Embodiment
The method of Momordica grosvenori mogroside V purity, comprises the following steps in a kind of raising mogroside：
1) extract：Momordica grosvenori raw material is subjected to break process, then will it is broken after raw material and volumetric concentration be 30-35%'s
Ethanol is by weight 1：9-10 is well mixed, at 70-75 DEG C of temperature, and 45- is extracted with three-level list tank shell type extracting method
60min, filtering, obtains the extract solution containing Mogroside V, and extract solution is cooled into 25-30 DEG C；
2) pre-concentration：Extract solution after above-mentioned cooling is concentrated into the 1/ of extracting liquid volume by single-effect vacuum evaporator
5-1/4, obtains concentrate；
3) digest：Acid solution regulation concentrate pH to 4.0-4.5 is added in above-mentioned concentrate, Brewers is then added
Compass complex enzymes, the complex enzyme addition is the 0.5-1 ‰ of concentrate weight, is warming up to 50-55 DEG C, digests 30-
40min；
4) once filter：Concentrate after above-mentioned enzymolysis is centrifuged under 6500-7000r/min rotating speed, 1- is then used
2um ceramic membrane filter, obtains first-time filtrate；
5）Resin adsorption, elution：HPD750 macroporous absorbent resins are fitted into adsorption column, then by above-mentioned first-time filtrate with
2-3BV/h flow velocity flows through adsorption column and adsorbed, soaping agents, pigment and other flavour volatiles in primary attachment first-time filtrate
Matter, every adsorption column adsorbs the 40-50 times of first-time filtrate for adsorbing column volume, and the dense of 3-4 times of column volume of absorption is used in absorption after terminating
Degree 70-75% ethanol is saponin constituents, partial pigment and other taste compounds for being adsorbed on elution adsorption column, is washed
Separation of flow speed is 1-2BV/h, collects eluent；
6）Concentration, secondary filter：Above-mentioned eluent is concentrated by single-effect vacuum evaporator, obtaining hundred profit degree is
25-30% concentrate, then by concentrate through 1-2um ceramic membrane filters, obtain secondary filtrate；
7）Resin column desalination：Use D280 macroreticular resins for desalination resin, be loaded into resin column, by above-mentioned secondary filter
Liquid flows through resin column with 1.5-2BV/h flow velocity, and collects efflux, is then that eluent enters with concentration 70-75 % ethanol
Row elutes and collects eluent, and eluent consumption is 2.0-2.2 times of D280 macroreticular resin volumes, merges efflux and elution
Liquid；
8）Resin column decolourizes：Use D392 macroreticular resins for decolorizing resin, be loaded into resin column, after above-mentioned merging
Efflux and eluent resin column is flowed through with 1.5-2BV/h flow velocity, progress decolourizes to obtain destainer, and gained destainer is light
It is yellow to colourless；
9）Activated carbon decolorizing：Its weight 5-8% activated carbon is added in above-mentioned destainer, is decolourized at 45-50 DEG C of temperature
50-60min, is then centrifuged under 6500-7000r/min rotating speed, abandons precipitation, collects centrifugate, and gained centrifugate is nothing
Color；
10）Sterilize, dry：By above-mentioned centrifugate at 134-138 DEG C of temperature sterilizing 30-35s and then dry by spraying
It is dry, obtain the V of Mogroside containing high-purity mogroside.
Further.Step 1）Described Momordica grosvenori raw material is less than 10% dry sieve for ripe fresh fructus momordicae or moisture
Chinese fruit.
The preparation method of the dried fructus momordicae is as follows：Fresh Momordica grosvenori is toasted into 12-14 h at 45-50 DEG C of temperature,
Then heat to 65-70 DEG C, toast 22-24h, then be cooled to 50-55 DEG C, be baked to moisture less than 10%, obtain dry arhat
Really.
Step 3）The acid solution is concentration 2-2.5% citric acid solution or concentration 1mol/L hydrochloric acid solution.
Step 5) the dress post amounts of the HPD750 macroporous absorbent resins is the 1/10-1/9 of Momordica grosvenori raw material.
Step 7）The dress post amount of the D280 macroreticular resins is the 1/40-1/38 of Momordica grosvenori raw material.
Step 8）The dress post amount of the D392 macroreticular resins is the 1/40-1/38 of Momordica grosvenori raw material.
Embodiment 1
The method of Momordica grosvenori mogroside V purity, comprises the following steps in a kind of raising mogroside：
1) extract：By Momordica grosvenori raw material（Ripe fresh fructus momordicae or dried fructus momordicae）Carry out break process, then will it is broken after
Raw material and volumetric concentration for 30% ethanol by weight 1：10 are well mixed, and under temperature 70 C, are carried with three-level list tank shell type
Method is taken to extract 45min, filtering obtains the extract solution containing monomer glucoside V, and extract solution is cooled into 25 DEG C；
2) pre-concentration：Extract solution after above-mentioned cooling is concentrated into the 1/ of extracting liquid volume by single-effect vacuum evaporator
5, obtain concentrate；
3) digest：The citric acid solution of concentration 2% is added in above-mentioned concentrate, concentrate pH to 4.0, Ran Houjia is adjusted
Enter Brewers Compass complex enzymes, the complex enzyme addition is the 0.6 ‰ of concentrate weight, is warming up to 55 DEG C, enzyme
Solve 30min；
4) once filter：Concentrate after above-mentioned enzymolysis is centrifuged under 6500r/min rotating speed, then with 1um pottery
Porcelain membrane filtration, obtains first-time filtrate；
5）Resin adsorption, elution：HPD750 macroporous absorbent resins are fitted into adsorption column（It is Momordica grosvenori raw material to fill post amount
1/10）, above-mentioned first-time filtrate is then flowed through into adsorption column with 2BV/h flow velocity and adsorbed, in primary attachment first-time filtrate
Soaping agents, pigment and other taste compounds, every adsorption column adsorb the first-time filtrate of 40 times of absorption column volumes, and absorption is used after terminating
70% ethanol of 4 times of column volume of absorption is the saponin constituents adsorbed on elution adsorption column, partial pigment and other be in
Taste material, elution flow rate is 1BV/h, collects eluent；
6）Concentration, secondary filter：Above-mentioned eluent is concentrated by single-effect vacuum evaporator, obtaining hundred profit degree is
25% concentrate, then by concentrate through 1um ceramic membrane filters, obtain secondary filtrate；
7）Resin column desalination：Use D280 macroreticular resins for desalination resin, be loaded into resin column（It is arhat to fill post amount
The 1/40 of fruit raw material）, above-mentioned secondary filtrate is flowed through into resin column with 2BV/h flow velocity, and efflux is collected, then use concentration
70-% ethanol is that eluent is eluted and collects eluent, and eluent consumption is 2.0 times of D280 macroreticular resin volumes, is closed
And efflux and eluent；
8）Resin column decolourizes：Use D392 macroreticular resins for decolorizing resin, be loaded into resin column（It is arhat to fill post amount
The 1/40 of fruit raw material）, the efflux and eluent after above-mentioned merging are flowed through into resin column with 2BV/h flow velocity, progress is decolourized
To destainer, gained destainer is yellowish to colourless；
9）Activated carbon decolorizing：The activated carbon of its weight 5% is added in above-mentioned destainer, is decolourized under temperature 45 C
60min, is then centrifuged under 6500r/min rotating speed, abandons precipitation, collects centrifugate, and gained centrifugate is colourless；
10）Sterilize, dry：By above-mentioned centrifugate at 136 DEG C of temperature sterilizing 30s and then by spray drying, contained
High-purity Mogroside V mogroside.
Mogroside color and luster manufactured in the present embodiment is snow-white, and free from extraneous odour, mouthfeel is fresh and sweet, and rear sweet tea is long, wherein
Mogroside V contents 63%, total saponin content 91%, Determination of Polyphenols is less than 1%.
Embodiment 2
The method of Momordica grosvenori mogroside V purity, comprises the following steps in a kind of raising mogroside：
1) extract：By Momordica grosvenori raw material（Ripe fresh fructus momordicae or dried fructus momordicae）Carry out break process, then will it is broken after
Raw material and volumetric concentration for 35% ethanol by weight 1：9 are well mixed, and at 75 DEG C of temperature, are carried with three-level list tank shell type
Method is taken to extract 60min, filtering obtains the extract solution containing Mogroside V, and extract solution is cooled into 30 DEG C；
2) pre-concentration：Extract solution after above-mentioned cooling is concentrated into the 1/ of extracting liquid volume by single-effect vacuum evaporator
4, obtain concentrate；
3) digest：The citric acid solution of concentration 2.5% is added in above-mentioned concentrate, concentrate pH to 4.5 is adjusted, then
Brewers Compass complex enzymes are added, the complex enzyme addition is the 0.5 ‰ of concentrate weight, is warming up to 50 DEG C,
Digest 40min；
4) once filter：Concentrate after above-mentioned enzymolysis is centrifuged under 7000r/min rotating speed, then with 2um pottery
Porcelain membrane filtration, obtains first-time filtrate；
5）Resin adsorption, elution：HPD750 macroporous absorbent resins are fitted into adsorption column（It is Momordica grosvenori raw material to fill post amount
1/9）, above-mentioned first-time filtrate is then flowed through into adsorption column with 3BV/h flow velocity and adsorbed, the soap in primary attachment first-time filtrate
Glucoside, pigment and other taste compounds, every adsorption column adsorb the first-time filtrate of 50 times of absorption column volumes, and absorption is used after terminating inhales
75% ethanol of 3 times of attached column volume is saponin constituents, partial pigment and other flavor adsorbed on elution adsorption column
Material, elution flow rate is 2BV/h, collects eluent；
6）Concentration, secondary filter：Above-mentioned eluent is concentrated by single-effect vacuum evaporator, obtaining hundred profit degree is
30% concentrate, then by concentrate through 2um ceramic membrane filters, obtain secondary filtrate；
7）Resin column desalination：Use D280 macroreticular resins for desalination resin, be loaded into resin column（It is arhat to fill post amount
The 1/38 of fruit raw material）, above-mentioned secondary filtrate is flowed through into resin column with 1.5BV/h flow velocity, and efflux is collected, then use concentration
75 % ethanol is that eluent is eluted and collects eluent, and eluent consumption is 2.2 times of D280 macroreticular resin volumes, is closed
And efflux and eluent；
8）Resin column decolourizes：Use D392 macroreticular resins for decolorizing resin, be loaded into resin column（It is arhat to fill post amount
The 1/38 of fruit raw material）, the efflux and eluent after above-mentioned merging are flowed through into resin column with 1.5BV/h flow velocity, decolourized
Destainer is obtained, gained destainer is yellowish to colourless；
9）Activated carbon decolorizing：The activated carbon of its weight 8% is added in above-mentioned destainer, is decolourized under temperature 50 C
50min, is then centrifuged under 7000r/min rotating speed, abandons precipitation, collects centrifugate, and gained centrifugate is colourless；
10）Sterilize, dry：By above-mentioned centrifugate at 134 DEG C of temperature sterilizing 35s and then by spray drying, contained
High-purity Mogroside V mogroside.
Mogroside color and luster manufactured in the present embodiment is snow-white, and free from extraneous odour, mouthfeel is fresh and sweet, and rear sweet tea is long, wherein
Mogroside V contents are 65%, and total saponin content 90%, Determination of Polyphenols is less than 1%.
Embodiment 3
The method of Momordica grosvenori mogroside V purity, comprises the following steps in a kind of raising mogroside：
1) extract：By Momordica grosvenori raw material（Ripe fresh fructus momordicae or dried fructus momordicae）Carry out break process, then will it is broken after
Raw material and volumetric concentration for 30% ethanol by weight 1：10 are well mixed, and under temperature 70 C, are carried with three-level list tank shell type
Method is taken to extract 60min, filtering obtains the extract solution containing Mogroside V, and extract solution is cooled into 25 DEG C；
2) pre-concentration：Extract solution after above-mentioned cooling is concentrated into the 1/ of extracting liquid volume by single-effect vacuum evaporator
4, obtain concentrate；
3) digest：Concentration 1mol/L hydrochloric acid solution is added in above-mentioned concentrate, concentrate pH to 4.0 is adjusted, then
Brewers Compass complex enzymes are added, the complex enzyme addition is the 1 ‰ of concentrate weight, is warming up to 55 DEG C, enzyme
Solve 30min；
4) once filter：Concentrate after above-mentioned enzymolysis is centrifuged under 7000r/min rotating speed, then with 1um pottery
Porcelain membrane filtration, obtains first-time filtrate；
5）Resin adsorption, elution：HPD750 macroporous absorbent resins are fitted into adsorption column（It is Momordica grosvenori raw material to fill post amount
1/10）, above-mentioned first-time filtrate is then flowed through into adsorption column with 2.5BV/h flow velocity and adsorbed, in primary attachment first-time filtrate
Soaping agents, pigment and other taste compounds, every adsorption column adsorbs the first-time filtrates of 45 times of absorption column volumes, after absorption terminates
With absorption 3.5 times of column volume 70% ethanol be adsorbed on elution adsorption column saponin constituents, partial pigment and its
His taste compound, elution flow rate is 1.5BV/h, collects eluent；
6）Concentration, secondary filter：Above-mentioned eluent is concentrated by single-effect vacuum evaporator, obtaining hundred profit degree is
25% concentrate, then by concentrate through 1um ceramic membrane filters, obtain secondary filtrate；
7）Resin column desalination：Use D280 macroreticular resins for desalination resin, be loaded into resin column（It is arhat to fill post amount
The 1/40 of fruit raw material）, above-mentioned secondary filtrate is flowed through into resin column with 2BV/h flow velocity, and efflux is collected, then with concentration 70
% ethanol is that eluent is eluted and collects eluent, and eluent consumption is 2.0 times of D280 macroreticular resin volumes, is merged
Efflux and eluent；
8）Resin column decolourizes：Use D392 macroreticular resins for decolorizing resin, be loaded into resin column（It is arhat to fill post amount
The 1/40 of fruit raw material）, the efflux and eluent after above-mentioned merging are flowed through into resin column with 2BV/h flow velocity, progress is decolourized
To destainer, gained destainer is yellowish to colourless；
9）Activated carbon decolorizing：The activated carbon of its weight 6% is added in above-mentioned destainer, is decolourized under temperature 45 C
60min, is then centrifuged under 7000r/min rotating speed, abandons precipitation, collects centrifugate, and gained centrifugate is colourless；
10）Sterilize, dry：By above-mentioned centrifugate at 138 DEG C of temperature sterilizing 30s and then by spray drying, contained
High-purity Mogroside V mogroside.
Mogroside color and luster manufactured in the present embodiment is snow-white, and free from extraneous odour, mouthfeel is fresh and sweet, and rear sweet tea is long, wherein
Mogroside V contents 62%, total saponin content 91%, Determination of Polyphenols is less than 1%.
Embodiment 4
The method of Momordica grosvenori mogroside V purity, comprises the following steps in a kind of raising mogroside：
1) extract：By Momordica grosvenori raw material（Ripe fresh fructus momordicae or dried fructus momordicae）Carry out break process, then will it is broken after
Raw material and volumetric concentration for 30% ethanol by weight 1：10 are well mixed, and under temperature 70 C, are carried with three-level list tank shell type
Method is taken to extract 50min, filtering obtains the extract solution containing Mogroside V, and extract solution is cooled into 30 DEG C；
2) pre-concentration：Extract solution after above-mentioned cooling is concentrated into the 1/ of extracting liquid volume by single-effect vacuum evaporator
5, obtain concentrate；
3) digest：Concentration 1mol/L hydrochloric acid solution is added in above-mentioned concentrate, concentrate pH to 4.5 is adjusted, then
Brewers Compass complex enzymes are added, the complex enzyme addition is the 0.8 ‰ of concentrate weight, is warming up to 50 DEG C, enzyme
Solve 40min；
4) once filter：Concentrate after above-mentioned enzymolysis is centrifuged under 6500r/min rotating speed, then with 1um pottery
Porcelain membrane filtration, obtains first-time filtrate；
5）Resin adsorption, elution：HPD750 macroporous absorbent resins are fitted into adsorption column（It is Momordica grosvenori raw material to fill post amount
1/10）, above-mentioned first-time filtrate is then flowed through into adsorption column with 2BV/h flow velocity and adsorbed, in primary attachment first-time filtrate
Soaping agents, pigment and other taste compounds, every adsorption column adsorb the first-time filtrate of 0 times of absorption column volume, and absorption is used after terminating
70% ethanol of 3 times of column volume of absorption is the saponin constituents adsorbed on elution adsorption column, partial pigment and other be in
Taste material, elution flow rate is 1BV/h, collects eluent；
6）Concentration, secondary filter：Above-mentioned eluent is concentrated by single-effect vacuum evaporator, obtaining hundred profit degree is
30% concentrate, then by concentrate through 1um ceramic membrane filters, obtain secondary filtrate；
7）Resin column desalination：Use D280 macroreticular resins for desalination resin, be loaded into resin column（It is arhat to fill post amount
The 1/40 of fruit raw material）, above-mentioned secondary filtrate is flowed through into resin column with 2BV/h flow velocity, and efflux is collected, then with concentration 70
% ethanol is that eluent is eluted and collects eluent, and eluent consumption is 2 times of D280 macroreticular resin volumes, merges stream
Go out liquid and eluent；
8）Resin column decolourizes：Use D392 macroreticular resins for decolorizing resin, be loaded into resin column（It is arhat to fill post amount
The 1/40 of fruit raw material）, the efflux and eluent after above-mentioned merging are flowed through into resin column with 2BV/h flow velocity, progress is decolourized
To destainer, gained destainer is yellowish to colourless；
9）Activated carbon decolorizing：The activated carbon of its weight 5% is added in above-mentioned destainer, is decolourized under temperature 45 C
60min, is then centrifuged under 65000r/min rotating speed, abandons precipitation, collects centrifugate, and gained centrifugate is colourless；
10）Sterilize, dry：By above-mentioned centrifugate at 136 DEG C of temperature sterilizing 30s and then by spray drying, contained
High-purity Mogroside V mogroside.
Mogroside color and luster manufactured in the present embodiment is snow-white, and free from extraneous odour, mouthfeel is fresh and sweet, and rear sweet tea is long, wherein
Mogroside V contents 67%, total saponin content 90%, Determination of Polyphenols is less than 1%.
Embodiment 5
The preparation method of raw material dried fructus momordicae is as follows：Fresh Momordica grosvenori is toasted into 14 h, Ran Housheng under temperature 45 C
Temperature toasts 24h to 70 DEG C, then is cooled to 55 DEG C, is baked to moisture less than 10%, obtains dried fructus momordicae.
Embodiment 6
The preparation method of raw material dried fructus momordicae is as follows：Fresh Momordica grosvenori is toasted into 12 h, Ran Housheng under temperature 50 C
Temperature toasts 22h to 65 DEG C, then is cooled to 50 DEG C, is baked to moisture less than 10%, obtains dried fructus momordicae.
Claims (10)
1. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside, it is characterised in that：It comprises the following steps：
1) extract：Momordica grosvenori raw material is subjected to break process, then will it is broken after raw material and ethanol that volumetric concentration is 30-35%
By weight 1：9-10 is well mixed, at 70-75 DEG C of temperature, extracts 45-60min, and filtering is obtained containing Momordica grosvenori mogroside V
Extract solution, and extract solution is cooled to 25-30 DEG C；
2) pre-concentration：Extract solution after above-mentioned cooling is concentrated into the 1/5-1/4 of extracting liquid volume, concentrate is obtained；
3) digest：Acid solution regulation concentrate pH to 4.0-4.5 is added in above-mentioned concentrate, Brewers is then added
Compass complex enzymes, the addition of the complex enzyme is the 0.5-1 ‰ of concentrate weight, then heats to 50-55 DEG C, enzymolysis
30-40min；
4) once filter：By the concentrate centrifugation after above-mentioned enzymolysis, using 1-2um ceramic membrane filter, first-time filtrate is obtained；
5）Resin adsorption, elution：Macroporous absorbent resin is fitted into adsorption column, then by above-mentioned first-time filtrate with 2-3BV/h's
Flow velocity flows through adsorption column and adsorbed, and absorption 3-4 times of elution of column volume is used in absorption after terminating, and the eluent is concentration
70-75% ethanol, elution flow rate is 1-2BV/h, collects eluent；
6）Concentration, secondary filter：The eluent of above-mentioned collection is concentrated into the concentrate that hundred profit degree are 25-30%, then by concentrate
Through 1-2um ceramic membrane filters, secondary filtrate is obtained；
7）Resin column desalination：Use D280 macroreticular resins for desalination resin, be loaded into resin column, by above-mentioned secondary filtrate with
1.5-2BV/h flow velocity flows through resin column, and collects efflux, is then that eluent is washed with concentration 70-75 % ethanol
Take off and collect eluent, eluent consumption is 2.0-2.2 times of D280 macroreticular resin volumes, merges efflux and eluent；
8）Resin column decolourizes：Use D392 macroreticular resins for decolorizing resin, be loaded into resin column, by the stream after above-mentioned merging
Go out liquid and eluent and resin column is flowed through with 1.5-2BV/h flow velocity, progress decolourizes to obtain destainer；
9）Activated carbon decolorizing：Its weight 5-8% activated carbon is added in above-mentioned destainer, decolourize 50- at 45-50 DEG C of temperature
60min, is then centrifuged for, and abandons precipitation, collects centrifugate；
10）Sterilize, dry：By the sterilizing of above-mentioned centrifugate, dry, obtain the mogroside containing high-purity Momordica grosvenori mogroside V.
2. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 1, its feature exists
In：Step 1）Described Momordica grosvenori raw material is ripe fresh fructus momordicae or dried fructus momordicae.
3. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 2, its feature exists
In：The moisture of the dried fructus momordicae is less than 10%.
4. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 3, its feature exists
In：The preparation method of the dried fructus momordicae is as follows：By fresh Momordica grosvenori at 45-50 DEG C of temperature baking 12-14 h, Ran Housheng
Temperature toasts 22-24h to 65-70 DEG C, then is cooled to 50-55 DEG C, is baked to moisture less than 10%, obtains dried fructus momordicae.
5. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 1, its feature exists
In：Step 2）With step 6）Described in concentration be concentrated in vacuo.
6. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 1, its feature exists
In：Step 3）The acid solution is concentration 2-2.5% citric acid solution or 1mol/L hydrochloric acid solution.
7. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 1, its feature exists
In：Step 4）With step 9）Described in the rotating speed that centrifuges be 6500-7000r/min.
8. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 1, its feature exists
In：Step 5) macroporous absorbent resin is HPD750 macroporous absorbent resins, it fills the 1/10-1/9 that post amount is Momordica grosvenori raw material.
9. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 1, its feature exists
In：Step 7）The dress post amount of the D280 macroreticular resins is the 1/40-1/38 of Momordica grosvenori raw material, step 8）The D392 macropores tree
The dress post amount of fat is the 1/40-1/38 of Momordica grosvenori raw material.
10. a kind of method for improving Momordica grosvenori mogroside V purity in mogroside according to claim 1, its feature exists
In：Step 10）The sterilizing refers to the 30-35s that sterilized at 134-138 DEG C of temperature, and the drying is spray drying.
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| CN106008645B (en) * | 2016-06-06 | 2017-12-15 | 江西海富生物工程有限公司 | A kind of method that momordica grosvenori glycoside V is extracted from Momordica grosvenori |
| CN106418423A (en) * | 2016-09-27 | 2017-02-22 | 桂林益天成生物科技有限公司 | Preparation method of de-bitterized momordica grosvenori extract |
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| CN106432395A (en) * | 2016-09-27 | 2017-02-22 | 桂林益天成生物科技有限公司 | Extraction method of mogroside |
| CN106432394A (en) * | 2016-09-27 | 2017-02-22 | 桂林益天成生物科技有限公司 | Method for extracting siraitia grosvenorii total saponin in siraitia grosvenorii |
| CN106432396A (en) * | 2016-09-27 | 2017-02-22 | 桂林益天成生物科技有限公司 | Method for removing bitter substances of momordica grosvenori extract |
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| CN107602653B (en) * | 2017-10-09 | 2020-06-16 | 晨光生物科技集团股份有限公司 | Method for extracting mogroside from fresh momordica grosvenori |
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| CN109674843B (en) * | 2019-01-30 | 2022-03-22 | 湖南绿蔓生物科技股份有限公司 | Extraction and purification method for comprehensively utilizing dried momordica grosvenori |
| CN110521993A (en) * | 2019-08-13 | 2019-12-03 | 湖南艾达伦科技有限公司 | Momordia grosvenori aglycone sweet taste and flavour compositions and the preparation method and application thereof |
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| CN111978365B (en) * | 2020-09-10 | 2022-04-12 | 上海诺德生物实业有限公司 | Preparation method of mogroside V-containing mogroside |
| CN112825952A (en) * | 2020-12-31 | 2021-05-25 | 桂林实力科技有限公司 | Low-glycemic-index fructus momordicae fibrous candy and preparation method thereof |
| CN113068815B (en) * | 2021-04-14 | 2023-09-15 | 湖南华诚生物资源股份有限公司 | Momordica grosvenori fructose capable of improving sweetness and preparation method thereof |
| CN113142538B (en) * | 2021-04-16 | 2023-09-15 | 湖南华诚生物资源股份有限公司 | Zero-calorie candy for improving sweet taste performance of mogrosides and preparation method thereof |
| CN114166971B (en) * | 2021-12-03 | 2023-09-01 | 贵州茅台酒股份有限公司 | Detection method of cucurbitane-type triterpenoid saponin compound |
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