CN101327244A - Decolored and debitterized Momordica grosvenori extract and preparation method thereof - Google Patents
Decolored and debitterized Momordica grosvenori extract and preparation method thereof Download PDFInfo
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- CN101327244A CN101327244A CNA2008103032386A CN200810303238A CN101327244A CN 101327244 A CN101327244 A CN 101327244A CN A2008103032386 A CNA2008103032386 A CN A2008103032386A CN 200810303238 A CN200810303238 A CN 200810303238A CN 101327244 A CN101327244 A CN 101327244A
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Abstract
The invention discloses an extractive of discoloring, debitterizing corsvenor momordica fruit and a preparation method thereof, the method is as follows: the corsvenor momordica fruit is cleaned, crushed and extracted to juice, the fruit juice is centrifugated, the supernatant fluid enters into the macroporous absorption resin, the resin is washed by water, alkali solvent and water in order, till the effluent liquid is neutral; and then is eluted by 30 percent -65 percent ethanol, the ethanol eluent is collected, ethanol is recovered and dried, and then the extractive is obtained. The technology is simple, the production cost is low, and the production is easy to control; the traditional heating extracting method is replaced by extracting the juice, so the energy consumption is reduced, the color of the obtained extractive is light, the impurity is little, and the product quality is better; the adopted absorption resin achieves better discoloring, debitterizing effects at the same time of enriching the active components completely, the content of momordica glycosides is improved, the pigment and the impurity in the extractive are removed completely through eluting; the obtained product is white powder, the taste is sweet and pure, without traditional bitter taste, wherein, the gypenosides weight content of momordica glycosides can reach over 90 percent, and the V weight content of momordica glycosides can reach over 60 percent.
Description
(1) technical field:
The present invention relates to a kind of decolouring, debitterized Momordica grosvenori extract and preparation method thereof, belong to the extractive technique field of medicinal active ingredient.
(2) background technology:
Fructus Momordicae sweet in the mouth, acid, cool in nature, return lung, large intestine channel, effects such as clearing away heat and cooling blood, promoting the production of body fluid to quench thirst, loosening bowel to relieve constipation, skin care benefit face, moistening the lung and resolving phlegm, cough-relieving are arranged, cure mainly diseases such as pertussis, phlegm-fire cough, the dry constipation of blood, can treat obesity, diabetes, acute/chronic bronchitis, tonsillitis, pharyngolaryngitis, acute gastritis, asthma, hypertension and diabetes etc., and can be used for life prolonging, preserve youthful looks Yue Se and summer-heat impairment of body fluid, the agitation etc. of quenching one's thirst.The main component of Fructus Momordicae extract is a Momordica-Glycosides, comprise that mogroside IIE, III, IIIE, IV, V and VI, momordica grosvenori alcohol, Simon glycoside I, 1 foretell carbonyl mogroside V, the new glycoside of Fructus Momordicae, and mogroside V wherein is the Main Ingredients and Appearance of its sweet taste.Because its sugariness height, heat are low, pharmacology test shows Momordica-Glycosides has blood pressure lowering and hypolipidemic activity, have antiinflammatory again, ease pain, separate stem, fall the effect of enzyme, can be used for life prolonging, diseases such as preserve youthful looks Yue Se and treatment phlegm-heat cough, laryngopharynx swelling and pain, constipation, the agitation of quenching one's thirst.Relevant research shows that also the effective ingredient that Fructus Momordicae plays a major role to various diseases is exactly a Fructus Momordicae dulcoside V.Because Momordica-Glycosides has good health-care effect, to promote the intestines and stomach function and clearing and antitussive, quench the thirst reduce phlegm, the effect of laxation toxin expelling, skin care benefit face, clearing heat and moistening lung is more remarkable; Compare with artificial synthesis edulcorant and other sweeting agent, the Momordica-Glycosides edible safety, sugariness height (pure product sugariness is 300 times of sucrose) is best sweeting agent of patients such as obesity, hypertension, diabetes, heart disease and best health product.Therefore, Momordica-Glycosides can be widely used in the industries such as food, medicine, beverage, gerocomia product.
The preparation method of traditional Fructus Momordicae extract mainly contains two kinds: (1) water is put forward the resin isolation method: this technology is with the pressurized tank of packing into after the Fructus Momordicae fragmentation, add the water extraction of heating, repeatedly the water extract merges filtration, last resin carries out adsorbing separation, with ethanol or methanol-eluted fractions, after eluent removes alcohol, spray drying and getting; (2) alcohol extraction membrane separation process: its technology is with the Fructus Momordicae fragmentation, adds 50% ethanol, the room temperature or the lixiviate of heating in the storage tank of packing into, and extracted many times liquid merges filtration, and filtrate concentrates by membrance separation, and concentrated solution carries out vacuum drying and gets.The Fructus Momordicae extract color and luster that adopts these two kinds of methods to obtain be dark yellow to yellowish-brown, and have strong taste of Chinese medicine and intensive back bitterness.
CN101007042A discloses the preparation method of the Fructus Momordicae extract of a kind of decolouring, debitterize, with the Fructus Momordicae is raw material, use water boiling and extraction, extracting solution after filtration, concentrate, resin decolorization, ethanol elution, deodorization agent are taken off flavor, reclaim ethanol, concentrate, drying and getting.Though this method has obtained the extract product of decolouring, debitterize, its technology has still adopted the mode of heating extraction, and the color of extracting solution is darker, is unfavorable for subsequent decolorization, takes off flavor and handle; The resin decolorization that is adopted, to take off the flavor effect not ideal enough, must could collect effective ingredient with the ethanol elution of higher concentration, and ethanol elution must adsorb just and can achieve the goal once more with deodorization agent.
(3) summary of the invention:
Technical problem to be solved by this invention is: change the traditional heating extraction mode of Fructus Momordicae, Fructus Momordicae extract with improved process preparation decolouring, debitterize, can fully remove pigment and impurity in the extract, extract obtained of light color, the active constituent content height, and reduced energy consumption, reduced production cost.
Technical scheme of the present invention: the preparation method of decolouring, debitterized Momordica grosvenori extract is: Fructus Momordicae is cleaned, broken juice, fruit juice is centrifugal, and supernatant advances macroporous adsorbent resin, washed resin is colourless to effluent, reuse basic solvent eluting is washed to effluent then and is neutral, the ethanol elution of reuse 30%～65%, collect ethanol elution, reclaim ethanol, drying, promptly get decolour, debitterized Momordica grosvenori extract.Because Fructus Momordicae is when heating extraction, solution temperature is higher, and color is easily deepened, and is unfavorable for subsequent treatment; And Fructus Momordicae is directly squeezed the juice, can not only fully keep its effective ingredient, and the juice color is more shallow, be beneficial to subsequent decolorization, take off the flavor processing, product color is better.In addition, the used adsorbent resin of the present invention is active constituent-enriched, the effect of decolouring debitterize flavor is fine, and can further remove pigment and impurity with the basic solvent eluting, make the color and luster of product more shallow, purity is higher, therefore need only just can collect effective ingredient with the ethanol elution of low concentration (30%～65%) after the resin decolorization, and ethanol elution need not just can be realized purpose of the present invention with deodorization agent absorption once more.
Preparation method is more specifically: the Fructus Momordicae fresh fruit is cleaned, hot water completes, and (purpose was by the oxidase active in the high temperature destruction Fructus Momordicae in 1～2 minute, remove the bitter stink road of Fructus Momordicae epidermis), broken juice, residue with the water of 3～5 times of weight at room temperature (if elevated temperature can increase leaching impurity, increase energy consumption, it is then complicated more in operation to reduce temperature, increases production cost, and be unfavorable for leaching) soak squeezing 2～5 times; Fruit juice and pressed liquor are merged, centrifugal, supernatant advances macroporous adsorbent resin, and washed resin is colourless to effluent, the basic solvent eluting that the reuse weight resin is 2～10 times, be washed to effluent then and be neutral, 30%～65% ethanol elution that the reuse weight resin is 1～6 times is collected ethanol elution, and vacuum reclaims ethanol, drying, promptly get decolour, debitterized Momordica grosvenori extract.
In the above-mentioned preparation process, the temperature that hot water completes is 75～90 ℃.
Described macroporous adsorbent resin is ADS-5 type, ADS-4 type, ADS-7 type, ADS-8 type, ADS-F8 type or ADS-21 type macroporous adsorbent resin.These resins have good enrichment and decolouring simultaneously, take off the flavor effect, and its low price, can be repeatedly used, and cost is lower.
In order further to strengthen the effect of decolouring, debitterize flavor, improve product quality, the ethanol elution collected decolouring, the deodorization agent with Fructus Momordicae raw material weight 0.5%～5% can be carried out reclaiming ethanol again behind the adsorption-edulcoration.
Described decolouring, deodorization agent are neutral alumina or active carbon.
Described basic solvent is sodium bicarbonate, sodium carbonate or the sodium hydroxide solution (can not remove pigment and impurity effectively if alkalescence is too weak, alkalescence then can destroy, lose the effective ingredient in the extract too by force) of weight concentration 0.1%～2%.
Decolouring, debitterized Momordica grosvenori extract that the preparation method of the above decolouring, debitterized Momordica grosvenori extract prepares.
Compared with prior art, production technology of the present invention is simple, and production cost is low, produces to be easy to control, has avoided in the traditional handicraft low, the loaded down with trivial details shortcoming of column chromatography operation repeatedly of membrane filtration yield; Adopting squeezes the juice under the room temperature to extract replaces traditional heating extraction mode, has reduced energy consumption, and extract obtained is of light color, and impurity is few, and product quality is better; The adsorbent resin that is adopted has reached good decolouring, de-bittering effect in fully active constituent-enriched, not only improved the content of effective ingredient Momordica-Glycosides, and fully removed pigment and the impurity component in the extract by eluting; Products obtained therefrom is a white powder, and sweet taste is pure, the bitterness of extract and taste of Chinese medicine as none, and wherein the Fructus Momordicae dulcoside total glycosides weight content can reach that (UV method mensuration), Fructus Momordicae dulcoside V weight content can reach (HPLC method mensuration) more than 60% more than 90%.
(4) specific embodiment:
Embodiments of the invention 1: the preparation method of decolouring, debitterized Momordica grosvenori extract, its step is as follows:
1) the Fructus Momordicae fresh fruit is cleaned, 90 ℃ of hot water completed 1 minute;
2) the Fructus Momordicae broken juice after completing, residue place the pure water soaking at room temperature 4 times of 4 times of weight, squeezing, pressed liquor;
3) fruit juice and pressed liquor are merged, centrifugal remove impurity, supernatant advances ADS-21 type macroporous adsorbent resin;
4) the washing adsorbent resin is colourless to effluent, and 1% (weight percent concentration) sodium carbonate liquor eluting that the reuse weight resin is 6 times is removed pigment and impurity, is washed till effluent with pure water then and is neutral;
5) with the 65% alcoholic solution eluting removal pigment of 4 times of weight resins and the adsorbent resin of impurity, collect ethanol elution;
6) with the neutral alumina post absorption ethanol elution that Fructus Momordicae raw material weight 3% is housed, further remove pigment and bitterness;
7) vacuum reclaims the ethanol in the ethanol elution, spray drying, promptly get decolour, the Fructus Momordicae extract of debitterize.
Embodiments of the invention 2: preparation decolouring according to the following steps, debitterized Momordica grosvenori extract:
1) the Fructus Momordicae fresh fruit is cleaned, 85 ℃ of hot water completed 1 minute;
2) the Fructus Momordicae broken juice after completing, residue place the pure water soaking at room temperature 2 times of 5 times of weight, squeezing, pressed liquor;
3) fruit juice and pressed liquor are merged, centrifugal remove impurity is with ADS-7 type absorption with macroporous adsorbent resin centrifuged supernatant;
4) the washing adsorbent resin is colourless to effluent, and 2% (weight percent concentration) sodium bicarbonate solution eluting that the reuse weight resin is 10 times is removed pigment and impurity, is washed till effluent with pure water then and is neutral;
5) with the 35% alcoholic solution eluting removal pigment of 6 times of weight resins and the adsorbent resin of impurity, collect ethanol elution;
6) with the activated-charcoal column absorption ethanol elution that Fructus Momordicae raw material weight 1% is housed, further remove pigment and bitterness;
7) vacuum reclaims the ethanol in the ethanol elution, and spray drying must be decoloured, the Fructus Momordicae extract of debitterize.
Embodiments of the invention 3: preparation decolouring according to the following steps, debitterized Momordica grosvenori extract:
1) the Fructus Momordicae fresh fruit is cleaned, 80 ℃ of hot water completed 2 minutes;
2) the Fructus Momordicae broken juice after completing, residue place the water soaking at room temperature 5 times of 3 times of weight, squeezing, pressed liquor;
3) fruit juice and pressed liquor are merged, centrifugal remove impurity, supernatant advances ADS-8 type macroporous adsorbent resin;
4) the washing adsorbent resin is colourless to effluent, and 0.2% (weight percent concentration) sodium hydroxide solution eluting that the reuse weight resin is 2 times is removed pigment and impurity, is washed to effluent then and is neutral;
5) with the 50% alcoholic solution eluting removal pigment of 2 times of weight resins and the adsorbent resin of impurity, collect ethanol elution;
6) vacuum reclaims the ethanol in the ethanol elution, and drying must be decoloured, the Fructus Momordicae extract of debitterize.
Claims (8)
1. the preparation method of decolouring, debitterized Momordica grosvenori extract is characterized in that: Fructus Momordicae is cleaned broken juice, fruit juice is centrifugal, supernatant advances macroporous adsorbent resin, and washed resin is colourless to effluent, reuse basic solvent eluting, be washed to effluent then and be neutral, the ethanol elution of reuse 30%～65% is collected ethanol elution, reclaims ethanol, drying, promptly get decolour, debitterized Momordica grosvenori extract.
2. according to the preparation method of the described decolouring of claim 1, debitterized Momordica grosvenori extract, it is characterized in that: the Fructus Momordicae fresh fruit is cleaned, and hot water completed 1～2 minute, broken juice, and residue steeps 2～5 times with the hydroecium warm macerating of 3～5 times of weight, squeezing; Fruit juice and pressed liquor are merged, centrifugal, supernatant advances macroporous adsorbent resin, and washed resin is colourless to effluent, the basic solvent eluting that the reuse weight resin is 2～10 times, be washed to effluent then and be neutral, 30%～65% ethanol elution that the reuse weight resin is 1～6 times is collected ethanol elution, and vacuum reclaims ethanol, drying, promptly get decolour, debitterized Momordica grosvenori extract.
3. according to the preparation method of the described decolouring of claim 2, debitterized Momordica grosvenori extract, it is characterized in that: the temperature that hot water completes is 75～90 ℃.
4. according to the preparation method of claim 1 or 2 described decolourings, debitterized Momordica grosvenori extract, it is characterized in that: described macroporous adsorbent resin is ADS-5 type, ADS-4 type, ADS-7 type, ADS-8 type, ADS-F8 type or ADS-21 type macroporous adsorbent resin.
5. according to the preparation method of claim 1 or 2 described decolourings, debitterized Momordica grosvenori extract, it is characterized in that: reclaim ethanol again after the ethanol elution collected is adsorbed with decolouring, the deodorization agent of Fructus Momordicae raw material weight 0.5%～5%.
6. according to the preparation method of the described decolouring of claim 5, debitterized Momordica grosvenori extract, it is characterized in that: described decolouring, deodorization agent are neutral alumina or active carbon.
7. according to the preparation method of claim 1 or 2 described decolourings, debitterized Momordica grosvenori extract, it is characterized in that: described basic solvent is sodium bicarbonate, sodium carbonate or the sodium hydroxide solution of weight concentration 0.1%～2%.
8. decolouring, the debitterized Momordica grosvenori extract that the preparation method of each described decolouring, debitterized Momordica grosvenori extract prepares among the claim 1-7.
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| CN103837384A (en) * | 2014-01-21 | 2014-06-04 | 吉林大学 | Measuring method of soluble saccharide |
| CN104059959A (en) * | 2013-03-20 | 2014-09-24 | 广西壮族自治区中国科学院广西植物研究所 | Method for synthesizing sweet saponin with bitter and fallen grosvenor momordica fruit as raw material |
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- 2008-07-30 CN CNA2008103032386A patent/CN101327244A/en active Pending
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| US8962698B2 (en) | 2011-01-28 | 2015-02-24 | Tate & Lyle Ingredients Americas Llc | Rebaudioside-mogroside V blends |
| WO2012103074A3 (en) * | 2011-01-28 | 2012-12-13 | Tate & Lyle Ingredients Americas Llc | Rebaudioside-mogroside v blends |
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| CN106963801A (en) * | 2017-03-29 | 2017-07-21 | 桂林实力科技有限公司 | The preparation method of Fructus Monordicae extract |
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Open date: 20081224 |