CN103145869B - A kind of preparation method of the extract containing momordica grosvenori polysaccharide - Google Patents
A kind of preparation method of the extract containing momordica grosvenori polysaccharide Download PDFInfo
- Publication number
- CN103145869B CN103145869B CN201310100278.1A CN201310100278A CN103145869B CN 103145869 B CN103145869 B CN 103145869B CN 201310100278 A CN201310100278 A CN 201310100278A CN 103145869 B CN103145869 B CN 103145869B
- Authority
- CN
- China
- Prior art keywords
- concentrated solution
- grosvenor momordica
- extract
- momordica
- filtration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention take dried fructus momordicae as raw material, and adopt pure water to extract, concentrated, column chromatography decoloration and deodorization, Ethylene recov enzymolysis, decolouring, filtration, micro-filtration, nanofiltration, drying, obtain the Fructus Monordicae extract containing momordica grosvenori polysaccharide.The present invention will extract the later Grosvenor Momordica extracting solution recycling of Grosvenor Momordica glucoside material, and obtain a kind of extract containing momordica grosvenori polysaccharide, resource obtains comprehensive utilization.This patent combination technique is novel; After reclaiming the concentrated solution containing momordica grosvenori polysaccharide, add biological enzyme, concentrated solution viscosity can be reduced; Add activated carbon decolorizing; Selective membrane aperture is the micro-filtration of 0.45 μm is in order to removal of impurities, clarification, is convenient to nanofiltration membrane operation, reduces retaining and losing of momordica grosvenori polysaccharide; Selecting nanofiltration membrane to be to concentrate at normal temperatures, ensuring the quality of products, reducing costs.These technical combinations, are supported mutually together, acting in conjunction, can suitability for industrialized production.
Description
Technical field
The present invention relates to technical field of plant extraction, especially a kind of preparation method of extract of Grosvenor Momordica comprehensive utilization.
Background technology
Grosvenor Momordica nutritive value is very high, containing Grosvenor Momordica glucoside, in addition containing glucose, fructose, polysaccharide, protein, amino acid, flavonoid glycosides, vitamins C and multiple mineral element, there is taste sweet cool in nature, return lung, large intestine channel, moisten the lung and relieve the cough, the effect promoted the production of body fluid to quench thirst, be applicable to lung heat or xeropulmonary cough, it is thirsty etc. that Whooping cough and hot summer weather hinder Tianjin, the effect relaxed bowel in addition in addition.Grosvenor Momordica glucoside is mainly extracted in Grosvenor Momordica deep processing, and in the process extracting Grosvenor Momordica glucoside, also containing abundant nutritive substance in the Grosvenor Momordica extracting solution abandoned, such as momordica grosvenori polysaccharide is a kind of bioactive ingredients with enhancing body immunity.Chinese invention patent application publication number is CN101007042A, and name is called the Fructus Monordicae extract preparation method of a kind of decolouring, debitterize, discloses a kind of decolouring, debitterize, containing the method for preparing extractive of Grosvenor Momordica glucoside; This method is after extraction Grosvenor Momordica glucoside, and Grosvenor Momordica extracting solution can also reclaim, and extracts the composition containing momordica grosvenori polysaccharide further, obtains a kind of nutritious Fructus Monordicae extract, can be used as the raw material of healthcare products, nutritious prod.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of extracting solution from abandoning after extracting Grosvenor Momordica glucoside the preparation method reclaiming, extract the extract containing momordica grosvenori polysaccharide, can be obtained that a kind of solvability is good, cost is low, be convenient to the Fructus Monordicae extract containing momordica grosvenori polysaccharide of processing further by the method.
Technical scheme of the present invention is: a kind of preparation method of the extract containing momordica grosvenori polysaccharide, take dried fructus momordicae as raw material, employing pure water extracts, concentrated, column chromatography decoloration and deodorization, Ethylene recov enzymolysis, decolouring, filtration, micro-filtration, nanofiltration, drying, obtain the Fructus Monordicae extract containing momordica grosvenori polysaccharide.
Described pure water extracts, refer to that pure water Grosvenor Momordica being first placed in 6 times of its weight carries out decoction and obtains Luohanguo's residue and Aqueous extracts A, then pure water Luohanguo's residue being placed in 8 times of its weight carries out decoction and obtains Aqueous extracts B, is merged by A and B, filter, obtain Grosvenor Momordica just extract.
Described Ethylene recov enzymolysis, decolouring, refer to Grosvenor Momordica concentrated solution after macroporous resin adsorption, extraction Grosvenor Momordica glucoside material, reclaim the concentrated solution containing momordica grosvenori polysaccharide, then add the polygalacturonase of 0.01-0.02% by weight proportion, the papoid of 0.05-0.4%, stirs, control temperature is 40-55 DEG C, after 20-30 minute, then add the gac of 1.0%-2.0%, keep 30-60 minute.
Described filtration can be Plate Filtration, obtains cleaner liquid, and cross cleaner liquid and adopt instantaneous ultra high temperature sterilization (UHT) sterilization, sterilization temperature controls at 121-125 DEG C.
Described micro-filtration preferred film aperture is the microfiltration membrane of 0.45 μm; Obtain the filtrate by microfiltration membrane; When selecting membrane pore size to be the microfiltration membrane of 0.22 μm, in filtrate, momordica grosvenori polysaccharide content reduces, and has been trapped part.
Described nanofiltration, refers to by the filtrate by microfiltration membrane at normal temperatures by nanofiltration membrane, concentrated under playing normal temperature, obtains nanofiltration concentrated solution;
Described drying can be spraying dry, lyophilize or other drying meanss, will obtain obtaining nanofiltration concentrated solution drying, or pulverize further, and obtain the Fructus Monordicae extract containing momordica grosvenori polysaccharide.
Described Grosvenor Momordica pure water extracts, concentrated, column chromatography decoloration and deodorization, and filter, UHT sterilization, micro-filtration, nanofiltration, drying is all known technology.
The present invention has following advantages and characteristic:
1, comprehensive utilization of resources.To extract the later Grosvenor Momordica extracting solution recycling of Grosvenor Momordica glucoside material, obtain a kind of extract containing momordica grosvenori polysaccharide, can be processed into various nutritious prod and healthcare products further, Grosvenor Momordica resource obtains comprehensive utilization.
2, combination technique is novel.After reclaiming the concentrated solution containing momordica grosvenori polysaccharide, add polygalacturonase and papoid, depolymerized pectin and protein-basedly wait macromolecular substance, concentrated solution viscosity can be reduced, lay the first stone smoothly for subsequent nano-filtration operates, the solubleness of extract can also be improved.The gac added, main purpose is decolouring, guarantees that the extract color obtained is faint yellow, is convenient to be processed into other healthcare products, nutritious prod further.Crossing cleaner liquid adopts instantaneous ultra high temperature sterilization (UHT) sterilization to be in order to the polygalacturonase, the papoid that kill bacterium He add, is convenient to follow-up link quality and stablizes.Preferred film aperture is the microfiltration membrane of 0.45 μm is in order to removal of impurities, clarification, is convenient to nanofiltration membrane operation, increases the service life, can also reduce retaining and losing of momordica grosvenori polysaccharide as far as possible.Selecting nanofiltration membrane to be to concentrate at normal temperatures, ensuring the quality of products, reducing costs.These technical combinations, are supported mutually together, acting in conjunction, just can obtain that solvability is good, cost is low, extract containing momordica grosvenori polysaccharide.
Embodiment
The following stated example understands the present invention in detail.
Embodiment 1
1, pure water Grosvenor Momordica being first placed in 6 times of its weight decocts, and obtains Luohanguo's residue and Aqueous extracts A;
2, pure water Luohanguo's residue being placed in again 8 times of its weight decocts, and obtains Aqueous extracts B;
3, A and B is merged, filter, obtain Grosvenor Momordica just extract;
4, refer to first for Grosvenor Momordica extract to carry out concentrating under reduced pressure, obtain concentrated solution;
5, concentrated solution is carried out Plate Filtration, obtain concentrated solution clear liquid;
6, by Grosvenor Momordica concentrated solution clear liquid D101 styrene type interpolymer macroporous resin adsorption, decolouring; Reclaim Grosvenor Momordica concentrated solution clear liquid;
7, take the Grosvenor Momordica concentrated solution clear liquid 10 tons of recovery, control temperature 50 DEG C, add 1kg polygalacturonase and 5kg papoid, stir; Add 10kg powder-type gac after 20 minutes, continue stirring 40 minutes;
8, filter with flame filter press, obtained cleaner liquid 9.95 tons;
9, cross cleaner liquid and adopt instantaneous ultra high temperature sterilization (UHT) sterilization, sterilization temperature controls at 121-125 DEG C; Cooling, what obtain cooling crosses cleaner liquid 9.9 tons;
10, the ceramic microfiltration membrane being 0.45 μm by the cleaner liquid aperture excessively of cooling carries out micro-filtration, obtains micro-filtrate 9.86 tons;
11, micro-filtrate nano-filtration membrane equipment will be obtained carry out normal temperature nanofiltration and concentrate, collect nanofiltration membrane concentrated solution 980kg;
12, nanofiltration membrane concentrated solution is adopted centrifugal spray drying, obtaining color is the flaxen extract powder product containing momordica grosvenori polysaccharide.
Embodiment 2
1, pure water Grosvenor Momordica being first placed in 6 times of its weight decocts, and obtains Luohanguo's residue and Aqueous extracts A;
2, pure water Luohanguo's residue being placed in again 8 times of its weight decocts, and obtains Aqueous extracts B;
3, A and B is merged, filter, obtain Grosvenor Momordica just extract;
4, refer to first for Grosvenor Momordica extract to carry out concentrating under reduced pressure, obtain concentrated solution;
5, concentrated solution is carried out Plate Filtration, obtain concentrated solution clear liquid;
6, by Grosvenor Momordica concentrated solution clear liquid D201 styrene type interpolymer macroporous resin adsorption, decolouring; Reclaim Grosvenor Momordica concentrated solution clear liquid;
7, take the Grosvenor Momordica concentrated solution clear liquid 3 tons of recovery, control temperature 40 DEG C, add 0.6kg polygalacturonase and 8kg papoid, stir; Add 6kg gac after 30 minutes, continue stirring 60 minutes;
8, filter with flame filter press, obtained cleaner liquid;
9, cross cleaner liquid and adopt instantaneous ultra high temperature sterilization (UHT) sterilization, sterilization temperature controls at 121 DEG C; Cooling, what obtain cooling crosses cleaner liquid;
10, the ceramic microfiltration membrane being 0.45 μm by the cleaner liquid aperture excessively of cooling carries out micro-filtration, obtains micro-filtrate;
11, micro-filtrate nano-filtration membrane equipment will be obtained carry out normal temperature nanofiltration and concentrate, collect nanofiltration membrane concentrated solution;
12, nanofiltration membrane concentrated solution is adopted centrifugal spray drying, obtaining color is the flaxen extract containing momordica grosvenori polysaccharide.
Embodiment 3
1, pure water Grosvenor Momordica being first placed in 6 times of its weight decocts, and obtains Luohanguo's residue and Aqueous extracts A;
2, pure water Luohanguo's residue being placed in again 8 times of its weight decocts, and obtains Aqueous extracts B;
3, A and B is merged, filter, obtain Grosvenor Momordica just extract;
4, refer to first for Grosvenor Momordica extract to carry out concentrating under reduced pressure, obtain concentrated solution;
5, concentrated solution is carried out Plate Filtration, obtain concentrated solution clear liquid;
6, by Grosvenor Momordica concentrated solution clear liquid D301 styrene type interpolymer macroporous resin adsorption, decolouring; Reclaim Grosvenor Momordica concentrated solution clear liquid;
7, take the Grosvenor Momordica concentrated solution clear liquid 1000kg of recovery, control temperature 55 DEG C, adds 0.15kg polygalacturonase and 1kg papoid, stirs; Add 1kg gac after 30 minutes, continue stirring 30 minutes;
8, filter with small-sized flame filter press, obtained cleaner liquid;
9, cross cleaner liquid and adopt UHT sterilization, sterilization temperature controls at 125 DEG C, and what obtain cooling crosses cleaner liquid;
10, the little ceramic microfiltration membrane being 0.45 μm by the cleaner liquid aperture excessively of cooling carries out micro-filtration, obtains micro-filtrate;
11, the small-sized nano-filtration membrane equipment of micro-filtrate will be obtained carry out normal temperature nanofiltration and concentrate, collect nanofiltration membrane concentrated solution;
12, nanofiltration membrane concentrated solution is adopted lyophilize, through pulverizing after drying, just obtaining color is the flaxen extract containing momordica grosvenori polysaccharide.
Claims (1)
1. a preparation method for the extract containing momordica grosvenori polysaccharide take dried fructus momordicae as raw material, adopts following steps:
(1) pure water extracts; The pure water first Grosvenor Momordica being placed in 6 times of its weight decocts, and obtains Luohanguo's residue and Aqueous extracts A; The pure water that Luohanguo's residue is placed in 8 times of its weight more again decocts, and obtains Aqueous extracts B; A and B is merged, filters, obtain Grosvenor Momordica just extract;
(2) first for Grosvenor Momordica extract is concentrated, obtain carrying concentrated solution at the beginning of Grosvenor Momordica;
(3) by Grosvenor Momordica just extract employing column chromatography decoloration and deodorization, Grosvenor Momordica concentrated solution clear liquid is reclaimed;
It is characterized in that, also comprise the steps:
(4) in the Grosvenor Momordica concentrated solution clear liquid reclaimed, add the polygalacturonase of 0.01-0.02% by weight proportion, the papoid of 0.05-0.4%, stirs, control temperature is 40-55 DEG C, after 20-30 minute, then add the gac of 1.0%-2.0%, keep 30-60 minute;
(5) filter; Grosvenor Momordica concentrated solution after enzymolysis, decolouring is filtered, obtained cleaner liquid;
(6) cross cleaner liquid and adopt instantaneous ultra high temperature sterilization, cooling, what obtain cooling crosses cleaner liquid;
(7) by the cold filtration clear liquid membrane pore size obtained be the micro-filtrate membrane filtration of 0.45 μm, obtain the micro-filtrate by micro-filtration;
(8) nanofiltration, by the micro-filtrate nanofiltration by micro-filtration, obtains nanofiltration concentrated solution;
(9) dry, nanofiltration concentrated solution will be obtained through super-dry, obtain the Fructus Monordicae extract containing momordica grosvenori polysaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310100278.1A CN103145869B (en) | 2013-03-27 | 2013-03-27 | A kind of preparation method of the extract containing momordica grosvenori polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310100278.1A CN103145869B (en) | 2013-03-27 | 2013-03-27 | A kind of preparation method of the extract containing momordica grosvenori polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103145869A CN103145869A (en) | 2013-06-12 |
| CN103145869B true CN103145869B (en) | 2015-09-09 |
Family
ID=48544224
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310100278.1A Active CN103145869B (en) | 2013-03-27 | 2013-03-27 | A kind of preparation method of the extract containing momordica grosvenori polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103145869B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110105458A (en) * | 2019-03-25 | 2019-08-09 | 湖南华诚生物资源股份有限公司 | The method that polysaccharide and PEARLITOL 25C are extracted in waste liquid is extracted from mogroside |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ549739A (en) | 2006-09-07 | 2009-01-31 | Biovittoria Ltd | Sweetening compositions and processes for preparing them |
| WO2016088700A1 (en) * | 2014-12-02 | 2016-06-09 | 横浜油脂工業株式会社 | Method for manufacturing composition containing sweetness component from siraitia grosvenorii |
| CN106261001A (en) * | 2015-06-02 | 2017-01-04 | 桂林电子科技大学 | A kind of preparation method of Fructus Momordicae compound extract |
| CN106333206B (en) * | 2016-08-26 | 2019-10-29 | 桂林莱茵生物科技股份有限公司 | A kind of production method of honey taste arhat juice |
| US11576412B2 (en) | 2016-10-24 | 2023-02-14 | Guilin Gfs Monk Fruit Corporation | Extracts from fruits of the Cucurbitaceae family, and methods of preparing thereof |
| CN107459836A (en) * | 2017-08-02 | 2017-12-12 | 刘启华 | A kind of preparation method of Momordica grosvenori caramel colorant |
| CN109674843B (en) * | 2019-01-30 | 2022-03-22 | 湖南绿蔓生物科技股份有限公司 | Extraction and purification method for comprehensively utilizing dried momordica grosvenori |
| CN110652005A (en) * | 2019-09-25 | 2020-01-07 | 上海诺德生物实业有限公司 | Method for preparing hovenia dulcis thunb extract by enzymolysis method |
| CN110973428A (en) * | 2019-12-20 | 2020-04-10 | 广西健美乐食品有限公司 | Danzhu liquid for clearing heat and reducing internal heat and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101007042A (en) * | 2007-02-06 | 2007-08-01 | 桂林莱茵生物科技股份有限公司 | A preparation method of debittered and discolored siraitia grosvenorii extracts |
| CN102742906A (en) * | 2012-07-18 | 2012-10-24 | 何伟平 | Momordica grosvenori drink with low glycemic index and anti-fatigue action |
-
2013
- 2013-03-27 CN CN201310100278.1A patent/CN103145869B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101007042A (en) * | 2007-02-06 | 2007-08-01 | 桂林莱茵生物科技股份有限公司 | A preparation method of debittered and discolored siraitia grosvenorii extracts |
| CN102742906A (en) * | 2012-07-18 | 2012-10-24 | 何伟平 | Momordica grosvenori drink with low glycemic index and anti-fatigue action |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110105458A (en) * | 2019-03-25 | 2019-08-09 | 湖南华诚生物资源股份有限公司 | The method that polysaccharide and PEARLITOL 25C are extracted in waste liquid is extracted from mogroside |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103145869A (en) | 2013-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103145869B (en) | A kind of preparation method of the extract containing momordica grosvenori polysaccharide | |
| US10743573B2 (en) | Method for extracting high-purity mogroside V from Siraitia grosvenorii | |
| CN106243172B (en) | A method of extracting black fruit fructus lycii anthocyanin | |
| CN101633676B (en) | Method for preparing high-purity stachyose by using plant chromatographic separation technology | |
| CN107397103A (en) | A kind of preparation method of Momordica grosvenori decolouring flavor inspissated juice | |
| CN105669800B (en) | A kind of combined extracting essential oil from citrus, pectin, aurantiamarin, the method for synephrine and limonin | |
| CN100548150C (en) | The preparation method of coconut extractive | |
| CN109674843B (en) | Extraction and purification method for comprehensively utilizing dried momordica grosvenori | |
| CN108752231B (en) | Method for extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenol | |
| CN104069151B (en) | A kind of high method for preparing extractive of content of mogroside IV | |
| CN101999491A (en) | Process method for extracting tea polyphenol from Fuzhuan tea | |
| CN108690099A (en) | A method of the extraction separation procyanidine from black fruit fructus lycii | |
| CN100447206C (en) | Method of extracting and preparing yellow pigment of pagodatree flower | |
| CN105238551A (en) | Method for supercritical CO2 extraction and purification of camellia seed oil | |
| CN109758498B (en) | Preparation method of olive leaf extract | |
| CN113637038B (en) | Method for extracting sweet tea glycoside and sweet tea polyphenol without bitter taste from sweet tea leaves | |
| CN104001033A (en) | Preparation process of roxburgh anoectochilus terminal bud concentrated solution | |
| JP6768970B2 (en) | How to prepare rubusoside | |
| CN110917240B (en) | Continuous method for separating multiple effective components from cyclocarya paliurus | |
| CN102579564A (en) | Preparing method of Tibetan capillaris effective part extractives | |
| CN104069152A (en) | Preparation method of extract with high mogroside IV content | |
| CN106072638A (en) | A kind of production method of instant Lepidinm meyenii Walp sugar | |
| CN110240584A (en) | The method for separating procyanidine, anthocyanidin and ferulic acid simultaneously from purple rice rice bran | |
| CN105315705B (en) | The method of extraction purification Gardenia Yellow in cape jasmine | |
| CN111978365B (en) | Preparation method of mogroside V-containing mogroside |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20130612 Assignee: GUANGZHOU CANYON BIOTECHNOLOGY CO.,LTD Assignor: Guilin Electronic Science and Technology Univ. Contract record no.: 2016450000026 Denomination of invention: Preparation method of extract containing siraitia grosvenori polysaccharide Granted publication date: 20150909 License type: Exclusive License Record date: 20161213 |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170413 Address after: Huadu District, Guangdong city of Guangzhou province Xinya street 510800 village two team wide blue Ke Industrial Park Building No. 1 Patentee after: GUANGZHOU CANYON BIOTECHNOLOGY CO.,LTD Address before: 541004 the Guangxi Zhuang Autonomous Region golden Guilin Qixing District Road No. 1 Patentee before: Guilin University of Electronic Technology |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201125 Address after: No. 3, longhui Industrial Road, Huadu District, Huadu District, Guangzhou, Guangdong province (can be used as a workshop) Patentee after: GUANGZHOU CIKANG BIOTECHNOLOGY Co.,Ltd. Address before: Huadu District, Guangdong city of Guangzhou province Xinya street 510800 village two team wide blue Ke Industrial Park Building No. 1 Patentee before: GUANGZHOU CANYON BIOTECHNOLOGY Co.,Ltd. |