CN103145869A - Preparation method of extract containing siraitia grosvenori polysaccharide - Google Patents

Preparation method of extract containing siraitia grosvenori polysaccharide Download PDF

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Publication number
CN103145869A
CN103145869A CN2013101002781A CN201310100278A CN103145869A CN 103145869 A CN103145869 A CN 103145869A CN 2013101002781 A CN2013101002781 A CN 2013101002781A CN 201310100278 A CN201310100278 A CN 201310100278A CN 103145869 A CN103145869 A CN 103145869A
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concentrated solution
grosvenor momordica
extract
siraitia grosvenori
nanofiltration
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CN103145869B (en
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周治德
李桂银
黄勇
陈真诚
卓振松
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GUANGZHOU CIKANG BIOTECHNOLOGY Co.,Ltd.
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Guilin University of Electronic Technology
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Abstract

The invention relates to a preparation method of an extract containing siraitia grosvenori polysaccharide. The preparation method taking dried siraitia grosvenori as a raw material includes the steps of carrying out extraction and concentration by adopting purified water, carrying out decolorization and deodorization by column chromatography, carrying out enzymolysis and decolorization, filtration, microfiltration, nanofiltration and drying on recovered solution, and thus obtaining the siraitia grosvenori extract containing siraitia grosvenori polysaccharide. The preparation method obtains the extract containing siraitia grosvenori polysaccharide by reusing siraitia grosvenori extracting solution after extracting siraitia grosvenori glycosides materials, so that resources are comprehensively utilized. The combined technology of the invention is novel; the viscosity of concentrate can be reduced by adding bio-enzyme after recovering concentrate containing siraitia grosvenori polysaccharide; active carbon is added for decolorization; the microfiltration with the membrane aperture being 0.45 micron is selected for removing impurities and clarifying so as to facilitate nanofiltration membrane operation and reduce the entrapment and loss of the siraitia grosvenori polysaccharide; and a nanofiltration membrane is selected for concentrating at room temperature so as to ensure the quality and reduce the cost. The technologies are combined together and mutually supported to achieve combined action, thus being suitable for industrial production.

Description

A kind of preparation method who contains the extract of momordica grosvenori polysaccharide
Technical field
The present invention relates to technical field of plant extraction, especially a kind of preparation method of extract of Grosvenor Momordica comprehensive utilization.
Background technology
The Grosvenor Momordica nutritive value is very high, contain the Grosvenor Momordica glucoside, contain in addition glucose, fructose, polysaccharide, protein, amino acid, flavonoid glycosides, vitamins C and multiple mineral element, have flavor sweet cool in nature, return lung, large intestine channel, moisten the lung and relieve the cough, the effect that promotes the production of body fluid to quench thirst, be applicable to lung heat or xeropulmonary cough, it is thirsty etc. that Whooping cough and hot summer weather are hindered Tianjin, the effect that relaxes bowel in addition in addition.The Grosvenor Momordica deep processing is mainly to extract the Grosvenor Momordica glucoside, in the process of extracting the Grosvenor Momordica glucoside, also contains abundant nutritive substance in the Grosvenor Momordica extracting solution that abandons, and such as momordica grosvenori polysaccharide, is a kind of bioactive ingredients that strengthens body immunity that has.The Chinese invention patent application publication number is CN101007042A, and name is called the Fructus Monordicae extract preparation method of a kind of decolouring, debitterize, discloses a kind of decolouring, debitterize, contains the method for preparing extractive of Grosvenor Momordica glucoside; This method is after extracting the Grosvenor Momordica glucoside, and the Grosvenor Momordica extracting solution can also reclaim, and further extracts the composition that contains momordica grosvenori polysaccharide, obtains a kind of nutritious Fructus Monordicae extract, can be used as the raw material of healthcare products, nutritious prod.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of preparation method who reclaims, extracts the extract that contains momordica grosvenori polysaccharide from extract the extracting solution that the Grosvenor Momordica glucoside abandons later on, can access by the method that a kind of solvability is good, cost is low, be convenient to the further Fructus Monordicae extract that contains momordica grosvenori polysaccharide of processing.
Technical scheme of the present invention is: a kind of preparation method who contains the extract of momordica grosvenori polysaccharide, take dried fructus momordicae as raw material, the employing pure water extracts, concentrated, the column chromatography decoloration and deodorization, reclaim liquid enzymolysis, decolouring, filtration, micro-filtration, nanofiltration, drying obtain containing the Fructus Monordicae extract of momordica grosvenori polysaccharide.
Described pure water extracts, refer to the pure water that Grosvenor Momordica first is placed in 6 times of its weight is decocted and obtain arhat pomace and water extraction liquid A, then the pure water that the arhat pomace is placed in 8 times of its weight is decocted obtain water extraction liquid B, A and B are merged, filter, obtain just extract of Grosvenor Momordica.
Described recovery liquid enzymolysis, decolouring, refer to after Grosvenor Momordica concentrated solution process macroporous resin adsorption, extraction Grosvenor Momordica glucoside material, recovery contains the concentrated solution of momordica grosvenori polysaccharide, then add by weight proportion the polygalacturonase of 0.01-0.02%, the papoid of 0.05-0.4% stirs, controlling temperature is 40-55 ℃, after 20-30 minute, then add the gac of 1.0%-2.0%, kept 30-60 minute.
Described filtration can be Plate Filtration, obtains cleaner liquid, crosses cleaner liquid and adopts instantaneous ultra high temperature sterilization (UHT) sterilization, and sterilization temperature is controlled at 121-125 ℃.
Described micro-filtration preferred film aperture is the microfiltration membrane of 0.45 μ m; Obtain the filtrate by microfiltration membrane; When selecting membrane pore size to be the microfiltration membrane of 0.22 μ m, in filtrate, momordica grosvenori polysaccharide content reduces, and has been trapped part.
Described nanofiltration refers to the filtrate by microfiltration membrane is passed through nanofiltration membrane at normal temperatures, plays under normal temperature concentrating, and obtains the nanofiltration concentrated solution;
Described drying can be spraying drying, lyophilize or other drying meanss, will obtain getting nanofiltration concentrated solution drying, perhaps further pulverizes, and obtains containing the Fructus Monordicae extract of momordica grosvenori polysaccharide.
Described Grosvenor Momordica pure water extracts, and is concentrated, and the column chromatography decoloration and deodorization filters, the UHT sterilization, and micro-filtration, nanofiltration, drying is all known technology.
The present invention has following advantages and characteristic:
1, comprehensive utilization of resources.With extracting the later Grosvenor Momordica extracting solution recycling of Grosvenor Momordica glucoside material, obtain a kind of extract that contains momordica grosvenori polysaccharide, can further be processed into various nutritious prod and healthcare products, the Grosvenor Momordica resource has obtained comprehensive utilization.
2, combination technique is novel.After recovery contains the concentrated solution of momordica grosvenori polysaccharide, add polygalacturonase and papoid, depolymerized pectin and the protein-based macromolecular substance that waits can reduce concentrated solution viscosity, lay the first stone smoothly for follow-up nanofiltration operates, and can also improve the solubleness of extract.The gac that adds, main purpose are decolourings, guarantee that the extract color that obtains is faint yellow, is convenient to further be processed into other healthcare products, nutritious prod.Crossing the sterilization of cleaner liquid employing instantaneous ultra high temperature sterilization (UHT) is for killing bacteria and the polygalacturonase, the papoid that add, is convenient to follow-up link quality stable.The preferred film aperture is that the microfiltration membrane of 0.45 μ m is for removal of impurities, clarification, is convenient to the nanofiltration membrane operation, increases the service life, and can also reduce holding back and losing of momordica grosvenori polysaccharide as far as possible.Selecting nanofiltration membrane is for concentrated at normal temperatures, ensures the quality of products, and reduces costs.These technical combinations are supported mutually together, and acting in conjunction can obtain just that solvability is good, cost is low, contain the extract of momordica grosvenori polysaccharide.
Embodiment
The following stated example understands the present invention in detail.
Embodiment 1
1, the pure water that Grosvenor Momordica first is placed in 6 times of its weight decocts, and obtains arhat pomace and water extraction liquid A;
2, the pure water that the arhat pomace is placed in again 8 times of its weight decocts, and obtains water extraction liquid B;
3, A and B are merged, filter, obtain just extract of Grosvenor Momordica;
4, refer to the first extract of Grosvenor Momordica is carried out concentrating under reduced pressure, obtain concentrated solution;
5, concentrated solution is carried out Plate Filtration, obtain the concentrated solution clear liquid;
6, Grosvenor Momordica concentrated solution clear liquid is used D101 styrene type interpolymer macroporous resin adsorption, decolouring; Reclaim Grosvenor Momordica concentrated solution clear liquid;
7, take 10 tons of the Grosvenor Momordica concentrated solution clear liquids of recovery, control temperature 50 C, add 1kg polygalacturonase and 5kg papoid, stir; Add 10kg powder-type gac after 20 minutes, continue to stir 40 minutes;
8, filter with flame filter press, obtained 9.95 tons of cleaner liquids;
9, cross cleaner liquid and adopt instantaneous ultra high temperature sterilization (UHT) sterilization, sterilization temperature is controlled at 121-125 ℃; Cooling, obtain 9.9 tons of cooling cleaner liquids excessively;
10, the ceramic microfiltration membrane that is 0.45 μ m with cooling cleaner liquid excessively with the aperture carries out micro-filtration, obtains 9.86 tons of micro-filtrates;
11, obtaining micro-filtrate, to carry out the normal temperature nanofiltration with nano-filtration membrane equipment concentrated, collects nanofiltration membrane concentrated solution 980kg;
12, the nanofiltration membrane concentrated solution is adopted centrifugal spray drying, obtaining color is the flaxen extract powder product that contains momordica grosvenori polysaccharide.
Embodiment 2
1, the pure water that Grosvenor Momordica first is placed in 6 times of its weight decocts, and obtains arhat pomace and water extraction liquid A;
2, the pure water that the arhat pomace is placed in again 8 times of its weight decocts, and obtains water extraction liquid B;
3, A and B are merged, filter, obtain just extract of Grosvenor Momordica;
4, refer to the first extract of Grosvenor Momordica is carried out concentrating under reduced pressure, obtain concentrated solution;
5, concentrated solution is carried out Plate Filtration, obtain the concentrated solution clear liquid;
6, Grosvenor Momordica concentrated solution clear liquid is used D201 styrene type interpolymer macroporous resin adsorption, decolouring; Reclaim Grosvenor Momordica concentrated solution clear liquid;
7, take 3 tons of the Grosvenor Momordica concentrated solution clear liquids of recovery, control 40 ℃ of temperature, add 0.6kg polygalacturonase and 8kg papoid, stir; Add the 6kg gac after 30 minutes, continue to stir 60 minutes;
8, filter with flame filter press, obtained cleaner liquid;
9, cross cleaner liquid and adopt instantaneous ultra high temperature sterilization (UHT) sterilization, sterilization temperature is controlled at 121 ℃; Cooling, obtain the cooling cleaner liquid of crossing;
10, the ceramic microfiltration membrane that is 0.45 μ m with cooling cleaner liquid excessively with the aperture carries out micro-filtration, obtains micro-filtrate;
11, obtaining micro-filtrate, to carry out the normal temperature nanofiltration with nano-filtration membrane equipment concentrated, collects the nanofiltration membrane concentrated solution;
12, the nanofiltration membrane concentrated solution is adopted centrifugal spray drying, obtaining color is the flaxen extract that contains momordica grosvenori polysaccharide.
Embodiment 3
1, the pure water that Grosvenor Momordica first is placed in 6 times of its weight decocts, and obtains arhat pomace and water extraction liquid A;
2, the pure water that the arhat pomace is placed in again 8 times of its weight decocts, and obtains water extraction liquid B;
3, A and B are merged, filter, obtain just extract of Grosvenor Momordica;
4, refer to the first extract of Grosvenor Momordica is carried out concentrating under reduced pressure, obtain concentrated solution;
5, concentrated solution is carried out Plate Filtration, obtain the concentrated solution clear liquid;
6, Grosvenor Momordica concentrated solution clear liquid is used D301 styrene type interpolymer macroporous resin adsorption, decolouring; Reclaim Grosvenor Momordica concentrated solution clear liquid;
7, take the Grosvenor Momordica concentrated solution clear liquid 1000kg of recovery, control 55 ℃ of temperature, add 0.15kg polygalacturonase and 1kg papoid, stir; Add the 1kg gac after 30 minutes, continue to stir 30 minutes;
8, filter with small-sized flame filter press, obtained cleaner liquid;
9, cross cleaner liquid and adopt the UHT sterilization, sterilization temperature is controlled at 125 ℃, obtains the cooling cleaner liquid of crossing;
10, the little ceramic microfiltration membrane that is 0.45 μ m with cooling cleaner liquid excessively with the aperture carries out micro-filtration, obtains micro-filtrate;
11, obtaining micro-filtrate, to carry out the normal temperature nanofiltration with small-sized nano-filtration membrane equipment concentrated, collects the nanofiltration membrane concentrated solution;
12, the nanofiltration membrane concentrated solution is adopted lyophilize, dry by pulverizing, just obtaining color is the flaxen extract that contains momordica grosvenori polysaccharide.

Claims (1)

1. preparation method who contains the extract of momordica grosvenori polysaccharide, take dried fructus momordicae as raw material, adopt following steps:
(1) pure water extracts; The pure water that first Grosvenor Momordica is placed in 6 times of its weight decocts, and obtains arhat pomace and water extraction liquid A; The arhat pomace pure water that is placed in again 8 times of its weight decocts again, obtains water extraction liquid B; A and B are merged, filter, obtain just extract of Grosvenor Momordica;
(2) just extract is concentrated with Grosvenor Momordica, obtains Grosvenor Momordica and just carries concentrated solution;
(3) with just extract employing of Grosvenor Momordica column chromatography decoloration and deodorization, reclaim Grosvenor Momordica concentrated solution clear liquid;
It is characterized in that, also comprise the steps:
(4) add by weight proportion the polygalacturonase of 0.01-0.02% in the Grosvenor Momordica concentrated solution clear liquid that reclaims, the papoid of 0.05-0.4% stirs, controlling temperature is 40-55 ℃, after 20-30 minute, then add the gac of 1.0%-2.0%, kept 30-60 minute;
(5) filter; Grosvenor Momordica concentrated solution after enzymolysis, decolouring is filtered, obtained cleaner liquid;
(6) cross cleaner liquid and adopt instantaneous ultra high temperature sterilization, cooling, obtain the cooling cleaner liquid of crossing;
(7) be the micro-filtrate membrane filtration of 0.45 μ m with the cold filtration clear liquid membrane pore size that obtains, obtain the micro-filtrate by micro-filtration;
(8) nanofiltration will by the micro-filtrate nanofiltration of micro-filtration, obtain the nanofiltration concentrated solution;
(9) drying will obtain the nanofiltration concentrated solution through super-dry, obtain containing the Fructus Monordicae extract of momordica grosvenori polysaccharide.
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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106261001A (en) * 2015-06-02 2017-01-04 桂林电子科技大学 A kind of preparation method of Fructus Momordicae compound extract
CN106333206A (en) * 2016-08-26 2017-01-18 桂林莱茵生物科技股份有限公司 Method for preparing momordica grosvenor juice with honey taste
JPWO2016088700A1 (en) * 2014-12-02 2017-10-19 横浜油脂工業株式会社 Method for producing composition containing Rahan fruit sweetener
CN107459836A (en) * 2017-08-02 2017-12-12 刘启华 A kind of preparation method of Momordica grosvenori caramel colorant
WO2018077140A1 (en) * 2016-10-24 2018-05-03 Guilin Gfs Monk Fruit Corporation Extracts from fruits of the cucurbitaceae family, and methods of preparing thereof
CN109674843A (en) * 2019-01-30 2019-04-26 湖南绿蔓生物科技股份有限公司 A kind of method for extraction and purification of dried fructus momordicae comprehensive utilization
CN110652005A (en) * 2019-09-25 2020-01-07 上海诺德生物实业有限公司 Method for preparing hovenia dulcis thunb extract by enzymolysis method
CN110973428A (en) * 2019-12-20 2020-04-10 广西健美乐食品有限公司 Danzhu liquid for clearing heat and reducing internal heat and preparation method thereof
CN110105458B (en) * 2019-03-25 2021-06-25 湖南华诚生物资源股份有限公司 Method for extracting polysaccharide and D-mannitol from mogroside extraction waste liquid
US11464247B2 (en) 2006-09-07 2022-10-11 Guilin Gfs Monk Fruit Corp. Sweetening compositions and processes for preparing them
CN115715891A (en) * 2022-11-11 2023-02-28 湖南绿蔓生物科技股份有限公司 Continuous method for separating multiple effective components from fructus Siraitiae Grosvenorii

Citations (2)

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CN101007042A (en) * 2007-02-06 2007-08-01 桂林莱茵生物科技股份有限公司 A preparation method of debittered and discolored siraitia grosvenorii extracts
CN102742906A (en) * 2012-07-18 2012-10-24 何伟平 Momordica grosvenori drink with low glycemic index and anti-fatigue action

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101007042A (en) * 2007-02-06 2007-08-01 桂林莱茵生物科技股份有限公司 A preparation method of debittered and discolored siraitia grosvenorii extracts
CN102742906A (en) * 2012-07-18 2012-10-24 何伟平 Momordica grosvenori drink with low glycemic index and anti-fatigue action

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11464247B2 (en) 2006-09-07 2022-10-11 Guilin Gfs Monk Fruit Corp. Sweetening compositions and processes for preparing them
JPWO2016088700A1 (en) * 2014-12-02 2017-10-19 横浜油脂工業株式会社 Method for producing composition containing Rahan fruit sweetener
CN106261001A (en) * 2015-06-02 2017-01-04 桂林电子科技大学 A kind of preparation method of Fructus Momordicae compound extract
CN106333206A (en) * 2016-08-26 2017-01-18 桂林莱茵生物科技股份有限公司 Method for preparing momordica grosvenor juice with honey taste
WO2018077140A1 (en) * 2016-10-24 2018-05-03 Guilin Gfs Monk Fruit Corporation Extracts from fruits of the cucurbitaceae family, and methods of preparing thereof
CN108471789A (en) * 2016-10-24 2018-08-31 桂林吉福思罗汉果有限公司 Extract and preparation method thereof from Curcurbitaceae fruit
US11576412B2 (en) 2016-10-24 2023-02-14 Guilin Gfs Monk Fruit Corporation Extracts from fruits of the Cucurbitaceae family, and methods of preparing thereof
CN107459836A (en) * 2017-08-02 2017-12-12 刘启华 A kind of preparation method of Momordica grosvenori caramel colorant
CN109674843A (en) * 2019-01-30 2019-04-26 湖南绿蔓生物科技股份有限公司 A kind of method for extraction and purification of dried fructus momordicae comprehensive utilization
CN109674843B (en) * 2019-01-30 2022-03-22 湖南绿蔓生物科技股份有限公司 Extraction and purification method for comprehensively utilizing dried momordica grosvenori
CN110105458B (en) * 2019-03-25 2021-06-25 湖南华诚生物资源股份有限公司 Method for extracting polysaccharide and D-mannitol from mogroside extraction waste liquid
CN110652005A (en) * 2019-09-25 2020-01-07 上海诺德生物实业有限公司 Method for preparing hovenia dulcis thunb extract by enzymolysis method
CN110973428A (en) * 2019-12-20 2020-04-10 广西健美乐食品有限公司 Danzhu liquid for clearing heat and reducing internal heat and preparation method thereof
CN115715891A (en) * 2022-11-11 2023-02-28 湖南绿蔓生物科技股份有限公司 Continuous method for separating multiple effective components from fructus Siraitiae Grosvenorii

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