CN111918972A - 评估存在或不存在有复制能力的病毒的方法和试剂 - Google Patents

评估存在或不存在有复制能力的病毒的方法和试剂 Download PDF

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Publication number
CN111918972A
CN111918972A CN201980021932.5A CN201980021932A CN111918972A CN 111918972 A CN111918972 A CN 111918972A CN 201980021932 A CN201980021932 A CN 201980021932A CN 111918972 A CN111918972 A CN 111918972A
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seq
sequence
gene
oligonucleotide primer
viral
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Chinese (zh)
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R·贝瑞
E·韦伯
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Juno Therapeutics Inc
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Juno Therapeutics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/161—HIV-1 ; HIV-2 gag-pol, e.g. p55, p24/25, p17/18, p7, p6, p66/68, p51/52, p31/34, p32, p40
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/162—HIV-1 ; HIV-2 env, e.g. gp160, gp110/120, gp41, V3, peptid T, CD4-Binding site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Communicable Diseases (AREA)
  • AIDS & HIV (AREA)
  • Oncology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980021932.5A 2018-01-31 2019-01-31 评估存在或不存在有复制能力的病毒的方法和试剂 Pending CN111918972A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862624801P 2018-01-31 2018-01-31
US62/624,801 2018-01-31
PCT/US2019/016194 WO2019152747A1 (en) 2018-01-31 2019-01-31 Methods and reagents for assessing the presence or absence of replication competent virus

Publications (1)

Publication Number Publication Date
CN111918972A true CN111918972A (zh) 2020-11-10

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US (2) US11535903B2 (enExample)
EP (1) EP3746569A1 (enExample)
JP (1) JP2021511802A (enExample)
CN (1) CN111918972A (enExample)
WO (1) WO2019152747A1 (enExample)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112505126A (zh) * 2020-12-16 2021-03-16 中国科学院重庆绿色智能技术研究院 金属离子在影响诱导HIV-1的Tat蛋白水解中的应用及其检测方法
CN112877475A (zh) * 2021-02-19 2021-06-01 北京鼎成肽源生物技术有限公司 一种检测rcl的引物探针组合、试剂盒和检测方法
CN113403343A (zh) * 2021-04-30 2021-09-17 吉林大学 一种h3n2与h9n2亚型禽流感二价嵌合型病毒样颗粒的制备
CN113699278A (zh) * 2021-09-17 2021-11-26 深圳因诺免疫有限公司 一种基于q-PCR法检测复制型逆转录病毒的引物、试剂盒及检测方法
CN114958983A (zh) * 2021-11-04 2022-08-30 湖州申科生物技术有限公司 分析和检测慢病毒载体介导的目的基因的拷贝数的方法
CN117737303A (zh) * 2023-12-14 2024-03-22 浙江恒驭生物科技有限公司 细胞治疗产品中复制型逆转录病毒rcr-galv的检测组合物、方法及试剂盒
TWI852072B (zh) * 2022-08-08 2024-08-11 新加坡商台達電子國際（新加坡）私人有限公司 檢測病毒感染性的樣本前處理試劑組合及方法

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Publication number Priority date Publication date Assignee Title
MA45783A (fr) 2016-07-29 2019-06-05 Juno Therapeutics Inc Procédés d'évaluation de la présence ou de l'absence d'un virus compétent pour la réplication
JP2021511802A (ja) 2018-01-31 2021-05-13 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法および試薬
CN111910015A (zh) * 2019-05-08 2020-11-10 深圳宾德生物技术有限公司 一种检测复制型慢病毒的探针、引物对、荧光定量pcr试剂盒及方法
JP7726874B2 (ja) * 2019-10-11 2025-08-20 カタログ テクノロジーズ， インコーポレイテッド 核酸セキュリティーおよび認証
GB202003412D0 (en) 2020-03-09 2020-04-22 Oxford Biomedica Ltd Replication competent virus assay
CN113528706A (zh) * 2021-07-13 2021-10-22 苏州博腾生物制药有限公司 三通道双淬灭探针检测可复制性慢病毒的试剂及其检测方法
CN114369687A (zh) * 2022-01-29 2022-04-19 宁波熙宁检测技术有限公司 同时检测VSVG和ALB基因的多重qPCR反应体系的建立方法
CN114410844A (zh) * 2022-01-29 2022-04-29 宁波熙宁检测技术有限公司 多重qPCR检测GALV和ALB拷贝数判定CAR-T细胞产品中RCR阴阳性的方法
KR102804750B1 (ko) * 2022-10-20 2025-05-12 성균관대학교산학협력단 바이러스 생산 세포 검출용 멀티플렉스 pcr 프라이머 및 프로브
CN116219069A (zh) * 2022-11-11 2023-06-06 湖州申科生物技术股份有限公司 一种检测可复制性慢病毒的探针、引物、试剂盒及其应用
CN116970737A (zh) * 2023-04-27 2023-10-31 北京艺升医疗科技有限公司 一种复制型慢病毒检测方法

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112505126A (zh) * 2020-12-16 2021-03-16 中国科学院重庆绿色智能技术研究院 金属离子在影响诱导HIV-1的Tat蛋白水解中的应用及其检测方法
CN112505126B (zh) * 2020-12-16 2023-03-24 中国科学院重庆绿色智能技术研究院 检测金属离子影响诱导HIV-1的Tat蛋白水解的方法
CN112877475A (zh) * 2021-02-19 2021-06-01 北京鼎成肽源生物技术有限公司 一种检测rcl的引物探针组合、试剂盒和检测方法
CN113403343A (zh) * 2021-04-30 2021-09-17 吉林大学 一种h3n2与h9n2亚型禽流感二价嵌合型病毒样颗粒的制备
CN113699278A (zh) * 2021-09-17 2021-11-26 深圳因诺免疫有限公司 一种基于q-PCR法检测复制型逆转录病毒的引物、试剂盒及检测方法
CN114958983A (zh) * 2021-11-04 2022-08-30 湖州申科生物技术有限公司 分析和检测慢病毒载体介导的目的基因的拷贝数的方法
TWI852072B (zh) * 2022-08-08 2024-08-11 新加坡商台達電子國際（新加坡）私人有限公司 檢測病毒感染性的樣本前處理試劑組合及方法
CN117737303A (zh) * 2023-12-14 2024-03-22 浙江恒驭生物科技有限公司 细胞治疗产品中复制型逆转录病毒rcr-galv的检测组合物、方法及试剂盒

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US11535903B2 (en) 2022-12-27
US20210164062A1 (en) 2021-06-03
JP2021511802A (ja) 2021-05-13
US12264371B2 (en) 2025-04-01
US20230193411A1 (en) 2023-06-22
WO2019152747A1 (en) 2019-08-08
EP3746569A1 (en) 2020-12-09

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