CN111908966A - Brown algae polyphenol trace element compound fertilizer and preparation method thereof - Google Patents
Brown algae polyphenol trace element compound fertilizer and preparation method thereof Download PDFInfo
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- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05G3/50—Surfactants; Emulsifiers
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses a brown algae polyphenol trace element compound fertilizer and a preparation method thereof. The invention comprises the following components: 35-70 parts of brown algae polyphenol extracting solution, 800.1-5 parts of tween, 10-20 parts of urea, 10-20 parts of monopotassium phosphate, 0.1-1 part of EDTA-Cu, 0.1-1 part of EDTA-Fe, 0.1-1 part of EDTA-Mn, 0.1-1 part of EDTA-Mg, 0.1-2 parts of boric acid, 0.01-0.1 part of ammonium molybdate heptahydrate and 1-20 parts of mannitol; the brown algae polyphenol extracting solution is obtained by grinding, enzymolysis by a biological enzyme complexing agent, extraction and purification. The brown algae polyphenol extracting solution has sufficient nutrient components, ensured yield and stable quality, and is added with medium trace elements and functional substances to obtain the brown algae polyphenol trace element compound fertilizer with high utilization rate and wide application range, good fertilizer efficiency, enhanced crop stress resistance, yield increase and high quality.
Description
Technical Field
The invention belongs to the technical field of agricultural fertilizers, and particularly relates to a brown algae polyphenol trace element compound fertilizer and a preparation method thereof.
Background
China is a big agricultural country and agricultural productionHas higher dependence on chemical fertilizers. China consumes 35% of global chemical fertilizers in 7% of cultivated land in the world, and the total consumption amount of the chemical fertilizers and the application amount of the chemical fertilizers in unit area are at extremely high dangerous level. The internationally recognized safety upper limit of average application of the fertilizer per unit area is 225Kg/hm2(kilogram per square hectare), the Chinese reaches 434.3Kg/hm2The amount of the fertilizer applied in unit farmland area is 1.93 times of the upper limit of safety, and the fertilizer is applied in the second world. The use of a large amount of fertilizer increases the soil hardness and acidifies, reduces the utilization rate, and causes resource waste and environmental pollution. In recent years, the planting proportion of vegetables and fruits in China is increased year by year, however, the phenomenon that melons are not sweet and vegetables are not fragrant is caused by the fact that a large amount of traditional chemical fertilizers are used, so that the novel plant nutrition functional fertilizer needs to be developed, the investment of the chemical fertilizers is reduced, the absorption rate and the stress resistance of plants to nutrition are improved, the crop quality is improved, and the deep development of the seaweed functional fertilizer has very important significance for realizing agricultural sustainable development.
The microelements comprise boron, zinc, molybdenum, ferrum, manganese, copper and other nutrient elements. Although trace elements are required in plants in small amounts, their effect on the growth and development of plants is equally important as for macroelements. When certain trace elements are deficient, the growth and development of crops are obviously influenced, the yield is reduced, and the quality is reduced. On the other hand, excessive trace elements can poison crops, the yield and the quality are slightly affected, and the health of people and livestock is even threatened in serious cases. Therefore, the use amount and reasonable collocation of the trace elements are very important. With the continuous improvement of crop yield and the large-scale application of fertilizers, the application of trace element fertilizers is gradually urgent.
The seaweed fertilizer is characterized in that seaweed or seaweed extracts are added into the fertilizer, and the seaweed extracts greatly retain natural active ingredients of the seaweed, so that the seaweed fertilizer has the advantages of comprehensive nutrition, improvement of crop resistance, improvement of soil microecology, nature, no public nuisance and the like. Currently, some seaweed water-soluble fertilizers have appeared in the prior art, such as: chinese patent CN104945107A discloses a seaweed water-soluble fertilizer and an application method thereof, and the seaweed water-soluble fertilizer comprises the following components: 10-25 parts of seaweed extract, 0-100 parts of urea, 10-50 parts of ammonium dihydrogen phosphate, 10-140 parts of potassium nitrate, 1-80 parts of chelated calcium, 1-30 parts of chelated magnesium, 1-2 parts of chelated iron, 1-2 parts of chelated copper, 1-2 parts of chelated zinc, 1-2 parts of chelated manganese, 1-2 parts of polymeric boric acid and 0.5-3 parts of silicon dioxide; the seaweed water-soluble fertilizer is mainly prepared by simply compounding commercial seaweed extract and inorganic nutrient elements, and the seaweed extract has insufficient total nutrient content, so that the seaweed water-soluble fertilizer has poor effect, smaller application range of inorganic nutrient proportion, low utilization rate of inorganic nutrient and poor stress resistance of crops; moreover, the yield of the seaweed extract is limited, and the preparation method of the seaweed extract has great influence on the effect of the fertilizer, so that the supply amount of the seaweed water-soluble fertilizer is small, and the market demand cannot be met.
Disclosure of Invention
The invention aims to provide a brown algae polyphenol trace element compound fertilizer and a preparation method thereof, and aims to solve the problems that in the prior art, a seaweed water-soluble fertilizer is poor in fertilizer effect, reduces stress resistance of crops and cannot meet market demands due to insufficient total nutrient, low yield and unstable quality of seaweed extract.
In order to solve the technical problems, the invention is mainly realized by the following technical scheme:
in one aspect, the brown algae polyphenol trace element compound fertilizer comprises the following components in parts by weight: 35-70 parts of brown algae polyphenol extracting solution, 800.1-5 parts of tween, 10-20 parts of urea, 10-20 parts of monopotassium phosphate, 0.1-1 part of EDTA-Cu, 0.1-1 part of EDTA-Fe, 0.1-1 part of EDTA-Mn, 0.1-1 part of EDTA-Mg, 0.1-2 parts of boric acid, 0.01-0.1 part of ammonium molybdate heptahydrate and 1-20 parts of mannitol; the preparation method of the brown algae polyphenol extracting solution comprises the following steps: 1) taking brown algae, cleaning, airing, grinding into powder, adding water for dilution, wherein the amount of the water is 10-20 times of the dry weight of the powder brown algae, and carrying out soaking treatment for 20-30h to obtain brown algae slurry; 2) uniformly stirring the brown algae slurry obtained in the step 1), adding a biological enzyme complexing agent, wherein the dosage of the biological enzyme complexing agent is 0.2-0.5% of the dry weight of the powdery brown algae, the enzyme activity of the biological enzyme complexing agent is 10000-15000U/g, and preserving heat for 4-10h at the temperature of 20-60 ℃ to finish biological enzymolysis to obtain an enzymolysis solution; 3) heating the enzymolysis liquid obtained in the step 2) to 60-80 ℃, stirring for 20-40min, leaching with hot water, centrifuging, and taking the supernatant to obtain a brown algae polyphenol crude extract; 4) adding a potassium sulfite solution and magnesium sulfate into the brown algae polyphenol crude extract obtained in the step 3), wherein the mass concentration of the potassium sulfite solution is 40-55%, the adding amount of the potassium sulfite is 2-4% of the dry weight of the powdery brown algae, the adding amount of the magnesium sulfate is 10-20% of the dry weight of the powdery brown algae, heating to 60-80 ℃, adjusting the pH value to 6-7, settling for 1-3h, centrifuging, and removing the supernatant to obtain an intermediate product; 5) adding a sulfuric acid solution with the pH value of 4-5 into the intermediate product obtained in the step 4), wherein the dosage of the sulfuric acid solution is 10-15 times of the dry weight of the powdery brown algae, dissolving for 1-2h, and adjusting the pH value to 6-7 to obtain the brown algae polyphenol extracting solution.
The invention takes brown algae as raw material, carries out enzymolysis under the action of a biological enzyme complexing agent, and uses an ion precipitation method to enrich and purify active substances in the brown algae extract; the extraction method of the brown algae polyphenol extracting solution organically combines a biological enzyme degradation method and a chemical method, so that active ingredients in brown algae are retained to the maximum extent, the obtained brown algae polyphenol extracting solution is sufficient in nutrient components, the preparation method of the brown algae polyphenol extracting solution is simple, convenient to operate and easy to realize industrialization, the extraction rate of the brown algae polyphenol extracting solution is increased, the yield is ensured, the quality is stable, and the requirement of a brown algae polyphenol trace element compound fertilizer is met. According to the invention, the brown algae polyphenol extracting solution prepared by the method is added with a functional substance mannitol and matched with medium and trace elements such as nutrients urea, monopotassium phosphate, ammonium molybdate heptahydrate and copper, iron, manganese, magnesium, boric acid for crop growth, so that the brown algae polyphenol trace element compound fertilizer with high utilization rate and wide application range is obtained; the brown algae polyphenol trace element compound fertilizer has the advantages of fast crop absorption, high nutrient component utilization rate, enhanced fertilizer functionality, good fertilizer efficiency, maximum effect, enhanced crop stress resistance, improved crop quality and great promotion of crop yield increase.
In the invention, the brown algae polyphenol can regulate secondary metabolites in plants, is a unique defensive substance on a molecular level, has a good resistance effect on insect pests, can reduce feeding of plant-feeding animal pests to the plants, and is a basic substance for preventing the plant-feeding animal pests. The brown algae polyphenols can also affect the growth and reproduction of plant-eating animal pests, inhibit the growth of certain animal pests and reduce the survival rate of the animal pests. The brown algae polyphenol can improve and induce the expression of resistance genes, induce the formation of defense protein polyphenol oxidase or increase the activity of the defense protein polyphenol oxidase, and improve the disease resistance and damage resistance of plants; when plants are infected by pathogenic bacteria and wounded, polyphenol substances are quickly oxidized into quinone substances under the catalytic action of polyphenol oxidase to form brown pigment scabs to prevent scab infection and diffusion, callus formation is promoted, the plants can adapt to the external environment to play a role in plant self protection, the adverse environment of the plants to biotic and abiotic stresses such as high ultraviolet radiation, high light, low temperature, wound, malnutrition and pathogenic bacteria infection is improved, the insect resistance, disease resistance and stress resistance of the plants are improved, seed germination is promoted, the germination rate is improved, plant growth is promoted, the stress resistance of the plants is improved, fruit development is promoted, the yield and quality of fruits are improved, the utilization rate of the plants to soil nutrients is improved, and the like.
As a preferred embodiment, the compound biological enzyme preparation is any one or more of protease, cellulase, beta-glucosidase, chitinase, agarase, alginase, pectinase and chitinase. The composite biological enzyme preparation is a complex enzyme system of protease, cellulase, beta-glucosidase, chitinase, agarase, alginase, pectinase, chitinase and the like, gradually degrades brown algae, gradually releases active ingredients of brown algae polyphenol, alginic acid, polysaccharide, phytohormone and the like, retains the active ingredients in the brown algae to the maximum extent, and has sufficient nutrient components of the obtained brown algae polyphenol extracting solution.
In a preferred embodiment, the compound biological enzyme preparation is a mixture of protease, cellulase and agarase in a mass ratio of 2:1: 2. The invention adopts a compound enzyme system consisting of protease, cellulase and agarase, the components of the compound enzyme system interact and are mutually promoted, the enzymolysis is further enhanced, the enzymolysis speed is high, the enzymolysis effect is good, the functional groups of bioactive substances are not damaged in the enzymolysis process, the composition is simple, the price is low, the preparation is simple and convenient, and the degradation product also has the functions of promoting cell division and promoting root system growth in use.
In a preferred embodiment, the compound bio-enzyme preparation is a mixture of agarase, alginase and pectinase according to a mass ratio of 1:2: 2. According to the invention, a compound enzyme system consisting of agarase, alginase and pectinase is adopted, and under the action of the compound enzyme system, the brown algae extracting solution keeps active substances such as brown algae polyphenol, alginic acid and plant growth promoting factors, so that the content of the brown algae polyphenol and the content of effective active ingredients are improved; the macromolecular fragments in the seaweed cell contents can be hydrolyzed into micromolecular fragments which are easy to be absorbed by crops, and the degradation products have good effects on improving the quality of the crops, increasing the accumulation of dry vegetable substances and enhancing the stress resistance of fruit trees.
In a preferred embodiment, the compound biological enzyme preparation is a mixture of cellulase, beta-glucosidase, alginase and agarase according to a mass ratio of 2:1:1: 2. The invention adopts a compound enzyme system consisting of cellulase, beta-glucosidase, alginase and agarase, the enzymolysis of the compound enzyme system is strongest, under the action of the compound enzyme system, the brown algae extract furthest retains active substances such as brown algae polyphenol, alginic acid, plant growth promoting factors and the like, and the content of the brown algae polyphenol and the content of effective active ingredients are obviously improved; the four complex enzymes adopt proper proportion, release active substances in seaweed cells to the maximum extent, have good degradation effect on cell walls and macromolecular substances of different seaweeds, do not need to repeatedly add various single enzymes, simplify the operation process and improve the extraction efficiency.
As a preferred embodiment, the enzyme activity of the protease is 15000-. The enzymes used in the compound biological enzyme preparation of the invention are all commercial enzyme preparations, and the enzyme preparations have wide sources, easily obtained materials and convenient use.
As a preferred embodiment, the brown algae is any one or more of kelp, gulfweed, Ascophyllum nodosum and Undaria pinnatifida. According to the invention, the brown algae is used for preparing the extracting solution rich in endogenous active substances, the extracting solution is compounded with a specific medium trace element formula, and nutrient substances with specific functions such as mannitol and the like are added, so that the fertilizer is widely applicable to crops, is particularly suitable for growth of vegetables and fruits, can fully meet nutrient requirements and stress resistance of the crops, improves soil environment and improves crop quality.
In a preferred embodiment, the brown algae is a mixture of gulfweed, Ascophyllum nodosum and Undaria pinnatifida according to the mass ratio of 1:2: 1. Brown algae has no single cell, is a multicellular plant, and is the simplest branched filament consisting of single-row cells; the brown algae of the invention have various types and easily obtained materials, and are preferably extracted by adopting a mixture of gulfweed, Ascophyllum nodosum and Undaria pinnatifida, and the three brown algae are jointly extracted to obtain brown algae polyphenol, which has rich effective components and sufficient nutrient components; the three seaweeds are mixed according to a reasonable proportion for use, the extracted nutrients are more comprehensive, and the proportion of the released growth promoting substances meets the requirements of crops.
As a preferred embodiment, the mannitol has a density of 1.4 to 1.5g/cm3The purity of the mannitol is more than 98%. The mannitol is a product sold in the market, and the mannitol is abundant in source and easy to obtain; the invention also supplements mannitol which is a functional substance, enhances the mannitol content in the trace element fertilizer in the brown algae polyphenol, improves the fertilizer efficiency of the trace element fertilizer in the brown algae polyphenol, has scientific and reasonable inorganic nutrient matching and wide application range, improves the utilization rate of the inorganic nutrient and enhances the stress resistance of crops.
In another aspect, the invention provides a preparation method of a brown algae polyphenol medium trace element fertilizer, which comprises the following steps: 1) taking a brown algae polyphenol extracting solution, tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol for later use; 2) mixing the brown algae polyphenol extracting solution, Tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol at normal temperature, and stirring for 1-2 hours at the stirring speed of 100-200r/min to obtain the brown algae polyphenol trace element compound fertilizer.
The preparation method of the brown algae polyphenol medium trace element fertilizer mixes all components at normal temperature, and then controls the stirring speed to stir, so that all the components fully act; the brown algae polyphenol medium trace element fertilizer can be sprayed, flushed and irrigated, meets the development requirement of modern agriculture water and fertilizer integration, can protect the environment, can also fertilize the soil, and improves the crop quality.
Compared with the prior art, the invention has the beneficial effects that: the preparation method of the brown algae polyphenol extracting solution takes brown algae as a raw material, carries out enzymolysis under the action of a biological enzyme complexing agent, uses an ion precipitation method to enrich and purify active substances in a brown algae extract, organically combines a biological enzyme degradation method and a chemical method, furthest retains active ingredients in the brown algae, has sufficient nutrient components of the obtained brown algae polyphenol extracting solution, is simple and convenient to operate, is easy to realize industrialization, increases the extraction rate of the brown algae polyphenol extracting solution, ensures the yield, has stable quality and meets the requirements of a brown algae polyphenol trace element compound fertilizer. According to the invention, the brown algae polyphenol extracting solution prepared by the method is added with a functional substance mannitol and matched with medium and trace elements such as nutrients urea, monopotassium phosphate, ammonium molybdate heptahydrate and copper, iron, manganese, magnesium, boric acid for crop growth, so that the brown algae polyphenol trace element compound fertilizer with high utilization rate and wide application range is obtained; the brown algae polyphenol trace element compound fertilizer has the advantages of fast crop absorption, high nutrient component utilization rate, enhanced fertilizer functionality, good fertilizer efficiency, maximum effect, enhanced crop stress resistance, improved crop quality and great promotion of crop yield increase; the fertilizer can be sprayed, flushed and irrigated, is suitable for the development requirement of water and fertilizer integration of modern agriculture, can protect the environment, can also fertilize the soil, and improves the quality of crops.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention relates to a brown algae polyphenol trace element compound fertilizer which comprises the following components in parts by weight: 35-70 parts of brown algae polyphenol extracting solution, 800.1-5 parts of tween, 10-20 parts of urea, 10-20 parts of monopotassium phosphate, 0.1-1 part of EDTA-Cu, 0.1-1 part of EDTA-Fe, 0.1-1 part of EDTA-Mn, 0.1-1 part of EDTA-Mg, 0.1-2 parts of boric acid, 0.01-0.1 part of ammonium molybdate heptahydrate and 1-20 parts of mannitol; the preparation method of the brown algae polyphenol extracting solution comprises the following steps: 1) taking brown algae, cleaning, airing, grinding into powder, adding water for dilution, wherein the amount of the water is 10-20 times of the dry weight of the powder brown algae, and carrying out soaking treatment for 20-30h to obtain brown algae slurry; 2) uniformly stirring the brown algae slurry obtained in the step 1), adding a biological enzyme complexing agent, wherein the dosage of the biological enzyme complexing agent is 0.2-0.5% of the dry weight of the powdery brown algae, the enzyme activity of the biological enzyme complexing agent is 10000-15000U/g, and preserving heat for 4-10h at the temperature of 20-60 ℃ to finish biological enzymolysis to obtain an enzymolysis solution; 3) heating the enzymolysis liquid obtained in the step 2) to 60-80 ℃, stirring for 20-40min, leaching with hot water, centrifuging, and taking the supernatant to obtain a brown algae polyphenol crude extract; 4) adding a potassium sulfite solution and magnesium sulfate into the brown algae polyphenol crude extract obtained in the step 3), wherein the mass concentration of the potassium sulfite solution is 40-55%, the adding amount of the potassium sulfite is 2-4% of the dry weight of the powdery brown algae, the adding amount of the magnesium sulfate is 10-20% of the dry weight of the powdery brown algae, heating to 60-80 ℃, adjusting the pH value to 6-7, settling for 1-3h, centrifuging, and removing the supernatant to obtain an intermediate product; 5) adding a sulfuric acid solution with the pH value of 4-5 into the intermediate product obtained in the step 4), wherein the dosage of the sulfuric acid solution is 10-15 times of the dry weight of the powdery brown algae, dissolving for 1-2h, and adjusting the pH value to 6-7 to obtain the brown algae polyphenol extracting solution.
Preferably, the compound bio-enzyme preparation is any one or more of protease, cellulase, beta-glucosidase, chitinase, agarase, alginase, pectinase and chitinase.
Further, the compound biological enzyme preparation is a mixture of protease, cellulase and agarase according to the mass ratio of 2:1: 2.
Still further, the compound bio-enzyme preparation is a mixture of agarase, alginase and pectinase according to the mass ratio of 1:2: 2.
Furthermore, the compound biological enzyme preparation is a mixture consisting of cellulase, beta-glucosidase, alginase and agarase according to the mass ratio of 2:1:1: 2.
Preferably, the enzyme activity of the protease is 15000-25000U/g, the enzyme activity of the cellulase is 1500-2500U/g, the enzyme activity of the beta-glucosidase is 5000-15000U/g, the enzyme activity of the chitinase is 5000-15000U/g, the enzyme activity of the agarase is 4000-6000U/g, the enzyme activity of the alginase is 5000-15000U/g, the enzyme activity of the pectinase is 4000-6000U/g, and the enzyme activity of the chitinase is 3000-5000U/g.
Specifically, the brown algae is one or more of kelp, gulfweed, Ascophyllum nodosum and Undaria pinnatifida.
More specifically, the brown algae is a mixture of gulfweed, Ascophyllum nodosum and Undaria pinnatifida according to the mass ratio of 1:2: 1.
More preferably, the mannitol has a density of 1.4-1.5g/cm3The purity of the mannitol is more than 98%.
The invention relates to a preparation method of a brown algae polyphenol medium trace element fertilizer, which comprises the following steps: 1) taking a brown algae polyphenol extracting solution, tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol for later use; 2) mixing the brown algae polyphenol extracting solution, Tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol at normal temperature, and stirring for 1-2 hours at the stirring speed of 100-200r/min to obtain the brown algae polyphenol trace element compound fertilizer.
Example one
The invention relates to a preparation method of a brown algae polyphenol trace element compound fertilizer, which comprises the following steps:
preparation of S1 brown algae polyphenol extract
1) Taking Undaria pinnatifida, cleaning, drying in the air, grinding into powder, taking 10Kg of the powdery Undaria pinnatifida, adding 200Kg of water, soaking, and treating for 24h to obtain Undaria pinnatifida pulp;
2) uniformly stirring the undaria pinnatifida pulp obtained in the step 1), adding the mixture into a constant temperature tank, adjusting the pH value to 6.2, adding 0.008Kg of agarase and 0.008Kg of cellulase to perform primary reaction, controlling the reaction temperature to be 35 ℃, reacting for 8 hours, then adjusting the temperature to 55 ℃, adjusting the pH value to 6.8, adding 0.004Kg of alginase and 0.004Kg of beta-glucosidase, and continuing enzymolysis for 8 hours to obtain an enzymolysis solution; the enzyme activity of the cellulase is 2000U/g, the enzyme activity of the beta-glucosidase is 10000U/g, the enzyme activity of the agarase is 5000U/g, and the enzyme activity of the alginase is 10000U/g;
3) heating the enzymolysis liquid obtained in the step 2) to 70 ℃, stirring for 30min, leaching with hot water, centrifuging, and taking the supernatant to obtain a crude undaria pinnatifida polyphenol extracting solution;
4) adding 0.6Kg of potassium sulfite solution and 1.5Kg of magnesium sulfate into the crude undaria pinnatifida polyphenol extracting solution obtained in the step 3), wherein the mass concentration of the potassium sulfite solution is 50%, heating to 70 ℃, adding potassium bicarbonate to adjust the pH value to be 6.0, settling for 2 hours, centrifuging, and removing supernatant to obtain an intermediate product;
5) adding a sulfuric acid solution with the pH value of 4.5 into the intermediate product obtained in the step 4), dissolving the sulfuric acid solution for 1 hour by using 100Kg, and adjusting the pH value to 6.5 by using potassium hydroxide to obtain an undaria pinnatifida polyphenol extracting solution;
preparation of S2 compound fertilizer
6) 56.38Kg of undaria pinnatifida polyphenol extracting solution obtained in the step S1, 800.5 Kg of tween, 15Kg of urea, 15Kg of monopotassium phosphate, 0.5Kg of EDTA-Cu, 0.5Kg of EDTA-Fe, 0.1Kg of EDTA-Mn, 1Kg of EDTA-Mg, 1Kg of boric acid, 0.02Kg of ammonium molybdate heptahydrate and 10Kg of mannitol are taken for later use;
7) mixing the undaria pinnatifida polyphenol extracting solution, tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol at normal temperature, and stirring for 2 hours at the stirring speed of 150r/min to obtain the brown algae polyphenol trace element compound fertilizer.
Example two
The invention relates to a preparation method of a brown algae polyphenol trace element compound fertilizer, which comprises the following steps:
preparation of S1 brown algae polyphenol extract
1) Taking Ascophyllum nodosum and gulfweed, mixing the Ascophyllum nodosum source and the gulfweed according to the weight ratio of 1:1, cleaning, airing, and grinding into powder to obtain powdery brown algae; taking 20Kg of powdery brown algae, adding 200Kg of water, soaking, and treating for 30h to obtain brown algae slurry;
2) uniformly stirring the brown algae slurry obtained in the step 1), adding the brown algae slurry into a constant-temperature tank, adjusting the pH value to 6.5, adding 0.008Kg of agarase, 0.016Kg of alginase and 0.016Kg of pectinase to perform primary reaction, controlling the reaction temperature to be 20 ℃, and reacting for 10 hours to obtain an enzymatic hydrolysate; the enzyme activity of the agarase is 5000U/g, the enzyme activity of the alginase is 10000U/g, and the enzyme activity of the pectinase is 5000U/g;
3) heating the enzymolysis liquid obtained in the step 2) to 60 ℃, stirring for 40min, leaching with hot water, centrifuging, and taking the supernatant to obtain a brown algae polyphenol crude extract;
4) adding 0.8Kg of potassium sulfite solution and 2.0Kg of magnesium sulfate into the brown algae polyphenol crude extract obtained in the step 3), wherein the mass concentration of the potassium sulfite solution is 50%, heating to 80 ℃, adding potassium bicarbonate to adjust the pH value to 7.0, settling for 1h, centrifuging, and removing supernatant to obtain an intermediate product;
5) adding a sulfuric acid solution with the pH value of 4.0 into the intermediate product obtained in the step 4), dissolving the sulfuric acid solution for 2 hours at the dosage of 300Kg, and adjusting the pH value to 6.0 by adopting potassium hydroxide to obtain a brown algae polyphenol extracting solution;
preparation of S2 compound fertilizer
6) Taking 66.38Kg of brown algae polyphenol extracting solution obtained in the step S1, 800.5 Kg of tween-urea, 10Kg of monopotassium phosphate, 0.5Kg of EDTA-Cu, 0.5Kg of EDTA-Fe, 0.1Kg of EDTA-Mn, 1Kg of EDTA-Mg, 1Kg of boric acid, 0.02Kg of ammonium molybdate heptahydrate and 10Kg of mannitol for later use;
7) mixing the brown algae polyphenol extracting solution, tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol at normal temperature, and stirring for 2 hours at the stirring speed of 100r/min to obtain the brown algae polyphenol trace element compound fertilizer.
EXAMPLE III
The invention relates to a preparation method of a brown algae polyphenol trace element compound fertilizer, which comprises the following steps:
preparation of S1 brown algae polyphenol extract
1) Taking kelp and undaria pinnatifida, mixing the kelp and the undaria pinnatifida according to the weight ratio of 1:2, cleaning, airing, and grinding into powder to obtain powdery brown algae; taking 10Kg of powdery brown algae, adding 200Kg of water, soaking, and treating for 20h to obtain brown algae slurry;
2) uniformly stirring the brown algae slurry obtained in the step 1), adding the brown algae slurry into a constant-temperature tank, adjusting the pH value to 6.8, adding 0.02Kg of agarase and 0.01Kg of cellulase to perform primary reaction, controlling the reaction temperature to be 60 ℃, reacting for 4 hours, then adjusting the temperature to 25 ℃, adding 0.02Kg of protease, adjusting the pH value to 5.5, and continuing enzymolysis for 4 hours to obtain an enzymolysis solution; the enzyme activity of the protease is 20000U/g, the enzyme activity of the cellulase is 2000U/g, and the enzyme activity of the agarase is 5000U/g;
3) heating the enzymolysis liquid obtained in the step 2) to 80 ℃, stirring for 20min, leaching with hot water, centrifuging, and taking the supernatant to obtain a brown algae polyphenol crude extract;
4) adding 0.8Kg of potassium sulfite solution and 2Kg of magnesium sulfate into the brown algae polyphenol crude extract obtained in the step 3), wherein the mass concentration of the potassium sulfite solution is 50%, heating to 80 ℃, adding potassium bicarbonate to adjust the pH value to be 6.5, settling for 3h, centrifuging, and removing supernatant to obtain an intermediate product;
5) adding a sulfuric acid solution with the pH value of 4.5 into the intermediate product obtained in the step 4), dissolving the sulfuric acid solution for 1 hour by using 100Kg, and adjusting the pH value to 6.5 by using potassium hydroxide to obtain an undaria pinnatifida polyphenol extracting solution;
preparation of S2 compound fertilizer
6) 51.38Kg of brown algae polyphenol extracting solution obtained in the step S1, 800.5 Kg of tween, 10Kg of urea, 20Kg of monopotassium phosphate, 0.25Kg of EDTA-Cu, 0.25Kg of EDTA-Fe, 0.1Kg of EDTA-Mn, 1Kg of EDTA-Mg, 1.5Kg of boric acid, 0.02Kg of ammonium molybdate heptahydrate and 15Kg of mannitol are taken for later use;
7) mixing the brown algae polyphenol extracting solution, tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol at normal temperature, and stirring for 1h at the stirring speed of 200r/min to obtain the brown algae polyphenol trace element compound fertilizer.
Example four
The invention relates to a preparation method of a brown algae polyphenol trace element compound fertilizer, which comprises the following steps:
preparation of S1 brown algae polyphenol extract
1) Taking gulfweed, Ascophyllum nodosum and Undaria pinnatifida, mixing the gulfweed, Ascophyllum nodosum and Undaria pinnatifida according to the weight ratio of 1:2:1, cleaning, airing, and grinding into powder to obtain powdery brown algae; taking 12.5Kg of powdery brown algae, adding 200Kg of water, soaking, and treating for 24h to obtain brown algae slurry;
2) uniformly stirring the brown algae slurry obtained in the step 1), adding the brown algae slurry into a constant-temperature tank, adjusting the pH value to 6.8, adding 0.02Kg of agarase and 0.01Kg of cellulase to perform primary reaction, controlling the reaction temperature to be 45 ℃, reacting for 4 hours, then adjusting the temperature to 25 ℃, adding 0.02Kg of protease, adjusting the pH value to 5.5, and continuing enzymolysis for 4 hours to obtain an enzymolysis solution; the enzyme activity of the protease is 15000U/g, the enzyme activity of the cellulase is 2500U/g, and the enzyme activity of the agarase is 4000U/g;
3) heating the enzymolysis liquid obtained in the step 2) to 70 ℃, stirring for 30min, leaching with hot water, centrifuging, and taking the supernatant to obtain a brown algae polyphenol crude extract;
4) adding 0.9Kg of potassium sulfite solution and 1.875Kg of magnesium sulfate into the brown algae polyphenol crude extract obtained in the step 3), wherein the mass concentration of the potassium sulfite solution is 40%, heating to 70 ℃, adding potassium bicarbonate to adjust the pH value to be 6.0, settling for 1h, centrifuging, and removing supernatant to obtain an intermediate product;
5) adding a sulfuric acid solution with the pH value of 4.0 into the intermediate product obtained in the step 4), dissolving the sulfuric acid solution for 1 hour by using the amount of 150Kg, and adjusting the pH value to 6.0 by using potassium hydroxide to obtain an undaria pinnatifida polyphenol extracting solution;
preparation of S2 compound fertilizer
6) Taking 35Kg of brown algae polyphenol extracting solution obtained in the step S1, 800.1 Kg of tween, 10Kg of urea, 20Kg of monopotassium phosphate, 0.1Kg of EDTA-Cu, 0.1Kg of EDTA-Fe, 0.1Kg of EDTA-Mn, 0.1Kg of EDTA-Mg, 0.1Kg of boric acid, 0.01Kg of ammonium molybdate heptahydrate and 1Kg of mannitol for later use;
7) mixing the brown algae polyphenol extracting solution, tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol at normal temperature, and stirring for 1h at the stirring speed of 200r/min to obtain the brown algae polyphenol trace element compound fertilizer.
EXAMPLE five
The invention relates to a preparation method of a brown algae polyphenol trace element compound fertilizer, which comprises the following steps:
preparation of S1 brown algae polyphenol extract
1) Taking kelp and undaria pinnatifida, mixing the kelp and the undaria pinnatifida according to the weight ratio of 1:2, cleaning, airing, and grinding into powder to obtain powdery brown algae; taking 10Kg of powdery brown algae, adding 200Kg of water, soaking, and treating for 24h to obtain brown algae slurry;
2) uniformly stirring the brown algae slurry obtained in the step 1), adding the brown algae slurry into a constant temperature tank, adjusting the pH value to 6.0, adding 0.008Kg of agarase and 0.008Kg of cellulase to perform primary reaction, controlling the reaction temperature to be 35 ℃, reacting for 8 hours, then adjusting the temperature to 55 ℃, adjusting the pH value to 6.8, adding 0.004Kg of alginase and 0.004Kg of beta-glucosidase, and continuing enzymolysis for 8 hours to obtain an enzymolysis solution; the enzyme activity of the cellulase is 2500U/g, the enzyme activity of the beta-glucosidase is 5000U/g, the enzyme activity of the agarase is 6000U/g, and the enzyme activity of the alginase is 5000U/g;
3) heating the enzymolysis liquid obtained in the step 2) to 70 ℃, stirring for 30min, leaching with hot water, centrifuging, and taking the supernatant to obtain a brown algae polyphenol crude extract;
4) adding 0.55Kg of potassium sulfite solution and 1.5Kg of magnesium sulfate into the brown algae polyphenol crude extract obtained in the step 3), wherein the mass concentration of the potassium sulfite solution is 55%, heating to 70 ℃, adding potassium bicarbonate to adjust the pH value to 7.0, settling for 1h, centrifuging, and removing supernatant to obtain an intermediate product;
5) adding a sulfuric acid solution with the pH value of 5 into the intermediate product obtained in the step 4), dissolving the sulfuric acid solution for 1.5h at the dosage of 150Kg, and adjusting the pH value to 7.0 by adopting potassium hydroxide to obtain an undaria pinnatifida polyphenol extracting solution;
preparation of S2 compound fertilizer
6) Taking 70Kg of brown algae polyphenol extracting solution obtained in the step S1, 20Kg of tween-805, 20Kg of urea, 20Kg of monopotassium phosphate, 1Kg of EDTA-Cu, 1Kg of EDTA-Fe, 1Kg of EDTA-Mn, 1Kg of EDTA-Mg1Kg, 2Kg of boric acid, 0.1Kg of ammonium molybdate heptahydrate and 20Kg of mannitol for later use;
7) mixing the brown algae polyphenol extracting solution, tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol at normal temperature, and stirring for 1h at the stirring speed of 200r/min to obtain the brown algae polyphenol trace element compound fertilizer.
Experiment 1
And selecting rape which grows uniformly and robustly and is divided into seven experimental fields, and respectively applying the brown alga polyphenol trace element compound fertilizer prepared in the first to fifth embodiments, a clear water blank sample and a certain commercially available alga water-soluble fertilizer control sample.
The fertilizing method comprises the following steps: irrigating roots, namely diluting the obtained compound fertilizer, blank samples and control samples by 500 times, irrigating roots for the first time in the seedling stage of the rape, and irrigating roots for the second time 25 days before the rape is mature; spraying, namely diluting the obtained compound fertilizer, blank samples and control samples by 800 times, starting spraying 15 days after field planting of rape, spraying the diluted compound fertilizer, blank samples and control samples on the reverse front side of rape leaves, spraying the diluted compound fertilizer, blank samples and control samples once every 20 days for 3 times in total, and spraying the diluted compound fertilizer, blank samples and control samples 3 times in total at intervals of 20 days; each treatment was repeated 3 times, with 50 oilseed rape in one repeat region.
And (3) statistical experiments: 30 rapes are selected in each repetitive area, young and tender leaves of the same parts of the rapes are picked up 40 days after planting, and the SPAD value of the leaves is measured by using a SPAD502 chlorophyll tester produced by Konikamenada. After the mature goods are received, the mass is measured by a balance respectively at room temperature for four times, and the average value is taken. The results are shown in Table 1.
In addition, the disease and insect pest conditions of the rapes in the planting process are recorded and measured by adopting disease index, the disease index is a comprehensive index which comprehensively considers the morbidity and the severity, and when the severity is represented by a grading representative value, the disease index DI is 100 multiplied by Sigma (the number of plants or organs multiplied by the disease level of each grade)/(the investigation total number or the number of organs multiplied by the highest disease level); when severity is expressed as a percentage, the disease index is incidence x severity x 100; incidence is the percentage of the total number of plants or plant organs (leaves, roots, stems, fruits, seeds, etc.) in the disease to be investigated, and is used to indicate the prevalence of the disease; disease severity represents the ratio of the diseased area of a plant or organ, for example: the ratio of the lesion area on the leaves to the total area of the leaves; the DI value at which the onset was the most severe was 100, and the total absence was 0, and the results are shown in Table 1.
Finally, selecting 11 advanced technical experts in the rape planting field, and respectively evaluating the quality of the seven samples, wherein the quality evaluation refers to the rape and the quality shown after the rape is processed, the quality indexes comprise the shape, color, uniformity, smell and taste of the rape, a blind evaluation mode is adopted, the results are recorded by a scoring method, the total score is 10, the worst score is 0, and the experimental results are listed in a table 1.
TABLE 1 rape planting test results
| Fertilizer name | SPDA value | Average single weight (g) | Condition of disease and pest | Quality of product |
| Example one | 47.23 | 68 | 22.5 | 9 minutes |
| Example two | 45.92 | 56 | 32.4 | 8 is divided into |
| EXAMPLE III | 45.04 | 61 | 34.1 | 8 is divided into |
| Example four | 45.64 | 62 | 26.8 | 9 minutes |
| EXAMPLE five | 46.62 | 65 | 25.5 | 9 minutes |
| Blank sample | 43.70 | 46 | 62.7 | 5 points of |
| Control sample | 44.21 | 49 | 54.1 | 7 points of |
As can be seen from the table 1, the application of the brown algae polyphenol trace element compound fertilizer provided by the invention obviously increases the SPDA value of rape seedling stage leaves, improves the chlorophyll content of rape, increases the photosynthesis of rape, and thus obviously improves the yield of rape. Meanwhile, the plant diseases and insect pests in the growth process of the rape are reduced, and the quality of the rape is improved.
Experiment 2
Selecting celery which grows well and strongly and is divided into seven experimental fields, and respectively applying the brown algae polyphenol trace element compound fertilizer prepared in the first to fifth embodiments, a clear water blank sample and a certain commercially available seaweed water-soluble fertilizer control sample.
The fertilizing method comprises the following steps: root irrigation, namely diluting the obtained compound fertilizer, blank sample and control sample by 500 times, performing first root irrigation in the seedling stage of celery, and performing second root irrigation 25 days before celery is mature; spraying, namely diluting the obtained compound fertilizer, blank sample and control sample by 800 times, starting spraying 15 days after celery field planting, spraying on the reverse front side of celery leaves, spraying once every 20 days for 3 times, and spraying 3 times at intervals of 20 days; each treatment was repeated 3 times, with 50 celery in one repeat area.
And (3) statistical experiments: selecting 30 celery in each repeating area, picking young leaves at the same part of the celery 40 days after planting, and measuring the SPAD value of the leaves by using a SPAD502 chlorophyll tester produced by Konika minolta. After the mature goods are received, the mass is measured by a balance respectively at room temperature for four times, and the average value is taken. The results of the experiment are shown in table 2.
In addition, the disease and insect pest conditions of celery in the planting process are recorded, the disease condition index is measured by adopting a disease condition index, the disease condition index is a comprehensive index which comprehensively considers the disease incidence and the severity, and when the severity is represented by a grading representative value, the disease condition index DI is 100 multiplied by Sigma (the number of plants or organs multiplied by the disease incidence level)/(the investigation total number or the number of organs multiplied by the highest disease incidence level); when severity is expressed as a percentage, the disease index is incidence x severity x 100; incidence is the percentage of the total number of plants or plant organs (leaves, roots, stems, fruits, seeds, etc.) in the disease to be investigated, and is used to indicate the prevalence of the disease; disease severity represents the ratio of the diseased area of a plant or organ, for example: the ratio of the lesion area on the leaves to the total area of the leaves; the DI value at the most severe onset was 100, and the total disease-free was 0, and the results are shown in Table 2.
And finally, selecting 11 advanced technologists in the field of celery planting, and respectively evaluating the quality of the seven samples, wherein the quality evaluation refers to the appearance quality and the internal quality of celery, the quality indexes comprise the length, the thickness, the color and luster of leaves, the palatability, the taste and the brittleness of the celery, a blind evaluation mode is adopted, the result is recorded by a scoring method, the total score is 10, the worst score is 0, and the experimental result is shown in table 2.
Table 2 celery planting test results
| Fertilizer name | SPDA value | Average single weight (g) | Condition of disease and pest | Quality of product |
| Example one | 14.34 | 182 | Stage 18.9 | 9 minutes |
| Example two | 13.61 | 176 | Stage 26.4 | 8 is divided into |
| EXAMPLE III | 12.10 | 169 | Stage 33.6 | 7 points of |
| Example four | 14.63 | 179 | Stage 28.1 | 8 is divided into |
| EXAMPLE five | 13.36 | 171 | 35.2 stage | 7 points of |
| Blank sample | 10.48 | 147 | Level 62.1 | 5 points of |
| Control sample | 11.87 | 160 | Stage 53.8 | 6 minutes |
As can be seen from the table 2, the application of the brown algae polyphenol trace element compound fertilizer provided by the invention obviously increases the SPDA value of celery seedling stage leaves, improves the chlorophyll content of celery, increases the photosynthesis of celery, and thus obviously improves the yield of celery. Meanwhile, the pest and disease damage in the growth process of the celery is reduced, and the quality of the celery is improved.
Experiment 3
Selecting cucumbers with uniform growth vigor and strong growth, dividing the cucumbers into seven experimental fields, and respectively applying the brown alga polyphenol trace element compound fertilizer prepared in the first to fifth embodiments, a clear water blank sample and a certain commercially available alga water-soluble fertilizer control sample.
The fertilizing method comprises the following steps: root irrigation, namely diluting the obtained compound fertilizer, the blank sample and the control sample by 500 times, performing first root irrigation in the seedling stage of the cucumbers, and performing second root irrigation after the cucumbers bear fruits and 5 days later; spraying, namely diluting the obtained compound fertilizer, blank samples and control samples by 800 times, starting spraying from 10 days after the cucumber fruits are set, spraying on the reverse front side of cucumber leaves, spraying once every 20 days for 3 times, and spraying 3 times at intervals of 20 days; each treatment was repeated 3 times, with 30 cucumbers being a repeat region.
And (3) statistical experiments: selecting 30 mature cucumbers in each repetition region, picking young leaves at the same parts of the cucumbers 40 days after planting, and measuring the SPAD value of the leaves by using a SPAD502 chlorophyll determinator produced by Konikamenada; after harvest, the mass was measured four times at room temperature using a balance, and the results are shown in table 3.
In addition, the disease and insect pest conditions of the cucumbers in the planting process are recorded and measured by using disease index, wherein the disease index is a comprehensive index which comprehensively considers disease incidence and severity, and when the severity is represented by a grading representative value, the disease index DI is 100 multiplied by (the number of plants or organs at each grade multiplied by the disease grade)/(the investigation total number or the number of organs multiplied by the highest disease grade); when severity is expressed as a percentage, the disease index is incidence x severity x 100; incidence is the percentage of the total number of plants or plant organs (leaves, roots, stems, fruits, seeds, etc.) in the disease to be investigated, and is used to indicate the prevalence of the disease; disease severity represents the ratio of the diseased area of a plant or organ, for example: the ratio of the lesion area on the leaves to the total area of the leaves; the DI value at the most severe onset was 100, and the total disease-free was 0, and the results are shown in Table 3.
And finally, selecting 11 advanced technical experts in the field of cucumber planting, and respectively evaluating the quality of the seven samples, wherein the quality evaluation refers to the appearance quality and the internal quality of the cucumber, the quality indexes comprise the shape, the color, the size, the defects, the flavor, the hardness, the toughness, the compactness and the bitterness of the cucumber, the blind evaluation mode is adopted, the result is recorded by a grading method, the total score is 10, the worst score is 0, and the experimental result is shown in table 3.
TABLE 3 cucumber planting test results
| Fertilizer name | SPDA value | Average single cucumber weight (g) | Condition of disease and pest | Quality of product |
| Example one | 38 | 240 | Stage 18.9 | 9 minutes |
| Example two | 31 | 231 | Stage 26.5 | 8 is divided into |
| EXAMPLE III | 29 | 228 | Level 37.1 | 7 points of |
| Example four | 30 | 232 | Stage 34.9 | 7 points of |
| EXAMPLE five | 32 | 241 | Stage 19.2 | 8 is divided into |
| Blank sample | 25 | 192 | Grade 63.5 | 5 points of |
| Control sample | 27 | 240 | Grade 54.7 | 6 minutes |
As can be seen from Table 3, the application of the brown algae polyphenol trace element compound fertilizer provided by the invention obviously increases the SPDA value of leaves in the seedling stage of cucumbers, improves the chlorophyll content of the cucumbers, increases the photosynthesis of the cucumbers, and thus obviously improves the yield of the cucumbers. Meanwhile, the plant diseases and insect pests in the growth process of the cucumber are reduced, and the quality of the cucumber is improved.
Therefore, compared with the prior art, the invention has the beneficial effects that: the preparation method of the brown algae polyphenol extracting solution takes brown algae as a raw material, carries out enzymolysis under the action of a biological enzyme complexing agent, uses an ion precipitation method to enrich and purify active substances in a brown algae extract, organically combines a biological enzyme degradation method and a chemical method, furthest retains active ingredients in the brown algae, has sufficient nutrient components of the obtained brown algae polyphenol extracting solution, is simple and convenient to operate, is easy to realize industrialization, increases the extraction rate of the brown algae polyphenol extracting solution, ensures the yield, has stable quality and meets the requirements of a brown algae polyphenol trace element compound fertilizer. According to the invention, the brown algae polyphenol extracting solution prepared by the method is added with a functional substance mannitol and matched with medium and trace elements such as nutrients urea, monopotassium phosphate, ammonium molybdate heptahydrate and copper, iron, manganese, magnesium, boric acid for crop growth, so that the brown algae polyphenol trace element compound fertilizer with high utilization rate and wide application range is obtained; the brown algae polyphenol trace element compound fertilizer has the advantages of fast crop absorption, high nutrient component utilization rate, enhanced fertilizer functionality, good fertilizer efficiency, maximum effect, enhanced crop stress resistance, improved crop quality and great promotion of crop yield increase; the fertilizer can be sprayed, flushed and irrigated, is suitable for the development requirement of water and fertilizer integration of modern agriculture, can protect the environment, can also fertilize the soil, and improves the quality of crops.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The brown algae polyphenol trace element compound fertilizer is characterized by comprising the following components in parts by weight:
35-70 parts of brown algae polyphenol extracting solution, 800.1-5 parts of tween, 10-20 parts of urea, 10-20 parts of monopotassium phosphate, 0.1-1 part of EDTA-Cu, 0.1-1 part of EDTA-Fe, 0.1-1 part of EDTA-Mn, 0.1-1 part of EDTA-Mg, 0.1-2 parts of boric acid, 0.01-0.1 part of ammonium molybdate heptahydrate and 1-20 parts of mannitol;
the preparation method of the brown algae polyphenol extracting solution comprises the following steps:
1) cleaning brown algae, air drying, grinding into powder, adding water for soaking, wherein the amount of water is 10-20 times of the dry weight of the powdery brown algae, and the soaking time is 20-30h, so as to obtain brown algae slurry;
2) stirring the brown algae slurry obtained in the step 1) uniformly, adding a biological enzyme complexing agent for enzymolysis, wherein the dosage of the biological enzyme complexing agent is 0.2-0.5% of the dry weight of the powdery brown algae, the enzyme activity of the biological enzyme complexing agent is 10000-15000U/g, and preserving heat for 4-10h at the temperature of 20-60 ℃ to obtain an enzymolysis solution;
3) heating the enzymolysis liquid obtained in the step 2) to 60-80 ℃, stirring for 20-40min, leaching with hot water, centrifuging, and taking the supernatant to obtain a brown algae polyphenol crude extract;
4) adding a potassium sulfite solution and magnesium sulfate into the brown algae polyphenol crude extract obtained in the step 3), wherein the mass concentration of the potassium sulfite solution is 40-55%, the adding amount of the potassium sulfite is 2-4% of the dry weight of the powdery brown algae, the adding amount of the magnesium sulfate is 10-20% of the dry weight of the powdery brown algae, heating to 60-80 ℃, adjusting the pH value to 6-7, settling for 1-3h, and centrifuging to obtain an intermediate product;
5) adding a sulfuric acid solution with the pH value of 4-5 into the intermediate product obtained in the step 4), wherein the dosage of the sulfuric acid solution is 10-15 times of the dry weight of the powdery brown algae, dissolving for 1-2h, and adjusting the pH value to 6-7 to obtain the brown algae polyphenol extracting solution.
2. The brown algae polyphenol trace element compound fertilizer as claimed in claim 1, which is characterized in that:
the compound biological enzyme preparation is any one or more of protease, cellulase, beta-glucosidase, chitinase, agarase, alginase, pectinase and chitinase.
3. The brown algae polyphenol trace element compound fertilizer as claimed in claim 2, which is characterized in that:
the compound biological enzyme preparation is a mixture of protease, cellulase and agarase according to the mass ratio of 2:1: 2.
4. The brown algae polyphenol trace element compound fertilizer as claimed in claim 2, which is characterized in that:
the compound biological enzyme preparation is a mixture of agarase, alginase and pectinase according to the mass ratio of 1:2: 2.
5. The brown algae polyphenol trace element compound fertilizer as claimed in claim 2, which is characterized in that:
the compound biological enzyme preparation is a mixture consisting of cellulase, beta-glucosidase, alginase and agarase according to the mass ratio of 2:1:1: 2.
6. The brown algae polyphenol trace element compound fertilizer as claimed in claim 2, which is characterized in that:
the enzyme activity of the protease is 15000-.
7. The brown algae polyphenol trace element compound fertilizer as claimed in claim 1, which is characterized in that:
the brown algae is one or more of herba Zosterae Marinae, Sargassum, Ascophyllum nodosum, and Undaria Pinnatifida.
8. The brown algae polyphenol trace element compound fertilizer as claimed in claim 7, which is characterized in that:
the brown algae is a mixture of gulfweed, Ascophyllum nodosum and Undaria pinnatifida according to a mass ratio of 1:2: 1.
9. The brown algae polyphenol trace element compound fertilizer as claimed in claim 1, which is characterized in that:
the density of the mannitol is 1.4-1.5g/cm3The purity of the mannitol is more than 98%.
10. The preparation method of the brown algae polyphenol trace element compound fertilizer according to any one of claims 1 to 9, comprising the following steps:
1) taking a brown algae polyphenol extracting solution, tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol for later use;
2) mixing the brown algae polyphenol extracting solution, Tween 80, urea, monopotassium phosphate, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Mg, boric acid, ammonium molybdate heptahydrate and mannitol at normal temperature, and stirring for 1-2 hours at the stirring speed of 100-200r/min to obtain the brown algae polyphenol trace element compound fertilizer.
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| CN113511940A (en) * | 2021-07-26 | 2021-10-19 | 武汉博润科技有限公司 | Compound fertilizer and preparation method thereof |
| CN116082087A (en) * | 2022-12-28 | 2023-05-09 | 青岛苏贝尔作物营养有限公司 | Brown algae polyphenol-iron complex for preventing and treating plant yellowing and preparation method thereof |
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| CN108083880A (en) * | 2017-12-27 | 2018-05-29 | 中化（烟台）作物营养有限公司 | A kind of organic water-soluble fertilizer containing alginic acid and preparation method thereof |
| CN110105121A (en) * | 2019-04-16 | 2019-08-09 | 广西鼎好农业科技有限公司 | A kind of sargassum method of comprehensive utilization |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108083880A (en) * | 2017-12-27 | 2018-05-29 | 中化（烟台）作物营养有限公司 | A kind of organic water-soluble fertilizer containing alginic acid and preparation method thereof |
| CN110105121A (en) * | 2019-04-16 | 2019-08-09 | 广西鼎好农业科技有限公司 | A kind of sargassum method of comprehensive utilization |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113511940A (en) * | 2021-07-26 | 2021-10-19 | 武汉博润科技有限公司 | Compound fertilizer and preparation method thereof |
| CN116082087A (en) * | 2022-12-28 | 2023-05-09 | 青岛苏贝尔作物营养有限公司 | Brown algae polyphenol-iron complex for preventing and treating plant yellowing and preparation method thereof |
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Application publication date: 20201110 |