CN111903973A - A health food with lung nourishing effect, and its preparation method - Google Patents

A health food with lung nourishing effect, and its preparation method Download PDF

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CN111903973A
CN111903973A CN202010769864.5A CN202010769864A CN111903973A CN 111903973 A CN111903973 A CN 111903973A CN 202010769864 A CN202010769864 A CN 202010769864A CN 111903973 A CN111903973 A CN 111903973A
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powder
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health food
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nourishing effect
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李爱民
赵明明
李永强
李颖
邢岩
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Guozhen Health Technology Beijing Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01Instant products; Powders; Flakes; Granules
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/198Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/20Malt products
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/68Protozoa, e.g. flagella, amoebas, sporozoans, plasmodium or toxoplasma
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06Fungi, e.g. yeasts
    • A61K36/062Ascomycota
    • A61K36/066Clavicipitaceae
    • A61K36/068Cordyceps
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13Coniferophyta (gymnosperms)
    • A61K36/15Pinaceae (Pine family), e.g. pine or cedar
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00Drugs for disorders of the respiratory system
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

A health food with lung nourishing effect and its preparation method, the health food is prepared from the following raw materials by weight: 10-40 parts of cordyceps militaris powder, 2-30 parts of pine pollen, 2-30 parts of naked algae powder and 30-70 parts of fruit and vegetable coarse cereal powder. The health food can activate immune system of human body, realize body balance, improve self-repairing ability, and prevent and improve lung damage caused by dust and haze.

Description

A health food with lung nourishing effect, and its preparation method
Technical Field
The invention provides a lung nourishing health food, and belongs to the technical field of health foods.
Background
In recent years, with rapid progress in economy, the natural environment has been destroyed. One environmental change that people pay more attention to is haze, the main component of which is PM2.5, and as the concentration of PM2.5 increases, physical discomfort, especially the abnormality of the respiratory system, can be caused, and clinical manifestations such as cough, expectoration, chest distress, suffocating and asthma can be induced or aggravated.
PM2.5 refers to fine particulate matter with an aerodynamic equivalent diameter less than or equal to 2.5um in ambient air. PM2.5 mainly comprises organic matters, inorganic matters, water-soluble ions, metal elements and the like, wherein the toxicity of polycyclic aromatic hydrocarbons and derivatives thereof in the organic components is the highest, and the toxicity possibly exceeds ROS (reactive oxygen species) generated by the PM2.5 or free radicals carried by the ROS or the free radicals, so that serious oxidative stress injury, immune reaction, inflammatory reaction, DNA breakage and other injuries are caused. In addition, because the physical and chemical properties of the fine particles are not easy to be cleared by the mucociliary system of the upper respiratory tract, the fine particles are very easy to deposit in the alveoli and the lower respiratory tract, and when the clearing capacity of the organism is exceeded, the fine particles can cause partial damage to the lung and even cause the harm to organs of the whole body through gas exchange.
Frequent outbreaks of haze urge out "haze economy", breed a series of antifog haze products, mostly be external products like protective mask, air purifier etc. to get into internal PM2.5 through respiratory, do not have fine reply means. The classic doctor of the Tang Dynasty points out that Sun Simiao: "eat and can eliminate evil and calm viscera, pleasure and refresh will use resources of qi and blood", "treat at all times, use diet therapy first; for those who are not cured by food therapy, the herb is used later, and those who are good for food and do not live well or live suitable for disease are called good working, the magic method of long-term baits and the technique of extreme health preservation. Therefore, the health-care food which combines the harm characteristics of haze and has the functions of nourishing lung and clearing dust is necessary to be researched.
Disclosure of Invention
In view of the above problems in the prior art, the present invention provides a health food with lung nourishing effect, which can achieve body balance, improve self-healing ability, and prevent and improve lung damage caused by dust and haze by activating the immune system of human body.
In order to achieve the purpose, the invention comprises the following technical scheme:
a health food with a lung nourishing effect is prepared from the following raw materials in parts by weight: 10-40 parts of cordyceps militaris powder, 2-30 parts of pine pollen, 2-30 parts of naked algae powder and 30-70 parts of fruit and vegetable coarse cereal powder.
The health food with the lung nourishing effect is preferably prepared from the following raw materials in parts by weight: 20-26 parts of cordyceps militaris powder, 9-12 parts of pine pollen, 9-12 parts of naked algae powder and 50-60 parts of fruit and vegetable coarse cereal powder.
The health food with lung nourishing effect as described above, preferably, the cordyceps militaris powder is prepared by the following method: taking a fresh cordyceps militaris sporocarp, drying, crushing and sieving to obtain the cordyceps militaris sporocarp powder with the cordycepin content of 1 wt%.
The health food with the lung nourishing effect is prepared by the following steps: the collected pine pollen is sieved by a 120-mesh sieve, and the content of crude polysaccharide is 1.5 wt%. Preferably, the frequency of the wall breaking machine is 55 Hz-60 Hz, and the pressure of the air source is 0.7-1.0 MPa.
Preferably, the gymnocypris meal is an aqueous extract or an alcohol extract of gymnocypris, and the content of beta-glucan is 50 wt%.
Preferably, the fruit, vegetable and coarse cereal powder is prepared from one or more of orange powder, pumpkin powder, carrot powder and malt powder.
Preferably, the fruit and vegetable powder used in the present invention is an aqueous extract of the corresponding raw materials.
In another aspect, the present invention provides a method for preparing a health food having a lung nourishing effect as described above, comprising the steps of: weighing the raw materials, uniformly mixing, and filling to obtain the product.
In another aspect, the invention provides the application of the health food with the lung nourishing effect as a lung nourishing diet therapy product, wherein the oral dose is 5-10 g/day.
For use as described above, preferably, the oral dose is 5 g/day.
The traditional Chinese medicine considers that the cordyceps sinensis enters the lung and kidney meridians, can tonify lung yin and kidney yang, is mainly used for treating kidney deficiency, impotence and spermatorrhea, soreness and pain of waist and knees, weakness after illness, chronic cough and weakness, cough and phlegmatic blood, spontaneous perspiration and night sweat and the like, and is the only traditional Chinese medicine capable of balancing and adjusting yin and yang. Record of Chinese medicine sea: cordyceps militaris, also known as Cordyceps militaris, is sweet and neutral in nature and flavor and enters lung and kidney meridians. The product has effects of invigorating kidney yang, nourishing lung yin, protecting lung, invigorating kidney, replenishing essence, and invigorating qi, and has good therapeutic effect on lung and kidney deficiency, chronic cough, asthma, and cough with excessive phlegm and blood. The Chinese herbal medicine compilation records: the fruiting body and polypide of Cordyceps militaris can also be used as a substitute for Cordyceps. Research shows that the cordyceps militaris can promote epithelial cells of the tracheal wall to maintain normal ion exchange, so that cilia keep rhythmical swing, and sputum accumulation is reduced. In the research of lung allergic inflammation resistance, the cordyceps militaris can reduce inflammatory reaction and trachea wall muscle swelling and thickening to a certain extent.
Pollen Pini is a kind of product which can be used as both medicine and food and is collected in ancient Chinese medical books. Li Shizhen is recorded in Ben Cao gang mu: pine pollen, sweet, warm and nontoxic; moisten heart and lung, tonify qi, dispel wind to stop bleeding. Pollen pini contains more than 200 kinds of nutrients such as saccharides, lipids, proteins, amino acids, nucleic acids, vitamins, minerals, etc., including 8 kinds of amino acids, 14 kinds of vitamins and 24 kinds of trace elements essential for human body, and is known as "micro-nutrient bank" and has multiple functions of regulating immunity, resisting fatigue, improving gastrointestinal function, regulating blood fat and protecting liver.
The Euglena contains more than 20% of protein, essential vitamins, minerals, amino acids, unsaturated fatty acids, etc., is natural and comprehensive nutrient substance, is rich in beta (1, 3) glucan, and can stimulate non-specific immunity of human body.
The invention has the beneficial effects that: the health food of the invention compounds each active component according to specific proportion, wherein: the cordyceps militaris balances yin and yang, nourishes lung and eliminates haze; the compound of the euglena and the pine pollen can condition viscera, nourish immunity and improve healthy energy. The three components are mutually matched, have synergistic effect, and have haze removing effect obviously higher than that of a single component. Experimental results show that the product has obvious effects of promoting phagocytosis, secretion and excretion on PM2.5, and has reliable and safe effects and good taste.
Detailed Description
Sources of some of the raw materials in the following examples:
cordyceps militaris powder: the product is prepared by adopting a fresh cordyceps militaris sporocarp artificially cultured by the Shanghai national treasure enterprise development center through the processes of drying, crushing and sieving, wherein the cordycepin content is 1%.
The pine pollen is prepared by the following method: sieving manually collected pollen pini with a 120-mesh sieve, performing jet milling to break the walls, wherein the frequency of a wall breaking machine is 55 Hz-60 Hz, the pressure of an air source is 0.7-1.0 MPa, and the content of crude polysaccharide is 1.5%.
Naked algae powder: the product of the American Kemin company is prepared by the following method: the euglena seeds are prepared by culturing, concentrating, drying and sieving, and the content of beta-glucan is 50%.
Example 1 health food (I) having lung nourishing effect
1. Formulation of
22 parts of cordyceps militaris powder, 11 parts of pine pollen, 11 parts of pumpkin powder, 11 parts of carrot powder, 11 parts of orange powder, 11 parts of malt powder and 11 parts of naked algae powder.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a sample 1.
Example 2 health food (II) having lung nourishing effect
1. Formulation of
26 parts of cordyceps militaris powder, 9 parts of pine pollen, 11 parts of pumpkin powder, 11 parts of carrot powder, 11 parts of orange powder, 11 parts of malt powder and 9 parts of euglena powder.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a sample 2.
Comparative example 1
1. Formulation of
44 parts of cordyceps militaris powder, 11 parts of pumpkin powder, 11 parts of carrot powder, 11 parts of orange powder and 11 parts of malt powder.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a comparative sample 1.
Comparative example 2
1. Formulation of
44 parts of pine pollen, 11 parts of pumpkin powder, 11 parts of carrot powder, 11 parts of orange powder and 11 parts of malt powder.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a comparative sample 2.
Comparative example 3
1. Formulation of
44 parts of naked algae powder, 11 parts of pumpkin powder, 11 parts of carrot powder, 11 parts of orange powder and 11 parts of malt powder.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a comparative sample 3.
Experimental example 1 evaluation of PM2.5 resistance of sample of example to zebra fish
First, experiment principle
Compared with PM2.5, the nanoparticles can enter the respiratory tract and alveoli of human beings more easily and are deposited in the respiratory tract and alveoli of human beings, and the smaller the particles, the more the particles are deposited, the greater the harm to human bodies is. Therefore, nanoparticles are often used as a replacement material for PM2.5 for toxicological studies, and nano activated carbon is used as the PM2.5 material in the present model.
Macrophages are important immune cells in the animal body and can play a non-specific defense role by phagocytosing foreign materials. Macrophages are able to directly engulf PM2.5 particulate matter. The removal speed of the nano-particles is directly related to the function of macrophages, and the removal of the nano-particles in vivo can be accelerated by enhancing the function of the macrophages.
The nano activated carbon particles entering the zebra fish body can be secreted into the intestinal tract through goblet cells of the intestinal tract and then discharged, and are called as Intestinal Goblet Cell Secretory Pathway (IGCSP). The influence of the test article on the discharge of the nanoparticles is evaluated by utilizing the secretion of the zebra fish nanoparticles into the intestinal tract, and if the test article has the secretion promoting effect, the fact that the nanoparticles in the test article group are secreted into the intestinal tract is found to be more than that in the model group.
After the nano activated carbon particles are secreted to the intestinal tract by the goblet cells, the nano activated carbon needs to be discharged out of the intestinal tract. The zebra fish is fed with dye nile red as intestinal contents, and the excretion promotion strength is quantified by analyzing the total intestinal fluorescence intensity. If the test sample has the function of promoting intestinal peristalsis, the Nile red in the intestinal tract is reduced.
Secondly, evaluating the phagocytic function of macrophage promoted by the sample
1. Concentration group
Model control group
Sample groups prepared for examples and comparative examples:
samples 1 and 2 groups, and comparative samples 1-3 groups 100 mu g/mL;
samples 1 and 2 groups, and comparative samples 1-3 groups 250 mug/mL;
samples 1 and 2 groups, and comparison samples 1-3 groups 500 mug/mL;
2. experimental methods
2.3mg/mL nano activated carbon is used as PM2.5 nano particles and is injected into veins of 2dpf AL zebra fish, 10nL of nano activated carbon is injected into each zebra fish, and a zebra fish PM2.5 phagocytosis model is established at a dose of 23 ng/tail.
Model zebrafish water solution was given to samples at concentrations of 100, 250 and 500 μ g/mL, while a model control group was set with a volume of 3mL per well (experimental group). After the test article is treated, adding neutral red solution to carry out vital body staining on the zebra fish, and after the staining is finished, randomly taking 10 zebra fish from each group to collect pictures under a microscope, and counting the number (N) of macrophages engulfed by the nano activated carbon in the brain; the promotion effect of the sample on the phagocytic function of the macrophages is evaluated according to the number of the macrophages engulfed with the activated carbon, and the calculation formula is as follows:
Figure BDA0002615041250000051
statistical treatment results are expressed as mean + -SE, with p < 0.05 being significantly different using ANOVA and Dunnett's T-test.
3. Results of the experiment
The results are shown in Table 1. The promotion effect of the samples 1 and 2 on the phagocytic function of the macrophages of the zebrafish is less than 0.001. The numbers of macrophages engulfed with nano activated carbon by 100, 250 and 500 mug/mL concentration groups of the sample 1 are 14, 14 and 16 respectively, compared with a model control group (9), the numbers of p are less than 0.001, and the macrophage phagocytosis promoting effects are 56%, 56% and 78% respectively, which shows that the sample has obvious promoting effect on the phagocytosis function of the zebra fish macrophages under the condition of the experimental concentration.
TABLE 1 Zebra fish macrophage phagocytosis data (n ═ 10)
Figure BDA0002615041250000061
P < 0.01, p < 0.001, compared to model control group
Thirdly, evaluating the promoting effect of the sample on the PM2.5 secretion function
1. Concentration group
Model control group
Samples 1 and 2, comparative samples 1-3 groups 25. mu.g/mL
Samples 1 and 2, comparative samples 1-3, 50. mu.g/mL
Samples 1 and 2, comparative samples 1-3 groups 100. mu.g/mL
2. Experimental methods
2.3mg/mL nano activated carbon is used as PM2.5 nano particles and is injected into a yolk sac of 2dpf AL zebra fish (equivalent to intramuscular injection of human beings), and each zebra fish is injected with 30nL, namely a zebra fish PM2.5 secretion model is established at a dose of 69 ng/tail.
Model zebrafish water solutions were given to samples at 25, 50 and 100 μ g/mL concentrations, while a model control group was set with a volume of 3mL per well (experimental group). After the sample is processed, pictures are collected under a microscope, and the number (N) of zebra fishes secreting active carbon in the intestinal tract is observed and calculated; according to the number of zebra fish secreting activated carbon, the promotion effect of the sample on the PM2.5 secretion function is evaluated, and the calculation formula is as follows:
Figure BDA0002615041250000071
statistical treatment results are expressed as mean + -SE, with p < 0.05 being significantly different using ANOVA and Dunnett's T-test.
3. Results of the experiment
The results are shown in Table 2. The results show that: compared with a model control group, the zebra fish PM2.5 secretion function promoting effects of the sample 1 group and the sample 2 group are less than 0.01, and the fact that the samples have the function of promoting the zebra fish PM2.5 secretion under the experimental concentration condition is shown. Wherein, the number of zebra fish secreted by the nano activated carbon in the concentration groups of 25, 50 and 100 mug/mL of the sample 1 is 17, 23 and 26, the secretion rate of the nano activated carbon is 57%, 77% and 87%, respectively, and compared with the model control group (8 tails, the secretion rate of the nano activated carbon is 27%), p is less than 0.05& p is less than 0.001.
TABLE 2 Zebra fish data for PM2.5 promotion (n ═ 10)
Figure BDA0002615041250000072
P < 0.05, p < 0.01, compared to model control
Fourth, the sample has the function of promoting PM2.5 to be excreted through intestinal tract
1. Concentration group
Model control group
Samples 1 and 2, comparative samples 1-3 groups 100. mu.g/mL
Samples 1 and 2, and comparative samples 1-3 250. mu.g/mL
Samples 1, 2 groups, comparative sample 1-3 groups 500. mu.g/mL
2. Experimental methods
Preparing Nile red into 10ng/mL solution by using fish-farming water, putting 5dpf AB zebra fish into the solution for 18h to enable the zebra fish to swallow the Nile red into an intestinal tract, and establishing a zebra fish intestinal motility evaluation model.
Samples were given 100, 250 and 500. mu.g/mL in water while a model control group was set, and 30 zebrafish were treated in each well with a 3mL liquid volume per well. After each group is processed for 6 hours, 10 zebra fishes are randomly taken from each group to be photographed under a fluorescence microscope and data are collected, the intensity of fluorescence signals of intestinal tracts of the zebra fishes is analyzed, the promotion effect of the samples on intestinal excretion is evaluated according to the statistical analysis result of the intensity of the fluorescence signals of the intestinal tracts, and the statistical processing result is expressed by mean +/-SE. The intestinal excretion promoting effect is calculated as follows:
Figure BDA0002615041250000081
statistical analysis using analysis of variance and Dunnett's T-test showed significant differences with p < 0.05.
3. Results of the experiment
The results are shown in Table 3. The results show that: the promotion rates of the groups of the samples 1 and the groups of the samples 2 on the intestinal peristalsis of the zebra fish are both less than 0.001, which shows that the samples 1 and the samples 2 have obvious promotion effects on the intestinal peristalsis of the zebra fish under the condition of the experimental concentration. Wherein, the intestinal fluorescence signal intensities of zebra fish in the concentration groups of 100, 250 and 500 mug/mL of the sample 1 are 263778, 243428 and 151896 pixels respectively, compared with the model control group (490900 pixels), the p is less than 0.001, and the intestinal excretion promotion effect is 46%, 50% and 69% respectively.
TABLE 3 intensity of fluorescent signal of zebra fish intestinal after sample treatment (n 10)
Figure BDA0002615041250000091
P < 0.001 in comparison with model control group
Fifth, conclusion of experiment
The above experimental results show that both sample 1 and sample 2 have significant effects of promoting phagocytosis, secretion and excretion of PM 2.5.

Claims (9)

1. A health food with a lung nourishing effect is characterized by being prepared from the following raw materials in parts by weight: 10-40 parts of cordyceps militaris powder, 2-30 parts of pine pollen, 2-30 parts of naked algae powder and 30-70 parts of fruit and vegetable coarse cereal powder.
2. The health food with the lung nourishing effect according to claim 1, which is prepared from the following raw materials in parts by weight: 20-26 parts of cordyceps militaris powder, 9-12 parts of pine pollen, 9-12 parts of naked algae powder and 50-60 parts of fruit and vegetable coarse cereal powder.
3. The health food having a lung-nourishing effect according to claim 1 or 2, wherein the cordyceps militaris powder is prepared by the following method: taking a fresh cordyceps militaris sporocarp, drying, crushing and sieving to obtain the cordyceps militaris sporocarp powder with the cordycepin content of 1 wt%.
4. The health food with a lung nourishing effect according to claim 1 or 2, wherein the pine pollen is prepared by the following method: the collected pine pollen is sieved by a 120-mesh sieve, and the content of crude polysaccharide is 1.5 wt%.
5. The health food having a lung nourishing effect according to claim 1 or 2, wherein the gymnema powdered is an aqueous or alcoholic extract of gymnema, and the content of β -glucan is 50 wt%.
6. The health food with a lung nourishing effect according to claim 1 or 2, wherein the fruit, vegetable and coarse cereal powder is prepared from one or more of orange powder, pumpkin powder, carrot powder and malt powder.
7. A method for preparing a health food having a lung-nourishing effect according to any one of claims 1 to 6, comprising the steps of: weighing the raw materials, uniformly mixing, and filling to obtain the product.
8. Use of a health food with lung nourishing effect according to any of claims 1-6 as a lung nourishing dietetic product, wherein the oral dosage is 5-10 g/day.
9. The use of claim 8, wherein the oral dose is 5 g/day.
CN202010769864.5A 2020-08-03 2020-08-03 A health food with lung nourishing effect, and its preparation method Pending CN111903973A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101380102A (en) * 2008-09-25 2009-03-11 杭州金日生物科技有限公司 Selenium rich pine pollen health food and preparation method thereof
CN104095182A (en) * 2014-06-16 2014-10-15 李卫平 Food with protection effect on lung injury caused by fog and haze
CN106177014A (en) * 2016-08-26 2016-12-07 成都润馨堂药业有限公司 A kind of Cordyceps militaris (L.) Link. granule of enhancing immunity and preparation method thereof
CN106490529A (en) * 2016-10-19 2017-03-15 烟台新时代健康产业有限公司 A kind of collagen oligopeptide health-care food composition of enhancing immunity and preparation
CN108077706A (en) * 2017-12-29 2018-05-29 江苏康能生物工程股份有限公司 A kind of Cordyceps militaris solid beverage of anti-fog haze protection lung tissue
CN108925992A (en) * 2018-08-08 2018-12-04 林向阳 Protect liver supports lung, health food of runchang detoxification and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101380102A (en) * 2008-09-25 2009-03-11 杭州金日生物科技有限公司 Selenium rich pine pollen health food and preparation method thereof
CN104095182A (en) * 2014-06-16 2014-10-15 李卫平 Food with protection effect on lung injury caused by fog and haze
CN106177014A (en) * 2016-08-26 2016-12-07 成都润馨堂药业有限公司 A kind of Cordyceps militaris (L.) Link. granule of enhancing immunity and preparation method thereof
CN106490529A (en) * 2016-10-19 2017-03-15 烟台新时代健康产业有限公司 A kind of collagen oligopeptide health-care food composition of enhancing immunity and preparation
CN108077706A (en) * 2017-12-29 2018-05-29 江苏康能生物工程股份有限公司 A kind of Cordyceps militaris solid beverage of anti-fog haze protection lung tissue
CN108925992A (en) * 2018-08-08 2018-12-04 林向阳 Protect liver supports lung, health food of runchang detoxification and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杨英利等: "3 种淡水藻对三唑磷的降解研究", 《环境科学研究》 *

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