CN111849760A - PCR reaction device - Google Patents
PCR reaction device Download PDFInfo
- Publication number
- CN111849760A CN111849760A CN202010898416.5A CN202010898416A CN111849760A CN 111849760 A CN111849760 A CN 111849760A CN 202010898416 A CN202010898416 A CN 202010898416A CN 111849760 A CN111849760 A CN 111849760A
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- CN
- China
- Prior art keywords
- pcr reaction
- reaction plate
- upper cover
- plate
- temperature control
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
Abstract
The invention relates to a PCR reaction device, which comprises a temperature control table, a PCR reaction plate and a PCR reaction plate gland bush, wherein the PCR reaction plate is arranged on the temperature control table, the temperature control table is provided with an adsorption component, the PCR reaction plate gland bush is arranged above the PCR reaction plate, the PCR reaction plate gland bush is provided with a clamping groove, a magnet is arranged in the clamping groove, the clamping groove is matched with the PCR reaction plate upper cover, the PCR reaction plate gland bush is provided with a magnetic attraction component, the PCR reaction plate comprises an upper cover and a bottom plate, the upper cover is arranged above the bottom plate and is provided with a magnet, and the polarity of the magnet is opposite to that of the magnet in the clamping groove of the PCR reaction plate. The invention adopts double elastic connection, so that when the sealed PCR reaction plate is subjected to high-low temperature circulation, the PCR reaction plate can be completely pressed under the action of elastic force and magnet attraction even if the PCR reaction plate is subjected to expansion caused by heat and contraction caused by cold when subjected to high and low temperatures, the PCR reaction plate is ensured to be in close contact with the temperature control table, the PCR reaction plate can be ensured to be completely sealed, and the pollution caused by liquid leakage in the medical inspection process is avoided.
Description
Technical Field
The invention relates to the field of biomedical inspection and analysis instruments, in particular to a PCR reaction device.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying specific DNA fragments, which can be viewed as specific DNA replication in vitro. In the digital PCR analysis and detection, a reaction system is divided into tens of thousands of micro-droplets in a PCR reaction plate, the PCR reaction plate is in an open state before division, and the PCR reaction plate needs to be sealed after the generation of the micro-droplets is finished. After being sealed, the PCR reaction plate is arranged on the temperature control platform and is tightly attached to the temperature control module, and the refrigerating capacity or the heating capacity of the temperature control module can penetrate through the PCR plate and be conducted to the reaction liquid. However, since the PCR reaction plate is directly placed on the temperature control table, it is difficult to ensure complete flatness of the temperature control table and the PCR plate, and a large amount of air exists between the temperature control table and the PCR plate to affect the conduction of cooling capacity or heating capacity. In order to realize rapid temperature rise or temperature reduction of the PCR reaction solution, the air residual quantity between the temperature control table and the PCR plate needs to be reduced, the PCR plate is ensured to be tightly attached to the plane of the heating refrigerating sheet, and the refrigerating capacity or the heating capacity of the temperature control table can be rapidly and uniformly conducted to the PCR reaction solution.
Disclosure of Invention
The invention aims to solve the problems in the prior art, and provides a PCR reaction plate sealing device which has an automatic sealing function, can finish automatic PCR reaction plate sealing in a machine, can finish elastic locking in the temperature control process, ensures that the PCR reaction plate is tightly attached to a temperature control table, ensures that consumable materials are always in close contact with the temperature control table, and can quickly and uniformly conduct the refrigerating capacity or heating capacity of a heating refrigerating sheet of the temperature control table to PCR reaction liquid.
The specific technical scheme is as follows: the utility model provides a PCR reaction unit, its characterized in that, includes the control by temperature change platform, PCR reaction plate and PCR reaction plate gland, and the PCR reaction plate setting is on the control by temperature change platform, and the control by temperature change platform is equipped with the absorption subassembly, PCR reaction plate gland sets up in PCR reaction plate top, and the PCR reaction plate gland is equipped with magnetism and inhales the subassembly, the PCR reaction plate includes upper cover and bottom plate, and the upper cover is located the bottom plate top, and the upper cover is equipped with magnet.
Preferably, the adsorption assemblies are multiple and are arranged at the end part of the temperature control table.
Preferably, the adsorption assembly comprises a first electromagnetic chuck and a reset piece, and the reset piece is arranged between the first electromagnetic chuck and the temperature control table.
As preferred scheme, the subassembly is inhaled to magnetism includes first fitting piece, second fitting piece and gland piece that resets, and first fitting piece and second fitting piece set up respectively at PCR reaction plate gland top surface and bottom surface, and the gland resets and sets up between first fitting piece and gland frame.
As the preferred scheme, the gland of the PCR reaction plate is provided with a clamping groove, a magnet is arranged in the clamping groove, and the clamping groove is matched with the upper cover of the PCR reaction plate.
Preferably, a sealant is disposed between the upper cover and the bottom plate of the PCR reaction plate, and the sealant can be disposed on the upper cover or the bottom plate of the PCR reaction plate.
As a preferred scheme, a fixing frame is arranged on the temperature control platform and is matched with the temperature control platform, and a clamping groove is formed in the fixing frame and is matched with the bottom plate of the PCR reaction plate.
As the preferred scheme, the PCR reaction device further comprises a sucker gripper, the sucker gripper is provided with a second electromagnetic sucker, and the second electromagnetic sucker and the magnetic suction assembly are correspondingly arranged.
The invention has the technical effects that: the PCR reaction device adopts dual elastic connection, so that when the sealed PCR reaction plate is subjected to high-temperature and low-temperature circulation, the PCR reaction plate can be completely pressed under the action of elastic force and magnet attraction even if the PCR reaction plate is subjected to expansion with heat and contraction with cold when subjected to high and low temperatures, the PCR reaction plate is ensured to be in close contact with the temperature control table, the PCR reaction plate can be ensured to be completely sealed, and the pollution caused by liquid leakage in the medical inspection process is avoided.
Drawings
FIG. 1 is a schematic view of a PCR reaction apparatus according to an embodiment of the present invention.
Fig. 2 is a schematic view of a suction cup gripper in accordance with an embodiment of the present invention.
FIG. 3 is a schematic view of a PCR reaction plate according to an embodiment of the present invention.
FIG. 4 is a schematic view of a gland of a PCR reaction plate according to an embodiment of the present invention.
FIG. 5 is a schematic view of a temperature control stage according to an embodiment of the present invention.
FIG. 6 is a schematic diagram of the adsorption of the consumable top cap according to the embodiment of the present invention.
FIG. 7 is a schematic view showing the transfer of the pressing lid of the PCR reaction plate by the gripper of the suction cup according to the embodiment of the present invention.
FIG. 8 is a schematic view showing a PCR reaction plate in an embodiment of the present invention in which a press lid is locked on a temperature control stage.
Detailed Description
The essential features and advantages of the invention will be further explained below with reference to examples, but the invention is not limited to the examples listed.
As shown in fig. 1 to 8, a PCR reaction apparatus of the present embodiment includes a temperature control platform 1, a PCR reaction plate 2 and a PCR reaction plate gland 3, wherein the PCR reaction plate 2 is disposed on the temperature control platform 1, and the temperature control platform 1 is disposed with an adsorption component 4. The PCR reaction plate gland 3 is arranged above the PCR reaction plate 2, the PCR reaction plate gland is provided with a clamping groove, a magnet is arranged in the clamping groove, and the clamping groove is matched with the upper cover of the PCR reaction plate. The PCR reaction plate gland 3 is provided with a magnetic component 5. The PCR reaction plate 2 comprises an upper cover 21 and a bottom plate 22, the upper cover 21 is positioned above the bottom plate 22, the upper cover 21 is provided with a magnetic attraction piece 23, and the polarity of the magnet is opposite to that of the magnet in the gland bush slot of the PCR reaction plate. In the above technical scheme, the temperature control table 1 provides cooling capacity or heating capacity for the PCR reaction plate 2, and the PCR reaction plate gland 3 is used for sucking the PCR reaction plate 2 and pressing the PCR reaction plate 2. The adsorption component 4 of the temperature control platform 1 corresponds to the magnetic attraction component 5 of the PCR reaction plate gland 3, so that the adsorption component and the magnetic attraction component can attract each other, the PCR reaction plate gland compresses the PCR reaction plate, and the PCR reaction plate is ensured to be tightly attached to the plane of the heating and refrigerating sheet of the temperature control platform.
Among the above-mentioned technical scheme, the adaptation inslot of gland is arranged in to the upper cover, after the automatic gland is accomplished to the machine, PCR reaction plate gland 3 reset 53 to apply pressure to PCR reaction plate upper cover, ensure that bottom plate and workstation closely laminate, PCR reaction plate upper cover can take place inflation or shrink when doing heating and refrigeration, upper cover 21 can be by jack-up during the heating inflation, oppression apron resets 53 the compression, upper cover 21 can descend during the refrigeration shrink, oppression apron resets 53 releases, but the arbitrary exerting down force to upper cover 21
The bottom plate 22 of the PCR reaction plate is used for containing PCR reaction liquid, and the upper cover 21 of the PCR reaction plate is used for sealing the bottom plate 22 of the PCR reaction plate. The upper cover 21 is embedded with a magnetic part which is an iron block or a magnet, the PCR reaction plate gland 3 can absorb the upper cover 21 through the magnetic part 5, so that the upper cover 21 can be transferred by controlling the sucker gripper 6, the sucker gripper 6 transfers the upper cover 21 to a proper position above the bottom plate 22 of the PCR reaction plate, and the sucker gripper 6 applies downward pressure to the sealing adhesive between the upper cover 21 and the bottom plate 22 of the PCR reaction plate 2 to seal the PCR reaction plate. The upper cover 21 of the PCR reaction plate is provided with a sealant 211, which may be one or more of 3M glue, pressure sensitive glue, silicon rubber, etc. And the PCR reaction bottom plate and the upper cover are completely sealed by the sealant. In this embodiment, the temperature control table 1 is provided with a fixing frame 7, and the PCR reaction plate 2 is provided on the fixing frame 7.
In this embodiment, adsorption component 4 has a plurality ofly, and a plurality of adsorption component 4 sets up at 1 tip of temperature control platform to make the adsorption affinity more balanced, adsorb more firmly. The adsorption component 4 comprises a first electromagnetic chuck 41 and a reset piece 42, the reset piece 42 is arranged between the first electromagnetic chuck 41 and the temperature control platform 1, and the reset piece is of a spring or rubber and the like and has an elastic structure. The sucking disc is the electro-magnet, and through above-mentioned technical scheme, 1 edge in temperature control platform is equipped with 4 electro-magnets, and accessible circuit control opens the electro-magnet, is the gland state when the electro-magnet has magnetism, can take out the PCR reaction plate when nonmagnetic, and 4 electro-magnets are elastic connection with temperature control platform main part, piece 42 and the locking of first electromagnetic chuck 41 through sucking disc set screw 43 reset.
In this embodiment, the magnetic attraction assembly 5 includes a first fitting member 51, a second fitting member 52 and a cover returning member 53, the first fitting member 51 and the second fitting member 52 are respectively disposed on the top surface and the bottom surface of the PCR reaction plate cover 3, and the cover returning member 53 is disposed between the first fitting member 51 and the cover frame 31. The first fitting member 51 is provided corresponding to the suction cup hand 6, and the second fitting member 52 is provided corresponding to the first electromagnetic suction cup 41 of the temperature control table 1. In this embodiment, the number of the magnetic assemblies is four, the first fitting member 51 and the second fitting member 52 are iron blocks, and the suction cup gripper 6 controls the suction and placement of the gland of the PCR reaction plate through the switch between the electromagnet block on the gripper and the gland frame iron. The elastic connection of first fitting piece and second fitting piece is realized through the gland reset piece to gland frame 31, even if take place expend with heat and contract with cold when the PCR reaction plate receives high low temperature also can push down the PCR reaction plate completely under the effect of elastic force and magnet attraction, ensure PCR reaction plate and temperature control platform in close contact with, can effectively avoid because of expend with heat and contract with cold of PCR reaction plate in high low temperature control process, ensure that the homoenergetic effectively suppresses the PCR reaction plate when high temperature or low temperature. The heat insulation pad or the boss is fixed below the PCR reaction plate gland 3, the heat conducted by the PCR reaction plate can be isolated through heat insulation materials or by reducing the contact area, a small hole is formed in the position where the PCR reaction plate gland is matched with the magnet 23 on the PCR reaction plate upper cover 21, and the gland magnet 32 is embedded in the hole and used for adsorbing the upper cover 21 of the PCR reaction plate.
In this embodiment, the PCR reaction apparatus further includes a sucker gripper 6, the sucker gripper 6 is provided with a second electromagnetic sucker 8, and the second electromagnetic sucker 8 and the magnetic component 5 are correspondingly arranged. The sucker gripper 6 is used for gripping the gland of the PCR reaction plate, 4 contact points of the sucker gripper are electromagnets 81, and the circuit can control the gripping or putting down of the gland of the PCR reaction plate. Be equipped with tongs spring 82 and lock through plunger screw 83 between electro-magnet 81 and the tongs frame 61 for elastic contact is between electro-magnet and the tongs frame 61, can effectively avoid rigid contact to the damage of electro-magnet, tongs frame, sucking disc.
The PCR reaction device of the present embodiment is placed on the stage 10 during use, and the use flow is as follows: placing the bottom plate 22 of the PCR reaction plate in the fixing frame 7 of the PCR reaction plate; adsorbing the upper cover 21 of the PCR reaction plate to the gland 3 of the PCR reaction plate; placing a fixing frame 7 of the PCR reaction plate on the temperature control platform 1, and placing a gland 3 of the PCR reaction plate on the bracket; after the PCR reaction liquid is completely divided in the PCR reaction plate 2, the mechanical arm controls the sucker gripper 6 to grab the PCR reaction plate gland 3 onto the PCR reaction plate, and sealing is completed; after the sealing of the PCR reaction plate is finished, high-temperature and low-temperature circulation is started.
The PCR reaction apparatus of this example employs the principle of double elastic ligation.
First heavy elastic connection: the adaptation inslot of gland is arranged in to the upper cover, after the automatic gland is accomplished to the machine, the piece 53 that resets of PCR reaction plate gland 3 exerts pressure to PCR reaction plate upper cover, ensure that bottom plate and workstation closely laminate, the PCR reaction plate upper cover can take place inflation or shrink when doing heating and refrigeration, upper cover 21 can be by jack-up during the inflation, the oppression apron resets 53 compression, upper cover 21 can descend during the shrink, the oppression apron resets 53 releases, but the arbitrary pushing down force of applying to upper cover 21.
The second elastic connection: the pressure of the restoring member 53 is smaller than that of the restoring members 82 and 42 and, when the upper cover 21 exceeds the elastic stroke of the pressing cover during the thermal expansion, the restoring members 82 and 42 are compressed and the electromagnets 41 and 81 are lifted up to apply the pressing force to the upper cover 21.
In this embodiment, sucking disc tongs electro-magnet, the iron plate, the control by temperature change platform electro-magnet all adopt elastic connection to when sealed PCR reaction plate carries out high low temperature circulation, even if take place expend with heat and contract with cold when the PCR reaction plate receives high low temperature and also can push down the PCR reaction plate completely under the effect of elastic force and magnet attraction, ensure PCR reaction plate and control by temperature change platform in close contact with, can guarantee simultaneously that the PCR reaction plate is sealed completely, thereby avoided appearing liquid leakage in medical science inspection process, lead to polluting.
It should be noted that the above-mentioned preferred embodiments are merely illustrative of the technical concepts and features of the present invention, and are intended to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (8)
1. The utility model provides a PCR reaction unit, its characterized in that, includes the control by temperature change platform, PCR reaction plate and PCR reaction plate gland, and the PCR reaction plate setting is on the control by temperature change platform, and the control by temperature change platform is equipped with the absorption subassembly, PCR reaction plate gland sets up in PCR reaction plate top, and the PCR reaction plate gland is equipped with magnetism and inhales the subassembly, the PCR reaction plate includes upper cover and bottom plate, and the upper cover is located the bottom plate top, and the upper cover is equipped with magnet.
2. The PCR reaction device of claim 1, wherein the adsorption assembly is provided in plurality, and the plurality of adsorption assemblies are disposed at an end of the temperature control table.
3. The PCR reaction device of claim 2, wherein the adsorption component comprises a first electromagnetic chuck and a reset member, and the reset member is arranged between the first electromagnetic chuck and the temperature control platform.
4. The PCR reaction device of claim 1, wherein the magnetic assembly comprises a first fitting member, a second fitting member and a cover returning member, the first fitting member and the second fitting member are respectively disposed on the top surface and the bottom surface of the cover of the PCR reaction plate, and the cover returning member is disposed between the first fitting member and the cover frame.
5. The PCR reaction apparatus of claim 1, wherein the pressing cover of the PCR reaction plate is provided with a slot, and the magnet is arranged in the slot, and the slot is adapted to the upper cover of the PCR reaction plate.
6. The apparatus of claim 1, wherein a sealant is disposed between the upper cover and the bottom plate, and the sealant can be disposed on the upper cover or the bottom plate.
7. The PCR reaction apparatus of claim 1, wherein a fixing frame is disposed on the temperature control stage, the fixing frame is adapted to the temperature control stage, and a slot is formed on the fixing frame and adapted to the bottom plate of the PCR reaction plate.
8. The PCR reaction apparatus of any one of claims 1 to 7 further comprising a suction cup gripper, wherein the suction cup gripper is provided with a second electromagnetic suction cup, and the second electromagnetic suction cup is arranged corresponding to the magnetic assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010898416.5A CN111849760A (en) | 2020-08-31 | 2020-08-31 | PCR reaction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010898416.5A CN111849760A (en) | 2020-08-31 | 2020-08-31 | PCR reaction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111849760A true CN111849760A (en) | 2020-10-30 |
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ID=72967647
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010898416.5A Pending CN111849760A (en) | 2020-08-31 | 2020-08-31 | PCR reaction device |
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| Country | Link |
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| CN (1) | CN111849760A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851149A (en) * | 2022-12-21 | 2023-03-28 | 莫纳(武汉)生物科技有限公司 | Sealing plate film and PCR (polymerase chain reaction) hot cap sealing method |
-
2020
- 2020-08-31 CN CN202010898416.5A patent/CN111849760A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851149A (en) * | 2022-12-21 | 2023-03-28 | 莫纳(武汉)生物科技有限公司 | Sealing plate film and PCR (polymerase chain reaction) hot cap sealing method |
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