CN111822068A - Double-tube single-quantitative pipette and pipetting method thereof - Google Patents

Double-tube single-quantitative pipette and pipetting method thereof Download PDF

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Publication number
CN111822068A
CN111822068A CN202010853139.6A CN202010853139A CN111822068A CN 111822068 A CN111822068 A CN 111822068A CN 202010853139 A CN202010853139 A CN 202010853139A CN 111822068 A CN111822068 A CN 111822068A
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China
Prior art keywords
air bag
pipette
liquid
pipes
pressing
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CN202010853139.6A
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CN111822068B (en
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马云天
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Jiangsu Kehua Medical Instrument Technology Co ltd
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Jiangsu Kehua Medical Instrument Technology Co ltd
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

The invention discloses a double-tube single-quantitative pipette and a pipetting method thereof, wherein the pipette comprises an upper air bag, a vent pipe, a lower air bag and a pipette; the paths of the two air bags for absorbing liquid are not interfered with each other, so that one air bag can absorb the liquid, the other air bag can discharge the liquid quantitatively, and the air bag which originally absorbs the liquid is extruded to discharge redundant liquid samples for recycling after the liquid is discharged, thereby avoiding waste.

Description

Double-tube single-quantitative pipette and pipetting method thereof
Technical Field
The invention relates to a double-tube single quantitative pipette and a pipetting method thereof.
Background
At present, a quantitative pipette is a pipetting tool for sucking a certain amount of liquid and then transferring the liquid to another place, and a conventional quantitative pipette structure is shown in fig. 1, and includes an air bag 91, a liquid storage bag 92, and a quantitative pipette 93, wherein the air bag 91 is connected to an upper end of the liquid storage bag 92, an upper end of the quantitative pipette 93 is inserted from a lower end of the liquid storage bag 92 and extends into the air bag 91 and the liquid storage bag 92, and the peripheral outer side of the quantitative pipette 93 is kept to be hermetically connected with the peripheral inner side of the lower end of the liquid storage bag 92, so that the quantitative pipette with the existing structure is formed, by squeezing the bladder 91 and then releasing, such that liquid is drawn from the dosing pipette 93 to fill the entire dosing pipette 93, excess liquid will overflow the upper end of the dosing pipette 93 into the lower reservoir 92, then the air bag 91 is squeezed again to discharge the liquid in the quantitative pipette 93, thus forming the whole sucking and pipetting process; however, the liquid overflowing the liquid storage bag 92 cannot be discharged by the squeezing air bag 91, so that a part of liquid sample is wasted, and great cost consumption is caused for expensive samples, and in addition, some novice operators have excessive force for squeezing the air bag 91, so that the quantitative pipette 93, the liquid storage bag 92 and the air bag 91 are completely filled with the liquid, and thus, the air pressure is insufficient, so that the pipetting fails; the quantitative pipette structure can derive various deformation structures, for example, chinese patent publication No. CN205126256U, in which a liquid storage bag is communicated with and disposed at one side of the upper end of the quantitative pipette, and the overflow pipe designed to be inclined is used to prevent backflow, so the same drawback exists in this patent, and it is necessary to develop a pipette capable of squeezing, discharging, collecting redundant liquid samples, and preventing a novice operator from having difficulty in controlling squeezing pressure to cause excessive suction.
Disclosure of Invention
Aiming at the defects of the prior art, the invention solves the problems that: provides a double-tube single-quantitative pipette and a pipetting method thereof, which can not only quantitatively pipette liquid, but also squeeze, discharge and collect redundant liquid samples and prevent the defect of excessive suction caused by the difficulty in controlling the squeezing pressure by a novice operator.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a double-tube single-quantitative pipette comprises an upper air bag, a vent pipe, a lower air bag and a pipette; the upper end of the upper air bag is closed, and the lower end of the upper air bag is provided with an upper opening; the lower air bag is positioned right below the upper air bag; the upper end of the lower air bag is closed, and the lower end of the lower air bag is provided with a lower opening; two air pipes are arranged; the two vent pipes are respectively arranged outside two sides of the lower air bag; the upper ends of the two breather pipes extend and meet to form an upper end extending and meeting part; the upper end extension intersection is positioned right above the lower air bag; the upper ends of the extending and meeting positions of the upper ends of the two vent pipes are communicated with the upper opening at the lower end of the upper air bag; the pipette is arranged right below the two vent pipes and the lower air bag; the lower ends of the two vent pipes extend and meet to form a lower end extending and meeting part; the lower end extension intersection is positioned right below the lower opening of the lower air bag; the upper ends of the lower end extension intersections of the two vent pipes are communicated with the lower opening of the lower air bag, and the lower ends of the lower end extension intersections of the two vent pipes are communicated with the upper end of the pipette.
Furthermore, the upper end face of the pipette is connected to the outer side face of the lower end extending intersection of the two vent pipes.
Further, the two vent pipes are respectively connected to the two sides of the lower air bag through plastic pieces in a curing manner.
Further, the pipette is a glass tube.
Further, the pipette is a plastic tube.
Furthermore, a ventilating and water-tight filter element is arranged at the lower opening of the lower air bag; the upper end and the lower end of the ventilation water-blocking filter element respectively extend into the extension intersection of the inner part and the lower end of the lower air bag.
A pipetting method of a double-tube single quantitative pipette comprises the following steps: s1, pressing of the lower air bag: firstly, pressing the lower air bag, then contacting the lower end of the pipette with the liquid level to be sucked, loosening the lower air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid to the extending intersection of the lower ends of the two vent pipes and the interior of the lower air bag; s2, pressing of the upper air bag: pressing the upper air bag, extruding and discharging liquid in the whole pipette and liquid at the lower half part of the extending intersection of the lower ends of the two vent pipes by air pressure in the upper air bag, and realizing pipetting; s3, discharging and collecting residual liquid: the lower air bag is pressed again, so that the liquid in the lower air bag and in the upper half part of the extension intersection of the lower ends of the two vent pipes is discharged from the lower end of the pipette and collected.
A pipetting method of a double-tube single quantitative pipette comprises the following steps: s1, pressing of the upper air bag: firstly, pressing the upper air bag, then contacting the lower end of the pipette with the liquid level to be sucked, loosening the upper air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid to the extending intersection of the lower ends of the two air pipes and the interiors of the two air pipes; s2, pressing of the lower air bag: the lower air bag is pressed, and liquid in the whole pipette and liquid at the extending and meeting part of the lower ends of the two vent pipes are extruded and discharged through air pressure in the lower air bag, so that the aim of pipetting is fulfilled; s3, discharging and collecting residual liquid: the upper balloon is again squeezed so that the liquid in both air ducts is drained from the lower end of the pipette and collected.
The invention has the advantages of
The invention sucks and discharges liquid and cooperates and uses through two gasbags of upper gasbag and lower gasbag, seal between upper end of the lower gasbag and the lower gasbag, extend the aeration route of the upper gasbag to the lower part of the lower gasbag through the breather pipe of both sides of lower end, and the pipette is communicated and designed under the lower gasbag and breather pipe, so the route that two gasbags suck liquid does not interfere each other, can do a gasbag to suck liquid, another gasbag carries on the discharge of the liquid quantitatively, extrude the surplus liquid sample and reclaim and avoid wasting through the gasbag that sucks the liquid originally after discharging the liquid, so the invention has realized and both can remove the liquid quantitatively and reclaim the purpose of the surplus liquid sample, in addition because of the independent aeration route of two gasbags, no matter which gasbag takes the phenomenon of the excessive suction, the other air bag can discharge the quantitative liquid, and the liquid sample pipetting operation is not influenced.
Drawings
Fig. 1 is a schematic structural diagram of the prior art.
Fig. 2 is a schematic structural diagram of embodiment 1 of the present invention.
Fig. 3 is a schematic structural diagram of embodiment 2 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1
As shown in fig. 2, a double-tube single quantitative pipette comprises an upper air bag 1, a vent pipe 3, a lower air bag 2 and a pipette 4; the upper end of the upper air bag 1 is closed, and the lower end of the upper air bag 1 is provided with an upper opening 11; the lower air bag 2 is positioned right below the upper air bag 1; the upper end of the lower air bag 2 is closed, and the lower end of the lower air bag 2 is provided with a lower opening 21; two air pipes 3 are arranged; the two vent pipes 3 are respectively arranged outside the two sides of the lower air bag 2; the upper ends of the two air pipes 3 extend and meet to form an upper end extending and meeting part 31; the upper end extension intersection 31 is located directly above the lower air bag 1; the upper ends of the extending and meeting parts 31 of the upper ends of the two vent pipes 3 are communicated with the upper opening 11 at the lower end of the upper air bag 1; the pipette 4 is arranged right below the two vent pipes 3 and the lower air bag 2; the lower ends of the two air pipes 3 extend and meet to form a lower end extending and meeting part 32; the lower end extension intersection 32 is located directly below the lower opening 21 of the lower bag 2; the upper ends of the lower end extension junctions 32 of the two air pipes 3 are communicated with the lower opening 21 of the lower air bag 2, and the lower ends of the lower end extension junctions 32 of the two air pipes 3 are communicated with the upper end of the pipette 4. Further preferably, the upper end surface of the pipette 4 is connected to the outer lower end surface of the lower end extension junction 32 of the two air tubes 3. Further, the two vent pipes 3 are respectively connected to the two sides of the lower air bag 2 through plastic parts in a curing manner. Further, the pipette 4 is a glass tube or a plastic tube. The interior of the pipette is generally a circular cavity structure, and the pipette capacity is determined by its inner diameter and length.
The present invention is directed to the following two pipetting methods for the upper balloon 1 and the lower balloon 2.
The first method comprises the following steps: as shown in fig. 2, a pipetting method of a double-tube single quantitative pipette comprises the following steps: s1, pressing of the lower air bag: firstly, the lower air bag 2 is pressed, then the lower end of the pipette 4 is contacted with the liquid surface to be sucked, the lower air bag 2 is loosened, liquid is sucked from the lower end of the pipette 4 and fills the whole pipette 4, and the liquid is sucked to the lower end extending junction 32 of the two air pipes 3 and the inside of the lower air bag 2; s2, pressing of the upper air bag: pressing the upper air bag 1, extruding and discharging the liquid in the whole pipetting tube 4 and the liquid in the lower half part of the extending intersection 32 of the lower ends of the two vent tubes 3 through the air pressure in the upper air bag 1, and realizing pipetting; s3, discharging and collecting residual liquid: the lower balloon 2 is again squeezed so that the liquid inside the lower balloon 2 and in the upper half of the lower extending intersection 32 of the two vent tubes 3 is drained from the lower end of the pipette 4 and collected.
And the second method comprises the following steps: as shown in fig. 2, a pipetting method of a double-tube single quantitative pipette comprises the following steps: s1, pressing of the upper air bag: firstly, pressing the upper air bag 1, then contacting the lower end of the pipette 4 with the liquid surface to be sucked, loosening the upper air bag 1, sucking liquid from the lower end of the pipette 4 and filling the whole pipette, and sucking the liquid to the lower end extension intersection 32 of the two air pipes 3 and the insides of the two air pipes 3; s2, pressing of the lower air bag: the lower air bag 2 is pressed, and the liquid in the whole pipette 4 and the liquid at the lower end extension intersection 32 of the two air pipes 3 are extruded and discharged through the air pressure in the lower air bag 2, so that the aim of pipetting is fulfilled; s3, discharging and collecting residual liquid: the upper air bag 1 is pressed again so that the liquid in the two air tubes 3 is discharged from the lower end of the pipette 4 and collected.
Example 2
The present embodiment is different from embodiment 1 in that a filter element 8 for ventilation and water non-passage is provided at a lower opening 21 of the lower air bag 2. As shown in fig. 3, the upper and lower ends of the air-permeable and water-impermeable filter element 8 extend into the inner portion of the lower air bag 2 and the lower end extension junction 32, respectively, so that the pipetting method of the present invention can be preferably as follows: firstly, by extruding the lower air bag 2, when the liquid is sucked by the pipette 4, the path of the liquid entering is automatically closed when the liquid reaches the lower side of the air-permeable and water-impermeable filter element 8, the pipette 4 automatically stops sucking the liquid, and then after the upper air bag 1 is extruded, the pipette 4 and the liquid in the lower end extension intersection 32 are all discharged, so that the liquid transfer is realized, the subsequent process of discharging and recovering the residual liquid is not needed, and the use is simpler and more convenient; of course, the upper bag 1 can be pressed preferentially in the second mode of embodiment 1.
The invention sucks and discharges liquid and cooperates and uses through two gasbags of upper gasbag 1 and lower gasbag 2, seal between upper end of the gasbag 2 of the lower part and the lower end of the upper gasbag 1, extend the aeration route of the gasbag 1 of the upper part to the lower part of the gasbag 2 of the lower part through the breather pipe 3 of both sides of lower end, and the pipette 4 is communicated and designed under gasbag 2 of the lower part and breather pipe 3, so the route that two gasbags suck liquid does not interfere each other, can do a gasbag to suck liquid, another gasbag carries on the quantitative liquid discharge, extrude the surplus liquid sample to reclaim and avoid wasting through the gasbag that sucks liquid originally after discharging liquid, so the invention can both remove liquid and achieve the goal of reclaiming the surplus liquid sample quantitatively, in addition because of the independent aeration route of two gasbags, no matter which gasbag takes the phenomenon of transitional suction, the other air bag can discharge the quantitative liquid, and the liquid sample pipetting operation is not influenced.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (7)

1. A double-tube single-quantitative pipette is characterized by comprising an upper air bag, a vent pipe, a lower air bag and a pipette; the upper end of the upper air bag is closed, and the lower end of the upper air bag is provided with an upper opening; the lower air bag is positioned right below the upper air bag; the upper end of the lower air bag is closed, and the lower end of the lower air bag is provided with a lower opening; two air pipes are arranged; the two vent pipes are respectively arranged outside two sides of the lower air bag; the upper ends of the two breather pipes extend and meet to form an upper end extending and meeting part; the upper end extension intersection is positioned right above the lower air bag; the upper ends of the extending and meeting positions of the upper ends of the two vent pipes are communicated with the upper opening at the lower end of the upper air bag; the pipette is arranged right below the two vent pipes and the lower air bag; the lower ends of the two vent pipes extend and meet to form a lower end extending and meeting part; the lower end extension intersection is positioned right below the lower opening of the lower air bag; the upper ends of the lower end extension intersections of the two vent pipes are communicated with the lower opening of the lower air bag, and the lower ends of the lower end extension intersections of the two vent pipes are communicated with the upper end of the pipette.
2. The dual tube single dosing pipette of claim 1 wherein the upper end face of the pipette is attached to the outer side of the lower end of the two vent tubes where the lower ends extend to meet.
3. The dual tube monodose pipette of claim 1, wherein the two vent tubes are respectively connected to the outside of the lower balloon on both sides by a cured plastic part.
4. The dual tube monodose pipette of claim 1, wherein the pipette is a glass or plastic tube.
5. The dual-tube single dosing pipette of claim 1 wherein a vented non-water permeable filter element is provided at the lower opening of the lower air bladder; the upper end and the lower end of the ventilation water-blocking filter element respectively extend into the extension intersection of the inner part and the lower end of the lower air bag.
6. A method of pipetting a double-barreled monodose pipette according to claim 1, characterized by the following steps: s1, pressing of the lower air bag: firstly, pressing the lower air bag, then contacting the lower end of the pipette with the liquid level to be sucked, loosening the lower air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid to the extending intersection of the lower ends of the two vent pipes and the interior of the lower air bag; s2, pressing of the upper air bag: pressing the upper air bag, extruding and discharging liquid in the whole pipette and liquid at the lower half part of the extending intersection of the lower ends of the two vent pipes by air pressure in the upper air bag, and realizing pipetting; s3, discharging and collecting residual liquid: the lower air bag is pressed again, so that the liquid in the lower air bag and in the upper half part of the extension intersection of the lower ends of the two vent pipes is discharged from the lower end of the pipette and collected.
7. A method of pipetting a double-barreled monodose pipette according to claim 1, characterized by the following steps: s1, pressing of the upper air bag: firstly, pressing the upper air bag, then contacting the lower end of the pipette with the liquid level to be sucked, loosening the upper air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid to the extending intersection of the lower ends of the two air pipes and the interiors of the two air pipes; s2, pressing of the lower air bag: the lower air bag is pressed, and liquid in the whole pipette and liquid at the extending and meeting part of the lower ends of the two vent pipes are extruded and discharged through air pressure in the lower air bag, so that the aim of pipetting is fulfilled; s3, discharging and collecting residual liquid: the upper balloon is again squeezed so that the liquid in both air ducts is drained from the lower end of the pipette and collected.
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Publication number Priority date Publication date Assignee Title
CN114146799A (en) * 2021-12-01 2022-03-08 葛贤杰 Medical treatment is cosmetic with plant liquid grinding extraction equipment

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