CN111957363B - Single quantitative pipette and pipetting method thereof - Google Patents
Single quantitative pipette and pipetting method thereof Download PDFInfo
- Publication number
- CN111957363B CN111957363B CN202010853140.9A CN202010853140A CN111957363B CN 111957363 B CN111957363 B CN 111957363B CN 202010853140 A CN202010853140 A CN 202010853140A CN 111957363 B CN111957363 B CN 111957363B
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- air bag
- pipette
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- pipe
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a single quantitative pipette and a pipetting method thereof, comprising an upper air bag, a lower air bag, a first vent pipe, a second vent pipe and a pipette; according to the invention, the paths of liquid suction by the two air bags are not interfered with each other, so that one air bag can suck liquid, the other air bag quantitatively discharges the liquid, and after the liquid is discharged, the air bag which is originally sucked in the liquid is used for extruding and discharging redundant liquid samples so as to avoid waste.
Description
Technical Field
The invention relates to a single quantitative pipette and a pipetting method thereof.
Background
Currently, a quantitative pipette is a pipetting tool for sucking a certain amount of liquid and transferring the liquid to another place, the conventional quantitative pipette structure is shown in fig. 1, and comprises an air bag 91, a liquid storage bag 92 and a quantitative pipette 93, wherein the air bag 91 is installed at the upper end of the liquid storage bag 92 in a communicating way, the upper end of the quantitative pipette 93 is inserted from the lower end of the liquid storage bag 92 and extends into the air bag 91 and the liquid storage bag 92, the outer periphery of the quantitative pipette 93 is kept in sealing connection with the inner periphery of the lower end of the liquid storage bag 92, the quantitative pipette with the conventional structure is formed, the air bag 91 is pressed and then loosened, so that the liquid is sucked from the quantitative pipette 93 to fill the whole quantitative pipette 93, and excessive liquid overflows into the liquid storage bag 92 below from the upper end of the quantitative pipette 93, and then the air bag 91 is pressed again to drain the liquid in the quantitative pipette 93, so that the whole sucking pipetting process is formed; however, the liquid overflowed into the liquid storage bag 92 cannot be discharged by squeezing the air bag 91, so that a part of liquid sample is wasted, and a great cost is consumed for the valuable sample, and in addition, some novice operators are excessively strong in squeezing the air bag 91, so that the whole of the quantitative pipette 93, the liquid storage bag 92 and the air bag 91 is filled with liquid, and the air pressure is insufficient to cause the liquid transfer failure; the quantitative pipette structure can be derived from various deformation structures, such as a Chinese patent with publication number of CN205126256U, wherein a liquid storage bag is communicated with the upper end side of the quantitative pipette, and backflow is prevented through an overflow pipeline which is obliquely designed, so that the quantitative pipette structure has the same defects, and therefore, a pipette which can extrude, discharge and collect redundant liquid samples and prevent excessive suction defects caused by the fact that a novice operator cannot control extrusion force well is required to be developed.
Disclosure of Invention
Aiming at the defects of the prior art, the invention solves the problems that: the single quantitative pipette and the pipetting method thereof can quantitatively pipette and squeeze, discharge and collect redundant liquid samples, and prevent the defect of excessive suction caused by the fact that a novice operator cannot control squeezing force.
In order to solve the problems, the invention adopts the following technical scheme:
a single quantitative pipette, which comprises an upper air bag, a lower air bag, a first vent pipe, a second vent pipe and a pipette; the upper end of the upper air bag is closed, and one side of the lower end of the upper air bag is provided with an upper opening; the lower air bag is positioned below the upper air bag; the upper end of the lower air bag is closed, and one side of the lower end of the lower air bag is provided with a lower opening; the upper end of the first vent pipe is communicated and connected to an upper opening at one side of the lower end of the upper air bag; the upper end of the second vent pipe is communicated and connected to a lower opening at one side of the lower end of the lower air bag; the pipette is arranged below the first ventilation pipe and the second ventilation pipe; the first vent pipe and the second vent pipe are respectively positioned at two sides above the pipette; the lower ends of the first ventilation pipe and the second ventilation pipe are intersected towards the middle to form an intersection; the upper end of the pipette is communicated and connected to the right lower end of the junction.
Further, the upper air bag, the lower air bag, the first ventilation pipe and the second ventilation pipe are connected through plastic parts in a solidifying mode.
Further, the lower ends of the first ventilation pipe and the second ventilation pipe are respectively provided with an inclined pipe body; the inclined pipe body is inclined towards the middle to be intersected and form a conical structure with a large upper part and a small lower part.
Further, the lower ends of the first ventilation pipe and the second ventilation pipe are respectively provided with a horizontal pipe body; the horizontal pipe body horizontally extends towards the middle to form a junction.
Further, the pipette is a glass tube.
Further, the pipette is a plastic tube.
A pipetting method for a single quantitative pipette, comprising the steps of: s1, pressing a lower air bag: firstly, pressing a lower air bag, then, contacting the lower end of the pipette with the liquid level to be sucked, loosening the lower air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into a second vent pipe through a junction; s2, pressing an upper air bag: pressing the upper air bag, extruding and discharging liquid in the whole pipette and part of liquid at the junction through air pressure in the upper air bag, so as to realize pipetting; s3, discharging and collecting residual liquid: the lower bladder is again squeezed so that the liquid in the second vent tube and another portion of the liquid at the junction are expelled from the lower end of the pipette and collected.
A pipetting method for a single quantitative pipette, comprising the steps of: s1, pressing an upper air bag: firstly, pressing an upper air bag, then, contacting the lower end of the pipette with the liquid level to be sucked, loosening the upper air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into a first vent pipe through a junction; s2, pressing a lower air bag: pressing the lower air bag, extruding and discharging the liquid in the whole pipette and part of liquid at the junction through the air pressure in the lower air bag, so as to realize the purpose of pipetting; s3, discharging and collecting residual liquid: the upper bladder is again squeezed so that the liquid in the first vent tube and another portion of the liquid at the junction are expelled from the lower end of the pipette and collected.
The beneficial effects of the invention are that
The invention has the advantages that the liquid is sucked and discharged through the cooperation of the upper air bag and the lower air bag, the lower end of the upper air bag and the upper end of the lower air bag are sealed, the lower ends of the upper air bag and the lower air bag are respectively connected to the upper end of a pipette through the first ventilation pipe and the second ventilation pipe in a crossing way, so that the paths of sucking the liquid by the two air bags are not interfered with each other, one air bag can suck the liquid, the other air bag quantitatively discharges the liquid, and the residual liquid sample is discharged through the air bag which sucks the liquid originally for extrusion and is recycled, so that the waste is avoided.
Drawings
Fig. 1 is a schematic diagram of a prior art structure.
Fig. 2 is a schematic structural diagram of embodiment 1 of the present invention.
Fig. 3 is a schematic structural diagram of embodiment 2 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1
As shown in fig. 2, a single-dose pipette comprises an upper balloon 1, a lower balloon 2, a first vent tube 5, a second vent tube 6, and a pipette 4; the upper end of the upper air bag 1 is closed, and an upper opening 11 is arranged at one side of the lower end of the upper air bag 1; the lower air bag 2 is positioned below the upper air bag 1; the upper end of the lower air bag 2 is closed, and one side of the lower end of the lower air bag 2 is provided with a lower opening 21; the upper end of the first vent pipe 5 is communicated and connected to an upper opening 11 at one side of the lower end of the upper air bag 1; the upper end of the second vent pipe 6 is communicated and connected to a lower opening 21 at one side of the lower end of the lower air bag 2; the pipette 4 is arranged below the first ventilation pipe 5 and the second ventilation pipe 6; the first ventilation pipe 5 and the second ventilation pipe 6 are respectively positioned at two sides above the pipette 4; the lower ends of the first ventilation pipe 5 and the second ventilation pipe 6 are intersected towards the middle to form an intersection 56; the upper end of the pipette 4 is connected in communication with the lower end of the junction 56. Further preferably, the upper airbag 1, the lower airbag 2, the first vent pipe 5 and the second vent pipe 6 are connected by curing the plastic member 3. Further, the lower ends of the first ventilation pipe 5 and the second ventilation pipe 6 are respectively provided with an inclined pipe body 7; the inclined pipe body 7 is inclined towards the middle to meet and form a conical structure with a large upper part and a small lower part. Further, the pipette 4 is a glass tube or a plastic tube. The interior of the pipette is generally a circular cavity structure, with the pipette volume being determined by its inside diameter and length.
Example 2
In this embodiment, referring to embodiment 1, the difference is that the first ventilation pipe 5 and the second ventilation pipe 6 meet in a manner as shown in fig. 3, and a horizontal pipe body 8 is respectively disposed at the lower ends of the first ventilation pipe 5 and the second ventilation pipe 6; the horizontal tubes 8 extend horizontally toward the middle to meet to form a junction 56.
The present invention is directed to the following two pipetting methods for the upper balloon 1 and the lower balloon 2.
First kind: as shown in fig. 2 and 3, the steps are as follows: s1, pressing a lower air bag: firstly, the lower air bag 2 is pressed, then the lower end of the pipette 4 is contacted with the liquid level to be sucked, the lower air bag 2 is loosened, the liquid is sucked from the lower end of the pipette 4 and fills the whole pipette 4, and the liquid is sucked into the second vent pipe 6 through the junction 56; s2, pressing an upper air bag: pressing the upper air bag 1, extruding and discharging the liquid in the whole pipette 4 and part of liquid at the junction 56 by the air pressure in the upper air bag 1 to realize pipetting; s3, discharging and collecting residual liquid: the lower bladder 2 is again squeezed so that the liquid in the second vent tube 6 and another part of the liquid at junction 56 is expelled from the lower end of the pipette 4 and collected.
Second kind: as shown in fig. 2 and 3, the steps are as follows: s1, pressing an upper air bag: the upper bladder 1 is first pressed and then the lower end of the pipette 4 is brought into contact with the liquid surface to be sucked, the upper bladder 1 is released, the liquid is sucked from the lower end of the pipette 4 and fills the whole pipette 4, and the liquid is sucked into the first vent pipe 5 through the junction 56; s2, pressing a lower air bag: pressing the lower air bag 2, extruding and discharging the liquid in the whole pipette 4 and part of liquid at the junction 56 by the air pressure in the lower air bag 2, so as to realize the purpose of pipetting; s3, discharging and collecting residual liquid: the upper bladder 1 is again squeezed so that the liquid in the first vent tube 5 and another part of the liquid at the junction 56 is expelled from the lower end of the pipette 4 and collected.
The invention sucks and discharges the liquid through the cooperation of the two air bags of the upper air bag 1 and the lower air bag 2, the lower end of the upper air bag 1 and the upper end of the lower air bag 2 are sealed, the lower ends of the upper air bag 1 and the lower air bag 2 are respectively connected with the upper end of the pipette 4 through the first ventilation pipe 5 and the second ventilation pipe 6 in a crossing way, thus the paths of sucking the liquid by the two air bags are not interfered with each other, one air bag can suck the liquid, the other air bag quantitatively discharges the liquid, and after the liquid is discharged, the excess liquid sample is extruded and discharged through the air bag which sucks the liquid originally to recycle, thereby avoiding waste.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (8)
1. A single quantitative pipette is characterized by comprising an upper air bag, a lower air bag, a first vent pipe, a second vent pipe and a pipette; the upper end of the upper air bag is closed, and one side of the lower end of the upper air bag is provided with an upper opening; the lower air bag is positioned below the upper air bag; the upper end of the lower air bag is closed, and one side of the lower end of the lower air bag is provided with a lower opening; the upper end of the first vent pipe is communicated and connected to an upper opening at one side of the lower end of the upper air bag; the upper end of the second vent pipe is communicated and connected to a lower opening at one side of the lower end of the lower air bag; the pipette is arranged below the first ventilation pipe and the second ventilation pipe; the first vent pipe and the second vent pipe are respectively positioned at two sides above the pipette; the lower ends of the first ventilation pipe and the second ventilation pipe are intersected towards the middle to form an intersection; the upper end of the pipette is communicated and connected to the right lower end of the junction.
2. The single-dose pipette of claim 1, wherein the upper bladder, lower bladder, first vent tube, second vent tube are solidly connected by a plastic piece.
3. The single-dose pipette of claim 1, wherein the lower ends of the first vent pipe and the second vent pipe are respectively provided with an inclined pipe body; the inclined pipe body is inclined towards the middle to be intersected and form a conical structure with a large upper part and a small lower part.
4. The single-dose pipette of claim 1, wherein the lower ends of the first ventilation pipe and the second ventilation pipe are respectively provided with a horizontal pipe body; the horizontal pipe body horizontally extends towards the middle to form a junction.
5. The single-dose pipette of claim 1, wherein the pipette is a glass tube.
6. The single-dose pipette of claim 1, wherein the pipette is a plastic tube.
7. A pipetting method of a single quantitative pipette according to claim 1, characterized by the steps of: s1, pressing a lower air bag: firstly, pressing a lower air bag, then, contacting the lower end of the pipette with the liquid level to be sucked, loosening the lower air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into a second vent pipe through a junction; s2, pressing an upper air bag: pressing the upper air bag, extruding and discharging liquid in the whole pipette and part of liquid at the junction through air pressure in the upper air bag, so as to realize pipetting; s3, discharging and collecting residual liquid: the lower bladder is again squeezed so that the liquid in the second vent tube and another portion of the liquid at the junction are expelled from the lower end of the pipette and collected.
8. A pipetting method of a single quantitative pipette according to claim 1, characterized by the steps of: s1, pressing an upper air bag: firstly, pressing an upper air bag, then, contacting the lower end of the pipette with the liquid level to be sucked, loosening the upper air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into a first vent pipe through a junction; s2, pressing a lower air bag: pressing the lower air bag, extruding and discharging the liquid in the whole pipette and part of liquid at the junction through the air pressure in the lower air bag, so as to realize the purpose of pipetting; s3, discharging and collecting residual liquid: the upper bladder is again squeezed so that the liquid in the first vent tube and another portion of the liquid at the junction are expelled from the lower end of the pipette and collected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010853140.9A CN111957363B (en) | 2020-08-22 | 2020-08-22 | Single quantitative pipette and pipetting method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010853140.9A CN111957363B (en) | 2020-08-22 | 2020-08-22 | Single quantitative pipette and pipetting method thereof |
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| Publication Number | Publication Date |
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| CN111957363A CN111957363A (en) | 2020-11-20 |
| CN111957363B true CN111957363B (en) | 2023-07-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010853140.9A Active CN111957363B (en) | 2020-08-22 | 2020-08-22 | Single quantitative pipette and pipetting method thereof |
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| Country | Link |
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| CN (1) | CN111957363B (en) |
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| WO2014173373A2 (en) * | 2013-04-23 | 2014-10-30 | Ma Ronghua | Automatic-suction selectable-quantity micro-volume blood sampling tube |
| CN208627334U (en) * | 2018-04-10 | 2019-03-22 | 吴政钢 | A kind of high school chemical experiment liquid-transfering device |
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| CN111957363A (en) | 2020-11-20 |
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