CN111841676B - Multi-range quantitative blood sampling pipette and blood sampling pipetting method thereof - Google Patents
Multi-range quantitative blood sampling pipette and blood sampling pipetting method thereof Download PDFInfo
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- CN111841676B CN111841676B CN202010853147.0A CN202010853147A CN111841676B CN 111841676 B CN111841676 B CN 111841676B CN 202010853147 A CN202010853147 A CN 202010853147A CN 111841676 B CN111841676 B CN 111841676B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention discloses a multi-range quantitative blood sampling pipette and a blood sampling and pipetting method thereof, wherein the multi-range quantitative blood sampling pipette comprises a top air bag, a vent pipe, at least one lower air bag, a pipette, at least one horizontal connecting pipe and a trace blood sampling pipe; the invention realizes the function of collecting blood by installing the trace blood collecting tube below the pipette and collecting blood by the capillary principle of the trace blood collecting tube, and designs the ventilation position of the upper end of the trace blood collecting tube between the outer side of the periphery of the trace blood collecting tube and the inner wall of the cavity of the pipette or designs the ventilation position of the upper end of the trace blood collecting tube on the side wall of one side of the pipette, so that a through hole is not required to be formed on the trace blood collecting tube, and the defects of poor yield and high process difficulty of forming the through hole on the trace blood collecting tube are avoided.
Description
Technical Field
The invention relates to a multi-range quantitative blood sampling pipette and a blood sampling pipetting method thereof.
Background
Currently, a quantitative pipette is a pipetting tool for sucking a certain amount of liquid and transferring the liquid to another place, the conventional quantitative pipette structure is shown in fig. 1, and comprises an air bag 91, a liquid storage bag 92 and a quantitative pipette 93, wherein the air bag 91 is installed at the upper end of the liquid storage bag 92 in a communicating way, the upper end of the quantitative pipette 93 is inserted from the lower end of the liquid storage bag 92 and extends into the air bag 91 and the liquid storage bag 92, the outer periphery of the quantitative pipette 93 is kept in sealing connection with the inner periphery of the lower end of the liquid storage bag 92, the quantitative pipette with the conventional structure is formed, the air bag 91 is pressed and then loosened, so that the liquid is sucked from the quantitative pipette 93 to fill the whole quantitative pipette 93, and excessive liquid overflows into the liquid storage bag 92 below from the upper end of the quantitative pipette 93, and then the air bag 91 is pressed again to drain the liquid in the quantitative pipette 93, so that the whole sucking pipetting process is formed; however, the liquid overflowed into the liquid storage bag 92 cannot be discharged by squeezing the air bag 91, so that a part of liquid sample is wasted, and a great cost is consumed for the valuable sample, and in addition, some novice operators are excessively strong in squeezing the air bag 91, so that the whole of the quantitative pipette 93, the liquid storage bag 92 and the air bag 91 is filled with liquid, and the air pressure is insufficient to cause the liquid transfer failure; the quantitative pipette structure can be derived into various deformation structures, such as a Chinese patent with publication number of CN205126256U, wherein a liquid storage bag is communicated with the upper end side of the quantitative pipette, and backflow is prevented through an overflow pipeline which is obliquely designed, so that the quantitative pipette structure has the same defects, and therefore, a pipette which can extrude, discharge and collect redundant liquid samples and prevent excessive suction defects caused by the fact that a novice operator cannot control extrusion force is required to be developed; in addition, the existing quantitative pipette only has one measuring range, so that the function is single, and the quantitative pipette with multiple measuring ranges needs to be developed; finally, it is also an important development of the present invention to be able to introduce a structure integrating blood collection and pipetting after achieving the above-mentioned problems.
Disclosure of Invention
Aiming at the defects of the prior art, the invention solves the problems that: the multi-range quantitative blood sampling pipette and the blood sampling and pipetting method thereof are integrated in blood collection and pipetting, can squeeze, discharge and collect redundant liquid samples, and prevent excessive suction defects caused by the fact that a novice operator cannot control squeezing force well.
In order to solve the problems, the invention adopts the following technical scheme:
a multi-range quantitative blood sampling pipette comprises a top air bag, a vent pipe, at least one lower air bag, a pipette, at least one horizontal connecting pipe and a trace blood sampling pipe; the upper end of the top air bag is closed, and one side of the lower end of the top air bag is provided with a top opening; the lower part of the top air bag is sequentially connected with at least one lower air bag from top to bottom; the upper end of the lower air bag is closed, and one side of the bottom of the lower air bag is provided with a lower opening; a horizontal connecting pipe is horizontally arranged at the lower opening at one side of the bottom of the lower air bag; the pipettes are arranged on the same side of the top air bag and the lower air bag and are distributed in the vertical direction; the upper end of the vent pipe is connected to the top opening at one side of the lower end of the top air bag, and the lower end of the vent pipe is connected to the upper end of the pipette; one end of the horizontal connecting pipe is connected to the lower opening at one side of the bottom of the lower air bag, and the other end of the horizontal connecting pipe is communicated and connected to the side part of the pipette; the horizontal connecting pipe and the pipette are vertically distributed; the trace blood collection tube is arranged below the pipette; the upper end of the micro blood collection tube is inserted and connected into the lower end of the pipette, and the lower end of the micro blood collection tube extends to the lower part of the pipette; the upper end of the micro blood collection tube is communicated with the external air pressure of the pipette.
Further, a cavity with a square cross section is formed in the pipette; the cross section of the outer side of the periphery of the trace blood collection tube is in a circular structure; the outer side of the periphery of the upper end of the micro blood collection tube is connected with the inner wall of the periphery of the cavity of the pipette; a plurality of ventilation gaps are uniformly arranged between the outer sides of the periphery of the micro blood collection tube and the inner wall of the cavity of the pipette.
Further, the periphery of the upper end of the micro blood collection tube is in closed connection with the inside of the lower end of the pipette; a vent is formed in one side below the pipette; the air vent is connected with an air nozzle pipe in a downward extending mode; the lower end face of the air tap tube is positioned above the lower end face of the micro blood sampling tube; the air vent is positioned above the upper end face of the micro blood collection tube.
Further, the pipette is a glass tube or a plastic tube.
Further, two lower air bags are sequentially connected below the top air bag from top to bottom; the two lower airbags include a first lower airbag and a second lower airbag; the first lower air bag is positioned above the second lower air bag; the first lower air bag and the second lower air bag are respectively communicated and connected to the side part of the pipette through horizontal connecting pipes.
A blood sampling and pipetting method of a multi-range quantitative blood sampling pipette comprises the following steps:
s1, blood sampling: the lower end of a trace blood collection tube below the pipette is contacted with blood to be collected, the blood is sucked by capillary action of the trace blood collection tube until the whole trace blood collection tube is filled, and then the blood in the trace blood collection tube is extruded and discharged into diluent by pressing a top air bag or a lower air bag;
s2, pressing a top air bag: firstly, pressing a top air bag, then, contacting the lower end of the pipette with diluent to be sucked, loosening the top air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into a vent pipe and even reaching the inside of the top air bag;
s3, quantitatively pressing the air bag: pressing the first lower air bag and/or the second lower air bag to realize the purpose of quantitative pipetting;
s4, discharging and collecting residual liquid: the top bladder is again squeezed so that the remaining liquid of the vent tube and pipette is expelled and collected.
Further, in the step S3, the first lower air bag is pressed, so that the liquid in the pipette below the horizontal connecting pipe connected to the first lower air bag is completely discharged, and the quantitative pipetting of the maximum range is realized.
Further, in the step S3, the second lower air bag is pressed, so that the liquid in the pipette below the horizontal connecting pipe connected to the second lower air bag is completely discharged, and quantitative pipetting is realized.
In step S3, the second lower air bag is pressed first, so that the liquid in the pipette below the horizontal connecting pipe connected with the second lower air bag is completely discharged, and quantitative pipetting with one measuring range is realized; and then the first lower air bag is pressed, so that liquid in the pipette between the horizontal connecting pipes connected with the first lower air bag and the second lower air bag is completely discharged, and quantitative pipetting of the other measuring range is realized.
The beneficial effects of the invention are that
1. The invention realizes the function of collecting blood by installing the trace blood collecting tube below the pipette and collecting blood by the capillary principle of the trace blood collecting tube, and designs the ventilation position of the upper end of the trace blood collecting tube between the outer side of the periphery of the trace blood collecting tube and the inner wall of the cavity of the pipette or designs the ventilation position of the upper end of the trace blood collecting tube on the side wall of one side of the pipette, so that a through hole is not required to be formed on the trace blood collecting tube, and the defects of poor yield and high process difficulty of forming the through hole on the trace blood collecting tube are avoided.
2. The lower end of the top air bag is sealed with the upper end of the lower air bag, the top air bag is directly ventilated with the upper end of the pipette through the vent pipe, the lower air bag is ventilated with the side part of the pipette through the horizontal connecting pipe, so that the top air bag and the lower air bag are provided with independent and mutually noninterfere paths for ventilation with the pipette, liquid is sucked through the top air bag, liquid is discharged through each lower air bag to achieve the purpose of quantitative pipetting, and redundant liquid samples are discharged through extrusion of the top air bag to achieve recovery and avoid waste; because the top air bag and the lower air bag are provided with independent paths which do not interfere with each other and ventilate with the pipette, even if the top air bag is excessively extruded to fill the whole liquid, the quantitative liquid discharge of each lower air bag is not influenced; when the lower part of the top air bag is sequentially connected with the plurality of lower air bags from top to bottom, the invention has the advantages of multiple range selection and more flexible and changeable use.
Drawings
Fig. 1 is a schematic diagram of a prior art structure.
Fig. 2 is a schematic view of the structure of the present invention with a lower bladder.
Fig. 3 is a schematic view of the structure of the present invention with two lower airbags.
Fig. 4 is a schematic view of the structure of the present invention with three lower airbags.
FIG. 5 is a schematic diagram showing the front view of the micropipette and pipette of embodiment 1 of the present invention.
FIG. 6 is a schematic cross-sectional view of a micropipette and pipette according to embodiment 1 of the present invention.
FIG. 7 is a schematic diagram showing the front view of the micropipette and pipette of embodiment 2 of the present invention.
Description of the embodiments
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1
As shown in fig. 2, 5 and 6, a multi-range quantitative blood collection pipette comprises a top air bag 1, a vent pipe 3, at least one lower air bag 2, a pipette 4, at least one horizontal connecting pipe 5 and a micro blood collection pipe 9; the upper end of the top air bag 1 is closed, and one side of the lower end of the top air bag 1 is provided with a top opening 11; the lower part of the top airbag 1 is sequentially connected with at least one lower airbag 2 from top to bottom; the upper end of the lower air bag 2 is closed, and one side of the bottom of the lower air bag 2 is provided with a lower opening 21; a horizontal connecting pipe 5 is horizontally arranged at a lower opening 21 at one side of the bottom of the lower air bag 2; the pipettes 4 are arranged on the same side of the top air bag 1 and the lower air bag 2 and are distributed in the vertical direction; the upper end of the vent pipe 3 is connected to a top opening 11 at one side of the lower end of the top air bag 1, and the lower end of the vent pipe 3 is connected to the upper end of the pipette 4; one end of the horizontal connecting pipe 5 is connected to a lower opening 21 at one side of the bottom of the lower air bag 2, and the other end of the horizontal connecting pipe 5 is connected to the side part of the pipette 4 in a communicating manner; the horizontal connecting pipe 5 and the pipette 4 are vertically distributed. Further, the pipette 4 is a glass or plastic tube, and the interior of the pipette 4 is a generally circular cavity structure, and the pipette volume is determined by its inner diameter and length. At least one scale mark is arranged on the pipette 4; the scale marks are horizontally aligned with the upper end surface of the inner cavity of the horizontal connecting pipe 5. The top air bag 1, the lower air bag 2, the vent pipe 3 and one side above the pipette 4 are all connected through a plastic piece 6 in a solidifying mode. The present embodiment chooses to connect a lower balloon 2 below the top balloon 1. The upper end of the micro blood collection tube 9 is inserted and connected into the lower end of the pipette 4, and the lower end of the micro blood collection tube 9 extends to the lower part of the pipette 4; the upper end of the micro blood collection tube 9 is communicated with the external air pressure of the pipette 4. Further preferably, a cavity with a square cross section is arranged inside the pipette 4; the cross section of the outer sides of the periphery of the trace blood collection tube 9 is in a circular structure; the outer side of the periphery of the upper end of the micro blood collection tube 9 is connected with the inner wall of the periphery of the cavity of the pipette 4; a plurality of ventilation gaps 41 are uniformly formed between the outer sides of the periphery of the micro blood collection tube 9 and the inner wall of the cavity of the pipette 4, so that the ventilation is performed between the outer sides of the periphery of the micro blood collection tube 9 and the inner wall of the cavity of the pipette 4 and the outside through the ventilation gaps 41, and the micro blood collection tube 9 can collect blood by utilizing the capillary principle.
In the pipetting process, two modes can be adopted, namely, the first mode is that the top air bag 1 is directly extruded to perform pipetting, then quantitative pipetting is performed through the extrusion lower air bag 2, and finally, the top air bag 1 is extruded again to discharge residual liquid for recycling; and secondly, directly squeezing the lower air bag 2 to absorb liquid, quantitatively pipetting by squeezing the top air bag 1, and finally squeezing the lower air bag 2 again to discharge residual liquid and recycling.
Example 2
As shown in fig. 3 and 7, in this embodiment, referring to embodiment 1, the difference is the ventilation structure of the micro blood collection tube 9, the number of the lower air bags 2, and the pipetting method, wherein two lower air bags 2 are sequentially connected below the top air bag 1 from top to bottom; the two lower airbags 2 include a first lower airbag 2a and a second lower airbag 2b; the first lower air bag 2a is positioned above the second lower air bag 2b; the first lower air bag 2a and the second lower air bag 2b are respectively connected to the side part of the pipette 4 through a horizontal connecting pipe 5 in a communicating way; the periphery of the upper end of the micro blood collection tube 9 is in closed connection with the inside of the lower end of the pipette 4; a vent 43 is formed in one side below the pipette 4; the air vent 43 is connected with an air tap pipe 42 in a downward extending way; the lower end face of the air tap tube 42 is positioned above the lower end face of the micro blood sampling tube 9; the air vent 43 is positioned above the upper end face of the micro blood collection tube 9. In this embodiment, the upper end of the micro blood collection tube 9 is vented to the outside through the vent 43 on the side of the pipette 4.
A blood sampling and pipetting method of a multi-range quantitative blood sampling pipette comprises the following steps:
s1, blood sampling: the lower end of the micro blood collection tube 9 below the pipette 4 is contacted with blood to be collected, the blood is sucked by capillary action of the micro blood collection tube 9 until the whole micro blood collection tube 9 is filled, and then the blood in the micro blood collection tube 9 is extruded and discharged into diluent by pressing the top air bag 1 or the lower air bag 2.
S2, pressing a top air bag: the top bladder 1 is first pressed and then the lower end of the pipette 4 is brought into contact with the diluent to be sucked, the top bladder 1 is released, the liquid is sucked from the lower end of the pipette 4 and fills the whole pipette 4, and the liquid may be sucked into the vent tube 3 even into the top bladder 1.
S3, quantitatively pressing the air bag: pressing the first lower air bag 2a and/or the second lower air bag 2b to realize quantitative pipetting; the quantitative pipetting is performed in three modes, and can be selected according to actual requirements. The first lower air bag 2a is pressed, so that liquid in the pipette 4 below the horizontal connecting pipe 5 connected with the first lower air bag 2a is completely discharged, and quantitative pipetting with the maximum range is realized; the second lower air bag 2b is pressed, so that liquid in the pipette 4 below the horizontal connecting pipe 5 connected with the second lower air bag 2b is completely discharged, and quantitative pipetting is realized; thirdly, the second lower air bag 2b is pressed firstly, so that liquid in the pipette below the horizontal connecting pipe 5 connected with the second lower air bag 2b is completely discharged, and quantitative pipetting with one measuring range is realized; then, the first lower air bag 2a is pressed, so that the liquid in the pipette 4 between the horizontal connecting pipes 5 connected with the first lower air bag 2a and the second lower air bag 2b is completely discharged, and the quantitative pipetting of the other measuring range is realized.
S4, discharging and collecting residual liquid: the top bladder 1 is pressed again so that the remaining liquid of the vent tube 3 and pipette 4 is expelled and collected.
The present invention exemplifies two embodiments, but is not limited thereto, and it is possible to connect three lower airbags 2, four lower airbags 2, five lower airbags 2, etc. in order from top to bottom below the top airbag 1 with reference to embodiment 2, as shown in fig. 4, and thus have more ranges.
The lower end of the top air bag 1 and the upper end of the lower air bag 2 are sealed, the top air bag 1 is directly ventilated with the upper end of the pipette 4 through the vent pipe 3, the lower air bag 2 is ventilated with the side part of the pipette 4 through the horizontal connecting pipe 5, so that the top air bag 1 and the lower air bag 2 are respectively provided with independent and noninterference paths for ventilation with the pipette, liquid is sucked through the top air bag 1, the liquid is discharged through each lower air bag 2 to realize quantitative pipetting, and redundant liquid samples are discharged through the extrusion of the top air bag 1 to realize recovery and avoid waste; because the top air bag 1 and the lower air bag 2 are provided with independent paths which do not interfere with each other and are ventilated with the pipette, the quantitative liquid discharge of each lower air bag 2 is not affected even if the top air bag 1 is excessively extruded and filled with the whole liquid; when the lower part of the top airbag 1 is sequentially connected with the plurality of lower airbags 2 from top to bottom, the invention has the advantages of multiple range selection and more flexible and changeable use.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (9)
1. The multi-range quantitative blood sampling pipette is characterized by comprising a top air bag, a vent pipe, at least one lower air bag, a pipette, at least one horizontal connecting pipe and a trace blood sampling pipe; the upper end of the top air bag is closed, and one side of the lower end of the top air bag is provided with a top opening; the lower part of the top air bag is sequentially connected with at least one lower air bag from top to bottom; the upper end of the lower air bag is closed, and one side of the bottom of the lower air bag is provided with a lower opening; a horizontal connecting pipe is horizontally arranged at the lower opening at one side of the bottom of the lower air bag; the pipettes are arranged on the same side of the top air bag and the lower air bag and are distributed in the vertical direction; the upper end of the vent pipe is connected to the top opening at one side of the lower end of the top air bag, and the lower end of the vent pipe is connected to the upper end of the pipette; one end of the horizontal connecting pipe is connected to the lower opening at one side of the bottom of the lower air bag, and the other end of the horizontal connecting pipe is communicated and connected to the side part of the pipette; the horizontal connecting pipe and the pipette are vertically distributed; the trace blood collection tube is arranged below the pipette; the upper end of the micro blood collection tube is inserted and connected into the lower end of the pipette, and the lower end of the micro blood collection tube extends to the lower part of the pipette; the upper end of the micro blood collection tube is communicated with the external air pressure of the pipette.
2. The multirange quantitative blood collection pipette of claim 1 wherein the interior of the pipette is provided with a cavity having a square cross section; the cross section of the outer side of the periphery of the trace blood collection tube is in a circular structure; the outer side of the periphery of the upper end of the micro blood collection tube is connected with the inner wall of the periphery of the cavity of the pipette; a plurality of ventilation gaps are uniformly arranged between the outer sides of the periphery of the micro blood collection tube and the inner wall of the cavity of the pipette.
3. The multirange quantitative blood collection pipette of claim 1 wherein the circumference of the upper end of the micropositioner is in a closed connection with the interior of the lower end of the pipette; a vent is formed in one side below the pipette; the air vent is connected with an air nozzle pipe in a downward extending mode; the lower end face of the air tap tube is positioned above the lower end face of the micro blood sampling tube; the air vent is positioned above the upper end face of the micro blood collection tube.
4. The multiple-range quantitative blood collection pipette of claim 1 wherein the pipette is a glass tube or a plastic tube.
5. The multiple-range quantitative blood collection pipette of claim 1, wherein two lower air bags are sequentially connected below the top air bag from top to bottom; the two lower airbags include a first lower airbag and a second lower airbag; the first lower air bag is positioned above the second lower air bag; the first lower air bag and the second lower air bag are respectively communicated and connected to the side part of the pipette through horizontal connecting pipes.
6. A method of pipetting blood collection using a multirange quantitative blood collection pipette as recited in claim 5 wherein the steps of:
s1, blood sampling: the lower end of a trace blood collection tube below the pipette is contacted with blood to be collected, the blood is sucked by capillary action of the trace blood collection tube until the whole trace blood collection tube is filled, and then the blood in the trace blood collection tube is extruded and discharged into diluent by pressing a top air bag or a lower air bag;
s2, pressing a top air bag: firstly, pressing a top air bag, then, contacting the lower end of the pipette with diluent to be sucked, loosening the top air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into a vent pipe and even reaching the inside of the top air bag;
s3, quantitatively pressing the air bag: pressing the first lower air bag and/or the second lower air bag to realize the purpose of quantitative pipetting;
s4, discharging and collecting residual liquid: the top bladder is again squeezed so that the remaining liquid of the vent tube and pipette is expelled and collected.
7. The method for blood collection and pipetting by a multi-range quantitative blood collection pipette according to claim 6, wherein in the step S3, the first lower air bag is pressed so that the liquid in the pipette below the horizontal connecting tube connected to the first lower air bag is completely discharged, thereby realizing quantitative pipetting with the maximum range.
8. The method for blood collection and pipetting by a multi-range quantitative blood collection pipette according to claim 6, wherein in the step S3, the second lower air bag is pressed so that the liquid in the pipette below the horizontal connecting tube connected to the second lower air bag is completely discharged, thereby realizing quantitative pipetting.
9. The method for pipetting the blood collection and pipetting of the multi-range quantitative blood collection pipette according to claim 6, wherein in the step S3, the second lower air bag is pressed first, so that the liquid in the pipette below the horizontal connecting pipe connected with the second lower air bag is completely discharged, and quantitative pipetting with one range is realized; and then the first lower air bag is pressed, so that liquid in the pipette between the horizontal connecting pipes connected with the first lower air bag and the second lower air bag is completely discharged, and quantitative pipetting of the other measuring range is realized.
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