CN111841676A - Multi-range quantitative blood sampling pipette and blood sampling and pipetting method thereof - Google Patents
Multi-range quantitative blood sampling pipette and blood sampling and pipetting method thereof Download PDFInfo
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- CN111841676A CN111841676A CN202010853147.0A CN202010853147A CN111841676A CN 111841676 A CN111841676 A CN 111841676A CN 202010853147 A CN202010853147 A CN 202010853147A CN 111841676 A CN111841676 A CN 111841676A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a multi-range quantitative blood sampling pipette and a blood sampling and pipetting method thereof, wherein the pipette comprises a top air bag, a vent pipe, at least one lower air bag, a pipette, at least one horizontal connecting pipe and a micro blood sampling pipe; according to the invention, the micro blood collection tube is arranged below the pipette, and blood is collected by the capillary principle of the micro blood collection tube, so that the function of collecting blood is realized.
Description
Technical Field
The invention relates to a multi-range quantitative blood sampling pipette and a blood sampling and pipetting method thereof.
Background
At present, a quantitative pipette is a pipetting tool for sucking a certain amount of liquid and then transferring the liquid to another place, and a conventional quantitative pipette structure is shown in fig. 1, and includes an air bag 91, a liquid storage bag 92, and a quantitative pipette 93, wherein the air bag 91 is connected to an upper end of the liquid storage bag 92, an upper end of the quantitative pipette 93 is inserted from a lower end of the liquid storage bag 92 and extends into the air bag 91 and the liquid storage bag 92, and the peripheral outer side of the quantitative pipette 93 is kept to be hermetically connected with the peripheral inner side of the lower end of the liquid storage bag 92, so that the quantitative pipette with the existing structure is formed, by squeezing the bladder 91 and then releasing, such that liquid is drawn from the dosing pipette 93 to fill the entire dosing pipette 93, excess liquid will overflow the upper end of the dosing pipette 93 into the lower reservoir 92, then the air bag 91 is squeezed again to discharge the liquid in the quantitative pipette 93, thus forming the whole sucking and pipetting process; however, the liquid overflowing the liquid storage bag 92 cannot be discharged by the squeezing air bag 91, so that a part of liquid sample is wasted, and great cost consumption is caused for expensive samples, and in addition, some novice operators have excessive force for squeezing the air bag 91, so that the quantitative pipette 93, the liquid storage bag 92 and the air bag 91 are completely filled with the liquid, and thus, the air pressure is insufficient, so that the pipetting fails; the structure of the quantitative pipette can derive various deformation structures, for example, chinese patent with publication number CN205126256U, which is to arrange a liquid storage bag in communication with one side of the upper end of the quantitative pipette and prevent backflow through an overflow pipe designed in an inclined manner, so the same drawback exists in the patent, and it is necessary to develop a pipette capable of squeezing, discharging, collecting redundant liquid samples and preventing excessive suction caused by poor control of squeezing pressure by a novice operator; in addition, the existing quantitative pipette only has one measuring range, so that the function is single, and the invention needs to develop a multi-measuring range quantitative pipette; finally, a structure that enables integration of blood collection and pipetting after achieving the above-mentioned problems is also the focus of development of the present invention.
Disclosure of Invention
Aiming at the defects of the prior art, the invention solves the problems that: the multi-range quantitative blood sampling pipette and the blood sampling and pipetting method thereof have the advantages that the blood collection and pipetting are integrated, the redundant liquid samples can be squeezed, discharged and collected, the defect of excessive suction caused by the fact that a novice operator cannot well control the squeezing pressure is overcome, and the multi-range quantitative blood sampling pipette and the blood sampling and pipetting method thereof are provided.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a multi-range quantitative blood sampling pipette comprises a top air bag, an air pipe, at least one lower air bag, a pipette, at least one horizontal connecting pipe and a micro blood sampling pipe; the upper end of the top air bag is closed, and one side of the lower end of the top air bag is provided with a top opening; the lower part of the top air bag is sequentially connected with at least one lower air bag from top to bottom; the upper end of the lower air bag is closed, and one side of the bottom of the lower air bag is provided with a lower opening; a horizontal connecting pipe is horizontally arranged at the lower opening on one side of the bottom of the lower air bag; the pipettes are arranged on the same side of the top air bag and the lower air bag and are distributed in the vertical direction; the upper end of the vent pipe is connected with the top opening on one side of the lower end of the top air bag, and the lower end of the vent pipe is connected with the upper end of the pipette; one end of the horizontal connecting pipe is connected to the lower opening at one side of the bottom of the lower air bag, and the other end of the horizontal connecting pipe is communicated and connected to the side part of the pipette; the horizontal connecting pipe and the pipette are vertically distributed with each other; the micro blood collection tube is arranged below the pipette; the upper end of the micro blood collection tube is inserted into the lower end of the pipette, and the lower end of the micro blood collection tube extends to the lower part of the pipette; the upper end of the micro blood collection tube is communicated with the external air pressure of the pipette.
Further, a cavity with a square-shaped cross section is arranged in the pipette; the cross section of the outer side of the periphery of the micro blood collection tube is of a circular structure; the outer sides of the periphery of the upper end of the micro blood collection tube are connected with the inner walls of the periphery of the cavity of the pipette; a plurality of ventilation gaps are uniformly arranged between the outer sides of the periphery of the micro blood collection tube and the inner wall of the cavity of the pipette.
Furthermore, the periphery of the upper end of the micro blood collection tube is connected with the inner part of the lower end of the pipette in a sealing way; an air vent is formed in one side below the pipette; the air vent extends downwards to be connected with an air nozzle pipe; the lower end face of the air nozzle pipe is positioned above the lower end face of the micro blood collection pipe; the vent is located the top of trace heparin tube up end.
Further, the pipette is a glass tube or a plastic tube.
Further, two lower air bags are connected below the top air bag from top to bottom in sequence; the two lower airbags comprise a first lower airbag and a second lower airbag; the first lower air bag is positioned above the second lower air bag; the first lower air bag and the second lower air bag are respectively communicated and connected with the side part of the pipette through horizontal connecting pipes.
A blood sampling and pipetting method of a multi-range quantitative blood sampling pipette comprises the following steps:
s1, blood sampling: contacting the lower end of the micro blood collection tube below the pipette with blood to be collected, sucking the blood through the capillary action of the micro blood collection tube until the whole micro blood collection tube is filled, and then pressing the top air bag or the lower air bag to squeeze the blood in the micro blood collection tube into a diluent;
s2, pressing of the top airbag: firstly, pressing the top air bag, then contacting the lower end of the pipette with the diluent to be sucked, loosening the top air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into a vent pipe and even reaching the top air bag;
s3, quantitative pressing air bag: the first lower air bag and/or the second lower air bag are pressed to realize the quantitative liquid transfer;
s4, discharging and collecting residual liquid: the top balloon is squeezed again so that the vent tube and the remaining liquid from the pipette are expelled and collected.
Further, in step S3, the first lower bladder is pressed so that all the liquid in the pipette below the horizontal connection tube connected to the first lower bladder is discharged, thereby achieving quantitative liquid transfer in the maximum range.
Further, in step S3, the second lower air bag is pressed so that all the liquid in the pipette below the horizontal connection tube connected to the second lower air bag is discharged, thereby realizing quantitative liquid transfer.
Further, in step S3, the second lower air bag is first pressed so that all the liquid in the pipette below the horizontal connection tube connected to the second lower air bag is discharged, thereby achieving quantitative liquid transfer in one range; then the first lower air bag is pressed, so that the liquid in the pipette between the horizontal connecting pipes connected with the first lower air bag and the second lower air bag is completely discharged, and quantitative pipetting of another measuring range is realized.
The invention has the advantages of
1. According to the invention, the micro blood collection tube is arranged below the pipette, and blood is collected by the capillary principle of the micro blood collection tube, so that the function of collecting blood is realized.
2. The lower end of the top air bag and the upper end of the lower air bag are sealed, the top air bag is directly ventilated with the upper end of the pipette through the vent pipe, the lower air bag is ventilated with the side part of the pipette through the horizontal connecting pipe, so that the top air bag and the lower air bag are provided with independent and non-interfering paths to be ventilated with the pipette, liquid is sucked through the top air bag, each lower air bag discharges liquid to achieve the purpose of quantitative liquid transfer, and redundant liquid samples are discharged through extrusion of the top air bag to achieve recycling and avoid waste; because the top air bag and the lower air bag are provided with independent paths which do not interfere with each other and are used for ventilating with the pipette, even if the top air bag is excessively extruded and filled with the whole liquid, the quantitative liquid drainage of each lower air bag is not influenced; when the lower air bags are sequentially connected with the upper air bag from top to bottom below the top air bag, the invention has the advantages of multiple ranges and more flexible and changeable use.
Drawings
Fig. 1 is a schematic structural diagram of the prior art.
Fig. 2 is a schematic view showing the structure of the present invention with a lower bag.
Fig. 3 is a schematic view showing the structure of the present invention having two lower air bags.
Fig. 4 is a schematic view showing a structure of the present invention having three lower airbags.
FIG. 5 is a schematic front view showing the structure of a micro blood collection tube and a pipette in embodiment 1 of the present invention.
FIG. 6 is a schematic sectional view showing the structure of a micro blood collection tube and a pipette in embodiment 1 of the present invention.
FIG. 7 is a schematic front view showing the structure of a micro blood collection tube and a pipette in embodiment 2 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1
As shown in fig. 2, 5 and 6, a multi-range quantitative blood sampling pipette comprises a top air bag 1, a vent pipe 3, at least one lower air bag 2, a pipette 4, at least one horizontal connecting pipe 5 and a micro blood sampling pipe 9; the upper end of the top airbag 1 is closed, and a top opening 11 is formed in one side of the lower end of the top airbag 1; the lower part of the top airbag 1 is sequentially connected with at least one lower airbag 2 from top to bottom; the upper end of the lower air bag 2 is closed, and one side of the bottom of the lower air bag 2 is provided with a lower opening 21; a horizontal connecting pipe 5 is horizontally arranged at a lower opening 21 at one side of the bottom of the lower air bag 2; the pipettes 4 are arranged on the same side of the top air bag 1 and the lower air bag 2 and are distributed in the vertical direction; the upper end of the vent pipe 3 is connected with the top opening 11 at one side of the lower end of the top airbag 1, and the lower end of the vent pipe 3 is connected with the upper end of the pipette 4; one end of the horizontal connecting pipe 5 is connected to the lower opening 21 at one side of the bottom of the lower air bag 2, and the other end of the horizontal connecting pipe 5 is communicated and connected to the side part of the pipette 4; the horizontal connecting tube 5 and the pipette 4 are distributed vertically to each other. Further, the pipette 4 is a glass or plastic tube, the inside of the pipette 4 is a generally circular cavity structure, and the pipette capacity is determined by the inner diameter and length thereof. At least one scale mark is arranged on the pipette 4; the scale marks are horizontally aligned with the upper end surface of the inner cavity of the horizontal connecting pipe 5. And the top air bag 1, the lower air bag 2, the vent pipe 3 and one side above the pipette 4 are connected through a plastic piece 6 in a curing manner. In the present embodiment, a lower airbag 2 is optionally connected below the top airbag 1. The upper end of the micro blood collection tube 9 is inserted into the lower end of the pipette 4, and the lower end of the micro blood collection tube 9 extends to the lower part of the pipette 4; the upper end of the micro blood collection tube 9 is communicated with the external air pressure of the pipette 4. Further preferably, a cavity with a square-shaped cross section is arranged inside the pipette 4; the section of the outer side of the periphery of the micro blood collection tube 9 is of a circular structure; the outer side of the periphery of the upper end of the micro blood collection tube 9 is connected with the inner wall of the periphery of the cavity of the pipette 4; evenly be equipped with a plurality of ventilation gap 41 between the outside all around of micro blood collection tube 9 and the cavity inner wall of pipette 4, so this embodiment is ventilated with the outside through a plurality of ventilation gap 41 between the outside all around of micro blood collection tube 9 and the cavity inner wall of pipette 4 to micro blood collection tube 9 utilizes the capillary principle to take a blood sample.
In the pipetting process of the embodiment, two modes can be adopted, wherein the first mode is that the top air bag 1 is directly extruded to aspirate liquid, then the lower air bag 2 is extruded to quantitatively pipette liquid, and finally the top air bag 1 is extruded again to discharge residual liquid for recycling; the second is directly extruding the lower air bag 2 to suck liquid, then quantitatively transferring liquid by extruding the top air bag 1, and finally extruding the lower air bag 2 again to discharge residual liquid for recovery.
Example 2
Referring to example 1, this example differs from example 3 and example 7 in the structure of ventilation of the micro blood collection tube 9, the number of lower air cells 2, and the method of pipetting, in that two lower air cells 2 are connected to the bottom of the top air cell 1 in this order; the two lower airbags 2 include a first lower airbag 2a and a second lower airbag 2 b; the first lower bag 2a is positioned above the second lower bag 2 b; the first lower air bag 2a and the second lower air bag 2b are respectively communicated and connected with the side part of a pipette 4 through a horizontal connecting pipe 5; the periphery of the upper end of the micro blood collection tube 9 is connected with the inner part of the lower end of the pipette 4 in a sealing way; an air vent 41 is formed at one side below the pipette 4; the air port 41 is connected with an air nozzle pipe 42 in a downward extending way; the lower end face of the air nozzle tube 42 is positioned above the lower end face of the micro blood collection tube 9; the vent 41 is located above the upper end surface of the micro blood collection tube 9. In this embodiment, the upper end of the micro blood collection tube 9 is ventilated to the outside through the vent hole 41 on the pipette 4 side.
A blood sampling and pipetting method of a multi-range quantitative blood sampling pipette comprises the following steps:
s1, blood sampling: the lower end of the micro blood collection tube 9 below the pipette 4 is contacted with blood to be collected, the blood is sucked by the capillary action of the micro blood collection tube 9 until the whole micro blood collection tube 9 is filled, and then the blood in the micro blood collection tube 9 is squeezed into the diluent by pressing the top air bag 1 or the lower air bag 2.
S2, pressing of the top airbag: the top air bag 1 is first pressed and then the lower end of the pipette 4 is brought into contact with the dilution liquid to be aspirated, the top air bag 1 is released, liquid is aspirated from the lower end of the pipette 4 and fills the entire pipette 4, and liquid is aspirated into the vent tube 3 and even into the top air bag 1.
S3, quantitative pressing air bag: the first lower air bag 2a and/or the second lower air bag 2b are pressed to realize the purpose of quantitative liquid transfer; the step has three modes for quantitative pipetting, and can be selected according to actual requirements. Firstly, the first lower air bag 2a is pressed, so that the liquid in the pipette 4 below the horizontal connecting pipe 5 connected with the first lower air bag 2a is completely discharged, and quantitative liquid transfer with the maximum range is realized; secondly, the second lower air bag 2b is pressed, so that the liquid in the pipette 4 below the horizontal connecting pipe 5 connected with the second lower air bag 2b is completely discharged, and quantitative liquid transfer is realized; thirdly, the second lower air bag 2b is firstly pressed, so that the liquid in the pipette below the horizontal connecting pipe 5 connected with the second lower air bag 2b is completely discharged, and quantitative liquid transfer of one measuring range is realized; then, the first lower air bag 2a is pressed, so that the liquid in the pipette 4 between the horizontal connecting tubes 5 connected with the first lower air bag 2a and the second lower air bag 2b is completely discharged, and quantitative pipetting of another range is realized.
S4, discharging and collecting residual liquid: the top balloon 1 is squeezed again so that the remaining liquid in the vent tube 3 and the pipette 4 is expelled and collected.
The present invention is exemplified by two embodiments, but not limited thereto, and it is possible to connect three lower airbags 2, four lower airbags 2, five lower airbags 2, and the like from the top to the bottom in order below the top airbag 1 as shown in fig. 4 with more range as referring to embodiment 2.
The lower end of a top air bag 1 and the upper end of a lower air bag 2 are sealed, the top air bag 1 is directly ventilated with the upper end of a pipette 4 through a ventilating pipe 3, the lower air bag 2 is ventilated with the side part of the pipette 4 through a horizontal connecting pipe 5, so that the top air bag 1 and the lower air bag 2 are provided with independent and non-interfering paths to be ventilated with the pipette, liquid is sucked through the top air bag 1, each lower air bag 2 discharges liquid to achieve the quantitative liquid transferring purpose, and redundant liquid samples are discharged through extrusion of the top air bag 1 to achieve recycling and avoid waste; because the top air bag 1 and the lower air bag 2 are provided with independent paths which do not interfere with each other and are used for ventilating with the pipette, even if the top air bag 1 is excessively extruded and filled with the whole liquid, the quantitative liquid drainage of each lower air bag 2 is not influenced; when the lower air bags 2 are sequentially connected with the lower air bags 1 from top to bottom, the invention has the advantages of multiple ranges and more flexible and variable use.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A multi-range quantitative blood sampling pipette is characterized by comprising a top air bag, a vent pipe, at least one lower air bag, a pipette, at least one horizontal connecting pipe and a micro blood sampling pipe; the upper end of the top air bag is closed, and one side of the lower end of the top air bag is provided with a top opening; the lower part of the top air bag is sequentially connected with at least one lower air bag from top to bottom; the upper end of the lower air bag is closed, and one side of the bottom of the lower air bag is provided with a lower opening; a horizontal connecting pipe is horizontally arranged at the lower opening on one side of the bottom of the lower air bag; the pipettes are arranged on the same side of the top air bag and the lower air bag and are distributed in the vertical direction; the upper end of the vent pipe is connected with the top opening on one side of the lower end of the top air bag, and the lower end of the vent pipe is connected with the upper end of the pipette; one end of the horizontal connecting pipe is connected to the lower opening at one side of the bottom of the lower air bag, and the other end of the horizontal connecting pipe is communicated and connected to the side part of the pipette; the horizontal connecting pipe and the pipette are vertically distributed with each other; the micro blood collection tube is arranged below the pipette; the upper end of the micro blood collection tube is inserted into the lower end of the pipette, and the lower end of the micro blood collection tube extends to the lower part of the pipette; the upper end of the micro blood collection tube is communicated with the external air pressure of the pipette.
2. The multi-range quantitative blood collection pipette of claim 1, wherein the pipette is internally provided with a cavity having a square cross-section; the cross section of the outer side of the periphery of the micro blood collection tube is of a circular structure; the outer sides of the periphery of the upper end of the micro blood collection tube are connected with the inner walls of the periphery of the cavity of the pipette; a plurality of ventilation gaps are uniformly arranged between the outer sides of the periphery of the micro blood collection tube and the inner wall of the cavity of the pipette.
3. The multi-range quantitative blood collection pipette according to claim 1, wherein the periphery of the upper end of the micro blood collection tube is hermetically connected with the inside of the lower end of the pipette; an air vent is formed in one side below the pipette; the air vent extends downwards to be connected with an air nozzle pipe; the lower end face of the air nozzle pipe is positioned above the lower end face of the micro blood collection pipe; the vent is located the top of trace heparin tube up end.
4. The multi-range quantitative blood collection pipette of claim 1, wherein the pipette is a glass or plastic tube.
5. The multi-range quantitative blood collection pipette according to claim 1, wherein two lower air bags are connected below the top air bag from top to bottom in sequence; the two lower airbags comprise a first lower airbag and a second lower airbag; the first lower air bag is positioned above the second lower air bag; the first lower air bag and the second lower air bag are respectively communicated and connected with the side part of the pipette through horizontal connecting pipes.
6. A blood sampling pipetting method of the multirange quantitative blood sampling pipette according to claim 5, characterized by comprising the steps of:
s1, blood sampling: contacting the lower end of the micro blood collection tube below the pipette with blood to be collected, sucking the blood through the capillary action of the micro blood collection tube until the whole micro blood collection tube is filled, and then pressing the top air bag or the lower air bag to squeeze the blood in the micro blood collection tube into a diluent;
s2, pressing of the top airbag: firstly, pressing the top air bag, then contacting the lower end of the pipette with the diluent to be sucked, loosening the top air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into a vent pipe and even reaching the top air bag;
s3, quantitative pressing air bag: the first lower air bag and/or the second lower air bag are pressed to realize the quantitative liquid transfer;
s4, discharging and collecting residual liquid: the top balloon is squeezed again so that the vent tube and the remaining liquid from the pipette are expelled and collected.
7. The method for blood sampling and pipetting by a multirange quantitative blood sampling pipette of claim 6, wherein in step S3, the first lower air bag is pressed so that all the liquid in the pipette up to the horizontal connection tube connected to the first lower air bag is discharged, thereby realizing quantitative pipetting in the maximum range.
8. The method for blood collection and pipetting by a multirange quantitative blood collection pipette of claim 6, wherein in step S3, the second lower air bag is pressed so that all the liquid in the pipette below the horizontal connection tube connected to the second lower air bag is discharged to achieve quantitative pipetting.
9. The method for pipetting a blood collected by a multi-range quantitative blood collection pipette according to claim 6, wherein in step S3, the second lower air bag is first pressed so that the liquid in the pipette below the horizontal connection tube connected to the second lower air bag is completely discharged to perform quantitative pipetting in one range; then the first lower air bag is pressed, so that the liquid in the pipette between the horizontal connecting pipes connected with the first lower air bag and the second lower air bag is completely discharged, and quantitative pipetting of another measuring range is realized.
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